Letízia Monteiro de Barros

Universidade José do Rosário Vellano – UNIFENAS, Alfenas, Minas Gerais, Brazil

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Publications (17)11.71 Total impact

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    ABSTRACT: Objective: was to analyze the digital and palmar asymmetry of parents and individuals with non syndromic clef lip and/or palate. Method: we analyzed a sample of 303 people divided into two groups, the case with 51 trios consisting of fathers, mothers and children affected by non syndromic cleft palate, since the control group was composed of 50 trios also shaped by individuals unaffected by cleft or other clinical syndromes or conditions of diseases, as well as their fathers and mothers. We made the fingerprints and palm prints of each participant, then calculated the dermatoglyphic patterns (arches, loops and whorls), ridge count the digits and palmar angles (atd, tda, tad). To measure the fluctuating asymmetry (right-left) if the case group was compared to the control using the Mann-Whitney and chi-square tests, and accepted a p value ≤ 0.05. Result: We observed a statistically significant difference between the atd angles of the fathers, and dermatoglyphic patterns of the mothers of those affected by cleft, with a significantly greater number of arches in the case group (p=0.01). With respect to other measures there were no significant differences. Nor was it possible to observe differences in the ridge counts, palmar angles and dermatoglyphic patterns of the son. Conclusion: From these results we can speculate that the mechanisms responsible for deviations in asymmetry of atd angles in fathers and dermatoglyphic patterns in mothers may be associated with the factors responsible for the formation of the CL/P in children.
    IADR General Session 2012; 06/2012
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    ABSTRACT: Hypohidrotic ectodermal dysplasia (HED) is a type of genodermatosis characterized by the abnormal development of sweat glands, teeth, and hair. The most prevalent form of HED is X-linked hypohidrotic ectodermal dysplasia (XLHED), which is associated with mutations in the EDA gene. The aim of this case report was to describe a family with XLHED with emphasis on differences in orofacial features between members. Family members were systematically evaluated to characterize the pattern of inheritance and clinical features. Dental examination included evaluation of agenesis and abnormal teeth structure. The pedigree of the last seven generations of the family was constructed. Clinical examination and medical history revealed five males affected by HED and nine female as heterozygous carriers. The males exhibited the classic phenotype of XLHED, with dental abnormalities, hypohydrosis, and craniofacial dysmorphologies. The heterozygous carriers of the X-linked gene defect principally exhibited dental agenesis of the lateral maxillary incisors. Careful clinical examination, including dental evaluation, is an important way to detect heterozygous carriers of X-linked HED. Heterozygous parents of patients with HED may also show some features of the disorder. The identification of female carriers results in genetic counseling being offered to affected families, as well as providing adequate treatment as necessary and long-term follow-up of these patients.
    Head and Neck Pathology 03/2012;
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    ABSTRACT: Abstract Abstract Objective: To compare the caries experience of adolescents and young adults with cleft lip and/or palate (CL/P) with a non-cleft control group. Design: Thirty CL/P subjects and 30 controls were clinically examined to obtain the DMFT and DMFS indices, gingival bleeding index, plaque index, and active caries lesions. Data concerning oral hygiene, access to fluoridated water, mother's education level, and family income were also collected. Setting: Pro-Smile Center, a reference center for the treatment of facial deformities, Alfenas, Minas Gerais, Brazil. Subjects: Subjects aged 12-21 years with CL/P and without associated syndromes were matched to non-cleft controls by gender, age, living habits, and use of orthodontic devices. Null hypothesis formulated before the beginning of data collection: caries experience in CL/P adolescents and young adults is similar to that observed in the non-cleft controls. Statistical analysis: Data were analyzed using SPSS 17.0 software for Windows Data Editor. CL/P and control groups were compared using the McNemar test, paired t-test and Wilcoxon test. A significance level of 5% was adopted for all tests. Results: There were no significant differences between the groups for oral hygiene and contact with fluoride. Significant differences were found in per capita income, presence of active caries, decayed surfaces, plaque index and gingival bleeding. Conclusions: The caries experience of CL/P subjects was higher than that of the non-cleft individuals. Keywords: dental caries, cleft lip and/or palate, cross-sectional, controlled study.
