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ABSTRACT: PURPOSE: Most nosocomial urinary tract infections are related to urinary catheters and their biofilm. For the research in this province, animal models are needed to explain the host-pathogen interactions and have an advantage in controlling external interference. This study investigated the validity of the denaturing gradient gel electrophoresis (DGGE) technique for identification of bacteria in a rat model of urinary catheter biofilm. METHODS: After cutting with aseptic technique, the 24-gauge polyurethane IV catheter was placed in the female rat bladder through the urethra. The catheters were kept in the bladder for 2, 4, or 6 weeks for each group. The genomic DNA was isolated from harvested biofilm of the extracted catheter, and DGGE was performed. The band patterns of DGGE results were analysed, and the sequences were compared using the BLAST from the NCBI. RESULTS: The results show that Pseudomonas aerusinosa, Escherichia coli, Enterococcus spp., and Corynebacterium sp. were the dominant bacterial species, regardless of the indwelling periods, and other species of bacteria, including Burkholderia and Achromobacter, were identified. The changes in bacterial distribution for the different indwelling periods were non-specific. CONCLUSIONS: This study using rat model of urinary catheter suggests that DGGE is a useful method in the analysis of the bacterial community in biofilms. Molecular techniques, including DGGE, are valuable to identify fastidious bacteria in the urinary catheter biofilm. This study may be used as fundamental data for studies involving human materials hereafter.
International Urology and Nephrology 04/2013; · 1.47 Impact Factor
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Dong Hoon Lee,
Seung Hwan Lee,
Koon Ho Rha,
In Young Choi, Ji Youl Lee,
Sae Woong Kim,
Sangchul Lee,
Sung Kyu Hong,
Seok-Soo Byun,
In Gab Jeong,
Jun Hyuk Hong,
Choung-Soo Kim,
Hwang Gyun Jeon,
Hyun Moo Lee,
Byung Ha Chung
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ABSTRACT: The purpose of this article was to announce the establishment of the multicenter Korean Prostate Cancer Database (K-CaP) and to provide urologists with details about K-CaP's methodology.
The initial participating K-CaP institutions include five medical centers in Korea. First, we registered prostate cancer patients who underwent radical prostatectomy as the basic background data. K-CaP is poised to combine these initial observational longitudinal studies with those of other eligible institutions as the database grows. All current prostate cancer patients in Korea are able to be registered into the Web-based database system and thereby have a role in several observational studies. The structure of the database for K-CaP was developed by matching it with the respective data from different studies. The operability of the K-CaP database system was verified by using the existing databases from three participating institutions.
The analysis of clinicopathologic characteristics of patients with the use of the Web-based database was successfully conducted. We confirmed the accurate operation of the Web-based database system without any difficulties.
We are announcing the establishment of K-CaP the first database of comprehensive observational longitudinal studies about prostate cancer in Korea. The database will be successfully maintained by sufficiently and continuously updating all patient data covering several treatments. Complete statistical results for registered prostate cancer patients are forthcoming for the basic background data to establish the database. Even though much trial and error are expected during the development process, we expect that K-CaP will eventually become one of the most powerful longitudinal observation databases.
Korean journal of urology 04/2013; 54(4):229-33.
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ABSTRACT: Stress urinary incontinence (SUI) is an embarrassing problem affecting a large number of women and interfering with their quality of life. The injury or weakness of urethral supporting tissues by childbirth and aging has been considered as key factors in the development of the SUI. In this study, plasmid DNA (pDNA; encoding for bFGF) complex-loaded poly(dl-lactic-co-glycolic acid) (PLGA)/Pluronic F127 mixture dispersed with polycaprolactone (PCL) microspheres was prepared as an injectable bioactive bulking agent that may provide bulking effect (by PCL microspheres) and allow stimulation of the defect tissues around urethra (by synthesis of bFGF from cells or tissues transfected by the pDNA complex) for the effective treatment of SUI. From in vitro experiments, the pDNA complex incorporated in the bulking agent was released in a sustained manner over 84 days (≥80% of the initial loading amount). The pDNA complex was effectively transfected into fibroblasts and the cells were continuously producing the target protein, bFGF. From the in vivo study using hairless mice and Sprague-Dawley rats, it was confirmed that the pDNA complex released from the bulking agent is transfected into surrounding cells/tissue, and the cells/tissues synthesize sufficient bFGF to regenerate smooth muscle with biological function around the urethra. Basis on these results, the pDNA (encoding for bFGF) complex-loaded PLGA/Pluronic F127 mixture dispersed with PCL microspheres can be a promising bioactive bulking agent system for the fundamental cure of SUI.
