M L Pedro-Botet

Publications (27)75.2 Total impact

• Source

  Available from: rairo-ro.org

  Article: Cytopathogenicity and molecular subtyping of Legionella pneumophila environmental isolates from 17 hospitals.

  M Garcia-Nuñez, M L Pedro-Botet, S Ragull, N Sopena, J Morera, C Rey-Joly, M Sabria
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  ABSTRACT: The cytopathogenicity of 22 Legionella pneumophila isolates from 17 hospitals was determined by assessing the dose of bacteria necessary to produce 50% cytopathic effect (CPED50) in U937 human-derived macrophages. All isolates were able to infect and grow in macrophage-like cells (range log10 CPED50: 2.67-6.73 c.f.u./ml). Five groups were established and related to the serogroup, the number of PFGE patterns coexisting in the same hospital water distribution system, and the possible reporting of hospital-acquired Legionnaires' disease cases. L. pneumophila serogroup 1 isolates had the highest cytopathogenicity (P=0.003). Moreover, a trend to more cytopathogenic groups (groups 1-3) in hospitals with more than one PFGE pattern of L. pneumophila in the water distribution system (60% vs. 17%) and in hospitals reporting cases of hospital-acquired Legionnaires' disease (36.3% vs. 16.6%) was observed. We conclude that the cytopathogenicty of environmental L. pneumophila should be taken into account in evaluating the risk of a contaminated water reservoir in a hospital and hospital acquisition of Legionnaires' disease.
  Epidemiology and Infection 07/2008; 137(2):188-93. · 2.84 Impact Factor
• Article: Sporadic and epidemic community legionellosis: two faces of the same illness.

  N Sopena, L Force, M L Pedro-Botet, P Barrufet, G Sauca, M García-Núñez, G Tolchinsky, J A Capdevila, M Sabrià
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  ABSTRACT: The present study compares the risk factors, presentation and outcome of community-acquired Legionella pneumophila pneumonia in 138 sporadic-case patients (1994-2004) and 113 outbreak-case patients (2002) treated in two hospitals in Catalonia (Spain) since urinary antigen assays were adopted. Univariate and multivariate analysis were performed to compare epidemiological and clinical features, blood chemistry values, radiological findings and outcome of sporadic and epidemic legionnaires' disease. Univariate analysis showed that male sex, chronic lung disease, HIV infection and immunosuppressive therapy prevailed in sporadic cases. Presentation with respiratory symptoms, confusion and blood chemistry alterations, such as hyponatraemia, aspartate aminotransferase and blood urea nitrogen elevation, and partial pressure of oxygen P(O)(2) <7.98 KPa (60 mmHg) were also more frequent in sporadic cases, while headache prevailed in outbreak cases. Sporadic cases had a greater delay in treatment, were more severe and had a worse outcome than epidemic cases. Multivariate analysis showed significant differences in sex, chronic lung disease, HIV infection and headache. The clinical and outcome differences between the two groups may be explained by the detection of milder forms of legionnaires' disease, the earlier treatment and the lower severity of underlying disease in the outbreak cases.
  European Respiratory Journal 01/2007; 29(1):138-42. · 5.89 Impact Factor
• Article: Severe Legionnaires' disease successfully treated with levofloxacin and azithromycin.

  M L Pedro-Botet, A García-Cruz, C Tural, L Mateu, N Sopena, S Roure, C Rey-Joly, M Sabria
  Journal of chemotherapy (Florence, Italy) 11/2006; 18(5):559-61. · 1.08 Impact Factor
• Article: Factors related to persistence of Legionella urinary antigen excretion in patients with legionnaires' disease.

