G Steinhoff

University of Rostock, Rostock, Mecklenburg-Vorpommern, Germany

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Publications (117)357.45 Total impact

  • K. J. Oldhafer · J. Fuchs · G. Steinhoff · H. Mildenberger ·
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    ABSTRACT: Für die Resektion von großen kindlichen Lebertumoren in kritischer Lokalisation wurde die Resektion im Kreislaufstillstand und tiefer Hypothermie an der Herz-Lungen-Maschine eingeführt. Wir berichten über unsere Erfahrungen mit Leberresektionen an der Herz-Lungen-Maschine bei 3 Kindern mit Hepatoblastomen. Beim ersten Kind wurde die Resektion im kompletten Kreislaufstillstand durchgeführt, bei den beiden anderen unter Low-flow-Kreislaufbedingungen. Die Phasen an der Herz-Lungen-Maschine wurden von den kleinen Patienten gut vertragen. Es wurden erweiterte rechtsseitige Resektionen mit Gefäßrekonstruktionen vorgenommen. Die postoperativen Leberenzymanstiege waren überraschend niedrig. So lagen die maximalen Werte für die GOT zwischen 100 und 200 U/l. Durch die Resektion konnte in Kombination mit der Chemotherapie ein befriedigendes Langzeitüberleben trotz des ausgedehnten Tumorwachstums erreicht werden. Ein Kind lebt 8 Jahre, ein weiteres 10 Monate ohne Tumorrezidiv. Das 3. Kind verstarb an einer Sepsis während der adjuvanten Chemotherapie, nachdem es sich von der Leberresektion gut erholt hatte. In order to perform resections of tumors at critical sites in the liver in young children, liver resections in cardiac arrest and deep hypothermia under cardiopulmonary bypass have been developed. We report our experience with liver resection under cardiopulmonary bypass in three children with hepatoblastoma. In the first child the operation was performed under cardiac arrest and, the other two children were operated on under „low flow“ conditions. The periods under cardiopulmonary bypass circulation were well tolerated. Extended right liver resections with vascular reconstructions were performed. The postoperative increase of liver enzymes was moderate. The increase in GOT was between 100 and 200 U/l. In spite of the extended tumor growth, reasonable long-term results were achieved by resection in combination with chemotherapy. One child has been living 8 years and another 10 months without tumor recurrence. The third child died due to sepsis during adjuvant chemotherapy, after she had recovered well from liver resection.
    Der Chirurg 04/2012; 71(6):692-695. DOI:10.1007/s001040051121 · 0.57 Impact Factor
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    T. M. Aper · O E Teebken · G Steinhoff · A Haverich ·
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    ABSTRACT: Morphological and functional characterization of cocultured endothelial cells (EC) and myofibroblasts (MFB) seeded on a matrix composed of a fibrin preparation mimicking the microenvironment of a vascular wall. MFB and EC were isolated from human saphenous veins and expanded separately in vitro. MFB were seeded on a composite matrix consisting of a fibrin preparation (with or without transforming growth factor-beta2) and a polyglactin-mesh to form a 3-dimensional structure, which was consecutively reseeded with EC. Seeded matrices were incubated in a bioreactor. Characterization was done including fluorescence staining, live-/dead-assay and immunohistochemistry. High density cocultures in hierarchical structure mimicking the formation of a vascular wall were obtained with nearly complete coverage of the surface with EC. Distribution of preseeded MFB in a 519+/-27 microm thick layer (day 14) was achieved. Cell viability was shown in fluorescence staining for at least 19 days. In deeper layers, no viable cells could be detected within the fibrin preparation. EC covered the surface, had uniform morphology, and their preserved viability was shown for at least 5 days. No EC-ingrowth was found into the fibrin preparation. Neoformation of the matrix proteins laminin and collagen IV was observed. A structured coculture of MFB and EC was obtained mimicking the formation of a vascular wall with preserved viability utilizing a fibrin preparation. Nutrition problems seem to limit the maximal extent of MFB in the matrix.
    European Journal of Vascular and Endovascular Surgery 10/2004; 28(3):296-302. DOI:10.1016/j.ejvs.2004.05.016 · 2.49 Impact Factor
  • Michael Brandt · Christian Grotkop · Jörg Steinmann · Gustav Steinhoff ·
