Hsin-Ming Chen

National Taiwan University, T’ai-pei, Taipei, Taiwan

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Publications (76)124.59 Total impact

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    ABSTRACT: A portion of patients with recurrent aphthous stomatitis (RAS) may have nutritional deficiency. This study evaluated whether there was an intimate association of the deficiencies of hemoglobin, iron, vitamin B12, and folic acid and high blood homocysteine level with RAS.
    Journal of Oral Pathology and Medicine 07/2014; · 2.06 Impact Factor
  • Journal of dental sciences 06/2014; · 0.35 Impact Factor
  • Journal of the Formosan Medical Association 04/2014; · 1.00 Impact Factor
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    ABSTRACT: Perineural invasion (PNI) and nerve growth factor (NGF) expression are found to be significantly associated with the progression and/or prognosis of several human cancers. Immunohistochemical staining for S-100 and NGF proteins was performed to assess the PNI and NGF expression level in 116 oral tongue squamous cell carcinoma (OTSCC) specimens, respectively. The PNI rate increased from 22% of the original pathological report, through 39% after reevaluation of hematoxylin and eosin-stained tissue sections, to 51% with the help of anti-S-100 immunostaining. The positive PNI was significantly associated with larger tumor size (P = 0.033), positive lymph node metastasis (P = 0.001), advanced clinical stage (P < 0.001), greater tumor thickness (P < 0.001), close or positive section margin (P = 0.013), and higher grade of cancer invasion front pattern (P < 0.001). Moreover, the high NGF expression level was significantly correlated with larger tumor size (P = 0.009), positive lymph node metastasis (P = 0.004), advanced clinical stage (P < 0.001), greater tumor thickness (P = 0.005), close or positive section margin (P = 0.030), and the positive PNI (P = 0.009). In addition, OTSCC patients with positive PNI or high NGF expression level had significantly worse overall survival than those with negative PNI or low NGF expression level, respectively. Anti-S-100 immunostaining is an effective technique to detect occult PNI. Both the positive PNI and NGF expression level are valuable biomarkers that can predict the progression of OTSCC and prognosis of OTSCC patients.
    Journal of Oral Pathology and Medicine 04/2014; 43(4):258-64. · 2.06 Impact Factor
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    ABSTRACT: Background. Early growth response-1 (Egr-1) protein plays an important role in many human fibrotic diseases. Areca nut (AN) chewing is the most important risk factor of oral submucous fibrosis (OSF). Methods. Egr-1 protein expression in OSF was examined using antibody to Egr-1. Arecoline-induced Egr-1 expression and its signaling pathways were assessed by Western blot analyses in human buccal mucosal fibroblasts (BMFs). Results. Elevated Egr-1 staining was observed in epithelial cells, fibroblast and inflammatory cells in 7 of 10 OSF cases. Arecoline, a main alkaloid found in AN, stimulated Egr-1 synthesis in BMFs. Pretreatment with antioxidant N-acetyl-L-cysteine, JNK inhibitor SP600125 and ERK inhibitor PD98059 significantly reduced arecoline-induced Egr-1 synthesis. Epigallocatechin-3-gallate (EGCG) inhibited arecoline-induced Egr-1 synthesis and collagen gel contraction in a dose-responsive manner. Conclusion. Constitutive Egr-1 expression during AN chewing may play a role in the pathogenesis of OSF. EGCG could be a good candidate for prevention or treatment of OSF. Head Neck, 2014.
    Head & Neck 01/2014; · 2.83 Impact Factor
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    ABSTRACT: Background/purpose Dental patients with mental, psychiatric, or neurological disorders may need general anesthesia during dental treatments. This study analyzed the dental treatments and postoperative complications of 200 dental patients in a special needs dental clinic in northern Taiwan. Materials and methods The dental treatments and postoperative complications of 200 dental patients who needed general anesthesia for performing the dental treatments in a special needs dental clinic were analyzed statistically. Results The mean age of 200 dental patients (97 pediatric and 103 adult patients; 130 males and 70 females) was 20.2 ± 15.2 years. Of the 200 patients, 145 had tooth extractions, 118 tooth fillings, 46 root canal treatments, 56 either pulpotomy or pulpectomy, 45 stainless steel crown placement, and seven panoramic radiographic examinations. In pediatric patients, psychiatric problems were the most commonly encountered disorders (61.9%), followed by mental retardation (44.3%). In adult patients, mental retardation was the most common disorder (81.6%), followed by psychiatric disorders (34.0%). Of the 200 patients, 66 needed intubation general anesthesia (IGA) and 134 nonintubation general anesthesia (NIGA). IGA patients needed a significantly longer operation duration than NIGA patients (P < 0.001). Regarding postoperative complications, IGA patients had significantly higher epistaxis episodes than NIGA patients (P < 0.001). However, NIGA patients had significantly higher desaturation rate than IGA patients (P = 0.028). Conclusion Both IGA and NIGA are effective and relatively safe methods for dental patients who need dental treatment in a special needs dental clinic, but anesthesia itself still carries certain risks.
