-
[show abstract]
[hide abstract]
ABSTRACT: Ideal biomaterials for bone tissue engineering should have capability to guide osteogenic differentiation of mesenchymal stem cells and, at the same time, to stimulate angiogenesis of endothelia cells. In this study, it was found that three Ca, Mg and Si-containing bioceramics (bredigite Ca7MgSi4O16, akermanite Ca2MgSi2O7 and diopside CaMgSi2O6) had osteogenic and angiogenic potentials. The effects of three silicate ceramics on the osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) and the angiogenesis of human aortic endothelial cells (HAECs) were explored by comparing with β-tricalcium phosphate (β-TCP) bioceramics. The proliferation, alkaline phosphatase (ALPase) activity and bone-related gene expression (COL1, ALPase, OP, BSP and OC) of hBMSCs were significantly enhanced under the stimulation of the ionic extracts of these silicate bioceramics. In addition, the results showed that extracts from the three silicate bioceramics also stimulated HAECs proliferation and in vitro angiogenesis with improved NO synthesis and angiogenic gene expression (KDR, FGFR1, ACVRL1 and NOS3). Among the three silicate ceramics, bredigite showed the highest osteogenic and angiogenic potential with the highest Si (possibly Si(OH)3O(-)) concentration in its extract, while diopside had the lowest osteogenic and angiogenic potential with the lowest Si concentration in its extract. Furthermore, it was found that the concentration of Si ion in the extracts of three silicate bioceramics was obviously higher than that of β-TCP ceramics, indicating an important role of Si ions in stimulating cell proliferation, osteogenic differentiation and angiogenesis. The results suggest that the silicate-based akermanite and bredigite ceramics might be good scaffolding biomaterials for bone tissue engineering applications due to their distinctively dual functions of osteo/angio-stimulation properties.
Acta biomaterialia 04/2013; · 3.98 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Bone cements have been widely used for orthopedic applications. Previous studies have shown that calcium silicon-based bone cements (CSC) were injectable, bioactive, biodegradable, and mechanically strong in the long term, while their short-term compressive strength was low and setting time was too long. On the other hand, plaster (CaSO(4) ·1/2H(2) O, POP) sets quickly upon contact with water and has excellent short-term compressive strength. The aim of this study is to prepare CSC/POP composite cements and investigate the effect of POP on the compressive strength, setting time, injectability, degradation, and in vitro bioactivity of the composite cements. The results have shown that POP content plays an important role to modulate the physicochemical property of CSC. The addition of POP into CSC significantly decreased the initial and final setting time and enhanced the short-term compressive strength and degradation rate. The obtained composite cement with 30% POP has been found to possess optimal setting time and short-term compressive strength. In addition, the prepared composite cements still maintain apatite-mineralization ability in simulated body fluids and their ionic extracts have no significant cytotoxicity to L929 cells. The results suggested that the addition of POP into CSC is a viable method to improve their setting properties and short-term compressive strength. The obtained composite cements with the optimized composition of 70% CSC and 30% POP could be potentially used for bone repair application. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.
Journal of Biomedical Materials Research Part B Applied Biomaterials 11/2012; · 2.15 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Hollow mesoporous silica nanoparticles (HMSNs) are considered a potential drug delivery system owing to their recognized advantages in drug loading and releasing. However, whether HMSN could be degraded inside the cells remains unknown. In this study, based on the observations by transmission electron microscopy, fluorescence staining, enzymatic proteolysis, and inductively coupled plasma atomic emission spectroscopy, HMSNs were proved to be degradable in human umbilical vein endothelial cells. The degradation first took place in cytoplasm and lysosomes, and secondly in lysosomes only. The Si content in culture medium increased as the time increases, suggesting that the degradation product inside the cells could be excreted into the culture medium. The degrading rate is fast in the first 2 days and slow after 2 days. The present results provided a clue to further research on the metabolic way and cytotoxicity of silica nanoparticles.
