Yasir Hasan Siddique

Aligarh Muslim University, Koil, Uttar Pradesh, India

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Publications (53)61.12 Total impact

  • Yasir Hasan Siddique, Smita Jyoti, Falaq Naz
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    ABSTRACT: ABSTRACT Parkinson's disease (PD) is characterized by progressive loss of dopaminergic neurons and aggregation of alpha synuclein (αS) in the brain. The role of epicatechin gallate (EG) was studied on the transgenic Drosophila model of flies expressing normal human alpha synuclein (h-αS) in the neurons. The objectives of the present work include the study of the effect of EG on the climbing ability, lipid peroxidation, and apoptosis in the brain of PD model flies. These flies exhibit locomotor dysfunction as the age progresses. EG at final concentration of 0.25, 0.50, and 1.0 μg/mL was supplemented in diet and flies were allowed to feed for 24 days. The climbing ability was assessed after 24 days. The supplementation of 0.25, 0.50, and 1.0 μg/mL of EG showed a dose-dependent significant delay in the loss of climbing ability and reduced the oxidative stress and apoptosis in the brain of PD model flies.
    Journal of Dietary Supplements 06/2014; 11(2):121-30.
  • Fahad Ali, Rahul, Falaq Naz, Smita Jyoti, Yasir Hasan Siddique
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    ABSTRACT: A number of pharmacological properties have been attributed to apigenin. In the present study the effect of apigenin was investigated with respect to hepatotoxicity induced by N-nitrosodiethylamine (NDEA), a compound that is present in many food stuffs and has been reported to be a hepatocarcinogen. Male rats were exposed to NDEA (0.1mg/ml) dissolved in drinking-water separately, and with 10, 20, or 40mg/ml of apigenin for 21 days. The activity of glutamic-oxaloacetic transaminase (SGOT), glutamic-pyruvic transaminase (SGPT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) was measured in blood serum. Lipid peroxidation, protein carbonyl content and micronucleus frequency were determined in hepatocytes. To assess the effect on DNA damage, the comet assay was performed on hepatocytes, blood lymphocytes and bone-marrow cells of the exposed rats. The results of the study reveal that the treatment of NDEA together with apigenin showed a significant dose-dependent decrease in the serum concentration of the enzymes SGOT, SGPT, ALP and LDH (p<0.05). Histological sections of the liver also showed a protective effect of apigenin. A significant dose-dependent reduction in lipid peroxidation and protein carbonyl content was observed in rats exposed to NDEA (0.1mg/ml) together with apigenin (p<0.05). The results obtained for the comet assay in rat hepatocytes, blood lymphocytes and bone-marrow cells showed a significant dose-dependent decrease in the mean tail length (p<0.05). The present study supports the role of apigenin as an anti-genotoxic and hepatoprotective agent.
    Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 04/2014; · 3.90 Impact Factor
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    Yasir Hasan Siddique, Falaq Naz, Smita Jyoti
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    ABSTRACT: Background. A time dependent loss of dopaminergic neurons and the formation of intracellular aggregates of alpha synuclein have been reported in PD model flies. Methods. The progeny (PD flies) expressing human alpha synuclein was exposed to 25, 50, and 100 µM of curcumin mixed in the diet for 24 days. The effect of curcumin was studied on lifespan, activity pattern, oxidative stress, and apoptosis in the brains of PD model flies. The activity of PD model flies was monitored by using Drosophila activity monitors (DAMs). For the estimation of oxidative stress, lipid peroxidation and protein carbonyl content were estimated in the flies brains of each treated groups. The cell death in Drosophila brain was analyzed by isolating brains in Ringer's solution placing them in 70% ethanol and stained in acridine orange to calculate the gray scale values. Results. The exposure of flies to 25, 50, and 100 µM of curcumin showed a dose dependent significant delay in the loss of activity pattern, reduction in the oxidative stress and apoptosis, and increase in the life span of PD model flies. Conclusion. Curcumin is potent in reducing PD symptoms.