    Journal of Dental Sciences. 01/2014;
  • Journal of Dental Sciences. 01/2014;
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    ABSTRACT: Background/Purpose Autoimmune thyroiditis can be diagnosed by measuring patients' serum levels of thyroid stimulating hormone (TSH), anti-thyroglobulin antibody (TGA), and anti-thyroid microsomal antibody (TMA). This study evaluated whether there were hematinic deficiencies, high blood homocysteine levels, and serum gastric parietal cell antibody (GPCA) positivity in patients with TGA or TMA. Methods The blood hemoglobin (Hb), iron, vitamin B12, folic acid, homocysteine and TSH concentrations and the serum GPCA level in 190 TGA- or TMA-positive patients were measured and compared with the corresponding levels in 190 age- and sex-matched healthy control subjects. Results We found that 31 (16.3%), 27 (14.2%), 12 (6.3%), and 2 (1.1%) TGA- or TMA-positive patients had deficiencies of Hb (Men < 13 g/dL, Women < 12 g/dL), iron (< 60 μg/dL), vitamin B12 (< 200 pg/mL), and folic acid (< 4 ng/mL), respectively. Moreover, 25 (13.2%) and 48 (25.3%) TGA- or TMA-positive patients had abnormally high blood homocysteine level and serum GPCA positivity, respectively. TGA- or TMA-positive patients had a significantly higher frequency of Hb (p < 0.001), iron (p < 0.001), or vitamin B12 deficiency (p = 0.001), of abnormally elevated blood homocysteine level (p = 0.001), or of serum GPCA positivity (p < 0.001) than healthy control subjects. Of 190 TGA- or TMA-positive patients, 8 (4.2%) had lower serum TSH level (< 0.1 μIU/mL, suggestive of hyperthyroidism), 163 (85.8%) had serum TSH level within normal range (0.1–4.5 μIU/mL), and 19 (10%) had higher serum TSH level (>4.5 μIU/mL, suggestive of hypothyroidis). Conclusion There are significant deficiencies of hemoglobin, iron, and vitamin B12, abnormally high blood homocysteine levels, and serum GPCA positivity in TGA- or TMA-positive patients. In addition, the majority (85.8%) of TGA- or TMA-positive patients had euthyroid and only a small portion (14.2%) of TGA- or TMA-positive patients had either hypothyroidism or hyperthyroidism.
    Journal of the Formosan Medical Association 01/2014; 113(3):155–160. · 1.00 Impact Factor
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    ABSTRACT: Background/Purpose Anxiety induced by dental treatment can become a serious problem, especially for patients with special needs. Application of deep touch pressure, which is a sensory adaptation technique, may ameliorate anxiety in disabled patients. However, few empiric studies have investigated the possible links between the clinical effects of deep touch pressure and its behavioral and physiologic aspects. Equally little progress has been made concerning theoretical development. The current study is a crossover intervention trial to investigate the behavioral and physiological effects of deep touch pressure for participants receiving dental treatment. Methods Nineteen disabled participants, who were retrospectively subclassified for positive trend or negative trend, were recruited to receive the papoose board as an application of deep touch pressure. Quantitative analyses of behavioral assessments and physiological measurements, including electrodermal activity and heart rate variability, were conducted. We sought to understand the modulation of the autonomic nervous system and the orchestration of sympathetic and parasympathetic (PsNS) nervous systems. Results Behavioral assessments reported that higher levels of anxiety were induced by the dental treatment for participants with both groups of positive and negative trends. Although no significant differences were found in the SNS activity, physiologic responses indicated that significantly changes of PsNS activity were observed under the stress condition (dental treatment) when deep touch pressure intervention was applied, especially for participants in the group of positive trend. Conclusion Our results suggest that the PsNS activation plays a critical role in the process of ANS modulation. This study provides not only physiologic evidence for the modulation effects of deep touch pressure on stressful conditions in dental environments but also the evidence that the application of papoose board, as a sensory adaptation technique, is not harmful for dental patients with special needs.
