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ABSTRACT: Perfluorinated compounds (PFCs) were measured in fish samples and blood plasma of anglers in a cross-sectional study at Lake Möhne, Sauerland area, Germany. Human plasma and drinking water samples were analyzed by solid phase extraction, high-performance liquid chromatography (HPLC), and tandem mass spectrometry (MS/MS). PFCs in fish fillet were measured by ion pair extraction followed by HPLC and MS/MS. PFOS concentrations in 44 fish samples of Lake Möhne ranged between 4.5 and 150 ng/g. The highest median PFOS concentrations have been observed in perches (median: 96 ng/g) and eels (77 ng/g), followed by pikes (37 ng/g), whitefish (34 ng/g), and roaches (6.1 ng/g). In contrast, in a food surveillance program only 11% of fishes at retail sale contained PFOS at detectable concentrations. One hundred five anglers (99 men, 6 women; 14-88 years old; median 50.6 years) participated in the human biomonitoring study. PFOS concentrations in blood plasma ranged from 1.1 to 650 μg/L (PFOA: 2.1-170 μg/L; PFHxS: 0.4-17 μg/L; LOD: 0.1 μg/L). A distinct dose-dependent relationship between fish consumption and internal exposure to PFOS was observed. PFOS concentrations in blood plasma of anglers consuming fish 2-3 times per month were 7 times higher compared to those without any fish consumption from Lake Möhne. The study results strongly suggest that human internal exposure to PFC is distinctly increased by consumption of fish from PFC-contaminated sites.
Environmental Science & Technology 05/2011; 45(19):8046-52. · 4.80 Impact Factor
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Monika Kasper-Sonnenberg,
Dorothee Sugiri,
Sabine Wurzler,
Ulrich Ranft,
Heinrich Dickel,
Jürgen Wittsiepe,
Jürgen Hölzer,
Friederike Lemm,
Georg Eberwein,
Peter Altmeyer, Martin Kraft,
Ursula Krämer,
Michael Wilhelm
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ABSTRACT: In a cross-sectional study performed in 2000, an unexpected positive association between nickel (Ni) in ambient air, urinary Ni content and the prevalence of Ni sensitization in a subgroup of 6-yr-old children living near a steel mill was observed. Between 2005 and 2006, in a different and larger study population, we examined if Ni from ambient air or urinary Ni concentration was related to Ni sensitization in children living next to Ni-emitting steel mills. We studied 749 school beginners living in four Ni-polluted industrial areas of North Rhine-Westphalia, Germany. We assessed Ni in ambient air, Ni in urine from children and mothers, and Ni in tap water, conducted patch tests in children (including the NiSO(4)-dilution test) and collected questionnaire data. Statistics were done by linear and logistic regression analyses, adjusted for covariates. At increased Ni concentration in ambient air (unit of increase: 10 ng/m(3)), urinary Ni concentrations rose in both mothers (9.1%; 95% CI: 6.8-11.4%) and children (2.4%; 95% CI: 0.4-4.4%). The prevalence of Ni sensitization in children was associated with increased Ni from ambient air (unit of increase: 18 ng/m(3); odds ratio 1.28; 95% CI: 1.25-1.32) and urinary Ni concentration (unit of increase: 7.1 μg/L; odds ratio 2.4; 95% CI: 1.20-4.48). Ni in ambient air of areas with Ni-emitting factories contributes to internal Ni exposure in residents via inhalation and, furthermore, is a risk factor for the development of Ni sensitization in children.
Environmental Research 02/2011; 111(2):266-73. · 3.40 Impact Factor
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ABSTRACT: Residents in Arnsberg, Germany, had been supplied by drinking water contaminated with perfluorooctanoate (PFOA). Biomonitoring data from 2006 evidenced that plasma PFOA concentrations of residents from Arnsberg were 4.5-8.3 times higher than those in reference groups. The introduction of charcoal filtration in July 2006 distinctly reduced PFOA concentrations in drinking water. Our one-year follow-up study showed a 10-20% reduction of PFOA plasma levels in residents from Arnsberg. Here we report the first results of the two-year follow-up study Arnsberg 2008. Additionally, the results of the two-year follow-up examination of the reference group are included. Paired plasma samples of 138 study participants (45 children, 46 mothers and 47 men) collected in 2006 and 2008 were considered in the statistical analyses. Within the two years plasma concentrations of PFOA, perfluorooctanesulfonate (PFOS) and perfluorohexanesulfonate (PFHxS) decreased in residents from Arnsberg and in control groups. The geometric means of PFOA plasma levels declined by 39% (children and mothers) and 26% (men) in Arnsberg and by 13-15% in the corresponding subgroups from the reference areas. For the population from Arnsberg a geometric mean plasma PFOA half-life of 3.26 years (range 1.03-14.67 years) was calculated. Our results confirm an ongoing reduction of the PFOA load in residents from Arnsberg. The decline of PFC levels in plasma of participants from the reference areas reflects the general decrease of human PFC exposure during the very recent years.