  N Sopena, M Sabrià, M L Pedro-Botet, E Reynaga, M García-Núñez, J Domínguez, L Matas
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  ABSTRACT: The aim of this prospective study was to compare patient characteristics, clinical data, and evolution of Legionella pneumophila pneumonia according to the duration of Legionella urinary antigen excretion. Urine samples from 61 patients with Legionella pneumonia diagnosed by detection of urinary antigen were obtained periodically until urinary antigen could no longer be detected. Cases were divided into two groups based on the duration of urinary antigen excretion: group I (46 patients, <60 days) and group II (15 patients, >or=60 days). Groups were compared for patient characteristics, clinical data, and evolution of pneumonia. Antigen excretion >or=60 days was observed significantly more frequently in immunosuppressed patients ( P=0.001) in whom the time to apyrexia was >72 h ( P=0.002), although only the time to apyrexia remained significant on multivariate analysis ( P=0.006). In conclusion, the duration of Legionella urinary antigen excretion was <60 days in most patients but was longer in immunosuppressed patients with a longer time to defervescence of fever.
  European Journal of Clinical Microbiology 12/2002; 21(12):845-8. · 2.86 Impact Factor
• Article: Presence and chromosomal subtyping of Legionella species in potable water systems in 20 hospitals of Catalonia, Spain.

  M Sabrià, M García-Nuñez, M L Pedro-Botet, N Sopena, J M Gimeno, E Reynaga, J Morera, C Rey-Joly
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  ABSTRACT: To investigate the presence and clonal distribution of Legionella species in the water supply of 20 hospitals in Catalonia, Spain. 20 hospitals in Catalonia, an area of 32,000 km2, located in northeast Spain. Environmental cultures of 186 points of potable water supply and 10 cooling towers were performed for the presence of Legionella species. Following filtration and acid treatment, the samples were seeded in selective MWY (modified Wadowsky Yee)-buffered charcoal yeast extract-alpha agar. All isolates obtained were characterized microbiologically and genotyped by SfiI pulsed-field gel electrophoresis (PFGE). 73 of 196 water samples, representing 17 of the 20 hospitals included in the study, were positive for Legionella pneumophila (serogroups 1, 2-14, or both). The degree of contamination ranged from 200 to 74,250 colony-forming units/L. Twenty-five chromosomal DNA subtypes were detected by PFGE. A single DNA subtype was identified in 10 hospitals, 2 DNA subtypes were observed in 6 hospitals, and 1 hospital exhibited 3 different DNA subtypes. Each hospital had its own Legionella DNA subtype, which was not shared with any other hospitals. Legionella was present in the water of most of the hospitals studied; each such hospital had a unique, dominant chromosomal DNA subtype. The verification of several genomic DNA restriction profiles in such a small geographic area demonstrates the great genetic diversity of Legionella in the aquatic environment.
  Infection Control and Hospital Epidemiology 12/2001; 22(11):673-6. · 3.67 Impact Factor
• Article: Appearance of methicillin-resistant Staphylococcus aureus (MRSA) sensitive to gentamicin in a hospital with a previous endemic distinct MRSA.

  N Sopena, M Garcia-Nuñez, R Prats, M L Pedro-Botet, S Elia, J Nieto, M Sabrià
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  ABSTRACT: Since 1990 a clone of gentamicin and methicillin-resistant Staphylococcus aureus (MRSA) has remained endemic in our hospital, but since January 1996 a gentamicin-sensitive strain has progressively replaced the previous clone. We characterized the phenotypic and molecular pattern of the MRSA strains isolated in our hospital in 1996 and compared prospectively the epidemiological, clinical and evolutionary characteristics of ninety patients infected or colonized by gentamicin-sensitive MRSA (GS-MRSA) (49) and by gentamicin-resistant MRSA (GR-MRSA) (41). Finally we studied the variation of aminoglycoside consumption in our hospital from 1989 to 1996. We observed two antibiotypes (GS-MRSA and GR-MRSA) corresponding to two major chromosomal patterns. Patients with GS-MRSA usually acquired the infection 72 hours after hospital admission. No significant differences were observed in epidemiological characteristics, clinical presentation and evolution between patients with GS-MRSA and GR-MRSA. Since 1989 aminoglycoside intake in our hospital has decreased by 46%.
  European Journal of Epidemiology 02/2001; 17(4):317-21. · 4.71 Impact Factor
• Article: [Stevens-Jhonson syndrome associated with vancomcyn].

  S Santaeugenia, M L Pedro Botet, M Sabat, N Sopena, M Sadria
  Revista espanola de quimioterapia: publicacion oficial de la Sociedad Espanola de Quimioterapia 01/2001; 13(4):425-6. · 0.81 Impact Factor
• Article: Cavitating lung lesion and hemoptysis in a young woman.