Biomaterials 03/2013; · 7.40 Impact Factor
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ABSTRACT: OBJECTIVE: To prevent cavernous nerve injury and corpus cavernosum apoptosis-induced erectile dysfunction (ED) after prostatectomy surgery, we investigated whether oral administration of udenafil combination with covering adipose-derived stem cells (ADSCs) and brain-derived neurotrophic factor (BDNF) immobilized poly-lactic-co-glycolic (PLGA) membrane on the injured cavernous nerve could further improve erectile dysfunction. METHODS: Adult Sprague-Dawley rats were divided into 5 groups: normal group (sham-operated group), bilateral cavernous nerve injury (BCNI) group (BCNI group), udenafil group (oral administration of udenafil 20 mg/kg daily), AB group (BCNI group with ADSCs covered with BDNF membrane on cavernous nerve), AB/udenafil group (AB group with udenafil group). After 4 weeks, erectile function was examined before tissue harvest. Penile tissues were evaluated in terms of the expression of smooth muscle actin (SMA), neuronal nitric oxide synthase (nNOS), and vascular endothelial growth factor (VEGF). The cyclic guanosine monophosphate (cGMP) level of the corpus cavernosum was quantified by cGMP assay. RESULTS: AB/udenafil treatment markedly improved erectile function and prevented the architecture damage of the corpus cavernosum, compared with other treated groups. Udenafil had no statistical significance on increasing nNOS expression, but enhanced VEGF expression. On the contrary, the AB group had no statistical significance on enhancing VEGF expression, but increased nNOS expression. AB/udenafil treatment significantly increased nNOS expression, VEGF expression, and elevated cGMP level, compared with the udenafil group and AB group. CONCLUSION: The orally administered udenafil combination with ADSC/BDNF-membrane system protected cavernous nerve and improved angiogenesis in the corpus cavernosum, which further maintained erectile function in a rat model of postprostatectomy erectile dysfunction.
Urology 03/2013; · 2.43 Impact Factor
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ABSTRACT: To investigate the type of nocturia and concomitant voiding dysfunction (VD) and the effect of desmopressin treatment on nocturia in women.
We reviewed 84 women who experienced more than 2 nocturia episodes as recorded on a pretreatment frequency volume chart and who were treated with desmopressin. All patients underwent history taking, physical examination, urinalysis, International Prostate Symptom Score assessment, completion of a urinary sensation scale, and completion of a 3 day frequency volume chart. Nocturia was divided into nocturnal polyuria (NP), reduced nocturnal bladder capacity (RNBC), and mixed type. After treatment with desmopressin, a reduction in nocturia of over 50% compared with baseline was regarded as effective.
Among 84 women, the most common concomitant VD was overactive bladder (OAB, 60.7%). NP was observed in 70.2% (59/84) of the women, RNBC in 7.1% (6/84), and mixed type in 22.6% (19/84). After medication with desmopressin, 73 women (86.9%) showed a significantly reduced number of nocturia episodes (1.4±1.5) compared with baseline (3.7±1.3, p<0.05). Eleven women (13.1%) did not show improvement. Of the 73 women who showed improvement, 41 women showed a reduction of more than 50% over baseline, and these women had a lower baseline urgency grade.
