Jürgen Lademann

Charité Universitätsmedizin Berlin, Berlín, Berlin, Germany

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Publications (412)980.32 Total impact

  • Journal der Deutschen Dermatologischen Gesellschaft 06/2013; 11(10). DOI:10.1111/ddg.12144 · 1.40 Impact Factor
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    ABSTRACT: Herd health programs for the maintenance of welfare and productivity in cattle need efficient tools for monitoring the health of individual animals. Recent reports demonstrate that the oxidative status is related to various stress conditions in dairy cows. Biomarkers, among other carotenoids, could serve as indicators of stress originating from the environment (e.g., heat stress or sun radiation) or from the animal itself (e.g., disease). To date, only invasive in vitro tests are available to assess the oxidative status in cattle. The present study compares the results of optical noninvasive in vivo measurements of dermal carotenoids in cattle udder skin using an LED-based miniaturized spectroscopic system (MSS) with those obtained by photometric analysis of beta carotene in whole blood samples using a portable device. Correlations between the concentrations of dermal and blood carotenoids were calculated under consideration of the nutritional status of the animals. Significant correlation (R = 0.86) was found for cattle with a moderate to obese body condition. Thus, the blood and skin concentrations of the marker substance beta carotene are comparable under stable stress conditions of the cattle. This demonstrates that the MSS is suitable for noninvasive assessment of dermal carotenoid concentrations in cattle.
    Journal of Biomedical Optics 06/2013; 18(6):061219. DOI:10.1117/1.JBO.18.6.061219 · 2.75 Impact Factor
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    ABSTRACT: The standard diagnostic procedure for basal cell carcinoma (BCC) is invasive tissue biopsy with time-consuming histological examination. To reduce the number of biopsies, noninvasive optical methods have been developed providing high-resolution skin examination. We present direct comparison of a reflectance confocal microscope (RLSM) and a multiphoton tomograph (MPT) for BCC diagnosis. Both systems are applied to nine patients prior to surgery, and the results are analyzed, including histological results. Both systems prove suitable for detecting typical characteristics of BCC in various stages. The RLSM allows large horizontal overview images to be obtained, enabling the investigator to find the regions of interest quickly, e.g., BCC nests. Elongated cells and palisading structures are easily recognized using both methods. Due to the higher resolution, changes in nucleus diameter or cytoplasm could be visualized with the MPT. Therefore, the nucleus diameter, nucleus/cytoplasm ratio, and cell density are estimated for normal and BCC cells using the MPT. The nucleus of elongated BCC cells is significantly longer than other measured normal skin cells, whereas the cell density and nucleus/cytoplasm ratio of BCC cannot be significantly distinguished from granular cells.
    Journal of Biomedical Optics 06/2013; 18(6):61229. DOI:10.1117/1.JBO.18.6.061229 · 2.75 Impact Factor
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    ABSTRACT: The results of the risk assessment of the tissue-tolerable plasma (TTP) jet kINPen med® and first results of pilot clinical studies are presented. Producing an atmospheric pressure plasma, this plasma jet entails no risk for humans in terms of temperature increase, UV radiation or free radical formation by the plasma. The antiseptic efficacy in vitro on porcine skin and in vivo on human skin was compared to that of octenidine. TTP could significantly reduce the bacterial load in comparison to untreated skin. However, the slightly reduced antiseptic properties of TTP are attributed to the current parameter set-up and technical limitations.
    06/2013; 1(1):5–10. DOI:10.1016/j.cpme.2013.01.001
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    ABSTRACT: Carotenoids are important substances for human skin due to their powerful antioxidant properties in reaction of neutralization of free radicals and especially reactive oxygen species, including singlet oxygen. Concentration of carotenoids in the skin could mirror the current redox status of the skin and should be investigated in vivo. Optical methods are ideally suited for determination of carotenoids in mammalian skin in vivo as they are both noninvasive and quick. Four different optical methods could be used for in vivo measurement of carotenoids in the human or animal skin: (1) resonance Raman spectroscopy; (2) Raman microscopy; (3) reflection spectroscopy; (4) skin color measurements. The advantages, shortcomings, and limitations of the above-mentioned optical methods are discussed.
