Jilin Tang

Chinese Academy of Sciences, Peping, Beijing, China

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Publications (43)162.99 Total impact

  • [show abstract] [hide abstract]
    ABSTRACT: Human gonadotropin-releasing hormone receptor (GnRH-R; or type I GnRH-R) expressed in tumor cells has gained more and more attention as a distinct target for cancer therapy. Given the diverse clinical utility, improved characterization of the subcellular distribution and organization of GnRH-R is an important step in the development of more effective and possibly new therapeutic strategies. In the present study, the nano-organization of human GnRH-R was analyzed on human bladder cancer cell (T24) by atomic force microscopy (AFM). The recognition images reveal that GnRH-Rs (nano-domains or single GnRH-R) are irregularly distributed on the T24 cell surface with the sizes from ~ 4 to ~ 370 nm. The locations of the GnRH-Rs were identified on the topographical images with nanometer accuracy. The obtained results enrich our understanding of the local distribution of GnRH-Rs on T24 cell membrane and show the ability of biological AFM to obtain more complete and exact information on intact cell surface.
    Analytical Chemistry 01/2014; · 5.70 Impact Factor
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    ABSTRACT: A label-free detection method of kanamycin using aptamer-based cantilever array sensor was developed. The cantilever array was composed of sensing cantilevers and reference cantilevers. This configuration allowed direct detection of individual cantilever deflections and subsequent determination of differential deflection of sensing/reference cantilever pair. The sensing cantilevers were functionalized with kanamycin aptamer, which was used as receptor molecules while the reference cantilevers were modified with 6-mercapto-1-hexanol (MCH) to eliminate the influence of environmental disturbances. The kanamycin-aptamer interaction induced a change in cantilever surface stress, which caused a differential deflection between the sensing and reference cantilever pair. The surface stress change was linear with kanamycin concentration over the range of 100μM-10mM with a correlation coefficient of 0.995. A detection limit of 50μM was obtained, at a signal-to-noise ratio of 3. The sensor also showed good selectivity against other antibiotics such as neomycin, ribostamycin and chloramphenicol. The facile method for kanamycin detection may have great potential for investigating more other molecules.
    Biosensors & bioelectronics 01/2014; 56C:112-116. · 5.43 Impact Factor
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    ABSTRACT: A facile two-step method was developed for a large-scale growth of hierarchical MnCo2O4.5 nanostructure modified MnOOH nanorods (MC hybrid materials) as an efficient catalyst for water treatment. The synthesis involved a one-step hydrothermal process to prepare MnOOH nanorods and subsequently a simple solution method using hydrothermally synthesized MnOOH nanorods as both the template and Mn source to obtain MnCo2O4.5/MnOOH (MC) hybrid materials. The as-prepared MC hybrid materials with hierarchical structures could provide more active sites for catalytic degradation of methylene blue. These results indicate that the designed MC hybrid materials exhibit a promising capability for the degradation of dyes.
    Catalysis Communications 01/2014; 46:174–178. · 2.92 Impact Factor
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    ABSTRACT: A facile process was developed for the synthesis of graphene-supported TiO2 (B) nanosheets (GTBN) composite based on the hydrothermal treatment titanium (III) chloride and graphene oxide in an ethylene glycol. The morphology and microstructure of the composites were examined by X-ray diffraction, X-ray photoelectron spectroscopy, transmission electron microscopy, and Raman spectroscopy. The obtained GTBN show a high thermal stability and the phase transformation of TiO2 (B) to anatase can be prevented by graphene after pyrolysis of GTBN at 350°C for 2h. Furthermore, GTBN exhibited high rate performance and stability of lithium ion batteries, due to the enhanced conductivity of the electrode and accommodation to volume/strain changes during lithium insertion-extraction.
