Xing-Quan Zhu

Lanzhou Veterinary Research Institute, Kao-lan-hsien, Gansu Sheng, China

Are you Xing-Quan Zhu?

Claim your profile

Publications (242)758.15 Total impact

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Porcine parvovirus (PPV) is the important causative agent for infectious infertility, which is a fairly tough virus that multiplies normally in the intestine of pigs without causing clinical signs in the world. We developed an assay integrating real-time PCR and high resolution melting (HRM) analysis for the detection of PPV. Primers targeting the VP gene were highly specific, as evidenced by the negative amplification of closely related viruses, such as porcine circovirus 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), classical swine fever virus (CSFV), or Japanese encephalitis virus (JEV). The performance of unlabeled real time PCR was compared to TaqMan real time PCR, and the detection limits of the two methods were nearly equal. Moreover, there was good correlation between Cp and diluted genomic DNA when tested with the two methods. The assay has the accuracy of 100% in reference to labeled real time PCR, when it was tested on 45 clinical samples. The present study demonstrated that the established assay integrating real-time PCR and HRM is relatively cost-effective and more stable, which provides an alternative tool for rapid, simple, specific and sensitive detection of PPV.
    BMC Veterinary Research 12/2015; 11(1):364. DOI:10.1186/s12917-015-0364-2 · 1.74 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Gongylonema pulchrum (Nematoda: Gongylonematidae), a thread-like spirurid gullet worm, infects a range of mammalian definitive hosts, including cattle, pigs, equines, goats, primates and humans, and can cause gongylonemiasis. In the present study, the complete mitochondrial (mt) genome of G. pulchrum was obtained using Long-range PCR and subsequent primer walking. The phylogenetic position of G. pulchrum within the Spiruromorpha was established using Bayesian analyses of the protein-coding genes at the amino acid level. The length of this AT-rich (75.94%) mt genome is 13,798 bp. It contains 12 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes and one non-coding region. The gene arrangement is the same as those of Thelazia callipaeda (Thelaziidae) and Setaria digitata (Onchocercidae), but distinct from that of Heliconema longissimum (Physalopteridae). Phylogenetic analyses, based on the concatenated amino acid sequence data for all 12 protein-coding genes using Bayesian inference (BI) method, showed that G. pulchrum (Gongylonematidae) was more closely related to Spirocerca lupi (Spiruroidea) than other members of the infraorder Spiruromorpha. The present study represents the first mt genome sequence for the family Gongylonematidae, which provides the opportunity to develop novel genetic markers for studies of epidemiology, population genetics and systematics of this nematode of human and animal health significance.
    Parasites & Vectors 12/2015; 8(1). DOI:10.1186/s13071-015-0697-5 · 3.25 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Toxoplasma gondii, a protozoan parasite, infects almost all warm-blooded animals and humans. Limited information is available about T. gondii infection in Tibetan Sheep in Gansu province, northwestern China. In the present study, we estimated the seroprevalence and risk factors of T. gondii infection in this region of China. A total of 1732 Tibetan Sheep were included from Tianzhu and Maqu in Gansu province. Antibodies to T. gondii were examined by modified agglutination test (MAT), and 352 (20.3%) out of 1732 Tibetan sheep were found positive. Multivariate logistic regression analysis was used to analyze the risk factors associated with seroprevalence, the results showed that age, gender, and numbers of past pregnancies were not the significant risk factors. However, Tibetan sheep in Maqu had a 1.64 times (odds ratio [OR] =1.637, 95% CI =1.291-2.075, P < 0.001) higher seroprevalence compared to Tianzhu, and the seropositivity in summer were 1.61 times (OR =1.608, 95% CI =1.122-2.303, P = 0.010) higher compared to Tibetan sheep in winter, followed by 1.42 times (OR =1.419, 95% CI =1.002-2.011, P = 0.049) in spring. Thus, season and location were considered as risk factors associated with T. gondii infection in this study. This is the first report of T. gondii seroprevalence in Tibetan sheep in Gansu province, which enriches the epidemiological data of T. gondii infection in Tibetan sheep in China. The results of this study indicate that Tibetan sheep in Gansu province are frequently exposed to T. gondii, posing a direct threat to the public health as well as to local sheep industry. These data is useful to strengthen future prevention and control of T. gondii infection in Tibetan sheep in this region.