    BioMed Research International 01/2014; 2014:606928. · 2.88 Impact Factor
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    ABSTRACT: Introduction The effect of Bacopa monnieri leaf extract was studied on transgenic Drosophila (Parkinson's disease) model flies which expressed normal human alpha synuclein (h-αS) in their neurons. A time dependent loss of dopaminergic neurons and the formation of intracellular aggregates of αS (Lewy bodies) has been reported in the PD model flies. Methods B. monnieri leaf extract, prepared in acetone was subjected to GC-MS analysis. Diets with final concentrations of 0.25, 0.50 and 1.0 μl/ml were established and the flies were allowed to feed on for 24 days. The effect of the extracts was studied on the climbing ability, activity pattern, oxidative stress (lipid peroxidation and protein carbonyl content) and apoptosis in the brain of PD model flies. Results The exposure of PD flies to 0.25, 0.5 and 1.0 μl/ml showed a dose dependent significant delay in the loss of climbing ability as well as activity pattern, reduced the oxidative stress and apoptosis compared to untreated PD flies. Conclusion B. monnieri leaf extract improves behavioral abnormalities, reduces the oxidative stress and apoptosis in the brains of PD model flies.
    European Journal of Integrative Medicine 01/2014; · 0.56 Impact Factor
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    ABSTRACT: In the present study the graphene zinc oxide nanocomposite (GZNC) was synthesized, characterized, and evaluated for its toxic potential on third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg (9) . The synthesized GZNC was characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The GZNC in 0.1% dimethyl sulphoxide (DMSO) was sonicated for 10 minutes and the final concentrations 0.033, 0.099, 0.199, and 3.996 μg/μL of diet were established. The third instar larvae were allowed to feed on it separately for 24 and 48 hr. The hsp70 expression was measured by o-nitrophenyl-β-D-galactopyranoside assay, tissue damage was measured by trypan blue exclusion test, and β-galactosidase activity was monitored by in situ histochemical β-galactosidase staining. Oxidative stress was monitored by performing lipid peroxidation assay and total protein estimation. Ethidium bromide/acridine orange staining was performed on midgut cells for apoptotic index and the comet assay was performed for the DNA damage. The results of the present study showed that the exposure of 0.199 and 3.996 μg/μL of GZNC was toxic for both 24 hr and 48 hr of exposure. The doses of 0.033 μg/μL and 0.099 of GZNC showed no toxic effects on its exposure to the third instar larvae for 24 hr as well as 48 hr of duration.
    BioMed Research International 01/2014; 2014:382124. · 2.88 Impact Factor
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    Fahad Ali, Rahul, Falaq Naz, Smita Jyoti, Yasir Hasan Siddique
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    ABSTRACT: A number of pharmacological properties have been attributed to apigenin. In the present study the effect of apigenin was investigated with respect to hepatotoxicity induced by N-nitrosodiethylamine (NDEA), a compound that is present in many food stuffs and has been reported to be a hepatocarcinogen. Male rats were exposed to NDEA (0.1 mg/ml) dissolved in drinking-water separately, and with 10, 20, or 40 mg/ml of apigenin for 21 days. The activity of glutamic-oxaloacetic transaminase (SGOT), glutamic-pyruvic transaminase (SGPT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) was measured in blood serum. Lipid peroxidation, protein carbonyl content and micronucleus frequency were determined in hepatocytes. To assess the effect on DNA damage, the comet assay was performed on hepatocytes, blood lymphocytes and bone-marrow cells of the exposed rats. The results of the study reveal that the treatment of NDEA together with apigenin showed a significant dose-dependent decrease in the serum concentration of the enzymes SGOT, SGPT, ALP and LDH (p < 0.05). Histological sections of the liver also showed a protective effect of apigenin. A significant dose-dependent reduction in lipid peroxidation and protein carbonyl content was observed in rats exposed to NDEA (0.1 mg/ml) together with apigenin (p < 0.05). The results obtained for the comet assay in rat hepatocytes, blood lymphocytes and bone-marrow cells showed a significant dose-dependent decrease in the mean tail length (p < 0.05). The present study supports the role of apigenin as an anti-genotoxic and hepatoprotective agent.