Journal of Biomedical Materials Research Part B Applied Biomaterials 05/2012; 100(5):1397-403. · 2.15 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: In this paper, chemically synthesized CaO-ZrO2-SiO2 (CZS) powder was plasma sprayed onto a Ti-6Al-4V alloy substrate for its potential applications in biomedicine. Surface morphology
and phase composition were analyzed by scanning electron microscopy (SEM) and x-ray diffraction (XRD). The degradation behavior
of the coating in biological environment was in vitro appraised in Tris-HCl buffer. The mass loss was much lower than that
of the wollastonite coating (WC) and close to that of the hydroxylapatite coating (HC). The bond strength with titanium alloy
substrate was 31.8±4.7MPa. In vitro bioactivity appraisement results showed that apatite could be formed on the surfaces
after soaking in simulated body fluid (SBF) for 14days. Canine marrow stem cells (MSCs) also showed well adhesion and proliferation
on the coating surfaces. In summary, results suggest that the coating possesses well cytocompatibility and may be an appropriate
candidate for application in biomedicine.
Journal of Thermal Spray Technology 04/2012; 18(4):678-685. · 1.81 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To improve the bioactivity and degradation behavior of biodegradable magnesium, biodegradable metal matrix composites with the ZK30 magnesium alloy as the matrix and bioactive glass (BG, 45S5) as the reinforcement were prepared. The microstructures of the ZK30-BG composites showed homogeneous dispersion of BG particles throughout the matrix. XRD and EDX analyses confirmed the retention of the morphological characteristics and composition of BG particles in the composites. Immersion tests in the minimum essential medium with Earle's balanced salts at 37°C showed that the composites with 5 and 10% BG had lower rates of degradation and hydrogen evolution than the matrix alloy. In addition, the tests confirmed that the composites possessed an enhanced ability to induce calcium and phosphate ion deposition on sample surfaces during degradation, suggesting accelerated surface mineralization that would lead to improved bioactivity when compared with the matrix alloy. In vitro cytotoxicity tests showed that the ionic products of the composites formed during degradation possessed a superior ability to support the survival, proliferation, and osteoblastic differentiation of bone marrow stromal cells to those of the ZK30 alloy. The ZK30-BG composites with enhanced bioactivity and reduced degradation rate could be promising biodegradable materials for orthopedic implants. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.
Journal of Biomedical Materials Research Part B Applied Biomaterials 11/2011; · 2.15 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Nanocrystalline hydroxyapatite assembled hollow fibers (NHAHF) in the membrane form were fabricated by combining the electrospinning technique and the hydrothermal method. This novel hierarchical tubular structure of hydroxyapatite exhibited excellent protein loading capacity and long-term sustained release property.
Chemical Communications 09/2011; 47(42):11674-6. · 6.17 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: The capacity to induce rapid vascular ingrowth during new bone formation is an important feature of biomaterials that are to be used for bone regeneration. Akermanite, a Ca-, Mg- and Si-containing bioceramic, has been demonstrated to be osteoinductive and to promote bone repair. This study further demonstrates the ability of akermanite to promote angiogenesis and investigates the mechanism of this behavior. The akermanite ion extract predominantly caused Si-ion-stimulated proliferation of human aortic endothelial cells. The Si ion in the extract was the most important component for the effect and the most effective concentration was found to be 0.6-2 μg ml(-1). In this range of Si ion concentration, the stimulating effect of the ceramic ion extract was demonstrated by the morphology of cells at the primary, interim and late stages during in vitro angiogenesis using ECMatrix™. The akermanite ion extract up-regulated the expression of genes encoding the receptors of proangiogenic cytokines and also increased the expression level of genes encoding the proangiogenic downstream cytokines, such as nitric oxide synthase and nitric oxide synthesis. Akermanite implanted in rabbit femoral condyle model promoted neovascularization after 8 and 16 weeks of implantation, which further confirmed its stimulation effect on angiogenesis in vivo. These results indicate that akermanite ceramic, an appropriate Si ion concentration source, could induce angiogenesis through increasing gene expression of proangiogenic cytokine receptors and up-regulated downstream signaling. To our knowledge, akermanite ceramic is the first Si-containing ceramic demonstrated to be capable of inducing angiogenesis during bone regeneration.
Acta biomaterialia 09/2011; 8(1):341-9. · 3.98 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: This study was to investigate the effects of tricalcium silicate (Ca(3)SiO(5)) on proliferation and odontogenic differentiation of human dental pulp cells (hDPCs) in vitro.
