Songsong Zhu

Sichuan University, Hua-yang, Sichuan, China

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Publications (41)78.9 Total impact

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    ABSTRACT: We retrospectively compared the clinical outcomes of autogenous coronoid process grafts (n=32) and costochondral grafts (n=28) in condylar reconstruction for the treatment of unilateral ankylosis of the temporomandibular joint (TMJ) in adults. Preoperative and postoperative assessments included diet scores, cone-beam computed tomography (CT), maximal interincisal opening, lateral excursion, and mandibular deviation on opening the mouth. There were no significant differences between the 2 groups in the measurements before and after the operation with respect to incisal opening, lateral excursion, mandibular deviation, diet scores, or recurrence rate, but in both the postoperative incisal opening, lateral excursion, and diet scores had improved significantly compared with preoperatively. After costochondral graft 3 patients developed intraoperative plural tears, and 6 had temporary pain at the donor site. The frontal branch of the facial nerve was temporarily affected in 5 patients after costochondral graft and 3 after coronoid process grafts, all of which recovered in 3-6 months. There was no recurrence after coronoid process grafting, and one after costochondral grafting. The clinical outcomes in both groups were satisfactory and comparable. Autogenous coronoid process grafting may therefore be a good alternative for condylar reconstruction in patients with ankylosis of the TMJ.
    British Journal of Oral and Maxillofacial Surgery 09/2014; · 2.72 Impact Factor
  • Jian Song, Songsong Zhu, En Luo, Jing Hu, Ge Feng
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    ABSTRACT: This study was designed to investigate the stress and the displacement distributions of the mandible after mandibular angle ostectomy (MAO) by means of three-dimensional finite element analysis.
    Journal of Craniofacial Surgery 07/2014; 25(4):e375-e378. · 0.69 Impact Factor
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    ABSTRACT: Purpose: The objective of this study was to investigate cancellous bone response to strontium-doped hydroxyapatite (SrHA) in ovariectomized (OVX) rats. Methods: Hydroxyapatite (HA) and 10%SrHA (HA with 10 mol% calcium substituted by strontium) implants were prepared and characterized by scanning electronic microscopy (SEM), energy dispersive microanalysis (EDX) and X-ray diffraction (XRD). Twelve weeks after bilateral ovariectomy, 20 rats randomly received HA or 10%SrHA implants in the right distal femur, with 10 animals in each group. Eight weeks after implantation, specimens were harvested and analyzed by micro-computed tomography (micro-CT) and histology.ResultsCompared with HA, 10%SrHA raised the percentage bone volume by 42.6%, bone-to-implant contact by 47.1%, mean trabecular number by 27.3%, mean trabecular thickness by 31.5% and mean connectivity density by 37.4%, while it decreased mean trabecular separation by 20.1% in micro-CT evaluation. 10%SrHA also increased the bone area density by 47.6% in histological analysis. Conclusions: With the HA implants as controls, the 10%SrHA implants were shown to increase bone density and bone-to-implant contact, and improve trabecular architecture in the vicinity of implant surfaces.
    Journal of applied biomaterials & functional materials. 04/2014;
  • Xianwen Liu, Songsong Zhu, Jing Hu
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    ABSTRACT: The purpose of this systematic review is to evaluate the efficacy of a new modified alar base cinch suture by comparing it with the commonly used classic alar base suture after LeFort I osteotomy. A comprehensive search strategy was performed to include interventional studies involving the comparisons of alar base suturing methods after LeFort I osteotomy. Data analyses were conducted using the random-effects model. Three studies with 146 participants undergoing LeFort I maxillary osteotomy were included in this review. The results showed that, compared with the classic method, both modified transseptal alar base suture and modified reinsertion sutures significantly decreased postoperative alar and alar base widening. The modified alar base cinch suture was more effective than the classic alar base suture in maintaining preoperative alar and alar base width after LeFort I osteotomy.
