-
[show abstract]
[hide abstract]
ABSTRACT: In the present study, an ultra performance liquid chromatography coupled with photodiode array detector and time-of-flight mass spectrometry (UPLC-PDA-TOF/MS) was proposed and validated for rapidly analyzing and evaluating Ginkgo biloba leaves from different origins by using multivariate statistical analysis. Batches of these kinds of G. biloba leaves were subjected to UPLC-PDA-TOF/MS analysis, the datasets of retention time (RT)-m/z pairs, ion intensities and sample codes were further processed with orthogonal partial least squared discriminant analysis (OPLS-DA) to holistically compare the difference between these G. biloba leaves, and to generate an S-plot. Those compounds correlated to the points at the two ends of S were regarded as the most differentiating components. By comparing the mass/UV spectra and retention times with those of reference compounds and/or tentatively assigned by matching empirical molecular formulae with those of the known compounds published in the literatures, these differentiating components were finally characterized as kaempferol 3-O-[2-O-(6-O-p-hydroxy-trans-cinnamoyl)-β-D-glucosyl)-α-L-rhamnoside], kaempferol 3-O-[2-O,6-O-bis(α-L-rhamnosyl)-β-D-glucoside], ginkgolide C, kaempferol 3-O-[2-O-(β-D-glucosyl)-α-L-rhamnoside], and bilobetin. These compounds would be the potential chemical markers for the two kinds of leaves. The results suggested that this newly established approach could be used to rapidly evaluate the quality of herbs from different origin, and to provide good strategy for further rectify and standardize the herb market. Copyright © 2013 John Wiley & Sons, Ltd.
Drug Testing and Analysis 05/2013; · 2.54 Impact Factor
-
Chemical Reviews 03/2013; · 40.20 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: In this study, a sensitive and rapid method for the simultaneous determination of free amino acids without derivatization using hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry (HILIC-MS/MS) was developed. The method was performed on an ultra-high-performance liquid chromatography (UHPLC) separation system coupled with a triple-quadrupole mass spectrometry (TQ-MS) instrument. Sufficient separation of 23 underivatized amino acids was achieved on an Acquity BEH Amide column (2.1 mm × 100 mm, 1.7 μm) in a single run of 12 min. Then the method was applied for the analysis of the free amino acids in 46 batches of Ziziphus jujuba fruits which comprised 39 cultivars from 26 cultivation regions. Multivariate statistical analysis was also used to investigate the differences in free amino acid profiles among the samples. This study showed that HILIC-UHPLC-TQ-MS is an effective technique to analyze underivatized amino acids in the food samples.
Journal of Agricultural and Food Chemistry 03/2013; · 2.82 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: BACKGROUND: Siwu decoction categorized formulae (SWDCF) are widely used for treating gynecological diseases. This study aims to elucidate the differences of bioactive constituents in SWDCF by ultra-high performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC - QTOF - MS /MS) and HPLC-DAD. METHODS: An efficient method based on UPLC - QTOF - MS /MS was developed for identifying the chemical profiles of SWDCF. HPLC-DAD method was used for quantifying seven chemical markers in SWDCF. RESULTS: Eighty four components were identified or characterized, including ten organic acids, thirty glycosides (monoterpene or iridoid or phenylpropanoids glycosides), fourteen lactones, eighteen flavonoids, and eleven alkaloids in the complex system. The datasets of tR-m/z pairs, ion intensities and sample codes were processed with supervised orthogonal partial least squared discriminant analysis to compare these decoction samples. After a clear classification was established, OPLS-DA was performed and 16 common components with relative quantity in SWDCF samples were determined. Gallic acid, protocatechuic acid, vanillic acid, caffeic acid, paeoniflorin, ferulic acid, and senkyunolide I were selected as the chemical markers to identify SWDCF by HPLC-DAD. CONCLUSION: The chemical profiles with 84 components in SWDCF, including monoterpene glycosides, acetophenones, galloyl glucoses, even some isomers in the complex system were characterized by UPLC--QTOF--MS/MS.
