Jun Lin

Zhejiang University, Hang-hsien, Zhejiang Sheng, China

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Publications (64)155.74 Total impact

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    ABSTRACT: Light-induced cell detachment on the substrate of TiO2 nanodots has been proved to be a safe and attractive method for cell harvesting in tissue engineering. In order to improve the cell release efficiency, bioinert platinum with excellent charge transport loading in biocompatible graphite was prepared. 20 wt.% Pt/C powder showing excellent electrical, chemical and bioactive properties was incorporated to a TiO2 system. We investigated the biocompatibility of Pt/C–TiO2 composite films with different amounts of Pt/C with C/tetrabutyl titanate molar ratio varying from 0 to 0.42. The adhesion and proliferation rates of pre-osteoblastic MC3T3-E1 cells (mouse calvaria-derived) on the films prepared with the highest C/TiO2 molar ratio are higher than that of the pure TiO2 and other films, indicating a Pt/C dose-dependent effect. It is shown that the detachment efficiency of MC3T3-E1 cells depends on Pt/C amount and UV illumination time. Moreover, films prepared with C/tetrabutyl titanate molar ratio of 0.42 showed 93% cell detachment ratio within 5 min of UV illumination, while bare TiO2 films only reached 75%, indicating an improved cell release efficiency by the incorporation of Pt/C. However, an interesting phenomenon was found that longer illumination time showed lower detachment ratios to some extent, which is contrary to our previous work. It is believed that this work presents the improved cell detachment efficiency by the incorporation of Pt/C.
    Thin Solid Films 06/2015; 584. DOI:10.1016/j.tsf.2014.11.046 · 1.87 Impact Factor
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    ABSTRACT: Europium (Eu) doped TiO2 nanodots film were prepared through a phase-separation-induced self-assembly method. Eu was doped to impart the nanodots with luminescence property so that the protein adsorption could be in situ characterized quantitatively. Bovine serum albumin (BSA) was adsorbed on the surface of Eu-doped titanium nanodots films. It was found that the photo luminescence intensity at 616 nm decreased with the increase of BSA adsorption time. Also, Eu-doped TiO2 nanodots films showed good biocompatibility. These results suggested that Eu-doped TiO2 nanodots films could provide a feasible in situ way to evaluate protein adsorption if prepared on the surface of bioimplants.
    Thin Solid Films 01/2015; 584. DOI:10.1016/j.tsf.2015.01.003 · 1.87 Impact Factor
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    ABSTRACT: In this study, the influence of restoration type, symmetry, and color on the perception of dental appearance was evaluated. An esthetic questionnaire was completed by 29 patients before and after esthetic rehabilitation. In addition, 94 dentists from four countries (Germany, the United Kingdom [UK], China, and Switzerland) evaluated the influence of the above factors using before-and-after rehabilitation pictures. The most invasive treatment was recommended by Chinese dentists, while German, Swiss, and UK dentists recommended comparable treatment options. As for restorative symmetry, restoration type, and color, significant differences could be found among and within the dentists of the four countries (P ± .05).
    The International journal of prosthodontics 01/2015; 28(1):60-4. DOI:10.11607/ijp.4005 · 1.19 Impact Factor
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    ABSTRACT: Objective To observe efficacy following methotrexate (MTX) management in women with placenta accreta. Methods Twenty-four stable patients with placenta accreta were treated with MTX. Beta-hCG values, vascular indices of the residual placenta, and other clinical characteristics were collected prospectively and were compared between the success and failure groups. Results After MTX management, the residual placentas were expulsed spontaneously in 33.3 % of the patients. This was done through dilatation and curettage (D & C) in 45.8 % of the patients. The residuals in the uterine wall were completely absorbed within 5.7 months. In the patients who were successfully treated with MTX, their beta-hCG values and vascular indices of the placentas decreased faster than those of failure patients (P Conclusions MTX management, when the beta-hCG value and vascular indices of placenta decreased significantly, is a conservative option for a stable patient with placenta accreta in China. 3D power Doppler ultrasound should be utilized for the follow-up of this condition.
