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Publications (12)0 Total impact

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    ABSTRACT: This report describes the preliminary characterization of a novel antigen reactive with a murine monoclonal antibody designated B1N produced in our laboratory. This antibody (IgM) reacts in IFI with mammals and also insect cells, by staining in a speckled fashion the nucleus of these cells. Immunoblotting analysis of Hela and murine D55 nuclear extracts revealed a polypeptide with an apparent molecular weight of 120kD (p120). In this work we demonstrated that: 1. this polypeptide appeared in human peripheral blood lymphocytes only when they were induced to proliferate in vitro after phytohemagglutinin stimulation; 2. this polypeptide was no longer detected in D55 resting cells, following serum deprivation; 3. the MAb B1N specifically revealed the nucleus of proliferating cells on frozen sections of uterine tissue. These data strongly suggest that the p120 nuclear antigen expression is associated with the proliferation state of cells.
    Comptes Rendus de l Académie des Sciences - Series III - Sciences de la Vie 02/1991; 312(7):301-7.
  • C Crucière · M C Guillemin · A Roseto · A Wirbel · E Plateau ·
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    ABSTRACT: Monoclonal antibodies (Mo Abs) were prepared against influenza/A/equine/Prague/1/56 (H7N7) and influenza/A/equine/Miami/1/63 (H3N8) reference strains of equine influenza virus. These monoclonals were tested against the 2 reference strains, 8 field strains of equine influenza virus, 3 human influenza viruses possessing the H3 hemagglutinin, and one virus of human origin possessing the H1 hemagglutinin. Two antibodies were obtained in one fusion against the Prague/1/56 strain and reacted only with this strain. Four anti/A/equine/Miami/1/63 Mo Abs were obtained in one fusion. They differentiated 8 strains of equine origin from all strains of human origin and from one strain of equine origin (Joinville/1/78) isolated in 1978. The specificity of this difference was confirmed by cross-seroneutralization between A/equine/Miami/1/63 strain and A/equine/Joinville/1/78 strain.
    Annales de recherches vétérinaires. Annals of veterinary research 02/1989; 20(3):243-50.
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    ABSTRACT: A monoclonal antibody (7B10) which displays differential reactivity with breast carcinomas compared to benign lesions or normal breast tissue was selected by fusion of spleen cells from BALB/c mice immunized with the T47D human mammary carcinoma cell line. The antigen, recognized by 7B10 on T47D cells, appeared to be both surface and cytoplasm localized, as demonstrated by indirect immunofluorescence, immunoperoxidase, and electron microscopy studies. This antibody (IgG1) bound with four human breast cancer cell lines (T47D, MCF7, ZR-75-1, and HSL53) which express estrogen receptors. No binding was observed with cancer cell lines of other origin or with normal cells. In vivo, by immunoperoxidase staining of frozen sections of normal breast, the antigen recognized by 7B10 appeared to be located on epithelial cell membranes, whereas in benign and malignant mammary disorders, staining also involved the cytoplasm, as confirmed by electron microscopy on fresh cancer tissue. On formalin-fixed, paraffin-embedded sections, cytoplasmic staining was detected in breast cancer, but no immunostaining was observed with benign lesions or normal breast. In paraffin sections, most normal tissues investigated did not react with 7B10 antibody. However, ducts in the parotid gland, tubules in the kidney and some biliary ducts, and apocrine glands in the skin showed irregular, diffuse weak staining. 7B10 was unreactive with adenocarcinomas of origin other than breast, except for some cells in ovarian clear cell carcinoma. No reactivity was observed with squamous carcinomas, lymphomas, or melanomas. The antigen recognized by 7B10 appeared to be a Mr 32,000 protein, as identified by immunoprecipitation from extracts of T47D after labeling with [35S]methionine. Since the antigen was present only on the membrane of differentiated normal mammary epithelial cells, and was also expressed in the cytoplasm of tumor cells, it may be of interest in immunological studies of mammary epithelial cell differentiation. Moreover, since in formalin-fixed tissues immunostaining is virtually confined to mammary carcinomas, monoclonal antibody 7B10 may have diagnostic applications in breast cancer.
    Cancer Research 09/1987; 47(16):4444-52. · 9.33 Impact Factor
  • G Pancino · C Charpin · F Calvo · M C Guillemin · A Roseto ·
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    ABSTRACT: Two mouse hybridomas, producing the monoclonal antibodies 7B10 and 1BE12 which react with membrane antigens of a metastatic human breast tumor cell line were selected. One of them, 7B10, is directed against a mammary gland antigenic determinant and selectively stain mammary carcinoma on histologic sections after fixation and paraffin embedding.
    Comptes Rendus de l Académie des Sciences - Series III - Sciences de la Vie 02/1987; 304(11):313-8.