    Mutation Research/Genetic Toxicology and Environmental Mutagenesis 01/2014; · 2.22 Impact Factor
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    Smita Jyoti, Saif Khan, Mohammad Afzal, Falaq Naz, Yasir Hasan Siddique
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    ABSTRACT: Oral submucosus fibrosis (OSMF) is a collagen-related disorder seen in habitual betel quids and smokers. This is a high risk precancerous condition in which the connective tissue fibers of the lamina propria and deeper parts of the mucosa becomes stiff with restricted mouth opening. Patients with severe cases have symptoms like difficulties in chewing, swallowing and speaking. In the present study 25 individuals were gutkha chewers and 25 were OSMF patients (chewing gutkha along with smoking) and 25 individuals were taken as controls. A significant increase in the frequency of micronuclei was observed in OSMF patients (34.4 ± 1.79) as compared to gutkha chewers (14.4 ± 0.73) and controls (4.36 ± 0.27). The number of micronucleated cells in OSMF, gutkha chewers and control groups were 19.84 ± 0.69, 12.6 ± 0.51 and 4.20 ± 0.27, respectively and are significantly different at p < 0.05. Acridine orange is used due its fluorescence nature and easier visibility of the micronucleus present in the buccal epithelial cells. It is concluded that chewing gutkha along with smoking is more dangerous for human health as it hastens the incidence of OSMF.
    Egyptian Journal of Medical Human Genetics 04/2013; 14(2):189–193.
  • Yasir Hasan Siddique, Syed Faiz Mujtaba, Smita Jyoti, Falaq Naz
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    ABSTRACT: The role of Eucalyptus citriodora L. leaf extract was studied on the transgenic Drosophila model of flies expressing normal human alpha synuclein (h-αS) in the neurons. These flies exhibit locomotor dysfunction as the age progresses. The leaf extract was prepared in acetone and was subjected to GC-MS analysis. The GC-MS analysis revealed the presence of 9 major compounds. E. citriodora extract at final concentration of 0.25, 0.50 and 1.0μl/ml was supplemented with the diet and the flies were allowed to feed for 21 days. The effect of extract was studied on the climbing ability and the oxidative stress on the PD model Drosophila expressing normal human alpha synuclein (h-αS) in the neurons. The supplementation of 0.25, 0.50 and 1.0μl/ml of E. citriodora extract showed a dose dependent significant delay in the loss of climbing ability and reduction in the oxidative stress in the brain of PD model flies. The results also support the utility of this model in studying PD symptoms.
    Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 01/2013; · 2.99 Impact Factor
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    Smita Jyoti, Saif Khan, Falaq Naz, Rahul, Fahad Ali, Yasir Hasan Siddique
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    ABSTRACT: In the present study the comet assay was performed in buccal epithelial cells to evaluate DNA damage among pan masala or gutkha chewers and smokers. The assay is a rapid, suitable and sensitive method for detecting various forms of DNA damage at individual cell level. The study comprises 300 individuals of which 50 individuals were gutkha chewers along with smoking, 50 indi-viduals were pan masala chewers along with smoking, 50 individuals were gutkha chewers, 50 indi-viduals were pan masala chewers, 50 individuals were smokers and 50 individuals were non-users (control) or not having any addiction. Comet tail length was observed to measure the extent of DNA damage. In all groups a significant increase in the tail length was observed as compared to the non-users (control). The highest tail length was observed among gutkha chewers along with smoking (36.9 ± 3.60). The results of the present study suggest that the panmasala and gutkha are genotoxic agents and induce DNA damage. Ó 2013 Production and hosting by Elsevier B.V. on behalf of Ain Shams University.
