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ABSTRACT: The ubiquitously expressed hypoxia-inducible factor-1α (HIF-1α) acts as a key transcription factor in regulating metabolism, development, cellular survival, proliferation and pathology under hypoxia condition. Compared to mammals, fish are more vulnerable to hypoxia stress and contamination; however, the regulation of HIF-1α in fish remains obscure. In this study, zebrafish HIF-1α promoter was cloned and found to possess a CpG island located at -97 to +403, but the canonical TATA-box was absent. Aligning 240-bp HIF-1α proximal promoter region of zebrafish with other vertebrates showed more than 82 % identity with cyprinid fishes. Further luciferase analysis suggested that the minimal core promoter might locate at -134 to +97, and several putative transcription factor binding sites were found in this region by bioinformatic analysis. Moreover, it was shown that the zebrafish HIF-1α mRNA was significantly activated by 10 μg/mL lipopolysaccharide (LPS) under hypoxia condition and peaked at 8 h after treatment, suggesting LPS- and hypoxia-regulated zebrafish HIF-1α transcriptional activity in a synergistic pattern. This synergistic effect was closely related to the living environment of fish, indicating that this mechanism would be more conducive to fish survival.
Fish Physiology and Biochemistry 02/2013; · 1.53 Impact Factor
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Chengda Zhang,
Wei Wang,
Mengyu Zhou,
Yang Han,
Jing Xie,
Zhifeng Qiu,
Fuping Guo,
Yanling Li, Huanling Wang,
Khalil G Ghanem,
Taisheng Li
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ABSTRACT: OBJECTIVES:: Nevirapine (NVP), a still widely used non-nucleoside reverse-transcriptase inhibitor (NNRTI), can cause severe hepatotoxicity. Previous studies suggest that CD4 cell counts over 250 cells/μL in women and over 400 cells/μL in men are risk factors for NVP-related hepatotoxicity. These studies have informed Chinese national treatment guidelines. We evaluate whether current Chinese guidelines for NVP use are appropriate. METHODS:: Longitudinal data were pooled from 2 clinical trials between 2005 and 2009 across mainland China. 566 antiretroviral-therapy naïve Chinese patients were given NVP-containing ART for 24 weeks. Hepatotoxicity was defined as alanine aminotransferase, aspartate transaminase or total bilirubin level greater than 1.25 times the upper limit of normal range (ULN). Severe hepatotoxicity was defined as greater than 5 times the ULN. RESULTS:: 197 (36.1%) patients developed hepatotoxicity during treatment, including 42 (7.7%) patients with severe hepatotoxicity. CD4 cell count over 250 cells/μL was an independent predictor for hepatotoxicity both in men (Relative risk (RR)=1.22 (95% confidential interval (CI), 1.04-1.44)) and in women (RR=1.72 (95%CI, 1.20-2.46)). Severe hepatotoxicity was also more common among all persons with CD4 >250 cells/μL. CONCLUSIONS:: Hepatotoxicity was a common adverse effect of NVP among men and women with CD4>250 cells/μL. Chinese treatment guidelines should be considered to reflect this risk.
JAIDS Journal of Acquired Immune Deficiency Syndromes 01/2013; · 4.43 Impact Factor
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ABSTRACT: OBJECTIVE:: To evaluate the influence of hepatitis B virus (HBV) co-infection on immunological, virological and clinical responses to lamivudine-based combined antiretroviral therapy (cART) in Chinese patients. DESIGN AND METHODS:: This prospective, multicenter cohort study recruited 529 antiretroviral-naïve participants (aged 18-65 years, both genders) between 2008 and 2010. They were grouped by HBV serostatus. Virological and immunological responses were monitored at baseline and week 4, 8, 12, 24, 36 and 48. cART for all patients was nevirapine, lamivudine with either zidovudine or stavudine. RESULTS:: (1)HIV/HBV co-infection rate in our cohort was 14.6%. (2)Among 508 patients with complete baseline information, median CD4 level was significantly lower in chronic HBV-infected (CHB) group and isolated-core group. In CHB group, HBeAg positivity rather than HBV DNA level was associated with lower CD4 count. (3)In isolated-core group, occult infection rate was 9.5%. (4)At week 48, rate of HIV suppression below 40 copies/mL was 74.2%. Median increase in CD4 at week 48 was 127cells/μL. Of note, HBV serostatus did not influence virological and immunological response to cART at each follow-up time point. Although HBV serostatus was associated with different ALT level during follow-up, hepatitis and hyperbilirubinemia rates were not significantly different. (5)3TC-based regimen was efficacious against HBV replication, with median decrease of HBV DNA 2.87 log copies/mL. However, HBeAg positivity was associated with poorer HBV DNA suppression. CONCLUSION:: In our cohort, chronic HBV infection and isolated HBcAb positivity were related to faster HIV progression. Despite of this, virological and immunological responses were not affected by HBV serostatus.