In the majority of women, nocturia coexisted with other VD such as OAB. Treatment with desmopressin effectively reduced the nocturia. However, other lower urinary tract symptoms (LUTS) such as urgency may reduce the effect of desmopressin. Therefore, consideration of concomitant LUTS seems to be necessary to increase the treatment effect of desmopressin on nocturia in women.
Korean journal of urology 02/2013; 54(2):95-9.
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ABSTRACT: To investigate the differences in the histopathologic and functional characteristics of 3 rat models of stress urinary incontinence.
A total of 24 female, 10-week-old, Sprague-Dawley rats were randomly divided into 4 groups: normal, electrocauterization, pudendal denervation, and vaginal distension. At 2 weeks after surgery, the leak point pressure was measured to detect urinary leakage. Urethral tissue samples were collected for histological examination.
The smooth muscle content in the electrocauterization group was significantly decreased compared with that in all other groups, indicating that electrocauterization caused the most severe injury. A blood vessel marker, von Willebrand factor, was co-stained with α-smooth muscle actin to detect the blood vessel distribution. No significant differences were seen in von Willebrand factor expression among the 4 groups, other than in the electrocauterization group, in which we could hardly observe blood vessel expression. Protein gene product 9.5 staining was used to detect nerve fibers and cells. Protein gene product 9.5 expression was significantly lower in all the treatment groups compared with that in the normal group (P <.05), in particular, in the electrocauterization and pudendal denervation groups (P <.01). The leak point pressure was significantly lower in the electrocauterization (P <.01), pudendal denervation (P <.01), and vaginal distension (P <.05) groups than in the normal group.
The vaginal distension model should mainly be used as the myogenic damage stress urinary incontinence animal model; the pudendal denervation model mainly as the neurogenic damage stress urinary incontinence animal model; and the electrocauterization model as the vasculogenic, neurogenic, and myogenic damage animal model.
Urology 02/2013; 81(2):465.e1-6. · 2.43 Impact Factor
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Woong Jin Bae,
Jang Ho Bae,
Sae Woong Kim,
Byung Ha Chung,
Jang Hwan Kim,
Choung Soo Kim,
Hyun Moo Lee,
Kyung Seop Lee,
Tag Keun Yoo,
Sun Il Kim,
Seok Soo Byun, Ji Youl Lee
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ABSTRACT: PURPOSE: Alpha-blockers improve lower urinary tract symptoms (LUTS) associated with benign prostatic obstruction (BPO). Nocturia-a storage symptom-is a common complaint in men, however, it does not fully respond to alpha-blocker therapy, likely due to its multifactorial pathophysiology. We evaluated the efficacy and safety of desmopressin as add-on therapy for refractory nocturia in men previously treated with an alpha-blocker for LUTS. MATERIALS AND METHODS: Eligible patients were men 50 years of age or older with LUTS and persistent nocturia despite alpha-blocker treatment for a minimum of 4 weeks. The optimum dose of oral desmopressin was determined during a 4-week dose-titration period, and this dose was maintained for 24 weeks. Flow volume charts, total IPSS, IPSS subscores, uroflowmetry and post-void residual urine volume were assessed. RESULTS AND CONCLUSIONS: A total of 216 patients were enrolled in the study. Of these patients, there were 158(76%) patients with nocturnal polyuria, 15(7.2%) with decreased nocturnal bladder capacity, and 35(16.8%) with nocturia due to both causes. The number of nocturnal voids significantly decreased from a baseline mean of 7.0 to 5.7 episodes for 3 days at the 24 week visit. The average IPSS total and IPSS subscore significantly decreased by 4 weeks and were maintained at 24 weeks. In patients aged less than 65 years, IPSS voiding subscores were significantly improved at 24 weeks compared to those 65 years or over. Desmopressin add-on therapy for refractory nocturia in men previously treated with an alpha-blocker for LUTS improved voiding symptoms as well as nocturia, storage symptoms.
