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J Lubiński,
B Górski,
T Huzarski,
T Byrski,
J Gronwald,
P Serrano-Fernández, W Domagała,
M Chosia,
M Uciński,
E Grzybowska, [......],
J Ryś,
M Szwiec,
A Rozmiarek,
I Dziuba,
P Wandzel,
R Wiśniowski,
C Szczylik,
A Kozak,
W Kozłowski,
S A Narod
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ABSTRACT: We identified 4316 unselected incident cases of early-onset breast cancers (<51 ears of age at diagnosis) in 18 Polish hospitals between 1996 and 2003. We were able to obtain a blood sample for DNA analysis from 3472 of these (80.4%). All cases were tested for the presence of three founder mutations in BRCA1. The proportion of cases with a BRCA1 mutation was 5.7%. The hereditary proportions were higher than this for women with breast cancer diagnosed before age 40 (9%), for women with cancer of medullary or atypical medullary histology (28%), for those with bilateral cancer (29%) or with a family history of breast or ovarian cancer (13%). It is reasonable to offer genetic testing to women with early-onset breast cancer in Poland.
Breast Cancer Research and Treatment 09/2006; 99(1):71-6. · 4.43 Impact Factor
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ABSTRACT: In many European countries the morbidity and mortality due to cancer of the cervix are too high. Even in countries, where smears have been taken at regular intervals for many years, the percentage of new cases does not decrease as much as it was expected. There are several reasons that may explain this situation. At least two of them can be solved by pathologists: quality control (QC) system and organization of a school of gynaecological cytopathology for pathologists and cytotechnicians. Here we focus on the main aspects of External and Internal QC and new approaches to QC, including new methods and stricter control of the professional level of all persons involved in the screening and diagnosis of cervical cancer.
Polish journal of pathology: official journal of the Polish Society of Pathologists 02/2000; 51(2):87-91. · 0.35 Impact Factor
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ABSTRACT: The purpose of this study was to correlate the presence of p53 antibodies in sera of patients with colorectal adenocarcinoma with size, site and stage of the tumour, age and sex of a patient and the level of carcinoembryonic antigen (CEA) in the serum. p53 antibodies were detected using enzyme-linked immunoabsorbent assay (ELISA). Serum p53 antibodies were detected in 30 of 145 patients (21%), mostly in Astler-Coller stage B1 (28% of patients). No association was found between p53 antibody status in stage A+B1+B2 vs stages C1+C2+D (22% vs 19%) i.e. between patients without and with metastases to regional lymph nodes and/or distant metastases. Serum p53 antibodies were detected in 9 of 34 patients (26%) with tumour localised in the right part vs 21 of 109 patients (19%) with tumours in the left part of the colon and in 18 of 96 (19%) of patients with tumours localised in rectosigmoideum vs 12 of 47 (26%) with tumours in the remaining colon. There was no significant correlation between serum anti p53 antibody and CEA statuses. Increased level of serum CEA was seen in 46/145 (32%) patients. Patients with C1+C2+D stage cancers had high serum CEA level more frequently than did patients with A+B1+B2 stage tumours (44% vs 19% respectively, p < 0.001). Of 102 cases with normal CEA level, 19 (19%) were positive for anti p53 antibodies. These results together with the literature data [11, 20] indicate that approximately 27% CEA negative patients may have serum p53 antibodies. Therefore simultaneous assessment of serum p53 antibodies and CEA seems to be useful for monitoring high risk patients and for postoperative patient monitoring.
Polish journal of pathology: official journal of the Polish Society of Pathologists 01/1999; 50(2):77-81. · 0.35 Impact Factor
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ABSTRACT: The purpose of this study was the evaluation of nuclear area, nuclear axis ratio, perimeter and roundness of nuclei of tumor cells with and without Ki-67 antigen expression (demonstrated immunohistochemically using MIB-1 antibody) in primary and metastatic malignant melanoma of the skin. The parameters were further analyzed with respect to their association with the depth of malignant invasion according to Clark [7] and tumor thickness according to Breslow [6]. 142 malignant melanomas (53 primary and 89 metastatic), were assessed employing a computerized image analyzer Quantimet 600S (Leica). The mean nuclear area of MIB-1 positive nuclei was significantly larger than that of the negative ones (p < 0.0001) both in primary and metastatic malignant melanoma. In comparison to the primary melanoma the nuclei of metastatic melanoma cells had a larger area and were more rounded, while the MIB-1 positive nuclei additionally showed a greater degree of polymorphism of their area and shape. With growing invasion thickness according to Breslow and increased Clark's level, the mean nuclear area of tumor cells increased, and their shape became more round. The MIB-1 positive tumor cell nuclei of primary melanomas with metastases were significantly out of round in comparison to primary melanomas without metastases. The results indicate an association between the area and shape of melanoma cell nuclei and the presence of metastases, and between the nuclear area of tumor cells and such factors related to poor prognosis as the depth of invasion and the tumor thickness.
