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ABSTRACT: Endoglin is a proliferation-associated antigen on endothelial cells and essential for angiogenesis. Soluble endoglin (s‑endoglin), formed by proteolytic cleavage of ectodomain of membrane receptor could be an indicator of tumor‑activated endothelium. The aim of present study was to analyze changes of s‑endoglin level in plasma of lung cancer patients following surgical resection and to estimate the correlation of s‑endoglin with other soluble receptors, sTie2 and sVEGF R1.
The study group consisted of 37 patients with stage I of non-small cell lung cancer. Plasma concentrations of s‑endoglin, sTie2 and sVEGF R1 were evaluated by ELISA, three times: before surgical resection and on postoperative day 7 and 30.
The median of s‑endoglin concentration decreased significantly on postoperative day 7 when compared with preoperative level and next increased on 30(th) day and it was comparable with that before surgery. s-Endoglin correlated with another soluble receptors, with sTie2 both before surgery (r=0.44) and on postoperative day 7 (r=0.52) and on 30(th) day (r=0.58), with sVEGF R1 - only on postoperative day 7 (r=0.75).
The increased level of serum endoglin in lung cancer patients compared to controls and its changes after surgical treatment suggest potential application of soluble form of endoglin as potential tumor marker.
Experimental oncology 03/2012; 34(1):53-6.
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ABSTRACT: Angiogenesis, formation of a new blood vessel from the existing vascular network, is essential for tumor growth, progression and metastasis. Vascular endothelial growth factor (VEGF) has been identified to be one of the most important factors of angiogenesis. VEGF-C, a novel member of the family, is a relatively specific lymphangiogenic growth factor. It is tempting to suggest that cervical cancer is one of the most common malignancies in a woman's life. Its prognostic factors are tumor stage, lymph node status, histologic type, level of hemoglobin. However, little is known about prognostic or/and predictive significance of angiogenesis in cervical cancer.
This prospective study is an attempt to evaluate serum VEGF-A, VEGF-C, microvessel density (MVD), and lymphatic vessel density (LMVD) in cervical cancer and the correlations with clinicopathologic features.
Blood samples were collected from 58 patients affected by FIGO I-IV stage cervical cancer, who were admitted to the Department of Oncology and Brachytherapy Collegium Medicum in Bydgoszcz of Nicolaus Copernicus University. Serum VEGF-A/VEGF-C concentrate was determined by means of a quantitative sandwich enzyme immunoassay (ELISA). All tumor samples were taken from cross section during the first brachytherapy. Then they were examined by immunohistochemical studies with podoplanin antibody and anti-CD31 antibody. The present analysis was used to evaluate MVD and LMVD.
The median serum VEGF-A was 734.76 pg/ml (range from 86.39 pg/ml - 2200.00 pg/ml), and VEGF-A was only correlated with after treatment hemoglobin concentration (p = 0.046, R = -0.3450). The median serum VEGF-C was 145.72 pg/ml (range 131.08 - 233.60 pg/ml). Serum VEGF-C levels measured in patients were associated with primary tumor size. We observed significantly higher serum VEGF-C in localized disease (FIGO I, II) in comparison to advanced tumors (232.44 pg/ml vs 152.45 pg/ml; p = 0.034). The median LMVD was 6.25 (range 3.5-10.0) and median blood vessel density was 12.5 (range 9.5-23.0). We found significantly higher lymphatic vessel density in patients with Gl/G2 grade of differentiation than in those with G3 (9.93 vs 6.25; p = 0.0398). We observed a statistically significant correlation between MVD and LMVD; (p = 0.032).
In conclusion, our study suggests that serum VEGF-A, VEGF-C, LMVD and MVD play an important role in tumor growth and progression in cervical cancer. Nonetheless, further studies are essential to explore the underlying mechanism.
European journal of gynaecological oncology 01/2012; 33(4):399-405. · 0.47 Impact Factor
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ABSTRACT: Angiogenesis is important in the promotion and progression of malignancy. The formation of new blood vessels is coordinated by many factors, angiopoietins among others. Overexpression of angiopoietins has been observed in various tumors. The aim of the study was to evaluate plasma concentration of Ang-1, Ang-2 and Tie-2 in cervical cancer.
The study group consisted of 34 patients with cervical cancer and the control group of 20 healthy volunteers. Plasma concentrations of Ang-1, Ang-2 and Tie-2 were evaluated by ELISA.
Plasma concentrations of Ang-1, Ang-2, Tie-2 and Ang-1/Ang-2 ratios were significantly higher in cervical cancer patients than in controls. Although there was no correlation between concentration of Ang-1, Ang-2, Tie-2 and clinical stage (FIGO), the Ang-1/Ang-2 ratio was higher in Stage I than in II-III. Ang-1 correlated positively with Ang-2 and Tie-2 in a subgroup with Stage II-III and Ang-2 with Tie-2 in a subgroup with Stage I.