    Journal of Colloid and Interface Science 08/2013; · 3.17 Impact Factor
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    ABSTRACT: The understanding of ligand binding interactions is an important component of understanding the fundamental mechanism of receptor function. In this study, the binding abilities of EGF and TGF-α to EGFR on human bladder cancer (T24) cells were investigated by single molecular force spectroscopy (SMFS) based on atomic force microscopy (AFM). By approaching the specifically functionalized AFM tips to the T24 cell surface and subsequent retraction, specific unbinding events of the EGF/EGFR complexes and TGF-α/EGFR complexes were investigated. Further, the unbinding forces and the kinetic off rate constants that govern the bond stabilities were calculated through varying the external mechanic forces applied. Meanwhile, the distances from the energy minimum to the transition states along the separation paths of the EGF/EGFR complexes and TGF-α/EGFR complexes were deduced. This study at single-molecule level may enrich our understanding of the ligand binding properties of EGFR and provide some new information to the development of improved EGFR inhibitors. In addition, the results present new insight into the study of the energy landscape of the dissociation of ligand-EGFR system.
    The Analyst 07/2013; · 4.23 Impact Factor
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    ABSTRACT: We report a simple and sensitive method for label-free detection of single-stranded DNA-binding protein (SSBP) based on an array of microfabricated cantilevers. The single-stranded DNA (ssDNA) was immobilized on the surface of the sensing cantilevers to detect SSBP, while the reference cantilevers were modified with 6-mercapto-1-hexanol to detect any unwanted cantilever deflection. The differential deflection signals that reveal specific SSBP-ssDNA binding have been found to depend on the SSBP concentration. Using the cantilever array sensor we can detect SSBP in the concentration range from 0.01 to 7μgmL(-1). Other proteins, such as thrombin or bovine serum albumin induced no significant deflection of the cantilevers. Our results show the potential for the application of cantilever array sensor system as a powerful tool to detect proteins with high sensitivity and specificity.
    Talanta 05/2013; 109:173-6. · 3.50 Impact Factor
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    ABSTRACT: A facile two step process was developed for the synthesis of porous Co3O4 nanorods- reduced graphene oxide (PCNG) hybrid materials based on the hydrothermal treatment cobalt acetate tetrahydrate and graphene oxide in a glycerol-water mixed solvent, followed by annealing the intermediate of reduced graphene oxide-supported Co(CO3)0.5(OH)•0.11H2O nanorods in a N2 atmosphere. The morphology and microstructure of the composites were examined by X-ray diffraction, X-ray photoelectron spectroscopy, transmission electron microscopy and Raman spectroscopy. It is shown that the obtained PCNG have intrinsic peroxidase-like activity. The PCNG are utilized for the catalytic degradation of methylene blue. The good catalytic performance of the composites could be attributed to the synergy between the functions of porous Co3O4 nanorods and reduced graphene oxide.
    ACS Applied Materials & Interfaces 04/2013; · 5.01 Impact Factor
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    ABSTRACT: A facile method is proposed for the synthesis of three-dimensional (3D) flower-like Co3-xFexO4 ferrite (CF) hollow spheres, using SiO2@FeOOH as precursor. The CF hollow spheres are efficient for the catalytic degradation of methylene blue (MB) in the presence of H2O2 at 80 °C. The obtained CF hollow spheres were characterized using transmission electron microscopy, field emission scanning electron microscopy, X-ray diffraction, X-ray photo-electron spectroscopy, and N2 adsorption-desorption isotherm measurements. The formation of 3D hierarchical flower-like superstructure was influenced by the relative amount of urea used. As the mole ratio of CoCl2 and urea decreased, the structure of the products was tailored from yolk-like spheres to hollow spheres with different sized void interiors. Moreover, N2 adsorption-desorption isotherm analysis showed that the CF hollow spheres have a large specific surface area (163 m2 g-1) which provided more activity sites. The CF hollow spheres can catalyze the oxidation of MB efficiently. These results indicate that the designed CF hollow spheres exhibit promising capability for the degradation of dyes.
