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ABSTRACT: In this study, (1)H NMR-based metabonomics was applied to evaluate the beneficial effects of cordycepin (3'-deoxyadenosine), a natural monomer compound, on endogenous metabolic profiles of liver and plasma from hyperlipidemic Syrian golden hamsters. Hyperlipidemia was successfully established in hamsters fed by a high-fat diet for 2 weeks. The hyperlipidemic hamsters were treated with an oral administration of simvastatin (2 mg kg(- 1)) or cordycepin (140 mg kg(- 1)) for consecutive 4 weeks. The metabolic profiles of plasma and intact liver tissues were established using (1)H NMR spectroscopy. The results showed higher contents of lipids (triglyceride and cholesterol), lactate, acetate, alanine, glutamine together with lower contents of choline-containing compounds (e.g. phosphocholine, phosphatidylcholine, and glycerophosphocholine), glucose, and glycogen in plasma and liver samples from hyperlipidemic hamsters than those in controls. Cordycepin afforded a little lipid-regulating activity on plasma but more beneficial effects on liver, implicating that cordycepin might have a protective effect on liver under fatty liver condition.
Journal of Asian natural products research 06/2011; 13(6):534-46. · 0.61 Impact Factor
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ABSTRACT: Three novel sesquinlignans, tatanans A (1), B (2), and C (3), have been isolated from the rhizomes of Acorus tatarinowii Schott. Their structures were established by spectroscopic techniques and single-crystal X-ray analysis. Tatanans A-C potently increase GK enzymatic activity with EC(1.5) values in the range of 0.16-1.85 μM. The potent GK activity and unique structural features of tatanans make them promising leads for therapeutic development of antihyperglycemic drugs.
The Journal of Organic Chemistry 03/2011; 76(7):2056-61. · 4.45 Impact Factor
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ABSTRACT: Two new stilbenoids, cis-ϵ-viniferin (3) and 2b, 14b-dehydro-bisresveratrol (4) were synthesized by photooxidation reaction of trans-ϵ-viniferin (2) prepared from tram-resveratrol (1). Pentamethoxyl trans-ϵ-viniferin (5) and pentamethoxyl cis-ϵ-viniferin (6) were also obtained by methylation of trans-ϵ-viniferin (2) with (MeO)2SO2. Their structures were elucidated on the basis of spectral evidence. Compounds 3 and 4 showed potent inhibition of TNF-α at concentrations of 10−-5 mol.L−1 with inhibitory ratios of 51.43% and 36.64%. respectively.
Chinese Journal of Chemistry 08/2010; 22(11):1350 - 1355. · 0.75 Impact Factor
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Jia-Ming Wang,
Guang-Zhi Ding,
Lei Fang,
Jun-Gui Dai,
Shi-Shan Yu, Ying-Hong Wang,
Xiao-Guang Chen,
Shuang-Gang Ma,
Jing Qu,
Song Xu,
Dan Du
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ABSTRACT: Thirteen new thiodiketopiperazines, epicoccin I (1), ent-epicoccin G (2), and epicoccins J-T (3-13), together with six known diketopiperazines (14-19), have been isolated from the endophytic fungus Epicoccum nigrum. The structures of 1, 2, and 10 were confirmed by X-ray crystallography, and the absolute configurations of 2, 4, 6, and 8 were assigned using Moshers' method. Compounds 2, 6, 12, and 17 showed potent activities in vitro against the release of beta-glucuronidase in rat polymorphonuclear leukocytes induced by platelet-activating factor, with IC(50) values of 3.07, 4.16, 4.95, and 1.98 microM, respectively. None of the 19 compounds exhibited detectable cytotoxic activities toward six tumor cell lines (A549, Be-l7402, BGC-823, HCT-8, HCT-116, and A2780) in the MTT assay.
Journal of Natural Products 07/2010; 73(7):1240-9. · 3.13 Impact Factor
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ABSTRACT: Phytochemical investigation of the stem bark of Morus cathayana led to the isolation and identification of six new compounds, cathayanons F-J (1-5) and cathayanin A (6), and two known compounds, cathayanins B-C (7-8). Their structures were elucidated by spectroscopic methods. Compounds 1, 2, 3, 5, and 7 exhibited weak activities against five human cancer cell lines, with IC(50) values ranging from 4.7 to 9.8 microg/ml.