    Journal of Biomedical Optics 06/2013; 18(6):61230. DOI:10.1117/1.JBO.18.6.061230 · 2.75 Impact Factor
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    ABSTRACT: Vitamin C is a potent radical scavenger and a physiological part of the antioxidant system in human skin. The aim of this study was to measure changes in the radical-scavenging activity of human skin in vivo due to supplementation with different doses of vitamin C and at different time points. Therefore, 33 volunteers were supplemented with vitamin C or placebo for 4 weeks. The skin radical-scavenging activity was measured with electron paramagnetic resonance spectroscopy. After 4 weeks, the intake of 100 mg vitamin C/day resulted in a significant increase in the radical-scavenging activity by 22%. Intake of 180 mg/day even resulted in a significant increase of 37%. No changes were found in the placebo group. A part of the study population was additionally measured after 2 weeks: in this group radical scavenging had already reached maximal activity after 2 weeks. In conclusion, orally administered vitamin C increases the radical-scavenging activity of the skin. The effect occurs fast and is enhanced with higher doses of vitamin C.
    Skin pharmacology and physiology 05/2013; 26(3):147-154. DOI:10.1159/000350833 · 1.96 Impact Factor
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    ABSTRACT: This contribution presents an alternative setup for analytical micro-spectroscopy by means of coherent anti-Stokes Raman scattering (CARS). Instead of using separate detectors for forward- and epi-scattered photons, this setup enhances the comparability of the signals by coupling them into a multimodal optical fiber and employing a fiber optical switch for selection of the signal source at a single detector. To facilitate measurements at different spectral positions, which might be hampered by laser power fluctuations, a feedback loop is introduced into the pump beam to ensure stable and pre-defined laser power levels. The scheme of the microscope and the control software is presented. Its potential is evaluated by recording spectral data of model analytes as well as images of human skin.
    Laser Physics Letters 05/2013; 10(6):065605. DOI:10.1088/1612-2011/10/6/065605 · 2.96 Impact Factor
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    ABSTRACT: Dendritic cells (DCs) are key activators of cellular immune responses through their capacity to induce naïve T cells and sustained effector T cell responses. This capacity is a function of their superior efficiency of antigen presentation via MHC class I and class II molecules, and the expression of co-stimulatory cell surface molecules and cytokines. Maturation of DCs is induced by microbial factors via pattern recognition receptors such as Toll-like receptors, pro-inflammatory cytokines or cognate interaction with CD4(+) T cells. Here we show that, unexpectedly, the PanDR helper T cell epitope PADRE, a generic T helper cell antigen presented by a large fraction of HLA-DR alleles, when delivered in particle-bound form induced maturation of human DCs. The DCs that received the particle-bound PADRE displayed all features of fully mature DCs, such as high expression of the co-stimulatory molecules CD80, CD86, CD83, the MHC-II molecule HLA-DR, secretion of high levels of the biologically active IL-12 (IL-12p70) and induction of vigorous proliferation of naïve CD4(+) T cells. Furthermore, the maturation of DCs induced by particle-bound PADRE was shown to involve sphingosine kinase, calcium signaling from internal sources and downstream signaling through the MAP kinase and the p72syk pathways, and finally activation of the transcription factor NF-κB. Based on our findings, we propose that particle-bound PADRE may be used as a DC activator in DC-based vaccines.
    PLoS ONE 05/2013; 8(5):e63039. DOI:10.1371/journal.pone.0063039 · 3.53 Impact Factor
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    ABSTRACT: The formation of radicals plays an important role in the development of atopic eczema or barrier-disrupted skin. We evaluated the radical scavenging effect of a cream containing a Hypericum perforatum extract rich in hyperforin in a double-blind placebo-controlled study on 11 healthy volunteers. Electron paramagnetic resonance spectroscopy was applied to determine radical formation during VIS/NIR irradiation of the inner forearm. The results were compared to ex vivo investigations on excised porcine ear skin after a single application of the creams. The non-treated skin was measured as control. The absolute values and the kinetics are not comparable for ex vivo and in vivo radical formation. Whereas in vivo, the radical production decreases with time, it remains stable ex vivo over the investigated timescale. Nevertheless, ex vivo methods could be developed to estimate the protection efficiency of creams. In vivo as well as ex vivo, the radical formation could be reduced by almost 80% when applying the hyperforin-rich cream onto the skin, whereas placebo resulted in about 60%. In vivo, a daylong protection effect could be validated after a 4-week application time of the cream indicating that a regular application is necessary to obtain the full effect.