  M L Pedro-Botet, A Olazabal, J Astudillo, N Sopena, J M Mòdol, M Sabria
  Clinical Microbiology and Infection 06/2000; 6(5):263-4. · 4.54 Impact Factor
• Article: [Central nervous system involvement in tuberculosis].

  E Reynaga, M L Pedro-Botet, N Sopena, M Sabrià
  Medicina Clínica 05/2000; 114(14):559. · 1.38 Impact Factor
• Article: Prospective study of community-acquired pneumonia of bacterial etiology in adults.

  N Sopena, M Sabrià, M L Pedro-Botet, J M Manterola, L Matas, J Domínguez, J M Modol, P Tudela, V Ausina, M Foz
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  ABSTRACT: The aim of this study was to prospectively analyze the bacterial etiology of community-acquired pneumonia in adults in Spain. From May 1994 to February 1996, 392 episodes of CAP diagnosed in the emergency department of a 600-bed university hospital were studied. An etiological diagnosis based on noninvasive microbiological investigations was achieved in 228 cases (58%); 173 of these diagnoses were definitive and 55 probable. Streptococcus pneumoniae, which caused 23.9% of the episodes, was the predominant pathogen observed, followed by Chlamydia pneumoniae (13.5%) and Legionella pneumophila (12.5%). Other less frequent pathogens found were Haemophilus influenzae (2.3%), Pseudomonas aeruginosa (1.5%), Mycoplasma pneumoniae (1.3%), Coxiella burnetii (1%), Moraxella catarrhalis (2 cases), Nocardia spp. (2 cases), and Staphylococcus aureus (2 cases). Streptococcus pneumoniae was significantly more frequent in patients with underlying disease and/or age > or =60 years (28% vs. 13%, P = 0.002), while Legionella pneumophila was more frequent in patients below 60 years of age and without underlying disease (20% vs. 9%, P = 0.006). Likewise, Streptococcus pneumoniae and Legionella pneumophila were the most frequent etiologies in patients requiring admission to the intensive care unit, occurring in 29% and 26.3% of the patients, respectively. In addition to Streptococcus pneumoniae, other microorganisms such as Chlamydia pneumoniae and Legionella spp. should be seriously considered in adults with community-acquired pneumonia when initiating empiric treatment or ordering rapid diagnostic tests.
  European Journal of Clinical Microbiology 12/1999; 18(12):852-8. · 2.86 Impact Factor
• Source

  Available from: Pere Tudela

  Article: Comparative study of the clinical presentation of Legionella pneumonia and other community-acquired pneumonias.

  N Sopena, M Sabrià-Leal, M L Pedro-Botet, E Padilla, J Dominguez, J Morera, P Tudela
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  ABSTRACT: The aim of this study was to compare the clinical, biological, and radiologic features of presentation in the emergency ward of community-acquired pneumonia (CAP) by Legionella pneumophila (LP) and other community-acquired bacterial pneumonias to help in early diagnosis of CAP by LP. Three hundred ninety-two patients with CAP were studied prospectively in the emergency department of a 600-bed university hospital. Univariate and multivariate analyses were performed to compare epidemiologic and demographic data and clinical, analytical, and radiologic features of presentation in 48 patients with CAP by LP and 125 patients with CAP by other bacterial etiology (68 by Streptococcus pneumoniae, 41 by Chlamydia pneumoniae, 5 by Mycoplasma pneumoniae, 4 by Coxiella burnetii, 3 by Pseudomonas aeruginosa, 2 by Haemophilus influenzae, and 2 by Nocardia species. Univariate analysis showed that CAP by LP was more frequent in middle-aged, male healthy (but alcohol drinking) patients than CAP by other etiology. Moreover, the lack of response to previous beta-lactamic drugs, headache, diarrhea, severe hyponatremia, and elevation in serum creatine kinase (CK) levels on presentation were more frequent in CAP by LP, while cough, expectoration, and thoracic pain were more frequent in CAP by other bacterial etiology. However, multivariate analysis only confirmed these differences with respect to lack of underlying disease, diarrhea, and elevation in the CK level. We conclude that detailed analysis of features of presentation of CAP allows suspicion of Legionnaire's disease in the emergency department. The initiation of antibiotic treatment, including a macrolide, and the performance of rapid diagnostic techniques are mandatory in these cases.
  Chest 06/1998; 113(5):1195-200. · 5.25 Impact Factor
• Source

  Available from: oxfordjournals.org

  Article: Role of immunosuppression in the evolution of Legionnaires' disease.