    01/2013;
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    ABSTRACT: Graphene, a two-dimensional carbon sheet with single-atom thickness, have attracted the scientific world for its potential applications in various field including the biomedical areas. In the present study the graphene copper nanocomposite (GCNC) was synthesized, characterized and evaluated for its toxic potential on third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg(9) . The synthesized GCNC was analyzed by X-ray diffraction (XRD), scanning/transmission electron microscopy (SEM/TEM), atomic force microscopy (AFM), and fourier transform infrared spectroscopy (FTIR). The GCNC in 0.1% DMSO was sonicated for 10 min and the final concentration of 0.033, 0.099, 0.199 and 3.996 µg/µl of diet were established. The third instar larvae were allowed to feed on it separately for 24 and 48 hrs. The hsp70 expression was measured by O-nitrophenyl-β-D-galactopyranoside assay, tissue damage by trypan blue exclusion test and β-galactosidase activity was monitored by in situ histochemical β-galactosidase staining. Oxidative stress was monitored by performing lipid peroxidation assay and total protein estimation. Ethidium bromide/acridine orange staining was performed on midgut cells for apoptotic index and the comet assay was performed for the DNA damage. The results of the present study showed that the exposure of 0.199 and 3.996 µg/µl of GCNC were toxic for 24 hr of exposure and for 48 hr of exposure: 0.099, 0.199 and 3.996 µg/µl of GCNC was toxic. The dose of 0.033 µg/µl of GCNC showed no toxic effects on its exposure to the third instar larvae for 24 hr as well as 48 hrs. This dose can be considered as No Observed Adverse Effect Level (NOAEL).
    PLoS ONE 01/2013; 8(12):e80944. · 3.53 Impact Factor
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    ABSTRACT: Synthetic progestins and estrogens have been reported to be toxic in various experimental models. Their prolonged use has been reported to induce cancer in humans. In the present study the effects of oral contraceptives were studied among users using chromosomal aberrations, sister chromatid exchanges and DNA damage as a parameter, in cultured human peripheral blood lymphocytes. The study was performed on 25 women (users) and 25 age match controls. No significant difference was observed in chromosomal aberrations and DNA damage. A significant increase was observed in sister chromatid exchanges (SCEs) Cell among users. The results obtained and the risk of oral contraceptives (OCs) genotoxicity have been discussed.
    Egyptian Journal of Medical Human Genetics 10/2012; 13(3):301–305.
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    ABSTRACT: All living organisms respond to various physical or chemical stressors by the induction of heat shock protein (HSP). The present study was performed on transgenic Drosophila melanogaster (hsp70-lacZ) Bg(9) in which the transformation vector is inserted with a P-element, the line contains wild-type hsp70 sequence up to the lacZ fusion point. The effect of L-ascorbic acid on the hsp70 expression and tissue damage was studied at the doses of 1, 2, 4, and 8 × 10(-4) g/ml in the third instar larvae of transgenic D. melanogaster (hsp70-lacZ) Bg(9). The larvae were exposed to different doses of L-ascorbic acid for 24 and 48 hours. A dose-dependent significant increase in the hsp70 expression was observed at 2, 4, and 8 × 10(-4) g/ml of L-ascorbic acid for both 24 and 48 hours. The tissue damage was observed only in the 48 hours of exposure and mostly only in the salivary glands of the third instar larvae of transgenic D. melanogaster (hsp70-lacZ) Bg(9). The present study also validates and supports the use of transgenic D. melanogaster (hsp70-lacZ) Bg(9) for the toxicological evaluations.
    Toxicology International 09/2012; 19(3):301-5.
