Publications (216)563.72 Total impact
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Dataset: Distribution of reef building corals at some locations of the Red Sea, Egypt
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Dataset: Assessment of present status and future needs
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Dataset: ACP-EU Proceedings
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Dataset: Application of cell culture for coral reef eclogy
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Dataset: A new innovated
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Dataset: A quantitative ecological assessment
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Dataset: Corals differential susceptibilities to bleaching
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Dataset: A coral damage index
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Dataset: Coral population at Gasus 2 and El-Fanadir
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Dataset: Distribution and space monopolization
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Dataset: Coral reef restoration and artificial reef management
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Dataset: Effect of physico-chemical
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Dataset: Coral reef rehabilitation off Hurghada, Egypt, Red Sea- Preliminary results
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Dataset: Coral reef rehabilitation, Technical Options and Necessary Political and Socioeconomic Frame
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Dataset: Coral diversity indices
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Dataset: Community structure and diversity of reef building corals at Sharm El-Sheikh
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Dataset: An alarming threat
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Dataset: Cell culture from two Red Sea benthic invertebrates
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Dataset: Cultivation of primmorphs
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Article: Development of a morphogenetically active scaffold for three-dimensional growth of bone cells: biosilica-alginate hydrogel for SaOS-2 cell cultivation.
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ABSTRACT: Polymeric silica is formed from ortho-silicate during a sol-gel formation process, while biosilica is the product of an enzymatically driven bio-polycondensation reaction. Both polymers have recently been described as a template that induces an increased expression of the genes encoding bone morphogenetic protein 2 (BMP-2) and osteoprotegerin in osteoblast-related SaOS-2 cells; simultaneously or subsequently the cells respond with enhanced hydroxyapatite formation. In order to assess whether the biocompatible polymeric silica/biosilica can serve as a morphogenetically active matrix suitable for three-dimensional (3D) cell growth, or even for 3D cell bioprinting, SaOS-2 cells were embedded into a Na-alginate-based hydrogel. Four different gelatinous hydrogel matrices were used for suspending SaOS-2 cells: (a) the hydrogel alone; (b) the hydrogel with 400 μm ortho-silicate; (c) the hydrogel supplemented with 400 μm ortho-silicate and recombinant silicatein to allow biosilica synthesis to occur; and (d) the hydrogel with ortho-silicate and BSA. The SaOS-2 cells showed an increased growth if silica/biosilica components were present in the hydrogel. Likewise intensified was the formation of hydroxyapatite nodules in the silica-containing hydrogels. After an incubation period of 2 weeks, cells present in silica-containing hydrogels showed a significantly higher expression of the genes encoding the cytokine BMP-2, the major fibrillar structural protein collagen 1 and likewise of carbonic anhydrase. It is concluded that silica, and to a larger extent biosilica, retains its morphogenetic/osteogenic potential after addition to Na-alginate-based hydrogels. This property might qualify silica hydrogels to be also used as a matrix for 3D cell printing. Copyright © 2013 John Wiley & Sons, Ltd.Journal of Tissue Engineering and Regenerative Medicine 04/2013; · 3.28 Impact Factor
Top co-authors
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Institutions
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1987–2013
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Johannes Gutenberg-Universität Mainz
- • Institute of Physical Chemistry
- • Institut für Physiologische Chemie
Mainz, Rhineland-Palatinate, Germany
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2011
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University of Ibadan
- Department of Chemistry
Ibadan, Oyo State, Nigeria
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2006–2007
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Università degli Studi di Napoli Federico II
- Department of Chemistry of Natural Substances
Napoli, Campania, Italy -
Freie Universität Berlin
Berlin, Land Berlin, Germany
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2005
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Institute Of Physical Materials Science, Siberian Branch of the Russian Academy Of Sciences
Ulan-Ude, Respublika Buryatiya, Russia -
Institut für Chemie
Rostock, Mecklenburg-Vorpommern, Germany
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2002
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National Academy of Sciences of Ukraine
Kiev, Misto Kyyiv, Ukraine
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2000
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The University of Tokyo
Kashiwa, Chiba-ken, Japan
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1994
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University of Zagreb
Varaždin, Varazdinska Zupanija, Croatia
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