The Journal of urology 01/2013; · 4.02 Impact Factor
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ABSTRACT: PURPOSE: To investigate the effect of the ethanol on corpus cavernosum of the type 2 diabetes mellitus (type 2 DM) rat, since previous studies have reported the positive effect of moderate drinking on type 2 DM and endothelial function. METHODS: Otsuka Long Evans Tokushima Fatty (OLETF, n = 28) and Long Evans Tokushima Otsuka (LETO, n = 28) male rats were assigned to four groups: control (LETO, n = 14), control/ethanol (LETO with ethanol, n = 14), fatty (OLETF, n = 14) and fatty/ethanol (OLETF with ethanol, n = 14). The control/ethanol and fatty/ethanol groups were fed Lieber-DeCarli Regular EtOH. After 10 weeks, an intraperitoneal glucose tolerance test (IPGTT) was performed. RT-PCR and Western blot analyses of the RhoA/Rho kinase in the corpus cavernosum were performed. The expression of endothelial nitric oxide synthase (eNOS) and neuronal nitric oxide synthase (nNOS) in the corpus cavernosum was evaluated by immunohistochemistry. RESULTS: After a 10-week ethanol intake, the body weight and the IPGTT of the fatty/ethanol group were significantly lower compared with the fatty group (p < 0.05). A significantly increased mRNA and protein expression in the fatty group was observed compared with the control group by RT-PCR and Western blot analyses (p < 0.05): A significantly decreased expression was observed in the fatty/ethanol group compared with the fatty group (p < 0.05). The significantly increased expression of eNOS was observed in the fatty/ethanol group compared with the fatty group by immunohistochemistry. CONCLUSIONS: Long-term ethanol intake may preserve the erectile function in type 2 DM through the down-regulation of the RhoA/Rho kinase pathway.
International Urology and Nephrology 01/2013; · 1.47 Impact Factor
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Woong Jin Bae,
Sun Gook Ahn,
Jun Ho Bang,
Jang Ho Bae,
Yong Sun Choi,
Su Jin Kim,
Hyuk Jin Cho,
Sung Hoo Hong, Ji Youl Lee,
Tae-Kon Hwang,
Sae Woong Kim
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ABSTRACT: To assess the risk factors for developing urinary retention after removal of the urethral catheter on postoperative day 1 in benign prostatic hyperplasia patients who underwent Greenlight HPS laser photoselective vaporization prostatectomy (PVP).
The study included 427 men who underwent Greenlight HPS laser PVP between 2009 and 2012, excluding patients in whom a catheter was maintained for more than 1 day because of urethral procedures. In all patients, a voiding trial was performed on postoperative day 1; if patients were unable to urinate, the urethral catheter was replaced before hospital discharge. The patients were divided into two groups: early catheter removal (postoperative day 1) and late catheter removal (urethral catheter reinsertion). Preoperative and perioperative parameters were compared between the groups.
Catheters were successfully removed in 378 (88.6%) patients on postoperative day 1. In 49 patients, the catheters were reinserted and removed a mean of 6.45±0.39 days after surgery. In a multivariate analysis, a history of diabetes was the most significant predictor (p=0.028) of failure of early catheter removal, followed by operative time (p=0.039). There were no significant differences in age, prostate volume, International Prostate Symptom Score, or urodynamic parameters between the two groups.
It is feasible, safe, and cost-effective to remove the urethral catheter on postoperative day 1 after Greenlight HPS laser PVP, but the procedure should be done carefully in patients who have history of diabetes or an extended operative time.
Korean journal of urology 01/2013; 54(1):31-5.
