E Ferrary

Unité Inserm U1077, Caen, Lower Normandy, France

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Publications (151)252.4 Total impact

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    ABSTRACT: Menière's disease, clinically characterized by fluctuating, recurrent, and invalidating vertigo, hearing loss, and tinnitus, is linked to an increase in endolymph volume, the so-called endolymphatic hydrops. Since dysregulation of water transport could account for the generation of this hydrops, we investigated the role of aquaporin 3 (AQP3) in water transport into endolymph, the K-rich, hyperosmotic fluid that bathes the apical ciliated membrane of sensory cells, and we studied the regulatory effect of dexamethasone upon AQP3 expression and water fluxes. The different AQP subtypes were identified in inner ear by RT-PCR. AQP3 was localized in human utricle and mouse inner ear by immunohistochemistry and confocal microscopy. Unidirectional transepithelial water fluxes were studied by means of (3)H2O transport in murine EC5v vestibular cells cultured on filters, treated or not with dexamethasone (10(-7) M). The stimulatory effect of dexamethasone upon AQP3 expression was assessed in EC5v cells and in vivo in mice. AQP3 was unambiguously detected in human utricle and was highly expressed in both endolymph secretory structures of the mouse inner ear, and EC5v cells. We demonstrated that water reabsorption, from the apical (endolymphatic) to the basolateral (perilymphatic) compartments, was stimulated by dexamethasone in EC5v cells. This was accompanied by a glucocorticoid-dependent increase in AQP3 expression at both messenger RNA (mRNA) and protein level, presumably through glucocorticoid receptor-mediated AQP3 transcriptional activation. We show that glucocorticoids enhance AQP3 expression in human inner ear and stimulate endolymphatic water reabsorption. These findings should encourage further clinical trials evaluating glucocorticoids efficacy in Menière's disease.
    Pflugers Archiv : European journal of physiology. 10/2014;
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    ABSTRACT: Loading otoprotective drug into cochlear implant might change its mechanical properties, thus compromising atraumatic insertion. This study evaluated the effect of incorporation of dexamethasone (DXM) in the silicone of cochlear implant arrays on insertion forces.
    08/2014;
  • ARO 2014; 01/2014
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    ABSTRACT: But de la présentation L’objectif est d’améliorer les performances et le confort d’écoute des adultes implantés depuis au moins un an (Advanced Bionics) par une optimisation du réglage proposée par le logiciel FOX® (Fitting Outcoume Expert, Govaerts et al., Eargroup, Anvers). Matériel et méthodes Dix-huit adultes (9 hommes, 9 femmes), dont trois patients implantés bilatéralement (21 oreilles testées), âgés de 54 ± 17 ans (moyenne ± ET) [extrêmes : 28–78 ans] à la date de l’évaluation, et ayant un délai post-implantation de 5 ± 2 ans [2–9], ont participé à l’étude. L’évaluation se déroule en deux sessions d’environ deux heures (1 itération de réglage FOX®), à deux mois d’intervalle. Une audiométrie tonale (250 à 8000 Hz), quatre listes cochléaires de Lafon (40, 55, 70 et 80 dB), deux listes de phrases MBAA dans le bruit (S/B : 5 dB et 10 dB), un test de discrimination de 20 paires de phonèmes et une évaluation de la croissance en sonie (à 250, 1000 et 4000 Hz) ont été proposés en champ libre. Le questionnaire APHAB a également été complété. Résultats Deux mois après le réglage FOX®, les scores moyens de reconnaissance de phonèmes (Lafon) à 40 dB et 55 dB sont améliorés par rapport aux performances initiales : 4 % à la première session versus 14 % (p < 0,05) à la seconde session à 40 dB, et 33 % versus 47 % (p < 0,05) à 55 dB. À 55 dB, parmi les douze oreilles ayant les scores les plus faibles (< 40 %) lors de la première sessions six, soit la moitié, s’améliorent d’au moins 15 %. Par contre, parmi les 9 oreilles ayant des résultats supérieurs à 40 %, seule une s’améliore d’au moins 15 %. Le score global de l’APHAB (58 % vs 53 %, p < 0,05) ainsi que les domaines « facilité de communication » (45 % vs 39 %, p < 0,05) et « bruit de fond » (66 % vs 56 %, p < 0,05) sont également améliorés. Les scores aux autres évaluations ne sont ni améliorés ni dégradés. Conclusion L’utilisation du logiciel FOX® pour le réglage des implants cochléaires Advanced Bionics est possible en routine clinique et permet d’améliorer, chez certains patients, la compréhension de la parole à faible intensité et le confort d’écoute, et cela même à distance de l’implantation. Les patients avec les plus faibles résultats semblent obtenir davantage de bénéfices de cette méthode de réglage que les autres patients.
    Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale. 01/2014; 131(4):A9–A10.
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    ABSTRACT: But de la présentation Les performances cognitives des personnes de plus de 60 ans présentant une perte auditive supérieure à 25 dB sont inférieures à celles des normo-entendants (Lin, 2013). Plus particulièrement, le seuil d’audition des presbyacousiques est relié aux performances exécutives (Gates, 2010). L’objectif de cette étude est de déterminer si les capacités cognitives des patients presbyacousiques, évaluées avec les tests Codex et MoCA, sont différentes de celles des sujets normo-entendants de même âge. Matériel et méthodes Les performances auditives de 40 patients adultes, 21 femmes et 19 hommes, d’âge moyen 61,1 ± 15,36 ans (ES = 2,43) [22–88] présentant une surdité profonde depuis 55 ± 66 mois [2–264] ont été testées avec des listes de syllabes, de mots monosyllabiques de Lafon, de mots dissyllabiques de Fournier et de phrases MBAA proposées en modalité auditive. Les tests cognitifs de dépistage Codex et MoCA ont été proposés en modalité visuelle. Résultats L’analyse des résultats des tests Codex et MoCA chez les patients sourds ne révèle aucune corrélation avec les performances auditivo-verbales. Les résultats sont anormaux chez 10 patients (25 %) pour le Codex et chez 21 patients (53 %) pour le MoCA, 10 patients ayant des résultats anormaux aux deux tests. L’épreuve d’orientation, déterminante dans le Codex pour départager les patients pathologiques ou normaux – et commune aux deux tests, s’avère non discriminante. Par ailleurs, les épreuves du MoCA les moins bien réussies sont celles testant les fonctions exécutives et la mémoire épisodique. Elles sont corrélées à un score total pathologique au MoCA : capacités visuo-spatiales exécutives (r = 0,95, p < 0,0001),catégorisation (r = 0,95, p < 0,0001) et rappel (r = 0,95, p < 0,0002). Ces épreuves apparaissent donc discriminantes chez les patients sourds. Enfin, ces deux tests n’explorent pas les mêmes fonctions. Conclusion Vingt-cinq pour cent des patients sourds de notre étude ont des scores pathologiques au CODEX et 52,5 % au MoCA. Le profil cognitif des sujets presbyacousiques est différent des normo-entendants, le MoCA ayant été étalonné sur 90 sujets contrôles normo-entendants d’âge moyen 72,84 ± 7,03 ans. Les tests cognitifs de dépistage MoCA et Codex,simples et rapides, pourront être utilisés dans le suivi longitudinal des sujets implantés cochléaires afin de confirmer l’amélioration des fonctions cognitives grâce à la réhabilitation auditive.
    Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale. 01/2014; 131(4):A12.
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    ABSTRACT: Otological microsurgery is delicate and requires high dexterity in bad ergonomic conditions. To assist surgeons in these indications, a teleoperated system, called RobOtol, is developed. This robot enhances gesture accuracy and handiness and allows exploration of new procedures for middle ear surgery. To plan new procedures that exploit the capacities given by the robot, a surgical simulator is developed. The simulation reproduces with high fidelity the behavior of the anatomical structures and can also be used as a training tool for an easier control of the robot for surgeons. In the paper, we introduce the middle ear surgical simulation and then we perform virtually two challenging procedures with the robot. We show how interactive simulation can assist in analyzing the benefits of robotics in the case of complex manipulations or ergonomics studies and allow the development of innovative surgical procedures. New robot-based microsurgical procedures are investigated. The improvement offered by RobOtol is also evaluated and discussed.
