[show abstract][hide abstract] ABSTRACT: In order to assess the influence of long-term irrigation of polluted river water on the ecological health of the rice fields in the low reach of Liaohe River Plain, a series of environmental samples and biological samples in rice fields, including sediments, paddy soils, mitten-handed crabs (Eriocheir sinensis), loaches (Misgurnus anguillicaudatu) and frog (Hoplobatrachus tigerinus), were collected from the area far away from industrial zones, and polychlorinated biphenyls (PCBs) and organochlorine pesticides (HCH, DDT, HCB and Mirex) were analyzed by high resolution gas chromatography coupled with high resolution mass spectrometry (HRGC/HRMS). The comparison of pollutant concentrations and profiles verified that the significant contribution of river water irrigation to PCBs contaminations in paddy soils and the mutual influence of organochlorine pesticide contamination between river sediments and paddy soils. Bioaccumulations of PCBs and organochlorine pesticides in mitten-handed crabs and loaches were site-specific, suggested that they were both the good indicators of ecosystem health. Mitten-handed crabs had the highest capacity to accumulate PCBs and organochlorine pesticides, and also they had the strong ability to degrade these pollutants in their bodies, especially for DDT and HCH. The biota-soil accumulation factors (BSAF) of ΣHCH, ΣDDT, Mirex, HCB and ΣPCB in mitten-handed crab were calculated to be 24.5, 15.3, 122.2, 28.1 and 54.6, respectively. In view of the strong bioaccumulation ability, the health risk should be concerned for the cultivation of mitten-handed crabs in paddy fields in the low reach of Liaohe River Plain.
Chinese Science Bulletin 08/2013; 58(15). · 1.32 Impact Factor
[show abstract][hide abstract] ABSTRACT: A new cleanup method was developed and validated for the determination of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/Fs) in sediment. The sample extract was first treated with sulfuric acid and then cleaned up by a large volume injection gas chromatography online coupled with liquid chromatography (LVI-GC-LC) system. PCDD/Fs in the extract were separated by a GC column (DB-5), selected cut, cool trapped and transferred to a LC column (alumina). The fraction of PCDD/Fs eluted from the alumina column was collected and concentrated for the instrumental analysis. Under the optimized conditions, LVI-GC-LC method achieved the recoveries of 57-102% for 2,3,7,8-substituted PCDD/Fs, which met the requirements of US Environmental Protection Agency (EPA) Method 1613 and were better than those obtained using the conventional multistep column cleanup method. Meanwhile, compared with the conventional method, the limit of detection (LOD) values of 2,3,7,8-substituted PCDD/Fs cleaned up by LVI-GC-LC method were decreased due to the high-efficiency removal of interferents. These results suggested that the LVI-GC-LC cleanup method was a promising alternative to the multistep cleanup procedure for the determination of dioxins in environmental samples.
[show abstract][hide abstract] ABSTRACT: Polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/Fs) are ubiquitous contaminants and can be considerably accumulated by natural plants. In order to elucidate the biochemical and physiological responses of plant to PCDD/Fs, tobacco Bright Yellow-2 (BY-2) cells were selected as model plant and treated with time- and concentration-dependent PCDD/Fs. The toxic effect and oxidative stress caused by PCDD/Fs were evident, which could be indicted by the reduction in fresh mass, the increase in malondialdehyde (MDA) content, and the damage of tobacco cell ultrastructure. PCDD/Fs tolerance was correlated with changes in antioxidant system and hormones of tobacco cells. Superoxide dismutase (SOD) and peroxidase (POD) exhibited peak enzyme activities at the PCDD/Fs concentration of 1000ng WHO(98)-TEQg(-1)fresh weight. Glutathione reductase (GR) enzyme activity increased monotonically at high level PCDD/Fs, but the activity of catalase (CAT) was only slightly affected at all treatment. Meanwhile, the exposure to PCDD/Fs resulted in the changes of hormones content. With the increase of exposure concentration of PCDD/Fs, the levels of indole-3-acetic acid (IAA) and abscisic acid (ABA) increased, whereas the concentration of jasmonates (JAs) decreased. The above results suggest that tobacco cells had the ability to cope with the oxidative stress induced by low concentration of PCDD/Fs through increasing the activities of antioxidant enzymes and alternating plant hormones levels. However, oxidative stress and toxicity would burst out when plant cells were exposed to the high levels of PCDD/Fs.
