Maciej Kajor

Publications of Maciej Kajor

• Apoptosis of tubular epithelial cells in preimplantation biopsies of kidney grafts with immediate, slow and delayed function.

  Authors: Robert Krol, Jerzy Chudek, Henryk Karkoszka, Jacek Ziaja, Aurel Kolonko, Jacek Pawlicki, Maciej Kajor, Andrzej Wiecek, Lech Cierpka

  Annals of transplantation : quarterly of the Polish Transplantation Society. 09/2011; 16(3):17-22.

  Apoptosis is a form of cell death observed in kidney grafts as a result of ischemia/reperfusion injury. The aim of our prospective study was to analyze the intensity of apoptosis in kidney tubulesApoptosis is a form of cell death observed in kidney grafts as a result of ischemia/reperfusion injury. The aim of our prospective study was to analyze the intensity of apoptosis in kidney tubules after cold storage in respect to early and 12-month post-transplant graft function. The intensity of renal tubular apoptosis was estimated by TUNEL method in proximal and distal tubules in 72 pre-implantation kidney biopsies. Sixteen patients with biopsies that did not fulfill Banff 97 classification, with early acute rejection or early graft loss, were excluded. Early graft function was defined as IGF (N=17) when serum creatinine (sCr) was <264 µmol/l at 3(rd) postoperative day (POD); as SGF (N=20) when sCr >264 µmol/l and not more than 1 dialysis was performed; and as DGF (N=19) when more than 1 dialysis was done. The percentage of apoptotic cells was markedly higher in distal than in proximal tubules in all 3 groups. The percentage of apoptotic cells in distal tubules found was: 3.02% (1.03-5.00%) in IGF, 1.66% (0.92-2.39%) in SGF, and 1.76% (0.84-2.68%) in DGF; these differences were not significant. In the IGF group the mean percentage of apoptotic cells in distal tubule was higher than in the other groups (not statistically significant). The subgroups of patients with higher and lower than median (1.35%) apoptotic cell range in distal tubules had similar graft function at the 12-month follow-up. The enhancement of tubular epithelial cells apoptosis in kidney grafts after cold storage does not determine its early and later excretory function.

• Magnetic Resonance Tractography in Neuroradiological Diagnostic Aspects

  Authors: Jaroslaw Paluch, Jaroslaw Markowski, Tatiana Gierek, Przemyslaw Pencak, Malgorzata Witkowska, Maciej Kajor, Agnieszka Gorzkowska, Agnieszka Piotrowska

  09/2011;

  ISBN: 978-953-307-589-1

• A rare cause of chylous ascites.

  Authors: Krzysztof Gutkowski, Marek Hartleb, Andrzej Nowak, Maciej Kajor

  Liver international : official journal of the International Association for the Study of the Liver. 03/2011; 31(3):329.

• Expression, localization and systemic concentration of vascular endothelial growth factor (VEGF) and its receptors in patients with ulcerative colitis.

  Authors: Dorota Frysz-Naglak, Bogusława Fryc, Ewa Klimacka-Nawrot, Urszula Mazurek, Wanda Suchecka, Maciej Kajor, Józef Kurek, Antoni Stadnicki

  International immunopharmacology. 02/2011; 11(2):220-5.

  Vascular endothelial grow factor (VEGF) promotes angiogenesis by activating the specific receptors KDR and Flt-1. We investigate the expression of genes encoding VEGF and its receptors KDR and Flt- 1Vascular endothelial grow factor (VEGF) promotes angiogenesis by activating the specific receptors KDR and Flt-1. We investigate the expression of genes encoding VEGF and its receptors KDR and Flt- 1 by RT-QPCR reaction using Quanti Tect SYBR Green RT-PCR in patients with active and inactive ulcerative colitis (UC) and control subjects. The localization and level of VEGF protein and its receptors protein in intestinal tissue were estimated by immunohistochemistry. VEGF concentration in serum and plasma was determined by ELISA. We found a significant increase of VEGF gene expression and increase expression of genes encoding receptor Flt-1 in patients with active UC when compared with controls, but KDR was present in trace amount. VEGF and Flt-1 proteins were colocalized in enterocytes as well as in endothelium and muscularis layer of the intestine. The specific staining reaction for VEGF protein as well as for Flt-1 protein was significantly higher in active UC compared with controls. Serum level of VEGF was significantly higher in active UC patients as compared with inactive UC patients as well as with controls. The plasma VEGF level was found to be significantly higher in active UC patients as compared with controls. The increase of gene expression as well as protein level for VEGF and its receptor in UC - inflamed colon, and VEGF action via Flt-1 receptor may have a functional role in UC. Increased VEGF levels in both serum and plasma in active UC patients may reflect VEGF overexpression in intestinal inflammatory tissue.

