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ABSTRACT: Objectives Three-dimensional (3D) umbilical cord blood volume flow measurement with the intention of providing a straightforward, consistent, and accurate method that overcomes the limitations associated with traditional pulsed wave Doppler flow measurement and provides a means by which to recognize and manage at-risk pregnancies. Methods The first study involved 3D sonographic volume flow measurements in 7 healthy ewes whose pregnancies ranged from 18 to 19 weeks' gestation (7 singletons). Sonographic umbilical arterial and venous flow measurements from each fetus were compared to the corresponding average measured arterial/venous flow to assess the feasibility of measurement in a static vessel. A second complementary study involved 3D sonographic volume flow measurements in 7 healthy women whose pregnancies ranged from 17.9 to 36.3 weeks' gestation (6 singletons and 1 twin). Umbilical venous flow measurements were compared to similar flow measurements reported in the literature. Pregnancy outcomes were abstracted from the medical records of the recruited patients. Results In the fetal sheep model, arterial/venous flow comparisons yielded errors of 10% or less for 8 of the 9 measurements. In the clinical study, venous flow measurements showed agreement with the literature over a range of gestational ages. Two of the 7 patients in the clinical study had lower flow than anticipated for gestational age; one had a subsequent diagnosis of intrauterine growth restriction, and the other had preeclampsia. Conclusions Accurate measurement of umbilical blood volume flow can be performed with relative ease in both the sheep model and in humans using the proposed 3D sonographic flow measurement technique. Results encourage further development of the method as a means for diagnosis and identification of at-risk pregnancies.
Journal of ultrasound in medicine: official journal of the American Institute of Ultrasound in Medicine 12/2012; 31(12):1927-34. · 1.25 Impact Factor
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ABSTRACT: Acoustic droplet vaporization (ADV) shows promise for spatial control and acceleration of thermal lesion production. The investigators hypothesized that microbubbles generated by ADV could enhance high-intensity focused ultrasound (HIFU) thermal ablation by controlling and increasing local energy absorption.
Thermal lesions were produced in tissue-mimicking phantoms using focused ultrasound (1.44 MHz) with a focal intensity of 4000 W · cm(-2) in degassed water at 37°C. The average lesion volume was measured by visible change in optical opacity and by T2-weighted magnetic resonance imaging. In addition, in vivo HIFU lesions were generated in a canine liver before and after an intravenous injection of droplets with a similar acoustic setup.
Thermal lesions were sevenfold larger in phantoms containing droplets (3 × 10(5) droplets/mL) compared to phantoms without droplets. The mean lesion volume with a 2-second HIFU exposure in droplet-containing phantoms was comparable to that made by a 5-second exposure in phantoms without droplets. In the in vivo study, the average lesion volumes without and with droplets were 0.017 ± 0.006 cm(3) (n = 4; 5-second exposure) and 0.265 ± 0.005 cm(3) (n = 3; 5-second exposure), respectively, a factor of 15 difference. The shape of ADV bubbles imaged with B-mode ultrasound was very similar to the actual lesion shape as measured optically and by magnetic resonance imaging.
ADV bubbles may facilitate clinical HIFU ablation by reducing treatment time or requisite in situ total acoustic power and provide ultrasonic imaging feedback of the thermal therapy.
Academic radiology 06/2011; 18(9):1123-32. · 2.09 Impact Factor
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ABSTRACT: Ultrasound can be used to release a therapeutic payload encapsulated within a perfluorocarbon (PFC) emulsion via acoustic droplet vaporization (ADV), a process whereby the PFC phase is vaporized and the agent is released. ADV-generated microbubbles have been previously used to selectively occlude blood vessels in vivo. The coupling of ADV-generated drug delivery and occlusion has therapeutically synergistic potentials.
Micron-sized, water-in-PFC-in-water (W(1)/PFC/W(2)) emulsions were prepared in a two-step process using perfluoropentane (PFP) or perfluorohexane (PFH) as the PFC phase. Fluorescein or thrombin was contained in the W(1) phase.