[show abstract][hide abstract] ABSTRACT: The influence of cadmium on growth, cadmium accumulation, composition, and content of glucosinolates was investigated in Arabidopsis thaliana after 4weeks of growth in hydroponics. Accumulation of 3,820 and 321μg Cd g−1 dry weight in the roots and leaves of A. thaliana, respectively, exposed to 50μM Cd. Cadmium treatment significantly decreased the total concentration of glucosinolates both
in the leaves and roots. Cd-induced alteration of total glucosinolate content in the roots was mainly due to the decrease
of indolyl-glucosinolates. In the Cd treatment leaves, significant decreases were, respectively, detected for glucoibervirin
and 4-methoxyglucobrassicin (P < 0.01), while other glucosinolate levels did not decrease significantly. In response to cadmium, the three indolyl-glucosinolates
all showed significant decreases in the roots. The distinctive influence of cadmium on glucosinolate profiles in Cd-sensitive
A. thaliana may be of great ecological importance, decreasing the resistance to phytophage attack. Taken together, our data is discussed
in relation to jasmonic acid and salicylic acid as possible molecules that modulate the alteration of glucosinolate profiles
in response to cadmium. The similar effects of Cd treatment on the levels of individual glucosinolates in leaves and roots
were observed at higher-concentration cadmium treatment (100μM Cd).
Water Air and Soil Pollution 04/2012; 200(1):109-117. · 1.75 Impact Factor
[show abstract][hide abstract] ABSTRACT: The performances of three adsorbents, i.e. silica gel, neutral and basic alumina, in the separation of short chain polychlorinated n-alkanes (sPCAs) from potential interfering substances such as polychlorinated biphenyls (PCBs) and organochlorine pesticides were evaluated. To increase the cleanup efficiency, a two-step cleanup method using silica gel column and subsequent basic alumina column was developed. All the PCB and organochlorine pesticides could be removed by this cleanup method. The very satisfying cleanup efficiency of sPCAs has been achieved and the recovery in the cleanup method reached 92.7%. The method detection limit (MDL) for sPCAs in sediments was determined to be 14 ng g(-1). Relative standard deviation (R.S.D.) of 5.3% was obtained for the mass fraction of sPCAs by analyzing four replicates of a spiked sediment sample. High resolution gas chromatography/electron capture negative ion-low resolution mass spectrometry (HRGC/ECNI-LRMS) was used for sPCAs quantification by monitoring [M-HCl](-) ions. When applied to the sediment samples from the mouth of the Daliao River, the optimized cleanup method in conjunction with HRGC/ECNI-LRMS allowed for highly selective identifications for sPCAs. The sPCAs levels in sediment samples are reported to range from 53.6 ng g(-1) to 289.3 ng g(-1). C(10)- and C(11)-PCAs are the dominant residue in most of investigated sediment samples.
[show abstract][hide abstract] ABSTRACT: A novel vapor dynamic headspace enrichment device was set up for nontarget screening of volatile organic compounds (VOCs) in drinking water. The main operating parameters of this device, such as length of distillation tube, volume of collected condensate, and choice of absorbent, were optimized. In this device, vapor was utilized as a purge gas and water was utilized as a absorbent. With the help of the device, one liter of water sample could be concentrated to 5 mL and the sensitivity of traditional purge and trap-gas chromatography-mass spectrometry (P&T-GC-MS) could be improved 1-2 orders of magnitude. Source and disinfected water samples from a water treatment plant were analyzed with this method. Compared with the traditional P&T-GC-MS analysis without pre-enrichment, the numbers of identified VOCs were improved from 0 to 16 for source water and 5 to 35 for disinfected water samples. It is also shown that there are many halide compounds in VOCs in disinfected water which do not exist in source water.
Se pu = Chinese journal of chromatography / Zhongguo hua xue hui 09/2011; 29(9):912-7.