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    ABSTRACT: Infiltration of leukocytes into the lung allograft is regulated by adhesion molecules during acute rejection. The purpose of this study was to assess the effect of monoclonal antibodies against ICAM-1 (1A29) to prevent rejection after lung transplantation. Left lateral orthotopic lung transplantation was performed using Dark Agouti rats as donors and Lewis rats as recipients. Recipients received 1A29 alone (group A), cyclosporine A alone (group B), a combination of both drugs (group C) or no therapy (group D). Animals were killed on day 5 and 10, respectively. Rejection was graded by histology. Myeloperoxidase activity (MPO) was measured in the graft. In allografts treated with cyclosporine and 1A29 histologically a lower grade of rejection was seen and less MPO were detected compared to groups A, B and D. Anti-ICAM-1 monoclonal antibodies alone as well as a subtherapeutic dose of cyclosporine are not effective to prevent acute allograft rejection after lung transplantation. However, the combination of both strategies significantly reduces rejection in this model.
    Interactive Cardiovascular and Thoracic Surgery 01/2004; 2(4):509-13. DOI:10.1016/S1569-9293(03)00131-2 · 1.16 Impact Factor
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    ABSTRACT: The discovery that pig endogenous retroviruses are infectious for human cells in vitro lead to vehement discussions about the possible risk of infection after clinical xenotransplantation. Since PERV transmission to non-human primate cells in vitro has been observed, similar to human cells, infection studies in non-human primates should represent the best model to analyze a potential PERV transmission after xenotransplantation. However, it is still open to discussion, whether non-human primate cells can be infected productively-similar to human cells- and whether those species are suitable to analyze PERV infection risks in vivo. In vitro, only few cell types can be tested for susceptibility. We developed a pig to baboon cell transplantation model with special emphasis on B-cell effective immunosuppression, removal of anti Gal-alpha 1,3-Gal-antibodies, inhibition of the complement cascade and long term survival of transplanted cellular grafts. This model allows us to investigate in vivo, whether any baboon cell types may be permissive for productive PERV infection. The xenograft recipients were investigated for up to 535 days post transplantation. Gal-alpha 1,3-Gal-antibody and complement levels were monitored. Potential PERV transmission was analyzed, not only in PBMC, but in a variety of tissue samples as well as in serum and plasma samples by PCR, RT-PCR and by detection of RT-activity. Moreover, potential PERV specific immune responses were studied by a highly sensitive Western-Blot-assay. Despite several days of extremely low levels of Gal-alpha 1,3-Gal-antibody and complement, and despite of long term xenochimerism, no evidence for PERV infection was obtained in any of the tested tissues or in the tested serum samples. This study supplies further evidence for a low susceptibility of baboons towards productive PERV infection after xenotransplantation.
    Annals of transplantation: quarterly of the Polish Transplantation Society 02/2003; 8(3):24-34. · 1.26 Impact Factor
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    ABSTRACT: Xenotransplantation represents a promising solution to the ever increasing shortage of donor organs in allotransplantation. However, due to different and stronger modes of rejection, successful xenotransplantation will require different organ-protective regimes from those used in allogeneic transplantation today. Since one can not simply increase the dosage of the drugs used, immunomodulation or tolerance induction of the recipient would be the most desirable approach. Transfusion of donor leukocytes has been shown to downregulate recipient responses or even induce peripheral tolerance in small animal models. Since the infusion of donor cells represents a relatively simple approach, as one can purify and compose the inoculum exactly before infusion, we studied whether this approach can be successfully employed in a preclinical swine to non-human primate model of peripheral tolerance induction/immunomodulation. In our model, baboons underwent sequential column adsorption and complement blockade. The animals received only initial immunosuppression with cyclophosphamide. No further immunosuppression was given. Subsequently all animals received 1-3 x 10(10) porcine splenocytes i.v. Development and maintenance of chimerism was analyzed by sequential flow cytometric and PCR analyses. Other parameters studied included effects of the preparatory induction protocol. We could show that a low level of chimerism is maintained in these animals for up to 1.5 years, despite the fact that they received no additional immunosuppression after the initial one. At no time of the experiment did any animal display symptoms of poor health. Thus we demonstrate that the concept of donor leukocyte transfusion is transferable into preclinical xenotransplantation. We are currently conducting organ transplantation experiments into animals thus treated to directly analyze the immunomodulatory effect of the donor cells.
    Annals of transplantation: quarterly of the Polish Transplantation Society 02/2002; 7(3):40-5. · 1.26 Impact Factor

