Denise Pontes Raldi

São Paulo State University, San Paulo, São Paulo, Brazil

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Publications (23)7.52 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Objective: This study sought to assess if discoloration of tooth structures occurs after photodynamic therapy (PDT) and to determine the efficacy of a protocol to remove the photosensitizers. Background data: PDT has been used in root canal treatment to enhance cleaning and disinfection of the root canal system. PDT uses a low power laser in association with a dye as a photosensitizer. Photosensitizers can induce staining of the dental structures, resulting in an unaesthetic appearance. Methods: Forty teeth were randomly divided into four groups according to the photosensitizer used and pre-irradiation time: 0.01% methylene blue for 5 min (MB5); 0.01% methylene blue for 10 min (MB10); 0.01% toluidine blue for 5 min (TB5); and 0.01% toluidine blue for 10 min (TB10). Specimens were irradiated with a 660 nm diode laser with a 300 μm diameter optical fiber, at 40 mW power setting for 3 min. Immediately after, the photosensitizers were removed with Endo-PTC cream+2.5% sodium hypochlorite (NaOCl). The shade was measured by a Vita Easyshade spectrophotometer based on the CIELAB color system (L*a*b* values) at three different experimental times: before PDT (T0), immediately after PDT (T1), and after removal of the photosensitizer (T2). Results: The results showed a decrease in the averages of the L*a*b* coordinate values after PDT (T1) in all the groups, when compared with the number at T0, with a significant statistical difference in group MB10. After photosensitizer removal (T2), all the values of the coordinates increased with significant statistical differences (p<0.05) between T1 and T2 in L* and a*. Conclusions: It can be concluded that both methylene blue and toluidine blue dyes cause tooth discoloration, and that Endo-PTC cream associated with 2.5% NaOCl effectively remove these dyes, regardless of the pre-irradiation time used for PDT.
    Photomedicine and laser surgery. 07/2014;
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    ABSTRACT: This study sought to assess if the final rinse protocol interferes with the smear layer removal in the apical area of curved canals. Sixty-four extracted human mandibular molars with curved mesial roots were instrumented with rotary files and divided into six experimental groups for final rinse: 1EDTA (syringe irrigation with 1 mL of 17% ethylenediaminetetraacetic acid (EDTA) ), 5EDTA (syringe irrigation with 5 mL of 17% EDTA), 1EDTA-P (syringe irrigation with 1 mL of 17% EDTA + pumping with gutta-percha point), 5EDTA-P (syringe irrigation with 5 mL of 17% EDTA + pumping with gutta-percha point), 1EDTA-EA (syringe irrigation with 1 mL of 17% EDTA + EndoActivator) and 5EDTA-EA (syringe irrigation with 5 mL of 17% EDTA + EndoActivator). Final rinsing was carried out over 3 min. The specimens were split lengthwise and observed under a scanning electron microscope using a score criterion. Comparison among the groups showed statistically significant difference only between the 5EDTA-EA group and the other groups (Kruskal-Wallis and Dunn's post-hoc tests, P < 0.05). The combination of 5 mL of 17% EDTA and 3 mL of 2.5% sodium hypochlorite (NaOCl) with the EndoActivator removed smear layer from the apical area of curved root canals more effectively than the other protocols used.
    Australian Endodontic Journal 09/2013; · 0.50 Impact Factor
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    ABSTRACT: Objective: the aim of this study was to evaluate by radiographic and histologic analyses the effects of high power diode laser and topical application of basic fibroblast growth factor (bFGF) on tissue repair of delayed replanted rat teeth. Method: right central incisors of 50 male Wistar rats were used. After the extraction, the specimens were assigned to five groups (n=10):C+ and C- were the control groups-with no treatment and they were respectively submitted to immediate and delayed replantation (DR) 60 minutes after the extraction. The other groups received root surface treatment prior the DR as follow: DL–root surfaces were irradiated with high power diode laser (810 nm, 1.5 W, 30 seconds); DL/FGF– root surfaces were irradiated with high power diode laser and 50 μg FGF-2 gel (2%) was applied on the alveolar wound; FGF – FGF-2 gel was applied on the root surfaces and on the alveolar wound. The specimens were digitally radiographed and processed for histologic analysis to determine the average root resorption areas and to evaluate the tissue repair. Result: the percentage of root resorption was significantly (p <0.05) lower in groups C+ (1.03), DL (1.16) and DL/FGF (0.99) when compared to the groups C- (3.83) and FGF (3.09). Areas of inflammatory and replacement resorptions and ankylosis were more frequent in histological sections of the C- group and on the FGF group. Conclusion: root surface treatment with a high power diode laser associated or not with b FGB reduced the occurrence of external root resorption and topical application of b FGB did not favor significantly the tissue repair.