    Oral surgery, oral medicine, oral pathology and oral radiology. 01/2014; 117(1):37-44.
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    ABSTRACT: This study was to investigate whether surgically created incisions in discs at different sizes would heal spontaneously. Thirty mature goats were randomly divided into group A (1mm), group B (3mm) and group C (5mm) according to the length of incision on the discs. Five animals in each group were sacrificed at 3 and 6 months postoperatively. The disc and condyle were evaluated by gross, histological and immunohistochemistry examinations. 1-mm discal incisions healed spontaneously with nearly normal gross and histological appearance at 6 months postoperatively, while 3-mm and 5-mm discal incisions failed to heal. Degenerative changes were observed in the fibrocartilage in both 3-mm and 5-mm incision groups, with a greater extent in the 5-mm group. Our results suggested that the TMJ disc of goat has limited self-repair capability to damage, which will be useful for making pre-surgical decisions related to the size and healing period of TMJ disc perforations.
    Journal of Cranio-Maxillofacial Surgery 01/2014; · 1.61 Impact Factor
  • Hui Li, Jing Hu, En Luo, Songsong Zhu, Jihua Li
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    ABSTRACT: Purpose Osteochondroma is a benign tumor that is rare in the craniofacial region. When it occurs, the condyle and the coronoid process are the most commonly affected sites. The secondary progressive malocclusion and facial asymmetry are common physical signs in most cases. The traditional surgical treatment for osteochondroma of the mandibular condyle has been condylectomy with or without reconstruction of the condyle. We opine that more attention should be paid in restoring the joint function, improving facial appearances and correcting malocclusion, as well as re-establishing harmony among them. Patients and Methods From Jan. 2000 to Mar. 2013, 27 patients (17 female and 10 male) who were diagnosed with osteochondroma of unilateral mandibular condyle underwent condylectomy and condylar reconstruction by use of pedicled posterior mandibular border obtained by ramus osteotomy. The secondary dentofacial deformities were simultaneously corrected by use of orthognathic and facial contouring procedures, followed by orthodontic treatment when necessary. Occlusion and temporomandibular joint pain and function including maximal mouth opening and maximum protrusion were recorded preoperatively and postoperatively. Results :The patients were followed-up for an average of 13 months (range 24–48 months). The outcomes and the feedback information of patients showed apparent improved joint function with no cases of recurrence of osteochondroma. The secondary dentofacial deformities were corrected significantly. Satisfactory occlusion was achieved with orthognathic and orthodontic treatment. Conclusion Our data suggest that condylectomy and condylar reconstruction; simultaneous correction of the secondary dentofacial deformities by use of orthognathic procedures might be a better approach to manage osteochondroma accompanied by dentofacial deformities. In addition to facial contouring procedures, orthodontic treatment should be considered for better improvement in facial esthetics and occlusion.
    Journal of Oral and Maxillofacial Surgery. 01/2014;
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    ABSTRACT: In cartilage tissue engineering using stem cells, it is important to stimulate proliferation and control the differentiation of stem cells to specific lineages. Here we reported a combined technique for articular cartilage repair, consisting of bone marrow mesenchymal stem cells (BMMSCs) transfected with connective tissue growth factor (CTGF) gene and NaOH-treated poly(lactic-co-glycolic) acid (PLGA) scaffolds. In the present study, BMMSCs or CTGF-modified BMMSCs seeded on PLGA or NaOH-treated PLGA scaffolds were incubated in vitro and NaOH-treated PLGA significantly stimulated proliferation of BMMSCs, while CTGF gene transfer promoted chondrogenic differentiation. The effects of the composite on the repair of cartilage defects were evaluated in rabbit knee joints in vivo. Full-thickness cartilage defects (diameter: 5 mm; depth: 3 mm) were created unilaterally in the patellar groove. Defects were either left empty (n = 18) or implanted with BMMSCs/PLGA (n = 18), BMMSCs/NaOH-treated PLGA (n = 18), or CTGF-modified BMMSCs/NaOH-treated PLGA (n = 18). The defect area was examined grossly, histologically, and mechanically at 6, 12, and 24 weeks postoperatively. Implanted cells were tracked using adeno-LacZ labeling at 6 weeks after implantation. Overall, the CTGF-modified BMMSCs/NaOH-treated PLGA group showed successful hyaline-like cartilage regeneration similar to normal cartilage, which was superior to the other groups using gross examination, qualitative and quantitative histology, and mechanical assessment. The in vivo viability of the implanted cells was demonstrated by their retention for 6 weeks after implantation. These findings suggested that a combination of CTGF-modified BMMSCs and NaOH-treated PLGA may be an alternative treatment for large osteochondral defects in high-loading sites.
