[show abstract][hide abstract] ABSTRACT: In this study we obtained Fourier transform infrared (FTIR) spectra of fixed prostate cell lines of differing types as well as the primary epithelial cells from benign prostatic hyperplasia (BPH). Results showed that by using multivariate chemometric analysis it was possible to discriminate and classify these cell lines, which gave rise to sensitivity and specificity values of >94% and >98%, respectively. Following on from these results the possible influences of different factors on the discrimination and classification of the prostate cell lines were examined. Firstly, the effect of using different growth media during cell culturing was investigated, with results indicating that this did not influence chemometric discrimination. Secondly, differences in the nucleus-to-cytoplasm (N/C) ratio were examined, and it was concluded that this factor was not the main reason for the discrimination and classification of the prostate cancer (CaP) cell lines. In conclusion, given the fact that neither growth media nor N/C ratio could totally explain the classification it is likely that actual biochemical differences between the cell lines is the major contributing factor.
The Analyst 07/2009; 134(6):1083-91. · 4.23 Impact Factor
[show abstract][hide abstract] ABSTRACT: Recent epidemiological studies have positively associated total dietary fat intake to prostate cancer (CaP) incidence and progression. However, the role of fat-specific intake remains unclear through these patient-orientated studies, which in-turn warrants the need for controlled molecular based investigations. FTIR microspectroscopy is now a well established tool for the metabolomic analysis of a wide variety of biomolecules at the whole cell level and can be used to generate hypotheses to refine molecular specific experiments. For the first time, we have used FTIR microspectroscopy to measure the uptake and metabolism of the saturated fatty acid (FA), palmitic acid (PA) and the unsaturated FA, arachidonic acid (AA) using deuterated analogues. We also report on the temporal fluctuations of different biomolecular domains (lipid, phosphate and protein secondary structure) within PC-3 cells in response to D8-AA and D31-PA uptake. Our results demonstrate that (i) FTIR can be used to track eicosonoid generation in D8-AA stimulated cells; (ii) the intracellular management of D31-PA at high-loadings can be elucidated by monitoring lipid biosynthesis through the lipid hydrocarbon peak area signal; (iii) metabolism of lipid pools results in significant protein phosphorylation.
[show abstract][hide abstract] ABSTRACT: Here we report on investigations into using Raman optical tweezers to analyse both live and chemically fixed prostate and bladder cells. Spectra were subjected to chemometric analysis to discriminate and classify the cell types based on their spectra. Subsequent results revealed the potential of Raman tweezers as a potential clinical diagnostic tool.