International journal of hygiene and environmental health 06/2010; 213(3):217-23. · 2.64 Impact Factor
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ABSTRACT: In Arnsberg, Sauerland area Germany, 40000 residents were exposed to PFOA-contaminated drinking water (500-640ng PFOA/l; May 2006). In July 2006, the PFOA-concentrations in drinking water were lowered significantly by activated charcoal filtering in the waterworks, mostly below the limit of detection (10ng/l). A first human biomonitoring study performed in autumn 2006 revealed that PFOA-concentrations in blood plasma of residents living in Arnsberg were 4.5-8.3 times higher than in the reference groups. One year after the first survey, all participants (2006: 164 mothers, 90 children, 101 men) were invited to take part in a follow-up study. It was the aim of the study to determine the decline of the PFOA-concentrations in blood plasma. 288 persons (81%) were included in the statistical analysis. The (geometric) mean PFOA-concentrations in blood plasma of Arnsberg's residents decreased from 22.1 to 17.4 microg/l in children, from 23.4 to 18.8 microg/l in mothers and from 25.3 to 23.4 microg/l in men within one year. The average (geometric mean) changes in each individual's PFOA-concentrations were approximately 10 (men), 17 (mothers) and 20 (children) percent/year. The observed decline in PFOA-concentrations indicates a slow elimination in humans. This finding in groups of the general population is in agreement with data on long elimination half-lives observed in occupationally exposed workers.
International journal of hygiene and environmental health 06/2009; 212(5):499-504. · 2.64 Impact Factor
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ABSTRACT: Growing evidence indicates that environmental exposures are more prevalent among socially disadvantaged groups. We investigated the distribution of environmental exposures and health outcomes in preschool children, and examined the role of social position on their associations.
We analysed data from a cross-sectional study on 968 preschool children from three districts in the Ruhr Area and one rural community in North Rhine-Westphalia in 2000. Parents filled in a questionnaire on socio-demographic characteristics, environmental exposures, respiratory infections and allergic diseases. Residential annual total suspended particulate matter (TSP) mass concentrations were derived from a small-scale interpolation model. Lung function, allergic sensitisation and immunologic function were assessed. We analysed the associations between environmental exposures and health outcomes in social subgroups with logistic regression.
High TSP concentrations at the home address and unfavourable living conditions were more prevalent in the socially disadvantaged groups, while allergic and respiratory infectious diseases were reported more frequently in the privileged groups. The odds ratio for the association between TSP and history of allergic diseases was 1.17 (95% CI 0.95-1.45) in children without immigration background and 0.71 (95% CI 0.53-0.95) with immigration background. Heterogeneity for exposure-outcome associations was also seen between TSP and lung function as well as unfavourable living conditions and allergic diseases.
We found evidence for an influence of social position on environment-health associations. Possible explanations for heterogeneity include social group-specific over- and underreporting and effect measure modification, which need to be taken into account when designing and analysing environmental health studies.
International journal of hygiene and environmental health 06/2008; 212(2):146-56. · 2.64 Impact Factor
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ABSTRACT: 40,000 residents in Arnsberg, Germany, had been exposed to drinking water contaminated with perfluorinated compounds (PFCs). Internal exposure of the residents of Arnsberg to six PFCs was assessed in comparison with reference areas.
One hundred seventy children (5-6 years of age), 317 mothers (23-49 years), and 204 men (18-69 years) took part in the cross-sectional study.
Individual consumption of drinking water and personal characteristics were assessed by questionnaire and interview. Perfluorooctanoate (PFOA), perfluorooctanesulfonate (PFOS), perfluorohexanoate, perfluorohexanesulfonate (PFHxS), perfluoropentanoate, and perfluorobutanesulfonate (PFBS) in blood plasma and PFOA/PFOS in drinking water samples were measured by solid-phase extraction, high-performance liquid chromatrography, and tandem mass spectrometry detection.