    The Cleft Palate-Craniofacial Journal 01/2012; · 1.24 Impact Factor
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    ABSTRACT: Abstract Objective: Many studies have demonstrated a high frequency of dental anomalies in patients with cleft lip and/or palate (CL/P). Since dental anomalies may complicate dental treatment, we investigated the prevalence of dental anomalies in a group of Brazilian patients with nonsyndromic CL/P (NSCL/P). Design, Participants, Setting: Retrospective analysis was performed using clinical records of 296 patients with repaired NSCL/P aged between 12 and 30 years without history of tooth extraction and orthodontic treatment. Associations between oral clefts and presence of dental anomalies outside the cleft area were investigated. Results: Dental anomalies were identified in 39.9% of the NSCL/P patients, and tooth agenesis (47.5%), impacted tooth (13.1%) and microdontia (12.7%) were the most common anomalies. CL patients were less affected by dental anomalies compared with CP or CLP patients (p=0.057). Specifically, unilateral CLP patients were significantly more affected by dental anomalies than bilateral CLP patients (p=0.00002), and individuals with unilateral complete CLP (p=0.002) and complete CP (p=0.01) were significantly more affected by tooth agenesis than other cleft types. Ageneses of the premolars (p=0.043) and maxillary lateral incisors (p=0.03) were significantly more frequent in unilateral complete CLP patients. Conclusions: The present study revealed a high frequency of dental anomalies in NSCL/P patients, and further demonstrated that patients with unilateral CLP were frequently more affected by dental anomalies than bilateral CLP. Moreover, our results demonstrate that dental anomalies should be considered during dental treatment planning of individuals affected by NSCL/P. Keywords: Cleft lip and palate; dental anomaly; tooth agenesis; dental treatment.
    The Cleft Palate-Craniofacial Journal 10/2011; · 1.24 Impact Factor
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    ABSTRACT: The genetic diversity of C. albicans oral isolates from 75 healthy schoolchildren from eight schools located in different geographic areas of Piracicaba city, São Paulo state, Brazil, was established using isoenzymes marker (Multilocus Enzyme Electrophoresis - MLEE) and cluster analysis. Patterns of monoclonal and polyclonal oral colonization by C. albicans within and between groups of schoolchildren were identified. However, significant divergence between the observed and the expected genotypic frequencies (Hardy-Weinberg equilibrium test) was not detected in the geographically adjacent groups, suggesting the hypothesis that populations of healthy schoolchildren do not correspond to the selection factor (differential survival) of strains. Two highly polymorphic and distantly genetically related taxa (A and B) were identified within the total population of yeasts, each contained subgroups (A1, A2, A3, A4, B1 and B2) and clusters of moderately related strains (from I to X), suggesting the existence of strains restricted or not to certain groups of geographically limited, healthy students. However, the coexistence of identical strains in healthy schoolchildren from the same school (geographically related) reinforces the hypothesis of oral transmission, where the sources of propagation could be explored. Furthermore, this could also be used in current and retrospective analyses of C. albicans isolated from immunocompetent and immunocompromised people, in order to detect commensal or potentially pathogenic yeast groups, predominantly in candidiasis, and in the development of strategies to prevent transmission or human propagation.
    Brazilian Journal of Microbiology 07/2011; 42(3):1030-46. · 0.76 Impact Factor
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    ABSTRACT: OBJECTIVE: To evaluate the frequency of craniofacial anomalies in patients treated at a Brazilian Reference Center for craniofacial deformities. METHOD: Retrospective epidemiological study evaluating the clinical records of 1,142 patients: 656 (57.4%) male and 486 (42.6%) female, between 1992 and 2008. RESULTS: Among birth defects, non-syndromic cleft lip and/or palate were the most frequent ones (778 cases; 68.1%), followed by single or multiple congenital anomalies without cleft lip and/or palate (240 cases; 21%), recognized syndromes or sequences (56 cases; 5%), syndromes with orofacial cleft as a component (41 cases; 3.5%), and orofacial clefts in association with systemic malformations (27 cases; 2.4%). CONCLUSIONS: Non-syndromic cleft lip and/or palate was the congenital defect most frequently identified, although, isolated anomalies and syndromes involving craniofacial structures were quite frequent. Furthermore, the need for studies to identify the frequency and risk factors associated with craniofacial anomalies in the Brazilian population is emphasized in order to plan comprehensive strategies and integrated actions for the development of preventive programs and treatment.