Chinese Medicine 03/2013; 8(1):5. · 1.79 Impact Factor
-
Guisheng Zhou,
Hanqing Pang, Yuping Tang,
Xin Yao,
Xuan Mo,
Shaoqing Zhu,
Sheng Guo,
Dawei Qian,
Yefei Qian,
Shulan Su,
Li Zhang,
Chun Jin,
Yong Qin,
Jin-Ao Duan
[show abstract]
[hide abstract]
ABSTRACT: This work presented a new analytical methodology based on hydrophilic interaction ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry in multiple-reaction monitoring mode for analysis of 24 underivatized free amino acids (FAAs) in functional foods. The proposed method was first reported and validated by assessing the matrix effects, linearity, limit of detections and limit of quantifications, precision, repeatability, stability and recovery of all target compounds, and it was used to determine the nutritional substances of FAAs in ginkgo seeds and further elucidate the nutritional value of this functional food. The result showed that ginkgo seed turned out to be a good source of FAAs with high levels of several essential FAAs and to have a good nutritional value. Furthermore, the principal component analysis was performed to classify the ginkgo seed samples on the basis of 24 FAAs. As a result, the samples could be mainly clustered into three groups, which were similar to areas classification. Overall, the presented method would be useful for the investigation of amino acids in edible plants and agricultural products.
Amino Acids 01/2013; · 3.25 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Primary dysmenorrhea (PD) is characterized by painful menstrual cramps without any organic pathology and has a prevalence of up to 90% in the adolescents. Recent advances in its etiology and pathogenesis are providing more speculative hypotheses focused on integral systems. Using a targeted tandem mass spectrometry (MS/MS) - based metabolomic platform, we explored the changes of metabolic profiling in plasma / urine simultaneously between PD patients and healthy controls before and after a 3- month herbal medicine (namely Shaofu Zhuyu formula concentrated - granule, SFZYFG) therapy. To detect and identify potential biomarkers associated with PD and SFZYFG treatment, we also performed a combined UPLC-QTOF-MS/MS based metabolomic profiling of the plasma/urine samples, indicating a further deviation of the patients' global metabolic profile from that of controls. The total 35 metabolites (19 in plasma and 16 in urine), up regulated or down regulated (p 0.05 or 0.01), were identified and contributed to PD progress. These promising identified biomarkers underpin the metabolic pathway including sphingolipid metabolism, steroid hormone biosynthesis, and glycerophospholipid metabolism are disturbed in PD patients, which were identified by using pathway analysis with MetPA. 24 altered metabolites and 14 biochemical indicators were restored back to the control- like level after the treatment of SFZYFG and could be potential biomarkers for monitoring therapeutic efficacy. These findings may be promise to yield a valuable insight into the pathophysiology of PD and advance the approaches of treatment, diagnosis and prevention of PD and related syndromes.
Journal of Proteome Research 01/2013; · 5.11 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: A sensitive and accurate ultra-performance liquid chromatography coupled with triple quadrupole mass (UPLC-MS/MS) method was developed for the determination of quercetin-3-O-β-D-glucopyranoside-(4→1)-α-L-rhamnoside (QGR) in rat plasma using rutin as internal standard. Chromatographic separation was achieved on a Acquity BEH C column (100 mm × 2.1 mm, 1.7 μm) with a gradient elution of acetonitrile and 0.10% formic acid (v/v) at a flow rate of 0.4 mL/min. QGR and rutin were detected using electrospray negative ionization mass spectrometry in the multiple reaction monitoring (MRM) mode. The method demonstrated good linearity and did not show any endogenous interference with the QGR and rutin peaks. This method was successfully applied to a pharmacokinetic study of QGR in rats after intravenous (20 mg/kg) and oral (40 mg/kg) administration, and the results showed that the compound was poorly absorbed, with an absolute bioavailability of approximately 3.41%.
