A Bottalico

Publications (54)49.05 Total impact

• Article: Toxicity of mycotoxins of Fusarium sambucinum for feeding in Galleria mellonella

  G. Mulè, A. D'Ambrosio, A. Logrieco, A. Bottalico
  [show abstract] [hide abstract]
  ABSTRACT: Fusarium sambucinum Fuckel showed antifeedant activity towards larvae of Galleria mellonella L. when incorporated into insect diet. The activity appeared mostly due to the concentration of trichothecenes present in the fungal extracts. Diacetoxyscirpenol and neosolaniol showed similar levels of activity and were significant potent antifeedants against larvae at 50 and 100 ppm. On the contrary, enniatin B showed no activity up to 100 ppm.
  Entomologia Experimentalis et Applicata 04/2011; 62(1):17 - 22. · 1.53 Impact Factor
• Article: Pathogenicity of Fusarium graminearum Chemotypes Towards Corn, Wheat, Triticale and Rye

  A. Logrieco, M. Manka, C. Altomare, A. Bottalico
  [show abstract] [hide abstract]
  ABSTRACT: The pathogenicity of nine Fusarium graminearum isolates belonging to three different trichothecene-producing chemotypes, i.e. NIV + FUS, DON + 3-AcDON, and DON + 15-AcDON was tested on corn, wheat, triticale and rye. Although these compounds are known to have different toxicity, no difference in pathogenicity was observed among the mean values for chemotypes. However, within each chemotype there were isolates with different pathogenicity which was not correlated with the ability to produce trichothecenes in vitro. The pathogenicity of each isolate was almost the same for all tested cereals. The involvement of trichothecenes in pathogenesis was investigated in infected corn stalk tissues. All the expected trichothecenes were detected with the exception of NIV and FUS.
  Journal of Phytopathology 06/2008; 130(3):197 - 204. · 0.79 Impact Factor
• Article: Occurrence and Pathogenicity of Fusarium Species in Banana Fruits

  M. Jiménez, A. Logrieco, A. Bottalico
  [show abstract] [hide abstract]
  ABSTRACT: Banana fruits were studied over a six-month period in order to determine the incidence of species of the Fusarium genus and assess their potential pathogenicity. The 72 samples studied were commercially available in Italy and Spain, where they were brought from Panama, Ecuador and Canary Islands. Among the species detected in the fruits, Fusarium semitectum var. majus Wollenw. was predominant, followed by F. moniliforme Sheld., F. solani (Mart.) Appel & WoUenw., F. oxysporum Schlecht., F. proliferatum (Matsushima) Nirenberg, F. graminearum Schw., F. camptoceras WoUenw. &C Reinking, F. subglutinans (WoUenw. & Reinking) Nelson et al., F. dimerum Penzig in Sacc, F. acuminatum EU. & Ev., and F. equiseti (Corda) Sacc. Fusarium proliferatum had never been reported to occur as contaminating fungi in banana fruits to date. Fusarium subglutinans, F. acuminatum and F. graminearum were found to be the most markedly pathogenic of all. The lack of noticeable differences in relation to the incidence of the different species isolated from the samples indicates that the mycoflora found is typical of this fruit and does not depend on its origin.
  Journal of Phytopathology 04/2008; 137(3):214 - 220. · 0.79 Impact Factor
• Article: Antagonistic Activity in Fusarium acuminatum