    Journal of the Formosan Medical Association 01/2014; 113(2):94–101. · 1.00 Impact Factor
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    ABSTRACT: Previous studies have used cryotherapy for the treatment of oral precancers including oral leukoplakia (OL) and oral verrucous hyperplasia (OVH) as well as oral cancers including oral verrucous carcinoma (OVC) and oral squamous cell carcinoma (OSCC). Cryotherapy is a method that locally destroys lesional tissues by freezing in situ. It can be carried out by either an “open” or a “closed” system. Lesional tissues are destroyed mainly through disruption of cell membrane, cellular dehydration, enzyme and protein damage, cell swelling and rupture, thermal shock injury to cells, damage to vasculature, and immune-mediated cytotoxicity. Cryotherapy is used frequently for the treatment of OL lesions with promising results. It can also be used to treat OVH and OVC lesions. Because OVH and OVC lesions are usually fungating and bulky, a combination therapy of shave excision and cryotherapy is needed to achieve a complete regression of the lesion. OSCCs have also been treated by cryotherapy. However, cryotherapy is not the main-stream treatment modality for OSCCs. Cryotherapy seems suitable for treatment of thin or relatively thick plaque-typed lesions such as OL lesions. By careful selection of patients, cryotherapy is a simple, safe, easy, conservative, and acceptable treatment modality for certain benign oral lesions and oral precancers.
    Journal of the Formosan Medical Association 01/2014; · 1.00 Impact Factor
  • Journal of the Formosan Medical Association. 01/2014;
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    ABSTRACT: Langerhans cell (LC) is an antigen-presenting cell that is very important for T-cell-mediated immune reactions. Our previous studies have shown the presence of LCs in some odontogenic tumors and cysts. In this study, we further examined the presence of LCs in odontogenic epithelia of 16 odontogenic fibromas (OFs). Anti-CD1a and anti-S-100 immunostains were used to detect the presence of LCs in nests or strands of odontogenic epithelia of 16 OFs. These 16 OFs included 10 peripheral OFs excised from seven male and three female patients (mean age, 38 years) and six central OFs (including one recurrent OF) removed from five male patients (mean age, 28 years). Of the 10 peripheral OFs, six were found on the mandibular gingiva and four on the maxillary gingiva. Four central OFs were located in the maxilla and two in the mandible. We found that both anti-CD1a and anti-S-100 immunostains had an equal ability to identify LCs in OFs. Positively stained dendritic LCs could be detected in nests and strands of odontogenic epithelia in nine (six peripheral and three central OFs) of the 16 OFs. In five peripheral OFs, dendritic LCs were found in occasional nests or strands of odontogenic epithelia. In one peripheral and three central OFs, dendritic LCs could be detected in at least half of the nests or strands of odontogenic epithelium in the tissue section. LCs can be detected in the nests or strands of odontogenic epithelia in approximately 60% of the 10 peripheral OFs and approximately 50% of the six central OFs detected.
    Journal of the Formosan Medical Association 12/2013; · 1.00 Impact Factor
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    ABSTRACT: Overexpression of MCM5 protein has been found to be significantly associated with the progression and prognosis of several human cancers. This study used immunohistochemistry to examine the expression of MCM5 protein in 97 specimens of oral squamous cell carcinomas (OSCC), 80 specimens of oral epithelial dysplasia (OED, including 31 mild, 29 moderate, and 20 severe OED samples), and 20 specimens of normal oral mucosa (NOM). We found that the mean nuclear MCM5 labeling indices (LIs) increased significantly from NOM (15 ± 6%), through mild (25 ± 10%), moderate (34 ± 9%), and severe OED (43 ± 12%), to OSCC samples (61 ± 16%, P < 0.001). A significant correlation was found between the higher mean nuclear MCM5 LI and OSCCs with site at the tongue (P = 0.046), larger tumor size (P = 0.032), positive lymph node metastasis (P = 0.003), more advanced clinical stage (P = 0.002), higher histological grade (P = 0.002), deeper invasion depth (P = 0.0001), and perineural invasion (P = 0.0047). Only nuclear MCM5 LI ≧ 60% was identified as independent unfavorable prognostic factor by multivariate regression analyses (P = 0.049). The Kaplan-Meier curve showed that patients with OSCC with a nuclear MCM5 LI ≧ 60% had a significantly poorer cumulative survival than those with a nuclear MCM5 LI < 60% (log-rank test, P = 0.0062). The increased expression of MCM5 protein begins at the oral pre-cancerous stage. The higher expression of MCM5 protein is significantly associated with the aggressive progression and poor prognosis of OSCC.
