Wenjun Wu

Rutgers, The State University of New Jersey, New Brunswick, New Jersey, United States

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Publications (55)149.26 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Periplocoside NW (PSNW) with pregnane glycoside skeleton is a novel insecticidal compound isolated from the root bark of Periploca sepium Bunge. This compound has a potent stomach poisoning activity against several insect pests. In this study, we observed the intoxication symptoms, investigated the histopathological effects and carried out immuno-electron microscopic localization of PSNW on the midgut epithelium of oriental armyworm Mythimna separata Walker larvae for better understanding its action mechanism against insects. Ultrastructural observations showed that cell damages caused by PSNW in the midgut of M. separata larvae are related to the degeneration of brush border microvilli. The dissolution of cytoskeletal structures in the interior and on the surface of microvilli was responsible for the decrease in size and eventual disappearance of microvilli when bubbles of cytoplasmic substances protrude into the midgut lumen of M. separata, thus resulting in cell death. The immuno-electron microscopic localization research showed that gold particle appeared on the microvilli layer of the midgut of M. separate larvae firstly. The density of gold particle gradually added with the time, and finally microvilli layer was destructed severely. Meantime, the gold particles were also presented to the intracellular organelle membrane and the organelles also were destructed. Therefore, we proposed that this membrane system on insect midgut epithelium cells is the initial acting site of PSNW against insects.
    Pesticide Biochemistry and Physiology 10/2014; · 2.01 Impact Factor
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    ABSTRACT: This Letter describes the on-going SAR efforts based on ML297, a potent, efficacious and selective GIRK1/2 activator (∼10-fold vs GIRK1/4 and inactive on GIRK2/3) via an iterative parallel synthesis approach. The chemical optimization at the 3-position of pyrazole within ML297 indicated that various functionalized 3-cyclopropyl moieties modulated GIRK pharmacology between inhibitor/activator within a series of 1-(3-cyclopropyl-1-phenyl-1H-pyrazol-5-yl)ureas. Importantly, novel 'molecular switches' that modulated the mode of pharmacology from inhibitor to activator was discovered on both the 3-cyclopropyl and N-phenyl moiety of the pyrazole core, providing the first highly selective GIRK1/2 activator.
    Bioorganic & Medicinal Chemistry Letters 09/2014; · 2.33 Impact Factor
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    Lina Lu, Zhijun Qi, Wenjun Wu
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    ABSTRACT: The vacuolar (H+)-ATPase (V-ATPase) of insect, which is composed of membrane-bound V0 complex and peripheral V1 complex, participates in lots of important physiological process. Subunit H, as a subunit of V1 complex, plays a vital role in bridging the communication between V1 and V0 complexes and interaction with other proteins. Yeast subunit H has been successfully crystallized through expression in E. coli, but little is known about the structure of insect subunit H. In this study, we cloned, expressed and purified the subunit H from midgut of Mythimna separata Walker. Through RACE (rapidly amplification of cDNA ends) technique, we got 1807 bp full length of subunit H, and to keep the nature structure of subunit H, we constructed Baculovirus expression vector with His-tag in the C-terminal and expressed the recombinant protein in insect sf9 cells, thereafter, purified the recombinant protein by Ni-NTA columns. Results of SDS-PAGE, western blotting and mass spectrometry showed that the recombinant protein was successfully expressed. The method of expressing and purifying M. separata subunit H will provide a foundation for obtaining the crystal of subunit H and further study of the design of novel insecticides based on its structure and function.
    International Journal of Molecular Sciences 09/2014; 15(9):15443-15455. · 2.34 Impact Factor
  • Min Lv, Wenjun Wu, Huixia Liu
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    ABSTRACT: Isolated from Dictamnus dasycarpus Turcz., fraxinellone exhibited multiple bioactivities against insects. In the present paper, the changes of digestive enzymes and detoxification enzymes of Mythimna separata Walker (5th instar larvae), treated with fraxinellone, were investigated. Compared with those of the control, the α-amylase activity of the fraxinellone-treated 5th instar larvae was inhibited, whereas the level of their protease activity was increased. Based upon further studies on the specific proteases, the levels of the active alkaline trypsin-like enzyme (BApNA as the substrate) and the chymotrypsin-like enzyme (BTEE as the substrate) activities of the treated larvae were declined; however, the level of activity of the weak alkaline trypsin-like enzyme (TAME as the substrate) of the treated ones was increased. Meanwhile, the activities of two detoxification enzymes, such as carboxylesterase (CarE) and glutathione S-transferase (GST), of the treated larvae were increased to some extent, but the activities of NADPH-P450 reductase and O-demethylase of the treated ones declined. Therefore, protease (especially the weak alkaline trypsin-like enzyme), CarE and GST played important roles in the metabolism of fraxinellone in the midgut of Mythimna separata (M. separata).