  A. Logrieco, G. Mule, A. Bottalico
  [show abstract] [hide abstract]
  ABSTRACT: Ten of 19 (53%) tested isolates of Fusarium acuminatum, from different geographical origins and sources, showed in vitro antagonistic activity (inhibition at distance) to mycelial growth of F. moniliforme. Moreover, when F. acuminatum ITEM-728 was tested against 25 different fungal species, an initial inhibition at a distance was observed which was followed by the spread of the F. acuminatum mycelium over the opposite fungal colony to various degrees. Most of the F. acuminatum isolates which showed antagonistic activity proved to be enniatin B (EB) producers, and some of them also formed moniliformin (MF). The toxic activity of the methanol extract of F. acuminatum ITEM-728 towards some test microorganisms was closely related to the EB concentration. In particular, Bacillus subtilis proved to be a very sensitive test microorganism.ZusammenfassungZehnvon 19(53 %) geprüften Fusarium acuminatum-Isolaten, dieausunterschiedlichenSubstraten und Standorten herstammten, zeigten in vitro antagonistische Aktivität (Bildung von Hemmhöfen) gegen das Myzelwachstum von F. moniliforme. Außerdem konnte mit dem F. acuminatum-Sxamra ITEM-728 in einer Prüfung gegen 25 Pilzarten gezeigt werden, daß, nach einer anfänglichen, als Hemmhofbildung gekennzeichneten Aktivität, das F. acuminatum-Myzti die andere Pilzkolonie zu unterschiedlichen Graden überwuchs. Der Hauptanteii der F. acumtnatum-Isolate mit antagonistischer Aktivität produzierte Enniatin B (EB). Einige dieser Isolate konnten auch Moniliformin (MF) produzieren. Die tojtische Aktivität des Methanolextraktes von F. acuminatum HEM-728 gegen einige Prüfmikroorganismer wurde hauptsächlich mit der EB-Konzentration verglichen. Bacillus subtilis zeigte sich als ein besonders empfindlicber Prüfmikroorgamsmus.
  Journal of Phytopathology 04/2008; 140(3):193 - 200. · 0.79 Impact Factor
• Article: Isolation of Cytochalasins A and B from Ascochyta heteromorpha

  R. Capasso, A. Evidente, G. Randazzo, A. Ritieni, A. Bottalico, M. Vurro, A. Logrieco
  06/2004;
• Article: Three New Toxic Pinolidoxins from Ascochyta pinodes

  A. Evidente, R. Capasso, M. A. Abouzeid, R. Lanzetta, M. Vurro, A. Bottalico
  06/2004;
• Article: Structure of Cavoxin, a New Phytotoxin from Phoma cava and Cavoxone, Its Related Chroman-4-One

  A. Evidente, G. Randazzo, N. S. Iacobellis, A. Bottalico
  06/2004;
• Article: Toxigenic Fusarium Species and Mycotoxins Associated with Maize Ear Rot in Europe

  A. Logrieco, G. Mulè, A. Moretti, A. Bottalico
  [show abstract] [hide abstract]
  ABSTRACT: Several Fusarium species occurring worldwide on maize as causal agents of ear rot, are capable of producing mycotoxins in infected kernels, some of which have a notable impact on human and animal health. The main groups of Fusarium toxins commonly found are: trichothecenes, zearalenones, fumonisins, and moniliformin. In addition, beauvericin and fusaproliferin have been found in Fusarium-infected maize ears. Zearalenone and deoxynivalenol are commonly found in maize red ear rot, which is essentially caused by species of the Discolour section, particularly F. graminearum. Moreover, nivalenol and fusarenone-X were often found associated with the occasional occurrence of F. cerealis, and diacetoxyscirpenol and T-2 toxin with the occurrence of F. poae and F. sporotrichioides, respectively. In addition, the occurrence of F. avenaceum and F. subglutinans usually led to the accumulation of moniliformin. In maize pink ear rot, which is mainly caused by F. verticillioides, there is increasing evidence of the wide occurrence of fumonisin B1. This carcinogenic toxin is usually found in association with moniliformin, beauvericin, and fusaproliferin, both in central Europe due to the co-occurrence of F. subglutinans, and in southern Europe where the spread of F. verticillioides is reinforced by the widespread presence of F. proliferatum capable of producing fumonisin B1, moniliformin, beauvericin, and fusaproliferin.
  European Journal of Plant Pathology 08/2002; 108(7):597-609. · 1.41 Impact Factor
• Source

  Available from: nih.gov

  Article: Clustering of trichothecene-producing Fusarium strains determined from 28S ribosomal DNA sequences.

  G Mulè, A Logrieco, G Stea, A Bottalico
  [show abstract] [hide abstract]
  ABSTRACT: The genus Fusarium includes several species that produce trichothecenes. We analyzed DNA sequences from a variable region at the 5' end of the large nuclear ribosomal DNA (rDNA) (28S) to determine the genetic relatedness of trichothecene-producing Fusarium species. All trichothecene-producing strains clustered together, and two monophyletic groups were resolved. The first clade included strains of F. acuminatum, F. sambucinum, F. tumidum, F. compactum, F. camptoceras (red pigment), F. sporotrichioides, and F. venenatum, which produced type A trichothecenes (T-2 toxin, HT-2 toxin, neosolaniol, and diacetoxyscirpenol). The second clade consisted of strains of F. crookwellense, F. culmorum, and F. graminearum, which produced type B trichothecenes (fusarenone-X, nivalenol, and deoxynivalenol). The phylogenetic placement of the species based on rDNA correlated better with toxic secondary metabolite data rather than with the current classification system based on morphology.
  Applied and Environmental Microbiology 06/1997; 63(5):1843-6. · 3.83 Impact Factor
• Article: Toxic metabolites from phytopathogenic Ascochyta species.