    Archives of Gynecology and Obstetrics 12/2014; DOI:10.1007/s00404-014-3573-1 · 1.28 Impact Factor
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    ABSTRACT: Design of an implant surface with appropriate nanostructures has been considered to be an effective way to promote osteoblast cell growth and osseointegration. In this work, TiO2 nanorods were integrated into TiO2 nanodot films by hydrothermal growth to engineer a nanostructured surface composed of two nano-structural elements. The surface microstructure of the films depended on the integration degree. Low integration led to a surface nanostructure with exposure of the two nano-structural elements, and high integration resulted in a similar surface to a nanorod film. The nanorod integration had an obvious influence on pre-osteoblast cell responses and accelerated the cellular osteogenesis. Two factors of the integrated nano-structural element and the changed surface topology are believed to be responsible for enhancing cellular responses. The former is considered to play a more significant role because the nanorods tended to adsorb human fibronectin preferentially so as to begin differentiation to osteogenesis earlier. We anticipate this work may assist to gain insight into understanding of the interactions between nanostructured surfaces with cells. Copyright © 2014 Elsevier B.V. All rights reserved.
    Colloids and surfaces B: Biointerfaces 12/2014; 126. DOI:10.1016/j.colsurfb.2014.12.002 · 4.29 Impact Factor
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    ABSTRACT: For orthopedic implant coatings, excellent biological performance and anti-infective property have been both increasingly required for implantation surgery. In this work, TiO2 nanorod films with good cellular responses and mesoporous bioactive glass (MBG) with good drug loading and release ability were adopted, and MBG-incorporated TiO2 nanorod films were prepared by the sol–gel technique. The effects of TiO2 nanorod density and MBG composition on the formation of the incorporated films were investigated. The results show that T-MBG (SiO2/CaO/P2O5/TiO2 = 80/5/5/10) tightly cohered to TiO2 nanorods in the films, and the degree of T-MBG incorporation ranged from about 100 nm to 300 nm of exposed TiO2 nanorods in height. T-MBG incorporation had a positive role in the cytocompatibility of the films. Importantly, T-MBG incorporation improved greatly the drug release behavior of the TiO2 nanorod films. The present work provides an approach to build organized nanostructure films on implant surfaces with satisfactory biological performance and essential anti-infective ability.
    Thin Solid Films 12/2014; 584. DOI:10.1016/j.tsf.2014.12.034 · 1.87 Impact Factor
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    ABSTRACT: In this study, we investigated the differences between amorphous and anatase TiO2 at the biomolecular level which could explain differences in the osteoblast response on these surfaces. The number of surface hydroxyl groups in the TiOHT form on amorphous and anatase TiO2 was found to be the most important factor, resulting in adsorption of bovine serum albumin as a monolayer on amorphous TiO2 nanodots but as a multilayer on anatase TiO2 nanodots. The reason for this is that the presence of more TiOHT groups on amorphous TiO2 nanodots attracts more -NH3(+) groups on BSA molecules, causing the conformation of surface-bound BSA molecules to differ from those adsorbed on anatase TiO2 nanodots. Fibronectin which is subsequently adsorbed on anatase TiO2 nanodots then retains a more active conformation for osteoblast adhesion and mineralization.