• Surveyor nuclease detection of mutations and polymorphisms of mtDNA in children.

  Authors: Jacek Pilch, Marek Asman, Ewa Jamroz, Maciej Kajor, Elżbieta Kotrys-Puchalska, Małgorzata Goss, Maria Krzak, Joanna Witecka, Jan Gmiński, Aleksander L Sieroń

  Pediatric neurology. 11/2010; 43(5):325-30.

  Mitochondrial encephalomyopathies are complex disorders with wide range of clinical manifestations. Particularly time-consuming is the identification of mutations in mitochondrial DNA. A group of 20Mitochondrial encephalomyopathies are complex disorders with wide range of clinical manifestations. Particularly time-consuming is the identification of mutations in mitochondrial DNA. A group of 20 children with clinical manifestations of mitochondrial encephalomyopathies was selected for molecular studies. The aims were (a) to identify mutations in mtDNA isolated from muscle and (b) to verify detected mutations in DNA isolated from blood, in order to assess the utility of a Surveyor nuclease assay kit for patient screening. The most common changes found were polymorphisms, including a few missense mutations altering the amino acid sequence of mitochondrial proteins. In two boys with MELAS (i.e., mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke-like episodes), a mutation A→G3243 was detected in the tRNALeu gene of mtDNA isolated from muscle and blood. In one boy, the carrier status of his mother was confirmed, based on molecular analysis of DNA isolated from blood. A method using Surveyor nuclease allows systematic screening for small mutations in mtDNA, using as its source blood of the patients and asymptomatic carriers. The method still requires confirmation studying a larger group. In some patients, the use of this method should precede and might limit indications for traumatic muscle and skin biopsy.

• Liver visfatin expression in morbidly obese patients with nonalcoholic fatty liver disease undergoing bariatric surgery.

  Authors: Michał Kukla, Monika Ciupińska-Kajor, Maciej Kajor, Mariusz Wyleżoł, Krystyna Żwirska-Korczala, Marek Hartleb, Agnieszka Berdowska, Włodzimierz Mazur

  Polish journal of pathology : official journal of the Polish Society of Pathologists. 01/2010; 61(3):147-53.

  Visfatin has been identified as a new adipokine with proinflammatory and immunomodulating properties. It seems to interfere with immune and fibrogenic process in nonalcoholic fatty liver diseaseVisfatin has been identified as a new adipokine with proinflammatory and immunomodulating properties. It seems to interfere with immune and fibrogenic process in nonalcoholic fatty liver disease (NAFLD). The aim was to assess visfatin expression in the liver tissue and its association with biochemical parameters and morphological features in NAFLD patients. The study included 40 severely obese patients with NAFLD who underwent intraoperative wedge liver biopsy during a bariatric operation. Immunohistochemical assay was carried out with the use of a visfatin mice monoclonal antibody. Visfatin expression in the liver was observed in all patients. The expression was significantly higher in patients with fibrosis (p = 0.036) and was positively correlated with the fibrosis stage (r = 0.52, p = 0.03). There was no difference between patient with nonalcoholic steatohepatitis (NASH) and simple steatosis (p = 0.54). Inflammatory activity and NAS (NAFLD Activity Score) score were not associated with visfatin expression. There was a tendency of more evident visfatin liver expression in morbidly obese patients with diabetes mellitus. Our study showed a positive association between visfatin and the fibrosis stage in NAFLD. This observation suggests a potential role of this adipokine in the pathogenesis and progression of NAFLD. Visfatin expression does not seem to be associated with liver steatosis and inflammation.

• Validation of the BARD scoring system in Polish patients with nonalcoholic fatty liver disease (NAFLD).

  Authors: Joanna Raszeja-Wyszomirska, Barbara Szymanik, Małgorzata Ławniczak, Maciej Kajor, Alina Chwist, Piotr Milkiewicz, Marek Hartleb

  BMC gastroenterology. 01/2010; 10:67.