  A Evidente, R Capasso, A Motta, A Andolfi, M Vurro, M C Zonno, A Bottalico
  [show abstract] [hide abstract]
  ABSTRACT: We review some chemical and biological aspects of toxic metabolites produced in vitro by phytopathogenic Ascochyta species, fungi having agrarian and toxicological importance. In particular, here the isolation of some known and new cytochalasins from A. heteromorpha and A. lathyri, four new nonenolides from A. pinodes and a new trisubstituted derivative of salycilic aldehyde from A. pisi are described. Furthermore, the identification of medicarpin, phytoalexin found in the chickpea seeds naturally infected by A. rabiei is also reported.
  Bollettino chimico farmaceutico 11/1996; 135(9):552-5.
• Source

  Available from: Francesca Fornelli

  Article: Fusaproliferin production by Fusarium subglutinans and its toxicity to Artemia salina, SF-9 insect cells, and IARC/LCL 171 human B lymphocytes.

  A Logrieco, A Moretti, F Fornelli, V Fogliano, A Ritieni, M F Caiaffa, G Randazzo, A Bottalico, L Macchia
  [show abstract] [hide abstract]
  ABSTRACT: Fusarium subglutinans is an important pathogen of maize and other commodities worldwide. We examined MRC-115 and 71 other F. subglutinans strains from various geographic areas for their ability to synthesize fusaproliferin, a novel toxic sesterterpene recently isolated from F. proliferatum. Fusaproliferin production ranged from 30 to 1,500 micrograms/g of dried ground substrate, with 33 strains producing more than 500 micrograms/g. In particular, strain MRC-115 produced as much as 1,100 to 1,300 micrograms/g. In toxicity studies of two invertebrate models, fusaproliferin was toxic to Artemia salina (50% lethal dose, 53.4 microM) and to the lepidopteran cell line SF-9 (50% cytotoxic concentration, approximately 70 microM, after a 48-h exposure). Fusaproliferin was also toxic to the human nonneoplastic B-lymphocyte cell line IARC/LCL 171 (50% cytotoxic concentration, approximately 55 microM in culture in stationary phase after a 48-h exposure). Experiments performed will cells exposed at seeding suggested a possible cytostatic effect at subtoxic concentrations.
  Applied and Environmental Microbiology 10/1996; 62(9):3378-84. · 3.83 Impact Factor
• Article: Cytochalasin W, a new 24-oxa[14]cytochalasan from Phoma exigua var. heteromorpha.

  A Evidente, R Capasso, M Vurro, A Bottalico
  [show abstract] [hide abstract]
  ABSTRACT: A further cytochalasin was isolated from liquid culture filtrates of Phoma exigua var. heteromorpha together with cytochalasins U and V and other well-known cytochalasins. The metabolite, named cytochalasin W, was characterized by spectroscopic and chemical methods as a new 24-oxa[14]cytochalasan, bearing a formyl group on the macrocyclic ring. Cytochalasin W showed toxic activity in the brine shrimp assay.
  Natural Toxins 02/1996; 4(2):53-7.
• Article: Paracelsin E, a new peptaibol from Trichoderma saturnisporum.

  A Ritieni, V Fogliano, D Nanno, G Randazzo, C Altomare, G Perrone, A Bottalico, L Maddau, F Marras
  [show abstract] [hide abstract]
  ABSTRACT: The structure of paracelsin E, a new peptaibol from Trichoderma saturnisporum, has been determined primarily by fabms. The well-known paracelsins A, B, C, and D were also found in a culture of this organism.
  Journal of Natural Products 12/1995; 58(11):1745-8. · 3.13 Impact Factor
• Article: Fusarium species associated with banana fruit rot and their potential toxigenicity.

