L P Sycheva

Russian Academy of Medical Sciences, Moskva, Moscow, Russia

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Publications (28)13.07 Total impact

  • A A Altaeva, L P Sycheva, N N Belyaeva
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    ABSTRACT: Experimental mutagenic effect of acrylamide on thyroid gland cells was studied by an extended micronucleus test. Acrylamide in doses corresponding to 0.004-0.1 LD(50) increased the incidence of thyroid follicular cells (A-cells) with micronuclei and other karyological parameters in exposed rats after hemithyroidectomy. This cytogenetic effect allows regarding acrylamide as a mutagen for the thyroid gland and as a carcinogen for this organ.
    Bulletin of Experimental Biology and Medicine 12/2011; 152(2):275-7. · 0.34 Impact Factor
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    ABSTRACT: Titanium dioxide is manufactured worldwide in large quantities for use in a wide range of applications including as food additives, in cosmetics and pigments for coloring ingested and externally applied drugs. Although TiO(2) is chemically inert it can cause negative health effects, such as lung cancer in rats. However, the mechanisms involved in TiO(2)-induced genotoxicity and carcinogenicity have not been clearly defined and are poorly studied in vivo. In the present research genotoxicity and carcinogenicity of titanium dioxide were studied in a mouse model. We treated CBAB6F1 mice by oral gavage with titanium dioxide particles (microsized, TDM, 160nm; nanosized, TDN, 33nm) in doses of 40, 200 and 1000mg/kg bw, daily for seven days. Genotoxic effects were analyzed in the cells of brain, liver and bone marrow by means of the Comet assay and in the cells of bone marrow, forestomach, colon and testis with a poly-organ karyological assay (analysis of micronuclei, nuclear protrusions, atypical nuclei, multinucleated cells, mitotic and/or apoptotic index). TDM induced DNA-damage and micronuclei in bone-marrow cells and TDN induced DNA-damage in the cells of bone marrow and liver. TDM and TDN increased the mitotic index in forestomach and colon epithelia, the frequency of spermatids with two and more nuclei, and apoptosis in forestomach (only TDN) and testis. This is one of the first poly-organ studies of TDM- and TDN-induced genotoxicity in vivo in mice. These effects are caused by a secondary genotoxic mechanism associated with inflammation and/or oxidative stress. Given the increasing use of TiO(2) nanoparticles, these findings indicate a potential health hazard associated with exposure to TiO(2) particles.
    Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 08/2011; 726(1):8-14. · 3.90 Impact Factor
  • L P Sycheva, V S Zhurkov
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    ABSTRACT: Problems pertaining to the estimation of genetic safety of nanomaterials are considered. Comparative analysis of current approaches to testing mutagenicity in this country and abroad is presented. The necessity of mandatory evaluation of genotoxic and mutagenic properties of modern nanomaterials in accordance with international standards is substantiated. The system of the existing methods for the purpose should be supplemented by new scientifically sound and verified techniques. Methodological peculiarities of the assessment of organ-specific nanomaterials are described. It is recommended to correct certain provisions of the approved Guidelines 1.2.2520-09 concerning mutagenic properties of nanomaterials.
    Vestnik Rossiĭskoĭ akademii meditsinskikh nauk / Rossiĭskaia akademiia meditsinskikh nauk 01/2011;
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    ABSTRACT: The genetic safety of titanium dioxide (TD)-containing foods and cosmetic products has been little investigated. The study evaluated the mutagenic activity of TD in the micronucleus test with animal visceral mucosal epithelial cells. Two simethicone-coated anatase samples (mean size 160 and 33.2 nm) were inserted into the mouse stomach in doses of 40-200-1000 mg/kg seven times and applied as an ingredient of 10 and 25% cream (doses 250 and 625 mg/kg, respectively) to the hair-sheared rat skin once for 4 hours. Analysis of cytogenetic disorders (micronuclei, protrusions, and the atypical form of the nucleus) revealed no mutagenic properties of TD on the mucosal epithelium of the mouse and rat intestine, mouse prostomach, and rat uterine bladder. Enhanced mitotic activity was observed in all the study tissues after exposure of both samples to TD given in some or in all (in the rat urinary bladder mucosal epithelium) doses.
    Gigiena i sanitariia 01/2011;