Molecules 01/2013; 18(3):3050-9. · 2.39 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Primary dysmenorrhea is one of the most common gynecological complaints in young women, but potential peripheral immunologic features underlying this condition remain undefined. In this paper, we compared 84 common cytokine gene expression profiles of peripheral blood mononuclear cells (PBMCs) from six primary dysmenorrheic young women and three unaffected controls on the seventh day before (secretory phase), and the first (menstrual phase) and the fifth (regenerative phase) days of menstruation, using a real-time PCR array assay combined with pattern recognition and gene function annotation methods. Comparisons between dysmenorrhea and normal control groups identified 11 (nine increased and two decreased), 14 (five increased and nine decreased), and 15 (seven increased and eight decreased) genes with ≥2-fold difference in expression (P<0.05) in the three phases of menstruation, respectively. In the menstrual phase, genes encoding pro-inflammatory cytokines (IL1B, TNF, IL6, and IL8) were up-regulated, and genes encoding TGF-β superfamily members (BMP4, BMP6, GDF5, GDF11, LEFTY2, NODAL, and MSTN) were down-regulated. Functional annotation revealed an excessive inflammatory response and insufficient TGF-β superfamily member signals with anti-inflammatory consequences, which may directly contribute to menstrual pain. In the secretory and regenerative phases, increased expression of pro-inflammatory cytokines and decreased expression of growth factors were also observed. These factors may be involved in the regulation of decidualization, endometrium breakdown and repair, and indirectly exacerbate primary dysmenorrhea. This first study of cytokine gene expression profiles in PBMCs from young primary dysmenorrheic women demonstrates a shift in the balance between expression patterns of pro-inflammatory cytokines and TGF-β superfamily members across the whole menstrual cycle, underlying the peripheral immunologic features of primary dysmenorrhea.
PLoS ONE 01/2013; 8(2):e55200. · 4.09 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: A rapid and sensitive ultra performance liquid chromatography-tandem mass spectrometry method was developed for simultaneous determination of eight constituents, uralsaponin C, uralsaponin F, 22β-acetoxyl-glycyrrhizin, 24-hydroxy-licorice-saponin E2, licorice-saponin G2, licorice-saponin E2, glycyrrhizin, and licorice-saponin J2, from total saponin of licorice (TSL) in dog plasma. Ardisiacrispin A was used as the internal standard (IS). The separation was performed on Thermo Syncronic C(18) column (100mm×2.1mm, 1.7μm) at a flow rate of 0.4mLmin(-1), and acetonitrile/methanol (3:1, v/v)-0.1% formic acid was used as mobile phase. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) via electrospray ionization (ESI) source with negative ionization mode. All calibration curves had good linearity (r>0.991) over the concentration range from 2.03-405ngmL(-1) to 2.63-2625ngmL(-1) for all components. The intra- and inter-day precision was within 15% and the accuracy ranged from -14.08% to 13.8%. The method was successfully applied to pharmacokinetic study of eight triterpenoid saponins in dog plasma after oral administration of TSL.
Journal of pharmaceutical and biomedical analysis 12/2012; 75C:248-255. · 2.45 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: BACKGROUND: In the present study, chemical consistency between traditional and dispensing granule decoctions of Tao-Hong-Si-Wu decoction was rapidly evaluated by UPLC-QTOF-MS coupled with the MarkerLynx software. Two different kinds of decoctions, namely traditional decoction: water extract of mixed six constituent herbs of Tao-Hong-Si-Wu decoction, and dispensing granules decoction: mixed water extract of each individual herbs of Tao-Hong-Si-Wu decoction, were prepared. RESULTS: Chemical difference was found between traditional and dispensing granule decoctions, and albiflorin, paeoniflorin, gallic acid, amygdalin, and hydroxysafflor yellow A were identified as the significantly changed components during decocting Tao-Hong-Si-Wu decoction. All the peaks of mass spectrum from Tao-Hong-Si-Wu decoction and each herb were extracted and integration by using QuanLynxTM. And the optimized data was used for linear regression analysis. The contribution of each herb in Tao-Hong-Si-Wu decoction, and the optimal compatibility proportion of dispensing granule decoction were derived from the linear regression equation. CONCLUSIONS: The optimal dosage proportionality of Tao-Hong-Si-Wu dispensing granule decoction was obtained as 2.5:0.2:1:0.5:0.6:0.1 (DG : CX : BS : SD : TR : HH), which guided better clinic application of Tao-Hong-Si-Wu decoction as dispensing granule decoctions usage, and it also provided some experimental data to reveal the compatibility rule of the relative TCM formulae.