    Colloids and surfaces B: Biointerfaces 08/2014; 123. DOI:10.1016/j.colsurfb.2014.08.030 · 4.29 Impact Factor
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    ABSTRACT: The aim of this study is to evaluate the influence of Tooth Mousse (TM) application, smear layer removal, and storage time on resin-dentin microtensile bond strength (µTBS). Dentin specimens were divided into two groups: (1) smear layer covered; (2) smear layer removed using 15% EDTA for 90 s. In each group, half the specimens were treated once with TM for 60 min. After bonding procedures using a two-step self-etching adhesive (Clearfil SE Bond (CSE); Kuraray Medical, Tokyo, Japan), an all-in-one adhesive (G-Bond (GB); GC Corp, Tokyo, Japan), and a total-etch adhesive (Adper Single Bond 2 (SB); 3M ESPE, St. Paul, MN, USA), the specimens were stored for 3 d or 6 months in deionized water at 37 °C, and µTBS was tested and analyzed. With the exception of SB (no TM application) and GB, the μTBS was significantly increased for CSE and SB using EDTA pre-conditioning and 3 d of storage (P≤0.001). Bond strength of GB decreased significantly when using EDTA (3 d storage, P<0.05). TM application only increased the μTBS of GB (no EDTA) and SB (with EDTA) after 3 d (P≤0.02). Comparing the adhesives after 3 d of storage, CSE exhibited the greatest μTBS values followed by GB and SB (P≤0.02). The factors of adhesive, EDTA, and TM did not show any significant impact on μTBS when specimens were stored for 6 months (P>0.05). The additional application of TM and EDTA for cavity preparation seems only to have a short-term effect, and no influence on µTBS of dentin bonds after a period of 6 months.
    Journal of Zhejiang University SCIENCE B 07/2014; 15(7):649-60. DOI:10.1631/jzus.B1300216 · 1.29 Impact Factor
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    ABSTRACT: Loading of an appropriate amount of rhBMP-2 and avoiding its “burst-release” are key challenges for upgrading the biological performance of thin bioactive coatings on metal implants. In this study, we adopted incorporation of chitosan nanospheres into thin mineralized collagen coatings to enhance rhBMP-2 loading and improve releasing behavior based on the good affinity of chitosan for proteins and the large surface area of nanospheres. We realized the incorporation process by electrophoretic injection. Using chitosan nanospheres, we were able to increase the rhBMP-2 loading amount in the thin coating by 2.7-fold (from 446 ng cm−2 to 1186 ng cm−2), and showed that the rhBMP-2 exhibited sustained release behavior. MC3T3-E1 cells cultured on the rhBMP-2/chitosan nanosphere-incorporated thin coatings (Col/Cs/BMP) showed good cell attachment and proliferative behavior, and high levels of differentiation and mineralization. In in vivo tests, spiral CT analysis and histological observations demonstrated that Col/Cs/BMP coatings on metal implants were able to increase bone density and accelerate new bone growth after implantation for 4 to 8 weeks, and the boundary between host bones and new bones disappeared after implantation for 8 weeks. The pull-out tests further confirmed that the Col/Cs/BMP coatings could significantly enhance osseointegration. The present results indicate that incorporation of chitosan nanospheres into thin coatings is an effective way to enhance the loading amount properly, improve the release behavior of rhBMP-2 and finally accelerate the osseointegration process.
    06/2014; 2(28). DOI:10.1039/C4TB00404C
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    ABSTRACT: Peritoneal dialysis plays a crucial role in the integrated care of patients with end-stage renal disease (ESRD). In this paper, we retrospectively analyzed the quality indicators of peritoneal dialysis (PD) in 712 patients from our center who underwent PD between 2004 and 2011. In 43% of patients, follow-up was undertaken every 3 months at our outpatient department, and 54% patients were followed up by both our hospital and other local hospitals. The patient survival rate at 1, 3 and 5 years was 96.3%, 85.4% and 76.2%, respectively. The technique survival (excludes death/transplantation) at 1, 3 and 5 years was 95.1%, 87.7% and 79.6%, respectively. Fluid overload occurred in 29.2% of patients and was one of the major reasons for discontinuing PD. The peritonitis rate in our center decreased to 0.16 episodes/year in 2011. In addition, since our center is one of the largest integrated-treatment centers for ESRD in China, we have developed a multilevel care program in Zhejiang Province, which resulted in rapid growth of PD in our province in recent years.