    Revista Brasileira de Epidemiologia 03/2011; 14(1):151-160.
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    ABSTRACT: Cleft lip and/or palate (CL/P) are the most common congenital anomalies of the face. CL/P are non-syndromic (CL/PNS) in about 70% of subjects. To describe clinical cases of non-syndromic CL/P (CL/PNS) associated with consanguinity, diagnosed at a reference hospital in Minas Gerais, Brazil, and to correlate these alterations with possible risk factors. SERIES AND METHODS: A retrospective study at a reference hospital for craniofacial deformities in Minas Gerais, Brazil from 2006 to 2009 based on data in medical records. Of 246 CL/PNS cases diagnosed and treated at the institution, 15 (6.1%) were CL/PNS with reported first-degree consanguinity; 73.3% occurred in males. Four of 15 patients had complete right cleft palate and lip (CLP), 4 presented complete cleft palate and lip (right and left), 3 had complete unilateral left CLP, 3 had isolated cleft palate, and 1 presented cleft lip only. Among the risk factors, only three mothers reported smoking during pregnancy. CLP (unilateral or bilateral) were more frequent in the group with a history of first-degree consanguinity; males predominated. Among the risk factors, only smoking was observed in three cases.
    Brazilian journal of otorhinolaryngology 02/2011; 77(1):19-23. · 0.55 Impact Factor
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    ABSTRACT: Cleft lip and palate (CL/P) are the most common congenital craniofacial anomalies. To evaluate environmental risk factors for non-syndromic CL/P in a reference care center in Minas Gerais. we carried out a case-controlled study, assessing 100 children with clefts and 100 children without clinical alterations. The analysis dimensions (age, skin color, gender, fissure classification, maternal and paternal age, birth order and interpregnancy interval), obtained from a questionnaire; and later we build a data base and the analyses were carried out by the SPSS 17.0 software. The results were analyzed with the relative risk for each variable, in order to estimate the odds ratio with a 95% confidence interval, followed by a bivariate and multivariate analysis. among 200 children, 54% were males and 46% were females. As far as skin color is concerned most were brown, white and black, respectively. Cleft palates were the most common fissures found (54%), followed by lip cleft (30%) and palate cleft (16%). although with a limited sample, we noticed an association between maternal age and an increased risk for cleft lip and palate; however, paternal age, pregnancy order and interpregnancy interval were not significant.
    Brazilian journal of otorhinolaryngology 02/2010; 76(1):107-12. · 0.55 Impact Factor
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    Brazilian Journal of Otorhinolaryngology (impresso). 01/2010; 76(1).
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    ABSTRACT: The aim of this study was to investigate the oral colonization profile of Candida albicans strains isolated from diabetic patients and their non-diabetic consorts. In addition hydrolytic enzyme activity of these isolates was analysed. The genetic diversity of C. albicans oral isolates from 52 couples was established using isoenzyme marker and cluster analysis. Hydrolytic enzyme characteristics, namely secreted aspartyl proteinases (SAPs) and phospholipases (PLs) were also analysed. Simultaneous colonization by C. albicans was observed in the consorts of 12 couples (23.1%). Patterns of monoclonal and polyclonal oral colonization by C. albicans strains were identified and the coexistence of identical or highly related strains was observed in both members of eight couples. The genetic diversity observed in the total yeast population revealed four large, genetically distinct groups (A to D) and the coexistence of strains in couples or consorts conjugally unrelated. SAP and PL activity was observed in the majority of C. albicans isolates without any association to particular strain, strain clusters (highly related isolates), or clinical characteristics of the consorts (diabetic, non-diabetic, and gender). Possible sources of transmission and oral propagation of groups (clusters) of strains of C. albicans can occur between diabetic and non-diabetic consorts. A conjugal genotypic identity exists in most C. albicans-positive couples, that is, both consorts share identical or highly related strains; however, this identity is not couple-specific as seen by the coexistence of clusters in couples and unrelated consorts.
