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ABSTRACT: Cobalt oxide sensing film was in situ prepared on glassy carbon electrode surface via constant potential oxidation. Controlling at 0.8 V in NaOH solution, the high-valence cobalt catalytically oxidized the reduced compounds, decreasing its surface amount and current signal. The current decline was used as the response signal of chemical oxygen demand (COD) because COD represents the summation of reduced compounds in water. The surface morphology and electrocatalytic activity of cobalt oxide were readily tuned by variation of deposition potential, time, medium and Co(2+) concentration. As confirmed from the atomic force microscopy measurements, the cobalt oxide film, that prepared at 1.3 V for 40 s in pH 4.6 acetate buffer containing 10 mM Co(NO(3))(2), possesses large surface roughness and numerous three-dimensional structures. Electrochemical tests indicated that the prepared cobalt oxide exhibited high electrocatalytic activity to the reduced compounds, accompanied with strong COD signal enhancement. As a result, a novel electrochemical sensor with high sensitivity, rapid response and operational simplicity was developed for COD. The detection limit was as low as 1.1 mg L(-1). The analytical application was studied using a large number of lake water samples, and the accuracy was tested by standard method.
Analytica chimica acta 07/2012; 736:55-61. · 4.31 Impact Factor
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ABSTRACT: This study investigated the conjoined cellular oxidative damage of human embryo kidney 293T (HEK293T) cells induced by cadmium
chloride (CdCl2) and nanometer titanium dioxide (nano-TiO2). RT-PCR technique was used to detect the expressions of Heme oxygenase-1 (HO-1) and 8-oxoguanine DNA glycosylase (OGG1). The activities of superoxide dismutase (SOD) and catalase enzyme (CAT) and concentrations of reactive oxygen species (ROS)
and maldondialdehyde (MDA) were measured by different approaches. The results showed that CdCl2 and nano-TiO2 at a low concentration of 0.75 total toxic unit (TU) exerted an additive effects on HO-1 gene expression, CAT activities and MDA concentrations. When the total TU was increased to 1 or 1.25 TU, the interaction
was synergetic. Moreover, the mixture with high proportion of CdCl2 produced an additive effect on the OGG1 gene expression, and the interaction was changed to be synergetic when the concentration of CdCl2 was lower than or equal to that of nano-TiO2. Synergetic effects of CdCl2 and nano-TiO2 on cellular oxidative damage of HEK293T cells were found as indicated by the changes in the SOD activities and ROS concentrations.
It was concluded that CdCl2 and nano-TiO2 exerts synergistic effects on the cellular oxidative damage of HEK293T cells, and the sensitivity of these indicators of
oxidative damage varies with the proportion of CdCl2 and nano-TiO2 in the mixture.
Key wordscadmium chloride–nano-TiO2
–oxidative damage–HEK293T cells
Journal of Huazhong University of Science and Technology 04/2012; 31(3):290-294. · 0.38 Impact Factor
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ABSTRACT: Mesoporous SiO2 was synthesized and used for modification of the surface of a carbon paste electrode (CPE). In pH 8.0 phosphate buffer, an
irreversible oxidation peak at 0.38V is observed for hesperidin. Compared to the unmodified CPE, the modified CPE remarkably
increases the oxidation signal of hesperidin. The effects of pH value, amount of mesoporous SiO2 and accumulation time were examined. As a result, a sensitive and convenient electroanalytical method was developed for hesperidin.
Its linear range is from 0.5 to 25μM, and the limit of detection is 0.25μM. The method was applied to determine hesperidin
in several traditional Chinese medicines.
Microchimica Acta 04/2012; 167(1):35-39. · 3.03 Impact Factor
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Analytical Methods. 03/2012; 4:1155-1159.
