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ABSTRACT: BACKGROUND: Landmine injuries cause extensive soft and bony tissue loss of the weight-bearing areas, particularly the heel. Reconstruction of these injuries is challenging, and there are no studies that report long-term functional results. OBJECTIVE: To determine the quality of life and long-term functionality of patients who had heel reconstruction with free muscle flap after landmine injuries. DESIGN: A case-control study. PARTICIPANTS: Nine male patients who had heel reconstruction with free muscle flap. Ten male volunteers without any gait disorder were included in the study as the control group. METHODS AND INTERVENTIONS: Functional ambulation scale, visual analog scale, energy expenditure index, 6-minute walking test, 10-m walking test, and Short Form 36 were performed to determine the quality of life and functionality of the participants. RESULTS: There were no statistically significant differences between the 2 groups in terms of functional ambulation scale, energy expenditure index, 6-minute walking test, and 10-m walking test. Regarding Short Form 36 scores, all subgroup values were lower in the reconstruction group, whereas only those of general health, vitality, and physical-emotional role limitation subgroups showed statistical significance. Mean visual analog scale scores were found to be statistically different between the groups (P < .05). Mean Freiburg Ankle scores showed moderate functionality. CONCLUSION: Despite the associated physical and emotional trauma, combat-injured veterans with heel reconstruction after landmine injuries had adequate and functional ambulation at long-term follow-up. Early rehabilitation and close cooperation between surgeons and rehabilitation physicians during the care of these patients will enhance patient outcomes.
Der Notarzt 02/2013; · 0.28 Impact Factor
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ABSTRACT: Cellular and vascularized bone marrow cells have been used to induce donor-specific chimerism in various models of composite tissue allotransplantation. Although thymus transplantation has been reported in the literature, the effect of thymus transplantation on chimerism levels in vascularized bone containing composite tissue allotransplantation has not been reported. In this study, a new method for composite vascularized sternal bone marrow transplant model is descried that can be applied to augment chimerism after transplantation. A total of seven composite osseomusculocutaneous sternum, ribs, thymus, pectoralis muscles, and skin transplantations were performed in two groups. The first group (n = 5) was designed as an allotransplantation group and the second group (n = 2) was designed as an isotransplantation group. Composite osseomusculocutaneous sternum, ribs, thymus, and pectoralis muscles allografts were harvested on thecommon carotid artery and external jugular vein and a heterotopic transplantation was performed to the inguinal region of the recipient rat. Cyclosporine A monotherapy was administered in order to prevent acute and chronic allograft rejection. Animals sacrificed when any sign of rejection occurred. The longest survival was 156 day post-transplant. Assessment of bone marrow cells within sternum bone component and flow cytometry analysis of donor-specific chimerism in the peripheral blood of recipients were evaluated. Our results showed that thiscomposite allograft carried 7.5 × 10(6) of viable hematopoietic cells within the sternum component. At day 7 post-transplant chimerism was developed in T-cell population and mean level was assessed at 2.65% for RT1(n) /CD4 and at 1.0% for RT1(n) /CD8. In this study, a newosseomusculocutaneous sternum, ribs, thymus, pectoralis muscle, and skin allotransplantation model is reported which can be usedto augment hematopoietic activity for chimerism induction after transplantation. © 2012 Wiley Periodicals, Inc. Microsurgery, 2012.
Microsurgery 07/2012; · 1.61 Impact Factor
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ABSTRACT: The use of the topical tissue adhesive N-butyl-2-cyanoacrylate (Indermil™, Tyco Healthcare Group LP, Norwalk, CT, USA) in a 22-year-old male with neurofibromatosis
type 1 who was admitted to the emergency room because of uncontrollable bleeding is presented. The topical tissue adhesive
was applied to the bleeding from the neurofibromatous tissue. Oozing from the tissue stopped within seconds. Vascular abnormalities
associated with neurofibromatosis type 1 must be kept in mind during excision of neurofibroma from the head and neck region.
Tissue adhesive may be used in emergency situations such as the case presented here.
European Journal of Plastic Surgery 04/2012; 31(4):193-195.