    Oral Microbiology and Immunology 12/2009; 24(6):437-50. · 2.81 Impact Factor
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    ABSTRACT: Cleft lip and palate (CL/P) are the most common congenital anomalies of the craniofacial region. to evaluate the surgical techniques used in CL/P treatment in a craniofacial deformities ward, in Minas Gerais. In this retrospective study, carried out between 2002 and 2007, we studied 109 individuals with non-syndromic CL/P submitted to treatment. The aspects analyzed (personal identification, classification of CL/P and surgical treatment performed) were obtained from patient charts, and then we built a database and ran statistical analyses through the SPSS 13.0 software. Followed by descriptive analysis of the surgical procedures depending on the type of CL/P found. Among the 109 patients, 65.1% were males and 34.8% females. We found that 45% of patients had cleft lip and palate, 37.6% cleft lip only and 17.4% cleft palate only. The surgical techniques employed were predominantly those from Millard and Spina for cheiloplasty, McComb for rhinoplasty and, Veau and Van Langenbeeck for palatoplasty. This study is the first to address treatment procedures for individuals with CL/P in the state of Minas Gerais. For unilateral CL/P we predominantly used the association of McComb, Veau and Millard techniques, respectively, for rhinoplasty, palatoplasty and cheiloplasty, in 76.9% of the patients.
    Brazilian journal of otorhinolaryngology 12/2009; 75(6):839-43. · 0.55 Impact Factor
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    ABSTRACT: Interferon regulatory factor 6 (IRF6) gene has emerged as a potential susceptibility gene for non-syndromic cleft lip and/or palate (NSCL/P) in different populations. The aim of this study was to determine the association of IRF6 rs2235371 and rs642961 polymorphisms with NSCL/P in a Brazilian population. Two hundred and twenty-eight patients affected by NSCL/P and 126 healthy individuals were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. Overall genotype distributions of rs2235371 and rs642961 polymorphisms were as expected by Hardy-Weinberg equilibrium test. The rs2235371 polymorphic genotype GA was identified in 10.1% of the patients with NSCL/P and in 10.3% of the control group, revealing no statistical difference. Similarly, the frequency of rs642961 minor genotypes (GA and AA) was quite similar between control group (28.6%) and NSCL/P group (25.4%), without significant difference. Our findings are consistent with a lack of involvement of IRF6 rs2235371 and rs642961 polymorphisms in the NSCL/P pathogenesis in the Brazilian population.
    Oral Diseases 09/2009; 16(2):193-7. · 2.38 Impact Factor
  • Brazilian Journal of Otorhinolaryngology (impresso). 01/2009; 75(6).
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    ABSTRACT: Objective: To evaluate the knowledge of laymen, undergraduate dental students and dentists of Human Tooth Bank (HTB). Methods: Observational study with non-probabilistic sample. Onehundred-and-fifty subjects (50 dentists, 50 undergraduate dental students and 50 laymen) were interviewed about their knowledge of donation of organs, valuing of the tooth as an organ, HTB, the use and origin of teeth used in dental schools and acceptance of biological restorations. Results: As much as 97.6% of the interviewees were favorable to organ donation, but only 48% declared themselves as organ donors. The tooth was considered an organ by 94% of the dentists, 90% of the students and 54% of the laymen. During the graduation course, 90% of the dentists and 86% of the undergraduate students confirmed the use of human teeth obtained from dental offices or cemeteries. For improvement of research and teaching purposes, 94% of the students and dentists considered important the implementation of HTBs; however, only 2% of the students, 6% of the laymen and 28% of the dentists declared being familiar with a HTB. Most of the interviewees (90%) were favorable to the donation of teeth to HTBs, but only 44% of them would accept a biological restoration, alleging "repulsion" and the existence of alternative restorative materials. Conclusion: Most interviewed individuals were favorable to tooth donation to a HTB, but ignored its existence as well as the ethical and biosecurity guidelines regulating the maintenance of private tooth collections.