    BioMed Research International 01/2014; 2014:891742. · 2.71 Impact Factor
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    ABSTRACT: Introduction. In order to achieve a minimal trauma to the inner ear structures during array insertion, it would be suitable to control insertion forces. The aim of this work was to compare the insertion forces of an array insertion into anatomical specimens with three different insertion techniques: with forceps, with a commercial tool, and with a motorized tool. Materials and Methods. Temporal bones have been mounted on a 6-axis force sensor to record insertion forces. Each temporal bone has been inserted, with a lateral wall electrode array, in random order, with each of the 3 techniques. Results. Forceps manual and commercial tool insertions generated multiple jerks during whole length insertion related to fits and starts. On the contrary, insertion force with the motorized tool only rose at the end of the insertion. Overall force momentum was 1.16 ± 0.505 N (mean ± SD, n = 10), 1.337 ± 0.408 N (n = 8), and 1.573 ± 0.764 N (n = 8) for manual insertion with forceps and commercial and motorized tools, respectively. Conclusion. Considering force momentum, no difference between the three techniques was observed. Nevertheless, a more predictable force profile could be observed with the motorized tool with a smoother rise of insertion forces.
    BioMed Research International 01/2014; 2014:532570. · 2.71 Impact Factor
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    ABSTRACT: But de la présentation Développer et pré-valider l’ECOMAS, une échelle évaluant la communication de l’adulte sourd. L’ECOMAS évalue huit domaines : « Reconnaissance de la parole », « Fluidité de l’échange », « Attitude dans la communication », « Autonomie de communication », « Intégration à des groupes sociaux », « Communication en environnement bruyant », « Communication en groupe », « Utilisation des médias ». Chaque domaine est coté de 0 à 4, soit un score total allant de 0 à 32. Matériel et méthodes Cent trente-quatre adultes sourds (76 femmes, 58 hommes) répartis en trois groupes indépendants : 39 adultes sourds candidats à l’implantation (62 ± 14 ans, [23–86]), 53 adultes implantés depuis moins d’un an (57 ± 19 ans [17–90], délai post-implant de 7 ± 4 mois, [1–12]), 42 adultes implantés depuis plus d’un an (délai post-implant de 57 ± 47 mois, [18–228]) ont été évalués avec l’ECOMAS, ainsi qu’avec la CAP, l’ERSA, les mots de Lafon/Fournier, les phrases MBAA avec un S/B de 10 dB en condition binaurale appareillée. Résultats Les scores moyens à l’ECOMAS sont respectivement de : 14 ± 7,8 sur 32 pour le groupe pré-implant (n = 39), 19 ± 6,8 pour le groupe post-implant < à 12 mois (n = 53) et 22 ± 5,5 pour le groupe post-implant > à 12 mois (n = 42). La corrélation pour chacun des trois groupes de patients entre le score total et chacun des 8 domaines (r compris entre 0,55 et 0,87, p < 0,0001), ainsi que le coefficient alpha de Cronbach de 0,9 attestent respectivement de la validité et de la consistance internes de cette échelle. La concordance inter-évaluateurs pour un même patient est très bonne entre les orthophonistes du centre référent (n = 43, coefficient Kappa > 0,8 pour les domaines, 0,68 pour le score total) mais faible entre l’orthophoniste du centre référent et l’orthophoniste de ville (n = 14, coefficient Kappa de 0,36 à 0,71 pour les domaines, 0,29 pour le score total). La validité externe entre l’Ecomas et la CAP est très bonne pour les trois groupes (r compris entre 0,69 et 0,79, p < 0,0001). Conclusion La validité interne et externe de l’ECOMAS est confirmée. Une prochaine étude aura pour objectifs d’améliorer la concordance inter-juges en développant une notice d’utilisation de l’échelle et d’évaluer la sensibilité au changement de l’échelle lors une évaluation longitudinale d’un an après l’implantation.
    Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale. 01/2014; 131(4):A12.
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    ABSTRACT: Ossicular surgery requires a high dexterity for the manipulation of the fragile and small middle ear components. Currently, the only efficient technique for training residents in otological surgery is through the use of temporal bone specimens, where any existing surgical simulator does not provide useful feedback. The objective of this study was to develop a finite-element model of the human ossicular chain dedicated to surgical simulation and to propose a method to evaluate its behavior. A model was developed based on human middle ear micromagnetic resonance imaging. The mechanical parameters were determined according to published data. To assess its performance, the middle ear transfer function was analyzed. The robustness of our model and the influence of different middle ear components were also evaluated at low frequency by static force pressure simulations. The mechanical behavior of our model in nominal and pathological conditions was in good agreement with published human temporal bone measurements. We showed that the cochlea influences the transfer function only at high frequency and could be omitted from a surgical simulator. In addition, surgeons were able to manipulate the validated middle ear model with a real-time haptic feedback. The computational efficiency of our approach allowed real-time interactions, making it suitable for use in a training simulator. © 2013 S. Karger AG, Basel.
    Audiology and Neurotology 12/2013; 19(2):73-84. · 2.32 Impact Factor
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    ABSTRACT: Most cochlear implantations are unilateral. To explore the benefits of a binaural cochlear implant, we used water-labelled oxygen-15 positron emission tomography. Relative cerebral blood flow was measured in a binaural implant group (n = 11), while the subjects were passively listening to human voice sounds, environmental sounds non-voice or silence. Binaural auditory stimulation in the cochlear implant group bilaterally activated the temporal voice areas, whereas monaural cochlear implant stimulation only activated the left temporal voice area. Direct comparison of the binaural and the monaural cochlear implant stimulation condition revealed an additional right temporal activation during voice processing in the binaural condition and the activation of a right fronto-parietal cortical network during sound processing that has been implicated in attention. These findings provide evidence that a bilateral cochlear implant stimulation enhanced the spectral cues associated with sound perception and improved brain processing of voice stimuli in the right temporal region when compared to a monaural cochlear implant stimulation. Moreover, the recruitment of sensory attention resources in a right fronto-parietal network allowed patients with bilateral cochlear implant stimulation to enhance their sound discrimination, whereas the same patients with only one cochlear implant stimulation had more auditory perception difficulties.
    Archives of Oto-Rhino-Laryngology 11/2013; · 1.29 Impact Factor
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    ABSTRACT: The aim of this study was to evaluate electrode array position in relation to cochlear anatomy and its influence on hearing performance in cochlear implantees. Twenty-two patients (25 ears) with Med-El cochlear implants were included in this retrospective study. A negative correlation was observed between electrode-modiolus distance (EMD) at the cochlear base and monosyllabic word discrimination 6 months after implantation. We found no correlation between EMD and hearing outcome at 12 months. The insertion depth/cochlear perimeter ratio appeared to negatively influence the EMD at the base. Indeed, deep insertions in small cochleae appeared to yield smaller EMD and better hearing performance. This observation supports the idea of preplanning the surgery by adapting the electrode array to the length of the available scala tympani. © 2013 S. Karger AG, Basel.
    Audiology and Neurotology 10/2013; 18(6):406-413. · 2.32 Impact Factor
  • Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale. 10/2013; 130(4):A9.
  • Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale. 10/2013; 130(4):A76.
  • Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale. 10/2013; 130(4):A76.