    Experimental Dermatology 05/2013; 22(5):354-7. DOI:10.1111/exd.12124 · 4.12 Impact Factor
  • Alexa Patzelt, Juergen Lademann
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    ABSTRACT: Introduction: The optimization of drug delivery to and via the hair follicles is gaining more and more importance as it has been recognized that the hair follicles are an interesting target site for topical applications. They are closely surrounded by capillaries and antigen-presenting cells, are associated with the sebaceous glands and are the host of stem cells in the bulge region of the hair follicle. Areas covered: The present review shortly summarizes the complexity of the structure, biology and functions of the hair follicle and presents the models and methods suitable to investigate follicular penetration. Drug delivery to hair follicles was clearly shown to be dependent on the physicochemical properties of the applied substances and vehicles as well as on the activity status, size and density of the hair follicles. Especially particulate substances were demonstrated to be proficient drug carriers into the hair follicles, whereas dependent data for transfollicular penetration into the deeper viable skin layers could only be found for non-particulate substances which then, however, received rapid access to the circulation when the follicular pathway was accessible. Expert opinion: Promising concepts to optimize hair follicle delivery and to beneficially utilize particulate substances for efficient follicular drug delivery are the application of external or internal stimuli for controlled drug release from the particles such as the combined application with protease or the usage of gold nanoparticles in combination with near-infrared irradiation.
    Expert Opinion on Drug Delivery 03/2013; DOI:10.1517/17425247.2013.776038 · 4.12 Impact Factor
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    ABSTRACT: This review considers qualifying standards of wound management, explains the hypothesis for the applicability of tissue tolerable plasma (TTP) and lists the TTP effects relevant for wound healing. TTP fulfils these requirements in-vitro and in-vivo together with its demonstrated good tissue tolerability. Cases of treatment of chronic wounds in pets are presented which healed by TTP in combination with polihexanide or octenidine within 3 to 24 weeks. It may be concluded that TTP is a new option for the treatment of chronic wounds.
    03/2013; DOI:10.1016/j.cpme.2013.03.002
  • Zeitschrift für Phytotherapie 03/2013; 34(S 01). DOI:10.1055/s-0033-1338213
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    ABSTRACT: Microscopic imaging based on multiphoton fluorescence, second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) imaging has been realized in one common platform which is appropriate for use in hospitals. The different optical modalities non-invasively provide in vivo images from human skin with subcellular resolution, at different depths based on endogenous fluorescent, SHG-active molecules as well as non-fluorescent molecules with vibrational resonances at 2845 cm-1, in particular lipids. An overview of the system employing a Ti:sapphire laser and photonic crystal fiber to generate the excitation light as well as several imaging examples are presented.
    Conference on Multiphoton Microscopy in the Biomedical Sciences XIII; 02/2013
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    ABSTRACT: Needle-free vaccination, for reasons of safety, economy and convenience, is a central goal in vaccine development but also needs to meet the immunological requirements for efficient induction of prophylactic and therapeutic immune responses. Combining the principles for non-invasive delivery to dendritic cells through skin and the immunological principles of cell-mediated immunity we developed microparticle-based topical vaccines. We show here that the microparticles are efficient carriers for coordinated delivery of the essential vaccine constituents to dendritic cells for cross-presentation of the antigens and stimulation of T cell responses. When applied to the skin, the microparticles penetrates into hair follicles and target the resident dendritic cells, the immunologically most potent cells and site for induction of efficient immune responses. The microparticle vaccine principle can be applied to different antigen formats such as peptides and proteins, or nucleic acids coding for the antigens.Journal of Investigative Dermatology accepted article preview online, 20 February 2013; doi:10.1038/jid.2013.79.
    Journal of Investigative Dermatology 02/2013; DOI:10.1038/jid.2013.79 · 6.37 Impact Factor
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    ABSTRACT: Hair follicles represent reservoirs for localized drug therapy and transport pathways for systemic drug delivery. This study describes an approach towards developing a novel procedure for quantifying topically applied substances located in the hair follicles of porcine ear skin, a model for human in vivo skin, using a fluorescent dye. Approximately 5% of the topically applied dye was recovered from the hair follicles, which is in accordance with a previous study.