Double emulsions containing fluorescein in the W(1) phase displayed a 5.7±1.4-fold and 8.2±1.3-fold increase in fluorescein mass flux, as measured using a Franz diffusion cell, after ADV for the PFP and PFH emulsions, respectively. Thrombin was stably retained in four out of five double emulsions. For three out of five formulations tested, the clotting time of whole blood decreased, in a statistically significant manner (p < 0.01), when incubated with thrombin-loaded emulsions exposed to ultrasound compared to emulsions not exposed to ultrasound.
ADV can be used to spatially and temporally control the delivery of water-soluble compounds formulated in PFC double emulsions. Thrombin release could extend the duration of ADV-generated, microbubble occlusions.
Pharmaceutical Research 12/2010; 27(12):2753-65. · 4.09 Impact Factor
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ABSTRACT: Clinical volumetric blood flow estimation relies on several assumptions. Among them are cylindrical vessel geometry, symmetric flow profile, and Doppler angle. None of them are known well enough to obtain clinically relevant estimates. 3-D color flow acquisition circumvents these assumptions, posing a viable tool for in vivo blood volume flow analysis. A 4-D cardiac scanner operating a 2-D array for real-time 3-D color flow imaging [GE Healthcare, Milwaukee, WI] was used. The array was positioned to fully intersect a 2-cm-diameter flow tube with the constant-depth plane (CPlane). Blood mimicking fluid was circulated at up to 6 l∕min using a cardiac bypass pump (60 and 80 beats∕min). A trigger source synchronized the pump and scanner. Data volumes were acquired equally spaced throughout the cardiac cycle. Temporally resolved volume flow was derived from CPlane data integration using Doppler power partial volume correction. Results show less than 7% mean flow error for temporally resolved volume flow (100 points per cardiac cycle and 50 averages). Single points in the cardiac cycle (10 max total), including systolic and diastolic flow, can be acquired within 50 heartbeats. Average, nontime-resolved flow can be estimated within 5 s.
The Journal of the Acoustical Society of America 10/2010; 128(4):2303. · 1.55 Impact Factor
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ABSTRACT: Ultrasound-mediated delivery systems have mainly focused on microbubble contrast agents as carriers of drugs or genetic material. This study uses micron-sized, perfluoropentane (PFP) emulsions as carriers of chlorambucil (CHL), a lipophilic chemotherapeutic. The release of CHL is achieved via acoustic droplet vaporization (ADV), whereby the superheated emulsion is converted into gas bubbles using ultrasound. Emulsions were made using an albumin shell and soybean oil as the CHL carrier. The ratio of the PFP to soybean oil phases in the droplets and the fraction of droplets that vaporize per ultrasound exposure were shown to correlate with droplet diameter. A 60-min incubation with the CHL-loaded emulsion caused a 46.7% cellular growth inhibition, whereas incubation with the CHL-loaded emulsion that was exposed to ultrasound at 6.3 MHz caused an 84.3% growth inhibition. This difference was statistically significant (p < 0.01), signifying that ADV can be used as a method to substantially enhance drug delivery.
Ultrasound in medicine & biology 08/2010; 36(8):1364-75. · 2.02 Impact Factor
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ABSTRACT: Micron- and nano-sized colloids are being studied in diagnostic and therapeutic applications of ultrasound (US). Unlike clinically utilized microbubbles, emulsions possess unique physiochemical properties that could translate into distinct, clinical benefits beyond conventional contrast agents. Droplets, composed of a superheated liquid, can be phase transitioned into bubbles using US, a process known as acoustic droplet vaporization (ADV). Droplets, transpulmonary in size, transition into bubbles upon ADV and can reach diameters that occlude capillaries and arrest blood flow in the vascular bed. Examples of ADV in diagnostic and therapeutic applications will be presented. First, ADV has been used in phase aberration correction in transcranial US imaging. Second, ADV-generated microbubbles can reduce and occlude renal perfusion in vivo. Third, the effects of thermal therapy using high-intensity focused ultrasound (HIFU) has been enhanced and controlled more effectively using ADV. Fourth, ADV has been utilized as a release mechanism for therapeutic agents that are incorporated into the emulsion. In all applications, the physiochemical properties of the droplets coupled with the spatial and temporal control afforded by ADV-generated microbubbles are crucial to the success of each ADV development. [This work was supported in part by NIH Grant 5R01EB000281.].