Chemistry Central Journal 11/2012; 6(1):143. · 3.28 Impact Factor
-
Meijuan Chen,
Yuhong Du,
Min Qui,
Mingyan Wang,
Kejun Chen,
Zhenzhou Huang,
Miao Jiang,
Fei Xiong,
Jianping Chen,
Jing Zhou,
Fengrong Jiang,
Lian Yin, Yuping Tang,
Lihong Ye,
Zhen Zhan,
Jin-Αo Duan,
Hai-Αn Fu,
Xu Zhang
[show abstract]
[hide abstract]
ABSTRACT: Ophiopogonin B (OP-B) is a bioactive component of Radix Ophiopogon Japonicus, which is often used in Chinese traditional medicine to treat pulmonary disease. However, whether or not OP-B has any potential antitumor activity has not been reported. Here, we show that the non-small cell lung cancer (NSCLC) cell lines NCI-H157 and NCI-H460 treated with OP-B grow more slowly and accumulate vacuoles in their cytoplasm compared to untreated control cells. Flow cytometric analysis showed that the cells were arrested in G0/G1 phase. Nuclear morphology, Annexin-V/PI staining, and expression of cleaved caspase-3 all confirm that OP-B does not induce apoptosis. Instead, based on results from both transmission electron microscopy (TEM) and the expression of microtubule-associated protein 1 light chain 3-II (LC3-II), we determined that OP-B treatment induced autophagy in both cell lines. Next, we examined the PI3K/Akt/mTOR signaling pathway and found that OP-B inhibited phosphorylation of Akt (Ser473, Thr308) in NCI-H157 cells and also inhibited several key components of the pathway in NCI-H460 cells, such as p-Akt(Ser473, Thr308), p-p70S6K (Thr389). Additionally, insulin-mediated activation of the PI3K/Akt/mTOR pathway provides evidence that activation of this pathway may correlate with induction of autophagy in H460 cells. Therefore, OP-B is a prospective inhibitor of PI3K/Akt and may be used as an alternative compound to treat NSCLC.
Oncology Reports 11/2012; · 1.84 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Primary dysmenorrhea (PDM), a common clinical endocrine disorder affecting young women, is associated with endocrinopathy and metabolic abnormalities. Although some physiological and pathological function parameters have been investigated, little information about the changes of small metabolites in biofluids has been reported, which may cause poor diagnosis and treatment for PDM. The Xiang-Fu-Si-Wu Formula (XFSWF) is a Chinese herbal formula used to treat PDM for hundreds of years. The aim of this study was to establish the metabolic profile of PDM and investigate the action mechanism of XFSWF effect. In this cross-sectional study of 25 patients with PDM and 12 healthy controls, contents of small molecular endogenous metabolites in blood plasma and urine samples were measured by ultra performance liquid chromatography (UPLC) coupled with quadrupole-time-of-flight mass spectrometry (QTOF/MS) and triple quadrupole mass spectrometry (QqQ/MS) based techniques and analyzed by multivariate statistical methods. The levels of LPCs including lypso (16 : 1), lysoPC(20 : 4), lysoPC(18 : 2), lysoPC(16 : 0), lysoPC(18 : 1), lysoPC(10 : 1), estrone, 17-hydroxyprogesterone, myristoylglycine and palmitoylglycine increased significantly (p < 0.05) in PDM, while the levels of phytosphingosine, dihydrocortisol and sphingosine decreased significantly (p < 0.05) compared with the healthy controls. These significant perturbations are involved in glycerophospholipid metabolism and sphingolipid metabolism, as well as steroid hormone biosynthesis. The metabolic deviations recovered to the normal level after XFSWF intervention. The results demonstrated that biofluids metabonomics was a powerful tool in clinical diagnosis and treatment of PDM for providing information on changes in metabolites and neural, endocrinal and immune pathways. XFSWF can be used for the treatment of PDM cases, especially for those adolescents who do not desire a contraceptive method, to reduce the risk of secondary dysmenorrhea.