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ABSTRACT: Corrugator resection is an integral part of periorbital rejuvenation and can be accomplished through the open coronal, endoscopic, or transpalpebral technique. While most authors concur about the importance of corrugator resection during brow lift surgery, considerable debate remains regarding the efficacy and technical ease of muscle resection with these approaches.
The authors conducted a cadaver study to compare the completeness of resection of the corrugator muscle with the transpalpebral and endoscopic techniques.
A split-face study was performed in 10 fresh cadavers. On one side, the corrugator muscle was resected through an endoscopic approach, and on the opposite side of the face, a transpalpebral resection was performed. After the completion of both procedures, a coronal incision was made; gross observations were noted; photographs were taken; and muscle weights were recorded.
In 19 of 20 cadaver halves, subtotal or total resection of the corrugator muscle was accomplished. In only one endoscopic instance was any muscle left in continuity. This occurred along the superior aspect of the arcus marginalis release when the corrugator muscle was hidden by the upper edge of the cut periosteum.
Unlike the previous reports, the authors found that transpalpebral and endoscopic techniques both allow subtotal or total resection of the corrugator muscle. Inadequate resection is technique dependent rather than procedure dependent.
Aesthetic surgery journal / the American Society for Aesthetic Plastic surgery 02/2012; 32(2):151-6.
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ABSTRACT: Face and hand composite tissue allotransplantations have evolved into a promising subset of reconstructive transplant surgery due to recent advances in immunotherapy. Concomitant composite tissue allotransplantation, which involves a variable combination of facial (myocutaneous versus osteomyocutaneous) and upper extremity (ie, various levels) composite subtypes, has been performed infrequently at this time. In this review, we will describe many reasons as to why this field remains unexplored. Undoubtedly, future investigation is warranted to investigate the potential advantages and disadvantages of using this approach versus a staged manner for alloreconstruction and to identify the complexities of cortical reorganization and rehabilitation in this setting.
Annals of plastic surgery 09/2011; 67(3):309-14. · 1.29 Impact Factor
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ABSTRACT: The mini- and micro-grafting method is still one of the most current treatment methods for male pattern baldness and female androgenic alopecia. The preparation of the recipient area with 16G needle has been reported in the literature. However, during the insertion of grafts, the neighboring grafts tend to `pop out'. The study presents our experience in the hair implantation for recipient site preparation with 16G epidural needle.
The 16G epidural needle was used during preparation of the recipient field in eight patients. Approximately 500 micrografts were grafted in each patient to reconstruct the anterior hairline.
During insertion of the micrografts, almost none of the previously inserted grafts tended to `pop out'. The anterior hairline of the patients was natural without obvious scarring. The mean follow-up period of these patients was 1.5 years. The amount of graft survival was found to be satisfactory.
According to the surgeon's clinical observations, application of this technique was found to be easier than standard needle techniques. It can be used by inexperienced surgeons. Use of epidural needle for recipient hole preparation works well in reducing the popping out, and the curved fashion of the holes reduced the angle of the hair follicle.
International journal of dermatology 06/2011; 50(6):736-9. · 1.18 Impact Factor
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ABSTRACT: Numerous experiments in composite tissue allotransplantation (CTA) have identified skin as the most antigenic, with recent experimentation from our laboratory finding a direct correlation between large antigenic skin loads and chimerism. Therefore, in preparation for clinical application of concomitant upper extremity (UExt) and face transplantation, we aimed to identify the exact skin quantities accompanying various upper UExt and concomitant scenarios using a cadaver study.
Five fresh cadavers were obtained and dissections were performed to simulate five different UExt transplant levels. Exact skin quantities (cm²) and total body surface area (TBSA) percentages were calculated using digital image analysis for both unilateral and bilateral hand transplant levels. UExt measurements were then cross-analyzed with our laboratory's facial/scalp alloflap data for similar evaluation of various concomitant CTA scenarios.
Skin quantities for unilateral hand transplants ranged from 335 (± 58) to 787 (± 82) cm², and from 670 (± 117) to 1575 (± 163) cm² for bilateral. Concomitant CTA quantities (from "unilateral wrist-level with face" to "bilateral elbow-level with face/scalp") extended from 1010 (± 81) to 2766 (± 202) cm², totaling a range of 5.6% to 15.4% TBSA.