    Pesquisa Brasileira em Odontopediatria e Clínica Integrada 01/2009; 9(1):101-106.
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    ABSTRACT: Mucosal surfaces are the primary oral reservoirs of Candida species, but these species can also be found in subgingival biofilm. The present study investigated the genetic diversity and production of exoenzymes of C. albicans and C. dubliniensis isolated from the oral cavity of systemically healthy patients with periodontitis. Fifty-three patients were analysed. Samples were collected from three oral cavity sites (periodontal pocket, gingival sulci and oral mucosa), plated and, after isolation, suspect strains of C. albicans and C. dubliniensis were identified by PCR. The genetic diversity of the isolates was evaluated by RAPD and the activities of the secreted aspartyl proteinases and phospholipases were evaluated by the agar plate method. Twenty-one patients showed positive results for Candida spp. There were no statistically significant differences between genders, or between sites. C. albicans was the most frequently found specie, while C. dubliniensis was isolated from the periodontal pocket of only one patient. Sixteen genotypes were detected among the C. albicans isolates, and one among the C. dubliniensis isolates. The similarity coefficient (S(SM)) values among the C. albicans genotypes ranged from 0.684 to 1.0 with an average of 0.905+/-0.074. All isolates produced high levels of Saps and most of them produced high levels of phospholipases. No relationship was found between the genotypes and the pattern of enzymatic production. There was no association between specific genotypes and their site of isolation. The results of the present study suggest that genetically homogeneous strains of C. albicans are present in the oral cavity of patients with periodontitis and that these strains are capable of producing high levels of exoenzyme.
    Archives of oral biology 08/2008; 53(12):1172-8. · 1.65 Impact Factor
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    Arquivos Brasileiros De Oftalmologia - ARQ BRAS OFTALMOL. 01/2008; 71(6).
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    ABSTRACT: To evaluate the biopsychosocial profile of patients with anophthalmia, with emphasis on the psychological and functional impact of eye loss and the social reintegration of this population. Prospective analyses of 84 patients (50 males and 34 females), rehabilitated or in the rehabilitation process with ocular prostheses were interviewed by means of a questionnaire with dimensions involving the etiology of the ocular defect, degree of adaptation to the ocular prosthesis, and impact on professional, family and social activities. The right eye was affected in 45.2% of the patients, the left eye in 51.2%, and the rest of the patients had bilateral anophthalmia. Difficulty in adapting to monocular vision was reported by 47.6% of the patients. The main causes of anophthalmia in males were eye injuries due to accidents (54%), and, in females, acquired diseases (38.2%). For the total studied population, the eye loss occurred at a mean of 20.5 +/- 18.41 years, and the elapsed time until the first rehabilitation with ocular prosthesis was of 8.6 +/- 13.10 years. Most patients (66.1%) reported satisfaction and good adaptation to the prosthesis. Feelings of sadness, shame and shyness were frequently reported. Anophtalmic patients often exhibit psychic and/or functional disorders which hinder their social, professional and family readaptation, and this is aggravated by both economic factors and lack of public services that provide rehabilitative treatment. Public information campaigns could also be useful to prevent causes that lead to ocular loss.
    Arquivos brasileiros de oftalmologia 71(6):855-9.

Publication Stats

41 Citations
11.71 Total Impact Points

Institutions

  • 2012
    • Universidade José do Rosário Vellano – UNIFENAS
      Alfenas, Minas Gerais, Brazil
  • 2011–2012
    • University of Campinas
      • Departamento de Diagnóstico Oral
      Conceição de Campinas, São Paulo, Brazil
  • 2010
    • Universidade Estadual de Montes Claros
      MOC, Minas Gerais, Brazil
  • 2009
    • Universidade Federal de Alfenas
      Alfenas, Minas Gerais, Brazil