Yongqiang Jiang

Publications (13)37.07 Total impact

• Article: DNA microarray analysis of acid-responsive genes of Streptococcus suis serotype 2

  Yan Wei, Xiaotao Zeng, Yuan Yuan, Hua Jiang, Yuling Zheng, Yafang Tan, Zhaobiao Guo, Ruifu Yang, Dongsheng Zhou, Yongqiang Jiang
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  ABSTRACT: An acidic environment is frequently found in phagocytes, while the intracellular survival and growth of Streptococcus suis serotype 2 (S. suis S2) in phagocytes is an essential part of the infection cycle of this pathogen. In this study, we used DNA microarrays to analyze the gene expression profile of S. suis S2 in response to acidic treatment. A total of 196 genes were differentially regulated when S. suis S2 was grown at pH5.8 relative to at pH7.2, especially including the inducible transcription of genes that encoded two-component regulatory systems, protection and repair functions, and intracellular pH homeostasis. The data showed that S. suis S2 is capable of employing diverse responsive mechanisms to protect against or adapt to acidic stress. Keywords Streptococcus suis serotype 2–Expression profile–Acid stress–Microarray
  Annals of Microbiology 04/2012; 61(3):505-510. · 0.69 Impact Factor
• Article: Identification and Cluster Analysis of Streptococcus pyogenes by MALDI-TOF Mass Spectrometry.

  Jie Wang, Na Zhou, Bin Xu, Huaijie Hao, Lin Kang, Yuling Zheng, Yongqiang Jiang, Hua Jiang
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  ABSTRACT: BACKGROUND: Whole-cell matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) has been successfully applied for bacterial identification and typing of many pathogens. The fast and reliable qualities of MALDI-TOF MS make it suitable for clinical diagnostics. MALDI-TOF MS for the identification and cluster analysis of Streptococcus pyogenes, however, has not been reported. The goal of our study was to evaluate this approach for the rapid identification and typing of S. pyogenes. METHODS: 65 S. pyogenes isolates were obtained from the hospital. The samples were prepared and MALDI-TOF MS measurements were conducted as previously reported. Identification of unknown spectra was performed via a pattern recognition algorithm with a reference spectra and a dendrogram was constructed using the statistical toolbox in Matlab 7.1 integrated in the MALDI Biotyper 2.0 software. RESULTS: For identification, 61 of 65 S. pyogenes isolates could be identified correctly by MALDI-TOF MS with BioType 2.0 when compared to biochemical identification (API Strep), with an accuracy of 93.85%. In clustering analysis, 44 of 65 isolates were in accordance with those established by M typing, with a matching rate of 67.69%. When only the M type prevalence in China was considered, 41 of 45 isolates were in agreement with M typing, with a matching rate of 91.1%. CONCLUSIONS: It was here shown that MALDI-TOF MS with Soft Biotype 2.0 and its database could facilitate rapid identification of S. pyogenes. It may present an attractive alternative to traditional biochemical methods of identification. However, for classification, more isolates and advances in the MALDI-TOF MS technology are needed to improve accuracy.
  PLoS ONE 01/2012; 7(11):e47152. · 4.09 Impact Factor
• Article: Microarray analysis of temperature-induced transcriptome of Streptococcus suis serotype 2.

  Xiaotao Zeng, Yuan Yuan, Yan Wei, Hua Jiang, Yuling Zheng, Zhaobiao Guo, Jiaqi Tang, Ruifu Yang, Dongsheng Zhou, Yongqiang Jiang
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  ABSTRACT: Streptococcus suis serotype 2 (S. suis S2) is able to cause human infections ranging from superficial wounded skin infections to severe invasive infections such as meningitis and streptococcal toxic shock-like syndrome. During its infection cycle, S. suis S2 must acclimatize itself to temperature shift. Herein, a whole-genome DNA microarray was used to investigate the global transcriptional regulation of an invasive strain of S. suis S2 grown to late-exponential phase at 29°C or 40°C relative to 37°C. The differentially regulated genes that were detected included those encoding virulence factors, antigenic proteins, ATP-binding-cassette transporters, and proteins of unknown functions. Our data provided a global profile of gene transcription induced by temperature alteration and shed light on some unforeseen lines for further pathogenesis investigation.
  Vector borne and zoonotic diseases (Larchmont, N.Y.) 03/2011; 11(3):215-21. · 2.61 Impact Factor
• Source

  Available from: PubMed Central

  Article: Probing genomic diversity and evolution of Streptococcus suis serotype 2 by NimbleGen tiling arrays.