    Journal of Oral Pathology and Medicine 11/2013; · 2.06 Impact Factor
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    ABSTRACT: Traumatic ulcerative granuloma with stromal eosinophilia (TUGSE) is a special oral ulcerative lesion that shares many clinical features of an oral squamous cell carcinoma. This study reports the clinicopathological features of 34 oral TUGSE lesions in Taiwanese patients. Thirty-four TUGSE cases were retrieved from the files of the Department of Oral Pathology and Oral Diagnosis, National Taiwan University Hospital from 2003 to 2009. Their clinical data and histopathological features were examined, collected, and analyzed. The study group included 22 male and 12 female patients (64.7% and 35.3%, respectively) with oral TUGSE. The mean age of the patients was 49 years (range, 8 to 80 years). The most common site for oral TUGSE lesions was the tongue (23 cases, 67.6%), followed by the buccal mucosa (6 cases, 17.6%), retromolar area (2 cases, 5.9%), floor of the mouth and lingual sulcus (2 cases, 5.9%), and lip (1 case, 3.0%). For 23 tongue cases, 19 occurred on the dorsum and the tip (82.6%) and 4 on the ventral surface (17.4%). Of the 34 oral TUGSE lesions, 13 (38.2%) had a mild, 11 (32.4%) a moderate, and 10 (29.4%) a severe eosinophilic infiltrate. Oral TUGSE lesions occur more frequently on the dorsal surface and the tip of the tongue and in male patients between 41 and 60 years of age. The eosinophilic infiltrates in oral TUGSE lesions show a scattered or clustered pattern, and their density varies from case to case. Oral TUGSE is a self-limiting lesion, and aggressive surgical treatment is usually not required.
    Journal of the Formosan Medical Association 10/2013; · 1.00 Impact Factor
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    ABSTRACT: Patients with thalassemia trait (TT) may have anemia. This study evaluated whether TT patients had specific oral manifestations and a particular blood profile compared with normal individuals. The oral manifestations and mean red blood cell count, corpuscular cell volume, red blood cell distribution width, Mentzer index, and Green and King index as well as blood concentrations of hemoglobin, iron, total iron binding capacity, vitamin B12, folic acid, and homocysteine in 65 TT patients and in 130 age- and sex-matched healthy controls were measured and compared. TT patients had significantly higher frequencies of all oral manifestations than healthy controls (p < 0.001 for all), in which burning sensation of oral mucosa (90.8%), lingual varicosity (90.8%), dry mouth (72.3%), atrophic glossitis (32.3%), and numbness of the oral mucosa (30.8%) were the five leading oral manifestations for TT patients. Moreover, TT patients had significantly lower mean hemoglobin level, corpuscular cell volume, Mentzer index, and Green and King index (p < 0.001 for all) as well as significantly higher mean red blood cell count and red blood cell distribution width (p < 0.001 for both) than healthy controls. However, no significant difference in the mean blood iron, total iron binding capacity, vitamin B12, folic acid, or homocysteine levels was discovered between 65 TT patients and 130 healthy controls. TT patients have specific oral manifestations and a particular blood profile compared to normal individuals.
    Journal of the Formosan Medical Association 10/2013; · 1.00 Impact Factor
  • Hsin-Ming Chen
    Journal of the Formosan Medical Association 09/2013; · 1.00 Impact Factor
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    ABSTRACT: Connective tissue growth factor (CCN2) has been associated with the pathogenesis of various fibrotic diseases, including oral submucous fibrosis (OSF). The chemical constituents of areca nut along with the mechanical trauma cause OSF. The coarse fibers of areca nut injure the mucosa and hence sphingosine-1-phosphate (S1P) is released at the wounded sites. Recent studies have shown that S1P is involved in wound healing and the development of fibrosis. The aims of this study were to investigate the effects of S1P on CCN2 expression in human buccal fibroblasts (HBFs) and identify the potential targets for drug intervention or chemoprevention of OSF. Western blot analyses were used to study the effects of S1P on CCN2 expression and its signaling pathways in HBFs and whether epigallocatechin-3-gallate (EGCG), the main and most significant polyphenol in green tea, could inhibit this pathway. S1P significantly enhanced CCN2 synthesis in HBFs. This effect can be inhibited by c-Jun NH2-terminal kinase (JNK) inhibitor and extracellular signal-regulated kinase inhibitor but not by P38 mitogen-activated protein kinase inhibitor. Interestingly, EGCG completely blocked S1P-induced CCN2 expression via suppressing S1P-induced JNK phosphorylation. S1P released by repetitive mechanical trauma during AN chewing may contribute to the pathogenesis of OSF through upregulating CCN2 expression in HBFs. EGCG could be an adjuvant to the current offered therapy options or the prevention of OSF through suppression of JNK activation.