Of the various PFCs, PFOA was the main compound found in drinking water (500-640 ng/L). PFOA levels in blood plasma of residents living in Arnsberg were 4.5-8.3 times higher than those for the reference population (arithmetic means Arnsberg/controls: children 24.6/5.2 microg/L, mothers 26.7/3.2 microg/L, men 28.5/6.4 microg/L). Consumption of tap water at home was a significant predictor of PFOA blood concentrations in Arnsberg. PFHxS concentrations were significantly increased in Arnsberg compared with controls (p < 0.05). PFBS was detected in 33% of the children, 4% of the women, and 13% of the men in Arnsberg compared with 5%, 0.7%, and 3%, respectively, in the reference areas (p < 0.05). Regression analysis showed that age and male sex were significant predictors of PFOS, PFOA, and PFHxS; associations of other regressors (diet, body mass index) varied among PFCs.
PFC concentrations in blood plasma of children and adults exposed to PFC-contaminated drinking water were increased 4- to 8-fold compared with controls.
Environmental Health Perspectives 06/2008; 116(5):651-7. · 7.04 Impact Factor
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Yuguang Cao,
Gerhard Winneke,
Michael Wilhelm,
Jürgen Wittsiepe,
Friederike Lemm,
Peter Fürst,
Ulrich Ranft,
Matthias Imöhl, Martin Kraft,
Barbara Oesch-Bartlomowicz,
Ursula Krämer
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ABSTRACT: Endocrine dysfunction related to the hypothalamic-pituitary-thyroid (HPT) and/or the hypothalamic-pituitary-gonadal axis (HPG) is being discussed as underlying developmental adversity of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) and polychlorinated biphenyls (PCBs). This study was done to evaluate effects related to the HPG axis.
A birth-cohort study was initiated in the year 2000. Healthy mother-infant pairs were recruited in the industrialized city of Duisburg, Germany. Dioxins, dioxin-like PCBs and six indicator PCBs were measured in maternal blood during pregnancy and in maternal milk. Testosterone and estradiol levels were measured in maternal and cord serum of 104 mother-infant pairs representing a subsample with a complete data set of the total basic sample of 232 participants. Linear regression analysis was used to describe the association of PCDD/Fs or PCB in maternal blood or milk with sex steroid concentrations after adjustment for confounding.
Median concentrations for PCDD/Fs in maternal blood fat and milk fat in terms of WHO-TEq were 15.3 and 13.1pg WHO-TEq/g, respectively, and for the sum of the indicator PCBs (#28, #52, #101, #138, #153, #180) 149 and 177ng/g. The adjusted ratio of geometric means when doubling the concentration of PCDD/Fs in maternal blood fat was 0.86, 95% confidence interval (95% CI): 0.72-1.03 for testosterone and 0.73 (0.61-0.87) for estradiol in cord serum. Typically, testosterone reduction was more pronounced in cord serum of female and estradiol reduction in that of male babies. Reduction of hormone levels was generally more pronounced for dioxins than for indicator PCBs.
The hypothalamic-pituitary-gonadal axis of newborn babies is influenced by prenatal exposure to PCDD/Fs and PCBs in a manner suggestive of AhR-mediation. The clinical relevance of this finding remains to be established, however.
International Journal of Hygiene and Environmental Health 04/2008; 211(1-2):30-9. · 3.81 Impact Factor
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ABSTRACT: In May 2006 the first serious German perfluorinated compounds (PFC) case of contamination became evident. Industrial waste with high concentrations of PFC was manufactured into a soil improver by a recycling company and spread by farmers on agricultural land of the rural area Sauerland, and led to substantial environmental pollution. In parts of the affected area, perfluorooctanoic acid (PFOA) concentrations in drinking water were > 0.5 microg/L. The German Drinking Water Commission assessed PFC in drinking water and set a health-based guidance value for safe lifelong exposure of all population groups at 0.3 microg/L (sum of perfluorooctane sulfonate [PFOS] and PFOA). The Ministry of Environment together with regional institutions initiated monitoring measurements and actions to minimize further contamination. A human biomonitoring study with mother-child pairs and men revealed that increased PFOA exposure via drinking water led to about four- to eightfold higher PFOA levels in plasma compared to nonexposed groups. Analysis of PFC in breast milk showed comparatively low levels, which seemed not to pose a risk for lactating infants. Due to high levels of PFOS in fish from contaminated lakes and rivers, recommendations for anglers to reduce fish consumption were initiated. Remediation of the affected area is ongoing and PFC levels in various matrices are still above background levels.