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ABSTRACT: Detection of analytes in complex biological samples, such as milk and blood, normally requires sample pretreatment. These pretreatment regimes reduce assay throughput and increase testing costs. Technologies that make it possible to eliminate sample pretreatment are of great industrial interest. Here we report the development of a dual-signal flow injected analysis device which eliminates the need for sample pretreatment. The device employs thermal traducers to measure the signal from an enzyme and a reference column. This makes it possible to independently monitor and correct for non-specifically generated heat, thereby eliminating the need for sample pretreatment. The ability of the dual-signal device to determine urea and lactate in milk samples without any prior treatment was evaluated. The spiked milk samples, the urea assay had a linear range from 0.1 to 50mM (R=0.996), and the lactate assay had a linear range from 0.025 to 5.0mM (R=0.9998). The linear regression values for urea and lactate for 0.5%, 1.5% and 3.0% fat milk were at least 0.990. The dual-signal design improves assay reproducibility, accuracy and sensitivity. Addition benefits are shorter assay times and lowers costs, as well as reducing equipment and training requirements. The potential application of the technology for multi-analyte analysis in point of care and decentralized diagnostic testing in healthcare, agriculture and environmental areas is discussed.
Biosensors & bioelectronics 08/2011; 29(1):115-8. · 5.43 Impact Factor
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ABSTRACT: A novel nitrite biosensor was developed through a sensing platform consisted of single-layer graphene nanoplatelet (SLGnP)-protein composite film. SLGnP with the virtues of excellent biocompatibility, conductivity and high sensitivity to the local perturbations can provide a biocompatible microenvironment for protein immobilization and a suitable electron transfer distance between electroactive centers of heme protein and electrode surface. A pair of well-defined and quasi-reversible cyclic voltammetric peaks that reflected the direct electrochemistry for ferric/ferrous couple of myoglobin (Mb) was achieved at the composite film modified electrode. Field emission scanning electron microscopy (FESEM) and ultraviolet visible spectra (UV-vis) were utilized to characterize the composite film. The results demonstrated that the morphology of the composite film was unique and the protein in the composite film retained its secondary structure similar to the native state. The composite film also displayed excellent electrocatalytic ability for the reduction of nitric oxide, which was applied to determine nitrite indirectly. It exhibited good electrochemical response to nitrite with a linear range from 0.05 to 2.5 mM and a detection limit of 0.01 mM.
Biosensors & bioelectronics 07/2011; 26(11):4436-41. · 5.43 Impact Factor
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ABSTRACT: A rapid, sensitive and selective electrochemical method was proposed for the determination of 2,4-dinitrophenol (2,4-DNP) in surface water samples, using hydrophilic molecular imprinted polymers (MIPs) as the recognition element and nickel (Ni) fiber as the catalytic element. Hydrophilic MIPs were synthesized using 2,4-DNP as the template, acrylamide as the monomer, glycidilmethacrylate as the pro-hydrophilic co-monomer and acetonitrile as the solvent. Hydrophilic modification could enhance the accessibility of 2,4-DNP to the imprinted cavities and improve the selective recognition properties of traditional MIPs in water medium. Subsequently, hydrophilic MIPs/Ni fiber electrode was prepared to determine trace 2,4-DNP by cyclic voltammetry. The parameters affecting the analytical performance were investigated. Under optimized conditions, the linear range was 0.7-30 μg L⁻¹ and the detection limit was 0.1 μg L⁻¹. Finally, the proposed method was applied to measure 2,4-DNP in surface water samples. The spiked recoveries were changed from 91.3% to 102.6% and the RSD was not higher than 5.1%. There was no statistically significant difference between the results obtained by the proposed method and the traditional chromatographic method.
Biosensors & bioelectronics 07/2011; 26(11):4450-6. · 5.43 Impact Factor
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ABSTRACT: An automated system has been developed for the determination of trace tetracycline antibiotics (TCs) in egg samples, based on online molecularly imprinted solid-phase extraction (MISPE) coupling with high-performance liquid chromatography (HPLC). Oxytetracycline and chlortetracycline were chosen as mixed templates to synthesize highly selective molecularly imprinted polymers for online extraction. Under the optimal online MISPE-HPLC condition, 10 mL egg samples were injected into the MISPE column and then the matrix was washed out. By rotating the switching valve, TCs were transferred to the analytical column and then separated by HPLC. Because sample pretreatment and chromatographic separation were carried out simultaneously, the whole analytical time (18 min) was significantly shortened compared with conventional offline techniques. The detection limits ranged from 0.8 to 1.3 ng/g. The enhancement factors were in the range of 159-410. The spiked recoveries of TCs in real egg samples ranged from 91.6 to 107.6% and the relative standard deviations (RSDs) were not higher than 4.0%.