  Zuowei Wu, Ming Li, Changjun Wang, Jing Li, Na Lu, Ruifen Zhang, Yongqiang Jiang, Ruifu Yang, Cuihua Liu, Hui Liao, George F Gao, Jiaqi Tang, Baoli Zhu
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  ABSTRACT: Our previous studies revealed that a new disease form of streptococcal toxic shock syndrome (STSS) is associated with specific Streptococcus suis serotype 2 (SS2) strains. To achieve a better understanding of the pathogenicity and evolution of SS2 at the whole-genome level, comparative genomic analysis of 18 SS2 strains, selected on the basis of virulence and geographic origin, was performed using NimbleGen tiling arrays. Our results demonstrate that SS2 isolates have highly divergent genomes. The 89K pathogenicity island (PAI), which has been previously recognized as unique to the Chinese epidemic strains causing STSS, was partially included in some other virulent and avirulent strains. The ABC-type transport systems, encoded by 89K, were hypothesized to greatly contribute to the catastrophic features of STSS. Moreover, we identified many polymorphisms in genes encoding candidate or known virulence factors, such as PlcR, lipase, sortases, the pilus-associated proteins, and the response regulator RevS and CtsR. On the basis of analysis of regions of differences (RDs) across the entire genome for the 18 selected SS2 strains, a model of microevolution for these strains is proposed, which provides clues into Streptococcus pathogenicity and evolution. Our deep comparative genomic analysis of the 89K PAI present in the genome of SS2 strains revealed details into how some virulent strains acquired genes that may contribute to STSS, which may lead to better environmental monitoring of epidemic SS2 strains.
  BMC Genomics 01/2011; 12:219. · 4.07 Impact Factor
• Article: Global analysis of a plasmid-cured Shigella flexneri strain: new insights into the interaction between the chromosome and a virulence plasmid.

  Li Zhu, Xiankai Liu, Xuexue Zheng, Xin Bu, Ge Zhao, Chaohua Xie, Jingfei Zhang, Na Li, Erling Feng, Jie Wang, Yongqiang Jiang, Peitang Huang, Hengliang Wang
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  ABSTRACT: Shigella flexneri is an important human pathogen that causes dysentery, and remains a significant threat to public health, particularly in developing countries. The virulence of this pathogen is dependent on an acquired virulence plasmid. To investigate the crosstalk between the bacterial chromosome and the exogenous virulence plasmid, a virulence plasmid-cured strain was constructed using plasmid incompatibility. The global patterns of gene expression of this strain compared with the wild-type strain were analyzed using 2-DE combined with MALDI-TOF MS. Most known virulence factors of S. flexneri were identified in the 2-DE gels. Interestingly, the expression of the glycerol 3-phosphate (glp) regulon-encoded proteins was increased when the virulence plasmid was absent. Microarray analysis confirmed that regulation occurred at the transcriptional level. Purification and identification of DNA binding proteins with affinity for the regulatory region of the glp genes revealed that regulation mediated by the virulence plasmid to control the expression of the glp regulon might in turn be mediated by protein GlpR. To our knowledge, this is the first study analyzing the interaction between a pathogen chromosome and a virulence plasmid at the proteomic level.
  Journal of Proteome Research 12/2009; 9(2):843-54. · 5.11 Impact Factor
• Article: Simultaneous detection of five biothreat agents in powder samples by a multiplexed suspension array.

  Jing Wang, Yu Yang, Lei Zhou, Jinglin Wang, Yongqiang Jiang, Kongxin Hu, Xiaohong Sun, Yousong Hou, Ziwen Zhu, Zhaobiao Guo, Yanli Ding, Ruifu Yang
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  ABSTRACT: A suspension array-based multiplexed immunoassay was developed for rapid, sensitive, specific, and simultaneous detection of multiple biothreat-associated agents in powder samples. The 5-plexed immunoassays using sets of 9-plexed coupled fluorescent beads were employed to simultaneously detect five representative biothreat agents, including B. anthracis spore, Y. pestis, SARS-CoV, staphylococcal enterotoxin B (SEB) and ricin from a single powder sample and the feasibility for field samples was demonstrated by both blinded and standard laboratory trials. The detection sensitivity and dynamic range for the five biothreat agents from different powders might be varied depending on the nature of the powder and the feature of the contaminating agent. The limit of detection for Y. pestis, B. anthracis spores, SEB, ricin, SARS-CoV N protein in milk powder was 20 cfu, 111 cfu, 110pg, 5.4 ng and 2 ng per test respectively. Compared to conventional ELISA method, the suspension array has a higher sensitive ability, and can detect five biothreat agents simultaneously with high reproducibility.
  Immunopharmacology and Immunotoxicology 05/2009; 31(3):417-27. · 1.83 Impact Factor
• Source

  Available from: Ruifu Yang

  Article: Genomic research for important pathogenic bacteria in China.