  • L Monjour · A Berneman · I Vouldoukis · M Domurado · M C Guillemin · C Chopin · C Alfred · A Roseto ·
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    ABSTRACT: Mice monoclonal antibodies (IgG) have been raised against Leishmania infantum promastigotes by fusing SP 2/0 myeloma cells and immunized mice splenic cells. The monoclonal antibodies have been detected by indirect immunofluorescence. In vivo tests showed that some of them could inhibit the life cycle of several Leishmania species from the Old and the New World. Studies of these protective monoclonals by the western blot technique showed the presence of three constant antigens (40 kD, 70 kD and 113 kD) amongst the Leishmania species studied.
    Comptes Rendus de l Académie des Sciences - Series III - Sciences de la Vie 02/1985; 300(9):395-8.
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    A Roseto · P Pothier · M C Guillemin · J Peries · F Breitburd · N Bonneaud · G Orth ·
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    ABSTRACT: Two stable monoclonal hybridoma cell lines secreting type-specific antibodies against the human papillomavirus type 1 (HPV-1) were isolated. The monoclonal antibodies detected HPV-1 antigens in frozen sections of HPV-1-induced warts, using immunofluorescence or immunoperoxidase techniques, and they reacted with HPV-1 particles in an immunodiffusion test. The two monoclonal antibodies recognized the major structural viral polypeptide, with a molecular weight of 54 000, and a minor polypeptide, with molecular weight of 76 000, in both the dissociated viral particles and in the wart extracts.
    Journal of General Virology 09/1984; 65 ( Pt 8)(8):1319-24. DOI:10.1099/0022-1317-65-8-1319 · 3.18 Impact Factor

  • Hybridoma 02/1984; 3(3):297-300. DOI:10.1089/hyb.1984.3.297
  • M C Mazeron · T Berbar · M C Guillemin · R Colimon · A Roseto · Y Perol ·
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    ABSTRACT: Cell fusion of Sp2/0, a murine myeloma derived non-secreting variant, with splenic lymphocytes from Mice immunized against human cytomegalovirus, has originated two stable hybridomas producing monoclonal antibodies (IgG) which react specifically with antigens that appear early in infectious cycle and remain localized in the cell nucleus.
    Comptes rendus des séances de l'Académie des sciences. Série III, Sciences de la vie 02/1983; 297(6):305-8.
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    A Roseto · R Scherrer · J Cohen · M C Guillemin · A Charpilienne · C Feynerol · J Peries ·
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    ABSTRACT: Five monoclonal hybridoma cell lines secreting antibodies against bovine rotavirus have been produced and four of them characterized by immunostaining of structural polypeptides electrophoretically transferred on to nitrocellulose sheets. Three hybridomas appeared to be directed against the major structural polypeptide (VP39) of the virion. These three monoclonals cross-reacted with the major polypeptide of simian rotavirus and human rotavirus. A fourth hybridoma appeared to react specifically with the high-molecular weight external polypeptide (VP89) and its cleavage products. A cross-reaction was observed with human Wa strain but not with SA11. The fifth hybridoma, even though reacting in an immunofluorescent test, did not show any reactivity by immunostaining. None of the monoclonals neutralized the infectivity of bovine rotavirus.
    Journal of General Virology 02/1983; 64 (Pt 1)(1):237-40. DOI:10.1099/0022-1317-64-1-237 · 3.18 Impact Factor
  • A Roseto · J F Vautherot · P Bobulesco · M C Guillemin ·
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    ABSTRACT: Twelve hybrid cell lines secreting monoclonal immunoglobulins (IgG) specific of bovine enteric Coronavirus (BEC strain G110) have been obtained following fusion of non secreting variant of the myeloma P3X63Ag8, SP2O, which splenic lymphocytes of Mice immunized against this virus. Antibodies thus obtained have been studied by indirect immunofluorescence and seroneutralization with cells infected by two french strains G110 and F15 and an american strain of Coronavirus: NCDCV. Immunochemical studies have confirmed this specificity.
    Comptes rendus des séances de l'Académie des sciences. Série III, Sciences de la vie 03/1982; 294(8):347-52.
  • A Roseto · M C Guillemin · J Cohen · R Scherrer · J Périés ·
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    ABSTRACT: Cell fusion of a murine myeloma derived non secreting variant with splenic lymphocytes from mice immunized against bovin Rotavirus, has originated several hybridomes producing antibovin Rotavirus monoclonal immunoglobulins. These antibodies react as well with both human and simian Rotaviruses.
    Comptes rendus des séances de l'Académie des sciences. Série III, Sciences de la vie 02/1982; 294(4):211-4.
  • A Roseto · F Cavalieri · M C Guillemin · L Dianoux · M Sitbon · F Lema · J Periés ·
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    ABSTRACT: Replication of human rotaviruses on cultured human cells obtained from an intestinal carcinoid tumor was observed by electron microscopy. No enzymatic pretreatment of either the cell monolayers or the inoculum was needed. The characteristics of the vital progeny are discussed.
    Comptes rendus des séances de l'Académie des sciences. Série III, Sciences de la vie 02/1981; 292(2):187-90.