Publications (28)22.7 Total impact
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Article: Effect of fullerenes C60 on X-protein amyloids
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ABSTRACT: The inhibitory effect of hydrated fullerene C60 and the sodium salt of the fullerene polycarboxylic derivative C60Cl(C6H4CH2COONa)5 on the formation of amyloid fibrils by X-protein in vitro has been studied by electron microscopy. It is shown that these compounds not only destroy mature amyloid fibrils but also prevent the formation of new fibrils. This property of fullerenes, which are nanoparticles, can be used to develop a novel medical nanotechnology in the therapy for amyloidoses.Biophysics 04/2012; 54(2):135-138. -
Article: Effect of nitroderivatives of fullerene C60 on amyloid fibrils of the brain Aβ(1–42) peptide and muscle X-protein
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ABSTRACT: The antiamyloidogenic capacity of water-soluble nitroderivatives of fullerene C60: methyl ester of L-N-[(2-nitroglyceryl) fullerenyl] proline, methyl ester of L-N-[(2,3-dinitroglyceryl) fullerenyl] proline, and 2-nitroxyethyl ester of L-N-([2-(nitroxy) ethyl] fullerenyl) proline has been studied in vitro by high-resolution electron microscopy. It was shown that these fullerene C60 nitroderivatives are able to prevent the formation of amyloid fibrils by the brain Aβ(1–42)-peptide and muscle X-protein and to destroy mature fibrils. Electron microscopy is a promising method for selecting effective antiamyloidogenic drugs. The antiamyloidogenic activity of nanodimensional fullerene C60 nitroderivatives offers strong possibilities for creating a new nanotechnology for the therapy of amyloidoses. Key wordsamyloids-muscle X-protein-Aβ peptides-fullerenes-amyloidoses-Alzheimer’s diseaseBiophysics 04/2012; 55(3):353-357. -
Article: Changes in isoform composition, structure, and functional properties of titin from Mongolian gerbil (Meriones unguiculatus) cardiac muscle after space flight.
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ABSTRACT: Changes in isoform composition, secondary structure, and titin phosphorylation in Mongolian gerbil (Meriones unguiculatus) cardiac muscle were studied after 12-day-long space flight onboard the Russian spacecraft Foton-M3. The effect of titin on the actin-activated myosin ATPase activity at pCa 7.5 and 4.6 was also studied. Almost twofold increase in titin long N2BA isoform content relative to that of short N2B isoform was found on electrophoregrams of cardiac muscle left ventricle of the flight group gerbils. Differences in secondary structure of titin isolated from cardiac muscle of control and flight groups of gerbils were found. An increase in phosphorylation (1.30-1.35-fold) of titin of cardiac muscle of the flight group gerbils was found. A decrease in activating effect of titin of cardiac muscle of the flight group gerbils on actomyosin ATPase activity in vitro was also found. The observed changes are discussed in the context of M. unguiculatus cardiac muscle adaptation to conditions of weightlessness.Biochemistry (Moscow) 12/2011; 76(12):1312-20. · 1.06 Impact Factor -
Article: Fullerenolates: metallated polyhydroxylated fullerenes with potent anti-amyloid activity.
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ABSTRACT: It has been revealed for the first time that sodium fullerenolate Na(4)[C(60)(OH)(∼30)] (NaFL), a water soluble polyhydroxylated [60]fullerene derivative, destroys amyloid fibrils of the Aβ(1-42) peptide in the brain and prevents their formation in in vitro experiments. The cytotoxicity of NaFL was found to be negligibly low with respect to nine different culture cell lines. At the same time, NaFL showed a very low acute toxicity in vivo. The maximal tolerable dose (MTD) and LD50 for NaFL correspond to 1000 mg kg(-1) and 1800 mg kg(-1), respectively, as revealed by in vivo tests in mice using intraperitoneal drug injection. The observed pronounced anti-amyloid activity and low toxicity of NaFL make it a very promising lead drug for the development of potent fullerene-based therapeutic approaches for the treatment of amyloidoses, such as Alzheimer's disease and others.Organic & Biomolecular Chemistry 06/2011; 9(16):5714-9. · 3.70 Impact Factor -
Article: Effects of Hydrated Forms of C60 Fullerene on Amyloid β-Peptide Fibrillization In Vitro and Performance of the Cognitive Task
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ABSTRACT: It has been shown for the first time by transmission electron microscopy that the hydrated fullerene C60 inhibited the fibrillization of amyloid-β25-35 peptide. The fullerene affected the amyloid-β25-35 assembly, manifesting its anti-amyloidogenic capacity. Our in vivo investigations demonstrated also that a single intracerebroventricular injection of the C60 hydrated fullerene at a dose of 7.2 nmol/ventricle significantly improved the performance of the cognitive task in control rats. The intracerebroventricular injection of the C60 hydrated fullerene (3.6 nmol/ventricle) prevented the impairment of performance of the cognitive task induced by amyloid-β25-35 (22.5 nmol/ventricle). The results obtained may be useful in the development of therapy of Alzheimer's disease.Journal of Nanoscience and Nanotechnology 03/2007; 7(4-5):1479-1485. · 1.56 Impact Factor -