AIDS 05/2012; · 6.24 Impact Factor
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ABSTRACT: Eleven microsatellite markers were isolated and characterized from enriched genomic libraries of Channa argus. The loci were scored in 40 specimens from one natural population. We explored 6–17 alleles per locus with observed and expected
heterozygosities ranging from 0.8000 to 1.0000 and 0.7269 to 0.9551, respectively, containing 0.830 average polymorphic information
content. Significant deviations from Hardy–Weinberg equilibrium were detected only at one loci and linkage disequilibrium
between three loci was found to be significant after applying Bonferroni correction (P<0.005). These newly developed markers should be significant in population genetic assessment studies or construction of
genetic linkage map for this important snakehead fish species in future.
Keywords
Channa argus
-Microsatellites-Simple sequence repeats-Genomic library
Conservation Genetics Resources 05/2012; 2:59-61. · 0.49 Impact Factor
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ABSTRACT: To obtain the phylogenetic relationship between diploid and tetraploid Misgurnus anguillicaudatus, the mitochondrial cyt b gene in the diploid and tetraploid weather loach were isolated and sequenced. The DNA sequences were analyzed using MEGA
3.0 software to determine the phylogenetic relationship. Forty-five variable sites among cyt b gene sequences and 18 amino acid substitutions occurred within the diploid and tetraploid loaches as deduced from the nucleotide
sequences analysis of the cyt b gene. The nucleotide pairwise distance between diploid and tetraploid loach ranged from 0.001 to 0.025. Phylogenetic analysis
revealed evolutionary relationships between diploid and tetraploid loach. Our results indicated a significant difference between
diploid and tetraploid loach about the cyt b gene. AMOVA analysis indicated that there were no significant genetic variations within diploid loaches (Fst = 0.2529, P > 0.05) and within tetraploid loaches (Fst = 0.0564, P > 0.05), neither. However, significant genetic differences were found between diploid and tetraploid loaches (Fst = 0.7634,
P < 0.05). Thus, it is concluded that no reproductive isolation was found within the same cytotypes of different localities,
but there was reproductive isolation between these two cytotypes. The diploid loach existed before the tetraploid loach in
nature. The present study is the first to describe the phylogenetic relationships of natural polyploidy weather loach using
mtDNA cyt b gene.
Environmental Biology of Fishes 04/2012; 86(1):145-153. · 0.91 Impact Factor
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Chenggang Li,
Penghui Yang,
Yang Sun,
Taisheng Li,
Chen Wang,
Zhong Wang,
Zhen Zou,
Yiwu Yan,
Wei Wang,
Zhongwei Chen, [......],
Jiejie Deng,
Yan Zhao,
Peng Yang,
Jun Tang, Huanling Wang,
Zhongpeng Zhao,
Zhinan Yin,
Bin Cao,
Xiliang Wang,
Chengyu Jiang
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ABSTRACT: The 2009 flu pandemic involved the emergence of a new strain of a swine-origin H1N1 influenza virus (S-OIV H1N1) that infected almost every country in the world. Most infections resulted in respiratory illness and some severe cases resulted in acute lung injury. In this report, we are the first to describe a mouse model of S-OIV virus infection with acute lung injury and immune responses that reflect human clinical disease. The clinical efficacy of the antiviral oseltamivir (Tamiflu) administered in the early stages of S-OIV H1N1 infection was confirmed in the mouse model. Moreover, elevated levels of IL-17, Th-17 mediators and IL-17-responsive cytokines were found in serum samples of S-OIV-infected patients in Beijing. IL-17 deficiency or treatment with monoclonal antibodies against IL-17-ameliorated acute lung injury induced by the S-OIV H1N1 virus in mice. These results suggest that IL-17 plays an important role in S-OIV-induced acute lung injury and that monoclonal antibodies against IL-17 could be useful as a potential therapeutic remedy for future S-OIV H1N1 pandemics.
Cell Research 03/2012; 22(3):528-38. · 8.19 Impact Factor
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ABSTRACT: Histone deacetylase 4 (HDAC4) is an important modifier enzyme for chromatin remodeling and plays an essential role in regulating gene expression. Spatio-temporal expression spectrum revealed that zebrafish hdac4 mRNA, ubiquitously distributed in various tissues, were significantly higher at 36 hpf (hours post-fertilization) and 6 dpf (days post-fertilization) than other periods. Trichostatin A (TSA) inhibited the development of zebrafish embryos and transcription of hdac4 and mef2a (myocyte enhancer factor-2A). Moreover, five vectors containing different promoter regions of hdac4 were constructed in order to analyze promoter activity. The vector containing the region from -125 to +160 exhibited maximum luciferase activity that was approximately 30.3-fold and 58.9-fold higher than the control in two kinds of cells, respectively. By comparing the luciferase activities between the region from -302 to +30 and -698 to +30, it was suggested that the region between -698 and -302 might contain mild negative regulatory elements.