    Nanoscale 03/2013; · 6.23 Impact Factor
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    ABSTRACT: A facile method is proposed for the synthesis of cubic CoFe2O4 ferrite–reduced graphene oxide nanocomposite sheets (rGO–CFs), using poly(N-vinyl-2-pyrrolidone) as the reductant and stabilizer. The rGO–CFs functioned as efficient peroxidase mimetics and were successfully applied for colorimetric assay. The morphology and composition of the rGO–CFs were characterized by transmission electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction. Significantly, the peroxidase-like activity of the rGO–CFs followed typical Michaelis–Menten kinetics and showed a good affinity to 3,3,5,5-tetramethyl benzidine. Under optimum conditions, the colorimetric assay showed a lower detection limit (S/N = 3) of 0.3 μM when compared with that of other nanoparticle based colorimetric assays. Furthermore, the cubic nanostructured rGO–CFs exhibited better stability than horseradish peroxidase when they were exposed to solutions with different solvents and temperatures. These excellent properties made the cubic nanostructured rGO–CFs an ideal candidate for a wide range of potential applications as peroxidase mimetics.
    J. Mater. Chem. A. 03/2013; 1(13):4352-4357.
  • Xi Ke, Jilin Tang
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    ABSTRACT: The fabrication of patterned microstructures in poly(dimethylsiloxane) (PDMS) is a prerequisite for soft lithography. Herein, curvilinear surface relief microstructures in PDMS are fabricated through a simple three-stage approach combining microcontact printing (μCP), selective surface wetting/dewetting and replica molding (REM). First, using an original PDMS stamp (first-generation stamp) with linear relief features, a chemical pattern on gold substrate is generated by μCP using hexadecanethiol (HDT) as an ink. Then, by a dip-coating process, an ordered polyethylene glycol (PEG) polymer-dot array forms on the HDT-patterned gold substrate. Finally, based on a REM process, the PEG-dot array on gold substrate is used to fabricate a second-generation PDMS stamp with microcavity array, and the second-generation PDMS stamp is used to generate third-generation PDMS stamp with microbump array. These fabricated new-generation stamps are utilized in μCP and in micromolding in capillaries (MIMIC), allowing the generation of surface micropatterns which cannot be obtained using the original PDMS stamp. The method will be useful in producing new-generation PDMS stamps, especially for those who want to use soft lithography in their studies but have no access to the microfabrication facilities.
    ChemPhysChem 02/2013; · 3.35 Impact Factor
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    ABSTRACT: We present a new method for specific detection of oxytetracycline (OTC) at nanomolar concentrations based on a microfabricated cantilever array. The sensing cantilevers in the array are functionalized with self-assembled monolayers (SAMs) of OTC-specific aptamer, which acts as a recognition molecule for OTC. While the reference cantilevers in the array are functionalized with 6-mercapto-1-hexanol SAMs to eliminate the influence of environmental disturbances. The cantilever sensor shows a good linear relationship between the deflection amplitude and the OTC concentration in the range of 1.0-100 nM. The detection limit of the cantilever array sensor is as low as 0.2 nM, which is comparable to some traditional methods. Other antibiotics such as doxycycline and tetracycline do not cause significant deflection of the cantilevers. It is demonstrated that the cantilever array sensors can be used as a powerful tool to detect drugs with high sensitivity and selectivity.
    Analytical Chemistry 01/2013; · 5.70 Impact Factor
  • Biophysical Journal 01/2013; 104(2):381-. · 3.67 Impact Factor
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    ABSTRACT: Molecular recognition force spectroscopy (MR-FS) was applied to investigate the dynamic interaction between aptamer GBI-10 and tenascin-C (TN-C) on human glioblastoma cell surface at single-molecule level. The unbinding force between aptamer GBI-10 and TN-C was 39 pN at the loading rate of 0.3 nN sec(-1) . A series of kinetic parameters concerning interaction process such as the unbinding force f(u) , the association rate constant k(on) , dissociation rate constant at zero force k(off) , and dissociation constant K(D) for aptamer GBI-10/TN-C complexes were acquired. In addition, the interaction of aptamer GBI-10 with TN-C depended on the presence of Mg(2+) . This work demonstrates that MR-FS can be used as an attractive tool for exploring the interaction forces and dynamic process of aptamer and ligand at the single-molecule level. As a future perspective, MR-FS may be used as a potential diagnostic and therapeutic tool by combining with other techniques. Copyright © 2012 John Wiley & Sons, Ltd.