    Cell Transplantation 01/2014; 23(6):715-27. · 4.42 Impact Factor
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    ABSTRACT: PURPOSE: To compare the efficacy of operative and conservative treatment of displaced condylar fractures of the mandible, a meta-analysis was performed. STUDY DESIGN: PubMed, Cochrane Controlled Trials Register, EMBASE electronic databases were searched until 10 December 2012. English studies were restricted in randomized controlled trials (RCT). RESULTS: Four RCT with 177 patients were included. No significant difference was found between the 2 groups in the maximal interincisal opening (P = .32). However a statistically significant difference was seen that operative treatment statistically got better efficacy in lateral excursion movement, protrusion, malocclusion, and temporomandibular joint pain (P < .05). CONCLUSIONS: This meta-analysis confirms that both treatment options for unilateral displaced condylar fractures of the mandible yielded acceptable results. However, operative treatment was superior in most objective and subjective functional parameters.
    Oral surgery, oral medicine, oral pathology and oral radiology. 05/2013;
  • Binbin Ying, Jing Hu, Songsong Zhu
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    ABSTRACT: This study introduced the modified Leclerc blocking procedure with miniplate and temporal fascial flap for recurrent temporomandibular joint (TMJ) dislocation and evaluated its clinical effects. Seven patients were treated by the modified Leclerc blocking procedure with miniplate and temporal fascial flap. The postoperative follow-up period ranged from half a year to 2 years to access the maximal mouth opening, TMJ disorder symptoms (pain and sound), and incidence of recurrence. No recurrence was observed in all of the 7 patients postoperatively. The mean preoperative and postoperative MMOs were 49.7 mm and 40.1 mm, respectively. There were 3 patients who reported the alleviation of pain and/or sound postoperatively. Two older patients with long-term course of disease reported no improvement of the TMJ symptoms in terms of pain and sound postoperatively. Our results showed that the modified Leclerc blocking procedure with miniplates and temporal fascial flap provided a more stable support for the condylar movement with less recurrence, suggesting that this operation could be a good alternative for the treatment of recurrent TMJ dislocation.
    The Journal of craniofacial surgery 05/2013; 24(3):740-742. · 0.81 Impact Factor
  • Songsong Zhu, Dazhang Wang, Qiudan Yin, Jing Hu
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    ABSTRACT: Temporomandibular joint (TMJ) ankylosis with secondary dentofacial deformities in adult patients is a severely disfiguring condition and surgical treatment of this disease remains a great clinical challenge. Treatment goals are to restore the joint function, to improve facial appearances and to correct malocclusion, as well as to re-establishing harmony among them. Currently, various surgical techniques, such as arthroplasty with or without interpositional material, orthognathic surgery, distraction osteogenesis, autologous bone or bone replacement materials graft and plastic surgery, have been described in the literature. In most cases these techniques should be used in combination to achieve satisfactory outcomes. The biggest difficulty for most clinicians is to determine the proper sequence of these procedures because no uniform treatment protocol has been established. Based on the published literature and our own clinical experiences, we have prepared this review article to provide some guidelines for the surgical management of TMJ ankylosis with dentofacial deformities in adults, which will be modified and updated periodically to provide the best treatment options to benefit our patients.
