Publications (20)28.14 Total impact
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Article: Synthesis of a novel organic nonlinear optical chromophore and the Testing for µgβ Value
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ABSTRACT: A novel second-order nonlinear optical chromophore (DCDHF-2-V) was synthesized from 3-Hydroxy-3-methyl-2-butanae, propanedinitrile and 4-diethylaminobenzaldehyde by aldol condensation reaction. Molecular structural characterization was investigated by elemental analysis, FTIR, and 1H-NMR spectra. The melting point obtained from DSC thermogram was almost 251 °C, and the compound shows a thermal stability up to 270 °C. Second-order NLO properties of the chromophore were measured by solvatochromic method. From the quantum-mechanical two-level model, it can be obtained that the µg β value of DCDHF-2-V is 6520 × 10−48esu at the wavelength of 1064 nm.Optoelectronics Letters 04/2012; 3(1):50-53. -
Article: Application of response surface methodology to optimise ultrasonic-assisted extraction of four chromones in Radix Saposhnikoviae.
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ABSTRACT: Radix Saposhnikoviae is one of the most famous Chinese herbal medicines with many pharmacological activities towards inflammatory symptoms and antioxidation. Chromones are considered as one of the effective components. It is important to find a reasonable method to extract the chromones in S. divaricata. To develop an ultrasonic-assisted extraction (UAE) to extract chromones in Radix Saposhnikoviae and to optimise extraction conditions. Four chromones (prim-O-glucosylcimifugin, cimifugin, 5-O-methylvisammioside and sec-O-glucosylhamaudol) were extracted by the UAE method combined with response surface methodology (RSM). Box-Behnken design (BBD) was applied to evaluate the effects of three independent variables (ethanol concentration, extraction time and extraction temperature) on the chromones yield of Radix Saposhnikoviae. Correlation analysis of the mathematical-regression model indicated that a quadratic polynomial model could be employed to optimise the extraction of chromones by UAE method. The optimal conditions to obtain the highest chromones yield of Radix Saposhnikoviae were a solvent of 75% ethanol, an extraction time of 48 min and an extraction temperature of 67°C. Under these optimal conditions, the experimental values agreed closely with the predicted values. The analysis of variance indicated a high goodness of model fit and the success of RSM method for optimising chromones extraction in Radix Saposhnikoviae.Phytochemical Analysis 03/2011; 22(4):313-21. · 2.63 Impact Factor -
Article: Optimization of ginsenosides hydrolyzing beta-glucosidase production from Aspergillus niger using response surface methodology.
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ABSTRACT: To optimize ginsenosides hydrolyzing beta-glucosidase production from Aspergillus niger, response surface methodology was carried out in two stages. The Plackett-Burman design was achieved to screen the important variables that influence beta-glucosidase production. Among 10 variables (wheat bran, soybean powder, CaCl(2), ginsenosides, KH(2)PO(4), MgSO(4), polyethylene glycol (PEG), medium volume, inoculum size, and stirring speed), it was found that wheat bran, KH(2)PO(4), and stirring speed had significant effect on beta-glucosidase activity due to very low p-values (p<0.05). Subsequently, wheat bran, KH(2)PO(4), and stirring speed were further optimized using central composite design. The optimal beta-glucosidase production was predicted to be 4650.14 U/ml with the combination of factors (wheat bran, 34.51 g/l; KH(2)PO(4), 1.78 g/l; stirring speed, 161.60 rpm/min). Finally, under optimal fermentation conditions, ginsenoside Rb(1) was converted to Rd and F(2) by A. niger within 10 min. Little compound K was detected at 30 min, and finally F(2) was completely transformed to compound K within 8 h. The putative conversion pathway of Rb(1) by A. niger was Rb(1), Rd, F(2), and compound K.Biological & Pharmaceutical Bulletin 11/2008; 31(10):1870-4. · 1.66 Impact Factor -
Article: [Effects of zingerone on fat storage in ovariectomized rats].