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ABSTRACT: Objective: To compare the clinical outcomes of two-port laparoscopic donor nephrectomy (TPLDN) versus hand-assisted laparoscopic donor nephrectomy (HALDN). Patients and Methods: Between November 2010 and March 2012, 100 kidney donors scheduled for left nephrectomy were alternatively assigned to HALDN or TPLDN in a 1:1 fashion. All procedures were performed by the same laparoscopic surgeon. Demographic data and intraoperative and early postoperative data were collected prospectively and analyzed. Results: There was no difference in the operating time (133±12 vs 142±17 minutes, p=0.07), blood loss (55±46 vs 58±52 mL, p=0.84), complication rate (10 vs 12%, p=0.74), and length of hospital stay (3.8±0.8 vs 4.1±2.8 days, p=0.5) between the HALDN and TPLDN groups. The warm ischemia time was longer in the TPLDN group (2.2±0.7 vs 3.5±0.9 minutes, p<0.001). No statistically significant differences were found in the analgesic requirement and the visual analog pain scores. There was a trend toward quicker return to 100% recovery in the TPLDN group (60±46 vs 39±15 days, p=0.05). The TPLDN group had a significantly smaller surgical incision (8.2±0.6 vs 5.5±0.4 cm, p<0.001) and higher scar satisfaction score (7.8±1.5 vs 8.6±1.3, p=0.02) than the HALDN group. No differences were found in the recipient serum creatinine values or in the incidence of delayed graft function. Conclusions: In comparing TPLDN and HALDN, there was no significant difference in a majority of the operative and postoperative parameters. TPLDN might be associated with smaller surgical incision, improved cosmetic satisfaction, and equivalent recipient graft function.
Journal of endourology / Endourological Society 12/2012; · 1.75 Impact Factor
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ABSTRACT: Background/Aims: Lymphedema is a clinically incurable disease that occurs commonly after lymph node dissection and/or irradiation. Several studies have recently demonstrated that extracorporeal shock wave therapy (ESWT) could promote lymphangiogenesis associated with expression of vascular endothelial growth factor (VEGF)-C. This research concerned primarily the synergistic effect of ESWT combined with VEGF-C incorporated hydrogel (VEGF-C hydrogel) combination therapy for promoting lymphangiogenesis and ultimately alleviating lymphedema. Methods: The VEGF-C hydrogel was applied to the injury site in a mouse model of lymphedema and then regularly underwent ESWT (0.05 mJ/mm(2), 500 shots) every 3 days for 4 weeks. Results: Four weeks after the treatment, mice treated with VEGF-C hydrogel and ESWT showed signs of the greatest decrease in edema/collagenous deposits when compared with the other experimental group. LYVE-1-positive vessels also revealed that the VEGF-C/ESWT group had significantly induced the growth of new lymphatic vessels compared to the other groups. Western blot analysis showed that expression of VEGF-C (1.24-fold) and VEGF receptor-3 (1.41-fold) was significantly increased in the VEGF-C/ESWT group compared to the normal group. Conclusion: These results suggested that VEGF-C and ESWT had a synergistic effect and were very effective in alleviating the symptoms of lymphedema and promoting lymphangiogenesis.
Journal of Vascular Research 11/2012; 50(2):124-133. · 2.65 Impact Factor
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ABSTRACT: Zinc is one of the trace minerals in the body and is known to have an anticancer effect by inducing apoptosis in prostate cancer. We aimed to investigate the antiproliferative effects of a zinc-citrate compound in bladder cancer.
A bladder cancer cell line (MBT-2) was treated with a zinc-citrate compound at different time intervals and concentrations. Mitochondrial (m)-aconitase activity was determined by use of the aconitase assay. DNA laddering analysis was performed to investigate apoptosis of MBT-2 cells. The molecular mechanism of apoptosis was investigated by Western blot analysis of p53, p21(waf1), Bcl-2, Bcl-xL, and Bax and also by caspase-3 activity analysis.
Treatment with the zinc-citrate compound resulted in a time- and dose-dependent decrease in cell number of MBT-2 cells. M-aconitase activity was significantly decreased. DNA laddering analysis indicated apoptosis of MBT-2 cells. The zinc-citrate compound increased the expression of p21(waf1) and p53 and reduced the expression of Bcl-2 and Bcl-xL proteins but induced expression of Bax protein. The zinc-citrate compound induced apoptosis of MBT-2 cells by activation of the caspase-3 pathway.