Molecular BioSystems 10/2012; · 3.53 Impact Factor
-
Guisheng Zhou,
Xin Yao, Yuping Tang,
Nianyun Yang,
Hanqing Pang,
Xuan Mo,
Shaoqing Zhu,
Shulan Su,
Dawei Qian,
Chun Jin,
Yong Qin,
Jin-Ao Duan
[show abstract]
[hide abstract]
ABSTRACT: Two new fatty alcohols named as (7S,8R,11S)-nonacosanetriol (1) and (10R,12R,15S)-nonacosanetriol (2), along with eight known compounds including ginkgolic acid (3), hydroginkgolic acid (4), sciadopitysin (5), ginkgetin (6), isoginkgetin (7), ginkgolide A (8), ginkgolide B (9) and ginkgolide C (10) have been isolated from the petroleum ether extract of Ginkgo biloba sarcotesta. Their structures were elucidated by means of chemical and extensive spectroscopic analysis. The absolute stereochemistry of compounds 1 and 2 was elucidated on the spectroscopic analysis of the R- and S-MTPA esters. Compounds 1 and 2 exhibited slight activity of antithrombin and moderate activity of antiplatelet aggregation in vitro. This was the first report regarding the anticoagulative activities of biflavonoids in G. biloba, and isoginkgetin (7) showed significant antithrombin and antiplatelet aggregation activity.
Chemistry and physics of lipids 09/2012; 165(7):731-736. · 2.15 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: The anticancer activities of alkyl esters and NO-donors of ferulic acid (FA) and caffeic acid (CA) were assessed by a high-throughout screening (HTS) method, and the structure-activity relationships were described. CA alkyl esters had better anticancer activities than FA alkyl esters with the same alkyl substituent. Mono-nitrates and phenylfuroxan nitrates were more potent than the dual nitrates. Phenylsulfonylfuroxan nitrates of FA, especially compounds 8b-8d, exhibited more potent activities in anticancer.
Bioorganic & medicinal chemistry letters 08/2012; 22(19):6085-8. · 2.65 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To compare the toxicity on normal liver cells LO2 before and after Kansui Radix stir-baked with vinegar, and make a preliminary study on the mechanism of detoxication of Kansui Radix stir-baked with vinegar.
The MTT method was adopted to detect the cell activity, with normal liver cells LO2 as the study object. The morphology of cells were observed, and the level or content of AST, ALT, LDH, SOD, Na+-K+-ATPase, Ca2+-Mg2+ -ATPase, GSH and MDA were determined in cell culture supernatant and splitting supernatant.
Compared with the control group, Kansui can obviously inhibit the cell activity (P < 0.01) and morphology, and increase the levels of ALT, AST, and LDH (P < 0.01) in the supernatant fluid of cell incubation, and decrease the level of SOD and the content of GSH (P < 0.01). Besides, it significantly increased the content of MDA (P < 0.01) and significantly decreased the level of Na+-K+-ATPase and Ca2+-Mg2+ -ATPase (P < 0.01) in the supernatant fluid of cell dissociation. Compared with Kansui group of various doses, Kansui Radix stir-baked with vinegar can significantly decrease the cell proliferation inhibition and the trend of morphological variation, and obviously decrease the levels of ALT, AST, and LDH (P < 0.01) in the supernatant fluid of cell incubation, and significantly increase the level of SOD and the content of GSH (P < 0.01), and significantly decrease the content of MDA (P < 0.01). Additionally, it significantly increased the level of Na+-K+-ATPase and Ca2+-Mg2+ ATPase (P < 0.01) in the supernatant fluid of cell dissociation, and showed a certain dose-effect relationship.
Stir-baking with rice vinegar can release the hepatotoxicity of Kansui Radix. Its possible mechanism was that Kansui Radix stir-baked with vinegar can decrease the influence of Kansui Radix on the permeability of liver cells LO2 membrane and oxidative damage, in order to provide basis for further exploration of the detoxication mechanism of Kansui Radix stir-baked with vinegar.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 06/2012; 37(11):1667-71.
-
[show abstract]
[hide abstract]
ABSTRACT: In China the roots of Euphorbia kansui T.N. Liou ex T.P. Wang, known as ‘kansui’, have been used for centuries as a herbal remedy for edema, ascites, and asthma.