    Journal of the Formosan Medical Association 09/2013; · 1.00 Impact Factor
  • Chun-Pin Chiang, Hsiang Yang, Hsin-Ming Chen
    Journal of the Formosan Medical Association 07/2013; · 1.00 Impact Factor
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    ABSTRACT: Burning mouth syndrome (BMS) is characterized by the presence of burning sensation of the oral mucosa in the absence of clinically apparent mucosal alterations. It occurs more commonly in middle-aged and elderly women and often affects the tongue tip and lateral borders, lips, and hard and soft palate. In addition to a burning sensation, the patients with BMS may also complain unremitting oral mucosal pain, dysgeusia, and xerostomia. BMS can be classified into two clinical forms: primary and secondary BMS. The primary BMS is essential or idiopathic, in which the organic local/systemic causes cannot be identified and a neuropathological cause is likely. The diagnosis of primary BMS depends mainly on exclusion of etiological factors. The secondary BMS is caused by local, systemic, and/or psychological factors; thus, its diagnosis depends on identification of the exact causative factor. When local, systemic or psychological factors are present, treatment or elimination of these factors usually results in a significant clinical improvement of BMS symptoms. Vitamin, zinc, or hormone replacement therapy has been found to be effective for reducing the oral burning or pain symptom in some BMS patients with deficiency of the corresponding factor. If patients still have the symptoms after the removal of potential causes, drug therapy should be instituted. Previous randomized controlled clinical trials found that drug therapy with capsaicin, alpha-lipoic acid, clonazepam, and antidepressants may provide relief of oral burning or pain symptom. In addition, psychotherapy and behavioral feedback may also help eliminate the BMS symptoms.
    Journal of Oral Pathology and Medicine 06/2013; · 2.06 Impact Factor
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    ABSTRACT: Burning mouth syndrome (BMS) is characterized by a burning sensation of the oral mucosa in the absence of clinically apparent mucosal alterations. In this study, we evaluated whether there was an intimate association of the deficiency of hemoglobin (Hb), iron, vitamin B12, or folic acid; high blood homocysteine level; and serum gastric parietal cell antibody (GPCA) positivity with BMS. Blood Hb, iron, vitamin B12, folic acid, and homocysteine concentrations and the serum GPCA level were measured in 399 BMS patients and compared with the corresponding levels in 399 age- and sex-matched healthy control individuals. We found that 89 (22.3%), 81 (20.3%), 10 (2.5%), and six (1.5%) BMS patients had deficiencies of Hb (men: <13 g/dL, women: <12 g/dL), iron (<60 μg/dL), vitamin B12 (<200 pg/mL), and folic acid (<4 ng/mL), respectively. Moreover, 89 (22.3%) BMS patients had abnormally high blood homocysteine level and 53 (13.3%) had serum GPCA positivity. BMS patients had a significantly higher frequency of Hb, iron, or vitamin B12 deficiency; of abnormally elevated blood homocysteine level; or of serum GPCA positivity than the healthy control group (all p < 0.001 except for vitamin B12 deficiency, for which p = 0.004). However, no significant difference in frequency of folic acid deficiency (p = 0.129) was found between BMS patients and healthy control individuals. We conclude that there is a significant association of deficiency of Hb, iron, and vitamin B12; abnormally high blood homocysteine level; and serum GPCA positivity with BMS.
    Journal of the Formosan Medical Association 06/2013; 112(6):319-325. · 1.00 Impact Factor

Publication Stats

486 Citations
124.59 Total Impact Points

Institutions

  • 2003–2014
    • National Taiwan University
      • • Department of Dentistry
      • • Graduate Institute of Clinical Dentistry
      • • College of Medicine
      T’ai-pei, Taipei, Taiwan
    • National Taiwan University Hospital
      • School of Dentistry
      T’ai-pei, Taipei, Taiwan
    • I-Shou University
      Kao-hsiung-shih, Kaohsiung, Taiwan
  • 2003–2013
    • Taipei Medical University
      • Graduate Institute of Biomedical Materials
      T’ai-pei, Taipei, Taiwan
  • 2012
    • Chung Shan Medical University
      臺中市, Taiwan, Taiwan
  • 2008
    • Harvard Medical School
      Boston, Massachusetts, United States