  M L Pedro-Botet, M Sabria-Leal, N Sopena, J M Manterola, J Morera, R Blavia, E Padilla, L Matas, J M Gimeno
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  ABSTRACT: The clinical course of legionella pneumonia in immunosuppressed patients is uncertain. This study was undertaken to determine the clinical evolution of legionellosis on the basis of the immune state and to establish the variables associated with death directly related to legionellosis. The study included 78 patients: 28 with chronic disease who had received immunosuppressive treatment (group 1), 24 with chronic disease without immunosuppressive treatment (group 2), and 26 controls. Inclusion criteria were the occurrence of nosocomially acquired pneumonia, Legionella pneumophila infection, and erythromycin therapy that was initiated within 72 hours following diagnosis. Respiratory and extrarespiratory complications were observed more frequently in groups 1 and 2. Bilateral radiological involvement was most frequent in group 1, and recurrence of legionella pneumonia was observed exclusively in group 1. None of these variables achieved statistical significance. The global mortality of the series was 11.5% (17.9%, 12.5%, and 3.8% in groups 1, 2, and 3, respectively). Variables statistically related to mortality were acute renal failure, shock, and need for mechanical ventilation. Although many of the variables analyzed lacked statistical significance, a trend was seen between complications and basal immunosuppression, as previously suggested.
  Clinical Infectious Diseases 02/1998; 26(1):14-9. · 9.15 Impact Factor
• Article: [Impact of control measures on the course of an outbreak of methicillin-resistant Staphylococcus aureus].

  N Sopena, M Sabrià, M L Pedro-Botet, M Giménez, M Esteve, M Caraballo, E Mesalles
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  ABSTRACT: Outbreaks of nosocomial infection by methicillin resistent Staphylococcus aureus (MRSA) are a problem in many hospitals with the control measures to be adopted being controversial. An outbreak of MRSA in a 550-bed university hospital is herein described and the impact of the adopted control measures on the evolution of the epidemic in the general hospitalization area (GHA) was analyzed. The adopted control measures in the GHA were: microbiologic surveillance, cutaneous isolation measures, treatment of nasal carrier, and the early discharge of the cases. Hand washing was reinforced and a study of carriers was carried out on detection of sporadic cases (not related to the ICU). A molecular study of 70 strains of MRSA was performed with analysis of total plasmids, plasmid restriction pattern and chromosomic DNA analysis by pulsed field gel electrophoresis (PFGE). From December 1990 to December 1993, 273 cases of MRSA were reported. One hundred seventy-two cases originated in the ICU and 101 cases in the GHA (sporadic cases). The incidence of MRSA in 1991-1993 was 13.6, 14.3, and 6.6% in the ICU and 0.17, 0.36, and 0.15% in the GHA, respectively. Molecular study of MRSA isolates (1991 and 1992) demonstrated two plasmid and two chromosomic patterns. The latter had a similarity coefficient > 0.90, probably belonging to the same "clone". Despite the control measures adopted in the GHA the outbreak of MRSA originated in the ICU thereafter extending to the GHA. The rates of colonization detected, however, remained stable during the 3 years studied. On the other hand, the observation of a single "clone", responsible for the epidemic, suggest that most of the sporadic cases were autoctonous and due to failure in fulfillment of the established norms.
  Medicina Clínica 04/1997; 108(11):401-4. · 1.38 Impact Factor
• Article: [Invasive Streptococcus agalactiae infections at a general university hospital over a 10-year period].