    Journal of cranio-maxillo-facial surgery: official publication of the European Association for Cranio-Maxillo-Facial Surgery 01/2013; · 1.25 Impact Factor
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    ABSTRACT: Osteoporosis (OP) and osteoarthritis (OA) are major health problems in the increasing elderly population, particularly in postmenopausal women, but their relationship remains unclear. The present study investigated whether alendronate (ALN), a potent inhibitor of bone resorption, could protect articular cartilage from degeneration in a combined animal model of OP and OA induced by ovariectomy (OVX). Seventy-eight seven-month-old female Sprague-Dawley rats were assigned into five experimental groups: (1) sham-operated with vehicle treatment, (2) sham-operated with ALN treatment, (3) OVX with vehicle treatment, (4) ALN treatment starting at OVX, and (5) ALN treatment starting at eight weeks after OVX. Histological and micro-CT analyses, together with urine collagen degradation markers, indicated that early ALN treatment completely prevented both subchondral bone loss and cartilage surface erosion induced by OVX. Although late ALN treatment also inhibited subchondral bone loss and significantly reduced cartilage erosion in the OVX rats, these tissues did not completely recover even after 10-weeks of ALN treatment. Quantitative RT-PCR analyses showed that the protective effect of ALN correlated with increased ratio of OPG/RANKL in both subchondral bone and cartilage. Moreover, whereas OVX caused upregulation of expression of matrix metalloproteinases MMP-13 and MMP-9 in the articular cartilage and chondrocytes in the interface between the articular cartilage and subchondral bone, respectively, early ALN treatment blocked whereas late ALN treatment attenuated the upregulation of these catabolic enzymes in the corresponding tissues. Together, these data indicate that the subchondral bone loss plays an important role in OA pathogenesis in the combined OP and OA model and suggest that treatment timing is an important factor for the effectiveness of anti-resorptive drugs therapy of combined OP and OA.
    Bone 01/2013; · 4.46 Impact Factor
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    ABSTRACT: Surgical correction of craniosynostosis in children is associated with substantial intraoperative bleeding. Intraoperatively administered tranexamic acid (TXA) can lessen blood loss during orthopedic and cardiovascular surgery, but its efficacy in craniosynostosis surgery is uncertain. Therefore, a meta-analysis performed with published comparative studies was to determine whether TXA could reduce packed red blood cells (or erythrocytes) (PRBCs) transfused and blood loss during pediatric craniosynostosis surgery. Two PubMed and EMBASE electronic databases were searched until June 2012. Eligible studies were restricted in comparative controlled trials. Four studies in 3 articles with 138 patients were included. The results showed that intraoperative administration of TXA can significantly reduce transfusion of PRBCs (weighed mean difference [WMD] = -10.81, 95% confidence interval [CI] = -16.84 to -4.78, P < 0.00001). In the level of blood loss, the meta-analysis on 4 studies showed that the difference was statistically significant (WMD = -20.53, 95% CI = -32.26 to -8.80, P = 0.0006) between the TXA groups and the control groups. However, the subgroup analysis on randomized controlled trials showed that TXA did not significantly reduce blood loss during surgery compared with the placebo group (WMD = -30.79, 95% CIs = -71.72 to 10.14, P = 0.14). Tranexamic acid can significantly reduce the transfusion of PRBCs in children undergoing craniosynostosis surgery. However, there is a controversy on the efficacy of TXA in reducing blood loss. Therefore, new randomized controlled trials to assess the effects of TXA in children with craniosynostosis surgery should be conducted.