  • The Journal of Heart and Lung Transplantation 01/2002; 21(1):120-121. DOI:10.1016/S1053-2498(01)00616-7 · 6.65 Impact Factor
  • C Hagl · U Stock · A Haverich · G Steinhoff ·
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    ABSTRACT: A variety of minimally invasive techniques have been recently introduced in adult cardiac surgery. Experiences with children and newborns are, however, limited. In this report, we present our first experiences with different methods of ministernotomies for closure of atrial septum defect (ASD) and ventricular septum defect (VSD) in pediatric cardiac patients. Also, the current literature for different surgical approaches is reviewed. Twenty-five pediatric patients (range, 4 months to 12 years old) underwent elective ASD or VSD closure. Surgical access was either without division of the sternum (group A, n = 5), with partial inferior sternotomy (group B, n = 5), total sternotomy with limited skin incision (group C, n = 5), or total sternotomy with full skin incision (group D, n = 10). There were no severe intraoperative complications regarding exposure, cannulation, or bleeding. Conversion to full sternotomy was not necessary in any patient. Bypass time and cross-clamp time in groups A, B, and C were comparable to the standard operation (group D). However, preparation time was significantly increased in one minimally invasive group (group A vs group D, p<0.05). Despite general feasibility, the transxiphoidal access without sternotomy compromises exposure of the ascending aorta, resulting in impaired administration of cross-clamping, cardioplegia, and especially de-airing. Transatrial pediatric cardiac operations can be performed without or with limited sternotomy. The partial sternotomy allows uncompromised exposure of the great vessels and should be favored over the transxiphoidal approach. The operative access and perioperative risk is comparable to a classical standard surgical approach. Advantages include improved cosmetic results in combination with a high degree of safety.
    Chest 02/2001; 119(2):622-7. DOI:10.1378/chest.119.2.622 · 7.48 Impact Factor
  • K Wiebe · J Poeling · R Meliss · M Loss · M Winkler · G Steinhoff · A Haverich ·

