Jianning Wang

Publications (7)12.68 Total impact

• Article: [Diversity of C16 H33 Cl-degrading bacteria in surface seawater of the Arctic Ocean].

  Jianning Wang, Chunming Dong, Qiliang Lai, Longshan Lin, Zongze Shao
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  ABSTRACT: To detect the diversity of the degradation bacteria of halogenated-alkane form the surface seawater of the Arctic Ocean. Twelve surface-water samples from the Arctic Ocean were collected and enriched using C16 H33 Cl as the sole carbon and energy source. Bacteria from the enriched cultures were isolated on marine agar, and followed by 16S rRNA gene identification and phylogenetic analysis. Further, their degradation ability was tested with C16 H33 Cl. The bacterial community structures were further examined by denaturing gradient gel electrophoresis (DGGE). In total 112 isolates were obtained from the 12 samples, of which 19 isolates degraded C16 H33 Cl. Bacteria of Alcanivorax and Rhodococcus exerted good emulsification and degradation, whereas bacteria of Marinobacter also had the degradation capacity, but less. DGGE analysis revealed that Alcanivorax, Parvibaculum and Thioclava were dominated in the enriched consortia. The C16 H33 Cl degradation bacteria in the Arctic marine environment mainly belonged to alpha-proteobacteria, gamma-proteobacteria, actinobacteria and bacteroidetes. This is the first report on the diversity of degradation bacteria of halogenated alkane in the Arctic Ocean. Our result contributed to the knowledge about the arctic environment and the biodiversity of degrading bacteria.
  ACTA MICROBIOLOGICA SINICA 08/2012; 52(8):1011-20.
• Article: Gallaecimonas xiamenensis sp. nov., isolated from a crude oil-degrading consortium, enriched from the surface sea water around the Xiamen Island.

  Jianning Wang, Qiliang Lai, Xue Duan, Yuanyuan Fu, Liping Wang, Wanpeng Wang, Zongze Shao
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  ABSTRACT: A taxonomic study was carried out on strain 3-C-1T, which was isolated from a crude oil-degrading consortium, enriched from the surface sea water around the Xiamen Island. Cells were Gram-negative, oxidase-and catalase-positive, rod-shaped, motile by amphitrichous. Growth was observed at salinities from 0 to 12 % and at temperatures from 10 to 42°C, and it was incapable of nitrate reduction. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-C-1T belonged to the genus Gallaecimonas, with highest sequence similarity of 96.5-96.8 % to Gallaecimonas pentaromativorans CEE_131T, while the others were all below 93.0 %. The nucleotide sequence similarity of gyrB gene between strain 3-C-1T and G. pentaromativorans CEE_131T was 89.4 %. The Rep-PCR fingerprint also differentiated strain 3-C-1T from G. pentaromativorans CEE_131T. The principal fatty acids were C16:0 (21.61 %), C12:0 3OH (12.08 %), Summed Feature 3 (C16:1ω6c/ω7c) (18.12 %) and Summed Feature 8 (C18:1ω6c/ω7c) (13.22 %). The G+C content of the chromosomal DNA was 61.3 mol%. The combined genotypic and phenotypic data show that strain 3-C-1T represents a novel species of the genus Gallaecimonas, for which the name Gallaecimonas xiamenensis sp. nov. is proposed, with the type strain 3-C-1T (=CCTCC AB 209060T = LMG 25226T=MCCC 1A01354T).
  INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 06/2012; · 2.11 Impact Factor
• Article: Roseovarius indicus sp. nov., isolated from deep-sea water of the Indian Ocean.