Polish journal of pathology: official journal of the Polish Society of Pathologists 01/1999; 50(4):235-41. · 0.35 Impact Factor
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ABSTRACT: The relationship between proliferating cell nuclear antigen (PCNA) expression and clinical stage, lymph node status, histological grade, malignancy index (MI) [13] were studied in 103 laryngeal squamous cell carcinomas (SCC). The PCNA index (the percentage of PCNA positive nuclei) was examined immunohistochemically using monoclonal PC10 antibody on paraffin sections. PCNA immunoreactivity was seen in all samples with mean value of PCNA index 37.4% for supraglottic and 36.8% for glottic SCC. The PCNA index was significantly related to histological grade and malignancy index (p < 0.05). For all tumors no correlation was found between PCNA index and clinical stage and lymph node status however, metastasizing glottic SCC had higher PCNA index than non-metastasizing ones. The modification of MI in glottic laryngeal SCC by adding PCNA index as a new parameter is proposed.
Polish journal of pathology: official journal of the Polish Society of Pathologists 02/1996; 47(4):183-7. · 0.35 Impact Factor
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ABSTRACT: MIB-1 Ki-67 and PCNA scores in infiltrating ductal NOS breast carcinomas were compared. The correlation between MIB-1, Ki-67 and PCNA indices and several clinicopathological factors that have prognostic significance in breast cancer was also assessed. The mean Ki-67, MIB-1 and PCNA indices were 13.4%, 19.4%, 27.6%, respectively. Significant positive linear correlation was found only between Ki-67 and MIB-1 indices. PCNA score did not correlate with Ki-67 and MIB-1 indices. The significant correlation between Ki-67 and MIB-1 scores and histological grade was found. There was no correlation between Ki-67 and MIB-1 indices and axillary lymph node status or tumor diameter. The results suggest that MIB-1 antibody is an excellent tool for assessment of proliferative rate of breast cancer cells in paraffin sections.
Polish journal of pathology: official journal of the Polish Society of Pathologists 02/1996; 47(4):189-94. · 0.35 Impact Factor
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ABSTRACT: We analyzed the expression of proliferating cell nuclear antigen (PCNA) in ectocervical sections fixed in formalin and embedded in paraffin using monoclonal antibody PC10, PAP and APAAP methods. A total of 156 cases was studied: 32 cases with normal epithelium, 33 cases with CIN I, 36 cases with CIN II, 55 cases with CIN III. We evaluated: 1. the index of PCNA (IPCNA), i.e. the proportion of PCNA-positive cells relative to all cells in the epithelium; 2. the IPCNA in each epithelial layer; 3. the epithelial height with PCNA-positive cells relative to the total height of the epithelium; 4. changes in the IPCNA with respect to the presence or absence of histological signs of HPV infection. We found: 1. a significant correlation between the index of PCNA and the severity of dysplasia in the whole epithelium and its individual layers; 2. a significant correlation between the epithelial height with PCNA-positive cells relative to the total height of the epithelium and the severity of dysplasia; 3. a lack of correlation between the IPCNA and HPV(+) and HPV(-) cases.
Polish journal of pathology: official journal of the Polish Society of Pathologists 02/1996; 47(4):171-4. · 0.35 Impact Factor
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ABSTRACT: Monoclonal anti-proliferating cell nuclear antigen antibody (PCNA, PC 10) was used to measure proliferation of tumor cells and stromal cells in 27 colorectal adenocarcinomas. Mean proliferation index of cancer cells was 33.7% (SD +/- 16.3) and that of stromal cells 14.3 (SD +/- 12.5). Positive correlation between proliferation index of cancer cells and proliferation rate of stromal cells was found (r = 0.64, p < 0.001). Correlation between proliferation of stromal lymphocyte-like cells and fibroblast-like cells was noticed (r = 0.69, p < 0.001).
Polish journal of pathology: official journal of the Polish Society of Pathologists 01/1996; 47(3):101-4. · 0.35 Impact Factor
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ABSTRACT: Cathepsin D expression was determined by immunohistochemistry in 445 formalin fixed, paraffin embedded primary invasive breast carcinomas. We found: 1. a relationship between cathepsin D expression and histological type of tumour, 2. a negative correlation between cathepsin D expression and histological grade, 3. a negative correlation between cathepsin D expression and tumour diameter, 4. a relationship between the morphology of cathepsin D granules and histological type of the tumour.