  Nonalcoholic fatty liver disease (NAFLD) includes a wide spectrum of liver diseases, ranging from pure steatosis to nonalcoholic steatohepatitis (NASH), and eventually to liver cirrhosis with itsNonalcoholic fatty liver disease (NAFLD) includes a wide spectrum of liver diseases, ranging from pure steatosis to nonalcoholic steatohepatitis (NASH), and eventually to liver cirrhosis with its complications. Identifying advanced fibrosis in patients is crucial to evaluating prognosis and possible therapeutic intervention. A novel, simple, and highly accurate scoring system called BARD, which identifies patients with NAFLD and without significant fibrosis, has been recently introduced and validated in North America..The aim of this study is to validate the BARD scoring system in a Polish cohort with NAFLD. A group of 104 Caucasians with biopsy-proven NAFLD were included in this study. Fibrosis in liver biopsies was evaluated according to the Histological Scoring System for Nonalcoholic Fatty Liver Disease. The BARD scoring system was assessed according to Harrison et al.: BMI > or = 28 = 1 point, AST/ALT ratio (AAR) > or = 0.8 = 2 points, type 2 diabetes mellitus = 1 point. Age over 50 and AAR over 0.8 showed, respectively, a moderate and strong association with advanced fibrosis. A BARD score of 2-4 points was associated with F3 or F4 stages of fibrosis with an odds ratio of 17.333 (95% Cl; 3,639 - 82.558) and negative predictive value of 97%. Our results demonstrate that the BARD scoring system has value in the non-invasive diagnosis of advanced fibrosis in NAFLD patients. The vast majority of patients with NAFLD would avoid liver biopsy if BARD was broadly introduced into the clinic.

• Platelet and intestinal 5-HT2A receptor mRNA in autistic spectrum disorders - results of a pilot study.

  Authors: Beata Kazek, Małgorzata Huzarska, Urszula Grzybowska-Chlebowczyk, Maciej Kajor, Monika Ciupińska-Kajor, Halina Woś, Elzbieta Marszał

  Acta neurobiologiae experimentalis. 01/2010; 70(2):232-8.

  The etiology and pathogenesis of autistic spectrum disorders (ASD) are still unknown. Platelet hyperserotonemia has been detected in 25-60% of autistic children. Higher incidence of gastrointestinalThe etiology and pathogenesis of autistic spectrum disorders (ASD) are still unknown. Platelet hyperserotonemia has been detected in 25-60% of autistic children. Higher incidence of gastrointestinal problems in people with autism is observed. The aim was compare the expression of platelet 5-HT(2A)r mRNA in autistic and non autistic groups. In a subgroup of patients with gastrointestinal problems an upper gastrointestinal tract endoscopy was performed and additionally the expression of 5-HT(2A) receptor mRNA in the duodenum was assessed. The examination was conducted in 79 children - 51 with ASD and 28 without autistic traits. Statistically significant differences between the study and control groups were proven in gastrointestinal problems. The analyses reveal a significantly higher level of 5-HT(2A)r mRNA in platelets of the study group patients, which could suggest serotonin system dysregulation.

• Serum chemerin and vaspin in non-alcoholic fatty liver disease.

  Authors: Michal Kukla, Krystyna Zwirska-Korczala, Marek Hartleb, Marek Waluga, Alina Chwist, Maciej Kajor, Monika Ciupinska-Kajor, Agnieszka Berdowska, Elzbieta Wozniak-Grygiel, Rafal Buldak

  Scandinavian journal of gastroenterology. 01/2010; 45(2):235-42.