  Qiliang Lai, Huanzi Zhong, Jianning Wang, Jun Yuan, Fengqin Sun, Liping Wang, Tianling Zheng, Zongze Shao
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  ABSTRACT: A taxonomic study was carried out on a novel bacterial strain, designated B108(T), which was isolated from a polycyclic aromatic hydrocarbon (PAH)-degrading consortium, enriched from deep-sea water of the Indian Ocean. The isolate was Gram-reaction-negative, rod-shaped and non-motile. Growth of strain B108(T) was observed in 1-15 % (w/v) NaCl and at 10-39 °C and it was unable to degrade Tween 80 or gelatin. 16S rRNA gene sequence comparisons showed that strain B108(T) was most closely related to Roseovarius halotolerans HJ50(T) (97.1 % sequence similarity), followed by Roseovarius pacificus 81-2(T) (96.6 %) and Roseovarius aestuarii SMK-122(T) (95.2 %); other species shared <95.0 % sequence similarity. DNA-DNA hybridization tests showed that strain B108(T) had a low DNA-DNA relatedness to R. halotolerans HJ50(T) and R. pacificus 81-2(T) (48±4 % and 44±5 %, respectively). The predominant fatty acids were C₁₆:₀, C₁₆:₀ 2-OH, summed feature 8 (C₁₈:₁ω7c/ω6c) and C₁₉:₀ω8c cyclo, which accounted for 84.2 % of the total cellular fatty acids. The G+C content of the chromosomal DNA was 63.6 mol%. The major respiratory quinone was ubiquinone 10 (Q10). Phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and some unidentified compounds were detected. These characteristics were in good agreement with those of members of the genus Roseovarius. The pufLM gene was also detected. According to its morphology, physiology, fatty acid composition and phylogenetic position based on 16S rRNA sequence data, the novel strain most appropriately belongs to the genus Roseovarius but can be readily distinguished from known species of this genus. Therefore, strain B108(T) represents a novel species, of the genus Roseovarius, for which the name Roseovarius indicus sp. nov. is proposed. The type strain is B108(T) ( = 2PR52-14(T)  = CCTCC AB 208233(T)  = LMG 24622(T)  = MCCC 1A01227(T)).
  INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 09/2011; 61(Pt 9):2040-4. · 2.11 Impact Factor
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  Available from: Zongze Shao

  Article: Nitratireductor pacificus sp. nov., isolated from a pyrene-degrading consortium.

  Qiliang Lai, Zhiwei Yu, Jianning Wang, Huanzi Zhong, Fengqin Sun, Liping Wang, Baojiang Wang, Zongze Shao
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  ABSTRACT: Strain pht-3B(T) was isolated from a pyrene-degrading consortium of an enriched sediment from the Pacific Ocean, collected during the screening of polycyclic aromatic hydrocarbon-degrading bacteria. Cells were Gram-negative, short rods that were motile by means of flagella. Growth was observed at 0-7 % NaCl and 10-41 °C. The isolate was able to reduce nitrate to nitrite, but not to nitrogen. 16S rRNA gene sequence comparisons showed that strain pht-3B(T) was most closely related to Nitratireductor aquibiodomus NL21(T) (97.3 % 16S rRNA gene sequence similarity), N. indicus C115(T) (97.1 %), N. basaltis J3(T) (96.8 %) and N. kimnyeongensis KY 101(T) (96.7 %). DNA-DNA hybridization between strain pht-3B(T) and these reference strains revealed 55, 54, 28 and 42 % DNA-DNA relatedness, respectively. The dominant fatty acids were C(19 : 0)ω8c cyclo (22.6 %) and summed feature 8 (consisting of C(18 : 1)ω7c and/or C(18 : 1)ω6c; 60.4 %). The G+C content of the chromosomal DNA was 63 mol%. These characteristics were in good agreement with those of members of the genus Nitratireductor. According to cell morphology, physiology, fatty acid composition, 16S rRNA gene sequence analysis and DNA-DNA relatedness, the isolate belonged to the genus Nitratireductor but could be readily distinguished from recognized species of the genus. Therefore a novel species is proposed to accommodate strain pht-3B(T), for which the name Nitratireductor pacificus sp. nov. is proposed. The type strain is pht-3B(T) ( = CCTCC AB 209302(T) = LMG 25541(T) = MCCC 1A01024(T)).
  INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 06/2011; 61(Pt 6):1386-91. · 2.11 Impact Factor
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  Available from: Zongze Shao

  Article: Alcanivorax pacificus sp. nov., isolated from a deep-sea pyrene-degrading consortium.

  Qiliang Lai, Liping Wang, Yuhui Liu, Yuanyuan Fu, Huanzi Zhong, Baojiang Wang, Liang Chen, Jianning Wang, Fengqin Sun, Zongze Shao
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  ABSTRACT: A taxonomic study was carried out on a novel bacterial strain, designated W11-5(T), which was isolated from a pyrene-degrading consortium enriched from deep-sea sediment of the Pacific Ocean. The isolate was Gram-reaction-negative and oxidase- and catalase-positive. Growth was observed in 0.5-12 % (w/v) NaCl and at 10-42 °C. On the basis of 16S rRNA gene sequence analysis, strain W11-5(T) was shown to belong to the genus Alcanivorax with a close relation to A. dieselolei B-5(T) (93.9 % 16S rRNA sequence similarity), A. balearicus MACL04(T) (93.1 %), A. hongdengensis A-11-3(T) (93.1 %), A. borkumensis SK2(T) (93.0 %), A. venustensis ISO4(T) (93.0 %) and A. jadensis T9(T) (92.9 %). Similarities between the gyrB gene sequences of W11-5(T) and other species of the genus Alcanivorax were between 76.8 and 80.8 %. The principal fatty acids were C(12 : 0) 3-OH (8.0 %), C(16 : 0) (29.1 %) and C(18 : 1)ω7c (27.4 %). The G+C content of the chromosomal DNA was 60.8 mol%. Based on its morphology, physiology and fatty acid composition as well as the results of 16S rRNA and gyrB gene sequence analyses, strain W11-5(T) ( = MCCC 1A00474(T)  = CCTCC AB 208236(T)  = LMG 25514(T)) represents a novel species of the genus Alcanivorax, for which the name Alcanivorax pacificus sp. nov. is proposed.
  INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 06/2011; 61(Pt 6):1370-4. · 2.11 Impact Factor
• Article: Idiomarina xiamenensis sp. nov., isolated from surface seawater, and proposal to transfer Pseudidiomarina aestuarii to the genus Idiomarina as Idiomarina aestuarii comb. nov.