We have shown that a zinc-citrate compound induces apoptotic cell death in a bladder cancer cell line, MBT-2, by caspase-3 activation through up-regulation of apoptotic proteins and down-regulation of antiapoptotic proteins.
Korean journal of urology 11/2012; 53(11):800-6.
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Su Jin Kim,
Sae Woong Choi,
Kyung Jae Hur,
Sang Hoon Park,
Young Chul Sung,
Y-Shin Ha,
Hyuk Jin Cho,
Sung-Hoo Hong, Ji Youl Lee,
Tae-Kon Hwang,
Sae Woong Kim
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ABSTRACT: To evaluate the combined role of mescenchymal stem cells (MSCs) infected with recombinant adenoviruses expressing human BDNF (rAd/hBDNF) on the erectile dysfunction in rat with cavernous nerve injury.
Rats divided into 4 groups: control group, bilateral cavernous nerve crushing group (BCNC group), BCNC with MSCs group and BCNC with MSCs infected with rAd/hBDNF group. After 4-week, functional assessment was done. PKH26 and BDNF staining of major pelvic ganglion and masson's trichrome staining of corpus cavernosum were performed. Western blot analysis of endothelial nitric oxide synthase (eNOS) and neuronal nitric oxide synthase (nNOS) was done in corpus cavernosum.
After 4 weeks, BCNC with MSCs and MSCs infected with rAd/hBDNF groups showed significantly well-preserved erectile function compared with BCNC group. Moreover, the erectile function of MSCs infected with rAd/hBDNF group was significantly well-preserved than BCNC with MSCs group. The smooth muscle of corpus cavernosum was significantly preserved in BCNC with MSCs and MSCs infected with rAd/hBDNF groups compared with BCNC group. More preservation of smooth muscle was observed in rats with MSCs infected with rAd/hBDNF than with MSCs alone. Significant increase expression of eNOS and nNOS was noted in rats with MSCs infected with rAd/hBDNF than with MSCs alone.
The erectile function was more preserved after injection with MSCs infected with rAd/hBDNF in rat with ED caused by cavernous nerve injury. Therefore, the use of MSC infected with rAd/hBDNF may have a better treatment effect on ED cause by cavernous nerve injury.
Korean journal of urology 10/2012; 53(10):726-32.
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ABSTRACT: OBJECTIVE: To present our experience of 2-port laparoscopic donor nephrectomy (TPLDN) and to introduce a simple retraction suture technique. MATERIAL AND METHODS: From October 2010 to November 2010, 10 consecutive, left TPLDNs were performed. The SILS Port was inserted through the umbilicus and was used for 5-mm laparoscope and laparoscopic instruments. A 12-mm trocar, for another working channel, was inserted along the midclavicular line at the umbilicus level. No articulating or bent laparoscopic instruments were needed. Two retraction sutures were used; a 3-0 Prolene suture was passed through the pericolic fat and then brought out of the abdominal wall and secured to the skin. Another suture was passed around the lower pole fat of the kidney, which was then fixed on the abdominal wall with a clamp. RESULTS: TPLDN was performed successfully in all patients, with no conversions to a conventional laparoscopic procedure or additional trocar insertion needed. The mean operative time was 152 ± 16.3 minutes, and the mean warm ischemia time was 2.9 ± 0.9. The average estimated blood loss was 77.3 ± 70 mL, and the postoperative hospital stay was 3.6 ± 0.6 days. No perioperative or postoperative complications were observed. Cosmesis was excellent, with a mean incision length of 5.6 ± 0.4 cm. All allografts functioned after transplantation. CONCLUSION: Our initial experience suggests that TPLDN appears safe and cosmetically effective and a feasible option for donor nephrectomy. The appropriate use of retraction sutures, during TPLDN, helps to overcome the technical problems associated with the inadequate retraction and exposure.
