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ABSTRACT: Kinetics of 4-aminobensenesulphonate (4-ABS) degradation by Pannonibacter sp. W1 under aerobic condition was studied. At medium and low initial concentration (50-1000 mg/L), the 4-ABS could be degraded almost completely in 14 h,and the degradation reaction conformed to one order kinetics. At high initial concentration (1 200-2500 mg/L), up to 90% degradation rate could be achieved in 32 h, and it conformed to zero order kinetics in earlier period of degradation and one order kinetics in later period of degradation. Haldane inhibition model was used to fit the special degradation rate at different initial concentration, and the parameters micro(max), K(s) and K(i) were determined to be 227.977 mg/(g x h), 84.306 mg/L and 1270.675 mg/L, respectively. UV spectrum and HPLC detection indicated that no other aromatic intermediates were accumulated during the 4-ABS degradation. The degradation of other substituted benzenes by strain W1 were detected, and the metabolic pathway of 4-ABS degradation was proposed.
Huan jing ke xue= Huanjing kexue / [bian ji, Zhongguo ke xue yuan huan jing ke xue wei yuan hui "Huan jing ke xue" bian ji wei yuan hui.] 07/2009; 30(7):2136-41.
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ABSTRACT: 4-Aminobenzenesulfonate (4-ABS) is a representative intermediate of some sulfonated azo dyes. A novel Pannonibacter sp. strain W1 capable of degrading 4-ABS as sole carbon as well as energy, nitrogen and sulfur source was isolated and identified from the activated sludge of a municipal wastewater treatment plant. Strain W1 was able to completely degrade 4-ABS with initial concentrations of 200-2500 mg L(-1) within 40 h. Haldane inhibition model was used to fit the special degradation rate at different initial concentrations, and the parameters micro(max), K(s) and K(i) were determined to be 227.977 mg (gh)(-1), 84.306 mg L(-1) and 1270.675 mg L(-1), respectively. Elements N and S of 4-ABS were released in respective forms of ammonia and sulfate while degrading 4-ABS by strain W1, accounting for 77.6% and 92.2% of theoretical values, respectively. Relatively low recoveries of dissolved N and S were probably owing to the fact that part of the released ammonia and sulfate were utilized by strain W1 for cell growth. It was found that the reduction of total organic carbon (TOC) was proportional to the degradation of 4-ABS and 84.4% TOC removal rate, corresponding to a 4-ABS degradation rate of 94.7%, was achieved at the end of the test. Additionally, HPLC and UV analyses indicated that there were no other aromatic intermediates detectable, suggesting the achievement of a complete mineralization of 4-ABS with strain W1.
Journal of hazardous materials 05/2009; 169(1-3):1163-7. · 4.14 Impact Factor
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ABSTRACT: A 4-aminobenzenesulphonate-degrading strain W1 was isolated from the activated sludge of Dalian municipal wastewater treatment plant. Strain W1 was able to utilize 4-ABS as sole carbon and energy source under aerobic condition. It was identified as Pannonibacter sp. according to its morphological, physiological and biochemical characteristics and the analysis of its 16S rDNA gene. The optimum conditions for 4-ABS biodegradation in the shaking flasks were 10% inoculum, 30 degrees C, pH 7.0 and were rotated at speed 150 r/min, respectively. Strain W1 could keep high degrading ability even in the presence of extra carbon source. Release of ammonia and sulfate were around 77.6% and 91.5% of theoretic concentration according to 4-ABS degradation, for 4-ABS could serve as the nitrogen and sulfur source. Strain W1 showed efficient biodegradability even at 2,500 mg/L, and 90% 4-ABS removal was achieved in 32 h. The 84.4% TOC removal was achieved after 94.7% consumption of 4-ABS, and no aromatic intermediates were detected, referring the complete mineralization of 4-ABS by strain W1.
Huan jing ke xue= Huanjing kexue / [bian ji, Zhongguo ke xue yuan huan jing ke xue wei yuan hui "Huan jing ke xue" bian ji wei yuan hui.] 05/2009; 30(4):1193-8.