Article: Study of the cytotoxicity of protein X amyloid fibrils
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ABSTRACT: Amyloid oligomers, protofibrils, and fibrils of various amyloidogenic proteins are known to induce cell death. Tetracycline prevents the formation of fibrils of Aβ peptide and other amyloidogenic proteins and decomposes mature fibrils. It was previously shown that sarcomeric cytoskeletal proteins of the titin family (protein X, protein C, and protein H) in vitro form amyloid fibrils and tetracycline decomposes them. In this work, the concentration and time dependence of the survival of polymorphonuclear leukocytes in the presence of protein X amyloid fibrils is demonstrated. It is also shown that the survival rate increases as fibrils are decomposed by tetracycline. The antibiotic itself is found to be nontoxic. The results obtained show that this approach can be used to evaluate the efficiency of drugs that prevent or rectify amyloidoses.Biophysics 09/2006; 51(5):705-709. -
Article: Electron microscopic study of the effect of fullerene on the formation of amyloid fibrils by the Aβ25–35 peptide
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ABSTRACT: The antiamyloidogenic effect of hydrated fullerence C60 HyFn was shown by electron microscopy. It was found that fullerene binds to growing fibrils formed by the [beta]-amyloid peptide Aβ25–35 and thus prevents their further growth and interferes with the formation of new fibrils. Instead of long broad helically twisted ‘ribbons’ formed by Aβ25–35 in the absence of fullerene, short narrow protofibrils form in its presence. These results suggest that fullerenes can be useful in treatment for Alzheimer’s disease.Biophysics 01/2006; 51(5):701-704. -
Article: Comparative electron microscopic study on projectin and titin binding to F-actin.
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ABSTRACT: Using the system of F-actin paracrystals, we have obtained electron microscopic evidence that projectin from synchronous flight muscles of Locusta migratoria binds to actin filaments in the same fashion as skeletal titin. Control actin paracrystals formed in the presence of Mg(2+) ions have great width and length and blunted ends. The addition of either projectin or titin results in disruption of compact ordered packing of F-actin in paracrystals and leads to the formation of loose filament bundles with smaller diameters and tapered ends. It is also accompanied with the appearance of individual actin filaments in considerable amounts. The effect becomes more pronounced with the increase in concentrations of added projectin or titin. Possible physiological implications of projectin-actin interactions are discussed.Insect Biochemistry and Molecular Biology 09/2003; 33(8):789-93. · 3.25 Impact Factor -
Article: [The use of cryomethods for research on the sarcomere ultrastructure of rabbit skeletal muscles].
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ABSTRACT: The ultrastructure of sarcomeres of glycerinated rabbit psoas muscle was studied using freeze-fracture-etching, freeze-drying and optical diffraction techniques in comparison with the investigation of this muscle by plastic sections and negative staining methods. In frozen and dried myofibrils isolated from the above muscle the stripes of minor proteins location in A- and I-disks were clearly seen. The pivot structure in thick filaments was revealed in longitudinal fractures of the muscle. The ordered arrangement of myosin heads (crossbridges) associated with actin filaments was preserved in frozen longitudinal fractures as evidenced by optical diffraction. Freeze etching technique allowed to revealed some details of Z-line structure: alpha-actinin bridges connecting the ends of actin filaments of neighbouring sarcomeres and to preserve the lateral struts between actin filaments in I-disks.Tsitologiia 02/1990; 32(11):1073-7. -
Article: Manifestation of the stripes of minor proteins location in A-bands of rabbit cardiac myofibrils.
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ABSTRACT: Cardiac myofibrils were isolated from rabbit ventricular muscle by a method that preserves well the integrity of the A-band structure. For the first time electron microscopic observations using the negative staining method revealed, in cardiac A-bands, a full complement of pronounced transverse stripes which indicate the locations of minor proteins in skeletal muscles. The manifestation of some transverse stripes in the cardiac A-band was shown to depend on the duration of muscle incubation in a Ca2(+)-depleting and ATP-free solution before its homogenization into myofibrils. The clear visibility of fine structural details in electron micrographs allowed us to resolve morphological features specific for cardiac muscle at both the central and end parts of the A-bands. The myofibrils demonstrated here are expected to be useful for elucidating the fine structure of cardiac thick filaments and in particular the locations of minor proteins.Journal of Molecular Biology 01/1990; 210(3):655-8. · 4.00 Impact Factor -
Article: Localization of binding sites of F-protein (phosphofructokinase) on the myosin molecule.