    BMC Veterinary Research 12/2015; 11(1). DOI:10.1186/s12917-015-0358-0 · 1.74 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: In the present study, near-complete mitochondrial (mt) genome sequences for Schistosoma japonicum from different regions in the Philippines and Japan were amplified and sequenced. Comparisons among S. japonicum from the Philippines, Japan, and China revealed a geographically based length difference in mt genomes, but the mt genomic organization and gene arrangement were the same. Sequence differences among samples from the Philippines and all samples from the three endemic areas were 0.57-2.12 and 0.76-3.85 %, respectively. The most variable part of the mt genome was the non-coding region. In the coding portion of the genome, protein-coding genes varied more than rRNA genes and tRNAs. The near-complete mt genome sequences for Philippine specimens were identical in length (14,091 bp) which was 4 bp longer than those of S. japonicum samples from Japan and China. This indel provides a unique genetic marker for S. japonicum samples from the Philippines. Phylogenetic analyses based on the concatenated amino acids of 12 protein-coding genes showed that samples of S. japonicum clustered according to their geographical origins. The identified mitochondrial indel marker will be useful for tracing the source of S. japonicum infection in humans and animals in Southeast Asia.
    Parasitology Research 04/2015; DOI:10.1007/s00436-015-4475-2 · 2.33 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Toxoplasma gondii, an obligate intracellular parasite, is able to infect many animal species and humans, and can cause toxoplasmosis of the host. In this study, we examined sequence variation in rhoptry protein 47 (ROP47) gene among T. gondii isolates originating from different hosts and geographical regions. The entire genome region of the ROP47 gene was amplified and sequenced, and phylogenetic relationship was reconstructed using maximum parsimony (MP), maximum likelihood (ML) and neighbor-joining (NJ), based on the ROP47 gene sequences. The results of sequence alignments showed that all ROP47 gene sequences were 396 bp in length. There were 19 variable nucleotide positions in the coding region, resulted in 16 amino acid substitutions (12.21%) among all examined T. gondii strains and the existence of polymorphic restriction sites for endonucleases SacI and AflIII, allowing the differentiation of the three major clonal lineage types I, II and III by PCR-RFLP. Phylogenetic analysis of ROP47 gene sequences showed that three major clonal lineage types I, II and III were clustered differently, consistent with PCR-RFLP results. These results suggest that ROP47 gene sequence may represent a potential novel genetic marker for population genetic studies of T. gondii isolates. Copyright © 2015 Elsevier Inc. All rights reserved.
    Experimental Parasitology 04/2015; DOI:10.1016/j.exppara.2015.03.006 · 1.86 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Background Rumen flukes parasitize the rumen and reticulum of ruminants, causing paramphistomiasis. Over the years, there has been considerable debate as to whether Paramphistomum leydeni and Paramphistomum cervi are the same or distant species. Methods In the present study, the complete mitochondrial (mt) genome of P. leydeni was amplified using PCR-based sequencing and compared with that of P. cervi. The second internal transcribed spacer (ITS-2) of nuclear ribosomal DNA (rDNA) of P. leydeni specimens (n = 6) and P. cervi specimens (n = 8) was amplified and then sequenced. Phylogenetic relationship of the concatenated amino acid sequence data for 12 protein-coding genes of the two rumen flukes and selected members of Trematoda was evaluated using Bayesian inference (BI). Results The complete mt genome of P. leydeni was 14,050 bp in size. Significant nucleotide difference between the P. leydeni mt genome and that of P. cervi (14.7%) was observed. For genetic divergence in ITS-2, sequence difference between P. leydeni and P. cervi was 3.1%, while no sequence variation was detected within each of them. Phylogenetic analysis indicated that P. leydeni and P. cervi are closely-related but distinct rumen flukes. Conclusions Results of the present study support the proposal that P. leydeni and P. cervi represent two distinct valid species. The mt genome sequences of P. leydeni provide plentiful resources of mitochondrial markers, which can be combined with nuclear markers, for further comparative studies of the biology of P. leydeni and its congeners from China and other countries. Electronic supplementary material The online version of this article (doi:10.1186/s13071-015-0823-4) contains supplementary material, which is available to authorized users.
