Mustafa Celik

Ankara University, Ankara, Ankara, Turkey

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Publications (16)32.4 Total impact

  • [show abstract] [hide abstract]
    ABSTRACT: Abstract The genotoxic potential of rosuvastatin as one of the statin drugs was assessed by chromosomal aberrations (CAs), micronucleus (MN) and DNA damage by comet assay in the human peripheral blood lymphocytes. Rosuvastatin was used at concentrations of 0.0625, 0.125, 0.25, 0.5 and 1 µg/mL for these in vitro assays. In all assays, a negative and positive control were also included. CA frequencies were significantly increased in all concentrations at 24 hours and significantly increased in all concentrations except 0.0625 µg/mL at 48 hours, compared to the negative control. Rosuvastatin has a decreased mitotic index (MI) at 0.5- and 1-µg/mL concentrations at 24 hours and at 0.25, 0.5 and 1 µg/mL at 48 hours. A significant increase was observed for induction of MN in all treatments, compared to the negative control. Cytokinesis-block proliferation indices were not affected by treatments with rosuvastatin. In the comet assay, significant increases in comet tail length and tail moment were observed at 0.0625-, 0.5- and 1-µg/mL concentrations. Comet intensity was significantly increased in all concentrations except 0.0625 µg/mL. According to these results, rosuvastatin is cytotoxic and clastogenic/aneugenic in human peripheral lymphocytes. Further studies should be conducted in other test systems to evaluate the full genotoxic potential of rosuvastatin.
    Drug and Chemical Toxicology 11/2013; · 1.29 Impact Factor
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    ABSTRACT: In vitro genotoxic effects of antioxidant additives, such as citric acid (CA) and phosphoric acid (PA) and their combination, as well as antimicrobial additives, such as benzoic acid (BA) and calcium propionate (CP), on human lymphocytes were determined using alkaline single-cell gel electrophoresis. There was a significant increase in the DNA damage in human lymphocytes after 1 h of in vitro exposure to CA, PA, BA and CP (200, 25-200, 50-500, 50-1000 μg/mL, respectively). The combination of CA and PA significantly increased the mean tail intensity at all the concentrations used (25-200 μg/mL) and significantly increased the mean tail length mainly after higher concentrations (100 and 200 μg/mL). Data in this study showed that the concentrations of food additives used induce DNA damage and PA was the most genotoxic and CA was less genotoxic additives among them.
    Toxicology and Industrial Health 11/2012; · 1.56 Impact Factor
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    ABSTRACT: In vitro genotoxic effects of organophosphorus insecticides Phorate (PHR) and Trichlorfon (TCF) were investigated using four genotoxicity endpoints. Different concentration ranges between 0.25-2.00 μg mL(-1) of PHR and 2.34-37.50 μg mL(-1) of TCF were applied to lymphocytes. PHR and TCF significantly increased the frequency of chromosomal aberrations (except 2.34 μg mL(-1) for TCF) and sister chromatid exchanges at all treatment times and concentrations. Most of the used concentrations induced a significant increase in the frequency of micronuclei. Furthermore, PHR and TCF significantly decreased the mitotic index at the higher concentrations after 24- and 48-h treatments. In the comet assay, PHR and TCF significantly increased the comet tail at all concentrations. However, the comet tail intensity was significantly increased at only the highest concentration of PHR and at all concentrations of TCF. According to these results, PHR and TCF possess clastogenic, mutagenic, and DNA damaging effects in human lymphocytes in vitro. © 2012 Wiley Periodicals, Inc. Environ Toxicol, 2012.
    Environmental Toxicology 05/2012; · 2.71 Impact Factor
  • Mustafa Celik, Hüseyin Aksoy, Serkan Yilmaz