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ABSTRACT: We reported that ginger prevented obesity in mice fed a high-fat diet in previous study. In this experiment, we examined the effects of zingerone, the major pungent component of ginger on fat storage in ovariectomized (Ovx) rats. Oral administration of 170 mg/kg zingerone significantly reduced body weight and the final parametrail adipose tissue weight in ovariectomized rats. Blood glucose levels after oral administration of glucose were lower in zingerone-treated Ovx-rats than in the Ovx-rats (control). Basal lipolysis in zingerone-treated Ovx-rats was increased compared with that in the Ovx-rats. Zingerone significantly increased norepinephrine-induced lipolysis associated with the translocation of hormone-sensitive lipase (HSL) from the cytosol to lipid droplets in adipocytes. These results indicate that zingerone may prevent the fat storage through increasing norepinephrine-induced lipolysis in adipocytes.Yakugaku zasshi journal of the Pharmaceutical Society of Japan 09/2008; 128(8):1195-201. · 0.39 Impact Factor -
Article: Effects of mannooligosaccharides from coffee mannan on blood pressure in Dahl salt-sensitive rats.
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ABSTRACT: Dahl salt-sensitive (Dahl-S) rats, serving as a model of hereditary hypertension, were used to examine the effect of mannooligosaccharides (MOS) on blood pressure. Dahl-S rats were induced to develop hypertension by administering them with a 1.25% salt solution ad libitum. In a 10-wk experimental period, the Dahl-S control and MOS groups developed and maintained significantly higher blood pressure than the Dahl salt-resistant normal control group. The MOS group showed a significantly lower blood pressure than the Dahl-S control group after 5-wk of treatment (p<0.05). In addition, the serum aldosterone level of the MOS group significantly decreased (p<0.05). The findings of this study using a model of hypertensive rats suggest that MOS are able to suppress an elevation in blood pressure.Journal of Nutritional Science and Vitaminology 04/2008; 54(2):181-4. · 1.20 Impact Factor -
Article: Anti-obesity effects of escins extracted from the seeds of Aesculus turbinata BLUME (Hippocastanaceae).
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ABSTRACT: To investigate the anti-obesity effects of escins extracted from the seeds of Aesculus turbinata BLUME, anti-obesity models in vitro and in vivo were employed. In a preliminary experiment, different solvent fractions of Aesculus turbinata BlUME as well as two isolated compounds were tested for their effects on pancreatic lipase (PL) in vitro. Subsequently, female ICR mice were fed a high fat diet with or without different concentrations of total escins for 11 weeks to examine body weight, parametrial adipose tissue weight, and hepatic triacylglycerol (TG) and total cholesterol (TC) contents. Plasma triacylglycerol levels (TG) after oral administration of lipid emulsions to rats were also investigated. The results showed that total escins (1 mg/ml) as well as two compounds isolated from total escins, namely escin Ib and IIa, showed inhibitory effects on PL activity. In vivo, total escins suppressed the increase in body weight, parametrial adipose tissue weight, TG content, and TC content in mice's liver; TG content in rat plasma was also reduced at 1, 2 and 3 h after oral administration of the lipid emulsion plus different concentrations of escins compared to those in the lipid emulsion groups. Meanwhile, mice fed a high fat diet plus 2% total escins for 3 d had an increased TG level in the feces compared to the HF group. The reason for this may be due to a delay in the intestinal absorption of dietary fat by inhibiting PL activity.CHEMICAL & PHARMACEUTICAL BULLETIN 02/2008; 56(1):12-6. · 1.59 Impact Factor -
Article: Reduction of fat storage in mice fed a high-fat diet long term by treatment with saponins prepared from Kochia scoparia fruit.