  Chemerin and vaspin are new adipokines which may modulate inflammatory response and insulin sensitivity in non-alcoholic fatty liver disease (NAFLD). The aims of this study were to assess: (1)Chemerin and vaspin are new adipokines which may modulate inflammatory response and insulin sensitivity in non-alcoholic fatty liver disease (NAFLD). The aims of this study were to assess: (1) circulating levels of chemerin and vaspin and their association with liver histology and markers of liver injury in NAFLD patients; and (2) the relationship between the analyzed adipokines and insulin resistance. A total of 41 NAFLD patients with body mass index (BMI) 30.4 +/- 3.3 kg/m(2) [20 with non-alcoholic steatohepatitis (NASH) and BMI 30.3 +/- 3.3 kg/m(2) and 21 with simple steatosis/uncertain NASH (SS/UN) and BMI 30.5 +/- 3.4 kg/m(2)] and 10 healthy volunteers with BMI 24.0 +/- 2.9 kg/m(2) were included in the study. Serum chemerin concentration was significantly higher in NAFLD patients compared to healthy volunteers (p = 0.009). Serum chemerin was significantly higher in patients with NASH compared to patients with SS/UN (p = 0.009). The homeostasis model assessment for insulin resistance (HOMA-IR) value was higher in patients with NASH than in patients with SS/UN (p = 0.01). Serum chemerin and HOMA-IR were positively associated with NAFLD activity score (r = 0.40, p = 0.02; and r = 0.43, p = 0.008, respectively). Serum chemerin was associated with hepatocyte ballooning degeneration (r = 0.37; p = 0.03), total cholesterol (r = 0.45; p = 0.008) and diastolic blood pressure (r = 0.41; p = 0.02). HOMA-IR was related to fibrosis stage (r = 0.51; p = 0.001) and inflammatory activity grade in portal tracts (r = 0.40; p = 0.01). Serum vaspin correlated with hepatocyte ballooning degeneration (r = 0.31; p = 0.04), alanine aminotransferase and aspartate aminotransferase (r = 0.33, p = 0.03; and r = 0.32, p = 0.04, respectively) and diastolic blood pressure (r = 0.39, p = 0.01). This study shows for the first time that chemerin and vaspin serum concentrations are altered in patients with NAFLD. The analyzed adipokines appear to play a pivotal role in the pathogenesis of NAFLD, not only as regulators of insulin sensitivity, but also as mediators of the inflammatory process.

• Diazoxide protects myocardial mitochondria, metabolism, and function during cardiac surgery: a double-blind randomized feasibility study of diazoxide-supplemented cardioplegia.

  Authors: Marek A Deja, Marcin Malinowski, Krzysztof S Gołba, Maciej Kajor, Tomasz Lebda-Wyborny, Damian Hudziak, Wojciech Domaradzki, Dariusz Szurlej, Andrzej Bończyk, Jolanta Biernat, Stanisław Woś

  The Journal of thoracic and cardiovascular surgery. 05/2009; 137(4):997-1004, 1004e1-2.

  OBJECTIVES: The study was designed to assess whether diazoxide-mediated cardioprotection might be used in human subjects during cardiac surgery. METHODS: Forty patients undergoing coronary arteryOBJECTIVES: The study was designed to assess whether diazoxide-mediated cardioprotection might be used in human subjects during cardiac surgery. METHODS: Forty patients undergoing coronary artery bypass grafting were randomized to receive intermittent warm blood antegrade cardioplegia supplemented with either diazoxide (100 micromol/L) or placebo (n = 20 in each group). Mitochondria were assessed before and after ischemia and reperfusion in myocardial biopsy specimens. Myocardial oxygen and glucose and lactic acid extraction ratios were measured before ischemia and in the first 20 minutes of reperfusion. Hemodynamic data were collected, and troponin I, creatine kinase-MB, and N-terminal prohormone brain natriuretic peptide levels were measured. All outcomes were analyzed by using mixed-effects modeling for repeated measures. RESULTS: No deaths, strokes, or infarcts were observed. Patients received, on average, 36.2 +/- 1.2 mg of diazoxide and 37.3 +/- 1.9 mg of placebo (P = .6). Diazoxide added to cardioplegia prevented mitochondrial swelling (8899 +/- 474 vs 9273 +/- 688 pixels before and after the procedure, respectively; P = .6) compared with that seen in the placebo group (8474 +/- 163 vs 11,357 +/- 759 pixels, P = .004). No oxygen debt was observed in the diazoxide group. Glucose consumption and lactic acid production returned to preischemic values faster in the diazoxide group. The following hemodynamic parameters differed between the diazoxide and placebo groups, respectively, in the postoperative period: cardiac index, 3.0 +/- 0.09 versus 2.6 +/- 0.09 L . min(-1) . m(-2) (P = .002); left cardiac work index, 2.81 +/- 0.07 versus 2.31 +/- 0.07 kg/m(2) (P < .001); oxygen delivery index, 420 +/- 14 versus 377 +/- 13 mL . min(-1) . m(-2) (P = .03); and oxygen extraction ratio, 29.3% +/- 1.1% versus 32.6% +/- 1.1% (P = .02). Postoperative myocardial enzyme levels did not differ, but N-terminal prohormone brain natriuretic peptide levels were lower in the diazoxide group (120 +/- 27 vs 192 +/- 29 pg/mL, P = .04). CONCLUSIONS: Supplementing blood cardioplegia with diazoxide is safe and improves myocardial protection during cardiac surgery, possibly through its influence on the mitochondria.