The Journal of the Acoustical Society of America 03/2010; 127(3):1975. · 1.55 Impact Factor
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ABSTRACT: To verify a previously reported three-dimensional (3D) ultrasound method for the measurement of time-average volumetric blood flow, experiments were performed under pulsatile flow conditions, including in vivo investigations, and results were compared with accepted, but invasive, "gold standard" techniques. Results showed that volume averaging results in the correct time-average volume flow without the need for cardiac gating. Unlike other currently employed methods, this method is independent of Doppler angle, flow profile and vessel geometry. A GE Logiq 9 ultrasound system (GE Medical Systems, Milwaukee, WI, USA) and a four-dimensional (4D) 10L and 4D 16L probe were used to acquire 3D Doppler measurements in the femoral and carotid arteries of four canines. Two invasive blood flow meters were used (electromagnetic for one canine and ultrasonic for three canines) as the gold standard techniques. Transcutaneous color flow measurements were taken to obtain 3D volume data sets encompassing the vessel. Constant depth planes were used to integrate color flow pixels encompassing the entire vessel cross-section. Power Doppler data were used to correct for partial volume effects. An artificial stenosis was induced to vary the ambient volume flow. Unrestricted, bidirectional flow was measured as high as 400 mL min(-1). Several flow restrictions were imposed that decreased the measured volumetric flow rate to as low as 30 mL min(-1). All flow rate estimates (n=38) were plotted against results obtained via the gold standards. A general line fit resulted in y = 0.926 x - 0.87 (r(2) = 0.95), which corresponds to a 0.6% flow offset relative to the average flow rate of 142 mL min(-1), as well as a 7.4% error in the linearity of our estimate. A secondary curve fit was performed that required the slope to be 1 and the intercept to be 0, which yielded an r(2)-value of 0.93. The percent-error distribution was computed and fitted to a Gaussian function, which yielded mu=-7.04% and sigma=9.52%. Theoretical studies were conducted to estimate the expected error in our volume flow measurements as a function of number of samples (N) used for averaging pulsatile waveforms. Experiments showed the same 1/N dependence as theory. Direct comparisons of volume flow rate estimates using volumetric color Doppler and independent standards showed that our method has good accuracy under in vivo pulsatile blood flow conditions.
Ultrasound in medicine & biology 10/2009; 35(11):1880-91. · 2.02 Impact Factor
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ABSTRACT: A theoretical shot noise model to describe the output of a time-reversal experiment in a multiple-scattering medium is developed. This (non-wave equation based) model describes the following process. An arbitrary waveform is transmitted through a high-order multiple-scattering environment and recorded. The recorded signal is arbitrarily windowed and then time-reversed. The processed signal is retransmitted into the environment and the resulting signal recorded. The temporal and spatial signal and noise of this process is predicted statistically. It is found that the time when the noise is largest depends on the arbitrary windowing and this noise peak can occur at times outside the main lobe. To determine further trends, a common set of parameters is applied to the general result. It is seen that as the duration of the input function increases, the signal-to-noise ratio (SNR) decreases (independent of signal bandwidth). It is also seen that longer persisting impulse responses result in increased main lobe amplitudes and SNR. Assumptions underpinning the generalized shot noise model are compared to an experimental realization of a multiple-scattering medium (a time-reversal chaotic cavity). Results from the model are compared to random number numerical simulation.