Plasma concentrations of Ang- 1, Ang-2 and Tie-2 may be useful additional tumor markers in cervical cancer.
European journal of gynaecological oncology 01/2009; 30(6):646-9. · 0.47 Impact Factor
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ABSTRACT: The aim of the study was to determine the activity of cathepsin D (CTSD) and alpha-1-antitrypsin (AAT) in the blood serum of women with cervical carcinoma treated with different modes of therapy.
The study was conducted on 68 women suffering from carcinoma of the uterine cervix, that were irradiated intracavitarily by a Selectron LDR brachytherapy unit. Additionally, all patients were treated with different therapy methods according to clinical stage.
In women with cervical cancer, CTSD activity was higher while AAT activity was lower both before and after brachytherapy sessions as compared to controls. Six months after the end of therapy, the activity of CTSD and AAT reverted back to the values characteristic for healthy women.
The estimation of cathepsin D and alpha-1-antitrypsin activity during the course of cervical cancer management may be useful in early detection of potential recurrence and/or widespread metastasis formation.
European journal of gynaecological oncology 02/2008; 29(6):617-9. · 0.47 Impact Factor
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ABSTRACT: Selenium, as a component of few antioxidant enzymes, participates indirectly in elimination of reactive oxygen species and in antioxidative defense of the organism. There is a correlation between the concentration of selenium, activity of glutathione peroxidases (GSH-Px), and other parameters of antioxidative defense in blood components. The above mentioned factors were suggested to play an important role in etiopathogenesis of neoplastic diseases. Therefore, the aim of our present study was to compare the selenium status and GSH-Px activity in the plasma of 22 healthy women, 50 individuals suffering from cancer of uterine cervix, uterine corpus or ovary, and 49 women diagnosed with benign neoplasia of the uterine corpus or ovary. In addition, the selenium concentration was measured in postoperative cancer tissues, benign tumors, and histopatologically healthy surgical margins of the aforementioned patients. An average selenium concentration and GSH-Px activity in blood plasma of cancer patients and benign neoplasia patients was significantly lower than in the plasma of healthy women. It suggests that lower overall selenium status and lower selenium-dependent antioxidative capacity of the organism might partly contribute to development of neoplastic diseases of reproductive system. Postoperative tissues of patients revealed significantly higher selenium concentrations in cancer tissues of uterine cervix and corpus, and benign tumors of uterine corpus, as compared to corresponding healthy tissue margins. Higher accumulation of selenium in these neoplastic tissues might reflect a compensatory up-regulation of antioxidant defense systems in tumors that often undergo a persistent oxidative stress.
Neoplasma 02/2007; 54(5):374-8. · 1.44 Impact Factor
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ABSTRACT: The aim of the study was to determine the effect of different types of management on the activity of catalase (CAT) and glutathione peroxidase (GPx) in women with cervical carcinoma.
The patients were divided into three groups according to the mode of treatment. Patients from the first group were treated brachytherapy prior to surgery. The second group received teletherapy before brachytherapy and additionally chemotherapy. The third group was treated with teletherapy after brachytherapy sessions.
CAT activity was higher while GPx activity was lower before and during therapy in all groups as compared to controls. Six months after the end of therapy, the activity of studied enzymes reached the values characteristic of healthy women. No significant differences in enzyme activity among the three groups were revealed.
Normalization of CAT and GPx activity may prove the efficacy of applied therapy in cervical cancer patients, however enzyme activity recovery was not dependent on treatment mode.
European journal of gynaecological oncology 02/2007; 28(6):461-3. · 0.47 Impact Factor
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ABSTRACT: Cathepsins play an important role in the pathogenesis of the great number of cancer diseases i.e. in breast, head and neck, liver, pancreas, colon and skin cancer. Estimation of cathepsins activity referring to different kinds of cancer diseases has a significance both in diagnostics and in monitoring of their running. An essential role in cancer disease development is also reported for cathepsins B and D inhibitors. Using drugs that are able to inhibit the harmful activity of cathepsins, would give a chance to supplement an anti-cancer therapy with the new agents. In future, it could increase the efficiency of cancer treatment in a significant way.
Polski merkuriusz lekarski: organ Polskiego Towarzystwa Lekarskiego 08/2001; 11(61):88-90.
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ABSTRACT: The lysosomal proteases play an important role in the cells nourishment, immunogenesis, development of the arteriosclerosis perturbations in blood vessel cells and in the pathogenesis of degeneration diseases, cancer diseases and in the great number of others. For the many cancer disease durations, an increased activity of the lysosomal enzymes both in blood serum and in tumor tissues was revealed. Over the past few years a particular attention have been paid to the fact that the high activity of some lysosomal enzymes i.e. cathepsin B and D is connected with the increased cancer invasiveness. The results of preliminary clinical research point to great diagnostic value of cathepsin B and D in breast cancer detection.