  R F Vesonder, A Logrieco, A Bottalico, C Altomare, S W Peterson
  [show abstract] [hide abstract]
  ABSTRACT: Banana fruits exhibiting signs of decay were collected from markets in the United States and Italy. Fungi isolated from the lesions on the banana fruits wereFusarium moniliforme, F subglutinans, andF. semitectum var.majus. When the fungal strains were cultivated on maize kernels, the cultures did not produce zearalenone (ZON), zearalenols (á-, â-ZOH), and trichothecenes [deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-AcDON), T-2 toxin (T-2), diacetoxyscirpenol (DAS)]. Fumonisins and fusarin C (FUS-C) were not detected naturally nor in bananas purchased in the U.S. and artificially infected withFusarium. Moniliformin (M) (up to 267 mg/kg) was detected in maize kernel cultures ofF. subglutinans from bananas. No mycotoxins were detected in naturally infected fruits. Although no mycotoxins were detected in the extracts from corn cultures ofF. semitectum var.majus, the extracts were toxic to brine shrimp and mice.
  Mycotoxin Research 09/1995; 11(2):93-8.
• Article: Fertility of Fusarium moniliforme from maize and sorghum related to fumonisin production in Italy.

  A Moretti, G A Bennett, A Logrieco, A Bottalico, M N Beremand
  [show abstract] [hide abstract]
  ABSTRACT: Forty-three strains of Fusarium moniliforme isolated from infected maize and sorghum plants in Italy were assayed for their ability to produce fertile crosses with "A" and "F" mating population tester strains, in relation to their ability to produce fumonisins on maize substrate. Most of the strains isolated from maize (ear and stalk rot and maize-based feed), producing fumonisin B1 (FB1) and B2 (FB2) (up to 4,100 and 855 mg/kg, respectively), belonged to the "A" mating population. All of the strains isolated from sorghum belonged to the "F" mating population and produced little or no FB1 and FB2. This is the first report of the occurrence of mating population "F" in Europe. Our data on strains from Italy are consistent with previous studies from the United States that found significant differences in sexual fertility and fumonisin production between strains from maize and sorghum.
  Mycopathologia 08/1995; 131(1):25-9. · 1.65 Impact Factor
• Article: Production of type A trichothecenes and enniatin B byFusarium sambucinum Fuckel sensu lato

  C. Altomare, A. Logrieco, A. Bottalico, G. Mulé, A. Moretti, A. Evidente
  [show abstract] [hide abstract]
  ABSTRACT: Twenty-nineFusarium isolates, representing three new taxa originated by Nirenberg fromF. sambucinum Fuckel sensu lato, namely:F. sambucinum Fuckel sensu stricto,F. venenotum Nirenb., andF. torulosum (Berk. & Curt.) Nirenb., were tested for in vitro production of toxic secondary metabolites on autoclaved corn kernels.F. sambucinum sensu stricto was able to produce type A trichothecenes and enniatin B (EB). In particular, amongst the 14 isolates tested, 5 produced only diacetoxyscirpenol (DAS) (up to 700 µg/g); 1 produced only neosolaniol (NEOS) (250 µg/g); 2 produced T-2 toxin (T-2) + NEOS (up to 175 and 150 µg/g, respectively); 1 produced NEOS + DAS (300 and 100 µg/g, respectively); and 5 produced DAS + EB (up to 500 and 140 µg/g, respectively). All six isolates ofF. venenotum were able to produce only DAS (up to 100 µg/g).F. torulosum produced no trichothecenes, but four out of nine tested isolates were able to produce EB (up to 140 µg/g). Zearalenones and type B trichothecenes were not found. The toxicity of the culture extracts towardsArtemia salina L. was correlated in general with the occurrence of the above toxins, except for someF. torulosum strains. However, the lack of correlation between the amounts of toxins recovered and toxic activity observed in theGeotrichum candidum Link ex Pers. andA. salina assays suggested the presence of unknown toxic compounds. Key wordsBioassays-Enniatin B- Fusarium -Trichothecenes
  Mycopathologia 04/1995; 129(3):177-181. · 1.65 Impact Factor
• Article: Phylogenetic relationship withinFusarium sambucinum Fuckel sensu lato, determined from ribosomal RNA sequences