The findings presented here, for the first time, define exact skin quantities and TBSA percentages accompanying unilateral, bilateral, and concomitant hand/face transplant scenarios. Unilateral UExt transplants contain between 335 and 787 cm² and bilateral between 670 and 1575 cm². Concomitant face/scalp and UExt transplants contain between 1000 and 2800 cm², equating 5% to 15% TBSA. Furthermore, there exists a tremendous void in research and some inconsistencies between animal investigation and clinical experience related to large skin-bearing CTAs. These concerns warrant further investigation by all teams pursuing concomitant CTA.
Transplantation 05/2011; 91(9):1050-6. · 4.00 Impact Factor
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ABSTRACT: When performing in situ harvesting of cranial bone grafts, there is a risk of entering the pericranial-intracranial venous system, either directly or indirectly through the arachnoid foveolae. The aims of this study were to investigate the size and location of arachnoid foveolae and to provide an anatomic road map to prevent penetrating these structures.
Three hundred dry skulls were selected from the Hamann-Todd osteological collection (Cleveland, OH); skulls were collected between 1912 and 1938. Our study skulls were limited to whites or African American adults. Exclusion criteria included children (<18 y), ethnic groups other than African Americans and whites, skulls demonstrating fracture or craniofacial abnormalities, or any skull whose age, ethnicity, and sex could not be confirmed. From the 300 skulls in the collection, 200 met the criteria and were included in our review. The mean age of these 200 individuals was 43.86 years, with a male-to-female proportion of 100:100, and a white-to-African American proportion of 144:56. A 500-W candescent light was used to transilluminate the arachnoid foveola, and digital photographs with scale were obtained. The location and diameters of foveolae for arachnoid granulations relative to the coronal and sagittal suture were measured.
Approximately 90% of major arachnoid foveolae are located within 2.5 cm of the coronal and 1.5 cm of the sagittal suture for the left and right parietal bones. Major arachnoid foveolae are located at closer distances to the superior sagittal suture and the coronal suture in the right and left parietal bone than minor foveolae. The results of this study imply that potential complications can be minimized by avoiding these areas and by harvesting in situ bone grafts from the absolute and relative safe zones described in this study.
The Journal of craniofacial surgery 01/2011; 22(1):118-21. · 0.81 Impact Factor
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ABSTRACT: Rodents, especially rats, are the most frequently used animals in composite tissue allotransplantation (CTA) studies. There
are several advantages of using rodents in experimental studies especially in transplantation studies. During the past 20
years of our research in the field of CTA transplants, we have designed and developed different craniofacial CTA models in
rats testing different immunosuppressive protocols of tolerance induction. These models include full face/scalp transplants,
hemiface transplants, composite hemiface/calvarium transplants, rat maxilla allotransplants, composite osteomusculocutaneous
hemiface/mandible/tongue-flap transplants, and composite midface allotransplants. These models and the models that will be
developed in the future will provide the scientific foundation for future success in CTA transplantation in the clinical setting.
12/2010: pages 41-53;
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ABSTRACT: From its first origination involving successful rat hind limb allograft studies using cyclosporine, face and upper extremity
transplantation (CTA) has since developed into an exciting and promising subset of reconstructive transplant surgery. Current
surgical technique involving CTA has allowed optimal outcomes in patients with massive facial and/or upper extremity defects,
however, with its coexisting immunological barrier; obligatory lifelong immunosuppression commits each patient to a daily
risk of transplant-related complications with many unanswered questions. Since 1998, nearly 50 hands in 40 patients have been
performed around the world at various levels ranging from wrist to shoulder. However, the risk-to-benefit ratio remains controversial
in bilateral versus unilateral transplantation and has yet to be answered. A total of ten face transplants have been performed
since 2005. Concomitant CTA, which involves a variable combination of allograft subtypes, has been performed in two of the
nine face transplant patients. These have included simultaneous bilateral hand transplants and tongue with mandible. Future
study is warranted to investigate the potential advantages and disadvantages of using this approach versus a staged manner
for reconstruction.