Kansui, has inflammatory and tumor-promoting toxicity, and other side-effects, however, which have seriously restricted its
clinical application. In the work discussed in this paper a simple and rapid LC–DAD–ESI-MS–MS method has been established
for separation and characterization of the main compounds in the toxic fraction of E. kansui roots. Twelve diterpene derivatives were identified in the inflammatory fraction of kansui: kansuinine C, kansuinine B, kansuinine
A, kansuinine D, 5-O-benzoyl-20-deoxyingenol, kansuinine E, kansuiphorin C, 3-O-benzoyl-20-deoxyingenol, 3-O-(2′E,4′Z-decadienoyl)-5-O-acetylingenol, 3-O-(2′E,4′Z-decadienoyl)-20-deoxyingenol, 20-O-(2′E, 4′E-decadienoyl)ingenol, and 3-O-(2′E,4′Z-decadienoyl)-20-O-acetylingenol. The inflammatory fraction was separated on a C18 reversed-phase column with a mobile phase gradient. The proposed method is a scientific and technical platform enabling the
herbal medicine industry to perform quality control and ensure the safety of preparations that contain this class of poisonous
diterpenoids.
Chromatographia 04/2012; 70(5):805-810. · 1.20 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: In this paper, ultra performance liquid chromatography (UPLC)–quadrupole-time-of-flight mass spectrometry (QTOF) with collision
energy (MS
E
) and MassFragment software were applied for fast separation and characterization of constituents in HuangKui Capsules (HKC).
MS
E
was used for simultaneous acquisition of precursor ion information and fragment ion data at high and low collision energy
in one analytical run, which facilitated the fast structural characterization of 12 constituents in HKC. When the reference
compound was not available, MassFragment software was used to confirm the proposed structure, which increased our confidence
in the MS fragment analysis of the proposed structure, facilitating the confirmation of the proposed structure and therefore
easing the data processing procedure. In conclusion, UPLC–QTOF-MS
E
and MassFragment software described in this paper proved to be useful for the identification of constituents in complex herbal
medicines and could greatly decrease analytical time. In addition, this method may be applied to the analysis of more complex
data found in Traditional Chinese Medicine.
KeywordsUPLC–QTOF-MS–MassFragment software–HuangKui Capsules
Chromatographia 04/2012; 73(5):447-456. · 1.20 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To study the dissolution rate of active components of different extracting solvents of Danggui Chishao drug pair.
The dissolution rates of phenolic acids (ferulic acid, vanillic acid and gallic acid), monoterpenes (gallic acid, peoniflorin, albiflorin, hydroxypeoniflorin and galloylpaeoniflorin) and phthalates (senkyunolide and ligustilide) contained in Danggui Chishao drug pair were determined by quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS) and ultra high performance liquid chromatography in combination with triple-quadrupole mass spectrometry (UPLC-TQ-MS).
The dissolution rates of phenolic acids and monoterpenes decreased with the increase in alcohol concentration, on the contrary the rates of phthalates increased. The relative dissolution rates of most active components were relatively high in water and low concentration alcohol than other solvents.
Liquid chromatography-mass spectrometry is practical for comprehensive multi-component assessment on traditional Chinese medicine preparation processes and can provide reference for optimization of processing parameters.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 04/2012; 37(7):916-9.
-
[show abstract]
[hide abstract]
ABSTRACT: To study the major metabolites of ferulic acid and gallic acid compatible with Danggui Chishaoyao in rat plasma and urine.
The metabolites of ferulic acid and gallic acid in rat plasma and urine were analyzed after oral administration of compatible Danggui Chishaoyao using UPLC-Q-TOF-MS.
On the basis of the mass information, it was inferred that in vivo metabolites of ferulic acid were be in the form of methylation products, sulfate conjugation products and glucuronidation conjugation products and so on; meanwhile, gallic acid was mainly transformed into eduction products and methylation products.
There are kinds of phase I and phase II metabolites of ferulic acid and gallic acid in rat plasma and urine, which provide a basis for its efficacious materials and action mechanism.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 02/2012; 37(3):366-72.
-
Yuhui Zhou,
Fei Xiong,
Zhenzhou Huang,
Luyu Zheng,
Miao Jiang,
Ming Jiang, Yuping Tang,
Jian Ma,
Zhen Zhan,
Jinao Duan,
Xu Zhang
01/2012; , ISBN: 978-953-307-903-5