[show abstract][hide abstract] ABSTRACT: In this work, an electrochemical DNA biosensor based on double-stranded DNA modified Au electrode (dsDNA/Au) was proposed for the rapid screening and detection of chlorinated benzenes pollutants, in which redox-active methylene blue (MB) was used to amplify the interaction between dsDNA and the target analyte. Using hexachlorobenzene (HCB) as a model analyte of chlorinated benzenes, the biosensor demonstrated a linear response with the logarithm of HCB concentrations from 100 pmol L(-1) to 100 nmol L(-1). The obtained detection limit was 30 pmol L(-1), which was remarkably superior to other biosensors. The interaction mechanism of the biosensor with HCB was proposed based on systematical characterization by cyclic voltammetry (CV), differential pulse voltammetry (DPV), UV-vis spectrometry and electrochemical quartz crystal microbalance (EQCM). Further studies revealed that the biosensor could screen chlorinated benzenes in the presence of 100 fold amount of other co-existing chemicals (ethyl acetate and sodium oxalate, etc.), and the response signal of the biosensors for different chlorinated benzenes was correlative to their respective toxicity. The proposed biosensor proved to be a promising "alarm" tool for rapid screening of chlorinated benzenes in real water samples.
[show abstract][hide abstract] ABSTRACT: The concentrations, compositional profiles, and potential ecological risk of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), polychlorinated biphenyls (PCBs) and polychlorinated naphthalenes (PCNs) in sediments of the Daliao River Estuary were investigated. Total concentrations of PCDD/Fs, PCBs, and PCNs were in the range of 11.3-133.2 ng/kg dry weight (dw), 1 971-37 632 ng/kg dw and 33.1-284.4ng/kg dw, respectively. The total TEQ values varied from 0.37 to 4.08ng/kg dw, with the dominant contributions by PCDD/Fs, then by PCBs and PCNs. The spatial distributions of PCDD/Fs, PCBs and PCNs in the river estuary were much related to hydrodynamic conditions. The risk of contamination to the deeper sea was increased in the flood seasons. Moreover, our data confirmed that both organic matter in sediments and molecular properties of dioxin-like compounds were the factors which strongly influenced the partition behavior of these dioxin-like compounds between sediments and water phase in the estuarine zone.
[show abstract][hide abstract] ABSTRACT: The concentrations, compositional profiles, possible sources of polybrominated diphenyl ethers (PBDEs) in sediments of the Daliao River Estuary as well as the factors influencing the distribution of PBDEs were investigated. The total concentrations of PBDEs ranged from 0.13 to 1.98 ng g(-1)d.w. BDE209 was the dominating congener in all sediment samples, indicating the pollution of PBDEs in the Daliao River Estuary mainly came from the use of deca-BDE commercial mixtures. The intrusion of sea waters promoted the deposition of the colloid-associated PBDEs in the estuary. There were significantly negative correlations between PBDE concentration in sediment with pH value and salinity in the bottom water. The higher river flow in the flood season (summer) obviously accelerated the transport of PBDEs, and thereby increased the risk of PBDE contamination to the deep ocean. Moreover, a positive correlation between TOC and PBDE distributions was observed, suggesting that TOC regulated the distributions of PBDEs in sediments of Daliao River Estuary.
[show abstract][hide abstract] ABSTRACT: Destruction of chlorinated aromatic compounds was carried out over mesoporous CuxMg1−xAl2O4 spinel oxides with x=0, 0.2, 0.5 and 0.8, which were prepared by the coprecipitation method. All the catalyst samples were characterized by means of X-ray diffraction (XRD), scanning electron microscopy (SEM) and nitrogen adsorption–desorption isotherms. The performances of these catalysts on the decomposition of hexachlorobenzene (HCB) and the catalytic formation of polychlorinated dibenzodioxin and dibenzofuran (PCDD/F) were evaluated in a closed system. The activities of these catalysts for dechlorination of HCB increased with increasing the magnesium content in them. The Cu0.2Mg0.8Al2O4 presented a higher catalytic activity for the hydrodechlorination of HCB than CuO, Al2O3, as well as the other CuxMg1−xAl2O4 spinels. More than 99% dechlorination efficiency of HCB was achieved at low temperatures (≤300°C) for a short time (30min) under air atmosphere over Cu0.2Mg0.8Al2O4 spinel. In comparison to air atmosphere, nitrogen atmosphere seemed to be more beneficial for the dechlorination of HCB with a dechlorination efficiency of 90% at 250°C for 10min. For the Cu0.2Mg0.8Al2O4 catalyst, the I-TEQ of PCDD/F formed was 4.5pg/g, far below the value of 15122.5pg/g on CuO. The Cu0.2Mg0.8Al2O4 catalyst inhibited the chlorination reaction and enhanced the dechlorination reaction. The catalytic behavior including catalytic dechlorination of HCB and catalytic formation of PCDD/F related to the physical characteristics of CuxMg1−xAl2O4 spinel was discussed.