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ABSTRACT: To determine the location of F-protein binding sites on myosin, the interaction of F-protein with myosin and its proteolytic fragments in 0.1 M KCl, 10 mM potassium phosphate, pH 6.5, has been investigated using sedimentation, electron microscopy and optical diffraction methods. Sedimentation experiments show that F-protein can bind to myosin and myosin rod rather than to light meromyosin or subfragment-1. The F-protein binding to myosin and rod is of a similar character. The calculated values of the constants of F-protein binding to myosin and rod are 2.6 X 10(5) M-1 and 2.1 X 10(5) M-1, respectively. The binding sites are probably located on the subfragment-2 portion of the myosin molecule. The number of the F-protein binding sites calculated per chain weight of 80,000 is 5 +/- 1. Electron microscopic observations confirm the sedimentation results. F-protein does not bind to light meromyosin paracrystals, but decorates myosin and rod filaments with the interval of 14.3 nm regardless of whether F-protein is added prior to or after filamentogenesis. The comparison of optical diffraction patterns obtained from myosin and rod filaments with those from decorated ones reveals the marked enhancement of meridional reflection at (14.3 nm)-1 in the latter case. Neither the increase in ionic strength from 0.1 to 0.15 and pH from 6.5 to 7.3 nor substitution of potassium phosphate buffer by imidazole-HCl buffer, or Tris-HCl influences F-protein binding to myosin and rod filaments as visualized by electron microscopy. The possible significance of F-protein location in the thick filament structure is discussed.Journal of Muscle Research and Cell Motility 01/1987; 7(6):481-90. · 1.98 Impact Factor -
Article: [Determination of quantitative parameters of the binding of F-protein (phosphofructokinase) with myosin and localization of binding sites on the myosin molecule].
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ABSTRACT: To determine the localization of F-protein binding sites on myosin, the interaction of F-protein with myosin and its proteolytic fragments in 0.1 M KCl, 10 mM K-phosphate pH 6.5 was studied, using sedimentation, electron microscopic and optical diffraction methods. Sedimentation experiments showed that F-protein binds to myosin and myosin rod rather than to light meromyosin or S-1. The F-protein binding to myosin and rod is of a similar character. The calculated values of the constants of F-protein binding to myosin and rod are 2.6 X 10(5) M-1 and 2.1 X 10(5) M-1, respectively. The binding sites are probably located on the subfragment-2 portion of the myosin molecule. The number of F-protein binding sites on myosin calculated per chain weight of 80 000 is 5 +/- 1. The sedimentation results were confirmed by electron microscopic data. F-protein does not bind to light meromyosin paracrystals, but decorates myosin and rod filaments with the interval of 14.3 nm regardless of whether F-protein is added before or after filamentogenesis. A comparison of optical diffraction patterns obtained from myosin and rod filaments with those from decorated ones revealed a marked enhancement of meridional reflection at (14.3 nm)-1 in the latter case.Biokhimii͡a (Moscow, Russia) 11/1986; 51(10):1718-25. -
Article: Study on structural elements of sarcomere: structure of thick filaments in muscle and after isolation.
Acta histochemica. Supplementband 02/1981; 23:83-7. -
Article: [Electron microscopic study of the influence of fullerene on the formation of amyloid fibrils by A beta(25-35) peptide].
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ABSTRACT: The anti-amyloidogenic capacity of hydrated fullerene C60 HyFn was revealed by the use of electron microscopy. We first showed that when, connecting with growing amyloid fibrils formed by A beta(25-35)-peptide, fullerene prevented their subsequent growth and interfered with the formation of new fibrils. Instead of long helically twisted ribbons formed by A beta(25-35)-peptide in the absence of fullerene, short narrow protofibrils were found in the presence of fullerene . These results allow one to suppose that fullerene can be useful for the therapy of Alzheimer's disease.Biofizika 51(5):795-8. · 0.43 Impact Factor -
Article: Effects of hydrated forms of C60 fullerene on amyloid 1-peptide fibrillization in vitro and performance of the cognitive task.
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ABSTRACT: It has been shown for the first time by transmission electron microscopy that the hydrated fullerene C60 inhibited the fibrillization of amyloid-beta25-35 peptide. The fullerene affected the amyloid-beta25-35 assembly, manifesting its anti-amyloidogenic capacity. Our in vivo investigations demonstrated also that a single intracerebroventricular injection of the C60 hydrated fullerene at a dose of 7.2 nmol/ventricle significantly improved the performance of the cognitive task in control rats. The intracerebroventricular injection of the C60 hydrated fullerene (3.6 nmol/ventricle) prevented the impairment of performance of the cognitive task induced by amyloid-beta25-35 (22.5 nmol/ventricle). The results obtained may be useful in the development of therapy of Alzheimer's disease.Journal of Nanoscience and Nanotechnology 7(4-5):1479-85. · 1.56 Impact Factor -
Article: [Thick filament structure in isolated A-discs and longitudinal sections of vertebrate skeletal muscle by optical diffraction].