Journal of Separation Science 06/2011; 34(12):1469-76. · 2.73 Impact Factor
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ABSTRACT: This study investigated the conjoined cellular oxidative damage of human embryo kidney 293T (HEK293T) cells induced by cadmium chloride (CdCl(2)) and nanometer titanium dioxide (nano-TiO(2)). RT-PCR technique was used to detect the expressions of Heme oxygenase-1 (HO-1) and 8-oxoguanine DNA glycosylase (OGG1). The activities of superoxide dismutase (SOD) and catalase enzyme (CAT) and concentrations of reactive oxygen species (ROS) and maldondialdehyde (MDA) were measured by different approaches. The results showed that CdCl(2) and nano-TiO(2) at a low concentration of 0.75 total toxic unit (TU) exerted an additive effects on HO-1 gene expression, CAT activities and MDA concentrations. When the total TU was increased to 1 or 1.25 TU, the interaction was synergetic. Moreover, the mixture with high proportion of CdCl(2) produced an additive effect on the OGG1 gene expression, and the interaction was changed to be synergetic when the concentration of CdCl(2) was lower than or equal to that of nano-TiO(2). Synergetic effects of CdCl(2) and nano-TiO(2) on cellular oxidative damage of HEK293T cells were found as indicated by the changes in the SOD activities and ROS concentrations. It was concluded that CdCl(2) and nano-TiO(2) exerts synergistic effects on the cellular oxidative damage of HEK293T cells, and the sensitivity of these indicators of oxidative damage varies with the proportion of CdCl(2) and nano-TiO(2) in the mixture.
Journal of Huazhong University of Science and Technology 06/2011; 31(3):290-4. · 0.38 Impact Factor
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ABSTRACT: Topotecan, a novel anticancer drug, has been widely used in the treatment of ovarian and cervical cancers. Herein, acetylene black (AB) nanoparticles were used to modify the electrode surface, and the electrochemical behavior of topotecan was examined. At the AB film surface, an irreversible oxidation peak was observed for topotecan, and the response signal greatly increased. The influences of pH value, amount of AB, accumulation potential and time were investigated. As a result, a novel electrochemical method with high sensitivity and rapid response was developed for the detection of topotecan. The linear range is from 2 μg L(-1) to 0.4 mg L(-1), and the limit of detection is 1.45 μg L(-1) (or 3.17×10(-9) M). The method was successfully used to detect topotecan in blood serum, and the results consisted with the values that obtained by high performance liquid chromatography.
Colloids and surfaces. B, Biointerfaces 05/2011; 84(1):135-9. · 2.60 Impact Factor
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Tao Jing,
Huan Xia,
Qin Guan,
Wenhong Lu,
Qing Dai,
Jiwei Niu,
Jae-Min Lim,
Qiaoling Hao,
Yong-Ill Lee, Yikai Zhou,
Surong Mei
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ABSTRACT: A rapid, low cost and selective chemiluminescence method coupled with magnetic molecularly imprinted polymers extraction was developed to detect lysozyme in human urine samples. Compared with traditional solid-phase extraction, this method could achieve selective extraction for the lysozyme, avoid the time consuming elution from a column or centrifugation steps, and then showed great potential in the high-throughput screening of clinical samples. The parameters affecting the performance of extraction and chemiluminescence were investigated. Under optimal conditions, the whole analytical procedure was completed within 12 min and spiked recovery ranged from 90.1% to 103.7% (R.S.D.≤6.7%). The limit of quantitation was 5 ng mL(-1). Furthermore, the results obtained by the proposed method were linearly correlated to those by commercial lysozyme detection kit (r=0.9595). Finally, the validated method was used to measure the urinary lysozyme of renal disease patients and healthy controls. The results confirmed the reliability and practicality of the protocol and revealed a good perspective of this method for biological sample analysis.