Journal of Asian natural products research 06/2010; 12(6):505-15. · 0.61 Impact Factor
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ABSTRACT: There is a concern that much of the atmospheric pollution experienced in Beijing is regional in nature and not attributable to local sources. The objective of this study is to examine the contribution of sources outside Beijing to atmospheric pollution levels during Beijing 2008 Olympic Games. The observations of SO2, NO(x), O3, PM2.5 and PM10 were conducted from June 1 to September 30, 2008 in Xianghe, a rural site about 70 km southeast of Beijing. Sources and transportation of atmospheric pollution during the experiment were discussed with surface meteorology data and backward trajectories calculated using HYSPLIT model. The results showed that the daily average maximum (mean +/- standard deviation) concentrations of SO2, NO(x), O3, PM2.5, and PM10 during observation reached 84.4(13.4 +/- 15.2), 43.3 (15.9 +/- 9.1), 230 (82 +/- 38), 184 (76 +/- 42) and 248 (113 +/- 52) microg x m(-3), respectively. In particular, during the pollution episodes from July 20 to August 12, the hourly average concentration of O3 exceeded the National Ambient Air Quality Standard II for 46 h (9%), and the daily average concentration of PM10 exceeded the Standard for 11 d (46%); PM2.5 exceeded the US EPA Standard for 18 d (75%). The daily average concentrations of SO2, NO(x), O3, PM2.5 and PM10 decreased from 27.7, 18.6, 96, 90, 127 microg x m(-3) in June-July to 5.8, 13.2, 80, 60, 106 microg x m(-3) during Olympic Games (August-September), respectively. The typical diurnal variations of NO(x), PM2.5 and PM10 were similar, peaking at 07:00 and 20:00, while the maximum of O3 occurred between 14:00 to 16:00 local time. The findings also suggested that the atmospheric pollution in Xianghe is related to local emission, regional transport as well as the meteorological conditions. Northerly wind and precipitation are favorable for diffusion and wet deposition of pollutants, while sustained south flows make the atmospheric pollution more serious. The lead-lag correlation analysis during the pollution episodes from July 20 to August 12 showed that there are about 6-10 h (0.57 < r < 0.65, p = 0.01) of hourly average PM2.5 in Beijing lagging Xianghe, reaching the maximum at 8 h, which indicates that the real-time atmospheric PM2.5 database of Xianghe might provides early warning for the Beijing PM2.5 pollution events.
Huan jing ke xue= Huanjing kexue / [bian ji, Zhongguo ke xue yuan huan jing ke xue wei yuan hui "Huan jing ke xue" bian ji wei yuan hui.] 01/2010; 31(1):1-9.
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ABSTRACT: Bioassay-guided fractionation of the ethanolic extract of Senecio scandens led to the isolation of four new compounds 4, 5, 7, and 8, along with four known jacaranone analogs (1, 2, 3, 6). Their structures were elucidated on the basis of spectral and chemical evidence. Compound 7 was obtained as a tautomeric mixture of alpha/beta-epimer. The cytotoxic activities of these compounds were evaluated. Among these, compounds 5 and 8 showed potent cytotoxicities. The benzoquinone derivative, jacaranone ethyl ester (1), was the major cytotoxic constituent in this plant with IC(50)s at a range of 0.5-1.0 microg/ml against various tumor cell lines. The SAR of these jacaranone analogs (1-8), isolated from S. scandens, was also discussed.
Journal of Asian natural products research 02/2009; 11(1):63-8. · 0.61 Impact Factor
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ABSTRACT: Three new Diels-Alder type adducts cathayanons C (1), D (2), and E (3), together with one known compound sanggenon C (4), were isolated from the stem bark of Morus cathayana. Their structures were fully elucidated by spectroscopic and chemical methods. Compound 2 showed good anti-oxidation activity with the inhibitory rate of malondialdehyde being 88% at a concentration of 10(- 6) mol/l.
Journal of Asian natural products research 02/2009; 11(3):267-73. · 0.61 Impact Factor
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ABSTRACT: To identify dihydroflavonol glycoside isomers in Smilax glabra.
The sample was analyzed by HPLC-MS in combination with HPLC-1H-NMR.
Four dihydroflavonol glycoside isomers were identified as astilbin, neoastilbin, isoastilbin, neoisoastilbin.
The method is simple and rapid for the identification of dihydroflavonol glycosides in S. glabra.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 07/2008; 33(11):1281-4.
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ABSTRACT: A combination of electrospray ionization tandem mass spectrometry with high-performance liquid chromatography (HPLC/ESI-MSn), and hyphenation of liquid chromatography to nuclear magnetic resonance spectroscopy (HPLC/NMR), have been extensively utilized for on-line analysis of natural products, analyzing metabolite and drug impurity. In our last paper, we reported an on-line analytical method for structural identification of trace alkaloids in the same class. However, the structural types of the constituents in plants were various, such as flavanoids, terpenoids and steroids. It is important to establish an effective analytical method for on-line structural identification of constituents with molecular diversity in extracts of plants. So, in the present study, the fragmentation patterns of some isolated stilbenes, phloroglucinols and flavanoids from Lysidice rhodostegia were investigated by ESI-MSn. Their fragmentation rules and UV characteristics are summarized, and the relationship between the spectral characteristics, rules and the structures is described. According to the fragmentation rules, NMR and UV spectral characteristics, 24 constituents of different types in the fractions from L. brevicalyx of the same genus were structurally characterized on the basis of HPLC/HRMS, HPLC-UV/ESI-MSn, HPLC/1H NMR and HPLC/1H-1H COSY rapidly. Of these, six (10, 13, 14, 16, 17 and 23) are new compounds and all of them are reported from L. brevicalyx for the first time. The aim is to develop an effective analytical method for on-line structural identification of natural products with molecular diversity in plants, and to guide the rapid and direct isolation of novel compounds by chemical screening.