Biofizika 28(2):306-10. · 0.43 Impact Factor -
Article: [Sarcomeric proteins of the titin family form amyloids].
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ABSTRACT: It was shown for the first time that skeletal muscle sarcomeric proteins of the titin family (X-, C- and H-proteins) are able to form in vitro amyloid aggregates of different types: granular aggregates, protofibrils, helically twisted ribbons, linear fibrils, and bundles of linear fibrils. Their amyloid nature was confirmed by electron, polarization, and fluorescence microscopy and by spectral methods. As opposed to other amyloidogenic proteins, X-, C-, and H-proteins easily form amyloids under mild conditions close to physiological ones (pH, ionic strength, temperature). Like amyloid fibrils of Abeta-peptide and tau protein in Alzheimer's disease, amyloid aggregates formed by X-, C-, and H-proteins are destroyed by the antibiotic tetracycline. Thus, new proteins-precursors of amyloids and possible participants of amyloidoses in muscles were discovered. Further study of in vitro amyloidogenesis of these proteins would help to find approaches to controlling this process in organs and tissues.Biofizika 50(5):803-9. · 0.43 Impact Factor -
Article: [Structural changes in actin filaments during binding with phosphofructokinase (F-protein), detected using an optical diffraction method].
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ABSTRACT: It has been previously shown by us that phosphofructokinase (F-protein) binds to rabbit skeletal muscle F-actin and reconstituted thin filaments forming ordered bundles. Upon low molar ratios of phosphofructokinase to actin in the complex bundles the enzyme molecules are arranged between actin filaments regularly in the form of crossbridges. The increase of phosphofructokinase: actin ratio up to equimolar one and more leads to the filling of the space between actin filaments with phosphofructokinase-molecules. In these bundles the inclined cross striation with axial repeat of about 7.0 nm is clearly seen. Optical diffraction analysis of the micrographs revealed new features in the structure of such complexes in comparison with F-actin and reconstituted thin filaments. Optical diffraction patterns from F-actin and reconstituted thin filaments exhibit the first (35.5 nm) and sixth (5.9 nm) layer lines typical of F-actin helix structure. On the optical diffraction patterns from the complex bundles the meridional reflection of about 7.2nm is present, that is not observed on the optical diffraction patterns from F-actin alone. This reflection is characteristic of optical diffraction- and X-ray patterns of myosin filaments and is the sixth order of axial period of their structure (42.9 nm/6). The structural changes occurring upon binding is supposed to be important for the mutual regulation of functional activity of enzymes and actin-containing filaments in muscle.Biofizika 41(1):73-7. · 0.43 Impact Factor -
Article: [Changes in structural organization of reorganized myosin filaments from skeletal muscles of the winter-hibernating suslik Citellus undulatus during awakening].
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ABSTRACT: Electron microscopic observations of myosins, isolated from skeletal muscles of ground squirrels at the different states (hybernation-Mhyb; winter activity--M(act); the beginning of arousal-M(begin); the end of arousal-M(end)) have revealed the differences in the structure of their filaments. The filaments reconstituted from Mhyb, M(act) and M(end) (rectal temperature 27 degrees C) exhibit the ordered structure with the myosin heads arranged regularly (period of 14.3 nm) on the filament surface. The filaments of M(begin) (rectal temperature 12 degrees C) have irregular structure with random arrangement of the myosin head clusters alternating with regions of different length devoid of heads. To elucidate the molecular bases of the structural differences observed by us the solubility of the above myosins, the paracrystal structure of corresponding light meromyosins as well as the composition and state of myosin light chains have been studied. M(begin) appeared to have the greatest solubility in comparison with the other ones. This points to the possible changes in myosin heavy chains during hybernation and arousal although no differences have been revealed in paracrystals of light meromyosins. The amount of light chains (LC3) decreased up to 40% in Mhyb in comparison with Mact and then increased up to 60-70% in Mbegin within 1-1.5 hours of arousal. It is supposed that the rapid changes in isoforms of heavy and light chains in Mbegin lead to disturbance of regular mode of filament assembly in vitro, and can result in the lowering of muscle contraction efficiency and in the increasing of the heat release. The later can contribute to thermogenesis of the animal during arousal.Biofizika 41(1):116-22. · 0.43 Impact Factor -
Article: [Electron microscopic study of the shape and dimensions of F-protein].
Biofizika 26(4):761-3. · 0.43 Impact Factor
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- Biofizika (13)
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Institutions
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2006–2012
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Institute of Theoretical and Experimental Biophysics
Pushchino, Moskovskaya, Russia
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