    Experimental Dermatology 02/2013; DOI:10.1111/exd.12113 · 4.12 Impact Factor
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    ABSTRACT: We combined two-photon fluorescence and coherent anti-Stokes Raman scattering (CARS) imaging in a clinical hybrid multiphoton tomograph for in vivo imaging of human skin. The clinically approved TPEF/CARS system provides simultaneous imaging of endogenous fluorophores and non-fluorescent lipids. The Stokes laser for the two-beam configuration of CARS is based on spectral broadening of femtosecond laser pulses in a photonic crystal fiber (PCF). We report on the highly flexible medical TPEF/CARS tomograph MPTflex®-CARS with an articulated arm and first in vivo measurements on human skin.
    Proceedings of SPIE - The International Society for Optical Engineering 02/2013; DOI:10.1117/12.2001855 · 0.20 Impact Factor
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    ABSTRACT: We report on in vivo coherent anti-Stokes Raman scattering spectroscopy (CARS), two-photon fluorescence and second-harmonic-generation imaging on human skin with a novel multimodal clinical CARS/multiphoton tomograph. CARS imaging is realized by a combination of femtosecond pulses with broadband continuum pulses generated by a photonic crystal fiber. The images reveal the microscopic distribution of (i) non-fluorescent lipids, (ii) endogenous fluorophores and (iii) the collagen network inside the human skin in vivo with subcellular resolution. Examples of healthy as well as cancer-affected skin are presented.
    Laser Physics Letters 01/2013; 10(2):025604. DOI:10.1088/1612-2011/10/2/025604 · 2.96 Impact Factor
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    ABSTRACT: In this study, we compared the UV-protective abilities of the europium complex compared to titanium dioxide, which represents the most common physical filter for ultraviolet light in the broad-band spectral range. The UV absorption and light transformative capacities of the europium complex were evaluated using a spectrometer with a double-integrating sphere showing that the europium complex does not only absorb and reflect UV light, but transforms it into red and infrared light. It was found that the europium complex binds to the surface of Jurkat cells in vitro. Cells incubated with the europium complex showed a significantly higher viability after UVA and UVB irradiation as compared to untreated cells and cells incubated with titanium dioxide pointing out its photoprotective properties. The europium complex and titanium dioxide show similar penetration capacities into the stratum corneum as tested in human and porcine skin using tape stripping analysis. The europium complex has proved to be an efficient UV filter with a low cyto- and phototoxic profile and therefore represents a potential candidate for use in sunscreen formulations.
    Skin pharmacology and physiology 01/2013; 26(2):76-84. DOI:10.1159/000345976 · 1.96 Impact Factor
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    ABSTRACT: The effect of tissue-tolerable plasma (TTP) on inflammatory diseases such as psoriasis was investigated. Three plaques of six psoriatic patients were subjected to different treatments (A: TTP, brine baths (BB)+5% salicylic acid ointment (SAO); B: BB+SAO; C: BB, UV irradiation, SAO+dithranol). While redness and infiltration was reduced in groups A and C, scaling was reduced in group C. TTP temporarily reduced the bacterial colonization on the skin lesions. In summary, the treatment of psoriatic plaques with TTP showed no significant advantage over conventional therapies.
    01/2013; DOI:10.1016/j.cpme.2013.11.002
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Publication Stats

7k Citations
980.32 Total Impact Points

Institutions

  • 2000–2015
    • Charité Universitätsmedizin Berlin
      • Department of Dermatology, Venerology and Allergology
      Berlín, Berlin, Germany
  • 2014
    • University of Greifswald
      • Institute of Hygiene and Environmental Medicine
      Griefswald, Mecklenburg-Vorpommern, Germany
  • 2003–2012
    • Freie Universität Berlin
      • • Ruminant and Swine Clinic
      • • Institute of Pharmacy
      • • Division of Physical and Theoretical Chemistry
      Berlín, Berlin, Germany
  • 1999–2012
    • Humboldt-Universität zu Berlin
      • Department of Chemistry
      Berlín, Berlin, Germany
  • 2011
    • Universität des Saarlandes
      • Biophotonik und Lasertechnologie
      Saarbrücken, Saarland, Germany
  • 2007
    • Dupont
      Delaware, Ohio, United States
  • 1997
    • Universität der Bundeswehr München
      • Institut für Physik
      Neubiberg, Bavaria, Germany