Rapid Communications in Mass Spectrometry 02/2008; 22(6):755-65. · 2.79 Impact Factor
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ABSTRACT: Two new lignan glucosides, compounds 2 and 3, two new 1H-indole-alkaloid glucosides, 5 and 6, as well as two new phenolic glucosides, 7 and 10, were isolated from the roots of Capparis tenera, together with five known compounds. Their structures were characterized by chemical and spectroscopic methods. Most of these isolates were obtained for the first time from Capparidaceae. The antioxidant and anti-inflammatory activities of the new compounds were investigated.
Chemistry & Biodiversity 01/2008; 4(12):2852-62. · 1.80 Impact Factor
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ABSTRACT: Bioassay-guided fractionation of 95% EtOH extract from the roots of Dipsacus asper lead to the isolation of some phenolic acids (caffeic acid, 2,6-dihydroxycinnamic acid, vanillic acid, 2'-O-caffeoyl-D-glucopyranoside ester, and caffeoylquinic acid) as the major active components, and five new iridoid glucoside dimers (1-5) and one new iridoid glucoside monomer (6), other known iridoid glycosides loganin, cantleyoside, triplostoside A, lisianthioside, 6'-O-beta-D-apiofuranosyl sweroside, as well as triterpenoids oleanic acid and akebiasaponin D. The structures of new compounds 1-6 were determined as dipsanosides C (1), D (2), E (3), F (4), G (5), and 3'-O-beta-D-glucopyranosyl sweroside (6) by spectroscopic, including 1D and 2D NMR techniques, and chemical methods.
CHEMICAL & PHARMACEUTICAL BULLETIN 01/2008; 55(12):1677-81. · 1.59 Impact Factor
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ABSTRACT: To study the chemical constituents from the roots of Cudrania cochinchinensis.
The chemical constituents of C. cochinchinensis were isolated and purified by silica gel, polyamide column chromatography, Their structures were identified on the basis of spectroscopic data.
Six compounds were isolated and identified. Their structures were established as 3, 5, 7, 4"-tetrahydroxyflavanone-7-O-(6"-acetyl)-glucoside (1), 3, 5, 7, 4'-tetra hydroxyflavanone-7-O-glucoside (2), 2', 4', 5, 7-tetrahydroxy-6-prenyldihydroflavanone (3), 5, 7, 4'-trihysdroxy-6-prenylisoflavanone (4), 1, 3, 5, 6-tetrahydroxyxanthone (5), stilbene-2, 4, 3', 5'-tetraol (6).
Compound 1 was isolated from this genus, while compounds 4 and 5 were isolated from this plant for the first time.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 04/2007; 32(5):406-9.
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ABSTRACT: Electrospray ionization multi-stage tandem mass spectrometry (ESI-MSn), and combination with HPLC (HPLC/ESI-MSn), have been extensively applied to on-line analysis of natural products. Hyphenation of liquid chromatography to nuclear magnetic resonance spectroscopy (HPLC/NMR) has been developed in the last decade, which is utilized for the analysis of metabolites and drug impurities. In the study reported here, the fragmentation behaviors of eight cassaine-type diterpenoid amides from Erythrophleum fordii were investigated by ESI-MSn. The fragmentation rules and NMR spectral characteristics are summarized, and the relationship among the rules, characteristics and the structures is described. According to the fragmentation rules and NMR spectral characteristics, seven trace constituents and two formerly obtained compounds of cassaine-type diterpenoid amides in the fractions from E. fordii were structurally characterized on the basis of HPLC/HRMS, HPLC-DAD/ESI-MSn, HPLC/1H NMR and HPLC/1H-1H COSY rapidly. Among them, constituents 1-5 are new compounds, and 6 and 7 are reported from E. fordii for the first time. The aim is to develop an effective analytical method for structural identification of new trace natural products in plants.