Fish Physiology and Biochemistry 07/2011; 38(2):585-93. · 1.53 Impact Factor
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ABSTRACT: Abstract
Background
In a previous screen to identify differentially expressed genes associated with embryonic development, the porcine PNAS-4 gene had been found. Considering differentially expressed genes in early stages of muscle development are potential candidate genes to improve meat quality and production efficiency, we determined how porcine PNAS-4 gene regulates meat production. Therefore, this gene has been sequenced, expression analyzed and associated with meat production traits.
Results
We cloned the full-length cDNA of porcine PNAS-4 gene encoding a protein of 194 amino acids which was expressed in the Golgi complex. This gene was mapped to chromosome 10, q11–16, in a region of conserved synteny with human chromosome 1 where the human homologous gene was localized. Real-time PCR revealed that PNAS-4 mRNA was widely expressed with highest expression levels in skeletal muscle followed by lymph, liver and other tissues, and showed a down-regulated expression pattern during prenatal development while a up-regulated expression pattern after weaning. Association analysis revealed that allele C of SNP A1813C was prevalent in Chinese indigenous breeds whereas A was dominant allele in Landrace and Large White, and the pigs with homozygous CC had a higher fat content than those of the pigs with other genotypes ( P < 0.05).
Conclusion
Porcine PNAS-4 protein tagged with green fluorescent protein accumulated in the Golgi complex, and its mRNA showed a widespread expression across many tissues and organs in pigs. It may be an important factor affecting the meat production efficiency, because its down-regulated expression pattern during early embryogenesis suggests involvement in increase of muscle fiber number. In addition, the SNP A1813C associated with fat traits might be a genetic marker for molecular-assisted selection in animal breeding.
BMC Genetics. 01/2008;
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ABSTRACT: In a previous screen to identify differentially expressed genes associated with embryonic development, the porcine PNAS-4 gene had been found. Considering differentially expressed genes in early stages of muscle development are potential candidate genes to improve meat quality and production efficiency, we determined how porcine PNAS-4 gene regulates meat production. Therefore, this gene has been sequenced, expression analyzed and associated with meat production traits.
We cloned the full-length cDNA of porcine PNAS-4 gene encoding a protein of 194 amino acids which was expressed in the Golgi complex. This gene was mapped to chromosome 10, q11-16, in a region of conserved synteny with human chromosome 1 where the human homologous gene was localized. Real-time PCR revealed that PNAS-4 mRNA was widely expressed with highest expression levels in skeletal muscle followed by lymph, liver and other tissues, and showed a down-regulated expression pattern during prenatal development while a up-regulated expression pattern after weaning. Association analysis revealed that allele C of SNP A1813C was prevalent in Chinese indigenous breeds whereas A was dominant allele in Landrace and Large White, and the pigs with homozygous CC had a higher fat content than those of the pigs with other genotypes (P < 0.05).
Porcine PNAS-4 protein tagged with green fluorescent protein accumulated in the Golgi complex, and its mRNA showed a widespread expression across many tissues and organs in pigs. It may be an important factor affecting the meat production efficiency, because its down-regulated expression pattern during early embryogenesis suggests involvement in increase of muscle fiber number. In addition, the SNP A1813C associated with fat traits might be a genetic marker for molecular-assisted selection in animal breeding.
BMC Genetics 01/2008; 9:40. · 2.47 Impact Factor
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ABSTRACT: The purpose of this study was to determine whether diploid, triploid and tetraploid loach (Misgurnus anguillicaudatus) differed in terms of their main haematological and physiological characteristics. Diploid and tetraploid fish were produced by crossing of natural diploids (2n x 2n) and natural tetraploids (4n x 4n), respectively. Triploid fish were produced by hybridization between diploid males and tetraploid females. The blood cells were significantly larger in polyploids, and the volumetric ratios of erythrocytes and leucocytes (thrombocyte and neutrophil) in tetraploids, triploids and diploids were consistent with the ploidy level ratio of 4:3:2. No significant differences were observed in haematocrit among polyploids. The erythrocyte count decreased with increased ploidy level, while total haemoglobin, mean cell volume, mean cellular haemoglobin content, and mean cell haemoglobin concentration all increased with increase in ploidy level. Erythrocyte osmotic brittleness declined in polyploids so that polyploid erythrocytes were more resistant to osmotic stress than diploid ones. Overall, loach with higher ploidy levels showed evidence of some advantages in haematological characteristics.