    Parasites & Vectors 04/2015; 8. DOI:10.1186/s13071-015-0823-4 · 3.25 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Schistosomiasis has decreased significantly in prevalence and intensity of infection in China, thus more accurate and sensitive methods are desperately needed for the further control of schistosomiasis. The present work aimed to assess the utility of the loop-mediated isothermal amplification (LAMP) for detection of light intensity infection or false-negative patients and patients post-treatment, targeting the highly repetitive retrotransposon SjR2 of Schistosoma japonicum. LAMP was first assessed in rabbits with low intensity infection (EPG<10). Then 110 patient sera from Hunan Province, China, and 47 sera after treatment by praziquantel were used to evaluate the diagnostic validity of LAMP. Meanwhile, 42 sera from healthy individuals in a non-endemic area, and 60 sera from "healthy" residents who were identified as being negative for feces examination and immuno-methods in an endemic area were also examined. The results showed that LAMP could detect S. japonicum DNA in sera from rabbits at 3rd day post-infection. Following administration of praziquantel, the S. japonicum DNA in rabbit sera became negative at 10 weeks post-treatment. Of 110 sera from patients, LAMP showed 95.5% sensitivity, and even for 41 patients with less than 10 EPG, the sensitivity of LAMP still reached to 95.1%. For 47 patients after treatment, the negative conversion rate of S. japonicum DNA in patient sera increased from 23.4%, 61.7% to 83.0% at 3 months, 6 months and 9 months post-treatment, respectively. No false-positive result was obtained for 42 human sera from non-endemic area, while for the 60 "healthy" individuals from endemic area, 10 (16.7%) individuals were positive by LAMP, which suggested that these individuals might be false-negative patients. The present study demonstrated that the LAMP assay is sensitive, specific, and affordable, which would help reduce schistosomiasis transmission through targeted treatment of individuals, particularly for those with negative stool examinations who may yet remain infected. The LAMP assay may provide a potential tool to support schistosomiasis control and elimination strategies.
    PLoS Neglected Tropical Diseases 04/2015; 9(4):e0003668. DOI:10.1371/journal.pntd.0003668 · 4.49 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: To evaluate the protective efficacy of a DNA vaccine encoding Toxoplasma gondii rhoptry protein 5 (ROP5) and GRA15 antigens. We constructed eukaryotic plasmids expressing pVAX-ROP5 and pVAX-GRA15, and measured the immune responses to these DNA vaccines. Kunming mice immunized with pVAX-ROP5 or pVAX-GRA15 showed significantly increased serum IgG2a titers; Th1 responses association with the production of IFN-γ, IL-2, IL12 p40 and IL-12 p70; cell-mediated cytotoxic activity with increased frequencies of IFN-γ secreting CD8(+) T cells (CD8(+) IFN-γ+ T cells), as well as prolonged survival time (19.4 ± 4.9 days for ROP5; 17.8 ± 3.8 days for GRA15) and brain cyst reduction (57.4% for ROP5; 65.9% for GRA15) compared to control mice. Co-administration with pVAX-ROP5 and pVAX-GRA15 boosted the cellular and humoral immune responses, and significantly increased cyst reduction (79%) and prolonged the survival of immunized mice (22.7 ± 7.2 days). Co-immunization of pVAX-ROP5 and pVAX-GRA15 increase the protective efficacy.