Analytica chimica acta 04/2011; 692(1-2):73-9. · 4.31 Impact Factor
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ABSTRACT: Titanium dioxide (TiO(2) ) is a widely used nanomaterial that can cause biological damage through oxidative stress. At low concentrations, TiO(2) can interact with lead acetate (PbAc) to produce different toxic responses, compared with TiO(2) or PbAc alone. In this study, we utilized the following as indicators of toxic responses in human embryo hepatocytes (L02): reactive oxygen species (ROS), reduced glutathione (GSH), superoxide dismutase (SOD), and the DNA adducts 8-hydroxydeoxyguanosine (8-OHdG) and 8-oxoguanine DNA glycosylase homolog 1 (OGG1). These were used to evaluate the oxidative stress of TiO(2) (at 0.001, 0.01, 0.1, 1, and 10 μg mL(-1) ) mixed with PbAc (1 μg mL(-1) ) on L02 cells without photoactivation. Compared with the negative control (1‰ dimethyl sulfoxide), TiO(2) mixed with PbAc induced increased release of ROS (at 0.001, 0.01, 0.1, 1, 10 μg mL(-1) TiO(2) ), intracellular SOD activity (at 0.1 and 0.01 μg mL(-1) TiO(2) ), GSH levels (at 0.01-1 μg mL(-1) TiO(2) ), 8-OHdG levels (at 1 and 10 μg mL(-1) TiO(2) ), OGG1 expression (at 0.001-1 μg mL(-1) TiO(2) ), and cytotoxicity (at 0.1, 1, and 10 μg mL(-1) TiO(2) ) in L02 cells. There were no significant changes in ROS, GSH, SOD, 8-OHdG, or OGG1 levels when L02 cells were treated with TiO(2) alone or PbAc alone. These findings indicate that TiO(2) and PbAc in combination induce cytotoxicity and oxidative stress in L02 cells in the absence of photoactivation. © 2011 Wiley Periodicals, Inc. Environ Toxicol, 2012.
Environmental Toxicology 01/2011; 27(10):590-7. · 2.41 Impact Factor
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ABSTRACT: Molecular imprinting is an attractive technique for preparing mimics of natural, biological receptors. Nevertheless, the imprinting of macromolecule remains a challenge due to their bulkiness and sensitivity to denaturation. In this work, we presented a method for preparing multifunctional lysozyme-imprinted nanoparticles (magnetic susceptibility, molecular recognition and environmental response). The magnetic susceptibility was imparted through the successful encapsulation of Fe3O4 nanoparticles. Selective lysozyme recognition depended on molecularly imprinted film. Moreover, it was also a hydrophilic stimuli-responsive polymer, which could undergo a reversible change of imprinted cavity in response to a small change in the environmental conditions. Thus, magnetic molecularly imprinted nanoparticles had high adsorption capacity (0.11 mg mg(-1)), controlled selectivity and direct magnetic separation (22.1 emicro g(-1)) in crude samples. After preconcentration and purification with magnetic MIPs nanoparticles, a sensitive chemiluminescence method was developed for determination of lysozyme in human serum samples. The results indicated that the spiked recoveries were changed from 92.5 to 113.7%, and the RSD was lower than 11.8%.
Biosensors & bioelectronics 10/2010; 26(2):301-6. · 5.43 Impact Factor
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ABSTRACT: Bacillary dysentery is still a common and serious public health problem in China. This paper is aimed at developing and evaluating an innovative hybrid model, which combines the seasonal autoregressive integrated moving average (SARIMA) and the generalized regression neural network (GRNN) models, for bacillary dysentery forecasting. Data of monthly bacillary dysentery incidence in Yichang City from 2000-2007 was obtained from Yichang Disease Control and Prevention Center. The SARIMA and SARIMA-GRNN model were developed and validated by dividing the data file into two data sets: data from the past 5 years was used to construct the models, and data from January to June of the 6th year was used to validate them. Simulation and forecasting performance was evaluated and compared between the two models. The hybrid SARIMA-GRNN model was found to outperform the SARIMA model with the lower mean square error, mean absolute error, and mean absolute percentage error in simulation and prediction results. Developing and applying the SARIMA-GRNN hybrid model is an effective decision supportive method for producing reliable forecasts of bacillary dysentery for the study area.
Japanese journal of infectious diseases. 07/2010; 63(4):264-70.