    Transplantation Proceedings 02/2001; 33(1-2):773-4. DOI:10.1016/S0041-1345(00)02247-8 · 0.98 Impact Factor
  • C Schröder · U Martin · A Simon · G Laaff · K Wiebe · A Haverich · B Lapin · G Steinhoff ·

    Transplantation Proceedings 02/2001; 33(1-2):738-9. DOI:10.1016/S0041-1345(00)02230-2 · 0.98 Impact Factor

  • Transplantation Proceedings 02/2001; 33(1-2):692. DOI:10.1016/S0041-1345(00)02206-5 · 0.98 Impact Factor
  • R Tessmann · G Steinhoff · U Martin · A Haverich · AR Simon ·

    Transplantation Proceedings 02/2001; 33(1-2):792. DOI:10.1016/S0041-1345(00)02258-2 · 0.98 Impact Factor
  • AR Simon · C Schröder · U Martin · M Chikobava · C Templin · G Laaf · K Wiebe · B Lapin · A Haverich · G Steinhoff ·

    Transplantation Proceedings 02/2001; 33(1-2):705. DOI:10.1016/S0041-1345(00)02213-2 · 0.98 Impact Factor
  • Source
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    ABSTRACT: The question whether porcine xenografts can lead to porcine endogenous retrovirus (PERV) infection of recipients is critical for the evaluation of the safety of pig-to-man xenotransplantation. Unfortunately, polymerase chain reaction (PCR)-based analysis of potential PERV infections in nonhuman-primate whole-organ xenotransplantation models is hampered by false positive results due to chimeric porcine cells. To avoid the inherent analytical problem of xenomicrochimerism, we developed a non-life-supporting pig-to-primate kidney xenotransplantation model: porcine kidneys were transplanted, whereas the functioning recipient kidneys remained in situ. Subsequent to rejection (after 2 hours to 15 days), xenografts were removed, and recipients remained alive for up to 287 days. Immunosuppressive therapy based on cyclophosphamide, cyclosporine, and steroids was maintained for 28 days after transplantation. Using appropriate PCR assays, xenochimerism was found in tissue samples and partly even in peripheral blood leukocytes (PBLs) while the porcine kidneys were in situ. After graft removal, xenochimerism was no longer detectable, thus allowing analysis for possible PERV transmission.
    Transplant International 02/2001; 14(1):31-7. DOI:10.1007/s001470050739 · 2.60 Impact Factor
  • A Lührmann · R V Haberberger · C Mörike · G Steinhoff · N Krug · R Pabst · T Tschernig ·
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    ABSTRACT: It is not clear whether surgical intervention during lung transplantation which includes cutting vegetative nerves, lymphatic vessels and bronchial arteries, leads to alterations in immune responses. Thus, it was studied in an animal model whether an induced pulmonary immune reaction after syngenic lung transplantation was impaired without the influence of immunosuppression and rejection. The recruitment of leukocytes and the status of reinnervation was examined. Syngenic transplantation of the left lung was performed in Lewis rats without rejection and therefore without immunosuppressive therapy. In a subgroup of animals host and donor leukocytes were distinguished. An ovalbumin (OVA)-specific pulmonary immune response was induced four months after transplantation. Bronchoalveolar lavage (BAL) and interstitial leukocytes were examined using flow cytometry and immunocytology, comparing the right lung and the grafted left lung. Immunohistology was performed to detect nerve fibers on cryostat sections. An induced cellular inflammation was observed in the right host lung as well as in the grafted left lung. However, the CD4 T cell numbers in the BAL were increased in the left lung. Single donor-type leukocytes could still be observed four months after transplantation. A partial reinnervation was found. The recruitment of immune cells into the lung interstitium and bronchoalveolar space of grafted lungs is not impaired. The incomplete reinnervation has no influence on leukocyte recruitment.
    Pneumologie 01/2001; 54(12):564-8.
  • G Steinhoff · U Stock · N Karim · H Mertsching · A Timke · R R Meliss · K Pethig · A Haverich · A Bader ·
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    ABSTRACT: Tissue engineering using in vitro-cultivated autologous vascular wall cells is a new approach to biological heart valve replacement. In the present study, we analyzed a new concept to process allogenic acellular matrix scaffolds of pulmonary heart valves after in vitro seeding with the use of autologous cells in a sheep model. Allogenic heart valve conduits were acellularized by a 48-hour trypsin/EDTA incubation to extract endothelial cells and myofibroblasts. The acellularization procedure resulted in an almost complete removal of cells. After that procedure, a static reseeding of the upper surface of the valve was performed sequentially with autologous myofibroblasts for 6 days and endothelial cells for 2 days, resulting in a patchy cellular restitution on the valve surface. The in vivo function was tested in a sheep model of orthotopic pulmonary valve conduit transplantation. Three of 4 unseeded control valves and 5 of 6 tissue-engineered valves showed normal function up to 3 months. Unseeded allogenic acellular control valves showed partial degeneration (2 of 4 valves) and no interstitial valve tissue reconstitution. Tissue-engineered valves showed complete histological restitution of valve tissue and confluent endothelial surface coverage in all cases. Immunohistological analysis revealed cellular reconstitution of endothelial cells (von Willebrand factor), myofibroblasts (alpha-actin), and matrix synthesis (procollagen I). There were histological signs of inflammatory reactions to subvalvar muscle leading to calcifications, but these were not found in valve and pulmonary artery tissue. The in vitro tissue-engineering approach using acellular matrix conduits leads to the in vivo reconstitution of viable heart valve tissue.
    Circulation 12/2000; 102(19 Suppl 3):III50-5. DOI:10.1161/01.CIR.102.suppl_3.III-50 · 14.43 Impact Factor
  • C Kühn · R Tessmann · C DallaRiva · M Glaubitz · U Martin · G Steinhoff · A Haverich · AR Simon ·

    Transplantation Proceedings 09/2000; 32(5):1043-4. DOI:10.1016/S0041-1345(00)01110-6 · 0.98 Impact Factor
  • G Laaf · S Schuster · U Martin · G Steinhoff · A Haverich · AR Simon ·

    Transplantation Proceedings 09/2000; 32(5):867-8. DOI:10.1016/S0041-1345(00)01016-2 · 0.98 Impact Factor
  • K Wiebe · G Steinhoff · J Poeling · C Schröder · R Meliss · M Winkler · A Haverich ·

    Transplantation Proceedings 09/2000; 32(5):1149-50. DOI:10.1016/S0041-1345(00)01161-1 · 0.98 Impact Factor
  • C Templin · C Schröder · AR Simon · G Laaff · J Köhl · M Chikobava · B Lapin · G Steinhoff · U Martin ·

    Transplantation Proceedings 09/2000; 32(5):1163-4. DOI:10.1016/S0041-1345(00)01167-2 · 0.98 Impact Factor
  • U Martin · M E Winkler · M Id · H Radecke · L Arseniev · R Grotelüschen · AR Simon · G Steinhoff ·

    Transplantation Proceedings 09/2000; 32(5):1157. DOI:10.1016/S0041-1345(00)01164-7 · 0.98 Impact Factor

Publication Stats

3k Citations
357.45 Total Impact Points


  • 2000-2004
    • University of Rostock
      Rostock, Mecklenburg-Vorpommern, Germany
  • 1988-2003
    • Hannover Medical School
      • • Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO)
      • • Department of Gastroenterology, Hepatology and Endocrinology
      Hannover, Lower Saxony, Germany
  • 1994-1998
    • Christian-Albrechts-Universität zu Kiel
      • UKSH Klinik für Herz- und Gefäßchirurgie
      Kiel, Schleswig-Holstein, Germany
  • 1992-1993
    • The University of Tokyo
      • Department of Surgical Sciences
      白山, Tōkyō, Japan