    The Journal of craniofacial surgery 01/2013; 24(1):299-303. · 0.81 Impact Factor
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    ABSTRACT: Schwannomas (neurilemmomas) are benign nerve sheath tumor originating from Schwann cells. They are well circumscribed and rarely infiltrate and metastasize. Schwannomas of the head and neck commonly occur in the tongue followed by the palate, floor of mouth, buccal mucosa, and mandible. Tongue base schwannomas could extend to the pharyngeal cavity or the floor of the mouse, and it is difficult to differentiate between tumors of the lingual, hypoglossal, and glossopharyngeal nerves.Surgical treatment of tongue base schwannomas is difficult because of limited operative exposure. Although mandibulotomy with lip splitting could obtain good exposure, surgeons might strike a balance between exposure obtaining and morbidity following because there are intricate neurovascular anatomical relationships in this region, and mandibulotomy with lip splitting would cause significant morbidity. Surgical approach options are important for tongue base schwannoma removal. From March 2008 to March 2010, 8 patients were clinically and pathologically diagnosed with tongue base schwannomas in our department, and all underwent surgical treatment. In our experience, transoral approach was used for tongue base schwannomas extending to the floor of the mouse and suprahyroid pharyngotomy approach for those extending to the pharyngeal cavity. Follow-up was made until now. One patient who experienced transoral excision still experienced numbness in the region of the lateral tongue tip, and the other 7 patients had no postoperative long-term complications.
    The Journal of craniofacial surgery 01/2013; 24(1):e9-e11. · 0.81 Impact Factor
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    ABSTRACT: OBJECTIVE: The objective of this article was to evaluate the efficacy of tranexamic acid on blood loss in orthognathic surgery. A meta-analysis was performed. STUDY DESIGN: The PubMed and EMBASE electronic databases were searched until June 30, 2012. Eligible studies were restricted to randomized controlled trials (RCTs). RESULTS: Four RCTs with 183 patients were included. The results showed that intraoperative blood loss in the tranexamic acid group was statistically reduced (weighted mean difference [WMD] = -93.56, 95% CI = -132.59-54.52, P < .00001). However, the postoperative levels of hemoglobin (Hb) and hematocrit (Hct) have no significant difference compared with placebo groups (WMD = 0.50, 95% CIs = -0.43-1.43, P = .29 and WMD = 0.18, 95% CIs = -1.64-1.99, P = .85, respectively). CONCLUSIONS: This meta-analysis confirms that tranexamic acid can effectively reduce intraoperative blood loss in orthognathic surgery, especially by intravenous administration. But, tranexamic acid cannot affect postoperative levels of Hb and Hct.
    Oral surgery, oral medicine, oral pathology and oral radiology. 12/2012;
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    ABSTRACT: The nervous system plays an important role in bone metabolism. However, the effect of denervation on bone formation during distraction osteogenesis (DO) remains unclear. We studied neural influence on bone regeneration during DO in a rabbit model. 24 New Zealand male white rabbits underwent left tibial osteodistraction. Before distraction, the animals were randomly divided into group R (resected left sciatic nerve) and group I (intact left sciatic nerve). 8 weeks after completion of distraction, the animals were killed and the lengthened tibias were harvested for radiography, micro-CT, histological evaluation, and mechanical testing. New regenerated bone was present in the distraction gaps of all animals at the end of the study, as revealed by radiography, micro-CT, and histology. However, less new bone formation and a lower degree of mineralization were observed in group R. The mechanical strength of the distraction gap in group I was 1.3-fold greater than that in group R when measured using the 3-point bending test. The results suggest that the nervous system plays an essential role during DO: the denervation appears to have an inhibitory effect on bone formation.