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ABSTRACT: Titanium dioxide (TiO(2)) is one kind of widely used nanoparticle, which was used as a solid-phase extraction to preconcentrated and measured of lead (Pb) in river water and seawater. However the interaction of nanoparticle TiO(2) and Pb was unknown. The aim of this study is to investigate the potential acute toxicity of the interaction between nanoparticle TiO(2) (50 and 120nm) and lead acetate (PbAC) in adult mice. The animals were randomly divided into six groups: a control group and five treatment groups (TiO(2)-50, TiO(2)-120, PbAC, TiO(2)-50+PbAC and TiO(2)-120+PbAC groups). Suspensions of TiO(2) (5g/kg body weight), PbAC (500mg/kg body weigh) and TiO(2) (5g/kg body weight)+PbAC (500mg/kg body weigh) were administrated to mice via oral gavage, respectively. Seven days later, the animals were sacrificed after being anesthetized by ether. There were no significant changes of the body weight coefficients of liver, kidney and brain. However, the results of liver function and nephrotoxicity examination revealed that there were serious damages to liver and kidney between the group treated with the mix suspension and the one with TiO(2). After the mix suspension treatment, ROS levels were significantly increased in liver but not in kidney, cortex and hippocampus. There were no increase of MDA levels in these tissues, and no activity reductions of SOD and GSH-Px in liver and kidney but in the cortex and hippocampus. Therefore, though our results have not suggested that TiO(2) particle and PbAC have a synergistic acute toxicity in mice after oral administration, PbAC may increase the acute toxicity of TiO(2) nanoparticle in some degree. The potential toxic mechanism maybe related with oxidative damages.
Environmental toxicology and pharmacology. 07/2010; 30(1):52-60.
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ABSTRACT: We developed in the present study a set of molecularly imprinted polymers (MIPs) by precipitation polymerization, using four members of tetracycline antibiotics (TCs) as templates (Oxytetracycline, Tetracycline, Chlortetracycline and Doxycycline). Then, based on the imprinting effect of different MIPs (TCs), Oxytetracycline and Chlortetracycline were chosen as mixed-templates to synthesize high selective MIPs for recognition of a family of analytes. And the characterization of obtained MIPs was analyzed by FESEM analysis, Brunauer-Emmett-Teller (BET) method, polymer size analysis, and determination of swelling ratio and COOH capacity. It was shown that the optimal imprinting factors of TCs on mixed-templates MIPs were more than 6.0 and the maximum binding amount of TCs were about 27 micromol g(-1) (Oxytetracycline), 35 micromol g(-1) (Tetracycline), 35 micromol g(-1) (Chlortetracycline) and 39 micromol g(-1) (Doxycycline), respectively. Finally, the mixed-templates MIPs were shown to be promising for on-line solid-phase extraction-HPLC-UV determination of trace TCs in foodstuffs. With a sample loading volume of 10 mL, the enhancement factors were in the range of 159-410.
Biosensors & bioelectronics 03/2010; 25(10):2218-24. · 5.43 Impact Factor
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ABSTRACT: About 40,000 inhabitants migrated from a high-risk area of esophageal squamous cell carcinoma (ESCC) to a low-risk area of esophageal cancer 40 years ago. Little is known about the change in the mortality in esophageal cancer among these immigrants. This study examined the impact of changing environments on esophageal cancer by comparing age-standardized mortality rates of immigrant group to the rates of native population (natives who live in high cancer location and have never moved) and host populations (hosts who live in low cancer location and have never moved people). All ESCC deaths taking place during 1999-2004 among the migrant, native, and host populations were identified by retrospective population-based screening. Direct age-adjusted mortality rates were calculated by using the China population of year 2000 as standard population. From 1999-2004, the average annual age-adjusted mortality of ESCC for the migrant, native, and host population was 61.6/100,000, 59.7/100,000, and 6.7/100,000, respectively. No decreasing tendency was found in mortality rate of ESCC in the population of young immigrants. The mortality rate of ESCC of migrants remained high even they had been living in the low endemic region for 40 years. This study strongly suggested that genetic susceptibility, rather than environment exposure, is responsible for the high risk of ESCC in the migrants.