    Toxins 09/2014; 6(9):2708-2718. · 2.48 Impact Factor
    This article is viewable in ResearchGate's enriched format
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    ABSTRACT: Herein we report the discovery and SAR of an indole-based protease activated receptor-4 (PAR-4) antagonist scaffold derived from a similarity search of the Vanderbilt HTS collection, leading to MLPCN probe ML354 (VU0099704). Using a novel PAC-1 fluorescent αIIbβ3 activation assay this probe molecule antagonist was found to have an IC50 of 140nM for PAR-4 with 71-fold selectivity versus PAR-1 (PAR-1IC50=10μM).
    Bioorganic & Medicinal Chemistry Letters 08/2014; · 2.33 Impact Factor
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    ABSTRACT: In order to develop new biorational pesticides and clarify the potential structural factors needed for the biological activity of celangulin-V analogues, thirty novel nitrogenous derivatives were designed and synthesized. The single crystal structure of celangulin-V is reported for the first time and provides a more accurate structure than that previously reported. The structures of all the new derivatives were confirmed by either NMR or ESI-MSn analysis. Insecticidal activities of these compounds were tested against the third-instar larvae of Mythimna separata. One derivative (1-6) showed higher insecticidal activity than celangulin-V, with a KD50 of 231.2 microg.g(-1), while two compounds (2-13 and 2-14) exhibited lower insecticidal activities; the others revealed no activity at a concentration of 20 mg mL(-1). The results support the view that celangulin-V has the potential to be a lead structure of semi-synthetic green insecticides.
    Natural product communications 06/2014; 9(6):745-8. · 0.92 Impact Factor
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    ABSTRACT: Periplocoside NW (PSNW) is a novel insecticidal compound isolated from the root bark of Periploca sepium Bunge and has potent stomach toxicity against some insect pests. Previous studies showed that the Mythimna separata larva is sensitive to PSNW, but the Agrotis ispilon larva is insensitive. In this study, preliminary target localization on the midgut of M. separata larvae was conducted via a fluorescence labeling technique. A comparative ultrastructural study on the effects of PSNW on the midguts of M. separata and A. ispilon larvae was performed. Symptom observation results showed that typical stomach toxicity was induced by PSNW in M. separata larvae. Fluorescence localization results showed that PSNW binds to the midgut cells of M. separata larvae. Ultrastructure observations showed destruction of the microvilli, organelle, and cytomembrane in the midgut cells of M. separata larvae, whereas no obvious changes were observed in midgut cells of A. ispilon larvae. These results were consistent with the insecticidal activity of PSNW. Therefore, PSNW might act on the midgut tissues of the insects, and one or more binding sites of PSNW may exist in M. separata larvae midgut cell cytomembranes.
    Toxins 05/2014; 6(5):1575-85. · 2.48 Impact Factor
  • Chemistry of Natural Compounds 01/2014; 49(6). · 0.50 Impact Factor
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    ABSTRACT: In our continuous investigation of metabolites of Streptomyces alboflavus 313, NW-G12 (1), a novel nonchlorinated analogue of NW-G01, was obtained as a trace constituent in the weak polarity part and a known desertomycin A (2) was also isolated from the fermentation broth in the strong polarity part. The structures of compounds 1 and 2 were established by comprehensive analysis of 1D and 2D NMR spectral data. Moreover, compounds 1 and 2 showed strong antibacterial activities against Bacillus cereus, Staphylococcus aureus, and Bacillus subtilis, with MICs of less than 10 μg/mL, but both show no antibacterial activities against two gram-negative bacteria.
    Chemistry of Natural Compounds 11/2013; 49(5). · 0.50 Impact Factor
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    ABSTRACT: Go catalytic! A highly enantioselective hydrogenation of β,β-disubstituted nitroalkenes and isomeric mixtures of nitroalkenes by using a Rh/DuanPhos catalytic system under basic conditions has furnished a convenient approach to β-chiral nitroalkanes, which are otherwise not so easy to make.