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ABSTRACT: The fresh fruit (Japanese name, Tonburi) of Kochia scoparia has been used as a food garnish in Japanese-style dishes from ancient times, and may prevent metabolic syndromes such as hyperlipidemia, hypertension, obesity and atherosclerosis. This study was performed to clarify whether an ethanol extract of K. scoparia fruit prevented obesity induced in mice by a high-fat diet for 9 weeks. The ethanol extract of K. scoparia fruit prevented the increases in body weight and parametrial adipose tissue weight induced by the high-fat diet. Furthermore, consumption of a high-fat diet containing 1% or 3% K. scoparia extract significantly increased the fecal content and the fecal triacylglycerol level at day 3 compared with those in the high-fat diet group. The ethanol extract (250 mg/kg) and total saponins (100 mg/kg) of K. scoparia inhibited the elevation of the plasma triacylglyccerol level 2 or 3 h after the oral administration of the lipid emulsion. Total saponins, momordin Ic, 2'-O-beta-d-glucopyranosyl momordin Ic and 2'-O-beta-d-glucopyranosyl momordin IIc isolated from K. scoparia fruit inhibited the pancreatic lipase activity (in vitro). These findings suggest that the anti-obesity actions of K. scoparia extract in mice fed a high-fat diet may be partly mediated through delaying the intestinal absorption of dietary fat by inhibiting pancreatic lipase activity.Phytotherapy Research 11/2006; 20(10):877-82. · 2.09 Impact Factor -
Article: [Isolation of pancreatic lipase activity-inhibitory component of spirulina platensis and it reduce postprandial triacylglycerolemia].
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ABSTRACT: In the process of investigating the hypolipidemic effects of Spirulina platensis, we found that the aqueous extract of S. platensis may inhibit the intestinal absorption of dietary fat by inhibiting pancreatic lipase activity. The aqueous extract of S. platensis (500 m/kg) reduced the elevation of rat plasma triacylglycerol levels after oral administration of the lipid emulsion 2 h after administration. To clarify the hypolipidemic effects of S. platensis, the active component was isolated and designated 1'-O-(palmitonyl)-2'-O-(caprylonyl) glyceryl-beta-alpha-D-galactopyranoside (glycolipid H-b2). Glycolipid H-b2 was found to inhibit pancreatic lipase activity in a dose-dependent manner. The fractions containing glycolipid H-b2 (250 mg/kg) reduced the elevation of rat plasma triacylglycerol levels after oral administration of the lipid emulsion 2 h after administration. Furthermore, we examined the effects of phycocyanin isolated from S. platensis on pancreatic lipase activity. Phycocyanin inhibited the pancreatic lipase activity in a dose-dependent manner. These results suggest that the inhibitory effects of S. platensis on postprandial triacylglycerolemia may be due in part to the inhibition of pancreatic lipase activity by glycolipid H-b2 and phycocyanin.Yakugaku zasshi journal of the Pharmaceutical Society of Japan 02/2006; 126(1):43-9. · 0.39 Impact Factor -
Article: Effects of compounds in leaves of Salix matsudana on arachidonic acid metabolism.
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ABSTRACT: Apigenin 7-O-beta-D-glucopyranuronide (1), luteolin 7-O-beta-D-glucopyranuronide (2), m-hydroxybenzyl beta-D-glucoside (3), and chrysoeriol 7-O-beta-D-glucopyranuronide (4) were isolated for the first time from the leaves of Salix matsudana. Furthermore, the effects of compounds 1, 2 and 3 on arachidonic acid metabolism were studied. These compounds inhibited significantly the production of 12-hydroxy-5, 8, 10, 14-eicosatetraenoic acid (12-HETE). In addition, the aglycon apigenin inhibited not only 12-HETE but also thromboxane B(2) (TXB(2)). The effect of compound (4) on arachidonic acid metabolism is now under investigation.Yakugaku zasshi journal of the Pharmaceutical Society of Japan 01/2006; 125(12):1005-8. · 0.39 Impact Factor -
Article: Effects of Coleus forskohlii on fat storage in ovariectomized rats.