[show abstract][hide abstract] ABSTRACT: Column sorption experiments were conducted at 330°C and 250°C to study the vapor-phase sorption of hexachlorobenzene (HCB) on two kinds of municipal solid waste (MSW) incinerator fly ashes, kaolinite, montmorillonite and activated carbon (AC). Both Freundlich equation and linear equation well fitted the sorption isotherms of HCB vapor on fly ashes and clay minerals at 330°C (r(2)>0.87), while the sorption isotherm of HCB vapor on AC at 330°C was in the shape of Brunauer type-II adsorption. Catalytic dechlorination of HCB was found to occur on the surface of fly ashes, and pentachlorobenzene was the only dechlorination product detected in the effluent gas. Increasing temperature decreased the sorption of HCB vapor on fly ashes, and promoted the catalytic dechlorination of HCB. On the assumption that the organic carbon and clay minerals in fly ash were derived from AC, kaolinite and montmorillonite, the relative contributions of these components to the apparent sorption capacity of fly ashes at 330°C were estimated. It was found that very small percentage of AC contributed the most to the apparent sorption of HCB vapor on fly ash. The sorption coefficient of HCB on montmorillonite at 250°C was 37 times higher than that at 330°C, suggesting montmorillonite could be a kind of low-cost sorbent to effectively reduce the emission of vapor-phase organochlorine compounds from MSW incinerators.
[show abstract][hide abstract] ABSTRACT: To achieve a higher efficiency of thermal remediation of soil contaminated with organic compounds, the retention mechanisms of organic compounds on thermally treated soil need to be understood adequately. In this study, a soil-column gas chromatography approach was developed to determine the soil-air partition coefficients (K(SA)) at 300 degrees C for a diverse set of nonionic organic compounds bearing many different functional groups; and the retention mechanisms of these organic compounds on two typical soils, isohumisols and ferralisols, were characterized using a polyparameter linear free energy relationship (pp-LFER). The K(SA) values (mL g(-1)) of typical volatile organic compounds (VOCs) with lower boiling points were <1.5 and in some cases even below 1.0, suggesting the rapid removal of VOCs from soils at 300 degrees C. Moreover, the K(SA) values were found to be a strong function of the soil-column temperature T (K), and be almost independent of the carrier-gas flow rate. Significant differences in molecular interactions were noted among various soil-solute pairs. The relative contributions of nonspecific van der Waals forces to the retention of test polar solutes were higher on isohumisols than on ferralisols. In contrast to the reported pp-LFER models for natural soils and soil components at normal environmental temperatures, our results suggest that elevated temperature remarkably reduces H-bond interactions between polar organic compounds and the soil matrix, thus allowing accelerated desorption of polar organic compounds from soils during thermal treatment.
Environmental Science and Technology 05/2010; 44(10):3677-82. · 5.26 Impact Factor
[show abstract][hide abstract] ABSTRACT: The contamination status of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) and polychlorinated biphenyls (PCBs) in the surface sediments of the middle and lower reaches of the Liaohe River, which is one of the most heavily polluted rivers in China, were investigated. The concentrations of PCDD/Fs and PCBs were in the range of 0.24-27.49 ng WHO-TEQ kg(-1) (average value: 3.01 ng WHO-TEQ kg(-1)) and 0.015-0.99 ng WHO-TEQ kg(-1) (average value: 0.33 WHO-TEQ kg(-1)), respectively. For most sediment samples, the pollution levels of PCDD/Fs and PCBs exceeded the interim sediment quality guidelines (ISQGs) established by the Canadian Council of Ministers of the Environment. The highest level of PCDD/F contamination was found at the Tai'an reach of the Liaohe River, whereas the highest level of PCB contamination was found at the Xinmin reach of the Liaohe River. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) were applied to the data set of sediment samples and to source-related samples from the literature. The results suggested that, PCDD/F contamination in most sediments of the middle and lower reaches of the Daliao River should mainly originated from the local combustion processes, including coal burning, agricultural straw open burning, iron ore sintering, cement production and secondary Al and Cu metallurgy. For the PCBs, the major source was identified as the usage of technical PCB products, especially Chinese No. 1 PCB.