Polish journal of pathology: official journal of the Polish Society of Pathologists 02/1995; 46(1):11-5. · 0.35 Impact Factor
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ABSTRACT: Proliferative activity of epithelial tumor cells was evaluated with the use of immunohistochemistry and anti-PCNA monoclonal antibodies in alcohol fixed paraffin embedded sections of 44 colonic adenomas, including 33 tubular, 5 villous and 6 tubulovillous adenomas. The mean PCNA index was 24.7 +/- 10.9%, 24.8 +/- 6.2% and 24.8 +/- 14.0% in tubular, villous and tubulovillous adenomas respectively. In 12 tubular adenomas with dysplasia the mean PCNA index in areas with dysplasia was significantly higher (38.2 +/- 11.5%) as compared to areas without dysplasia (17.0 +/- 8.9%; p < 0.05). The results indicate that PCNA index of epithelial tumor cells is significantly increased in adenomas with high grade of dysplasia irrespective of histological type or size of the tumour.
Polish journal of pathology: official journal of the Polish Society of Pathologists 01/1995; 46(3):151-4. · 0.35 Impact Factor
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ABSTRACT: Proliferating cell nuclear antigen (PCNA/cyclin) is a useful marker of proliferation and its expression correlates with prognosis in some human neoplasms. The clinical course of nodular sclerosis Hodgkin's disease (NSHD) varies in both grades (NSI, NSII) as defined by the British National Lymphoma Investigation. Expression of PCNA in Reed-Sternberg cells and their variants was evaluated in NSI and NSII. There were no significant differences in the ratio of PCNA-positive cells in both groups. The results suggest that differences in the clinical course do not depend on the proliferative rate of Reed-Sternberg cells and their variants in NSHD.
Patologia polska 02/1993; 44(3):129-31.
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ABSTRACT: Monoclonal antibodies to keratin, vimentin, leukocyte common antigen (LCA) and S-100 protein have been used in fine needle aspirates of 35 metastatic malignant melanomas, 136 carcinomas, 35 sarcomas and 82 non-Hodgkin's lymphomas in search for immunocytochemical criteria useful in differential diagnosis of melanoma versus carcinoma, non-Hodgkin's lymphoma and sarcoma. All melanomas expressed vimentin and did not express keratin. Six of 14 melanomas contained S-100 protein. All carcinomas were keratin positive. Some were also vimentin positive. All sarcomas expressed vimentin. Synovial sarcomas were also keratin positive. All NHLs were vimentin positive, keratin negative. All NHLs except one expressed also LCA. It is concluded that keratin, vimentin and LCA are useful markers in differential diagnosis of malignant melanoma versus carcinoma and non-Hodgkin's lymphoma in fine needle aspirates when used together with morphologic and clinical data. However, in differential diagnosis of malignant melanoma and sarcoma these markers are of little use.
Patologia polska 02/1991; 42(3):73-8.
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ABSTRACT: Immunocytochemistry with the use of monoclonal antibodies to vimentin, keratin, desmin, neurofilaments and leukocyte common antigen (LCA) was applied to 53 fine needle aspirates of small cell-, round-, blue-cell malignant tumors of childhood (neuroblastomas, nephroblastomas, non-Hodgkin's lymphomas, Ewing's sarcomas and rhabdomyosarcomas). The results revealed the following immunophenotypes: neuroblastomas were positive for neurofilaments. Nephroblastomas contained vimentin (in blastema cells), keratin (in epithelial and blastema cells) Non-Hodgkin's lymphomas were vimentin and LCA positive, keratin negative. Ewing's sarcomas displayed multidirectional differentiation. Rhabdomyosarcomas were desmin and vimentin positive. It is concluded that immunocytochemistry in fine needle aspirates should be used as an objective evidence to support morphologic differential diagnosis of small cell-, round-, blue cell malignant tumors of childhood.
Patologia polska 02/1991; 42(3):79-82.
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ABSTRACT: The results of application of transmission electron microscopy to the differential diagnosis of malignant tumors in needle aspirates are summarized. The analysis is based on the personal experience of the authors with 117 cases and the review of 223 cases published in the literature. The following four topics are discussed in detail: 1. Preparation of aspirates for transmission electron microscopic examination-limitations and advantages of various technical approaches. 2. Ultrastructural characteristics of malignant cells aspirated from various types of human tumors. 3. The diagnostic significance of ultrastructural examination of tumor cells in aspirates. 4. Transmission electron microscopy as compared to immunocytochemistry and to other methods used to aid differential diagnosis of needle aspirates. It is concluded that transmission electron microscopy when properly introduced into the diagnostic process helps to resolve many difficult problems of differential diagnosis of tumors in needle aspirates.
Patologia polska 02/1989; 40(1):39-68.
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Polskie archiwum medycyny wewnȩtrznej 01/1983; 68(6):413-9. · 1.37 Impact Factor
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Patologia polska 39(1):43-54.
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Patologia polska 36(2):195-9.
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Patologia polska 37(3):197-204.
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Patologia polska 39(1):34-42.
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Patologia polska 39(1):55-65.