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ABSTRACT: The antiobesity effects of Coleus forskohlii were investigated in ovariectomized (ovx) rats. Eight-week-old female Wistar rats were assigned to four groups: a sham-operated group fed the control diet (MF, sham-m) ; an ovx-m group fed the control diet; a sham-operated group fed the control diet containing 50 g/kg of Coleus forskohlii extract (sham-c) ; and an ovx-c group fed the control diet containing 50 g/kg of Coleus forskohlii extract. The body weight, adipose tissues, and cell diameter were investigated in ovx rats after Coleus forskohlii extract treatment. Administration of Coleus forskohlii extracts reduced body weight, food intake, and fat accumulation in ovx rats. Our results suggest that Coleus forskohlii may be useful in the treatment of obesity.Yakugaku zasshi journal of the Pharmaceutical Society of Japan 06/2005; 125(5):449-53. · 0.39 Impact Factor -
Article: [Antiobesity actions of Zingiber officinale Roscoe].
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ABSTRACT: Zingiber officinale Roscoe has been used as a folk medicine in China. An aqueous extract of Z. officinale Roscoe inhibited the hydrolysis of triolein emulsified with phosphatidylcholine by pancreatic lipase in vitro and it reduced the elevation of rat plasma triacylglycerol levels 1 and 2 h after oral administration of a lipid emulsion containing corn oil. These results suggest that the aqueous extract of Z. officinale Roscoe might inhibit the intestinal absorption of dietary fat by inhibiting its hydrolysis. Therefore we investigated the antiobesity effects of the aqueous extract of Z. officinale Roscoe by feeding a high-fat diet to mice for 8 weeks. Body weights at 2-8 weeks and final parametrial adipose tissue weights were significantly lower in mice fed the high-fat diet containing 3% aqueous extract of Z. officinale Roscoe than in the controls fed the high-fat diet. Feeding a high-fat diet containing 1% aqueous extract of Z. officinale Roscoe also significantly reduced final parametrial adipose tissue weights that were elevated in mice fed the high-fat diet alone. Our data suggest that the antiobesity effect of aqueous extract of Z. officinale Roscoe in mice fed a high-fat diet may be due in part to the inhibition of intestinal absorption of dietary fat by the active compounds of Z. officinale Roscoe.Yakugaku zasshi journal of the Pharmaceutical Society of Japan 03/2005; 125(2):213-7. · 0.39 Impact Factor -
Article: In vitro inhibitory effect of triterpenoidal saponins from Platycodi Radix on pancreatic lipase.
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ABSTRACT: In the process of investigating anti-obesity effect of Platycodi Radix, we found that aqueous extract of Platycodi Radix might inhibit intestinal absorption of dietary fat by inhibiting pancreatic lipase (PL) activity. In order to clarify the anti-obesity mechanism of Platycodi Radix, activity-guided isolation was performed to find active components. The total saponin fraction of Platycodi Radix appeared to have a potent inhibitory activity against the hydrolysis of triolein emulsified with phosphatidycholine by pancreatic lipase in vitro. Based on these results, further purification of active components yielded 10 known triterpenoidal saponins, among these compounds, platycodin A, C, D, and deapioplatycodin D exhibited significant inhibitory effects on PL at the concentration of 500 microg/mL with 3.3, 5.2, 34.8, and 11.67% pancreatic lipase activity vs control, respectively. Platycodin D was found to inhibit the PL activity in a dose-dependent manner. Therefore, the anti-obesity effect of Platycodi Radix might be due to the inhibition of pancreatic lipase by its saponins.Archives of Pharmacal Research 03/2005; 28(2):180-5. · 1.59 Impact Factor -
Article: Anti-obesity effects of chikusetsusaponins isolated from Panax japonicus rhizomes.