• [Changes of mRNAs encoding alternatively spliced variants of procollagen C-endopeptidase in leiomyomas uteri depending on the phase of menstrual cycle and in postmenopausal women]

  Authors: Aleksandra Auguściak-Duma, Maciej Kajor, Magdalena Piwowarczyk, Jerzy Sikora, Aleksander L Sieron

  Ginekologia polska. 02/2009; 80(1):25-32.

  Leiomyoma uteri is a monoclonal tumour of the uterus muscle layer. It is characterized by excessive, abnormal growth of extracellular matrix. The collagen types I and III are the major components ofLeiomyoma uteri is a monoclonal tumour of the uterus muscle layer. It is characterized by excessive, abnormal growth of extracellular matrix. The collagen types I and III are the major components of extracellular matrix. Removal of the C-propeptides in procollagens type I, II, and III by procollagen C-endopeptidase leads to spontaneous self-assembly of collagen fibrils. Thus, the procollagen C-endopeptidase is a key regulator of extracellular matrix production, its quality, and other developmental processes including angiogenesis. OBJECTIVE: The objective of this study was to analyze the three alternatively spliced variants of the BMP1 gene product including the procollagen C-endopeptidase, in leiomyoma uteri tumors in comparison to normal myometrium in women being in first or second menstrual phase or in time of climacterium. MATERIAL AND METHODS: In the study we analyzed samples from the control and cancerous tissues from 52 women. Expression of the three alternatively spliced variants of BMP1 gene transcript were assayed by RT-PCR, following densytometric analysis. Statistical significance (p < or = 0,05) of the observed differences was assessed with the use of Anova test, Least Significance Different Test, and Student's T-test. RESULTS: Analysis of RT-PCR products revealed the presence of all alternatively spliced variants of BMP-1 mRNA and the changes in their alternative splicing intensity, depending on the phase of menstrual cycle or postmenopausal state. In the second phase of the cycle, in control tissue, the expression level of BMP-1 variant decreased when compared to women in I phase of the cycle or postmenopausal women. In the tumour, postmenopausal women showed increased expression of BMP-1at, when compared with women in the second phase of the cycle. The presence of mTLD in the tumour tissue at II phase of the cycle and in postmenopausal state was less strong when compared to women at I phase of the cycle. In control tissue this type of change was not observed. The BMP-1/HIS was present at higher level in control tissue, during II phase of the menstrual cycle and in postmenopausal state, whereas in the tumour tissue its lowest level was at II phase of the cycle. CONCLUSIONS: Regulation of alternative splicing of mRNA for procollagen C-endopeptidase in leiomyomas and myometrium depends mainly on the hormonal status of women.

• NBL1 and anillin (ANLN) genes over-expression in pancreatic carcinoma.

  Authors: Marek Olakowski, Tomasz Tyszkiewicz, Michał Jarzab, Robert Król, Małgorzata Oczko-Wojciechowska, Małgorzata Kowalska, Monika Kowal, Grzegorz M Gala, Maciej Kajor, Dariusz Lange, Ewa Chmielik, Elzbieta Gubala, Paweł Lampe, Barbara Jarzab

  Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society. 01/2009; 47(2):249-55.