The Journal of the Acoustical Society of America 06/2009; 125(5):3129-40. · 1.55 Impact Factor
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ABSTRACT: The vaporization of a superheated droplet emulsion into gas bubbles using ultrasound--termed acoustic droplet vaporization (ADV)--has potential therapeutic applications in embolotherapy and drug delivery. The optimization of ADV for therapeutic applications can be enhanced by understanding the physical mechanisms underlying ADV, which are currently not clearly elucidated. Acoustic cavitation is one possible mechanism. This paper investigates the relationship between ADV and inertial cavitation (IC) thresholds (measured as peak rarefactional pressures) by studying parameters that are known to influence the IC threshold. These parameters include bulk fluid properties such as gas saturation, temperature, viscosity, and surface tension; droplet parameters such as degree of superheat, surfactant type, and size; and acoustic properties such as pulse repetition frequency and pulse width. In all cases the ADV threshold occurred at a lower rarefactional pressure than the IC threshold, indicating that the phase transition occurs before IC events. The viscosity and temperature of the bulk fluid are shown to influence both thresholds directly and inversely, respectively. An inverse trend is observed between threshold and diameter for droplets in the 1 to 2.5 microm range. Based on a choice of experimental parameters, it is possible to achieve ADV with or without IC.
IEEE transactions on ultrasonics, ferroelectrics, and frequency control 06/2009; 56(5):1006-17. · 1.80 Impact Factor
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ABSTRACT: Time-reversal acoustics has been shown to be effective at focusing sound through high-order multiple-scattering media. In addition to signals being refocused, large amplitudes via pulse compression have also been observed. Previously, a statistical model was developed to simulate a time-reversal experiment where an impulse is sent into the scattering medium and the entire response is time-reversed [A. Derode et al., J. App. Phys. 85, 6343-6352 (1999)]. The high-order multiple-scattering process is modeled as a shot noise process. Again postulating a shot noise process the model has been extended to allow for arbitrary input functions and arbitrary windowing before time-reversal. The model has been shown to accurately predict the variance and expectation value of ensembles of simulated data. The analytical model is also tied directly to the governing physical processes, which is useful in attempting to optimize refocused signals. This extension is motivated by a desire to achieve high-amplitude long-pulse focusing, as would be useful in thermal therapies and radiation force experiments. However, the model can find application beyond this specific end-point. The model, it's physical implications, and several time-reversal simulations will be presented.
The Journal of the Acoustical Society of America 06/2008; 123(5):3341. · 1.55 Impact Factor
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ABSTRACT: We report on the first experiments demonstrating the transcranial acoustic formation of stable gas bubbles that can be used for transcranial ultrasound aberration correction. It is demonstrated that the gas bubbles can be formed transcranially by phase-transitioning single, superheated, micron-size, liquid dodecafluoropentane droplets with ultrasound, a process known as acoustic droplet vaporization (ADV). ADV was performed at 550 kHz, where the skull is less attenuating and aberrating, allowing for higher-amplitudes to be reached at the focus. Additionally, it is demonstrated that time-reversal focusing at 1 MHz can be used to correct for transcranial aberrations with a single gas bubble acting as a point beacon. Aberration correction was performed using a synthetic aperture approach and verified by the realignment of the scattered waveforms. Under the conditions described below, time-reversal aberration correction using gas bubbles resulted in a gain of 1.9 +/- 0.3 in an introduced focusing factor. This is a small fraction of the gain anticipated from complete transmit-receive of a fully-populated two-dimensional array with sub-wavelength elements. (E-mail: khaworth@umich.edu).
