Guang-Hui Liu

Publications (36)277.05 Total impact

• Article: [Resetting Parkinson's disease patient-derived cells to unveil new pathological marks].

  Emmanuel Nivet, Guang-Hui Liu, Nuria Montserrat, Juan Carlos Izpisua Belmonte
  Medecine sciences: M/S 04/2013; 29(4):353-5. · 0.64 Impact Factor
• Article: Autophagic control of cell 'stemness'

  Huize Pan, Ning Cai, Mo Li, Guang-Hui Liu, Juan Carlos Izpisua Belmonte
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  ABSTRACT: Stem cells have the ability to self-renew and differentiate into various cell types. Both cell-intrinsic and extrinsic factors may contribute to aging-related decline in stem cell function and loss of stemness. The maintenance of cellular homeostasis requires timely removal of toxic proteins and damaged organelles that accumulate with age or in pathological conditions. Autophagy is one of the main strategies to eliminate unwanted cytoplasmic materials thereby ultimately preventing cellular damage. Here, we shall discuss the accumulating evidence suggesting that autophagy plays a critical role in the homeostatic control of stem cell functions during aging, tissue regeneration, and cellular reprogramming.
  EMBO Molecular Medicine 03/2013; 5(3):327-331. · 10.33 Impact Factor
• Article: Reevaluation of the safety of induced pluripotent stem cells: a call from somatic mosaicism.

  Wensu Liu, Ming Li, Jing Qu, Fei Yi, Guang-Hui Liu
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  ABSTRACT: Recent studies have been raising doubts on the safety of induced pluripotent stem cells (iPSCs) and proposing that the process of reprogramming brought about copy number variations (CNVs) in iPSCs. However, a recent paper published in Nature provided evidence showing that most CNVs were pre-existed as somatic mosaicism but not resulted from the reprogramming. This new finding would profoundly reshape some previous thoughts and endorse the confidence of iPSCs in both research and therapy.
  Protein & Cell 12/2012;
• Article: New march towards the regeneration of sensation and cognition: hear more, see more and learn more.

  Kejing Zhang, Fei Yi, Guang-Hui Liu, Juan Carlos Izpisua Belmonte
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  ABSTRACT: As many human sensory and cognitive diseases are caused by irreversible damage or loss of certain types of neurons, methodologies aimed at replacement of lost neurons are key to restore lost sensation. Recent advances in generation of ear-cell progenitors, optic-cup structures and cortical neurons from human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) provide versatile tools for modeling human diseases and developing cells for replacement therapies.
  Journal of Molecular Cell Biology 12/2012;
• Article: Beating in a dish: new hopes for cardiomyocyte regeneration.

  Ying Gu, Fei Yi, Guang-Hui Liu, Juan Carlos Izpisua Belmonte
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  ABSTRACT: Functional human cardiomyocytes hold great promise in cell transplantation-based therapy to treat many heart diseases. To meet this devastating and clinical need, researchers are infatuated with developing novel technologies and methodologies to efficiently generate cardiomyocytes through either stem cell differentiation or cell lineage transdifferentiation. Though exciting progress has been made, challenges remain to be addressed before the translation from bench side to bed side can be fulfilled.
  Cell Research 11/2012; · 8.19 Impact Factor
• Article: Progressive degeneration of human neural stem cells caused by pathogenic LRRK2.

  Guang-Hui Liu, Jing Qu, Keiichiro Suzuki, Emmanuel Nivet, Mo Li, Nuria Montserrat, Fei Yi, Xiuling Xu, Sergio Ruiz, Weiqi Zhang, [......], Ying Li, April Goebl, Jessica Kim, Rupa Devi Soligalla, Ilir Dubova, James Thompson, John Yates Iii, Concepcion Rodriguez Esteban, Ignacio Sancho-Martinez, Juan Carlos Izpisua Belmonte
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  ABSTRACT: Nuclear-architecture defects have been shown to correlate with the manifestation of a number of human diseases as well as ageing. It is therefore plausible that diseases whose manifestations correlate with ageing might be connected to the appearance of nuclear aberrations over time. We decided to evaluate nuclear organization in the context of ageing-associated disorders by focusing on a leucine-rich repeat kinase 2 (LRRK2) dominant mutation (G2019S; glycine-to-serine substitution at amino acid 2019), which is associated with familial and sporadic Parkinson's disease as well as impairment of adult neurogenesis in mice. Here we report on the generation of induced pluripotent stem cells (iPSCs) derived from Parkinson's disease patients and the implications of LRRK2(G2019S) mutation in human neural-stem-cell (NSC) populations. Mutant NSCs showed increased susceptibility to proteasomal stress as well as passage-dependent deficiencies in nuclear-envelope organization, clonal expansion and neuronal differentiation. Disease phenotypes were rescued by targeted correction of the LRRK2(G2019S) mutation with its wild-type counterpart in Parkinson's disease iPSCs and were recapitulated after targeted knock-in of the LRRK2(G2019S) mutation in human embryonic stem cells. Analysis of human brain tissue showed nuclear-envelope impairment in clinically diagnosed Parkinson's disease patients. Together, our results identify the nucleus as a previously unknown cellular organelle in Parkinson's disease pathology and may help to open new avenues for Parkinson's disease diagnoses as well as for the potential development of therapeutics targeting this fundamental cell structure.
  Nature 10/2012; · 36.28 Impact Factor
• Article: The dawn of angiogenesis modeling: regenerating vasculature from human pluripotent stem cells.