    Acta Orthopaedica 08/2012; 83(4):406-10. · 2.74 Impact Factor
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    ABSTRACT: A mouth floor mucosal flap was developed to reconstruct medium-sized intraoral buccal defects, too large for primary closure if major functional and aesthetic impairment is to be avoided. Although free flaps, perforator flaps, or even skin grafts can be designed for buccal reconstruction, they may not provide good mucosal sensitivity, motility, volume, and texture to replace lost structures with similar tissue. Moreover, secondary morbidity can be avoided with this adjacent flap.In our study, 8 flap reconstruction procedures were performed from March 2009 to July 2011: 4 cases of leukoderma, 2 cases of buccal cancer (T1 N0 M0), and 2 cases of papillary epithelioma. The largest size amount to 5.3 × 3.8 cm (length × width), with a mean of 4.3 × 3.4 cm. Compared with free flaps (forearm arm flap for example) for buccal reconstruction, application of the mouth floor flap has its indications. First, to secure the motility of tongue and function of mouth opening, the upper bound of the defects was below the occlusion line. Generally, the width between top and bottom was less than 4 cm. Second, at least a partial buccinator muscle can be preserved after lesion resection and then facial collapse can be avoided. Third, patients had molar absence or relevant teeth had to be extracted during surgery.All patients recovered from intraoral surgeries with good objective and subjective speech and swallowing and aesthetics and without injury to the lingual nerve, the submandibular gland duct, and the sublingual gland. Results indicate that the mouth floor mucosal flap is reliable and technically easy for reconstructing medium-sized intraoral buccal defects, with good function and aesthetics with little secondary morbidity.
    The Journal of craniofacial surgery 07/2012; 23(4):1143-5. · 0.81 Impact Factor
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    ABSTRACT: Transforming growth factor-beta (TGF-β) plays an important part in the repair of cartilage in osteoarthritis. It has been hypothesised that intra-articular injection of TGF-β(1) promotes repair of cartilage and protects the subchondral bone from damage in osteoarthritic temporomandibular joints (TMJs). We made bilateral partial perforations of the disc to induce osteoarthritic joints in 36 rabbits. TGF-β(1) 20, 40, or 80ng were injected into the right joint, and vehicle alone was injected into the left joint. Four additional animals were used as normal controls. Microcomputed tomography was used to quantify the three-dimensional microarchitecture of subchondral bone, followed by assessment of the proteoglycan content. All joints treated with TGF-β(1) were covered by a layer of well-organised fibrocartilage, and had increased proteoglycan content and normal microarchitectural properties, whereas the joint treated by vehicle alone had typical osteoarthritis-related degradation of cartilage and sclerosis of subchondral bone. These results suggested that TGF-β(1) is an effective way of treating osteoarthritis of the TMJ.
    British Journal of Oral and Maxillofacial Surgery 07/2012; · 2.72 Impact Factor
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    ABSTRACT: Orthopedic surgeons and dentists often implant materials to repair bone tissue defects and restore physiological functions of bone organs. The clinical success depends on adequate bone formation in operation sites. However, the real cause of osteogenesis has not yet been fully elucidated. To investigate the bone response to implanted materials, this study examined the bone tissue reaction in rat femoral medullary canal, which received gelatin and collagen as foreign-body materials. A total of 36 six-month-old Sprague-Dawley rats were randomly and meanly divided into three groups. In the gelatin group, the bilateral femora received gelatin material; in the collagen group, they were implanted with type I collagen, and in the control group, the femora suffered from sham operation with no materials inserted. After 2, 4, 8, and 12 weeks, specimens were harvested and subjected to a series of examinations. After 2 weeks of healing, a significant upregulation of both alkaline phosphatase and osteocalcin by both kinds of implanted materials relative to the control (sham implantation group) was seen in gene expression analysis. Strong reactivity of osteoprotegerin and receptor activator of NFκB ligand was detected in the two test groups in immunohistochemistry at 4 weeks of healing. Also, micro-CT revealed an increase in cortical bone thickness in the two test groups as compared to the control group. Densitometry showed increased bone mineral density in the bone receiving materials after 12 weeks, leading to the enhanced maximum load in the test groups. These results indicated that the implanted materials led to an osteogenesis response in rat femoral medullary canal. Thus, we probably should reconsider the potential cascades of tissue reaction when utilizing orthopedic and dental implants and other materials to recover bone related-organ function and repair bone defects.