  RuiFu Yang, XiaoKui Guo, Jian Yang, YongQiang Jiang, Bo Pang, Chen Chen, YuFeng Yao, JinHong Qin, QingTian Li
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  ABSTRACT: Rapid accumulation of bacterial genomic data offered an unprecedented opportunity to understand bacterial biology from a holistic view of point. We can thus closely look at the way in which a pathogen is evolved, and these data has been applied to molecular epidemiology and microbial forensics, and screening of novel diagnostic, vaccine and drug targets. The newly developed high-throughput low-cost sequencing technologies, such as 454, Solexa and SOLiD, will promote the acquisition and application of genomic data in new research areas that we dared not imagine previously, such as the metagenomics of human gastric-intestinal tract, for better and comprehensive understanding of human health and disease.
  Science in China Series C Life Sciences 02/2009; 52(1):50-63. · 1.61 Impact Factor
• Article: Identification and characterization of novel immunogenic proteins of Streptococcus suis serotype 2.

  Hongran Geng, Li Zhu, Yuan Yuan, Wei Zhang, Wenjun Li, Jie Wang, Yuling Zheng, Kaihua Wei, Wuchun Cao, Hengliang Wang, Yongqiang Jiang
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  ABSTRACT: Streptococcus suis, a zoonotic pathogen, caused serious outbreaks in humans with high mortality rates in the past decade. To develop safer and more effective vaccines, particularly for human protection, cell wall and extracellular proteins of S. suis serotype 2 were analyzed by an immunoproteomic approach in this study. Thirty-two proteins with high immunogenicity were identified and 22 of them were newly identified. Further analyses of 9 selected proteins revealed that (1) these 9 proteins were expressed in all tested virulent S. suis serotype 2 isolates, (2) antisera against 6 of the selected proteins efficiently killed the bacteria by opsonized phagocytosis in human blood, and (3) significantly higher levels of serum antibodies against 3 proteins were detected in both patients and infected swines. Therefore, our results suggest the 3 proteins (SSU98_0197, SSU98_1094 and SSU1664) have strong potential to be vaccine candidates.
  Journal of Proteome Research 09/2008; 7(9):4132-42. · 5.11 Impact Factor
• Article: Targeting matrix metalloproteinases and endothelial cells with a fusion peptide against tumor.

  Yufei Zou, Yahui Chen, Yongqiang Jiang, Jin Gao, Jun Gu
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  ABSTRACT: Development of novel therapy for patients with tumor is still a challenge at the present time. We designed a fusion peptide (RK5) with two targets as a novel agent against tumor. The fusion peptide RK5 containing the kringle 5 fragment of human plasminogen and a decapeptide (CTTHWGFTLC) was constructed and expressed in yeast. Matrix metalloproteinase (MMP) activity, proliferation, and migration of endothelial cells were examined in vitro, respectively. Angiogenesis, tumor growth, metastasis, and survival time were evaluated in in vivo models. Administration of RK5 was delivered by both protein and gene approach. The results showed that RK5 inhibited the activity of MMP-9 and exhibited more inhibitory effects on proliferation and migration of endothelial cells than that of kringle 5 fragment and decapeptide individually. RK5 also inhibited angiogenesis, tumor growth, and metastasis and increased survival time of mice bearing tumor. In addition, the effectiveness of RK5 could be achieved by both protein and gene delivery. In conclusion, RK5 has potential to inhibit tumor growth and metastasis and to prolong survival time of animals bearing tumor. Therefore, fusion peptide RK5 with two targets provides a new design for the development of antitumor drugs and has potential for clinical application.
  Cancer Research 09/2007; 67(15):7295-300. · 7.86 Impact Factor
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  Available from: Yajun Song

  Article: A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates.