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    ABSTRACT: ABSTRACT In the present study, the effect of l-ascorbic acid (AA) was studied on the climbing ability of the Parkinson's disease (PD) model Drosophila expressing normal human alpha synuclein (h-αs) in the neurons. These flies show locomotor dysfunction as the age progresses. AA at final concentration of 11.35 × 10(-5) M, 22.71 × 10(-5) M, 45.42 × 10(-5) M, and 68.13 × 10(-5) M was added to the diet, and the flies were allowed to feed for 21 days. AA at 11.35 × 10(-5) M did not show any significant delay in the loss of climbing ability of PD model flies. However, AA at 22.71 × 10(-5) M, 45.42 × 10(-5) M, and 68.13 × 10(-5) M showed a dose dependent significant (p < .05) delay in the loss of climbing ability of PD model flies as compared to the untreated PD flies. The total protein concentration in brain homogenate was measured in treated as well as control groups after 21 days, no significant difference was obtained between treated as well as control (PD flies and l-dopa) groups. The results suggest that AA is potent in delaying the climbing disability of the PD model flies expressing h-αs in the neurons.
    The International journal of neuroscience 07/2012; · 0.86 Impact Factor
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    Yasir Hasan Siddique, Smita Jyoti, Falaq Naz, Mohammad Afzal
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    ABSTRACT: A method using 1-methyl-2-phenylindole was developed for the estimation of lipid peroxidation in third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg (9). The method is specific for the estimation of malonaldehyde. The larvae were exposed to 0.0025, 0.025, 0.050, and 0.100 μl/ml of cyclophosphamide for 24 and 48 h. The homogenate was prepared of the larvae tissue explant and the absorbance was noted at 586 nm. A significant dose-dependent increase in the mean absorbance values was observed for both 24 and 48 h of exposure as compared to the untreated group. On the basis of results obtained, it is suggested that the present method is more precise, accurate, and robust for the estimation of lipid peroxidation in the third instar larvae of transgenic D. melanogaster (hsp70-lacZ)Bg (9).
    Pharmaceutical methods. 07/2012; 3(2):94-7.
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    Yasir Hasan Siddique, Mohammad Afzal
    Journal of pharmacology & pharmacotherapeutics. 04/2012; 3(2):188-90.
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    Yasir Hasan Siddique, Gulshan Ara, Smita Jyoti, Mohammad Afzal
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    ABSTRACT: Parkinson's disease (PD) is characterized by the progressive loss of dopaminergic neurons and the aggression of alpha Synuclein (αS) in the brain. Drosophila mutants and transgenes have provided a platform to understand the mechanistic insight associated with the degenerative diseases. A number of polyphenols have been reported to inhibit the αS aggregation resulting in the possible prevention of PD. The involvement of free radicals in mediating the neuronal death in PD has also been implicated. In the present study, the effect of Nordihydroguaiaretic acid (NDGA) was studied on the climbing ability of the PD model Drosophila expressing normal human alpha synuclein (h-αS) in the neurons. These flies exhibit locomotor dysfunction as the age progresses. NDGA at final concentration of 0.01, 0.1, 0.5, and 1μl/ml was supplemented with the diet and the flies were allowed to feed for the 24 days. NDGA at 0.01 μl/ml did not showed any significant delay in the loss of climbing ability of PD model flies. However, NDGA doses at 0.1, 0.5, and 1.0 μl/ml showed a dose dependent significant (p < .05) delay in the loss of climbing ability of PD model flies as compared to the untreated PD flies. The results suggest that the NDGA is potent in delaying the climbing disability of PD model flies and also supports the utility of this model in studying PD symptoms.
    Journal of Dietary Supplements 03/2012; 9(1):1-8.
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    Smita Jyoti, Saif Khan, Mohammad Afzal, Yasir Hasan Siddique
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    ABSTRACT: Gutkha is a cheap and convenient betel quid substitute, which is popular among all age groups. Various studies reveal its carcinogenic nature that leads to oral submucosus fibrosis and increases the chances of oral cancer. The micronucleus (MN) assay in exfoliated mucosal cells is a useful method for observing genetic damage in humans. To observe the genotoxic effect of gutkha on human buccal epithelial cells. The MN assay was performed to assess the frequency of MN in human buccal epithelial cells. The study comprises 60 individuals of which 30 individuals were gutkha chewers and another 30 were nonusers (control). The MN frequency was scored to estimate the genotoxic damage. In gutkha users, the frequency of MN was highly significant (17.4 ± 0.944) as compared with nonusers (control) groups (4.53 ± 0.331) (P < 0.001). The MN assay in human buccal epithelial cells is a useful and minimally invasive method for monitoring genetic damage in humans. Asignificantly higher frequency of micronucleated cells are found among gutkha users.