    IADR/AADR/CADR General Session and Exhibition 2013; 03/2013
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    ABSTRACT: Abstract -  Background aim:  The aim of this study was to evaluate, by radiographic and histomorphometric analyses, the effects of high-power diode laser irradiation on the root surfaces of delayed replanted rat teeth. Material and methods:  Maxillary right incisors were extracted from 60 Wistar rats and kept dry for 60 min. Subsequently, the root canals were prepared and filled with calcium hydroxide paste. According to the root surface treatment before the replantation, the teeth were assigned to four groups (n = 15): G1 (negative control) - no root surface treatment; G2 (positive control) - treated with 2% sodium fluoride solution; G3 - irradiated with a high-power diode laser (810 nm, continuous mode, 1.0 W, 30 s); and G4 - irradiated with a diode laser using the same parameters as those used for G3 but in pulsed mode. The rats were euthanized after 15, 30, and 60 days of replantation. The specimens were digitally radiographed and processed for histomorphometric analysis to determine the average root resorption areas and to evaluate the histological events. Results:  The percentage of root resorption was in the following order: G1 > G2 > G4 > G3. Both histomorphometric and radiographic analyses showed significantly lower means (P < 0.05) of the occurrence of root resorption in the irradiated groups (G3 and G4) when compared to the control groups (G1 and G2). Replacement resorption and ankylosis were observed in histological sections only after 30 and 60 days; however, such events were not observed in G3. Conclusion:  Root surface treatments with high-powered diode laser irradiation prior to delayed replantation reduced the occurrence of external root resorption compared to no treatment or sodium fluoride treatment at up to 60 days.
    Dental Traumatology 01/2012; · 1.00 Impact Factor
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    ABSTRACT: Photodynamic therapy (PDT) is a new technique introduced in endodontics that combines the action of a photosensitizer (dye) and a low intensity light source. Currently, there are no PDT studies evaluating the microbial disinfection of root canals in order to compare the effects of light delivery systems in the photosensitizer activation. The aim of this study was to evaluate the PDT effectiveness in reducing Enterococcus faecalis, with and without the aid of an intracanal optical fiber. Extracted single-rooted teeth were selected, instrumented, inoculated with E. faecalis and divided into six groups: one control group (untreated), one conventionally-treated group (1% NaOCl irrigation) and four PDT-treated groups. Irradiation (diode laser) was performed with (OF) or without an intracanal optical fiber (NOF) using two different irradiation times: 1 min and 30 sec (IT(90)) or 3 min (IT(180)). Samples were collected before and after testing procedures and CFU/mL was determined. The greatest reduction of E. faecalis (99.99%) was achieved with irrigation with 1% NaOCl. PDT also significantly reduced E. faecalis in the following decreasing order: OF/IT(180), NOF/IT(180), OF/IT(90) and NOF/IT(90), with no significant statistical difference among the groups. These results suggest that PDT was effective against E. faecalis, regardless of the use of an intracanal optical fiber.