  The aim of the study was to analyze the gene expression profile of pancreatic cancer to derive novel molecular markers of this malignancy. The snap-frozen or RNA-later preserved samples of 18The aim of the study was to analyze the gene expression profile of pancreatic cancer to derive novel molecular markers of this malignancy. The snap-frozen or RNA-later preserved samples of 18 pancreatic adenocarcinomas, 5 chronic pancreatitis cases and 6 specimens of grossly normal pancreas were used for microarray analysis by HG-U133 Plus 2.0 oligonucleotide Affymetrix arrays. Validation was carried out by real-time quantitative PCR (Q-PCR) in the set of 66 samples: 31 of pancreatic cancer, 14 of chronic pancreatitis and 21 of macroscopically unchanged pancreas. By Principal Component Analysis of the microarray data we found a very consistent expression pattern of normal samples and a less homogenous one in chronic pancreatitis. By supervised comparison (corrected p-value 0.001) we observed 11094 probesets differentiating between cancer and normal samples, while only seventy six probesets were significant for difference between cancer and chronic pancreatitis. The only gene occurring within the best 10 genes in both comparisons was S100 calcium binding protein P (S100P), already indicated for its utility as pancreatic cancer marker by earlier microarray-based studies. For validation we selected two genes which appeared as valuable candidates for molecular markers of pancreatic cancer: neuroblastoma, suppression of tumorigenicity 1 (NBL1) and anillin (ANLN). By Q-PCR, we confirmed statistically significant differences in these genes with a 9.5 fold-change difference between NBL1 expression in cancer/normal comparison and a relatively modest difference between cancer and pancreatitis. For ANLN even more distinct differences were observed (cancer/normal 19.8-fold, cancer/pancreatitis 4.0-fold). NBL1 and anillin are promising markers for pancreatic carcinoma molecular diagnostics.

• [Case of malignant tumour phyllodes converting to fibrosarcoma]

  Authors: Jacek Pawlicki, Robert Król, Maciej Kajor, Jacek Ziaja

  Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego. 04/2007; 22(129):215-7.

  Tumour phyllodes is rare breast neoplasm. The majority arises in women between ages 35 and 55. Tumours phyllodes are composed of hypercellular mesenchymal stroma and epithelial elements. They areTumour phyllodes is rare breast neoplasm. The majority arises in women between ages 35 and 55. Tumours phyllodes are composed of hypercellular mesenchymal stroma and epithelial elements. They are commonly classified as benign, rarely as borderline or malignant. Recurrences are observed in 8-36% patients and metastases--in 13-26%. There is a case of large (28 x 24 cm) malignant tumour phyllodes presented in the article. Mastectomy was performed. After surgical treatment of recurrent tumours (fibrosarcoma form) occurred two times during 1 year time. They were operated immediately. In spite of unfavorable prognostic features of the tumour (large size, malignant histological character) and recurrences, final therapeutic effect was good.

• Lipid raft disease? A new severe congenital myopathy.

  Authors: Maciej Kajor, Katarzyna Wojaczyńska-Stanek, Elzbieta Marszał, Urszula Grzybowska-Chlebowczyk, Anna Fidziańska

  Folia neuropathologica / Association of Polish Neuropathologists and Medical Research Centre, Polish Academy of Sciences. 02/2007; 45(4):242-6.

  We report a 5-year-old girl with a unique neuromuscular disorder manifested by early onset of the disease, delayed motor development, joint contractures, dysmorphy, cobbler's chest, generalizedWe report a 5-year-old girl with a unique neuromuscular disorder manifested by early onset of the disease, delayed motor development, joint contractures, dysmorphy, cobbler's chest, generalized muscle hypoplasia and weakness. Morphological examination revealed muscle cell immaturity and the appearance of multilamellar myelin-like structures within and outside the sarcolemma. Overexpression of aberrant lipids on the surface of affected muscle cells may suggest some failure in lipid raft formation.

• [Malignant lymphoma or inflammation? Diagnostic and treatment difficulties--2 cases report]

  Authors: Bogusława Orzechowska-Wylegała, Maciej Kajor, Marcin Mazur, Anatol Wodołazski, Piotr Malara, Jan Drugacz

  Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego. 05/2006; 20(118):433-6.

  Soft and hard tissue in the oral cavity can be the first coexisting place of systemic diseases manifestation. The aim of this study was to report the diagnostic and treatment difficulties in twoSoft and hard tissue in the oral cavity can be the first coexisting place of systemic diseases manifestation. The aim of this study was to report the diagnostic and treatment difficulties in two patients with malignant lymphomas. The first case was a 66-year-old woman who had undergone surgery procedures connected with radiotherapy and chemotherapy because of malignant lymphoma in nasal cavity in 2001 year. In 2002 she was treated in our department after finding tumor in oral cavity, also diagnosed as malignant lymphoma. The second patient was a 58-year-old man who had undergone an operation (surgery) by reason of maxillary inflammation tumor in 2002 year. On the next year he underwent an operation of removal cervical lymp nodes because of malignant lymphoma. In 2004 year he underwent surgery on account of maxillary tumor suspected by malignant lymphoma, which was diagnosed as inflammation. Conclusions. In differentiation tumors of head and neck one should take an account of lymphoma malignant. The similar clinic and radiological symptoms and laboratory results of malignant lymphomas and inflammations of head and neck can cause the late diagnosis and aggravate the prognosis of this severe disease.