    Expert Review of Vaccines 04/2015; 14(4):617-24. DOI:10.1586/14760584.2015.1011133 · 4.22 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: White yaks, a unique yak breed and the pearl of the plateau, only live in Tianzhu Tibetan Autonomous County (TTAC), Gansu Province, northwest China, contributing significantly to local economy. However, there was no information on the prevalence of Babesia bigemina in white yaks. In this study, a total of 974 serum samples collected from white yaks in TTAC were examined for specific antibodies against Babesia bigemina using a commercially available ELISA kit. The overall seroprevalence of B. bigemina in white yaks was 17.76% (173/974). A multivariate logistic regression analysis was performed to determine the risk factors associated with B. bigemina seroprevalence, and the results indicated that age, gender and the numbers of pregnancies of white yaks were not the significant risk factors. However, the white yaks in spring (OR=3.523, 95% CI=1.899-6.538, P < 0.001) and summer (OR=3.439, 95% CI=1.909-6.193, P < 0.001) encountered higher risk of being exposed to B. bigemina than that in winter. Thus, season was considered as a risk factor associated with B. bigemina infection. This is the first survey of B. bigemina seroprevalence in white yaks in China, which extends the host range for B. bigemina and provides useful information for controlling B. bigemina infection in white yaks. Copyright © 2015. Published by Elsevier B.V.
    Acta Tropica 02/2015; 145. DOI:10.1016/j.actatropica.2015.02.005 · 2.52 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Toxocara canis is a zoonotic parasite of major socioeconomic importance worldwide. In humans, this nematode causes disease (toxocariasis) mainly in the under-privileged communities in developed and developing countries. Although relatively well studied from clinical and epidemiological perspectives, to date, there has been no global investigation of the molecular biology of this parasite. Here we use next-generation sequencing to produce a draft genome and transcriptome of T. canis to support future biological and biotechnological investigations. This genome is 317 Mb in size, has a repeat content of 13.5% and encodes at least 18,596 protein-coding genes. We study transcription in a larval, as well as adult female and male stages, characterize the parasite's gene-silencing machinery, explore molecules involved in development or host-parasite interactions and predict intervention targets. The draft genome of T. canis should provide a useful resource for future molecular studies of this and other, related parasites.
    Nature Communications 02/2015; 6:6145. DOI:10.1038/ncomms7145 · 10.74 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Prevalence of human infection with Toxoplasma gondii has been increasing in China due to increasing number of cats. However, little is known of the epidemiology of T. gondii infection in different cancer patient groups. Thus, a case-control study of 900 cancer patients and 900 controls was conducted to detect anti-T. gonii antibodies by ELISA in China. Genomic DNA was extracted from the diseased tissues of 510 patients and T. gondii B1 gene was amplified using a semi-nested PCR. DNA samples giving positive B1 amplification were then genetically characterized using multi-locus PCR-RFLP. The prevalence of anti-T. gonii IgG in cancer patients (35.56%) was significantly higher than that in controls (17.44%). The highest T. gondii seroprevalence was detected in lung cancer patients (60.94%), followed by cervical cancer patients (50%), brain cancer patients (42.31%) and endometrial cancer patients (41.67%). Exposure with soil and consumption of raw/undercooked meat were significantly associated with T. gondii infection in cancer patients. Three T. gondii genotypes (ToxoDB#9, ToxoDB#10 and Type I variant) were identified. In conclusion, T. gondii infection is a severe problem in cancer patients and it is imperative that improved integrated measures should be conducted to prevent and control T. gondii infection in cancer patients. Copyright © 2015. Published by Elsevier Ireland Ltd.
    Cancer Letters 01/2015; 359(2). DOI:10.1016/j.canlet.2015.01.036 · 5.02 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Background Toxoplasma gondii is a protozoan parasite that infects almost all warm-blooded animals and human beings. Goats are one of the susceptible animals to T. gondii. However, little is known of genetic diversity of T. gondii in Yunnan black goats in China. The objective of this present study was to determine the genotypes of T. gondii isolates from black goats in Yunnan province, southwest China.Methods Genomic DNA was extracted from liver (n¿=¿403), lung (n¿=¿403) and lymph nodes (n¿=¿250) of Yunnan black goats and assayed for T. gondii infection by semi-nested PCR of B1 gene. Then, the positive DNA samples were typed at 10 genetic markers using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology. These markers include 9 nuclear loci, namely, SAG1, SAG2 (5¿-SAG2 and 3¿-SAG2, alternative SAG2), SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and an apicoplast locus Apico.ResultsOut of 403 tested samples, 20 (4.96%) DNA samples were T. gondii positive by amplification of B1 gene. Among them, 2 isolates were genotyped at all loci, and 6 isolates were genotyped for 8 or more loci. In total, seven samples belong to ToxoDB PCR-RFLP genotype#10 (Type I), and one belongs to genotype ToxoDB #9.Conclusions To our knowledge, this is the first report of ToxoDB#9 and ToxoDB#10¿T. gondii in Yunnan black goats in China. These results revealed a wide distribution of these T. gondii in Yunnan black goats in China, which has important implications for public health.