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    ABSTRACT: Beauvericin, a naturally occurring contaminants of food and feeds, has been implicated in several mycotoxicoses; however, there is little information on its genotoxicity. Therefore, the genotoxic and cytotoxic effects of beauvericin in in vitro cultures of human lymphocytes were investigated with chromosome aberrations (CAs), sister-chromatid exchanges (SCEs), micronuclei (MN) as well as mitotic, proliferative and nuclear division indices. Beauvericin caused a significant concentration-dependent increase in chromosomal aberrations, sister-chromatid exchanges and micronuclei. It also significantly decreased the mitotic index at the two highest concentrations. However, no significant change in the proliferative and nuclear division indices was found. The results indicated that BEA is genotoxic to human lymphocytes in vitro.
    Ecotoxicology and Environmental Safety 10/2010; 73(7):1553-7. · 2.20 Impact Factor
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    ABSTRACT: Mycotoxins are fungal secondary metabolites that can be found in contaminated food and feed. There is some evidence to suggest that certain mycotoxins may be mutagenic. Here, we investigate the genotoxicity of the mycotoxin moniliformin (MON) (3-hydroxycyclobut-3-ene-1,2-dione) in human peripheral blood lymphocytes using chromosomal aberration (CA), sister-chromatid exchange (SCE), and micronucleus (MN) analysis. Lymphocyte cultures were treated for 48 h with six different concentrations of MON between 2.5 and 25 microM. CA, SCE, and MN frequencies were significantly increased in a dose-dependent manner compared with the negative control. The mitotic, replication, and cytokinesis-block proliferation indices were not affected by treatment with MON. The results provide evidence to demonstrate that MON can exert cytogenetic effects in human cells in culture.
    Environmental and Molecular Mutagenesis 03/2009; 50(5):431-4. · 3.71 Impact Factor
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    ABSTRACT: The genotoxic effects of fungicide Conan 5FL (containing 50 g/L hexaconazole) in mouse bone-marrow cells and human lymphocytes have been evaluated. Three different concentrations of Conan 5FL (17.50, 35.00 and 70.00 microg/mL for human lymphocytes and 17.50, 35.00 and 70.00 mg/kg for mouse bone marrow cells) were studied. Conan 5FL induced significant increases (except 17.50 mg/kg for mouse bone marrow) in the frequency of chromosomal aberrations (CAs) in both test systems. This fungicide caused structural and numerical abnormalities in both mammalian cells. These are sister chromatid union, chromatid and chromosome breaks, fragments, dicentric and ring chromosomes, and polyploidy. Significant increase was found in induction and in minimum-maximum numbers of sister chromatid exchanges (SCEs) at all treatments compared with the negative control. Conan 5FL did not affect the replication index (RI) in human lymphocyte cultures, however, it significantly decreased the mitotic index (MI) in all treatment concentrations in both test systems. Using of Conan 5FL should be reconsidered due to its possible cytotoxic, clastogenic and mutagenic effects.
    Genetika 04/2008; 44(3):323-8. · 0.37 Impact Factor
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    ABSTRACT: The genotoxic effects of the antifungal drug fluconazole (trade name triflucan) were assessed in the chromosome aberration (CA) test in mouse bone-marrow cells in vivo and in the chromosome aberration, sister chromatid exchange (SCE) and micronucleus (MN) tests in human lymphocytes. Fluconazole was used at concentrations of 12.5, 25.0 and 50.0 mg/kg for the in vivo assay and 12.5, 25.0 and 50.0 microg/ml were used for the in vitro assay. In both test systems, a negative and a positive control (MMC) were also included. Six types of structural aberration were observed: chromatid and chromosome breaks, sister chromatid union, chromatid exchange, fragments and dicentric chromosomes. Polyploidy was observed in both the in vivo and in vitro systems. In the in vivo test, fluconazole did not significantly increase the frequency of CA. In the in vitro assays, CA, SCE and MN frequencies were significantly increased in a dose-dependent manner compared with the negative control. The mitotic, replication and cytokinesis-block proliferation indices (CBPI) were not affected by treatments with fluconazole. According to these results, fluconazole is clastogenic and aneugenic in human lymphocytes, but these effects could not be observed in mice. Further studies should be conducted in other test systems to evaluate the full genotoxic potential of fluconazole.
    Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 02/2008; 649(1-2):155-60. · 3.90 Impact Factor
  • Fresenius Environmental Bulletin 01/2008; 17:1029-1037. · 0.64 Impact Factor
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    ABSTRACT: In the present study, chromosomal aberrations (CAs) and micronucleus (MN) levels in the lymphocytes from 60 male individuals consisting of 40 habitues of Maras powder (a kind of smokeless tobacco) and 20 unexposed subjects were determined to investigate the possible inducing effect of Maras powder. The consumers of Maras powder had no exposure to any other known mutagens or toxicants. The mean exposure period to Maras powder was 12.25 + 0.93 years (range 3-22). Data obtained from microscopic examination of the slides was analyzed by SPSS (10.0) package programme. Mean frequency of CA and MN was found to be significantly higher in Maras powder consumers as compared to controls. Similarly, there was a significant elevation in the level of aberrant cells (Ab.Cells) with CAs and binucleated cells with MN (BNMN) in habitues. Spearman's rho correlation analysis indicated a significant increase in the frequency of CA and MN with increase in both age and years of exposure in consumers. Our finding of a significant elevation of CA and MN frequencies in peripheral lymphocytes from smokeless tobacco consumers demonstrated a potential cytogenetic hazard associated with Maras powder exposure.
    Genetika 06/2007; 43(5):633-8. · 0.37 Impact Factor
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    ABSTRACT: The aim of this study was to investigate the genotoxic risk to workers occupationally exposed to coal combustion products in Afsin-Elbistan A power plant, located in south-eastern Turkey. We analysed chromosomal aberrations (CAs), polyploidy, sister-chromatid exchanges (SCEs), and micronuclei (MN) in 48 male workers without a history of smoking, tobacco chewing, or alcohol consumption. The results were compared with a control group of 30 healthy male individuals without exposure to any known genotoxic agents. The mean frequencies of CA, polyploidy, SCEs (P<0.01), and MN (P<0.05) were significantly higher in workers than in the control group, by the Mann-Whitney U-test. Spearman's rho correlation analysis revealed a significant increase in the frequency of CA and MN with increasing years of exposure (P<0.05). However, there was no significant effect of age on the cytogenetic markers analysed in both groups (P>0.05). The data obtained from this study clearly showed chromosomal hazard in the peripheral lymphocytes of workers exposed to coal combustion products in Afsin-Elbistan A power plant for several years. This cytogenetic damage might be attributed to the cumulative effects of several substances due to chemical complexity of the coal ash and gaseous emissions rather than a specific substance.
    Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 03/2007; 627(2):158-63. · 3.90 Impact Factor
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    ABSTRACT: In this investigation, clastogenic effects of Thymus kotschyanus var. glabrescens Boiss. extract (TE) and anticlastogenic effects of this extract against Mitomycin C (MMC) induced chromosome damage have been evaluated in human peripheral blood lymphocytes in vitro. Two series of experiments were conducted. In the first, only 10(-5), 10(-4), 10(-3) and 10(-2) mul ml(-1) concentrations of TE were used for 48 h to detect potential clastogenicity. In the second, MMC (0.38 mug ml(-1)) plus 10(-5), 10(-4), 10(-3) and 10(-2) mul ml(-1) concentrations of TE were used for 48 h to determine anticlastogenic effects. TE did not increase sister chromatid exchanges (SCEs) (except 10(-2) mul ml concentration) and chromosome aberrations (CAs) significantly compared with negative and solvent controls. However, it decreased the frequency of MMC induced chromosome aberrations. Decreasing was significant at 10(-4), 10(-3) and 10(-2) mul ml(-1) concentrations. On the other hand, TE significantly increased MMC-induced SCEs for all treatment groups compared with positive control.
    Cytotechnology 07/2006; 51(2):99-104. · 1.32 Impact Factor