    Advanced biomedical research. 01/2012; 1:35.
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    Yasir Hasan Siddique, Gulshan Ara, Mohammad Afzal
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    ABSTRACT: Malondialdehyde (MDA) is used for the estimation of damage by reactive oxygen species. MDA is a major reactive aldehyde resulting from the peroxidation of biological membranes. The most common method used to assess MDA production is the thiobarbituric acid (TBARS) assay. However, the value of this method is curbed by low specificity and has been criticized for its use in human studies. In the present study we have used an alternative method for the estimation of MDA production i.e. reaction of MDA with a chromogenic agent 1-methyl-2-phenylindole at 45°C. The paper describes the method of preparing standards for the estimation of MDA (lipid peroxidation) after the treatment with an oxidative stress inducing agent hydrogen peroxide (H(2)O(2)). In the present study, the treatments of 1, 5, 10, 20, 50, 100, 150 and 200 μM of H(2)O(2) induced significant increase in lipid peroxidation as compared to the untreated ones. The results suggest that the present method can be used to measure the lipid peroxidation in cultured human peripheral blood lymphocytes and is specific for MDA estimation.
    Dose-Response 01/2012; 10(1):1-10. · 1.50 Impact Factor
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    Yasir Hasan Siddique, Gulshan Ara, Mohammad Afzal
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    ABSTRACT: Abstract In the present study the effect of 0.1, 0.2, 0.4, 0.8, and 1.0 µL/mL of the steroid K-canrenoate was evaluated in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg(9) for 6, 24, and 48 hours of duration. The treatment of 0.1, 0.2, and 0.4 µL/mL of K-canrenoate did not induce the activity of hsp70 significantly compared to the control. The treatments of 0.8 and 1.0 µL/mL of K-canrenoate not only caused tissue damage but also induced a significant increase in the expression of hsp70 for the different durations of exposure. The results of the present study suggest that the K-canrenoate at 0.8 and 1.0 µL/mL is cytotoxic and caused tissue damage in the third instar larvae of transgenic D. melanogaster (hsp70-lacZ) Bg(9).
    Journal of Insect Science 01/2012; 12:92. · 0.88 Impact Factor
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    Vineet Kumar, Gulshan Ara, Mohammad Afzal, Yasir Hasan Siddique
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    ABSTRACT: Methyl methanesulfonate (MMS) is an anti-carcinogenic drug and its toxicity has been reported in various experimental models. The hsp70s are a family of ubiquitously expressed heat shock proteins. In the recent years, hsp70 has been considered to be one of the candidate genes for predicting cytotoxicity against environmental chemicals. Nowadays emphasis is given to the use of alternatives to mammals in testing, research and education. The European Centre for the Validation of Alternative Methods (EVCAM) has recommended the use of Drosophila as an alternative model for scientific studies. Almost all living organisms possess proteins with a similar structure to that of hsp70s. In the present study, the toxicity of MMS was evaluated by quantifying hsp70 expression and tissue damage in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg(9), at different doses and hours of exposure. We studied the effect of 0.25, 0.50, 0.75 and 1.0 µl/ml of MMS at 2, 4, 24 and 48 hours of exposure on hsp70 expression by using the soluble O-nitrophenyl-β-D-galactopyranoside (ONPG) assay and on establishing the tissue damage by the Trypan blue exclusion assay in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg(9). A dose-dependent increase in the expression of hsp70 was observed at 0.25, 0.50, and 0.75 µl/ml of MMS compared to the control. At the highest dose, i.e. 1.0 µl/ml of MMS, the activity of hsp70 was decreased due to tissue damage.
    Interdisciplinary toxicology 09/2011; 4(3):159-65.