    Chemistry - A European Journal 07/2013; · 5.93 Impact Factor
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    ABSTRACT: This letter describes a multi-dimensional SAR campaign based on a potent, efficacious and selective GIRK1/2 activator (∼10-fold versus GIRK1/4 and inactive on nonGIRK 1-containing GIRKs, GIRK 2 or GIRK2/3). Further chemical optimization through an iterative parallel synthesis effort identified multiple 'molecular switches' that modulated the mode of pharmacology from activator to inhibitor, as well as engendering varying selectivity profiles for GIRK1/2 and GIRK1/4. Importantly, these compounds were all inactive on nonGIRK1 containing GIRK channels. However, SAR was challenging as subtle structural modifications had large effects on both mode of pharmacology and GIRK1/2 and GIRK1/4 channel selectivity.
    Bioorganic & medicinal chemistry letters 06/2013; · 2.65 Impact Factor
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    ABSTRACT: The highly linear-selective hydroaminomethylation of styrenes is very challenging. Herein, an efficient, highly chemoselective, and linear-selective hydroaminomethylation (l/b up to >99:1) of styrenes using Rh(nbd)2SbF6 with a pyrrole-based 3,3',5,5'-substituted tetraphosphorus ligand is documented. This is in sharp contrast to other available processes leading to branched amines and provides a novel atom economic approach to 3-arylpropylamines.
    Organic Letters 06/2013; · 6.32 Impact Factor
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    ABSTRACT: In order to develop novel biorational pesticides, twelve new ether derivatives of 1β,4α,6α,9α-tetrahydroxy-2β,12-epoxymethano-β-dihydroagarofuran (3) were synthesized. The structure of the important intermediate 3was confirmed by X-ray crystallography, and the new derivatives (3.1-3.12) were elucidated by IR, 1H NMR, 13C NMR and 2D NMR spectroscopic and ESI-MS analysis. Insecticidal activities of these derivatives were tested against the third-instar larvae of Mythimna separata. Though most of the derivatives (3.1-3.4, 3.6, 3.8, 3.9-3.12) revealed no obvious activities at the concentration of 10 mg/mL, two compounds 3.5and 3.7, with KD50values of 12.9 μg/g and 7.8 μg/g, respectively, showed much higher insecticidal activities than celangulin-V, with a KD50of 321.4 μg/g, the main insecticidal component from the root bark of Chinese bittersweet, Celastrus angulatus Max. The results showed that β-dihydroagarofuran has the potential to be a lead structure for semi-synthetic green insecticides.
    Natural product communications 04/2013; 8(8):753 - 756. · 0.92 Impact Factor
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    ABSTRACT: A novel rhodium catalytic system with Naphos as ligand was developed for an efficient hydroaminomethylation of 1,1-diphenylethene under relatively mild conditions. This will allow for an atom-economic and environmentally benign synthesis of fenpiprane and related pharmaceuticals.
    Organic Letters 03/2013; 15(5):1036-9. · 6.32 Impact Factor
  • Min Lü, Wenjun Wu, Huixia Liu
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    ABSTRACT: Fraxinellone, a well-known and significant naturally occurring compound isolated from Meliaceae and Rutaceae spp. has been widely used as a drug for the treatment of tumors. On the other hand, fraxinellone exhibited a variety of insecticidal activities including feeding-deterrent activity, inhibition of growth, and larvicidal activity. The present study focused on the antifeedant and larvicidal activities of fraxinellone against the larvae of Lepidoptera, including Mythimna separata, Agrotis ypsilon, Plutella xylostella, and one kind of sanitary pest, Culux pipiens pallens. Meanwhile, the ovicidal activities and the effects of fraxinellone on the larval development of M. separata were also observed. The LC values of fraxinellone against 3rd instar larvae of M. separata, 2nd instar larvae of P. xylostella and 4th instar larvae of C. pipiens pallens were 15.95/6.43/3.60 × 10-2 mg mL-1, and its AFC values against 5th instar larvae of M. separata, 2nd instar larvae of P. xylostella and 2nd instar larvae of A. ypsilon were 10.73/7.93/12.58 mg mL-1, respectively. Compared with the control group, fraxinellone obviously inhibited the pupation rate and the growth of M. separata. Once M. separata was treated with fraxinellone at concentrations of 5.0, 10.0, and 20.0 mg mL-1, respectively, the stages from the larvae to adulthood and the egg hatching duration were prolonged to 1/2/3, and 4/3/4 days, respectively. Additionally, fraxinellone strongly inhibited the development rate and the egg hatch proportion of M. separata.