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    ABSTRACT: The genotoxic effects of the fungicide afugan were analysed by measuring chromosomal aberrations (CAs), sister chromatid exchange (SCE) and micronuclei (MN) in cultured human peripheral lymphocytes. Concentrations of 2.5, 5, 10 and 20 microg/ml of afugan were used during 24 and 48 h. Afugan significantly increased the frequency of CAs at 5, 10 and 20 microg/ml concentrations during a 48 h treatment period. A significant increase was observed for induction of SCE and MN at all treatments compared with the negative control. A significant dose-response correlation was found in all tests. Afugan did not affect the replicative index (RI), however it significantly decreased the mitotic index (MI) at all treatment concentrations except 2.5 microg/ml, and at both treatment times. The present results indicate that afugan is clastogenic and cytotoxic to cultured human lymphocytes.
    Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 05/2006; 604(1-2):53-9. · 3.90 Impact Factor
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    ABSTRACT: The potential cytogenetic damage in offset printing workers was evaluated using sister chromatid exchanges (SCEs), chromosome aberrations (CAs) and micronuclei (MN) as biomarkers in peripheral lymphocytes of 26 volunteers (14 workers, 12 controls). The CA, SCE and MN frequency of offset printing workers was significantly higher than in their controls. The replication index (RI) was not affected while the mitotic index (MI) was affected most in the workers. It can be concluded from this study that chronic occupational exposure to printing dyes and thinner may lead to a slightly increased risk of genetic damage among offset printing workers.
    Journal of Applied Toxicology 01/2006; 26(1):10-5. · 2.60 Impact Factor
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    ABSTRACT: Karathane LC (active ingredient dinocap), a contact fungicide and a non-systemic acaricide was investigated for its ability to induce chromosome aberrations (CAs) and sister chromatid exchanges (SCEs) in cultured human lymphocytes of peripheral blood. In addition to the cytogenetic analysis, the effect of karathane LC on the cell proliferation kinetics (CPK) by the replication index (RI) was studied. The mitotic index (MI) was also determined to detect the cytotoxic effect. Lymphocytes were treated with four different concentrations (5, 10, 15 and 20 microg/ml) of karathane LC for 24 and 48 h. Significant differences between exposed and non-exposed groups found in CAs, SCEs and MI demonstrate the mutagenic, clastogenic and also the cytotoxic effect of karathane LC.
    Mutagenesis 04/2005; 20(2):101-4. · 3.50 Impact Factor
  • Cytologia - CYTOLOGIA TOKYO. 01/2005; 70(1):13-22.
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    ABSTRACT: The genotoxic effects of fungicide Conan 5FL (containing 50 g/L hexaconazole) in mouse bone-marrow cells and human lymphocytes have been evaluated. Three different concentrations of Conan 5FL (17.50, 35.0, and 70.0 μg/mL for human lymphocytes and 17.50, 35.0, and 70.0 mg/kg for mouse bone marrow cells) were studied. Conan 5FL induced significant increases (except 17.5 mg/kg for mouse bone marrow) in the frequency of chromosomal aberrations (CAs) in both test systems. This fungicide caused structural and numerical abnormalities in both mammalian cells. These are sister chromatid union, chromatid and chromosome breaks, fragments, dicentric and ring chromosomes, and polyploidy. Significant increase was found in induction and in minimum-maximum numbers of sister chromatid exchanges (SCEs) at all treatments compared with the negative control. Conan 5FL did not affect the replication index (RI) in human lymphocyte cultures, however, it significantly decreased the mitotic index (MI) in all treatment concentrations in both test systems. Using of Conan 5FL should be reconsidered due to its possible cytotoxic, clastogenic and mutagenic effects.
    Russian Journal of Genetics 44(3):273-278. · 0.43 Impact Factor

Publication Stats

63 Citations
109 Downloads
1k Views
32.40 Total Impact Points

Institutions

  • 2012
    • Ankara University
      • Faculty of Health Sciences
      Ankara, Ankara, Turkey
  • 2008–2012
    • Gazi University
      • Department of Biology
      Ankara, Ankara, Turkey
  • 2006–2012
    • Kahramanmaras Sutcu Imam University
      • • Faculty of Medicine
      • • Biology
      Marache, Kahramanmaraş, Turkey