  Fei Yi, Jing Qu, Guang-Hui Liu, Juan Carlos Izpisua Belmonte
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  ABSTRACT: Efficient generation of functional human vascular endothelial cells and smooth muscle cells from pluripotent stem cells is an extensively studied topic and of great interest in the stem cell field. Though thought to be technically complex and difficult, substantial progress has been made towards this direction. Here we aim to summarize and discuss the most recent advances in this topic and their future perspective in research and clinic.
  Cell Research 09/2012; · 8.19 Impact Factor
• Article: Huntington's disease: Dancing in a dish.

  Kejing Zhang, Fei Yi, Guang-Hui Liu, Juan Carlos Izpisua Belmonte
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  ABSTRACT: In a recent landmark paper, the Huntington's disease (HD) iPSC Consortium reports on the establishment and characterization of a panel of iPSC lines from HD patients, and more importantly, the successful modeling of HD in vitro. In the same issue of Cell Stem Cell, An et al. reports on the successful targeted gene correction of HD in human iPSCs. Both advances are exciting, provide new resources for current and future HD research, and uncover new challenges to better understand and, most importantly, treat this devastating disease in the near future.
  Cell Research 08/2012; · 8.19 Impact Factor
• Article: Establishment of hepatic and neural differentiation platforms of Wilson's disease specific induced pluripotent stem cells.

  Fei Yi, Jing Qu, Mo Li, Keiichiro Suzuki, Na Young Kim, Guang-Hui Liu, Juan Carlos Izpisua Belmonte
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  ABSTRACT: The combination of disease-specific human induced pluripotent stem cells (iPSC) and directed cell differentiation offers an ideal platform for modeling and studying many inherited human diseases. Wilson's disease (WD) is a monogenic disorder of toxic copper accumulation caused by pathologic mutations of the ATP7B gene. WD affects multiple organs with primary manifestations in the liver and central nervous system (CNS). In order to better investigate the cellular pathogenesis of WD and to develop novel therapies against various WD syndromes, we sought to establish a comprehensive platform to differentiate WD patient iPSC into both hepatic and neural lineages. Here we report the generation of patient iPSC bearing a Caucasian population hotspot mutation of ATP7B. Combining with directed cell differentiation strategies, we successfully differentiated WD iPSC into hepatocyte-like cells, neural stem cells and neurons. Gene expression analysis and cDNA sequencing confirmed the expression of the mutant ATP7B gene in all differentiated cells. Hence we established a platform for studying both hepatic and neural abnormalities of WD, which may provide a new tool for tissue-specific disease modeling and drug screening in the future.
  Protein & Cell 07/2012;
• Article: Post-translational modulation of pluripotency.

  Ning Cai, Mo Li, Jing Qu, Guang-Hui Liu, Juan Carlos Izpisua Belmonte
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  ABSTRACT: The maintenance of pluripotency relies on an intricate transcriptional network hinged on a key set of transcription factors. Pluripotent stem cells have been shown to be sensitive to modulations of the cellular abundance and transcriptional activity of these key pluripotency factors. Recent evidence highlights the important role of post-translational modifications, including ubiquitination, sumoylation, phosphorylation, methylation, and acetylation, in regulating the levels and activity of pluripotency factors to achieve a balance between pluripotency and differentiation.
  Journal of Molecular Cell Biology 06/2012; 4(4):262-5.
• Article: Higher-order genomic organization in pluripotent stem cells.