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ABSTRACT: The rhizomes of Panax japonicus are used as a folk medicine for treatment of life-style related diseases such as arteriosclerosis, hyperlipidemia, hypertension and non-insulin-dependent diabetes mellitus as a substitute for ginseng roots in China and Japan. Obesity is closely associated with life-style-related diseases. This study was performed to clarify whether chikusetsusaponins prevent obesity induced in mice by a high-fat diet for 9 weeks. We performed two in vivo experiments. In one, female ICR mice were fed a high-fat diet with or without 1 or 3% chikusetsusaponins isolated from P. japonicus rhizomes for 9 weeks. In the other, lipid emulsion with or without chikusetsusaponins was administered orally to male Wistar rats, and then the plasma triacylglycerol level was measured 0.5 to 5 h after the orally administered lipid emulsion. For in vitro experiments, the inhibitory effects of total chikusetsusaponins and various purified chikusetsusaponins on pancreatic lipase activity were determined by measuring the rate of release of oleic acid from triolein in an assay system using triolein emulsified with lecithin. Total chikusetsusaponins prevented the increases in body weight and parametrial adipose tissue weight induced by a high-fat diet. Furthermore, consumption of a high-fat diet containing 1 or 3% total chikusetsusaponins significantly increased the fecal content and triacylglycerol level at day 3 compared with the high-fat diet groups. Total chikusetsusaponins inhibited the elevation of the plasma triacylglycerol level 2 h after the oral administration of the lipid emulsion. Total chikusetsusaponins, chikusetsusaponin III, 28-deglucosyl-chikusetsusaponin IV and 28-deglucosyl-chikusetsusaponin V inhibited the pancreatic lipase activity. The anti-obesity effects of chikusetsusaponins isolated from P. japonicus rhizomes in mice fed a high-fat diet may be partly mediated through delaying the intestinal absorption of dietary fat by inhibiting pancreatic lipase activity. The present study clearly indicated that the saponin fractions of P. japonicus rhizomes had a significant anti-obesity action and supports the traditional usage as a substitute drug for ginseng roots.BMC Complementary and Alternative Medicine 02/2005; 5:9. · 2.24 Impact Factor -
Article: [Effects of long-term administration of caffeine on fat storage in ovariectomized rats].
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ABSTRACT: It is well known that obesity occurs in women with climacteric disorders. The aim of this study was to examine whether caffeine prevents obesity and bone loss in ovariectomized rats (ovx). Eight-week-old female Wistar rat were assigned to 4 groups: a sham-operated group fed the control diet (CE-2); an ovx-c group fed the control diet; an ovx-caf 0.15% group fed the control diet containing 1.5 g/kg of caffeine; and an ovx-caf 0.3% group fed the control diet containing 3 g/kg of caffeine. Body weights at 2-9 weeks and the final parametrail adipose tissue weights were significantly lower in the ovx-caf 0.3% group than in the ovx-c group. Food intakes were significantly lower in the ovx-caf 0.3% group than in the ovx-c group. After 9 weeks, the rats were killed and adipose tissues were sampled immediately. Basal lipolysis was increased in the ovx-caf 0.3% group fed the control diet containing 3 g/kg of caffeine than in the ovx-c group fed the control diet. The relative content of calcium (g/100 g body weight) in the ovx-caf 0.3% group was significantly increased compared with that in the ovx-c group. These results show a new possible role for caffeine in the prevention of lifestyle-related diseases.Yakugaku zasshi journal of the Pharmaceutical Society of Japan 12/2004; 124(11):841-6. · 0.39 Impact Factor -
Article: [Effects of the flavonoid fraction from Ginkgo biloba extract on the postprandial blood glucose elevation in rats].
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ABSTRACT: The present study investigated the effects of Ginkgo biloba extract and its flavonoid fractions on alpha-amylase and alpha-glucosidase activity in vitro. Ginkgo biloba extracts and their flavonoid fraction significantly inhibited alpha-amylase and alpha-glucosidase activity in vitro. Furthermore, Ginkgo biloba extracts and their flavonoid fraction reduced the elevation of rat plasma glucose level after oral administration of various saccharinity agents. In addition, we examined the effects of the flavonoid fraction isolated from Ginkgo biloba extracts on the plasma glucose level in streptozotocin-induced diabetic rats. When flavonoid fractions were orally administered to the rats three times daily for 9 days, plasma glucose concentrations were decreased compared with those in the water treatment group. Furthermore, flavonoid fractions reduced the elevation of rat plasma glucose levels after oral administration of sucrose and glucose in streptozotocin-induced diabetic rats.Yakugaku zasshi journal of the Pharmaceutical Society of Japan 10/2004; 124(9):605-11. · 0.39 Impact Factor -
Article: New biologically active triterpenoid saponins from Scabiosa tschiliensis.