The hDPCs were seeded in culture medium with or without Ca(3)SiO(5) extract and calcium hydroxide (Ca(OH)(2)) extract. Proliferation of the hDPCs was measured by methyl-thiazol-tetrazolium (MTT) assay. Odontogenic differentiation of hDPCs was evaluated by real-time polymerase chain reaction by using odontogenic marker genes such as dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP 1), osteocalcin (OC), alkaline phosphatase (ALP), and collagen type I (Col I), which were verified by ALP activity assessment, mineralization assay, and immunocytochemistry staining for dentin sialoprotein (DSP).
The MTT assay showed that hDPCs cultured with Ca(3)SiO(5) extract proliferated more significantly as compared with Ca(OH)(2) extract. Analysis of odontogenic marker genes indicated that Ca(3)SiO(5) enhanced the expression of those genes. Moreover, the extract of Ca(3)SiO(5) stimulated mineralization and increased ALP and DSP production conspicuously.
These results reveal that Ca(3)SiO(5) can induce the proliferation and odontogenic differentiation of hDPCs in vitro and might be a potential candidate for preparation of a new type of Ca(3)SiO(5-)based cement as a pulp-capping agent.
Journal of endodontics 09/2011; 37(9):1240-6. · 2.95 Impact Factor
-
Huijie Gu,
Fangfang Guo,
Xiao Zhou,
Lunli Gong,
Yun Zhang, Wanyin Zhai,
Lei Chen,
Lian Cen,
Shuo Yin,
Jiang Chang,
Lei Cui
[show abstract]
[hide abstract]
ABSTRACT: Our previous study indicates that akermanite, a type of Ca-, Mg-, Si-containing bioceramic, can promote the osteogenic differentiation of hASCs. To elucidate the underlying mechanism, we investigated the effect of the extract from akermanite, on proliferation and osteogenic differentiation of hASCs. The original extract was obtained at 200 mg akermanite/ml LG-DMEM and further diluted with LG-DMEM. The final extracts were denoted as 1/2, 1/4, 1/8, 1/16, and 1/32 extracts based on the concentrations of the original extract. The LDH assay and live/dead stain were used to reveal the cytotoxicity of the different extracts on hASCs, while the DNA assay was carried out to quantitatively evaluate the proliferation of cells after being cultured with the extracts for 1, 3 and 7 days. Flow cytometry for cell cycle analysis was carried out on cells cultured in two media (GM and 1/2 extract) in order to further analyze the effect of the extract on cell proliferation behaviors. Osteogenic differentiation of hASCs cultured in the extracts was detected by ALP expression and calcium deposition, and further confirmed by real-time PCR analysis. It was shown that Ca, Mg and Si ions in the extract could suppress the LDH release and proliferation of hASCs, whereas promote their osteogenic differentiation. Such effects were concentration-dependent with the 1/4 extract (Ca 2.36 mM, Mg 1.11 mM, Si 1.03 mM) being the optimum in promoting the osteogenic differentiation of hASCs. An immediate increase in ERK was observed in cells cultured in the 1/4 extract and such osteogenic differentiation of hASCs promoted by released ions could be blocked by MEK1-specific inhibitor, PD98059. Briefly, Ca, Mg and Si ions extracted from akermanite in the concentrations of 2.36, 1.11, 1.03 mM, respectively, could facilitate the osteogenic differentiation of hASCs via an ERK pathway, and suppress the proliferation of hASCs without significant cytotoxicity.
Biomaterials 06/2011; 32(29):7023-33. · 7.40 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Bioactive tricalcium silicate ceramics (Ca3SiO5) were fabricated by spark plasma sintering (SPS), and their sinterability and mechanical properties were examined. The bioactivity and in vitro biocompatibility of Ca3SiO5 ceramics were evaluated. Ca3SiO5 ceramics show higher density and superior mechanical properties compared with those prepared by conventional pressureless sintering. In addition, hydroxyapatite was induced to form on the surface of Ca3SiO5 ceramics when soaked in simulated body fluid and bone marrow mesenchymal stem cells were attached and spread well on the ceramics. Ca3SiO5 ceramics fabricated by SPS possess excellent mechanical properties, bioactivity, and biocompatibility and are promising bone repaired materials.