Comparative Biochemistry and Physiology - Part A Molecular & Integrative Physiology 09/2007; 147(4):1001-8. · 2.23 Impact Factor
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ABSTRACT: A full-length cDNA clone encoding the eukaryotic translation initiation factor 4A, isoform 2 (EIF4A2), was cloned from the fetal skeletal cDNA library from the pig (Sus scrofa). EIF4A2 is a highly conserved gene for one of the protein-synthesis initiation factors involved in the binding of mRNA to the ribosome. Based on this cDNA sequence, the deduced protein of 407 amino acids contains the characteristic motifs shared by the DEAD-box supergene family. The genomic nucleotide sequence of this gene was determined and a single nucleotide polymorphism located in the 5' untranslated region was genotyped. The porcine EIF4A2 was expressed in all tissues examined but in variable amounts. The EIF4A2 expression level in muscle was upregulated through embryonic and neonatal development until adult, suggesting that porcine EIF4A2 was possibly involved in translation regulation of other muscle-related genes in muscle formation and development. In addition, we mapped porcine EIF4A2 to q4.1 of SSC13, in agreement with comparative mapping data.
Biochemical Genetics 03/2007; 45(1-2):51-62. · 0.86 Impact Factor
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ABSTRACT: Epithelial protein lost in neoplasm (EPLIN) is a cytoskeleton-associated protein that is down-regulated in transformed cells. Two EPLIN isoforms (alpha and beta) are generated by alternative promoter usage from a single gene. In pigs EPLIN was preferentially expressed in the muscle of splay-legged piglets, but not in the healthy ones, suggesting that it plays an important role in muscle development. A precise mRNA expression analysis through muscle development could shed some light on the EPLIN function associated with splayed legs. This article describes the isolation of the two alternative splice variants of EPLIN mRNA in pigs. The chromosome assignment and several polymorphism sites were also identified to lay a foundation for potential breeding applications. Gene expression analysis by real-time polymerase chain reaction (PCR) showed that both of transcripts were expressed in almost all tissues examined but in variable amounts in adult pigs. The temporal expression analysis indicated that they are not coexpressional through muscle development: EPLIN-alpha was detected in developing skeletal muscle, but EPLIN-beta was not.
Animal Biotechnology 02/2007; 18(2):101-8. · 0.93 Impact Factor
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ABSTRACT: Calsarcins comprise a novel family of muscle-specific calcineurin-interacting proteins and play an important role in modulating both the function and substrate specificity of calcineurin in muscle cells. In this study, we cloned and characterized calsarcins from pig muscle. The deduced amino acid sequences of porcine calsarcin-1 (CS-1), calsarcin-2 (CS-2), and calsarcin-3 (CS-3) share the same putative calcineurin and alpha-actinin binding regions. Radiation hybrid mapping data indicate that CS-1 and CS-2 map to q2.1-2.5 of pig chromosome 8 (SSC8) and q2.4 of pig chromosome 14 (SSC14), respectively. The mRNA expressions of both CS-1 and CS-2 are regulated in skeletal muscle similarly during postnatal development but not during prenatal development, indicating differences in function, additionally demonstrated by minute differences in cellular localization within Pig Kidney Epithelial cells (PK15). We provide the first evidence that CS-1 is abundantly expressed in porcine heart and has an expression pattern similar to the human gene. This result suggests that the pig may be a suitable animal model to study the function of calsarcins in human heart disease.
Gene 07/2006; 374:104-11. · 2.34 Impact Factor
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Taisheng Li,
Zhifeng Qiu,
Linqi Zhang,
Yang Han,
Wei He,
Zhengyin Liu,
Xiaojun Ma,
Hongwei Fan,
Wei Lu,
Jing Xie, Huanling Wang,
Guohua Deng,
Aixia Wang
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ABSTRACT: This report demonstrates that a rapid decrease of peripheral T cell subsets is a unique characteristic in patients with SARS during acute infection, although total white blood cell counts, red blood cell counts, and platelet counts remain relatively normal. In recovering patients, a rapid and dramatic restoration of peripheral T cell subsets was seen in the periphery. Although the underlying mechanism of the acute decrease of peripheral T cell subsets observed in patients with SARS during the acute stage remains unknown, this clinical characteristic can facilitate an earlier and more accurate diagnosis of SARS.
The Journal of Infectious Diseases 03/2004; 189(4):648-51. · 6.41 Impact Factor