Diseases of the Esophagus 11/2009; 23(5):392-7. · 1.81 Impact Factor
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ABSTRACT: Abstract: The molecularly imprinted polymer (MIP) was prepared by precipitation polymerization using Sudan Red I as the template. To investigate the influence of porogenic solvent and the amount of template on recognition property, selective chromatographic evaluation and frontal chromatography were performed. The results indicated that the obtained MIP had the best affinity and selectivity to the template when the molar ratio of template to functional monomer was 1:8 and a mixture of 30 mL methanol and 10 mL acetonitrile was used as the porogenic solvent. The imprinted factor of optimal MIP for Sudan Red I was 2.32, and the total amount of the immobilized ligand was 0.50 micromol/g. This MIP displayed good specific recognition property for Sudan Red I and was used as the sorbent of solid phase extraction (SPE) to determine trace Sudan Red I in chili powders. The linear range was from 10 to 500 micromol/L with a correlation coefficient of 0.999. The detection limit was 3.3 micromol/L, the spiked recovery was between 95.87% and 98.41%, and the relative standard deviation (RSD) was lower than 3.1%. The developed method can be used for the routine detection of Sudan Red I in chili powders.
Se pu = Chinese journal of chromatography / Zhongguo hua xue hui 11/2009; 27(6):764-8.
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ABSTRACT: Cisplatin is one of the most potent chemotherapeutic anticancer drugs for the treatment of various cancers. The cytotoxic action of the drug is often thought to be associated with its ability to bind DNA to form cisplatin-DNA adducts. Impaired DNA repair processes including base excision repair (BER) play important roles on its cytotoxicity. XRCC1 is a key protein known to play a central role at an early stage in the BER pathway. However, whether XRCC1 contributes to decrease the cisplatin cytotoxicity and cisplatin-induced DNA damage in HepG2 still remains unknown. Hence, the purpose of this study was to explore whether abrogation of XRCC1 gene expression by short hairpin RNAs (shRNA) could reduce DNA repair and thus sensitize liver cancer cells to cisplatin. We abrogated the XRCC1 gene in HepG2 cell using shRNA transfection. Cell viability was measured by MTT assay and clonogenicity assay. Comet assay was used to detect the DNA damage induced by cisplatin. The host cell reactivation was employed to assess the DNA repair capacity of cisplatin-damaged luciferase reporter plasmid. Flow cytometry analysis was used to determine cisplatin-induced apoptosis, cell cycle and reactive oxygen species (ROS). The results showed that abrogation of XRCC1 could sensitize HepG2 cells to cisplatin. This enhanced cytotoxicity could be attributed to the increased DNA damage and reduced DNA repair capacity. Increasing cell cycle arrest and intracellular ROS production lead to more tumor cell apoptosis and then enhanced the cisplatin cytotoxicity. Our results suggested that the cisplatin cytotoxicity may increase by targeting inhibition of XRCC1.
Toxicology Letters 10/2009; 192(2):108-14. · 3.23 Impact Factor
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ABSTRACT: The extreme vulnerability of developing nervous system to methylmercury (MeHg) is well documented. Still unclear is the consequence of different postnatal period exposure to MeHg. We investigated the critical postnatal phase when MeHg induced neurotoxicity in rats and the underlying mechanism. Rats were given 5mg/(kg day) methylmercury chloride (MMC) orally on postnatal day (PND) 7, PND14, PND28, and PND60 for consecutive 7 days. A control group was treated with 0.9% sodium chloride solution 5 ml/(kg day) instead. On PND69, spatial learning and memory was evaluated by Morris water maze test. Behavior deficits were found in MMC-treated rats of PND7 and PND14 groups (p<0.01). N-methyl-D-aspartate (NMDA) receptor 2 subunits mRNA expressions were evaluated 3 days after the last administration. In hippocampus, the mRNA expression of NR2A and NR2B decreased, but the NR2C expression increased in PND14 group following MMC-treatment (p<0.01). In cerebral cortex, mRNA expression of NR2A decreased, with NR2C expression elevating in PND14 group following MMC-treatment (p<0.05). These observations suggest that the postnatal exposure to MeHg during PND7-20 could cause neurobehavioral deficits which extend to adulthood. Furthermore, the abnormal expression of NMDAR 2 subunits might associate with the impairment.
Toxicology Letters 05/2009; 188(3):230-5. · 3.23 Impact Factor