  M Giménez, N Sopena, B Viñado, P J Cardona, M L Pedro-Botet, W Coroleu, J Arnal
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  ABSTRACT: Streptococcus agalactiae is a known causal agent of neonatal meningitis, sepsis and puerperal infections. The incidence of invasive infections caused by Streptococcus agalactiae has increased in recent years in non gestating adults: in the elderly, patients receiving prolonged steroid treatment or those with chronic immunosuppressive diseases. The clinical and epidemiological characteristics and risk factors associated to invasive infections caused by S. agalactiae were analyzed. A retrospective study was undertaken in patients with invasive disease by S. agalactiae attended in the Hospital Universitari Germans Trias i Pujol in Badalona (Barcelona), Spain, from 1983 to 1993. S. agalactiae was isolated in 51 patients including 13 (25%) neonates. Three patients presented invasive puerperal infection. Thirty-five adult patients with a mean age of 62 years presented invasive disease. Infection involved bacteremia in 26 (74.2%) patients. S. agalactiae was isolated in the ascitic fluid of 4 patients with liver cirrhosis with spontaneous bacterial peritonitis (one with bacteriemia) and in the peritoneal exudate of two patients with peritonitis secondary to intestinal perforation. Of 5 patients with septic arthritis, 3 involved bacteremia. Two patients presented empyema by S. agalactiae. Mortality was 28%, being directly related with infection in 4 cases (7.8%). Without taking pregnant women into account, 68% of the cases of invasive infections by S. agalactiae were observed in adults with associated base disease, with liver cirrhosis, neoplasms and diabetes mellitus being the most frequent. Advanced age was also found to be an important predisposing factor.
  Enfermedades Infecciosas y Microbiología Clínica 06/1996; 14(5):300-3. · 1.49 Impact Factor
• Source

  Available from: ersj.org.uk

  Article: Nosocomial and community-acquired Legionella pneumonia: clinical comparative analysis.

  M L Pedro-Botet, M Sabria-Leal, M Haro, C Rubio, G Gimenez, N Sopena, J Tor
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  ABSTRACT: Previous reports have suggested that nosocomial and community Legionella pneumonia cases are similar. However, community and hospital characteristics, such as aquatic environment, antibiotic pressure (usage) and populations, are quite different, leading to the suspicion that Legionella infection may differ in the two settings. Univariate and multivariate analyses were performed to compare demographic data, risk factors, clinical, radiological and outcome data between 125 nosocomial and 33 community-acquired cases of Legionella pneumophila infection. Patients in the nosocomially acquired Legionella pneumonia (NALP) group were older than those in the community-acquired Legionella pneumonia (CALP) group. Univariate analysis showed that smoking habit, cough, thoracic pain, and extrapulmonary manifestations were more prevalent in the CALP group, whilst chronic lung disease and cancer were more prevalent in the NALP group. Moreover, patients in the NALP group were more likely to have received oxygen and corticosteroid therapy and also to have altered creatinine values than patients in the CALP group, whilst more patients in the latter group had altered alanine amino-transferase values. However, multivariate analysis failed to confirm most of these differences. Smoking habit and blood creatinine levels were the only variables remaining significant. In conclusion, demographic, clinical, laboratory, radiological and outcome data in nosocomial and community-acquired Legionella pneumonia are quite similar.
  European Respiratory Journal 12/1995; 8(11):1929-33. · 5.89 Impact Factor
• Article: [Invasive infections caused by Streptococcus pyogenes. Changes in their clinical characteristics, new physiopathologic aspects, and current therapeutic approach].

  P Torrabadella, M L Pedro-Botet, J Xirgu, S Armengol, R Catalán
  Enfermedades Infecciosas y Microbiología Clínica 01/1995; 12(10):505-10. · 1.49 Impact Factor
• Article: Molecular epidemiology for local outbreaks of methicillin resistant Staphylococcus aureus (MRSA). The need for several methods.