  Ping Wang, Weiqi Zhang, Jiping Yang, Jing Qu, Guang-Hui Liu
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  ABSTRACT: Recent applications of new tools for genome-wide mapping of long-range and spatial interactions have shed light onto the fundamental mechanisms of three dimensional chromatin organizations in pluripotent stem cells and their derivatives.
  Protein & Cell 06/2012; 3(7):483-6.
• Article: Gametogenesis in a dish.

  Ying Gu, Guang-Hui Liu, Juan Carlos Izpisua Belmonte
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  ABSTRACT: Recent progress in the induced pluripotent stem cell (iPSC) field as well as the establishment of germline stem cell isolation and culture methodologies may provide an in vitro platform for the study of physiological and pathological human gamete development and open new avenues for cell replacement-based personalized treatment of infertility.
  Cell Research 05/2012; 22(10):1422-5. · 8.19 Impact Factor
• Article: Induced neural stem cells: a new tool for studying neural development and neurological disorders.

  Guang-Hui Liu, Fei Yi, Keiichiro Suzuki, Jing Qu, Juan Carlos Izpisua Belmonte
  Cell Research 05/2012; 22(7):1087-91. · 8.19 Impact Factor
• Article: Non-viral iPSCs: a safe way for therapy?

  Weiqi Zhang, Di Guan, Jing Qu, Weizhou Zhang, Guang-Hui Liu
  Protein & Cell 04/2012; 3(4):241-5.
• Article: Reprogramming based gene therapy for inherited red blood cell disorders.

  Xiuling Xu, Jing Qu, Keiichiro Suzuki, Mo Li, Weizhou Zhang, Guang-Hui Liu, Juan Carlos Izpisua Belmonte
  Cell Research 04/2012; 22(6):941-4. · 8.19 Impact Factor
• Article: Human induced pluripotent stem cells derived hepatocytes: rising promise for disease modeling, drug development and cell therapy.

  Fei Yi, Guang-Hui Liu, Juan Carlos Izpisua Belmonte
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  ABSTRACT: Recent advances in the study of human hepatocytes derived from induced pluripotent stem cells (iPSC) represent new promises for liver disease study and drug discovery. Human hepatocytes or hepatocyte-like cells differentiated from iPSC recapitulate many functional properties of primary human hepatocytes and have been demonstrated as a powerful and efficient tool to model human liver metabolic diseases and facilitate drug development process. In this review, we summarize the recent progress in this field and discuss the future perspective of the application of human iPSC derived hepatocytes.
  Protein & Cell 03/2012; 3(4):246-50.
• Article: Gating neural development and aging via nuclear pores.

  Guang-Hui Liu, Mo Li, Jing Qu, Juan Carlos Izpisua Belmonte
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  ABSTRACT: Emerging evidence suggests an involvement of nuclear pore components in the regulation of neural differentiation and aging. These findings will have far-ranging impacts on the understanding of the function of the nuclear envelope in physiological settings and in various neurological diseases.
  Cell Research 03/2012; 22(8):1212-4. · 8.19 Impact Factor
• Article: Converted neural cells: induced to a cure?

  Weiqi Zhang, Shunlei Duan, Ying Li, Xiuling Xu, Jing Qu, Weizhou Zhang, Guang-Hui Liu
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  ABSTRACT: Many neurodegenerative disorders such as Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS) and others often occur as a result of progressive loss of structure or function of neurons. Recently, many groups were able to generate neural cells, either differentiated from induced pluripotent stem cells (iPSCs) or converted from somatic cells. Advances in converted neural cells have opened a new era to ease applications for modeling diseases and screening drugs. In addition, the converted neural cells also hold the promise for cell replacement therapy (Kikuchi et al., 2011; Krencik et al., 2011; Kriks et al., 2011; Nori et al., 2011; Rhee et al., 2011; Schwartz et al., 2012). Here we will mainly discuss most recent progress on using converted functional neural cells to treat neurological diseases and highlight potential clinical challenges and future perspectives.
  Protein & Cell 03/2012; 3(2):91-7.
• Source

  Available from: prbb.org

  Article: Rejuvenating liver and pancreas through cell transdifferentiation.

  Fei Yi, Guang-Hui Liu, Juan Carlos Izpisua Belmonte
  Cell Research 02/2012; 22(4):616-9. · 8.19 Impact Factor
• Article: Evolution of iPSC disease models.

  Weiqi Zhang, Zhichao Ding, Guang-Hui Liu
  Protein & Cell 01/2012; 3(1):1-4.