    Peritoneal dialysis international: journal of the International Society for Peritoneal Dialysis 06/2014; 34(Supplement 2):S55-S58. DOI:10.3747/pdi.2013.00124 · 2.20 Impact Factor
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    ABSTRACT: The modulating attributes of steroid receptor RNA activator protein (SRAP) on steroid receptors have been shown in some types of tumor cells. There is compelling evidence to suggest that this molecule may play a critical role in the development of the tumor. However, little has been reported on its expression in endometriosis associated ovarian clear cell carcinoma (EAOCCC). In order to investigate the role of SRAP and estrogen receptors (ERs) in EAOCCC, we have analyzed the distribution of these proteins in the malignant transformation tissues and endometrioma tissues by immunohistochemistry. Our results revealed that the positive ratio of ER-β expression was gradually reduced during the malignant transformation from endometriosis to atypical endometriosis to clear cell carcinoma. Conversely, during the process, a gradual increase in SRAP expression was observed. Furthermore, there is a negative relationship between the expressions of these two molecules. Overall an increase in SRAP and a reduction in ER-β expression might be associated with malignant transformation of EAOCCC.
    Acta histochemica 04/2014; DOI:10.1016/j.acthis.2014.02.007 · 1.76 Impact Factor
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    ABSTRACT: Abnormal endometrial expression of CC-chemokine receptor-1 (CCR1) may play a role in the pathogenesis of endometriosis. Adenomyosis, also called endometriosis interna, occurs when the endometrium invades the myometrium. The objective of this study was to determine CCR1 expression in endometrium in women with adenomyosis as compared to women without adenomyosis. We evaluated endometrial mRNA and protein expression in women with and without adenomyosis using quantitative polymerase chain reaction (PCR), immunohistochemical staining and western blot analysis, respectively. We detected CCR1-immunoreactive expression in endometrium in all women with and without adenomyosis. CCR1-immunoreactive staining in endometrial cells was significantly higher in women with adenomyosis (4.89±1.06) compared to those without adenomyosis (2.21±1.16, P⟨0.001). Women with adenomyosis had higher levels of CCR1 mRNA in endometrium compared to women without adenomyosis (P⟨0.05). CCR1 protein levels in endometrium were significantly higher in women with adenomyosis (1.66±0.79) compared to women without adenomyosis (0.56±0.13, P⟨0.001), and positively correlated with the severity of dysmenorrhea (r=0.87, P⟨0.001). These results suggest that increased CC-chemokine receptor expression may play a role in the pathogenesis of adenomyosis.
    Histology and histopathology 03/2014; · 2.24 Impact Factor
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    ABSTRACT: To explore the effects of exogenous suppressor of cytokine signaling 3 (SOCS3) in Janus Kinase/Signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathways and examine the relationship between exogenous SOCS3 and cell proliferation and apoptosis in ectopic endometrial stromal cells. Ectopic endometrial stromal cells from patients with histopathologically confirmed endometriosis were primarily cultured in vitro. Vimentin staining was used to identify the purity of endometrial stromal cells. Lentiviru containing SOCS3 and GFP (green fluorescent protein) recombinant (LV-SOCS3-GFP) or only GFP gene (LV-GFP) was used to transfect two groups of ectopic endometrial stromal cells from the same patient respectively. They were divided into experimental (EG) and negative (NC) control. The transfection efficiency of lentivirus was measured by GFP fluorescence expression under microscope. And the expressions of SOCS3, STAT3 and JAK2 mRNA and protein were analyzed by real-time polymerase chain reaction (PCR) and Western blot respectively. And phosphorylated JAK2 and STAT3 proteins (p-STAT3 and p-JAK2) were also analyzed by Western blot. Cell cycles and apoptosis were detected by flow cytometry. The transfection efficiency of lentivirus was over 80% at 72 h post-transfection. Compared with NC, EG showed a higher expression of SOCS3 mRNA (P < 0.001). SOCS3 protein level of EG was higher than NC while p-STAT3 protein of EG obviously lower than NC. STAT3, JAK2 and p-JAK2 proteins showed no difference between two groups. The percentage of apoptotic cells in EG was higher remarkably than NC (22.0 ± 1.3)% vs (4.4 ± 1.3)%, P < 0.01. Moreover, EG cells showed a G0/G1 arrest compared with NC (87.0 ± 3.7)% vs (76.0 ± 3.7)%, P < 0.05. Exogenous SOCS3 induces cell apoptosis and inhibits cell proliferation in ectopic endometrial stromal cells.