    Photomedicine and laser surgery 07/2011; 29(12):803-8. · 1.76 Impact Factor
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    ABSTRACT: The disadvantage of photodynamic therapy (PDT) is in the photosensitizing agents that may stain the tooth structure. There is no register of PDT studies evaluating protocols to minimize that concern. The present study evaluated the efficiency of chemical adjuncts in methylene blue dye (MB) removal after PDT. Forty single-rooted teeth, after root canal preparation, were filled with 0.01% MB for 5  min, and irradiated with diode laser 660  nm at 40  mW for 240  sec (total energy 9.6  J). The specimens were divided into four groups (n = 10), according to the chemical adjuncts used for dye removal: (a) 2.5% sodium hypochlorite (NaOCl); (b) 2.5% NaOCl + Endo-PTC cream; (c) 70% ethyl alcohol and (d) saline (control). The crowns were sectioned and fixed in a device. Photographs were taken before the PDT (T0), immediately after (T1) and upon dye removal (T2). The chromatic alterations were evaluated using Adobe Photoshop and K values were determined in four fixed points of each crown. K values (dental staining) increased in all groups when comparing T0 and T1. The effectiveness of the tested adjuncts was, in decreasing order: G1 (-3.11) > G2 (-2.97) > G3 (-1.28) > G4 (-1.19), not observing significant statistical differences (p < 0.05) between G1 and G2 and also between G3 and G4. Protocols to remove photosensitizing dyes should be applied after PDT in order to minimize dental stain. The protocols tested in this study by using 2.5% NaOCl, associated or not with Endo-PTC cream, were effective in avoiding tooth staining caused by MB during PDT.
    Photomedicine and laser surgery 06/2011; 29(8):559-63. · 1.76 Impact Factor
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    ABSTRACT: Nitric oxide (NO) has been considered a key molecule in inflammation. The aim of this study was to evaluate the effect of treatment with L-NAME and sodium nitroprussiate, substances that inhibit and release NO, respectively, on tissue tolerance to endodontic irrigants. The vital dye exudation method was used in a rat subcutaneous tissue model. Injections of 2% Evans blue were administered intravenously into the dorsal penial vein of 14 male rats (200-300 g). The NO inhibitor and donor substances were injected into the subcutaneous tissue in the dorsal region, forming two groups of animals: G1 was inoculated with L-NAME and G2 with sodium nitroprussiate. Both groups received injections of the test endodontic irrigants: acetic acid, 15% citric acid, 17% EDTA-T and saline (control). After 30 min, analysis of the extravasated dye was performed by light absorption spectrophotometry (620 nm). There was statistically significant difference (p<0.05) between groups 1 and 2 for all irrigants. L-NAME produced a less intense inflammatory reaction and nitroprussiate intensified this process. Independently of the administration of NO inhibitors and donors, EDTA-T produced the highest irritating potential in vital tissue among the tested irrigating solutions.
    Journal of applied oral science: revista FOB 04/2011; 19(2):113-7. · 0.39 Impact Factor
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    ABSTRACT: Objectives: The aim of this study was to evaluated by clinical and radiographic analysis the effects of high power diode laser irradiation in root surfaces prior delayed replantion tooth. Methods: Forty rat maxillary right incisors (Rattus novergicus, Wistar) were extracted and maintained in a dry environment for 60 min. After, the pulp was extirpated and the root canals were filled with calcium hydroxide past. The specimens were divided into 4 groups (n=5), according to the treatment applied in root surface: G1- untreated treatment, G2 - immersed in 20 mL of 2% acidulated phosphate sodium, G3 - irradiation of the root surface with high power diode laser (810 nm, continuous mode, 1.0 W, 30 s) and G4 - irradiation with diode laser with the same parameters used in group 3, although in pulsed mode. The teeth were replanted and after periods of 15 and 30 days the animals were anesthetized for clinical analysis. Later on, the animals were killed for radiographic analysis purpose, which has been developed by calculating the number of reabsorption areas in the root surface and, also, by determining the radiographic density of the area. Results: The worst results were observed in the group that did not receive treatment of root surface (G1) and the best ones in the groups irradiated by diode laser (G3 and G4). There was statistically significant difference among the irradiated groups and the control group regarding the clinical aspects and the number of reabsorption areas, in both experimental periods (P
    IADR General Session 2010; 07/2010
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    ABSTRACT: The present study evaluated the fibroblast attachment and the morphologic changes of simulated cervical root resorptions after irradiation with high-power lasers and the use of mineral trioxide aggregate (MTA). Standardized cavities were done on the coronal third of 40 single-rooted teeth and assigned into 4 groups according to the treatment applied: (1) untreated control, (2) use of MTA, (3) irradiation with Er:YAG laser (42 mJ, 10 Hz, 10 s), and (4) irradiation with high-power diode laser (1 W, 10 s). Fragments were prepared for SEM analysis: 6 samples of each group were plated with human gingival fibroblasts and 4 samples were used for ultrastructural analysis. The cells' attachment was in decreasing order: G3 > G4 > G1> G2, with statistical differences among all groups (P < .05). The samples irradiated with Er:YAG exhibited roughness on the dentinal surface, no smear layer, and open dentinal tubules; those irradiated with diode laser exhibited a smooth surface, more smear layer, and closed dentinal tubules; and the control group showed high amount of smear layer and closed dentin tubules. Irradiation with Er:YAG and diode lasers caused morphologic changes on the dentinal surfaces of simulated resorptions that favored cells' adhesion. MTA showed lower biocompatibility than irradiated groups but allowed cells' adhesion.
    Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology 12/2009; 109(2):e154-61. · 1.50 Impact Factor
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    ABSTRACT: Three clinical cases involving teeth with open apices and apical periodontitis were treated using different protocols. The first case was managed with intracanal calcium hydroxide paste for 12 months before obturation with gutta-percha and sealer. In the second case, an apical plug of mineral trioxide aggregate (MTA) was used before obturation with gutta-percha and sealer and treatment was completed during 2 appointments. In the third case, the tooth, which had a divergent root canal system, was completely obturated with MTA and treatment was also completed over 2 appointments. In all 3 cases, signs of bone healing were observed after treatment.
    Journal (Canadian Dental Association) 10/2009; 75(8):591-6. · 0.62 Impact Factor
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    ABSTRACT: Objectives: the aim of this study was to evaluate in vitro the photodynamic therapy effectiveness on the reduction of Enterococcus faecalis using or not intracanal optical fiber associated with different parameters of irradiation. Methods: sixty human single-root teeth were selected. After instrumentation the root canals were inoculated with E. Faecalis and randomly divided into six groups (n=10). Four groups received a solution of 0.01% methylene blue for 5 min followed by irradiation using a diode laser at a wavelength of 680 nm, than according to the employed parameters they were divided in: G1- irradiated with 216 micron intracanal optical fiber, at a power output (P) of 90mW and irradiation time (t) of 1min 30s;G2- irradiated with 600 micron hand peace placed on the root canal orifice (without intracanal optical fiber), P=100mW and t=1min 30s, G3- irradiated with intracanal optical fiber, P= 90mW and t=3min; G4- irradiated as the same way as the group G2 (without optical fiber), P= 100mW and t=3min. The two other groups served as control: G5 (positive control)- treated with 1% sodium hypochlorite solution for 15min and G6 (negative control)- did not undergo any intervention. Bacterial samples were taken before and after irradiation. In each of the samples, the number of colony-forming units (CFU) was counted. Results: all treatments resulted in reduction of microrganisms and the mean decrease in CFU was: G1- 99,48%, G2-99,41%, G3-99,65 %, G4-99,64% and G5-100%, with no significant difference between the irradiated groups (p>0.05). Conclusions: the photodynamic effectiveness with the irradiated parameters employed in this study was independent of the use of intracanal optical fiber.
    IADR General Session 2009; 04/2009
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    ABSTRACT: During endodontic therapy, cleaning of root canals is performed using endodontic files and auxiliary chemical substances, and it is important that the endodontist be familiar with the instruments used in daily practice. This study evaluated, under scanning electron microscopy (SEM), the quality of the surface finishing of unused rotary endodontic instruments. Fifty sizes 20, 25 and 30 rotary files from different commercial brands (ProFile, Protaper, Race, Hero and K3 Endo) were removed directly from their packages and had their final 3 mm examined with a scanning electron microscope at x190 magnification with no previous preparation. The images were evaluated by 3 skillful, calibrated, blinded observers according to the following criteria: cutting edge, debris, grooves, microcavities, tip shape, tip position, scraping and transition angle. Data were recorded in worksheets designed for the study. Irregular edges were observed in 50-100% of the files. Except for ProFile, all commercial brands presented surface debris in 100% of samples. Only Race files showed no grooves or microcavities. K3 Endo files presented the best tip centralization. Excetp for ProTaper files, all commercial brands presented blunt-cutting edges in 100% of samples. All types of files presented surface scraping. K3 Endo files and Protaper had a high percentage of transition angle. Based on the evaluation criteria used in the present study, most samples presented a minimum of 2 alterations and a maximum of 7 alterations per instrument. Under the tested conditions, the quality of the surface finishing of the examined instruments was not as expected, given that no instrument was free of imperfections and most of them presented at least 2 and up to 7 types of surface defects. These results suggest that the manufacturing process and the packaging conditions of rotary endodontic instruments are far from ideal.