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    ABSTRACT: Abstract Conclusion: The use of the bone anchoring device associated with a fiducial marker, both fixed close to the operating field, improves the reproducibility and effectiveness of the computer-assisted navigation in lateral skull base surgery. Objectives: Computer-assisted navigation in lateral skull base surgery using the electromagnetic system Digipointeur® needs an external fiducial marker (titanium screw) close to the operating field to increase position accuracy (PA) to about 1 mm. Displacement of the emitter placed in the mouth (Buccostat®) induces a drift of the system, leading to at least 20% of unsuccessful procedures. The aim of this study was to evaluate the PA, stability, and reproducibility of computer-assisted navigation in lateral skull base surgery using a bone anchoring device to provide a fixed registration system near the operating field. Methods: Forty patients undergoing a lateral skull base procedure with the Digipointeur® system performed with both the titanium screw and bone anchoring device were included in this prospective study. They were divided in two groups. In the first one (n = 9), the PA was measured before and after screw registration for five intratemporal landmarks, during a translabyrinthine approach. In the second group (n = 31), all lateral skull base procedures were included and the PA was evaluated visually by the surgeon on different landmarks of the approaches as well as the stability of the system. Results: In the first group, the PA was 7.08 ± 0.59 mm and 0.77 ± 0.17 mm (mean ± SEM, p < 0.0001) before and after screw registration, respectively. In the second group, the PA was considered as accurate by the surgeon in all cases and no drift of the system was observed. Computer-assisted surgery was never abandoned due to increased stability of the bone-anchored emitter.
    Acta oto-laryngologica 08/2013; · 0.98 Impact Factor
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    ABSTRACT: The objective of this study is to evaluate the anatomical and functional results of rehabilitation of canal wall down (CWD) mastoidectomy using granules of biphasic ceramic. This is a study design retrospective in a tertiary referral centre Fifty-seven patients (59 ears) operated on between 2006 and 2010 of mastoid obliteration with granules of biphasic ceramic (TricOs(®), Maurepas, France) have been included (55 revisions and 4 first surgeries). Forty-six patients presented already a CWD mastoidectomy. The mean pre-operative bone conduction (BC) was 29 ± 3.4 dB (mean ± SEM) and mean air conduction (AC) was 57 ± 3.2 dB. Cholesteatoma was found in 33 cases. All but seven cases had post-operative otoscopy examination at 1, 3, 6 months, and 1 year postoperative with a CT scan and pure tone audiometry. Mean follow-up was 14 ± 1.8 months (3-35). At one-year follow-up (n = 52), 47 cases (90 %) presented well-healed external auditory canal. Five cases (10 %) of uncovered granules without sign of infection of external auditory canal skin were observed. Mean post-operative threshold was 25 ± 1.8 and 46 ± 1.9 dB for BC and AC , respectively (n = 47). CT scan (n = 42) showed no opacity suggesting residual disease within or behind obliteration. Mastoid obliteration with granules of biphasic ceramic is a safe and effective procedure that allows restoration of a near normal external auditory canal.
    Archives of Oto-Rhino-Laryngology 02/2013; · 1.29 Impact Factor
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    ABSTRACT: Otosclerosis is a complex disease characterized by an abnormal bone turnover of the otic capsule resulting in conductive hearing loss. Recent findings have shown that Angiotensin II (Ang II), a major effector peptide of the renin-angiotensin system, plays important role in pathophysiology of otosclerosis, most likely by its pro-inflammatory effects on the bone cells. Since reactive oxygen species play a role both in inflammation and in cellular signalling pathway of Ang II, the appearance of the "second messenger of free radicals" the aldehyde 4-hydroxynonenal (HNE) protein adducts in otosclerotic bone has been analysed. Immunohistochemical analysis of HNE-modified proteins on tissue samples of the stapedial bones performed on 15 otosclerotic patients and 6 controls, revealed regular HNE-protein adducts presence in the subperiosteal parts of control bone specimens, while irregular areas of the pronounced HNE-protein adducts presence were found within stapedial bone in case of otosclerosis. To study possible interference of HNE and Ang II in human bone cell proliferation, differentiation and induction of apoptosis we used an in vitro model of osteoblast-like cells. HNE interacted with Ang II in a dose-dependent manner, both by forming HNE-Ang II adducts, as revealed by immunoblotting, and by modification of effects on cultured cells. Namely, treatment with 0.1nM Ang II and 2.5μM HNE stimulated proliferation, while treatment with 10μM HNE or in combination with Ang II (0.1, 0.5 and 1nM) decreased cell proliferation. Moreover, 10μM HNE alone and with Ang II (except if 1nM Ang II was used) increased cellular differentiation and apoptosis. HNE 5μM did not affect differentiation or significantly changed apoptosis. On the other hand, when cells were treated with lower concentrations of HNE and Ang II we have observed decrease in cellular differentiation (combination of 1.0 or 2.5μM HNE with 0.1nM Ang II) and decrease in apoptosis (0.1 and 0.5nM Ang II). Cellular necrosis was increased with 5 and 10μM HNE if given alone or combined with Ang II, while 0.5nM Ang II and combination of 1μM HNE with Ang II (0.1 and 0.5nM) reduced necrosis. These results indicate that HNE and Ang II might act mutually dependent in regulation of the bone cell growth and in pathophysiology of otosclerosis.