[show abstract][hide abstract] ABSTRACT: Destruction of polychlorinated aromatic compounds was carried out over spinel-type catalysts XY2O4 (where X = Mg, Ca, Cu, Ni, Zn, and Y = Al, Fe). The catalysts were characterized by XRD, nitrogen adsorption-desorption isotherms and FTIR. The performance of these catalysts toward the decomposition of hexachlorobenzene (HCB) and octachlorodibenzo-p-dioxin (OCDD) was evaluated in a closed system. The spinel-type catalyst with mesoporous structure demonstrated high catalytic activity for the hydrodechlorination of polychlorinated aromatic compounds. Among them, the copper-aluminum spinel (CuAl2O4), specifically calcined at 600 degrees C, exhibited the best activity. More than 85% dechlorination efficiency of HCB and 99% decomposition of polychlorinated dibenzodioxin (PCDD) were achieved at 250 degrees C for 30 min over the above catalyst which was more effective than the corresponding metallic copper and copper oxide catalysts during the thermal degradation of polychlorinated aromatic compounds. The correlation of catalytic performance to structural characteristics is discussed based on the detailed characterization. The simple preparation procedure and reasonable cost of the spinel-type catalysts present a good potential for the thermal treatment of polychlorinated aromatic pollutants at lower temperatures.
Environmental Science and Technology 03/2010; 44(8):3079-84. · 5.26 Impact Factor
[show abstract][hide abstract] ABSTRACT: The metabolic responses of Arabidopsis thaliana to cadmium exposure was characterized in this study. A. thaliana was cultivated in medium contaminated with different cadmium concentrations (0, 5 and 50microM, respectively) for 2weeks. Metabolite analyses were performed using gas chromatography-mass spectrometry. More than 80 metabolites characterized by retention time indices and specific mass fragments were identified. The levels of carbohydrates, organic acids, amino acids, and other stress-responsive metabolites changed under cadmium stress. Treated plants showed increased levels of Ala, beta-ala, Pro, Ser, putrescine, Suc and other metabolites with compatible solute-like properties, notably 4-aminobutyric acid, glycerol, raffinose and trehalose, compared to control (untreated) plants. Studies indicated that concentrations of antioxidants (alfa-tocopherol, campesterol, beta-sitosterol and isoflavone) also increased significantly. These results confirm the important role of antioxidant defences in the mechanisms of plant-resistance to cadmium stress. Our results suggested that metabolic profiling is a powerful tool that can rapidly classify environmentally modified plants and simplify the process of cadmium-stress responses. These data will be helpful for better understanding of mechanisms of plant adaptation to cadmium stress at the metabolite level.
[show abstract][hide abstract] ABSTRACT: Stack gas samples from 14 domestic-made hospital waste incinerators (HWIs) in China were collected and analyzed for polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs). The waste burning capacities of the investigated HWIs ranged from 5 to 25 ton d(-1). The stack gas emissions of PCDD/Fs from HWIs exhibited a large variation (0.08-31.60 ng I-TEQNm(-3)). Nine incinerators had the emission levels below the current emission standard in China (0.5 ng I-TEQN m(-3)), while only two facilities exhibited emission levels below the European Union directive emission limit (0.1 ng I-TEQN m(-3)). For two plants, concentrations of PCDD/Fs in stack gas were above 10.0 ng I-TEQN m(-3). The emission factors of PCDD/Fs from the investigated HWIs were in the range of 0.78-473.97 microg I-TEQ ton(-1) medical wastes. It was estimated that 4.87 g I-TEQ of PCDD/Fs was annually released from HWIs to the atmosphere in China in 2006. Principal component analysis and hierarchical cluster analysis were applied to analyze the congener profiles of PCDD/Fs from HWIs in China.