    Journal of Molecular Recognition 01/2013; 26(1):46-50. · 3.01 Impact Factor
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    ABSTRACT: The capability of Bacillus subtilis spores to withstand extreme environmental conditions is thought to be conferred especially by their outermost proteinaceous protective layer, called the spore coat. Of the over 70 proteins that form the spore coat, only a small subset of them affect its morphogenesis, they are referred to as morphogenetic proteins. In this study we investigated the interaction between two spore coat morphogenetic proteins SpoVID and CotE. SpoVID is involved in the process of spore surface encirclement by individual coat proteins, these include CotE, which controls the assembly of the outer coat layer. Both proteins were proposed to be recruited to a common protein scaffold, but their direct association has not been previously shown. Here we studied the interactions between CotE and SpoVID in vitro for the first time by using molecule recognition force spectroscopy, which allows the detection of piconewton forces between conjugated biological pairs and also facilitates the investigation of dynamic processes. The most probable CotE-CotE unbinding force was 49.4±0.1 pN at a loading rate of 3.16×10(3) pN/s while that of SpoVID-CotE was 26.5±0.6 pN at a loading rate of 7.8 × 10(2) pN/s. We further analyzed the interactions with the bacterial two hybrid system and pull down experiments, which also indicate that SpoVID interacts directly with CotE. In combination with the previously identified direct contacts among SpoIVA, SpoVID and SafA, our data imply that the physical association of key morphogenetic proteins forms a basic skeleton where other coat proteins could be attached.
    Journal of Structural Biology 11/2012; · 3.36 Impact Factor
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    ABSTRACT: Mussels have been shown to attach to virtually all types of inorganic and organic surfaces via their adhesive proteins. The adhesive proteins secreted by mussels contain high concentrations of catechol and amine functional groups, which have similar functional groups with polydopamine (PDA). Inspired by mussels, a mild and environmentally friendly method was used to synthesize Ag nanoparticles (Ag NPs) on functionalized PDA-graphene nanosheets (PDA-GNS) with uniform and high dispersion. First, a uniform layer of PDA was coated on graphene oxide (GO) by polymerizing dopamine (DA) at room temperature. During the process GO was reduced by the DA. The PDA layer on the surface of GNS can be used as a nanoscale guide to form uniform Ag NPs on the surface of PDA-GNS. The obtained Ag-PDA-GNS hybrid materials are characterized by atomic force microscopy, transmission electron microscopy, UV-vis spectroscopy, Raman spectroscopy, X-ray photo-electron spectroscopy, X-ray diffraction, and thermal gravimetric analysis. The resultant Ag-PDA-GNS hybrid materials exhibited strong antibacterial properties to both Gram-negative and Gram-positive bacteria due to the synergistic effect of GNS and Ag NPs.
    Nanoscale 11/2012; · 6.23 Impact Factor
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    ABSTRACT: Luteinizing hormone-releasing hormone-Pseudomonas aeruginosa exotoxin 40 (LHRH-PE40), is a candidate target drug associated with elevated LHRH receptor (LHRH-R) expression in malignant tumor tissue. The capability of LHRH-PE40 to recognize LHRH-Rs on living cell membrane was studied with single molecular recognition force spectroscopy (SMFS) based on atomic force microscopy (AFM). The recognition force of LHRH-PE40/LHRH-R was compared with that of LHRH/LHRH-R by dynamic force spectroscopy. Meanwhile, cell growth inhibition assay and fluorescence imaging were presented as complementary characterization. The results show that LHRH moiety keeps its capability to recognize LHRH-R specifically, which imply that recombinant protein LHRH-PE40 can be a promising target drug.