    Parasites & Vectors 01/2015; 8(1):57. DOI:10.1186/s13071-015-0673-0 · 3.25 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Background Chlamydia is gram-negative obligate bacteria which causes a wide variety of diseases in humans and animals. To date, there are a few reports about the seroprevalence of Chlamydia and the risk factors associated with Chlamydia infection in yaks in the world. In this study, 974 blood samples were collected from white yaks (Bos grunniens) in Tianzhu Tibetan Autonomous County, Gansu province, northwest China from June 2013 to April 2014.ResultsAntibodies against Chlamydia abortus were examined by the indirect hemagglutination (IHA) test, and 158 of 974 (16.22%) white yaks were seropositive for C. abortus antibodies at the cut-off of 1:16. The risk factors associated with seroprevalence were evaluated by a multivariate logistic regression analysis. Region, gender and age of white yak were left out of the final model, due to its insignificance in the logistic regression analysis (P¿>¿0.05). However, season was considered as a major risk factor associated with C. abortus infection in white yaks.Conclusions To our knowledge, this is the first survey of C. abortus seroprevalence in white yaks in China, which extends the host range for C. abortus and has important implications for public health and the local Tibetan economy.
    BMC Veterinary Research 01/2015; 11(1):8. DOI:10.1186/s12917-015-0323-y · 1.74 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Granulomatous and fibrosing inflammation in response to parasite eggs is the main pathology that occurs during infection with Schistosoma spp. CD4+ T cells play critical roles in both host immune responses against parasitic infection and immunopathology in schistosomiasis,and coordinate many types of immune cells that contribute to fibrosis. ICOSL plays an important role in controlling specific aspects of T cell activation, differentiation, and function. Previous work has suggested that ICOS is essential for Th17 cell development. However, the immunopathogenesis of this pathway in schistosomiasis fibrosisis still unclear. Using models of schistosomiasis in ICOSL KO and the C57BL/6 WT mice, we studied the role of the ICOSL/ICOS interaction in the mediation of the Th17 response in host granulomatous inflammation, particularly in liver fibrosis during S. japonicum infection, and investigated the immune responses and pathology of ICOSL KO mice in these models. The results showed that ICOSL KO mice exhibited improved survival, reduced liver granulomatous inflammation around parasite eggs, markedly inhibited hepatic fibrosis development, lower levels of Th17-related cytokines (IL-17/IL-21), Th2-related cytokines (IL-4/IL-6/IL-10), a pro-fibrotic cytokine (IL-13), and TGF-β1, but higher level of Th1-related cytokine (IFN-γ) compared to wild-type (WT) mice. The reduced progression of fibrogenesis was correlated with the down-regulation of Th17 and Th2 and the elimination of ICOSL/ICOS interactions. Our findings suggest that IL-17-producing cells contribute to the hepatic granulomatous inflammation and subsequent fibrosis. Importantly, there was a clearly positive correlation between the presence of IL-17-producing cells and ICOS expression in ICOSL KO mice, and additional results indicated that Th17 was involved in the pathological tissue remodeling in liver fibrosis induced by schistosomiasis.