    Brazilian dental journal 02/2008; 19(2):109-13.
  • Rev. odonto ciênc. 01/2008; 23(2):182-186.
  • Revista Odonto Ciência (Journal of Dental Science). 01/2008; 23(2).
  • 01/2005;
  • Rev ABENO. 01/2003; 3(1):15-23.
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    Denise Pontes Raldi, José Luiz Lage-Marques
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    ABSTRACT: The purpose of this study was to evaluate in vitro the effects of different associations between irrigating so- lutions (EDTA-T and citric acid), intracanal medicament (NDP), and Er:YAG laser irradiation on dentin permeability. Fifty-one extracted single-rooted teeth were instrumented and divided into seven groups. Groups GI and GII had final irrigation with a demineralizing solution only (EDTA-T and citric acid, respectively). Groups GIII and GIV had final irri- gation with EDTA-T and citric acid, respectively, plus an association of irrigating solution and Er:YAG laser. Groups GV and GVI had final irrigation with EDTA-T and citric acid, respectively, plus an association of intracanal medication and Er:YAG laser. Group GVII (control group) had final irrigation with distilled water. All root canals were filled with NDP associated with rhodamine B dye. After the experimental period, the samples were transversely cut into six 2.0 mm thick slices for subsequent reading using the ImageLab software. Analysis of the results allowed us to conclu- de that there were statistically significant differences (p < 0.05) between the groups as to the penetration of the dye-in- tracanal medication solution. Groups III and IV presented smaller values of dentinal permeability when compared to the other groups. The best results were obtained with the interaction between a demineralizing irrigating solution and the association of intracanal medicament and laser Er:YAG (groups V and VI). In these groups the observed penetra- tion of the intracanal medicament plus dye solution in the apical third was, on average, 29% greater than in the other groups. DESCRIPTORS: Dentin permeability; Root canal irrigants; Lasers. RESUMO: Este experimento teve como objetivo avaliar in vitro os efeitos da interação entre soluções irrigantes desmineralizadoras (EDTA-T e ácido cítrico), medicação intracanal (NDP) e laser Er:YAG na permeabilidade dentinária. Foram utilizados 51 dentes unirradiculares extraídos que, após o preparo químico-cirúrgico, foram divididos em sete grupos experimentais: grupo sIeI I -irrigação final com solução de EDTA-T e ácido cítrico, respectivamente; grupos III e IV - irrigação final com EDTA-T e ácido cítrico, respectivamente, mais a associação entre solução irrigante e laser Er:YAG; grupo sVeV I -irrigação final com EDTA-T e ácido cítrico, respectivamente, mais a associação entre medicação intracanal e laser Er:YAG, e grupo VII (controle) - irrigação final com água destilada. Os canais radiculares foram preenchidos com o corante rodamina B solubilizado na medicação de uso intracanal NDP. Após o período experimental, as amostras foram cortadas transversalmente para posterior leitura com o software ImageLab. A análise dos resultados permitiu concluir que existiram diferenças estatisticamente significantes (p < 0,05) quanto à penetração da solução corante-medicação intracanal nos diferentes grupos. Os grupos III e IV apresentaram menores valores de permeabilidade dentinária quando comparados aos outros e, finalmente, os melhores resultados foram obtidos quando da interação entre a solução irrigante desmineralizadora e a associação medicação intracanal/laser Er:YAG (grupos V e VI). Nesses, constatou-se que a diferença de penetração da solução corante-medicação intracanal no terço apical foi, em média, 29% maior do que nos demais grupos. DESCRITORES: Permeabilidade da dentina; Irrigantes do canal radicular; Lasers.
    Pesquisa Odontológica Brasileira 01/2003; 17(3):278-285.
  • DP Raldi, JL Lage-Marques
    JOURNAL OF DENTAL RESEARCHJOURNAL OF DENTAL RESEARCH; 01/2002
  • Rev. Assoc. Paul. Cir. Dent. 01/2002; 56(5):350-357.
  • JOURNAL OF DENTAL RESEARCHJOURNAL OF DENTAL RESEARCH; 01/2001