12/2010: pages 417-425;
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ABSTRACT: Peripheral nerve injury may cause gaps between the nerve stumps. Axonal proliferation in nerve conduits is limited to 10-15 mm. Most of the supportive research has been done on rat or mouse models which are different from humans. Herein we review autografts and biomaterials which are commonly used for nerve gap repair and their respective outcomes. Nerve autografting has been the first choice for repairing peripheral nerve gaps. However, it has been demonstrated experimentally that tissue engineered tubes can also permit lead to effective nerve repair over gaps longer than 4 cm repair that was previously thought to be restorable by means of nerve graft only. All of the discoveries in the nerve armamentarium are making their way into the clinic, where they are, showing great potential for improving both the extent and rate of functional recovery compared with alternative nerve guides.
Microsurgery 09/2010; 30(7):574-88. · 1.61 Impact Factor
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ABSTRACT: From its origination involving successful rat hind-limb allograft studies using cyclosporine, face and upper extremity composite tissue allotransplantation has since developed into an exciting and promising subset of reconstructive transplant surgery. Current surgical technique involving composite tissue allotransplantation has allowed optimal outcomes in patients with massive facial and/or upper extremity defects; however, with its coexisting immunologic barrier, obligatory lifelong immunosuppression commits each patient to a daily risk of transplant-related complications with many unanswered questions. Since 1998, nearly 50 hand transplantations in 40 patients have been performed around the world at various levels ranging from wrist level to shoulder level. However, the risk-to-benefit ratio remains controversial in bilateral versus unilateral transplantation and has yet to be determined. From recent experience, the two most important determinants of the success of each patient's upper extremity transplant are patient compliance and intense rehabilitation. A total of nine face transplants have been performed since 2005. Multiple aesthetic subunits (i.e., nose, lips, eyelids) with or without underlying craniofacial skeletal defects (i.e., maxilla, mandible) have been successfully restored, thereby providing restoration of vital facial functions (i.e., smiling) in an unprecedented manner. As of today, face transplantation carries an estimated 2-year mortality of 20 percent. Concomitant composite tissue allotransplantation, which involves a variable combination of allograft subtypes, has been performed in two of the nine face transplant patients. These have included simultaneous bilateral hand transplants and tongue with mandible. Future study is warranted to investigate the potential advantages and disadvantages of using this approach versus a staged approach for reconstruction.
Plastic and reconstructive surgery 07/2010; 126(1):308-15. · 2.74 Impact Factor
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ABSTRACT: Surgical treatment of calvarial defects and deformities has some drawbacks such as donor site morbidity or use of allogenic materials. In this experimental study, we presented a three-dimensional reconstruction of cranial defects by using trifocal biplanar distraction-compression osteosynthesis (TBDCO).Eight sheep with a mean age of 2 years were divided into 2 groups as the control (n = 3) and distraction groups (n = 5). All the animals were operated on under general anesthesia, and 80 x 50-mm full-thickness calvarial defects were created. In the control group, no treatment was performed. In the distraction group, TBDCO was performed with a custom-made distraction device with 2 transport segments. After 5 days of latency period, the anterior transport segment was distracted to a posterior and superior direction (0.7 and 0.3 mm, respectively) and the posterior transport segment was distracted obliquely to an anterior and superior direction (0.7 and 0.3 mm, respectively) with a resulting total vectorial movement of 0.75 mm/d. Active distraction was approximately 29 days, and the consolidation period was 60 days.No major complications were seen during the whole procedure. In the control group, the bone defects remained unhealed. In the distraction group, defects healed and the reconstructed calvarium was dome shaped. Preoperative and postoperative cranial volume measurements of the distraction group animals revealed an increase in cranial volume (P < 0.05). Histologic evaluation showed inductive bone regeneration and mature bone structure development within the distraction zone. Trifocal biplanar distraction-compression osteosynthesis is an effective and safe technique for three-dimensional closure of the cranial defects. In clinical practice, it could provide several advantages over currently available reconstructive procedures.