• Hereditary neuropathy with liability to pressure palsy.

  Authors: Justyna Paprocka, Maciej Kajor, Ewa Jamroz, Aleksandra Jezela-Stanek, Pavel Seeman, Elzbieta Marszał

  Folia neuropathologica / Association of Polish Neuropathologists and Medical Research Centre, Polish Academy of Sciences. 02/2006; 44(4):290-4.

  Hereditary neuropathy with liability to pressure palsy (HNPP) is an autosomal dominant disease with sensory and motor nerve palsies usually precipitated by trivial trauma or compression. In theHereditary neuropathy with liability to pressure palsy (HNPP) is an autosomal dominant disease with sensory and motor nerve palsies usually precipitated by trivial trauma or compression. In the majority of cases HNPP is caused by deletion of the peripheral myelin protein 22 gene (PMP22) on chromosome 17p11.2. The authors present a family case with genetically proven HNPP.

• [Churg-Strauss syndrome--a case report]

  Authors: Antoni Hrycek, Grazyna Pochopień-Kenig, Maria Wanat-Wiśniewska, Maciej Kajor

  Wiadomości lekarskie (Warsaw, Poland : 1960). 02/2006; 59(1-2):117-21.

  A case of a 58-year-old woman with Churg-Strauss syndrome is reported. After therapy with steroids clinical improvements were obtained. The discussion concerning the pathogenesis of disease wasA case of a 58-year-old woman with Churg-Strauss syndrome is reported. After therapy with steroids clinical improvements were obtained. The discussion concerning the pathogenesis of disease was performed.

• Skeletonization of internal thoracic artery affects its innervation and reactivity.

  Authors: Marek Andrzej Deja, Krzysztof S Gołba, Marcin Malinowski, Stanisław Woś, Maciej Kolowca, Jolanta Biernat, Maciej Kajor, Tomasz J Spyt

  European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery. 10/2005; 28(4):551-7.

  OBJECTIVE: The studies showing the superior characteristics of ITA graft and its impact on the clinical results of coronary artery surgery were performed with ITA harvested almost exclusively as aOBJECTIVE: The studies showing the superior characteristics of ITA graft and its impact on the clinical results of coronary artery surgery were performed with ITA harvested almost exclusively as a pedicle. This study assesses the impact of ITA skeletonization on its innervation and reactivity. METHODS: Segments of skeletonized and non-skeletonized ITA were stained with antibodies against protein S-100 to look for the presence of sympathetic nerve fibers. The functional studies were performed on segments of discarded human pedicled ITA that were divided into two 3mm rings, one skeletonized and another non-skeletonized. We compared concentration-effect relationships for the contraction to norepinephrine and endothelium-dependent relaxation to acetylcholine and bradykinin, as well as endothelium-independent relaxation to sodium nitroprusside in skeletonized and non-skeletonized segments of the same ITA. RESULTS: Skeletonized ITA was devoid of protein S-100 positive nerve fibers. It contracted stronger (maximal response 37.0+/-2.04 vs. 25.4+/-1.83mN (P<0.001)) and was twice as sensitive to norepinephrine: pD(2) 6.03+/-0.10 vs. 5.70+/-0.12 (P=0.035). The endothelium-dependent relaxation responses did not differ between skeletonized and non-skeletonized ITA rings. The skeletonized ITA rings appeared over 10 times more sensitive to sodium nitroprusside: pD(2) 6.66+/-0.20 vs. 5.59+/-0.37 (P=0.012)-potency ratio 11.61. The maximal responses did not differ significantly: 112.0+/-6.71 vs. 129.4+/-16.4% (P=0.33). CONCLUSIONS: Skeletonization results in sympathectomy of ITA. It has no effect on endothelium-dependent relaxation but increases reactivity of ITA to norepinephrine. This augmented response to alpha-agonist is small, in comparison with over a ten-fold increase in sensitivity to sodium nitroprusside. Pedicled and skeletonized ITA are functionally significantly different vessels when studied in vitro.