  M Sabria-Leal, V H Morthland, M L Pedro-Botet, N Sopena, M Gimenez-Perez, M L Branchini, M A Pfaller
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  ABSTRACT: Subtyping isolates may be useful for epidemiological studies of methicillin-resistant-Staphylococcus aureus (MRSA) outbreaks. Among subtyping methods, DNA-based techniques have been applied very effectively for this purpose. An outbreak of MRSA infections took place in one hospital in Barcelona early during 1991. From the beginning of the outbreak to December 92, 70 MRSA isolates from different patients and sources were collected. All strains were evaluated by restriction endonuclease analysis of plasmid DNA (REAP) and macrorestriction endonuclease analysis of genomic DNA using Sma I and pulsed-field-gel-electrophoresis (PFGE). Plasmid screening and REAP using Hind III demonstrated two plasmid subtypes: subtype A showing a large plasmid, and subtype B showing the same large plasmid plus a smaller one. Subtypes A and B corresponded to the more recent and older isolates, respectively, suggesting the loss of the small plasmid during the epidemic. PFGE using Sma I displayed two closely related profiles (PFGE subtype A and A'; CS = 0.90). These subtypes were different from those subtypes exhibited from 4 methicillin-susceptible-Staphylococcus aureus (MSSA) isolates from the same hospital and from 2 epidemiologically unrelated MRSA isolates. Almost all isolates showing PFGE subtype A preceded those isolates showing PFGE subtype A'. This fact and the similarity between both subtypes suggested minor chromosomal DNA rearrangement during the outbreak from a unique strain. While PFGE using Sma I is a useful tool in evaluation of clonal dissemination, our data suggest epidemic or local outbreaks may need several methods to best delineate the source and spread of MRSA strains. The reproducibility and discriminatory power of REAP makes it a useful adjunct in this context.
  European Journal of Epidemiology 07/1994; 10(3):325-30. · 4.71 Impact Factor
• Article: A distinctive cytologic pattern for diagnosing tuberculous lymphadenitis in AIDS.

  M Llatjos, J Romeu, B Clotet, G Sirera, J M Manterola, M L Pedro-Botet, A Raventos, M Foz
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  ABSTRACT: Tuberculous lymphadenitis (TL) is a very common infection in human immunodeficiency virus (HIV)-infected patients. We performed fine-needle aspiration biopsy (FNAB) of enlarged lymph nodes in 57 HIV-infected patients to evaluate its usefulness in this population. We observed three cytologic patterns in 21 patients diagnosed as having TL: granulomatous lymphadenitis (GL) in 4 FNABs, necrotizing granulomatous lymphadenitis (NGL) in 7 FNABs, and necrotizing lymphadenitis (NL) in 12 FNABs. GL and NGL are already well-known and considered to be highly suggestive of TL. Our results support the idea that NL should have the same diagnostic value as GL or NGL. In the group of 12 patients with NL, TL was confirmed in 11 by microbiologic methods (7 by a positive Ziehl-Neelsen stain and 4 by a positive Löwenstein culture) and in the remaining patient by a biopsy that showed NGL with acid-fast bacilli. We conclude that FNAB is a useful, inexpensive, and safe technique for diagnosing TL in HIV-infected patients. The finding of a NL pattern is suggestive enough of TL to start antituberculous treatment.
  Journal of acquired immune deficiency syndromes 01/1994; 6(12):1335-8.
• Article: Molecular epidemiology and Legionnaire's disease.

  M Sabrià-Leal, M L Pedro-Botet, L Ruiz, G Gimenez
  Infection Control and Hospital Epidemiology 12/1993; 14(11):616-7. · 3.67 Impact Factor
• Article: [Usefulness of the API ZYM system for the identification of Legionella sp].

  M L Pedro-Botet, M Sabria, M J Condom, L Espinosa, L Ruiz, J Arnal, M Foz
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  ABSTRACT: The identification of Legionella genus is usually difficult, time consuming, and expensive. We decided to study the usefulness of API-ZYM system (Biomerieux) for Legionella species identification. A total of 96 strains (82 from Legionella genus and 14 from other bacterial genus) were included. We studied 19 different enzyme activities and we proceed following the manufacturers directions, with an incubation time for galleries of 4 hours at 37 degrees C. Seventeen out of the 19 enzyme activities were uniformly positive or negative for Legionella sp. Thus, the sensitivity of these activities was 100% in identifying Legionella sp. Valine and cystine-arylamidase gave variable reactions for Legionella sp. strains. When the results of these enzyme test were compared to those of the control strains (Haemophilus influenzae, Pseudomonas aeruginosa) we observed that alkaline phosphatase, esterase, esterase-lipase, acid phosphatase and naphthol-AS-BI-phosphohydrolase in one side and esterase, esterase-lipase and leucin-arylamidase in the other side were able to differentiate Legionella sp. strains from P. aeruginosa and H. influenzae, respectively. API SYM system is a simple, accurate and reproducible method for the identification of Legionella sp.
  Enfermedades Infecciosas y Microbiología Clínica 04/1993; 11(3):131-4. · 1.49 Impact Factor