  Liping Wang, Qiliang Lai, Yuanyuan Fu, Hua Chen, Wanpeng Wang, Jianning Wang, Fengqin Sun, Zongze Shao
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  ABSTRACT: A taxonomic study was carried out on strain 10-D-4(T), which was isolated from a crude oil-degrading consortium enriched from surface seawater collected around Xiamen Island, PR China. Strain 10-D-4(T) grew optimally at pH 7.0-8.0 and at 25 °C. The 16S rRNA gene sequence of strain 10-D-4(T) showed the highest similarity to those of Idiomarina salinarum ISL-52(T) (94.6 %), Idiomarina tainanensis PIN1(T) (94.2 %) and Idiomarina seosinensis CL-SP19(T) (94.1 %), and showed lower similarity (92.3-94.0 %) to other members of the genus Idiomarina. The major isoprenoid quinone was ubiquinone 8 (Q-8). The major fatty acids were iso-C(13 : 0) (5.2 %), iso-C(15 : 0) (15.3 %), C(16 : 0) (14.3 %), summed feature 3 (C(16 : 1)ω6c and/or C(16 : 1)ω7c) (6.6 %), iso-C(17 : 0) (15.4 %) and C(18 : 1)ω7c (13.5 %). The G+C content of the chromosomal DNA was 50.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences, together with data from phenotypic and chemotaxonomic characterization, revealed that strain 10-D-4(T) represents a novel species of the genus Idiomarina, for which the name Idiomarina xiamenensis sp. nov. is proposed. The type strain is 10-D-4(T) ( = CCTCC AB 209061(T)  = LMG 25227(T)  = MCCC 1A01370(T)). We also propose the transfer of Pseudidiomarina aestuarii, described recently, to the genus Idiomarina as Idiomarina aestuarii comb. nov. (type strain KYW314(T)  = KCTC 22740(T)  = JCM 16344(T)).
  INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 04/2011; 61(Pt 4):969-73. · 2.11 Impact Factor
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  Available from: Zongze Shao

  Article: Citreicella marina sp. nov., isolated from deep-sea sediment.

  Qiliang Lai, Yuanyuan Fu, Jianning Wang, Shuangxi Chen, Huanzi Zhong, Fengqin Sun, Zongze Shao
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  ABSTRACT: A taxonomic study was carried out on a novel strain, designated CK-I3-6(T), which was isolated from deep-sea sediment of the south-west Indian Ocean Ridge. Cells were Gram-reaction-negative, oxidase- and catalase-positive, rod-shaped and non-motile. Growth was observed at 4-38 °C and in 1-12 % (w/v) NaCl. Cells were able to degrade gelatin and oxidize thiosulfate but did not reduce nitrate. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CK-I3-6(T) belonged to the genus Citreicella with a sequence similarity of 97.3 % to Citreicella thiooxidans CHLG 1(T), while similarities with other taxa were <95.7 %. DNA-DNA hybridization showed that strain CK-I3-6(T) and C. thiooxidans CHLG 1(T) showed a low DNA-DNA relatedness (48±3 %). The principal fatty acids were C(16 : 0) (7.8 %), C(18 : 1)ω7c (66.6 %), summed feature 3 (C(16 : 1)ω6c and/or C(16 : 1)ω7c; 6.3 %) and C(19 : 0)ω8c cyclo (10.0 %). The chromosomal DNA G+C content was 67.5 mol%. On the basis of the combined genotypic and phenotypic data, strain CK-I3-6(T) represents a novel species of the genus Citreicella, for which the name Citreicella marina sp. nov. is proposed. The type strain is CK-I3-6(T) ( = CCTCC AB 209064(T)  = LMG 25230(T)  = MCCC 1A03060(T)).
  INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 04/2010; 61(Pt 4):728-31. · 2.11 Impact Factor