Ultrasound in Medicine & Biology 04/2008; 34(3):435-45. · 2.29 Impact Factor
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ABSTRACT: It was hypothesized that the use of internal finger structure as imaged using commercially available ultrasound (US) scanners could act as a supplement to standard methods of biometric identification, as well as a means of assessing physiological and cardiovascular status. Anatomical structures in the finger including bone contour, tendon and features along the interphalangeal joint were investigated as potential biometric identifiers. Thirty-six pairs of three-dimensional (3D) gray-scale images of second to fourth finger (index, middle and ring) data taken from 20 individuals were spatially registered using MIAMI-Fuse software developed at our institution and also visually matched by four readers. The image-based registration met the criteria for matching successfully in 14 out of 15 image pairs on the same individual and did not meet criteria for matching in any of the 12 image pairs from different subjects, providing a sensitivity and specificity of 0.93 and 1.00, respectively. Visual matching of all image pairs by four readers yielded 96% successful match. Power Doppler imaging was performed to calculate the change in color pixel density due to physical exercise as a surrogate of stress level and to provide basic physiological information. (E-mail: gnarayan@umich.edu).
Ultrasound in Medicine & Biology 04/2008; 34(3):392-9. · 2.29 Impact Factor
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ABSTRACT: The use of superheated liquid perfluorocarbon droplets encased in albumin shells has been proposed as a minimally invasive alternative to current treatment of cancer by means of occlusion therapy. In response to an applied acoustic field, these droplets, which are small enough to pass through capillaries, vaporize into large gas bubbles that subsequently lodge in the vasculature. This technique, known as acoustic droplet vaporization (ADV) has been shown to successfully reduce blood flow in vivo, but for in situ conditions where attenuation is present, lower acoustic frequency and ADV threshold may be desirable. Thus, two methods to lower the ADV threshold at a lower 1.44 MHz were explored. The first part of this study investigated the role of pulse duration on ADV. The second part investigated the role of inertial cavitation (IC) external to a droplet by lowering the IC threshold in the host liquid with the presence of Definity contrast agent (CA). The threshold was found to be 5.5-5.9 MPa for short microsecond pulses and decreased for millisecond pulses (3.8-4.6 MPa). When CAs were present and long millisecond pulses were used, the ADV threshold decreased to values as low as 0.41 MPa.
IEEE Transactions on Ultrasonics Ferroelectrics and Frequency Control 06/2007; 54(5):933-46. · 1.69 Impact Factor
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ABSTRACT: This paper describes a simple microfluidic sorting system that can perform size profiling and continuous mass-dependent separation of particles through combined use of gravity (1 g) and hydrodynamic flows capable of rapidly amplifying sedimentation-based separation between particles. Operation of the device relies on two microfluidic transport processes: (i) initial hydrodynamic focusing of particles in a microchannel oriented parallel to gravity and (ii) subsequent sample separation where positional difference between particles with different mass generated by sedimentation is further amplified by hydrodynamic flows whose streamlines gradually widen out due to the geometry of a widening microchannel oriented perpendicular to gravity. The microfluidic sorting device was fabricated in poly(dimethylsiloxane), and hydrodynamic flows in microchannels were driven by gravity without using external pumps. We conducted theoretical and experimental studies on fluid dynamic characteristics of laminar flows in widening microchannels and hydrodynamic amplification of particle separation. Direct trajectory monitoring, collection, and post-analysis of separated particles were performed using polystyrene microbeads with different sizes to demonstrate rapid (<1 min) and high-purity (>99.9%) separation. Finally, we demonstrated biomedical applications of our system by isolating small-sized (diameter <6 microm) perfluorocarbon liquid droplets from polydisperse droplet emulsions, which is crucial in preparing contrast agents for safe, reliable ultrasound medical imaging, tracers for magnetic resonance imaging, or transpulmonary droplets used in ultrasound-based occlusion therapy for cancer treatment. Our method enables straightforward, rapid, real-time size monitoring and continuous separation of particles in simple stand-alone microfabricated devices without the need for bulky and complex external power sources. We believe that this system will provide a useful tool to separate colloids and particles for various analytical and preparative applications and may hold potential for separation of cells or development of diagnostic tools requiring point-of-care sample preparation or testing.