The Journal of craniofacial surgery 03/2010; 21(2):483-8. · 0.81 Impact Factor
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Mehmet Bozkurt M.D,
Yalcin Kulahci M.D,
Fatih Zor M.D,
Huseyin Sen M.D,
Cengiz Han Acikel M.D,
Mustafa Deveci M.D,
Murat Turegun M.D,
Mustafa Sengezer M.D,
Mehmet Bozkurt,
Yalcin Kulahci, Fatih Zor,
Huseyin Sen,
Cengiz Han Acikel,
Mustafa Deveci,
Murat Turegun,
Mustafa Sengezer
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ABSTRACT: Background:This experimental study was designed to investigate and compare the effects of different anesthesia techniques on rat cremaster muscle flap microcirculation.Methods:Fifty male Sprague-Dawley rats (130–150 g body weight) were divided into five experimental groups containing ten animals each. Group I, group II, and group III were designated as inhalation, epidural, and spinal anesthesia groups, respectively. Group IV was designated as a combination group for inhalation and epidural anesthesia. Group V was a combination group of inhalation and spinal anesthesia.Results:Group III and group V showed significant increases in the number of rolling and sticking leucocytes and in RBC volume (peripheral stasis) when compared with group I. Blood flow and velocity significantly increased without peripheral stasis in groups II and IV when compared with group I. Although there was no statistically significant difference in the numbers of rolling, sticking, and transmigrating leucocytes or in functional capillary perfusion, group IV had better flow hemodynamics in the peripheral microcirculation when compared with group I.Conclusions:The inhalation and epidural anesthesia combination was determined to be the ideal anesthesia technique for improved peripheral microcirculation. Spinal anesthesia, either separately or in combination with inhalation anesthesia, has adverse effects on microcirculation. © 2009 Wiley-Liss, Inc. Microsurgery, 2010.
Microsurgery 12/2009; 30(1):55 - 60. · 1.61 Impact Factor
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ABSTRACT: In this study, we extended application of face transplantation model in rat by incorporation of vascularized premaxilla, and nose with infraorbital and facial nerves for evaluation of allotransplanted sensory and motor nerve functional recovery. In group I (n = 3) the dissection technique is studied. In group II (n = 5) isotransplantations were performed. In group III (n = 5) allotransplantations were performed under Cyclosporin A monotherapy. Grafts; composed of nose, lower lip, and premaxilla; were dissected. Infraorbital nerve and facial nerve were included into the transplant. A heterotopic transplantation was performed to inguinal region of recipient. Nerve coaptations were performed between infraorbital-sapheneous nerve and facial-femoral nerve. CT scan, somatosensory-evoked potential testing (SSEP), motor-evoked potential testing (MEP), and microangiography were used for evaluation. All transplants survived indefinitely over 100 days. Microangiography showed preserved vascularization of the graft. Computed tomography revealed vital premaxillary bone segments. SSEP and MEP confirmed recovery of motor and sensory functions and latencies reached 67% of normal infraorbital nerve value and 70% of normal facial nerve value at 100 days post-transplant. We have introduced new midface transplant model of composite midface allograft with sensory and motor units. In this model, motor and sensory functional recovery was confirmed at 100 days post-transplant.
Transplant International 12/2009; 23(6):649-56. · 2.92 Impact Factor
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ABSTRACT: This experimental study was designed to investigate and compare the effects of different anesthesia techniques on rat cremaster muscle flap microcirculation.
Fifty male Sprague-Dawley rats (130-150 g body weight) were divided into five experimental groups containing ten animals each. Group I, group II, and group III were designated as inhalation, epidural, and spinal anesthesia groups, respectively. Group IV was designated as a combination group for inhalation and epidural anesthesia. Group V was a combination group of inhalation and spinal anesthesia.
Group III and group V showed significant increases in the number of rolling and sticking leucocytes and in RBC volume (peripheral stasis) when compared with group I. Blood flow and velocity significantly increased without peripheral stasis in groups II and IV when compared with group I. Although there was no statistically significant difference in the numbers of rolling, sticking, and transmigrating leucocytes or in functional capillary perfusion, group IV had better flow hemodynamics in the peripheral microcirculation when compared with group I.