    Free Radical Biology and Medicine 12/2012; · 5.27 Impact Factor
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    ABSTRACT: Abstract Conclusion: Residual hearing could be preserved with various arrays ranging from 16 to 18 mm in insertion length and 0.25 to 0.5 mm tip diameter. Whether array insertion is performed through a cochleostomy or a round window, tip diameter is an essential criterion for the array design to improve hearing preservation results. Objectives: The goal of this study was to report the outcome of patients implanted with electric acoustic cochlear implants with various surgical techniques and array designs. Methods: Thirty-two implanted ears (30 patients) were included in this retrospective study. Three array models were inserted: Contour Advance implant (n = 16), Nucleus Hybrid-L (n = 12), and Med-El Flex EAS (n = 4). Postoperative pure tone audiometry was performed at 3 and 12 months after implantation. Results: Three months postoperatively, hearing preservation within 30 dB was achieved in 50%, 50%, and 84% cases of patients implanted with a Contour Advance, Flex-EAS, and Hybrid-L, respectively. Two patients (Hybrid-L group) had a delayed sudden hearing loss (> 30 dB) 3 months postoperatively and three patients (Contour Advance group) had total hearing loss at 1 year. Best results were achieved using arrays with small tip diameters. Cochleostomy or round window insertion did not affect hearing preservation results.
    Acta oto-laryngologica 12/2012; · 0.98 Impact Factor
  • Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale. 10/2012; 129(4):A90.
  • Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale. 10/2012; 129(4):A32–A33.

Publication Stats

785 Citations
252.40 Total Impact Points

Institutions

  • 2004–2014
    • Unité Inserm U1077
      Caen, Lower Normandy, France
  • 2013
    • Hôpital La Pitié Salpêtrière (Groupe Hospitalier "La Pitié Salpêtrière - Charles Foix")
      Lutetia Parisorum, Île-de-France, France
  • 2009–2013
    • Hôpital Beaujon – Hôpitaux Universitaires Paris Nord Val de Seine
      Clicy, Île-de-France, France
  • 2003–2013
    • Paris Diderot University
      • Faculté de Médecine Xavier Bichat
      Lutetia Parisorum, Île-de-France, France
  • 2006–2012
    • Assistance Publique – Hôpitaux de Paris
      Lutetia Parisorum, Île-de-France, France
    • The University of Tokyo
      • Faculty & Graduate School of Medicine
      Tokyo, Tokyo-to, Japan
  • 2005–2009
    • The Australian Society of Otolaryngology Head & Neck Surgery
      Evans Head, New South Wales, Australia
  • 1989–2008
    • French Institute of Health and Medical Research
      Lutetia Parisorum, Île-de-France, France
  • 2007
    • Institut National de la Transfusion Sanguine, Paris
      Lutetia Parisorum, Île-de-France, France
  • 2002
    • Karolinska University Hospital
      Tukholma, Stockholm, Sweden
  • 2000
    • Karolinska Institutet
      • Institutionen för fysiologi och farmakologi
      Solna, Stockholm, Sweden
  • 1999
    • Université René Descartes - Paris 5
      Lutetia Parisorum, Île-de-France, France
  • 1998
    • University of Bordeaux
      Burdeos, Aquitaine, France
  • 1992
    • Université Victor Segalen Bordeaux 2
      Burdeos, Aquitaine, France