• [Juvenile dermatomyositis in 12 years old girl]

  Authors: Beata Bergler-Czop, Ligia Brzezińska-Wcisło, Anna Lis, Ewa Krauze, Elzbieta Marszał, Ewa Jamroz, Beata Klimek, Maciej Kajor

  Wiadomości lekarskie (Warsaw, Poland : 1960). 02/2005; 58(9-10):567-71.

  We would like to present a selected case of 12-aged girl, with recognition of dermatomyositis (DM). At the age of 11 in the normally developing child, erythematous-oedematous changes have appeared onWe would like to present a selected case of 12-aged girl, with recognition of dermatomyositis (DM). At the age of 11 in the normally developing child, erythematous-oedematous changes have appeared on the face, particularly intensified in the vicinity of the orbital cavities (so called: pseudoglasses), as well as erythema and teleangiectasis on the dorsum of hands and small-sized diarthroidal joints (the Gottron's symptom). Subsequently, lower physical efficiency and distinctly weakness in the child's extremities occurred. In EMG (quadriceps muscle of the thigh) myogenous traits have been proven. Neurological examination revealed as follows: muscular weakness (adynamia), mainly lower limbs (grade 3 in the Lovett's scale, along with decreased loss of muscles tone), lack of the periosteal reflex near lower limbs, positive Gower's symptom and increased anterior spinal curvature. In the biochemical examinations accelerated erythrocyte sedimentation rate (ESR), and a rise of activity in muscles enzymes were stated. In the child's blood serum, we disclosed antinuclear antibodies ANA (type of granular luminescence, titre 160), to be rather evident to presence of autoimmunological process. During examinations of the musculocutaneus specimen, DM-markers have been detected. Capillaroscopy proved specific presence of numerous vessels, multiple capillary tubes, individual gemmated vessels and completely invisible dermatomyositous border. Patient was treated with per os sterid--Encorton at the initial dose of 2 mg/day, every other day during the lapse of 6 weeks to reach the normal CPK-activity, and consequently clinical picture under "on-line" surveillance, gradually reducing a specific medicine up to maintenance dose through 18 months. At present, the patient is subjected to check-up and monitoring by Neurological Outpatient and Rehabilitain Clinic for Children.

• [Clinical picture of celiac disease in children]

  Authors: Urszula Grzybowska-Chlebowczyk, Halina Woś, Sabina Wiecek, Maciej Kajor, Maria Szymańska, Anna Staszewska-Kwak, Marzena Piatkowska, Dariusz Gołka

  Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego. 02/2005; 18(103):49-53.

  During the last few years we have been observing the decreasing incidence of the symptomatic celiac disease and increasing incidence of mono- and asymptomatic disease. Various atypical symptoms andDuring the last few years we have been observing the decreasing incidence of the symptomatic celiac disease and increasing incidence of mono- and asymptomatic disease. Various atypical symptoms and extraintestinal manifestations were observed in older children (above 7 years) and in adults. Clinical symptoms of celiac disease may appear at any age, in each of the following forms: clinical celiac disease (symptomatic), silent (oligosymptomatic) or latent, with late manifestation, being the effect of stress, pregnancy, infections or excessive dietary gluten provocation. THE AIM OF THE STUDY: The presentation the variety of clinical pictures of celiac disease in children. MATERIALS AND METHODS: We have evaluated the clinical course of celiac disease in 16 children (13 girls and 3 boys), aged between 12 months to 17 years (mean-7,5 years). The celiac disease was diagnosed on the grounds of clinical symptoms, histopathological examination of the small intestinal endoscopic biopsy and immunological examinations: serum antitransglutaminase antibodies and anti-endomysium antibodies. RESULTS: Most commonly diagnosed form was the silent, monosymptomatic celiac disease. Four children manifested with growth retardation, one with osteoporosis, and one with iron deficiency anemia resistant to treatment. Two girls (aged 16 and 17 years) presented with symptomatic celiac disease. In 3 patients despite the gastrointestinal symptoms and histopathological changes present (villous atrophy, Marsh's index > 40) we did not find any immunological markers of celiac disease (with normal IgA levels). CONCLUSIONS: School-aged children with celiac disease, in many cases have no evident clinical and functional disorders caused by gluten intake, despite to presence of considerable abnormalities in the histopathological examination.