[show abstract][hide abstract] ABSTRACT: Root uptake and subsequent translocation of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in 12 agricultural crops were comparatively investigated. All crop plants were exposed hydroponically to a mixture of three kinds of dioxin congeners over 4d. The root concentration factor (RCF) of dioxin showed a logarithmic correlation with extractable lipid content in plant root. On the assumption that the dioxin escaping via gas phase from nutrient solution in the closed container can evenly diffuse in the air and equally absorb onto the shoot tissues of the dioxin-exposed plant and their nearby blank control plant, the amount of translocated dioxin was estimated by subtracting dioxin content in the shoot tissues of the blank control plant from that of the dioxin-exposed plant, and then the transpiration stream concentration factor (TSCF) of dioxin was calculated. The TSCF values of PCDD/Fs largely varied according to the plant species, and the TSCF values of 2,4,8-TrCDF were a little higher than those for 1,3,6,8-TeCDD expect for zucchini. For 1,3,6,8-TeCDD, zucchini had the highest TSCF value of 0.0089, followed by pumpkin (0.0064) towel gourd (0.0027), and cucumber (0.0010), verifying plants of the genus Cucurbita have the higher abilities of dioxin translocation. The TSCF values of 1,3,6,8-TeCDD for wheat and sorghum were 0.0013 and 0.0012, respectively. For maize, soybean, rice, Chinese cabbage, tomato and garland chrysanthemum, translocation was an insignificant mechanism of dioxin contamination in shoot tissues.
[show abstract][hide abstract] ABSTRACT: Gas emission of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/Fs) from 19 commercial municipal solid waste (MSW) incinerators in China are investigated. The emission concentrations of PCDD/Fs were 0.042-2.461 ng TEQ Nm(-3) with an average value of 0.423 ng TEQ Nm(-3). The emissions of PCDD/Fs from 16 MSW incinerators were below the MEP regulation level (1.0 ng I-TEQ Nm(-3)), while only six systems have the dioxin emission levels below the limit established by the European Union Directive of 0.1 ng I-TEQ Nm(-3). The emission factors of PCDD/Fs from 19 MSW incinerators were calculated to be 0.169-10.72 microg TEQ ton(-1) MSW with an average value of 1.728 microg I-TEQ ton(-1) MSW. The total amount of PCDD/Fs emitted from MSW incinerators to the atmosphere in China was estimated to 19.64 g TEQ year(-1) in 2006.
[show abstract][hide abstract] ABSTRACT: The surface of spherical magnesium oxide has been modified with ethylene glycol in the presence of basic aqueous solution. Fourier transform infrared spectra have shown the presence of the graft group ethylene glycol on the surface of spherical magnesium oxide. In addition, this conclusion can also be drawn from appearance of new diffraction peak in X-ray diffraction pattern. Although magnesium oxide microspheres can directly react with ethylene glycol in 373 K for 24 h, owing to Lewis acid and alkali interaction, product derived is colloidal and difficult to filter so that their morphology is easy to be destroyed. However, in the presence of basic aqueous solution, their morphology still retains. The modified magnesium oxide illustrates a specific surface area of 55.9 m2/g and a narrow pore size distribution centered at 8.96 nm.
Materials Letters - MATER LETT. 01/2009; 63(17):1514-1516.
[show abstract][hide abstract] ABSTRACT: Three dimensional ordered graphitized mesoporous carbon GMC-6 (pore diameter 6 nm) and GMC-13 (pore diameter 13 nm), prepared by a nickel-catalyzed template-assisted method, were explored systematically for the construction of enzyme-based electrochemical biosensors. Comparative studies revealed that GMC-6 offer significant advantages over GMC-13 and graphitized multiwalled carbon nanotubes (CNTs) in facilitating the direct electron transfer of entrapped hemoglobin and improving the performance of fabricated biosensors. The possible factors that affect the biosensing performance of these carbon materials were evaluated comprehensively and comparatively based on the characterization of their physical parameters. The biosensor based on GMC-6 displayed excellent analytical performance over a wide linear range along with good stability and selectivity for the detection ofhydrogen peroxide. The “entrapment” immobilization mode and “interspace confinement effect” (by restraining the unfolding or conformational change of enzyme molecules from inactivation) provided by GMC can result in pore-size-dependent enzymatic stability and bioactivity, which might be a crucial factor for the superior biosensing performance of GMC-6 to that of CNTs and GMC-13. Graphitized ordered mesoporous carbons with good pore size matching for enzymes proved to be a promising electrochemical biosensing platform.
Journal of Materials Chemistry 01/2009; 19(27). · 5.97 Impact Factor