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    ABSTRACT: Long-term studies of 2 (cytohistology and genetic monitoring) laboratories of the Research Institute of Human Ecology and Environmental Hygiene, Russian Academy of Medical Sciences, to investigate the influence of environmental factors in the experiments made it possible to develop a noninvasive procedure for evaluating their cytological and cytogenetic impacts on man. A concurrent study of morphofunctional (histological, cytological) and cytogenetic parameters in both experimental and field trials can substantially extend and refine the interpretation of responses to environmental factors, which generates a need for using these parameters in hygienic and epidemiological studies.
    Gigiena i sanitariia 01/2007;
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    ABSTRACT: The assessment of the human risk of mutagens is a constituent of the general assessment of the risk of environment-pollutant chemicals to the population's health. An algorithm of assessing the risk of mutagens is proposed. Stage 1 (hazard identification) is to provide an expert analytical characterization of the mutagenic potential of the chemicals polluting the study object. Stage 2 (hazard characterization) is to analyze the quantitative dependences of the effect of mutagens in gametes and somatic cells of man and mammals. Stage 3 (effect evaluation) is to characterize the sources of pollution and the doses of mutagens affecting the population. Stage 4 (risk characterization) is to calculate the risk of mutagens to the population and individuals.
    Gigiena i sanitariia 01/2006;
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    ABSTRACT: The mutagenic effect of antituberculous drug dioxazid was studied on rats receiving this preparation in a dose of 25 mg/kg (in conversion to dioxidine) via inhalation route for 3 months. The percentage of cells with micronuclei and the content of polychromatophilic erythrocytes among all bone marrow erythrocytes and percentage of cells with micronuclei, protrusions, and binucleated cells in the lungs and urinary bladder were evaluated. Dioxazid caused no changes in organs, except the increase in the percentage of binucleated cells in bladder epithelium, which attests to minor cytotoxic effect of the drug for this organ.
    Bulletin of Experimental Biology and Medicine 12/2005; 140(5):532-4. · 0.34 Impact Factor
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    ABSTRACT: The authors propose to include not only a control of human populations, but also environmental factors (chemical, physical, and biological), and objects (to estimate the cumulative mutagenic activity of water, air, soil, and foodstuffs) in order to ensure human genetic safety. For evaluation of human cell genetic damage, they also propose to use the noninvasive multiple organ micronuclear test, by taking into account additional cardiological parameters. The results of this monitoring makes it possible to define higher genetic risk areas for warranting the necessity, priority, and nature of prophylactic measures and to identify a risk group of individuals to be thoroughly examined and to undergo health-promoting measures.
    Gigiena i sanitariia 01/2005;
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    ABSTRACT: Antibacterial preparation dioxidine administered four times in doses of 10, 100, and 300 mg/kg increased the incidence of micronucleated cells in the bone marrow, lungs, and large intestine of mice. Bone marrow cells were most sensitive, while cells of the lungs and large intestine exhibited lower sensitivity to the cytogenetic effect of dioxidine.
    Bulletin of Experimental Biology and Medicine 09/2004; 138(2):165-7. · 0.34 Impact Factor
  • L P Sycheva
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    ABSTRACT: In vivo cytogenetic effects of cyclophosphamide was simultaneously evaluated in 7 mouse organs. Cyclophosphamide produced most pronounced changes in the urinary bladder and bone marrow, the main target organs for this carcinogen. Mutagenic effects of the preparation were also detected in the lungs, large intestine, and stomach. This approach can be used for evaluation of organ specificity of mutagens and for prediction of the carcinogenic effects of chemical compounds.
    Bulletin of Experimental Biology and Medicine 05/2001; 131(4):374-6. · 0.34 Impact Factor
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    ABSTRACT: Three groups of women (aged 20-40 years) exposed to different levels of dioxins were studied in Chapaevsk town: 15 women working at the chemical fertilizer plant where occupational exposure to dioxins is possible; 16 women without dioxins occupational exposure, but living as far as 1-3 km from the plant; 14 women without dioxins occupational exposure and living as far as 5-8 km from the plant. No personal correlation related to dioxins exposure was found by chromosome aberrations (CA) in peripheral blood lymphocytes, micronuclei (MN) and nuclear anomalies in buccal mucosa cells. There were no significant differences between the groups in CA and MN. Karyopyknosis and karyorrhexis were significantly increased in the highest exposed group.
    Chemosphere 01/2001; 43(4-7):999-1004. · 3.14 Impact Factor