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ABSTRACT: Eleven new triterpenoid saponins, scabiosaponins A-K (1-11), and hookerosides A (12) and B (13) were isolated from the whole plants of Scabiosa tschiliensis. The structures of the new compounds were established on the basis of extensive NMR (DEPT, DQF-COSY, HETCOR, TOCSY, HMQC, HMQC-TOCSY, HMBC, and NOESY) and MS studies coupled with chemical degradations. The biological activity of compounds 1-10, 12, and 13 and prosapogenin 1b were examined against pancreatic lipase. Scabiosaponins E, F, G, I (5, 6, 7, 9), hookerosides A, B (12, 13), and prosapogenin 1b all exhibited strong inhibition of pancreatic lipase in vitro.Journal of Natural Products 05/2004; 67(4):604-13. · 3.13 Impact Factor -
Article: Anti-obesity action of Salix matsudana leaves (Part 1). Anti-obesity action by polyphenols of Salix matsudana in high fat-diet treated rodent animals.
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ABSTRACT: In preliminary experiments, polyphenol fractions prepared from the leaves of Salix matsudana reduced the elevation of the rat plasma triacylglycerol level at 3 and 4 h after oral administration of a lipid emulsion containing corn oil, at a dose of 570 mg/kg. The present study examined the anti-obesity action of polyphenol fractions of S. matsudana leaves by testing whether the polyphenol fractions prevented the obesity induced by feeding a high-fat diet to female mice for 9 weeks. Body weights at 2-9 weeks and the fi nal parametrial adipose tissue weights were significantly lower in mice fed the high-fat diet with 5% polyphenols of S. matsudana leaves than in those fed the high-fat diet alone. The polyphenols of S. matsudana leaves also significantly reduced the hepatic total cholesterol content, which was elevated in mice fed the high-fat diet alone. In addition, the polyphenol fractions of S. matsudana leaves inhibited palmitic acid uptake into brush border membrane vesicles prepared from rat jejunum and alpha-amylase activity, and their fractions enhanced norepinephrine-induced lipolysis in fat cells. In conclusion, it is suggested that the inhibitory effects of the flavonoid glycoside fraction of S. matsudana leaves on high-fat diet-induced obesity might be due to the inhibition of carbohydrate and lipid absorption from small intestine through the inhibition of alpha-amylase and palmitic acid uptake into small intestinal brush border membrane or by accelerating fat mobilization through enhancing norepinephrine-induced lipolysis in fat cells.Phytotherapy Research 01/2004; 17(10):1188-94. · 2.09 Impact Factor -
Article: Anti-obesity action of Salix matsudana leaves (Part 2). Isolation of anti-obesity effectors from polyphenol fractions of Salix matsudana.
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ABSTRACT: Previously, it was reported that polyphenol fractions prepared from the leaves of Salix matsudana reduced the elevation of the rat plasma triacylglycerol level at 3 and 4 h after oral administration of a lipid emulsion containing corn oil, at a dose of 570 mg/kg. Moreover, body weights at 2-9 weeks and the fi nal parametrial adipose tissue weights were significantly lower in mice fed the high-fat diet with 5% polyphenol fractions of S. matsudana leaves than in those fed the high-fat diet alone. The polyphenol fractions of S. matsudana leaves also significantly reduced the hepatic total cholesterol content, which was elevated in mice fed the high-fat diet alone. In addition, the polyphenol fractions of S. matsudana leaves inhibited palmitic acid uptake into brush border membrane vesicles prepared from rat jejunum and alpha-amylase activity, and their fractions enhanced norepinephrine-induced lipolysis in fat cells. To clarify the active substances inhibiting the palmitic acid uptake into small intestinal brush border membrane, the alpha-amylase activity or enhancing the norepinephrine-induced lipolyis in fat cells, the isolation of the active substances from polyphenol fraction was attempted using the above three assay systems. Compounds 1, 2 and 3 were isolated from the polyphenol fractions and identified as apigenin-7-O-d-glucoside, luteolin-7-O-d-glucoside and chrysoeriol-7-O-d-glucoside, respectively. Among three flavonoids, apigenin-7-O-d-glucoside inhibited alpha-amylase activity, and luteolin-7-O-d-glucoside and chrysoeriol-7-O-d-glucoside inhibited palmitic acid uptake into small intestinal brush border membrane. Furthermore, three flavonoid glucosides enhanced norepinephrine-induced lipolysis in fat cells.Phytotherapy Research 01/2004; 17(10):1195-8. · 2.09 Impact Factor -
Article: Inhibition of lipase activities by citrus pectin.