Polski merkuriusz lekarski: organ Polskiego Towarzystwa Lekarskiego 02/2001; 10(55):65-70.
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ABSTRACT: A retrospective analysis of 23 patients with tracheal malignancy treated with a radiation therapy alone is reported. All patients were irradiated at Bydgoszcz Cancer Center during the period 1990-1996. To overcome serious damage to normal tissues, a dose escalation combination of external beam irradiation and brachytherapy was used in most cases. Squamous cell carcinoma was the most common type and was seen in 13 cases. Adenoid cystic carcinoma occurred in seven, adenocarcinoma in two and carcinoid in one patient. Eight patients were treated with definitive and 15 with palliative intent. Local control was attained in 8 of 23 patients and was more frequent for patients from curative group treated with doses greater than 60 Gy. The mean survival for all patients was 9.5 months, and 26 and 7.2 months for definitive and palliative group, respectively. Survival was strongly correlated to histologic type and response to radiotherapy.
Lung Cancer 07/1998; 20(3):169-74. · 3.43 Impact Factor
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R Makarewicz
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ABSTRACT: This is a retrospective analysis of 25 patients with recurrent carcinoma of the vulva treated by radiation therapy at Bydgoszcz Regional Cancer Center between 1980-95 years. Tumour complete regression were observed in 16 cases. Nine patients were alive without evidence of the disease with a follow-up from 20 to 76 months; median 35.2 months. The results showed that important prognostic significance had: the pattern of recurrence, time interval to relapse and size of recurrent tumour. Also, it seems that results of treatment strongly depends on the total dose of radiation used in the treatment.
Ginekologia polska 06/1998; 69(5):335-9. · 0.41 Impact Factor
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ABSTRACT: This report consists of a retrospective analysis of 33 patients with carcinoma of the vulva treated with radiation therapy alone at Bydgoszcz Regional Cancer Center between 1986-1996. Patients were divided into two subgroups, group I (N = 25) treated with curative intent and group II (N = 8) treated with palliative intent. Five year actuarial overall and disease free survival for patients treated in definitively manner was 36.0% and 28.0% respectively. The overall local control rate was 48.5% or 16/33 patients. There was a suggestion that better local control was obtained in patients who received gross tumour radiation dose with interstitial brachytherapy and external beam irradiation.
Ginekologia polska 06/1998; 69(5):340-3. · 0.41 Impact Factor
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ABSTRACT: The study reviews experience with the treatment of advanced renal cell cancer at Bydgoszcz Regional Cancer Center within a 10-year period from 1985 to 1996. The aim of this paper was to evaluate the value of postoperative radiotherapy. The medical records of 186 patients with locally advanced renal cell cancer were reviewed retrospectively. Postoperative radiation therapy with a median dose of 50.0 Gy/t was given in 114 patients. The overall and disease-free survival, the pattern of recurrences, time interval to recurrence were assessed. For all patients, the 5-year overall and disease-free survival rates were 36.2% and 30.5%, respectively. Non significant difference was observed in terms of 5-year overall and disease-free survival between the group of patients with postoperative radiotherapy and without, 37.9%/29.5% vs. 35.5%/31.3%, respectively. A total of 29 patients (15.6%) developed local recurrences. Local failure by stage was as follows: T3N0 without postoperative radiation therapy--15.8%, with irradiation--8.8%; T3N(+) without radiation therapy--33.3%, with irradiation--33.3%; T4N0 without radiation therapy--33.3%, with irradiation--33.3%, T4N(+) without radiation therapy--33.3%, with irradiation--25.0%. 73 patients (39.3%) had distant metastases as a first symptom of renal cell cancer relapse. The median time to relapse for local recurrence or distant metastases were approximately two times longer in patients with adjuvant radiotherapy compared to those without, 27.0/21.0 months vs. 16.0/12.5 months, respectively. In our opinion postoperative radiotherapy reduces the probability of local recurrences in selected patients, mainly with pathologic stage T3N0, but its impact on survival is minimal.
Neoplasma 02/1998; 45(6):380-3. · 1.44 Impact Factor
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ABSTRACT: Results of treatment of 10 patients with local-regionally advanced vulvar cancer are described. Loco-regional control of tumor was achieved in 6 patients. These patients remain disease-free with follow-up from 18 till 36 months. The procedure was well tolerated with low serious toxicity rate.