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    ABSTRACT: The paper presents the results of an integrated medical genetic survey carried out in the town of Chapayevsk. The survey included an estimation of the incidence of congenital malformations (CMF), congenital morphogenetic options (CMGO), evaluation of the frequency of chromosomal mutations of various types and micronuclei in human somatic cells. The incidence of CMF among newborn infants corresponds to that in Russia, but such forms of CMF as congenital hydrocephalus and agenesia and disgenesis of the kidney were more common in the town. The study ascertained that the average number of CMGO per child was on the increase. Cytogenetic findings unambiguously demonstrate that there was a spatial gradient within the town (from the plant to remote districts), higher rates of chromosomal aberrations. Further studies of the effects of dioxins on genetic health are required to assess the actual genetic risk due to its human contact.
    Gigiena i sanitariia 01/2001;
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    ABSTRACT: Cyclohexene exhibits no mutagenic effect in the Ames test. Products of its transformation (chlorination) produced a mutagenic effect in the Ames test on TA 100 strain without metabolic activation and in the micronucleus test on epithelial cells from mouse urinary bladder and colon. These findings are consistent with epidemiological data on higher incidence of urinary bladder and rectal cancers in subjects consuming chlorinated drinking water from surface water reservoirs.
    Bulletin of Experimental Biology and Medicine 07/2000; 129(6):581-3. · 0.34 Impact Factor
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    ABSTRACT: 1,2-Dimethylhydrazine (DMH) was administered to both genders of mice and rats by oral gavage for 3 days. Twenty-four hours later, an assessment of the incidence of micronucleated cells was made in the bone marrow and sections of the gastrointestinal tract. An increase in micronucleated cells was observed in the colon of both genders of both species of rodent. Negative responses were observed in the forestomach, stomach, duodenum, intestine of both species. The bone marrow micronucleus assays were essentially negative, but the absence of a precise definition of the MTD precludes a definitive conclusion from being drawn. These results are consistent with the selective carcinogenicity of DMH to the colon of the rodent GI-tract. DMH is also known to be carcinogenic to rat and mouse liver and, although it is known to induce micronuclei in the hepatocytes of rats, no such data exist for the mouse. Consequently, mice were administered DMH on 13 successive days, followed by 2/3 partial hepatectomy and assessment of micronucleated hepatocytes. A strong positive liver micronucleus assay response was observed. Thus, DMH selectively induces micronuclei in the colon and liver of rats and mice, consistent with its carcinogenicity to these two tissues. No qualitative differences between the genders was observed in any of the assays. These results indicate that the assessment of genetic toxicity in rodents should not rely solely on assays made in bone marrow.
    Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 07/1996; 368(2):115-20. · 3.90 Impact Factor
  • Gigiena i sanitariia 01/1994;
  • Gigiena i sanitariia 10/1993;
  • V S Zhurkov, L P Sycheva
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    ABSTRACT: The paper summarizes the results of several studies to modify the effects of the indirect-acting mutagen cyclophosphamide and the direct-acting fotrin in the presence of varying activity of the microsomal monooxygenase system. It also first shows how the effects of the mutagens are modified by inducing or inhibiting monooxygenase activity. A regression modification index is proposed to measure the level and direction of modification of mutagenic effects in experiments on mammals.
    Vestnik Rossiĭskoĭ akademii meditsinskikh nauk / Rossiĭskaia akademiia meditsinskikh nauk 02/1993;
  • O G Salamatova, L P Sycheva
    Gigiena i sanitariia 04/1992;
  • L P Sycheva, N P Burmantova, V S Zhurkov
    Gigiena i sanitariia 04/1989;
  • V S Zhurkov, L P Sycheva, N P Burmantova
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    ABSTRACT: Random bred male rats were given drinking water with phenobarbital (PB) (daily doses 0.4; 2 and 10 mg/kg) during 120 days. Activity of mixed function of oxygenases (content of cytochromes P-450) were enhanced after PB treatment in doses 2 and 10 mg/kg. Following PB treatment the animals were injected 5-times ethyleneimine derivate--fotrin at the doses 2, 4 and 7 mg/kg. The induction of mixed function of oxygenases resulted in significant decrease in the number of cells with chromosome aberrations.
    Biulleten' eksperimental'noĭ biologii i meditsiny 11/1988; 106(10):458-60.

Publication Stats

49 Citations
13.07 Total Impact Points

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Institutions

  • 2000–2011
    • Russian Academy of Medical Sciences
      • Laboratory of Mutagenesis
      Moskva, Moscow, Russia