International Journal of Applied Ceramic Technology 03/2011; 8(3):501 - 510. · 1.38 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Clinoenstatite (MgSiO3) powders were synthesized by precipitation process using magnesium nitrate (Mg(NO3)2·6H2O) and sodium silicate (Na2SiO3·9H2O), and clinoenstatite ceramics were prepared by sintering its powder compacts at 1400°C for 3 and 5 h. The shrinkage (4.53%), bending strength (31.78 MPa), relative density (82.84%), and Young's modulus (8.51 GPa) were investigated. The percentages of weight loss of the clinoenstatite ceramics were listed as 1.29±0.55%, 1.36±0.30%, 7.31±1.54%, and 10.77±0.93% after 1-, 3-, 7-, and 14-day soaking in the Tris-HCl solution. The biocompatibility studies showed that clinoenstatite ceramics could not induce the formation of HAp in simulated body fluid, but the ionic products of the clinoenstatite powder extracts promoted the proliferation of the mouse fibroblasts (L929 cell). The murine embryonic mesenchymal stem cells adhered well and proliferated more on the clinoenstatite ceramics than on the traditional hydroxyapatite ceramics. These results showed that clinoenstatite bioceramics possessed good cell biocompatibility and might be used for preparation of bone implants or biocoatings.
Journal of the American Ceramic Society 09/2010; 94(1):66 - 70. · 2.27 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: In this study, the hydroxyapatite/wollastonite (Ca10(PO4)6(OH)2/CaSiO3, HAp/CS) composite bioceramics with different weight ratio were fabricated. The effects of composite ratio on sintering behavior, microstructure, mechanical properties, bioactivity, degradability behavior and the bone marrow mesenchymal stem cells (MSC) response to the composites were investigated. When the weight ratio of CS increased, the linear shrinkage of the ceramics decreased, while the porosity increased. The bending strength of the composites could be regulated between 98.06 ± 3.27 and 221.30 ± 15.69 MPa, and increased apparently with the increase of the CS component amount. The elastic modulus of the sintered samples was about 14.88–18.95 GPa, which was similar to that of human cortical bone. The bioactivity of the composites was enhanced with increasing CS content. For composites with more than 30 wt% CS contents, the samples were completely covered by a layer of dense apatite only after 1 day soaking. The dissolution rate of the samples increased with the increase of CS content, which suggested that the degradability of the HAp/CS composite bioceramics could be tailored by adjusting the initial HAp/CS ratio. In addition, the proliferation of MSC on the composites was examined and the results showed that higher content of CS content in composites promoted cell proliferation. When the CS content in the composite increased to 30 wt%, the proliferation rate of MSC cells showed significant higher than that of pure HAp (P<0.05). Therefore, the HAp/CS composites with more than 30 wt% CS content might be promising candidates as load bearing, bioactive, and degradable biomaterials for hard tissue repair applications.
Journal of the American Ceramic Society 09/2010; 94(1):99 - 105. · 2.27 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Beta-type low elastic modulus alloys of the Ti-Nb-Zr system have recently attracted much attention for both orthopedic and dental applications. In the present study, meta-stable beta alloys of Ti-35Nb-xZr with different Zr contents were developed. The effect of Zr content on the microstructure, mechanical properties and cell attachment was investigated. It was found that the addition of Zr improved the tensile strength and elongation of Ti-35Nb-xZr alloys, and simultaneously reduced the elastic modulus. Moreover, the Zr element helped to stabilize the beta phase. Cell culture work indicated that the addition of Zr enhanced the attachment and spreading of bone marrow stem cells. Cell attachment and spreading on the surface of titanium alloys were dominated not only by the wettability but also by the inherent biocompatibility of alloying elements. The peak-aged alloy with 5 wt% Zr had a highest tensile strength of 874 MPa, while its elastic modulus was only 65 GPa, presenting a much higher strength/modulus ratio than Ti-6Al-4V. The Ti-35Nb-5Zr alloy exhibited a great potential for orthopedic and dental applications.
Biomedical Materials 08/2010; 5(4):045006. · 2.16 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Phase-pure strontium silicate (SrSiO(3)) powders were synthesized by chemical precipitation method. In vitro bioactivity of the powders were evaluated by examining the hydroxyapatite forming ability on their surface soaking simulated body fluid for various periods. The results showed that SrSiO(3) could induce hydroxy carbonate apatite formation after soaking for 7 days. Both L929 and rabbit bone marrow stromal cells (rMSCs) were used to test the in vitro cytocompatibility of SrSiO(3). L929 culture experiment showed that the ionic product of SrSiO(3) did not exhibit cytotoxicity except at high ion concentrations (Si 3.75 mM and Sr 0.12 mM). Moreover, at certain ion concentrations (Si 1.87-0.12 mM and Sr 0.12-3.75 x 10(-3) mM), the ionic product of SrSiO(3) stimulated the proliferation of rMSCs. All the results indicated that SrSiO(3) might be used as a new bioactive material for bone regeneration applications.