    Zhonghua yi xue za zhi 01/2014; 94(1):55-8.
  • International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics 12/2013; 124(3). DOI:10.1016/j.ijgo.2013.10.002 · 1.56 Impact Factor
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    ABSTRACT: We investigated the use of TiO2 nanorod films to construct an instructive microenvironment for regulation of biological activity. Rutile TiO2 nanorod films were fabricated via a hydrothermal method. Heat treatment was used to adjust the atomic arrangement order degree of the nanorod surfaces. The influence of changes in the nanorod surface on the films was characterized. The results show that heated nanorod films were well crystallized and the nanorod surfaces had a regular atomic arrangement. Unheated nanorod films had a higher contact angle and lower sensitivity to UV illumination, and showed good capacity for protein adsorption at an early stage, which may lead to better cytocompatibility. The results provide an insight into construction of an appropriate microenvironment for interaction between biomaterials and cells.
    Thin Solid Films 10/2013; 544:285-290. DOI:10.1016/j.tsf.2013.03.102 · 1.87 Impact Factor
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    ABSTRACT: Hydroxyapatite (HA)/ZnO-nanorod composite coatings were fabricated on Si substrates in this work. ZnO nanorods were firstly grown on the substrate through a hydrothermal method, and then CaP precursor solution was spin-coated on the surface and fired to form HA/ZnO-nanorod composite coatings. The results showed that ZnO nanorods were grown on substrates and hydroxyapatite was deposited between nanorods, leading to a composite coating. The surface wettability of such coating could be adjusted through ultraviolet treatment, from rather hydrophobic to hydrophilic. Such coatings showed different protein adsorption property from both ZnO nanorod coating and HA coating. Also, a desirable Zn ion release ability was observed. Such coatings could be potentially applied as bioactive coatings on metallic bioimplant surface.
    Thin Solid Films 10/2013; 544:260-264. DOI:10.1016/j.tsf.2013.03.108 · 1.87 Impact Factor
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    ABSTRACT: Titanium dioxide (TiO2) nanorods and calcium phosphate (CaP) composite coatings were prepared and characterized. TiO2 nanorod films were grown on titanium substrates via a hydrothermal method. Then, calcium phosphate was deposited into TiO2 nanorod films electrochemically in an aqueous electrolyte containing calcium and phosphorus species. As a result, CaP/TiO2 nanorod coatings were obtained. The amount of calcium phosphate deposited could be controlled through varying temperature, voltage and duration. It was found that both the presence of calcium phosphate and UV treatment could affect the surface wettability of the composite coatings. A bovine serum albumin adsorption test showed that the protein adsorption ability of the composite coatings was also improved with the presence of calcium phosphate and UV treatment. Moreover, the presence of calcium phosphate could promote the deposition of bone-likeminerals. It is suggested that such coatings may exhibit promising initial cellular responses and thus be a good alternate for bioactive coatings.
    Thin Solid Films 10/2013; 544:206-211. DOI:10.1016/j.tsf.2013.03.120 · 1.87 Impact Factor
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    ABSTRACT: The selective sorption of pentachlorophenol (PCP) in a multiple solute system provides the possibility of using the cetyltrimethylammonium bromide (CTMAB)/bentonite one-step process as a promising technique for hydrophobic organics removal from wastewater. The PCP sorption mechanism, especially the effects of coexisting solutes, was studied. Compared to raw bentonite and conventional CTMAB modified organobentonite, the one-step process exhibited higher PCP removal efficiency. In a single solute system, the PCP sorption isotherm implied that the dominating sorption mechanism is a partition mechanism. The logarithms of sorption coefficients (Kd) for different organics are linearly related to the logarithm of their octanol–water partition coefficients (Kow). In a multiple solutes system, phenol and 2,4-dichlorophenol (2,4-DCP) could effectively enhance PCP sorption. The PCP sorption coefficient increases in the order: PCP/phenol < PCP/2,4-DCP < PCP/phenol/2,4-DCP. The PCP sorption enhancement is likely resulted from the interlayer space expansion and the organic carbon content increase. On the basis of the comparison between the single and the multiple solute systems, phenol and 2,4-DCP sorption coefficients decrease in the order: Kd of a single solute system > Kd of a binary solute system > Kd of a ternary solute system, and no enhancement for phenol and 2,4-DCP sorption was observed.