    Molecules 03/2013; 18(3):2754-62. · 2.10 Impact Factor
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    ABSTRACT: The new compound Z-4-2 was isolated from the fermentation broth of Streptomyces djakartensis NW35, together with the known compound N-acetyltryptamine (Z-9-2) by bioassay-guided fractionation. Its chemical structure was elucidated as (E)-2-methoxy-1,4 naphthoquinone-1-oxime (Z-4-2) mainly by NMR analyses and MS spectral data. Their antibacterial activities against bacteria were evaluated by the filter paper method. The results of indicated that these compounds possess significant antibacterial activities.
    Molecules 03/2013; 18(3):2763-8. · 2.10 Impact Factor
  • ChemInform 01/2013; 44(4).
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    Shaopeng Wei, Wenjun Wu, Zhiqin Ji
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    ABSTRACT: A series of (28) 1-alkyl-3-methacryloyl (acryloyl)-benzimidazolone (thione) deriv-atives were synthesized. The structures of the new derivatives were confirmed by (1)H-NMR, (13)C-NMR and ESI-MS spectral analysis. The antibacterial activities of these compounds against several strains of bacteria, such as Bacillus cereus, Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa, were evaluated by methods of paper disc-diffusion and broth mciro-dilution. Methacryloyl derivatives displayed higher antibacterial activities against tested bacterial strains than those of acryloyl derivatives in in vitro tests. A structure-activity relationship (SAR) study revealed that the presences of the methacryloyl moieties is essential to the antibacterial activities of the compounds.
    International Journal of Molecular Sciences 12/2012; 13(4):4819-30. · 2.34 Impact Factor
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    ABSTRACT: NW-G01, produced by Streptomyces alboflavus 313, is a novel cyclic hexapeptide antibiotic with many potential applications, including antimicrobial activity and antitumor agents. This study developed a system for optimizing medium components in order to enhance NW-G01 production. In this study, Plackett-Burman design (PBD) was used to find the key ingredients of medium components, and then response surface methodology (RSM) was implemented to determine their optimal concentrations. The results of PBD revealed that the crucial ingredients related to the production of NW-G01 were (NH(4))(2)SO(4), peptone and CaCO(3). A prediction model has been built in the experiments of central composite design and response surface methodology, and its validation has been further verified. The optimal medium composition was determined (g/L): corn starch 15, glucose 15, peptone 3.80, (NH(4))(2)SO(4) 0.06, NaCl 1.5, CaCO(3) 1.30, MgSO(4)·7H(2)O 0.015, K(2)HPO(4)·3H(2)O 0.015, MnCl(2)·4H(2)O 0.015, FeSO(4)·7H(2)O 0.015, and ZnSO(4)·7H(2)O 0.015. Compared with NW-G01 production (5.707 mg/L) in non-optimized fermentation medium, the production of NW-G01 (15.564 mg/L) in optimized fermentation medium had a 2.73-fold increase.
    International Journal of Molecular Sciences 12/2012; 13(4):5230-41. · 2.34 Impact Factor
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    Shaopeng Wei, Wenjun Wu, Zhiqin Ji
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    ABSTRACT: Considering the undesirable attributes of synthetic fungicides and the availability of Ficus species in China, the stem of Ficus tikoua Bur. was investigated. One new antifungal pyranoisoflavone, 5,3',4'-trihydroxy-2″,2″-dimethylpyrano (5″,6″:7,8) isoflavone (1), together with two known isoflavones, wighteone (2) and lupiwighteone (3) (with previously reported antifungal activities), were isolated from ethyl acetate extract by bioassay-guided fractionation. Their structures were determined by spectroscopic analysis, such as NMR ((1)H-(1)H COSY, HMQC, HMBC and NOESY), IR, UV and HRMS, as well as ESI-MS(n) analyses. The antifungal activities of 1-3 against Phytophthora infestans were evaluated by direct spore germination assay, and the IC(50) values were 262.442, 198.153 and 90.365 μg·mL(-1), respectively.
    International Journal of Molecular Sciences 12/2012; 13(6):7375-82. · 2.34 Impact Factor

Publication Stats

211 Citations
149.26 Total Impact Points


  • 2013
    • Rutgers, The State University of New Jersey
      • Department of Chemical Biology
      New Brunswick, New Jersey, United States
  • 2008–2013
    • Northwest A & F University
      • • College of Science
      • • Key Laboratory of Botanical Pesticides Research and Development
      • • State Key Laboratory for Crops Stress Biology in Arid Regions
      Yang-ling-chen, Shaanxi, China