    PLoS Neglected Tropical Diseases 01/2015; 9(1):e0003434. DOI:10.1371/journal.pntd.0003434 · 4.49 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Background Toxoplasmosis caused by the intracellular parasite Toxoplasma gondii (T. gondii) is a global epidemic parasitic disease. DNA vaccines play an important role in preventing the spread of toxoplasmosis. SAG family genes encoding particular surface proteins of T. gondii are the best candidates of DNA vaccine. As a member of SAG family genes, SAG5 gene has been proved to have better antigenic than SAG1. In addition, alpha-Galactosylceramide (¿-GalCer) was used to be an adjuvant in malaria vaccine and received positive results. In this study, the effect of the DNA vaccine enhanced by ¿-GalCer was evaluated by immunizing BALB/c mice.Methods In the present study, SAG5D gene of T. gondii was cloned, sequenced, and biologically characterized. BALB/c mice were randomly divided into five groups, including three experimental groups (pEGFP-C1-SAG5D, ¿-GalCer and ¿-GalCer/pEGFP-C1-SAG5D) and two control groups (PBS and pEGFP-C1), and were immunized intramuscularly three times. The levels of IgG antibodies and cytokine productions in mouse sera were determined by enzyme-linked immunosorbent assays (ELISA). Two weeks after the last immunization, all mice were challenged intraperitoneally with 1¿×¿104 tachyzoites of T. gondii and the survival time of mice was recorded.ResultsA significant level of increase of IgG response against the soluble tachyzoite antigens (STAg) was detected by ELISA in experimental group. It revealed relatively high level of IFN-¿ production by the spleen cells. There were higher productions of interleukin-4 (IL-4) in ¿-GalCer treated groups compared to control groups. Challenge experiment showed a longer survival period (11 days compared with 5 days in control) in SAG5D DNA vaccinated mice was found after a lethal challenge with T. gondii RH strain.Conclusions The present study suggested that T. gondii SAG5D was a novel and positive DNA vaccine candidate against toxoplasmosis. In addition, the adjuvant (¿-GalCer) enhanced the body¿s cellular immune response and prolonged the survival time of mice after challenge.
    BMC Infectious Diseases 12/2014; 14(1):706. DOI:10.1186/s12879-014-0706-x · 2.56 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Background Toxoplasma gondii is an intracellular protozoan parasite that infects a wide variety of warm-blooded hosts, including humans. Limited information about T. gondii infection in bats is available in China. The objective of the present study was to determine prevalence and genetic characterization of T. gondii infection in bats in Jilin, Liaoning, Jiangxi and Guangdong provinces, China.Methods During May 2005 to August 2013, bats were sampled from Jilin, Liaoning, Jiangxi, and Guangdong provinces, China, and liver tissues were collected for the detection of T. gondii by a nested PCR targeting the B1 gene. The positive samples were genotyped at 11 genetic markers (SAG1, 5¿-and 3¿-SAG2, alternative SAG2, SAG3, BTUB, GRA6, L358, PK1, c22-8, c29-2, and Apico) using multilocus polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).ResultsA total of 626 bats representing 10 species were examined for T. gondii infection, 38 (6.1%) were tested positive with by PCR, 8 positive DNA samples were completely genotyped, of which 3 samples (2 from Cynopterus sphinx, and 1 from Murina leucogaster) represented ToxoDB#10, and 5 samples (2 from Murina leucogaster, 2 from Myotis chinensis, and 1 from Rhinolophus ferrumequinum) belonged to ToxoDB#9 (http://toxodb.org/toxo/).Conclusions The present study revealed an overall T. gondii prevalence of 6.1% in bats from Jilin, Liaoning, Jiangxi and Guangdong provinces in China, and reported two T. gondii genotypes (ToxoDB#9 and #10) having a wide geographical distribution in China. These results provide new genetic information about T. gondii infection in bats, and have implications for better understanding of the genetic diversity of T. gondii in China and elsewhere.