The inhalation and epidural anesthesia combination was determined to be the ideal anesthesia technique for improved peripheral microcirculation. Spinal anesthesia, either separately or in combination with inhalation anesthesia, has adverse effects on microcirculation.
Microsurgery 12/2009; 30(1):55-60. · 1.61 Impact Factor
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ABSTRACT: The aim of this study is to assess 3-dimensional reconstruction of cranial defects by use of bifocal bidirectional transport distraction osteogenesis (BBTDO).
This study was performed on 8 sheep, divided into a control group (n = 3) and treatment group (n = 5). Full-thickness cranial defects (50 x 40 mm) were created on calvaria. In the control group only the skin was closed. In the treatment group BBTDO was performed. Distraction was performed with a custom-made distraction device with a transport segment of 40 x 20 mm. After a 5-day period of latency, distraction was applied to the transport segment. During the first 20 days of distraction, the transport segment was distracted 1 mm in the forward direction and 0.5 mm in the upward direction. After the next 20 days of distraction, the transport segment was distracted 1 mm forward and 0.5 downward. After a total of 40 days' distraction, a 30-day consolidation period was applied. Macroscopic, radiologic (computed tomography with volume measurements), and histologic evaluations were done.
No major complications were seen during the whole study period. In the control group the bone defects remained unhealed at the end of the study period. The same-sized defects in the treatment group healed with a convexity like the calvaria. Preoperative and postoperative cranial volume measurements of the treatment group animals showed an increase in cranial volume (P < .05). Histologic evaluation showed inductive bone regeneration and mature bone structure development within the distraction zone.
The BBTDO is an effective and safe technique for 3-dimensional closure of cranial defects.
Journal of oral and maxillofacial surgery: official journal of the American Association of Oral and Maxillofacial Surgeons 12/2009; 68(4):756-61. · 1.58 Impact Factor
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ABSTRACT: Pain management during burn dressing changes is a critical part of treatment in acute burn injuries. Although several treatment options have been suggested, it is still a challenge in a clinical setting. This study is aimed at finding out an ideal analgesic, sedative and/or anxiolytic combination that would minimise the unwanted effects of ketamine. A total of 24 patients, with burns up to 20-50% of total body surface area (TBSA), were included in the study and randomly divided into three groups. In group I, 2 mg kg(-1) ketamine was administered. In group II, 1 mg kg(-1) tramadol was administered and 30 min later, 1 microg kg(-1) dexmedetomidine and 2 mg kg(-1) ketamine was administered. In group III, 1 mg kg(-1) tramadol was applied and 30 min later, 0.05 mg kg(-1) midazolam and 2 mg kg(-1) ketamine was administered. The evaluation was performed with cardiopulmonary monitoring, sedation and visual analogue pain scores and overall patient satisfaction. Any adverse effects of ketamine were recorded. The results showed that group II had better outcomes with respect to pain management during dressing changes. As a conclusion, the use of the combination of ketamine, tramadol and dexmedetomidine was found to be a good treatment option for the prevention of the procedural pain suffered by adult patients during dressing changes.
Burns: journal of the International Society for Burn Injuries 10/2009; 36(4):501-5. · 1.95 Impact Factor
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ABSTRACT: Atypical mycobacterial tenosynovitis of the wrist can easily be misdiagnosed as synovial chondromatosis. Both sonography and magnetic resonance imaging plays an important role in depicting "rice bodies" within the distended tendon sheaths and bursae of atypical mycobacterial infection. An endemic place for Mycobacterium species and the occupation of the patient should raise the suspicion for the disease. Polymerase chain reaction of the distended tendon fluid is a sensitive, specific and rapid method in identification of the mycobacteria.
Diagnostic and interventional radiology (Ankara, Turkey) 10/2009; 15(4):266-8. · 1.10 Impact Factor
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ABSTRACT: In this study, the authors present their experience with preputial mucosal flap usage for reconstruction of full-thickness skin loss of the penis in a patient with scald injury and discuss the possible treatment modalities in penile skin defects. During the 2-year follow-up period, no complication was encountered, and the patient healed uneventfully.
Urology 08/2009; 74(6):1344-6. · 2.43 Impact Factor