Urology 09/2012; · 2.43 Impact Factor
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ABSTRACT: To compare the safety and efficacy of multiple-tract percutaneous nephrolithotomy (PCNL) with single-tract PCNL for complex renal stones.
A total of 109 consecutive PCNL procedures for unilateral complex renal calculi (staghorn or complex caliceal calculi) were performed at our institution. Thirty patients received multiple-tract PCNL and 79 patients underwent single-tract PCNL. The two groups had comparable demographic data except for a smaller stone burden and fewer complete staghorn calculi in those undergoing single-tract PCNL. Variables of interest included operative time, blood loss, change of serum creatinine, transfusion rates, length of hospital stay, stone clearance, number of ancillary procedures, and complication rates.
The number of tracts used for multiple-tract PCNL was two tracts in 20 patients, three tracts in 9, and four tracts in 1. Significant differences were not observed when the single-tract PCNL results were compared with the multiple-tract PCNL in terms of success rate, operative time, transfusion rate, drop in hemoglobin, hospitalization time, complication rate, and rise in serum creatinine. The need for ancillary procedures was more common in patients undergoing multiple-tract PCNL (53.3% versus 24.1%; p = 0.003). No long-term sequela were noted during the median follow up of 24 months in any patient.
The results of the present study show that multi-tract PCNL for appropriately chosen stones/patients has similar safety and effectiveness as single PCNL in patients with smaller and less complex stones.
The Canadian Journal of Urology 08/2012; 19(4):6360-5. · 0.64 Impact Factor
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ABSTRACT: Postprostatectomy erectile dysfunction (ED) is the major problem for patients with clinically localized prostate cancer. Recently, gene and stem cell-based therapy of the corpus cavernosum has been attempted for postprostatectomy ED, but those therapies are limited by rapid blood flow and disruption of the normal architecture of the corpus cavernosum. In this study, we attempted to regenerate the damaged cavernous nerve (CN), which is the main cause of ED. We investigated the effectiveness of human adipose-derived stem cell (hADSC) and nerve growth factor-incorporated hyaluronic acid-based hydrogel (NGF-hydrogel) application on the CN in a rat model of bilateral cavernous nerve crush injury. Four weeks after the operation, erectile function was assessed by detecting the intracavernous pressure (ICP)/arterial pressure level by CN electrostimulation. The ICP was significantly increased by application of hADSC with NGF-hydrogel compared to the other experimental groups. CN and penile tissue were collected for histological examination. PKH-26 labeled hADSC colocalized with beta III tubulin were shown in CN tissue sections. hADSC/NGF-hydrogel treatment prevented smooth muscle atrophy in the corpus cavernosum. In addition, the hADSC/NGF-hydrogel group showed increased endothelial nitric oxide synthase protein expression. This study suggests that application of hADSCs with NGF-hydrogel on the CN might be a promising treatment for postprostatectomy ED.
Tissue Engineering Part A 07/2012; · 4.64 Impact Factor
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ABSTRACT: Abstract The retraction of the liver is essential to obtain a better laparoscopic view in right nephrectomy. A simple technique for liver retraction during laparoscopic right renal surgery using surgical cotton is presented. The advantage of the presented method is that this technique is simple, does not require special equipments, and eliminates the need for any additional ports.
Journal of endourology / Endourological Society 07/2012; · 1.75 Impact Factor
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ABSTRACT: To investigate the effect of human muscle-derived stem cells (hMDSCs) on ameliorating impaired detrusor contractility in a cryoinjured bladder murine model.
The hMDSCs were isolated and cultured by modified preplate technique, and only CD34-positive hMDSCs were extracted by Mini-MACS kits. Isolated hMDSCs were prelabeled with PKH26 and injected into the cryoinjured bladder to observe the pattern and characteristics. The nude mice were subdivided into three groups: normal group (N), cryoinjury bladder group with saline injection (C), and hMDSCs injection group after cryoinjury (M). At 2 weeks after injecting hMDSCs, we compared the contractility of bladder muscle strip stimulated by electrical field stimulation (EFS), acetylcholine (Ach.), and adenosine triphosphate (ATP), and the bladder smooth muscle tissue was examined by immunohistochemistry.