    Journal of Chemical & Engineering Data 08/2013; 58(9):2610–2615. DOI:10.1021/je400505j · 2.05 Impact Factor
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    ABSTRACT: Understanding and controlling the process of electrochemical deposition (ECD) of a mineralized collagen coating on metallic orthopedic implants is important for engineering highly bioactive coatings. In this work, the influence of different ECD parameters was investigated. The results showed that the mineralization degree of the coatings increased with deposition time, voltage potential and H2O2 addition, while chitosan addition led to weakening of mineralization, heavy mineralization resulted in a porous coating morphology. Furthermore, two typical coatings, dense and porous, were analyzed to investigate their microstructure and evaluated for their cytocompatibility; the dense coating showed better osteoblast adhesion and proliferation. Based on our understanding of how the different coating parameters influenced the coating, we proposed an ECD process in which the pH gradient near the cathode and the collagen isoelectric point were suggested to play crucial roles in controlling the mineralization and morphology of the coatings. The proposed ECD process may offer a guide for controlled deposition of a desired bioactive coating.
    Journal of Materials Science Materials in Medicine 08/2013; 24(12). DOI:10.1007/s10856-013-5028-9 · 2.38 Impact Factor
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    ABSTRACT: To investigate the expression of suppressor of cytokine signaling(SOCS)-3 and caspase-3 and their correlative significance in endometriosis. Immunohistochemical EnVision method was used to detect the SOCS-3 and caspase-3 protein expression in ectopic and eutopic endometrium (n = 32) of patients with endometriosis, as well as normal endometrium (n = 30) of women without endometriosis. SOCS-3 and caspase-3 proteins were expressed in all three groups and not affected by the menstrual cycles. The expression of SOCS-3 in ectopic endometrium (5.54 ± 2.12) was significantly lower than that in eutopic (7.39 ± 1.09, P = 0.001) and control group (7.48 ± 1.26, P < 0.01), but without difference between the eutopic and control group (P = 0.756). SOCS-3 expression in ectopic and eutopic endometrium was significantly lower in III/IV stages than that in I/II stages of endometriosis (P < 0.05). Significantly lower expression of caspase-3 protein was found in ectopic (3.20 ± 1.24) and eutopic endometrium (3.88 ± 1.93) as compared with the control group (6.49 ± 1.85, P < 0.01), however ectopic and eutopic endometrium showed no significant difference (t = 1.66, P = 0.10). There was no significant difference of the expression of caspase-3 in ectopic and eutopic endometrium at different disease stages (P > 0.05). Positive correlation was found between the expression of SOCS-3 and caspase-3 proteins in ectopic endometrium (r = 0.655, P < 0.01). SOCS-3 may be involved in the development of endometriosis through inhibition of apoptosis of ectopic endometrial cells.
    Zhonghua bing li xue za zhi Chinese journal of pathology 08/2013; 42(8):515-8.

Publication Stats

391 Citations
155.74 Total Impact Points


  • 2003–2015
    • Zhejiang University
      • • First Affiliated Hospital of Medical College
      • • School of Medicine
      • • Department of Stomatology
      Hang-hsien, Zhejiang Sheng, China
  • 2012–2014
    • Christian-Albrechts-Universität zu Kiel
      Kiel, Schleswig-Holstein, Germany
  • 2005–2014
    • Zhejiang Medical University
      • Department of Gynecology
      Hang-hsien, Zhejiang Sheng, China
  • 2005–2009
    • Women & Infants Hospital
      Providence, Rhode Island, United States