Analytical Chemistry 03/2007; 79(4):1369-76. · 5.86 Impact Factor
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ABSTRACT: The purpose of this study was to evaluate a 3-dimensional (3D) sonographic method for the measurement of volumetric flow under conditions of known flow rates and Doppler angles.
A GE/Kretz Voluson 730 system (GE Healthcare, Milwaukee, WI) and RAB2-5 probe were used to acquire 3D Doppler measurements in a custom flow phantom. Blood-mimicking fluid circulated by a computer-controlled pump provided a range of flow velocities (2-15 mL/s). A 6-axis positioning system maneuvered the ultrasound probe through a range of angles (40 degrees-70 degrees and 110 degrees -140 degrees) with respect to the tube (orthogonal to the tube being 90 degrees). Volume data sets were obtained spanning 29 degrees lateral and 20 degrees elevational angles encompassing the flow tube in a scanning time of less than 10 seconds. Power Doppler data were used to correct for partial volume effects.
Using a single angle (110 degrees) with respect to the flow tube, measured and actual volume flow rates were within the 95% confidence interval over the full range of flow rates. At flow rates of 5 and 10 mL/s, the measured volume flow rates were all within +/-15% of actual values for the range of angles tested and also stayed within the 95% confidence interval.
Direct comparisons of volume flow rates estimated with 3D sonography and known flow rates showed that the method has good accuracy. Subsequent comparisons under pulsatile and in vivo conditions will be needed to verify this performance for clinical applications.
Journal of ultrasound in medicine: official journal of the American Institute of Ultrasound in Medicine 11/2006; 25(10):1305-11. · 1.25 Impact Factor
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ABSTRACT: Vector Doppler methods are used to obtain angle independent in-plane velocity information. Velocity magnitude as well as direction are reconstructed from regular steered colour flow and from split-aperture Doppler acquisitions. Spatially resolved in-plane velocity was obtained through Doppler colour flow mode and subsequent data triangulation. A depth-invariant constant Doppler angle was achieved by using a depth expanding transmit-receive Doppler aperture. Velocities of up to 50 cm s(-1) and 360 degrees vector velocity directions were measured. This was achieved by creating a spinning solid disc phantom. Such a phantom was built to allow underwater mounting and spinning of a solid disc-shaped ultrasound phantom (maximum velocity of 50 cm s-1). Doppler triangulation was realised by steered Doppler and by a split-aperture approach. Results of both imaging methods are shown. Split-aperture results showed errors of less then 10% for velocity magnitude estimation and less then 2.5 degrees for directional information.
Ultrasound in Medicine & Biology 08/2006; 32(7):1037-46. · 2.29 Impact Factor
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ABSTRACT: Because microbubbles can enhance therapy, such as by cavitation or by thermal means, treatment could be confined with localization of microbubbles. This spatial control can be achieved by the vaporization of liquid-filled droplets present throughout the medium in a process known as acoustic droplet vaporization (ADV). Bubbles in the form of an orthogonal plane or "wall" can thus be created and can scatter ultrasound to enhance the proximal acoustic field while shielding distal tissues. To investigate the possible effects of a preexistent bubble wall, tissue-mimicking polyacrylamide gels embedded with perfluorocarbon droplets were insonified under various conditions. The preliminary results presented in this paper show that a bubble wall can successfully cause proximal ADV at approximately half the transmitted pressures that are required without the use of a bubble wall, while also serving as a viable shield against ADV and potential damage in distal areas. The results seen here in a gel medium are promising and suggest further development in vivo is needed.