  A. Logrieco, S. W. Peterson, A. Bottalico
  [show abstract] [hide abstract]
  ABSTRACT: Partial ribosomal RNA nucleotide sequences were determined for 11 strains ofFusarium sambucinum Fuckelsensu lato to assess by molecular genetic means, Nirenberg's recent morphotaxonomic interpretation which split the species into three distinct taxa:F. sambucinum sensu stricto, F. torulosum, and one other species, as yet unnamed (Fusarium species nova). Four sequence patterns were identified among the 11 strains. Two sequences that varied at one site were found among strains ofF. sambucinum, strains ofF. torulosum andFusarium sp. nov. showed no intraspecific variation. Interspecific comparisons revealed nucleotide sequence differences of 3–9 substitutions in the ca. 240 nucleotide rRNA segment examined. Although interspecific differences are not large in terms of percent nucleotide substitution, they are much larger than the observed intraspecific variation and support the morphological interpretation distinguishing three taxa. When the data were analysed using parsimony and bootstrapping, the three taxon tree was well supported. The phylogenetic arrangement of these strains is congruent with secondary metabolite profile similarities. Key words Fusarium species-Molecular systematics-Ribosomal RNA sequences
  Mycopathologia 04/1995; 129(3):153-158. · 1.65 Impact Factor
• Article: Fusarium sp. FN-2B: a controversial strain genetically close toFusarium poae.

  G Mule, A Logrieco, A Bottalico
  [show abstract] [hide abstract]
  ABSTRACT: Fusarium strain Fn-2B, a trichothecene producingFusarium strain, first reported asF. nivale but with a very controversial identification, was reexamined genetically by nucleotide sequencing from a highly variable region of the large subunit (25-28S) rRNA (D2 region, ca. 220 nucleotides), and compared to the same region from species it was presumed to belong, in order to assess its phylogenetic affinity.Fusarium strain Fn-2B proved to be more closely related toF. poae NRRL-13637 showing only one heteromorphic site. In comparison to other fungal strains, Fn-2B showed 3,11, and 34 bases that differ fromF. sporotrichioides NRRL-3299,F. triclnctum NRRL-13636 andMicrodochium nivale NRRL-13934, respectively. This phylogenetic affinity between Fusarium strain Fn-2B and F. poae is well correlates with the production of trichothecene mycotoxins by the species.
  Mycotoxin Research 03/1995; 11(1):53-8.
• Article: Isolation and characterization of fusaproliferin, a new toxic metabolite from Fusarium proliferatum.

  A Ritieni, V Fogliano, G Randazzo, A Scarallo, A Logrieco, A Moretti, L Mannina, A Bottalico
  [show abstract] [hide abstract]
  ABSTRACT: A new toxic sesterterpene, named fusaproliferin, was purified from corn kernel cultures (120 mg/kg dry culture) of a strain of Fusarium proliferatum isolated from corn ear rot in northern Italy. The stain, designated ITEM-1494, also produced fumonisin B1 (1.500 mg/kg dry culture) and beauvericin (90 mg/kg dry culture), but not moniliformin. To monitor toxicity, the brine shrimp assay was used throughout the isolation procedure. Fusaproliferin had a molecular formula of C27H40O5, and it is the first sesterterpene isolated from a Fusarium species.
  Natural Toxins 02/1995; 3(1):17-20.
• Article: Phylogenetic relationship within Fusarium sambucinum Fuckel sensu lato, determined from ribosomal RNA sequences.

  A Logrieco, S W Peterson, A Bottalico
  [show abstract] [hide abstract]
  ABSTRACT: Partial ribosomal RNA nucleotide sequences were determined for 11 strains of Fusarium sambucinum Fuckel sensu lato to assess by molecular genetic means, Nirenberg's recent morphotaxonomic interpretation which split the species into three distinct taxa: F. sambucinum sensu stricto, F. torulosum, and one other species, as yet unnamed (Fusarium species nova). Four sequence patterns were identified among the 11 strains. Two sequences that varied at one site were found among strains of F. sambucinum, strains of F. torulosum and Fusarium sp. nov. showed no intraspecific variation. Interspecific comparisons revealed nucleotide sequence differences of 3-9 substitutions in the ca. 240 nucleotide rRNA segment examined. Although interspecific differences are not large in terms of percent nucleotide substitution, they are much larger than the observed intraspecific variation and support the morphological interpretation distinguishing three taxa. When the data were analysed using parsimony and bootstrapping, the three taxon tree was well supported. The phylogenetic arrangement of these strains is congruent with secondary metabolite profile similarities.
  Mycopathologia 02/1995; 129(3):153-8. · 1.65 Impact Factor