Journal of Toxicology and Environmental Health Part A 02/2008; 71(11-12):725-33. · 1.83 Impact Factor
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Michael Wilhelm,
Jürgen Wittsiepe,
Friederike Lemm,
Ulrich Ranft,
Ursula Krämer,
Peter Fürst,
Stefan-Christian Röseler,
Michaela Greshake,
Matthias Imöhl,
Georg Eberwein,
Knut Rauchfuss, Martin Kraft,
Gerhard Winneke
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ABSTRACT: Prenatal exposure to polychlorinated biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) can affect neurobehavioral development of infants and children. This effect may be mediated through disruption of thyroid hormone homeostasis. However, epidemiological studies reveal no consistent influence of PCDD/Fs and PCBs on thyroid status and neurodevelopment at environmental background levels. The effects may resolve with time of further decreasing exposure to these compounds. The aim of this study was to find out if there are still effects related to prenatal PCDD/F and PCB observable at the meanwhile decreased levels of exposure by using the same methods which have been applied in similar studies during the last 10 years in Europe. The birth cohort study was initiated in the year 2000 in the industrialized city of Duisburg, Germany. 232 healthy mother-infant pairs were recruited between 2000 and 2002. Dioxins, dioxin-like PCBs and six indicator PCBs were analyzed in maternal blood during pregnancy and in maternal milk following extraction and sample clean-up by HRGC/HRMS. Thyroid stimulating hormone (TSH), total thyroxine (T4), total triiodothyronine (T3), free thyroxine (FT4) and free triiodothyronine (FT3) were measured in serum samples of the pregnant women and in cord serum samples by chemiluminescent immunometric assay. Neurological examinations were performed at ages 2 weeks and 18 months using the neurological optimality score (NOS), mental and motor development were assessed using the Bayley Scales of Infant Development (BSID) at ages 12 and 24 months. Multiple linear regression analysis was used to describe the association of PCDD/F and PCB in maternal blood or milk with the outcome measurements after adjustment for confounding. Blood levels (n=182) of WHO 2005 toxic equivalents (TEQ) (PCDD/F+PCB) were in the range of 3.8-58.4 pg/glipid base (median: 19.3 pg/glipid base). The corresponding data for human milk (n=149) were 2.6-52.4 pg/glipid base (median: 19.7 pg/glipid base). Multiple regression analysis showed no decrease of thyroid hormones related to WHO 2005 TEQ in blood and milk of mothers and their newborns. Furthermore, no associations between exposure and neurological and developmental measures were observed. This study supports the view that the current decreased exposure to PCDD/Fs and PCBs does not impair thyroid function of newborns and neurodevelopment of infants until the age of 24 months.
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 12/2007; 659(1-2):83-92. · 2.85 Impact Factor
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ABSTRACT: Blood samples of pregnant women aged between 19 and 42 years at the time they gave birth and milk samples from the same women following delivery were collected between September 2000 and January 2003 from 169 participants living in an industrialized area of Germany (Duisburg birth cohort study). All samples were analyzed for their content of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/F) as well as dioxin-like and indicator polychlorinated biphenyls (PCB). Levels of WHO-TEq were in the range of 4.34-97.3 pg/g(lipid base) (median: 26.37, arithmetic mean: 28.36) for blood, or 3.01-78.7 pg/g(lipid base) (median: 26.40, arithmetic mean: 27.27) for milk, respectively. The four congeners 12378-PeCDD, 23478-PeCDF, 33'44'5-PeCB (# 126) and 233'44'5-HxCB (# 156) contribute the main share to total WHO-TEq. The contribution of PCDD/F in relation to PCB to total WHO-TEq was 60:40% in blood and 52:48% in milk. Good correlations of the contaminant levels in lipid base between both matrices were found. The distribution between blood and milk depends on the molecular weight of the substances. Higher chlorinated PCDD/F- and PCB-congeners were found in 2-4-fold higher concentrations in blood in relation to milk and the concentrations of lower chlorinated PCB-congeners were up to 2-fold higher in milk in relation to blood. The body burden of PCDD/F and PCB increases with age and decreases over the total nursing period. Women who had lived outside highly industrialized countries showed lower concentrations of PCDD/F and PCB. In some cases, elevated levels of PCB were observed when the women had previously lived in Eastern Europe for a long time. In comparison with recent data, the decline in human PCDD/F and PCB levels observed during the nineties seems to have stopped. The individual exposures of the infants due to breastfeeding within the first 18 months were calculated to be from 4.4 to 318 ng WHO-TEq (median: 106, arithmetic mean: 118). The actual mean daily exposure of a breastfed infant can be estimated to 131 pg WHO-TEq/kg(body weight).