  Chen Chen, Jiaqi Tang, Wei Dong, Changjun Wang, Youjun Feng, Jing Wang, Feng Zheng, Xiuzhen Pan, Di Liu, Ming Li, [......], Wen Sun, Yongqiang Jiang, Jun Wang, Jinghua Yan, Huanming Yang, Xiaoning Wang, George F Gao, Ruifu Yang, Jian Wang, Jun Yu
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  ABSTRACT: Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen, causing more than 200 cases of severe human infection worldwide, with the hallmarks of meningitis, septicemia, arthritis, etc. Very recently, SS2 has been recognized as an etiological agent for streptococcal toxic shock syndrome (STSS), which was originally associated with Streptococcus pyogenes (GAS) in Streptococci. However, the molecular mechanisms underlying STSS are poorly understood. To elucidate the genetic determinants of STSS caused by SS2, whole genome sequencing of 3 different Chinese SS2 strains was undertaken. Comparative genomics accompanied by several lines of experiments, including experimental animal infection, PCR assay, and expression analysis, were utilized to further dissect a candidate pathogenicity island (PAI). Here we show, for the first time, a novel molecular insight into Chinese isolates of highly invasive SS2, which caused two large-scale human STSS outbreaks in China. A candidate PAI of approximately 89 kb in length, which is designated 89K and specific for Chinese SS2 virulent isolates, was investigated at the genomic level. It shares the universal properties of PAIs such as distinct GC content, consistent with its pivotal role in STSS and high virulence. To our knowledge, this is the first PAI candidate from S. suis worldwide. Our finding thus sheds light on STSS triggered by SS2 at the genomic level, facilitates further understanding of its pathogenesis and points to directions of development on some effective strategies to combat highly pathogenic SS2 infections.
  PLoS ONE 02/2007; 2(3):e315. · 4.09 Impact Factor
• Article: Second-harmonic generation investigations of Zn_ {1-x} Cd_ {x} Se/ZnSe asymmetric coupled quantum wells

  Yongqiang Jiang, Jianhua Xu, Wenjun Wang, Xingze Lu, Xiu Liu, Gongming Wang, Fuming Li
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  ABSTRACT: Second-order nonlinear optical properties of strained Zn1-xCdxSe/ZnSe asymmetric coupled quantum wells (ACQW’s) have been investigated by using the reflective second-harmonic generation (SHG) technique. Compared with the SHG intensities in ZnSe bulk material, a significant enhancement of the signals was observed in ACQW’s due to the centrosymmetry-breaking effect resulting from the strong-coupling interaction between adjacent quantum wells. The second-harmonic intensity was also found to increase with decreasing cadmium concentration x because of the second-harmonic off-resonance effect. Moreover, the SHG intensity versus the sample azimuthal angle shows an obvious in-plane anisotropy with a period of 180°, and our numerical calculations of the SHG dependence on azimuthal or polarization angle agreed well with experimental results.
  Phys. Rev. B. 63(12).
• Source

  Available from: chen-server.mer.utexas.edu

  Article: Thermally tuned optical fiber for true time delay generation

  Brie Howley, Zhong Shi, Yongqiang Jiang, Ray T. Chen
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  ABSTRACT: A new technique for generating a continuous range of true time delay values is introduced. Heating optical fiber in order to change the effective index of the guided mode produces time delays. A 45-m section of single-mode silica fiber is demonstrated to produce a continuous range of time delay values from 0 to over a temperature tuning range of 50°C (30–80°C). A thermal time delay factor is introduced and found to be for Corning LEAF fiber. A 7.66-m section of multimode Lucina polymer fiber is demonstrated to produce a range of time delay values from 0 to over a temperature tuning range of 30°C (30–60°C). The thermal time delay factor for this fiber is .
  Optics & Laser Technology.
• Article: Second harmonic generation investigation of indium tin oxide thin films

  Wenjun Wang, Jianhua Xu, Xiu Liu, Yongqiang Jiang, Gongming Wang, Xingze Lu
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  ABSTRACT: Second harmonic generation (SHG) from indium tin oxide (ITO) films on glass substrates has been investigated in detail for the first time. Significant SHG intensity and non-linear optical anisotropy was observed from ITO films due to orientation of the crystal cells. The SHG reached a maximum in intensity at an incident angle of 60°. The effective second order non-linear optical susceptibility χ2eff was about 2.4×10−10 esu.
  Thin Solid Films.