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ABSTRACT: The oral administration of pectin to rats reduced and delayed the peak plasma triacylglycerol concentration. Pectin inhibited the hydrolysis of trioleoylglycerol emulsified with soybean phosphatidylcholine by pancreatic, carboxylester, and lingual lipases in a concentration-dependent manner. However, the effective concentration of pectin for lingual lipase was 100 times lower than that for pancreatic lipase. Pectin did not inhibit the tributyrin- and p-nitrophenylbutyrate-hydrolyzing activities by pancreatic and carboxylester lipase. When low molecular weight pectin was assayed, pectin at a molecular weight of 90,000 (MW 90) most strongly inhibited three lipase activities. When the effect of pH on pectin inhibition was analyzed using pancreatic lipase, strong inhibition was observed at an acidic pH (below pH 7.0). In the assay system, the pancreatic lipase protein levels in the supernatant and fat layer were estimated by Western blotting with an anti-pancreatic lipase antibody. Pectin reduced the amount of pancreatic lipase protein in the fat layer in a concentration-dependent manner and concomitantly increased that in the supernatant. These results suggest that pectin may interact with emulsified substrates and inhibit the adsorption of lipase to the surface of substrate emulsion.Journal of Nutritional Science and Vitaminology 11/2003; 49(5):340-5. · 1.20 Impact Factor -
Article: Saponins from platycodi radix ameliorate high fat diet-induced obesity in mice.
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ABSTRACT: We examined the effects of crude saponins isolated from Platycodi radix on the degree on fat storage induced in mice by feeding a high fat diet for 9 wk. We reported previously that feeding mice a high fat diet for a longer time caused obesity and fatty liver compared with those fed a low fat diet, nonpurified diet. Feeding a high fat diet containing 10 or 30 g/kg crude saponins prevented the body and parametrial adipose tissue weight increases and hepatic steatosis of mice fed the high fat diet alone. Furthermore, crude saponins (375 mg/kg) inhibited the elevations in blood triacylglycerol in rats orally administered a lipid emulsion compared with that of rats given the lipid emulsion alone. Previously, we reported that crude saponins inhibited pancreatic lipase activity in vitro. To identify the active substance(s) of crude saponins, we examined the effects of purified platycodin D, the primary saponin in the crude mixture, on pancreatic lipase activity and on the blood triacylglycerol elevation in rats administered the oral lipid emulsion tolerance test. Platycodin D (0.5 and 1.0 g/L) inhibited pancreatic lipase activity in vitro and at a dose of 244 mg/kg, inhibited the elevation of blood triacylglycerol. Therefore, the antiobesity effect of the crude saponins in mice fed a high fat diet may be due to the inhibition of intestinal absorption of dietary fat by platycodin D.Journal of Nutrition 09/2002; 132(8):2241-5. · 3.92 Impact Factor
Top co-authors
Top Journals
Institutions
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2012
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University of Electronic Science and Technology of China
- State Key Laboratory of Electronic Thin Films and Integrated Devices
Chengdu, Sichuan Sheng, China
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2002–2006
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Prefectural University of Kumamoto
Kumamoto-shi, Kumamoto Prefecture, Japan
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2003–2004
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Ehime University
- • Department of Medical Biochemistry
- • Integrated Center for Sciences
Matsuyama-shi, Ehime, Japan
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