Chemosphere 05/2007; 67(9):S286-94. · 3.21 Impact Factor
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ABSTRACT: Living close to major roads or highways has been suggested to almost double the risk of dying from cardiopulmonary causes. We assessed whether long-term exposure to air pollution originating from motorized traffic and industrial sources is associated with total and cause-specific mortality in a cohort of women living in North Rhine-Westphalia, Germany.
The study was a follow-up of a series of cross-sectional studies carried out during the 1980s and 1990s on the health of women (age 50-59 years). Approximately 4800 women were followed up for vital status and migration. Exposure to air pollution was defined by distance to major roads calculated from Geographic Information System data and by 1- and 5-year average nitrogen dioxide (NO2) and particle (PM10) concentrations calculated from air monitoring station data. We analyzed associations between exposure and mortality using Cox's proportional hazards models adjusting for confounders. Relative risks (RRs) refer to an interquartile range increase in exposure (16 microg/m for NO2; 7 microg/m for PM10).
During the follow-up period, 8% of the women died, 3% from cardiopulmonary causes. Cardiopulmonary mortality was associated with living within a 50-meter radius of a major road (adjusted RR = 1.70; 95% confidence interval = 1.02-2.81), with NO2 (1.57; 1.23-2.00 for 1-year average), and with PM10 (1.34; 1.06-1.71 for 1-year average). Exposure to NO2 was also associated with all-cause mortality (1.17; 1.02-1.34). No association was seen with noncardiopulmonary nonlung cancer mortality.
Living close to major roads and chronic exposure to NO2 and PM10 may be associated with an increased mortality due to cardiopulmonary causes.
Epidemiology 10/2006; 17(5):545-51. · 5.57 Impact Factor
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ABSTRACT: The presented overview concerning health relevant effects caused by nitrogen dioxide (NO2) resumes the current state of results from animal experiments and human studies (epidemiology and short-term chambers studies). NO2 concentrations applied in animal experiments were mostly considerably higher than in ambient air. Therefore, short- and long-term limit values were derived from human data. Experimental studies conducted with humans demonstrate effects after short-term exposure to concentrations at or above 400 microg NO2/m3. Effects on patients with light asthma could not be observed after short-term exposure to concentrations below 200 microg/m3. On basis of epidemiological long-term studies a threshold below which no effect on human health is expected could not be specified. Two short-term limit values have been proposed to protect public health: a 1-h value of 100 microg/m3 and a 24-h mean value of 50 microg/m3. Due to the limitations of epidemiological studies to disentangle effects of single pollutants, a long-term limit value cannot be easily derived. However, applying the precautionary principle, it is desirable to adopt an annual mean of 20 microg NO2/m3 as a long-term mean standard to protect public health.
International Journal of Hygiene and Environmental Health 02/2005; 208(4):305-18. · 3.81 Impact Factor
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ABSTRACT: In May 2006, a serious environmental contamination with perfluorinated compounds (PFCs) became evident in a rural area of North Rhine-Westphalia (NRW) (Region Sauerland), Germany. In autumn 2006, we performed a human biomonitoring study in which a 4–8-fold increase in perfluorooctanoate (PFOA)-plasma concentrations of children, their mothers and men living in Arnsberg (District Hochsauerlandkreis, NRW) was observed compared with a reference population. The exposure was clearly related to the consumption of PFOA-contaminated tap water. However, there is no clear information on the duration of this contamination. The current investigation involves the analysis of PFCs in 30 blood samples of young adults (age 20–31 years) who had ever lived in the affected area. The samples were taken between 1977 and 2004 and stored at the German Environmental Specimen Bank for Human Tissues. Analyses of PFOA, perfluoroctanesulfonate (PFOS), perfluorohexanoate (PFHxA), perfluorohexanesulfonate (PFHxS), perfluoropentanoate (PFPA) and perfluorobutanesulfonate (PFBS) in blood plasma were performed by solid-phase extraction, HPLC and MS/MS detection. PFOA values (median, range) were 6.1, 1.7–40.7 μg/l, PFOS values were 18.8, 8.1–150.7 μg/l and PFHxS values were 1.7, 0.5–4.6 μg/l. The concentrations of PFHxA, PFPA and PFBS in plasma were all below limit of detection. Time-trend analysis showed that between 1977 and 2004 PFOA and PFOS levels remained fairly stable. PFOS and PFOA levels were in the range of current background levels of the general population. In contrast, PFHxS plasma levels have steadily increased since 1977. There was a close association between PFOS and PFOA-plasma levels. From this pilot study there are no indications for an increased exposure to PFCs of residents in Arnsberg in the years 1977–2004 prior to the contamination in 2006.
International Journal of Hygiene and Environmental Health.