Rapid Communications in Mass Spectrometry 02/2007; 21(13):2109-19. · 2.79 Impact Factor
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ABSTRACT: [structure: see text] Two unusual glycosidic triterpene alkaloids, machilaminosides A (1) and B (2), have been isolated from the stem barks of Machilus yaoshansis. Their structures were elucidated by detailed spectroscopic analysis. A possible biogenetic origin of 1 and 2 mediated by the coupling of 2-O-beta-D-glucopyranosyl-cucurbitacin I, respectively, with urea and adenosine was postulated. 1 and 2 showed nonselective cytotoxic activities against several human cancer cell lines as well as TNF-alpha secretion inhibitory activities.
Organic Letters 02/2007; 9(1):129-32. · 5.86 Impact Factor
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ABSTRACT: To study the chemical constituents of Bauhinia aurea.
The compounds were isolated with column chromatography. The structures were determined by MS and NMR spectroscopic techniques.
Nine aromatic acid derivatives, two triterpenes and three steroids were isolated from the 90% ethanolic extract and their structures were identified as 3,4-di-hydroxybenzoic acid (1), 4-hydroxybenzoic acid (2), 3-hydroxy-4-methoxy-benzoic acid (3), 4-hydroxy-3-methoxybenzoic acid (4) , gallic acid (5) , methyl gallate (6) , ethyl gallate (7) , lupeol (8) , lupenone (9) , stigmast4-en-3-one (10) beta-sitosterol (11) and daucosterol (12).
This is the first report of chemical constituents from B. aurea. Compounds were isolated from this genus for the first time, except 5,8,11 and 12.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 01/2007; 31(23):1953-5.
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ABSTRACT: [reaction: see text] Two new iridoid glucoside tetramers, dipsanosides A (1) and B (2), the first-reported iridoid tetramers with four glucosides, were isolated from Dipsacus asper. Their structures were determined by analysis of 1D and 2D NMR data as well as by comparison with model compounds. Their cytotoxicities were tested, but neither of them showed obvious activity.
Organic Letters 06/2006; 8(10):2179-82. · 5.86 Impact Factor
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ABSTRACT: Four acetylated flavonol diglucosides, quercetin 3-O-[2'''-O-acetyl-beta-d-glucopyranosyl-(1-->6)-beta-d-glucopyranoside], quercetin 3-O-[2''',6'''-O-diacetyl-beta-d-glucopyranosyl-(1-->6)-beta-d-glucopyranoside], isorhamnetin 3-O-[2'''-O-acetyl-beta-d-glucopyranosyl-(1-->6)-beta-d-glucopyranoside], and quercetin 3-O-[2'''-O-acetyl-alpha-l-arabinopyranosyl-(1-->6)-beta-d-glucopyranoside], together with five known flavonol glycosides quercetin 3-O-beta-d-glucopyranoside, kaempferol 3-O-beta-d-glucopyranoside, quercetin 3-O-[beta-d-galactopyranosyl-(1-->6)-glucopyranoside], isorhamnetin 3-O-[beta-d-galactopyranosyl-(1-->6)-beta-d-glucopyranoside], and kaempferol 3-O-[beta-d-glucopyranosyl-(1-->2)-beta-d-glucopyranoside] have been isolated from Meconopsis quintuplinervia. Their structures were determined using chemical and spectroscopic methods including HRFABMS, (1)H-(1)H COSY, HSQC and HMBC experiments.
Phytochemistry 04/2006; 67(5):511-5. · 3.35 Impact Factor
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ABSTRACT: Combining improved injector, gas line and valve-driving modules, a GC equipped with FID and ECD, could simultaneously measure CH4, CO2 and N2O in an air sample within 4 min. Test results showed that the system has high sensitivity, resolution and precision; the linear response range of the system meets the requirement of in situ flux measurements. Thus, the system is suitable for monitoring fluxes of main greenhouse gases in terrestrial ecosystem since it is easy to use, efficacious, stable and reliable to collect data.
Journal of Environmental Sciences 01/2006; 18(1):162-9. · 1.66 Impact Factor
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ABSTRACT: Four new iridoid glycosides, 10-O-trans-p-coumaroylscandoside (1), 10-O-cis-p-coumaroylscandoside (2), 10-O-trans-p-coumaroyldesacetyl asperulosidic acid (3), and 10-O-cis-p-coumaroyldesacetyl asperulosidic acid (4), and two new grayanane diterpenoids, 14beta-O-(2S,3S-nilyl)-2alpha,3beta,5beta,6beta,16alpha-pentahydroxygrayanane (5) and 14beta-O-(2S,3S-nilyl)-2alpha,3beta,5beta,6beta,16alpha-pentahydroxygrayan-10(20)-ene (6), have been isolated from Craibiodendron henryi. The structures of these compounds were determined by chemical and spectroscopic methods including 1H-1H COSY, HMQC, HMBC, and NOESY experiments. Antioxidant activities and vasodilator effects of these compounds were assessed.
Journal of Natural Products 12/2005; 68(11):1646-50. · 3.13 Impact Factor