    The Journal of Physical Chemistry B 10/2012; · 3.61 Impact Factor
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    ABSTRACT: Single-walled carbon nanohorns (SWNHs), distinguished by their high purity and unique structure, were noncovalently functionalized with Schiff-base cobalt(II) (Co-salen) for the first time. Cyclic voltammogram of Co-salen/SWNHs display a pair of redox peaks with a formal potential of +0.15 V in 0.1 M phosphate buffer solution (pH 7.0). A novel bifunctional electrochemical sensor for hydrazine oxidation and hydrogen peroxide reduction are presented based on the composite modified electrode. Using amperometry, the linear relationship of hydrazine was found to be in the range from 1 to 96 μM with a sensitivity of 2.4 μA mM−1, the detection limit is 0.1 μM (S/N = 3). The linear relationship of hydrogen peroxide is in the range from 50 to 4600 μM with a sensitivity of 1.8 μA mM−1, the detection limit is 10 μM (S/N = 3). The hybrid material provides a good electrochemical sensing platform and has potential applications in environment analysis and biosensors.
    Analytical methods 10/2012; 4(11):3580-3585. · 1.86 Impact Factor
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    ABSTRACT: Scatter me: A fast and cost-effective approach for the fabrication of surface-enhanced Raman scattering (SERS) arrays is developed. The method applied combines microcontact printing, electrodeposition, and galvanic replacement without the need for expensive instruments and intricate processing. The as-prepared arrays show excellent SERS activity and high reproducibility for Rhodamine 6G.
    ChemPhysChem 09/2012; · 3.35 Impact Factor
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    ABSTRACT: Fluorescent carbon dots (C-dots) are prepared directly via a simple hydrothermal method using bovine serum albumin (BSA) as a carbon source in the presence of surface passivation reagents. The obtained C-dots have low cytotoxicity and good biocompatibility, demonstrating that their features are good for application in cell imaging.
    RSC Advances 09/2012; 2(23):8599-8601. · 2.56 Impact Factor
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    ABSTRACT: The self-assembled structures possess superior stability, biocompatibility and mechanical strength, and their study can provide insight into the use of creating novel biomaterials. Atomic force microscopy (AFM) images of single-stranded DNA (ssDNA) nanostructures show that well-ordered organization, high homogeneity, and molecular dimensions fractal-shaped fibers formed on a gold substrate covered with self-assembled monolayers (SAMs) of 1-hexadecanethiol (HDT). The nanoscaled architectures of ssDNA on HDT/Au changed remarkably following the process of diffusion-limited cluster aggregation (DLA) over time. The ssDNA fibers prefer to form on hydrophobic SAMs instead of hydrophilic SAMs, and the ssDNA has to have complementary regions in their sequences. This method might not be used only for the construction of fractal patterns, but also for the design and fabrication of functional DNA-based, self-assembled materials that exhibit self-similarity at multiple length scales.
    The Journal of Physical Chemistry B 09/2012; 116(38):11594-9. · 3.61 Impact Factor

Publication Stats

165 Citations
162.99 Total Impact Points


  • 2011–2014
    • Chinese Academy of Sciences
      • State Key Laboratory of Electroanalytical Chemistry
      Peping, Beijing, China
    • Anhui University
      • School of Chemistry and Chemical Engineering
      Hefei, Anhui Sheng, China
  • 2013
    • Nanchang University
      Nan-ch’ang-shih, Jiangxi Sheng, China
  • 2010–2013
    • Northeast Institute of Geography and Agroecology
      • State Key Laboratory of Electroanalytical Chemistry
      Beijing, Beijing Shi, China
  • 2006–2012
    • Johannes Kepler University Linz
      • Institut für Biophysik
      Linz, Upper Austria, Austria