Journal of Biomedical Materials Research Part B Applied Biomaterials 04/2010; 93(1):252-7. · 2.15 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Willemite (Zn(2)SiO(4)) ceramics were prepared by sintering the willemite green compacts. The effects of sintering temperature on the linear shrinkage, porosity and mechanical strength of the ceramics were examined. With the sintering temperature increased, the linear shrinkage of the ceramics increased and the porosity decreased. When sintered at 1,300 degrees C, willemite ceramics showed mechanical properties of the same order of magnitude as values for human cortical bone, as measured by bending strength (91.2 +/- 4.2 MPa) and Young's modulus (37.5 +/- 1.5 GPa). In addition, the adhesion and proliferation of rabbit bone marrow stromal cells (BMSCs) on willemite ceramics was investigated. The results showed that the ceramics supported cell adhesion and stimulated the proliferation. All these findings suggest that willemite ceramics possess suitable mechanical properties and favorable biocompatibility and might be a promising biomaterial for bone implant applications.
Journal of Materials Science Materials in Medicine 04/2010; 21(4):1169-73. · 2.32 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Decellularized heart valve scaffolds possess many desirable properties in valvular tissue engineering. However, their current applications were limited by short durability, easily structural dysfunction and immunological competence. Although crosslinking with chemical reagents, such as glutaraldehyde (GA), will enhance the mechanical properties, the low long-term stability and cytotoxicity of the scaffolds remains potential problem. Nordihydroguaiaretic acid (NDGA) is a bioactive natural product which is able to crosslink collagen and was proven to be effective in preparation of scaffold for tendon tissue engineering. In this paper, NDGA crosslinked decellularized heart valve scaffolds demonstrated higher tensile strength, enzymatic hydrolysis resistance and store stability than the non-crosslinked ones. Its mechanical properties and cytocompability were superior to that of GA-crosslinked heart valve matrix. Below the concentration of 10 microg/ml, NDGA has no visible cytotoxic effect on both endothelial cells (EC) and valvular interstitial cells (VIC) and its cytotoxicity is much less than that of GA. The LC50 (50% lethal concentration) of NDGA on ECs and VICs are 32.6 microg/ml and 47.5 microg/ml, respectively, while those of GA are almost 30 times higher than NDGA (P < 0.05). ECs can attach to and maintain normal morphology on the surface of NDGA-crosslinked valvular scaffolds but not GA-crosslinked ones. This study demonstrated that NDGA-crosslinking of decellularized valvular matrix is a promising approach for preparation of heart valve tissue engineering scaffolds.
Journal of Materials Science Materials in Medicine 11/2009; 21(2):473-80. · 2.32 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Bioprosthetic heart valves, prepared by glutaraldehyde (GA) crosslinking, have some limitations due to poor durability, calcification and immunogenic reactions. The aim of this study was to evaluate the crosslinking effect of a natural product, quercetin, on decellularized porcine heart valve extracellular matrix (ECM). After crosslinking, the mechanical properties, stability, anticalcification and cytocompatibility were examined. The results showed that the tensile strength of quercetin-crosslinked ECM was higher than that of GA-crosslinked ECM. After crosslinking with quercetin, the thermal denaturation temperature of ECM was clearly increased. Quercetin-crosslinked ECM could be stored in D-Hanks solution for at least 30 days without any loss of ultimate tensile strength and elasticity. After soaking in D-Hanks solution for 36 days, there was only 11.55% non-crosslinked excess quercetin released and no further release thereafter. Cell culture study shows that no inhibition on proliferation of vascular endothelial cells occurred when the quercetin concentration was lower than 1microg ml(-1). This non-cytotoxic concentration was 100 times higher than that of GA. The resistibility of quercetin-crosslinked ECM to in vitro enzymatic hydrolysis was comparable to that of GA-crosslinked ECM. An in vitro anticalcification experiment showed that quercetin crosslinking could protect ECM from deposition of minerals in simulated body fluid. The present study demonstrated that quercetin can crosslink porcine heart valve ECM effectively, which suggests that quercetin might be a new crosslinking reagent for the preparation of bioprosthetic heart valve xenografts and scaffolds for heart valve tissue engineering.