Ginekologia polska 10/1997; 68(9):427-31. · 0.41 Impact Factor
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ABSTRACT: In the period from 1980 to 1990 491 women operated for the cancer of the body of the uterus (stage I, II, III) underwent radiotherapy in the Department of Oncology and Radiotherapy and in Regional Oncological Centre in Bydgoszcz. The patients were at the age from 34 to 78. In the paper the patients' clinical condition was described and assessed according to FIGO/1972 and FIGO/1988 classifications the stages of progression of neoplasms were compared. The distant results of treatment depending on neoplasm's stage, histological type and the method of postoperative radiotherapy were presented.
Wiadomości lekarskie (Warsaw, Poland: 1960) 01/1997; 50(7-9):170-4.
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ABSTRACT: The prevalence of malignant neoplasms of the body of the uterus in women from Bydgoszcz province in the period 1980-1993, considering particularly the years 1990-1993 was presented. The performed epidemiologic investigations show the increase of incidence of this group of neoplastic diseases and the decrease of mortality.
Wiadomości lekarskie (Warsaw, Poland: 1960) 01/1997; 50(7-9):193-7.
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ABSTRACT: Remote afterloading high-dose-rate brachytherapy was used for the treatment in 38 patients with non-small cell lung cancer local failure after previous teleradiotherapy. The high dose unit we used is called microSelectron HDR. A dose of 18 Gy or 22.5 Gy was delivered in three fractions repeated weekly. Before treatment, symptomatic occlusions were seen in all patients. After treatment palliative improvement was seen in 30 patients (78.9%). The average follow up time was 7.2 months and range from 3 to 17. Median response time was 6.8 months. 12 patients died meanwhile. The procedure has been well tolerated with low toxicity. We conclude that palliative endobronchial high dose rate brachytherapy is a useful palliative modality in patients with recurrent endobronchial symptomatic carcinoma.
Pneumonologia i alergologia polska: organ Polskiego Towarzystwa Ftyzjopneumonologicznego, Polskiego Towarzystwa Alergologicznego, i Instytutu Gruzlicy i Chorob Pluc 02/1996; 64(7-8):444-9.
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ABSTRACT: The value of cathepsin B activity determination for evaluation of the extent of disease was investigated in 98 patients with the cervix uteri carcinoma and 25 women with cervix uteri dysplasia. The measurements were performed before treatment. Cathepsin B activity was estimated in serum using Z-Phe-Arg-NMec, and in tumor tissue using Z-Arg-Arg-pNA . HCl as substrates. The mean activity of the enzyme increased both in serum and tumor tissue with progression of neoplastic disease and was dependent on the clinical stage of cervical carcinoma. It should be stressed, however, that among the patients with the clinically observed early stage of the disease, higher cathepsin B activity was observed in those in whom metastases to pelvic lymph nodes were detected than in those in whom the disease was limited to cervix uteri.
Acta biochimica Polonica 02/1996; 43(3):521-4. · 1.49 Impact Factor
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ABSTRACT: The value of cathepsin B activity in determining the extent of disease was investigated in 106 patients with carcinoma of the cervix uteri and in 20 women with dysplasia of the cervix uteri. The measurements of serum and tumor cathepsin B activity were performed before treatment. The levels of cathepsin B were estimated by direct fluorimetric assay based on the use of 2-Phe-Arg-NMec as a substrate. The results show that the mean serum and tumor activity of this enzyme increase with progression of neoplastic disease and was closely dependent on clinical stage of cervical carcinoma. Also, we found higher serum and tumor levels of cathepsin B activities in patients with clinical early disease (FIGO Stage Ib and IIa) in whom metastases to pelvic lymph nodes were detected than in patients with disease limited to the cervix uteri (p < 0.001, t-test). Enhanced activity of cathepsin B was observed in 64% of patients with cervical carcinoma.
Neoplasma 02/1995; 42(1):21-4. · 1.44 Impact Factor
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ABSTRACT: Three patients with primary non-Hodgkin's lymphoma of the uterine cervix are described. The results of their treatment and a review of the international literature would support the statement that more patients with localized primary non-Hodgkin's lymphoma of the uterine cervix can be cured with radiotherapy, or with a combination of chemotherapy and irradiation.
Clinical Oncology 02/1995; 7(3):198-9. · 2.07 Impact Factor
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ABSTRACT: The activity of cathepsins B and D were determined in the serum in 98 patients with carcinoma of the cervix uteri and in 25 women with dysplasia of the cervix uteri. The clinical value of the examined enzymes was performed. The results show that the mean serum activity of cathepsins increase with progression of neoplastic disease. The elevation of at least one of two studied lysosomal hydrolases were estimated in 68 patients (69.5%) with carcinoma of the cervix uteri.
Ginekologia polska 11/1994; 65(10):578-81. · 0.41 Impact Factor