Ultrasound in Medicine & Biology 02/2006; 32(1):95-106. · 2.29 Impact Factor
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ABSTRACT: Acoustic droplet vaporization (ADV) has been introduced with the potential application of tumor treatment via occlusion and subsequent necrosis. New Zealand White rabbits were anesthetized, and their left kidney was externalized. An imaging array and single-element transducer were positioned in a tank with direct access to the kidney's vasculature and renal artery. Filtered droplet emulsions (diameter <6 microm) were injected intra-arterially (IA) into the left heart during insonification of the renal artery, and the extent of blood flow reduction by ADV was compared to the untreated right kidney. Flow cytometry (using colored microspheres) of kidney tissue samples and reference blood from the femoral artery allowed the quantitative estimation of regional blood flow. A maximum regional blood flow reduction in the treated region of >90% and an average organ perfusion reduction of >70% was achieved using ADV. After treatment of the left kidney, the control kidney on the contralateral side showed a maximum decrease in regional blood flow of 18% relative to the pre-ADV baseline. Image-based hyper-echogenicity from ADV of IA injections was monitored for approximately 90 minutes, and cortex perfusion was reduced by >60% of its original value for more than 1 hour. This could be enough time for the onset of cell death and possible tumor treatment via ischemic necrosis. Moreover, currently used radiofrequency tissue ablation-based tumor treatment could benefit from ADV due to the decreased heat loss via vascular cooling.
IEEE Transactions on Ultrasonics Ferroelectrics and Frequency Control 07/2005; 52(7):1101-10. · 1.69 Impact Factor
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ABSTRACT: To evaluate the capability of intraoperative color-duplex sonography to detect eloquent flow-activated areas and their anatomic relationship in dogs.
After craniotomy, the sensory cortex of eight dogs was identified by recording the highest amplitude detected with a grid electrode evoked with somatosensory evoked potential stimulation of the nervus ischiadicus. A 7.5-MHz linear array transducer was placed on the dura, and eight images were taken in color-coded capture mode during baseline and somatosensory evoked potential stimulation of the ipsilateral (nonevoked) and contralateral (evoked) sensory cortex. The differences in flow velocity intensities were statistically compared (Wilcoxon test) in three arbitrary velocity ranges and across all colored pixels in a region of interest between baseline and stimulation in both hemispheres.
Comparing both hemispheres during stimulation, the evoked sensory cortex demonstrated an increase of 10% in the number of counted colored pixels during stimulation, whereas the number of counted colored pixels in the ipsilateral sensory cortex decreased by 2% (P < 0.05), indicating an overall increase in measured flow during stimulation. Comparing differences during nonstimulation and stimulation in single hemispheres, the lowest of the three velocity ranges (approximately 10-20 mm/s) demonstrated a statistically significant (P = 0.01) increase during stimulation, whereas no change was observed during stimulation in the ipsilateral hemisphere. This increase has been confirmed by regional cerebral blood flow measurement with colored microspheres.
This study indicates, for the first time, the capability of intraoperative ultrasound to detect functionally important areas during evoked stimulation.
Neurosurgery 03/2005; 56(2):355-63; discussion 355-63. · 2.79 Impact Factor
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ABSTRACT: This paper examines the vaporization of individual dodecafluoropentane droplets by the application of single ultrasonic tone bursts. High speed video microscopy was used to monitor droplets in a flow tube, while a focused, single element transducer operating at 3, 4, or 10 MHz was aimed at the intersection of the acoustical and optical beams. A highly dilute droplet emulsion was injected, and individual droplets were positioned in the two foci. Phase transitions of droplets were produced by rarefactional pressures as low as 4 MPa at 3 MHz using single, 3.25 micros tone bursts. During acoustic irradiation, droplets showed dipole-type oscillations along the acoustic axis (average amplitude 1.3 microm, independent of droplet diameter which ranged from 5 to 27 microm). The onset of vaporization was monitored as either spot-like, within the droplet, or homogeneous, throughout the droplet's imaged cross section. Spot-like centers of nucleation were observed solely along the axis lying parallel to the direction of oscillation and centered on the droplet. Smaller droplets required more acoustic intensity for vaporization than larger droplets, which is consistent with other experiments on emulsions.
The Journal of the Acoustical Society of America 08/2004; 116(1):272-81. · 1.55 Impact Factor