Acta biomaterialia 08/2009; 6(2):389-95. · 3.98 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Calcification is one of the key factors for short durability of glutaraldehyde-crosslinked bioprosthetic heart valves. We have shown previously that procyanidins (PC)-crosslinked heart valve matrix has low cytotoxicity, perfect mechanical properties, and ideal stability. The aim of this study is to investigate the anticalcification effect of procyanidins and its crosslinked heart valves. Porcine aortic heart valve leaflets were decellularized and crosslinked with PC solution. The inhibition of calcification on PC-crosslinked heart valves was evaluated by soaking valves in simulated body fluid. The anticalcification effect of PC on valvular related cells was evaluated by culturing cells in the presence of PC. The results showed that minerals deposited on non- and glutaraldehyde-crosslinked valvular matrix in simulated body fluid, and PC could inhibit valve matrix mineralization in a dose-dependant manner. In addition, PC inhibited osteodifferentiation and calcification of valvular related cells by suppressing alkaline phosphatase activity and mineral deposition of cells. In conclusion, procyanidins can inhibit calcification of valvular matrix effectively by blocking mineral deposition and suppressing alkaline phosphatase activity and calcification of valvular related cells. Therefore, procyanindins-crosslinked heart valve matrix may be a potential candidate as new bioprosthetic heart valve implants.
Journal of Biomedical Materials Research Part B Applied Biomaterials 05/2009; 90(2):913-21. · 2.15 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: In this paper, chemically synthesized Mg(2)SiO(4) (MS) powder was plasma-sprayed onto a titanium alloy substrate to evaluate its application potentials in biomedicine. The phase composition and surface morphology of the MS coating were analyzed. Results showed that the MS coating was composed mainly of Mg(2)SiO(4) phase, with a small amount of MgO and glass phases. Mechanical testing showed that the coating exhibited good adhesion strength to the substrate due to the close thermal expansion coefficient between the MS ceramic and the titanium alloy substrate. The measured bonding strength was as high as 41.5+/-5.3MPa, which is much higher than the traditional HA coating. In vitro cytocompatibility evaluation of the MS coating was performed using canine bone marrow stem cells (MSCs). The MSCs exhibited good adhesion, proliferation and differentiation behavior on the MS coating surface, which can be explained by the high protein adsorption capability of the MS coating, as well as the stimulatory effects of Mg and Si ions released from the coating. The proliferation rate of the MSCs on MS coating was very close to that on the hydroxylapatite (HA) coating. Alkaline phosphatase (ALP) activity analysis demonstrated that the ALP level of the MSCs on the MS coating remained high even after 21days, implying that the surface characteristics of the coating are beneficial for the differentiation of MSCs. In summary, our results suggest that MS coating might be a new approach to prepare bone implants.
Acta biomaterialia 04/2009; 5(6):2331-7. · 3.98 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: In this study, the effects of wollastonite on proliferation and differentiation of human bone marrow-derived stromal cells (hBMSCs) have been investigated based on a polyhydroxybutyrate-co-hydroxyvalerate (PHBV)/ wollastonite (W) composite scaffolds system. Cell morphology, proliferation, and differentiation were measured. The results showed that the incorporation of wollastonite benefited hBMSCs adhesion, proliferation, and differentiation rate. In addition, an increase of proliferation and differentiation rate was observed when the wollastonite content in the PHBV/W composite scaffolds increased from 10 to 20 wt%. Based on our previous studies on PHBV/W composite discs, the differentiation measurements in this paper further proved that the wollastonite itself can stimulate the hBMSCs to differentiate toward osteoblasts without any osteogenic medium, and the ionic products (Ca and Si) released from wollastonite might contribute to this advantage. It is also suggested that the osteogenic differentiation of the hBMSCs can be affected by adjusting the wollastonite content in the composite scaffolds.
Journal of Biomaterials Applications 12/2008; 24(3):231-46. · 2.08 Impact Factor