    Journal of Orthopaedic Science 06/2012; 17(5):626-33. · 0.96 Impact Factor
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    ABSTRACT: PURPOSE: Distraction osteogenesis can be used to correct micrognathia after temporomandibular joint (TMJ) ankylosis. However, there is still some controversy over the proper sequencing of management for the ankylosed patients. The objective of the present study was to evaluate a staged treatment of TMJ ankylosis accompanied by micrognathia using arthroplasty, mandibular distraction osteogenesis, and advancement genioplasty. PATIENTS AND METHODS: A total of 12 bilateral TMJ ankylosis patients with micrognathia (aged 17 to 27 years) underwent arthroplasty as the initial surgical procedure, followed by orthodontic treatment and correction of mandibular micrognathia by osteodistraction and advancement genioplasty as the second surgical procedure. The clinical results were evaluated by mouth opening, radiography, medical photography, and respiratory function. RESULTS: The patients were followed up for a minimum of 8 months to a maximum of 36 months. The TMJ ankylosis was released successfully in all the patients, showing an increase in average mouth opening from 3.3 mm preoperatively to 35.8 mm postoperatively. Micrognathia was corrected, and, remarkably, the obstructive sleep apnea and hypopnea syndrome was cured. Satisfactory occlusion was achieved with orthodontic treatment. CONCLUSIONS: Our data suggest that a staged and surgical orthodontic treatment might be a better approach to manage TMJ ankylosis accompanied by mandibular hypoplasia. In addition to mandibular osteodistraction, advancement genioplasty should be considered for better improvement in facial esthetics and respiratory function.
    Journal of oral and maxillofacial surgery: official journal of the American Association of Oral and Maxillofacial Surgeons 05/2012; · 1.58 Impact Factor
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    ABSTRACT: Strontium ranelate (SrR) was an effective anti-osteoporotic drug to increase bone formation and decrease bone resorption. However, reports about the effect of SR on osteoblastic and adipocytic differentiation from bone marrow mesenchymal stem cells (BMMSCs) are limited. The purpose of this study is to evaluate whether SrR affects the ability of BMMSCs to differentiate into osteoblasts or adipocytes. Rat BMMSCs were identified by flow cytometry and exposed to SR (0.1 and 1.0mMSr(2+)) under osteogenic or adipogenic medium for 1 and 2weeks. The proliferation and differentiation of BMMSCs were analyzed by MTT, alkaline phosphatase (ALP), Oil red O staining, quantitative real-time RT-PCR and Western blot assays. SrR significantly inhibited the proliferation, increased osteoblastic but decreased adipocytic differentiation of rat BMMSCs dose-dependently. In osteogenic medium, SrR increased the expression of ALP, the mRNA levels of Cbfa1/Runx2, bone sialoprotein, and osteocalcin by RT-PCR, and the protein levels of Cbfa1/Runx2 by Western blot. In adipogenic medium, SrR decreased the mRNA levels of PPARγ2, adipocyte lipid-binding protein 2 (aP2/ALBP), and lipoprotein lipase (LPL) by RT-PCR, and the protein expression of PPARγ in Western blot analysis. These results indicated that the effects of SrR to promote osteoblastic but inhibit adipocytic differentiation of BMMSCs might contribute to its effect on osteoporosis treatment.
    Biochemical and Biophysical Research Communications 02/2012; 418(4):725-30. · 2.28 Impact Factor

Publication Stats

230 Citations
78.90 Total Impact Points


  • 2006–2014
    • Sichuan University
      • • State Key Laboratory of Oral Diseases
      • • West China School of Stomatology
      • • Department of Oral and Maxillofacial Surgery
      • • Key Laboratory of Oral Biomedical Engineering
      Hua-yang, Sichuan, China
  • 2011
    • West China Hospital of Stomatology
      Hua-yang, Sichuan, China