    Parasites & Vectors 12/2014; 7(1):558. DOI:10.1186/PREACCEPT-1950109686141001 · 3.25 Impact Factor
  • 10/2014; 39(5):539-542. DOI:10.3724/SP.J.1238.2013.00539
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Background: Toxoplasma gondi(is an obligate intracellular parasite which can infect almost all mammalian animals leading to toxoplasmosis. I goods rhoptiy protein 38 (TgROP38) is an active rhoptiy protein kinase which is involved in the inhibitory effect on host cell transcription by down regulating the MAPK signaling track. Methods: TgROP38 gene was amplified and inserted into eukaryotic vector pVAX I and formed the DNA vaccine pVAX-ROP38. Mice in the experimental group were intramuscularly immunized with pVAX-ROP38 and those injected with pVAX I, PBS or nothing were treated as controls. After three injections at two week intervals, all mouse groups were challenged intraperitoneally with 1000 tachyzoites of the virulent T gondii RH strain (Type I, ToxoDB #10) and 10 cysts of the FRU strain (Type II, ToxoDB it1), respectively. Results: Mice inoculated with pVAX-ROP38 vaccine had a higher level of IgG antibodies (P < 0.011 arid T lymphoproliferative response. The high ratio of IgG2a/IgG1 and the increasing levels of IFN y and IL 2 (P <005) indicated an activated Thl cell mediated immune responses. Furthermore, the CD4 and CD8' proportions in vaccinated mice were also increased significantly compared with that in mice of the three control groups (P <001). In the model of acute infection, the average survival time of mice in the pVAX-ROP38 group (8.1 days 0.75) was no statistically different compared to that in the PBS, pVAX I and blank control groups which died within 7 days. However, in the model of chronic infection, the brain cyst reduction in the pVAX-ROP38 group reached 76.6%, compared to controls (P< 0.01). Conclusions: The present study revealed that the pVAX-ROP38 vaccine could elicit strong humoral and cell immunity response against chronic T gondii infection in mice, resulting in the reduction of the brain cyst formation effectively, which suggests that TgROP38 is a desirable vaccine candidate against chronic T gondii infection.
    BMC Infectious Diseases 09/2014; 14(1):525. DOI:10.1186/1471-2334-14-525 · 2.56 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Hepatitis E, caused by the hepatitis E virus (HEV), is an important global public health concern. There are conflicting reports in regard to the association of HEV infection and psychiatric disorders. Therefore, we investigated this association in a Chinese population through a case-control seroprevalence study.
    International Journal of Infectious Diseases 09/2014; DOI:10.1016/j.ijid.2014.07.023 · 2.33 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Toxoplasma gondii can infect all warm-blooded animals including humans. Infection with T. gondii is probably the leading cause of posterior uveitis in humans and the most comment route of transmission is raw and undercooked meat from infected animals. T. gondii calcium-dependent protein kinase 1 (TgCDPK1) plays a critical role in direct parasite motility, host-cell invasion, and egress.
    BMC Infectious Diseases 09/2014; 14(1):487. DOI:10.1186/1471-2334-14-487 · 2.56 Impact Factor

Publication Stats

3k Citations
758.15 Total Impact Points

Institutions

  • 2010–2015
    • Lanzhou Veterinary Research Institute
      Kao-lan-hsien, Gansu Sheng, China
    • University of Jinan (Jinan, China)
      Chi-nan-shih, Shandong Sheng, China
  • 2014
    • Academy of Military Medical Sciences
      T’ien-ching-shih, Tianjin Shi, China
  • 2013–2014
    • Jilin Agricultural University
      Yung-chi, Jilin Sheng, China
  • 2011–2014
    • Heilongjiang Bayi Agricultural University
      Sa-erh-t’u, Heilongjiang Sheng, China
    • Hunan Agricultural University
      • College of Veterinary Medicine
      Ch’ang-sha-chen, Jiangsu Sheng, China
  • 2007–2014
    • Yunnan Agricultural University
      Panlong, Shaanxi, China
    • Sun Yat-Sen University
      • Animal Experiment Center
      Guangzhou, Guangdong Sheng, China
  • 2012
    • National Institute of Parasitic Diseases
      Shanghai, Shanghai Shi, China
  • 2010–2011
    • Chinese Academy of Agricultural Sciences
      Peping, Beijing, China
  • 2008–2010
    • South China Agricultural University
      • College of Veterinary Medicine
      China
  • 2005
    • China Agricultural University
      Peping, Beijing, China