The contractile powers of bladder muscle strip in the C group were more decreased than the N group after EFS, Ach, and ATP treatment (P < .05). The bladder contractility of the M group was more increased than in the C group (P < .05), but was lower than the N group after EFS and Ach treatment. However, there was no significant difference of contractile power between the C and M groups after ATP stimulation. In immunohistochemical staining, the thickness of the bladder smooth muscle layer in the M group was significantly increased compared with the C group, and PKH26-labeled implanted cells were positive for smooth muscle cell differentiation marker (α-SMA) in the injected region.
hMDSCs injection increased cholinergic bladder contractile power but not the purinergic component of bladder contraction after cryoinjury.
Urology 07/2012; 80(1):224.e7-11. · 2.43 Impact Factor
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ABSTRACT: To evaluate the efficacy of photoselective vaporization of the prostate (PVP) with the 120 W Greenlight high performance system (HPS) laser for the treatment of benign prostatic hyperplasia (BPH) with intravesical prostatic protrusion (IPP).
This study was conducted on 389 BPH patients who underwent PVP with the 120 W HPS laser from April 2009 to August 2011. The patients were divided into groups according to IPP: group I was defined as IPP of 0 to 5 mm (n=216), group II as IPP of 5 to 10 mm (n=135), and group III as IPP above 10 mm (n=38). Prostate volume, prostate-specific antigen, International Prostate Symptom Score (IPSS), maximal urinary flow rate (Qmax), and postvoid residual volume (PVR) were assessed and checked at postoperative 1, 3, 6, and 12 months.
There was a significant difference in the mean prostate size in each group (p<0.05). The preoperative total IPSS score, IPSS voiding symptom score, and quality of life score were not significantly different. However, the IPSS storage symptom score was significantly different between groups 1 and 2 and group 3. IPSS scores, Qmax, and PVR at postoperative 1, 3, 6, and 12 months showed significant improvement compared with preoperative values.
The degree of IPP can affect storage symptoms. However, there is no significant correlation between the degree of IPP and postoperative results. Also, the degree of IPP does not affect short- and long-term PVP results. Proper elimination of bladder outlet obstruction is important for symptomatic relief.
Korean journal of urology 07/2012; 53(7):472-7.
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ABSTRACT: To investigate the antiproliferative effects of zinc-citrate compound on hormone refractory prostate cancer (HRPC).
HRPC cell line (DU145) and normal prostate cell line (RWPE-1) were treated with zinc, citrate and zinc-citrate compound at different time intervals and concentrations to investigate the effect of zinc-citrate compound. Mitochondrial (m)-aconitase activity was determined using aconitase assay. DNA laddering analysis was performed to investigate apoptosis of DU145 cells. Molecular mechanism of apoptosis was investigated by Western blot analysis of P53, P21(waf1), Bcl-2, Bcl-xL and Bax, and also caspase-3 activity analysis.
Treatment with zinc-citrate compound resulted in a time- and dose-dependent decrease in cell number of DU145 cells in comparison with RWPE-1. M-aconitase activity was significantly decreased. DNA laddering analysis indicated apoptosis of DU145 cells. Zinc-citrate compound increased the expression of P21(waf1) and P53, and reduced the expression of Bcl-2 and Bcl-xL proteins but induced the expression of Bax protein. Zinc-citrate compound induced apoptosis of DU145 cells by activation of the caspase-3 pathway.
Zinc-citrate compound can induce apoptotic cell death in DU145, by caspase-3 activation through up-regulation of apoptotic proteins and down-regulation of antiapoptotic proteins.
Chinese Journal of Cancer Research 06/2012; 24(2):124-9. · 0.18 Impact Factor
