Manee Luangtana-anan

Silpakorn University, Krung Thep, Bangkok, Thailand

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Publications (24)47.62 Total impact

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    ABSTRACT: The production of pharmaceutical nanoparticles by the spinning disk processing (SDP) technique has advantages in terms of its scalability and its capacity to produce readily tunable nanoparticles of narrow size distribution. In this study, we successfully developed a novel multiple stepwise SDP technique to develop aggregates of uniformly sized poly(methyl acrylates)-coated chitosan-diclofenac sodium nanocores (CS-PMA NPs) for colonic drug delivery. The processing conditions were optimized using the Box-Behnken design. SEM and TEM micrographs showed the optimized system to consist of 10 μm-sized agglomerates of CS-PMA NPs, the latter measuring 10 nm in diameter. High drug entrapment of 88% was attained. Potential colon-targeted drug release from the CS-PMA NPs was demonstrated, with retardation of drug release in simulated gastrointestinal fluids and over 90% of the drug load released into simulated colonic fluid within 8 h. Drug uptake from CS-PMA NPs into Caco-2 cells was three-fold higher than that from a control drug solution, with no apparent cytotoxicity observed at the NP doses administered. The collective data suggest that the SDP is a robust manufacturing method that can potentially be used to scale up the production of composite nanoparticulate colon-targeted drug delivery systems.
    European journal of pharmaceutical sciences: official journal of the European Federation for Pharmaceutical Sciences 07/2013; · 2.61 Impact Factor
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    ABSTRACT: The objective of the present study was to reduce the polymerization of shellac by the formation of composite films with gelatin. The 6% w/w of composite film based on shellac and gelatin was prepared by the incorporation of different concentrations of gelatin and was prepared by film casting method. The stability of the composite films was then studied at 40 °C, 75% RH for 180 days in a stability chamber and the physicochemical properties were investigated. The results demonstrated that the higher concentrations of gelatin (30%, 40% and 50%) contributed to slight change in the acid value and the percentage of insoluble solids upon 180 days of storage. These results were due to the protection at the active sites of the shellac, carboxyl and hydroxyl groups, by the electrostatic interaction between the negative charge of shellac and the positive charge of gelatin, resulting in the stabilized shellac. However, the mechanical properties could not withstand the storage time; further investigation was required to improve the stability in terms of mechanical properties of the composite film by the addition of a plasticizer. Two types of plasticizers were used: polyethylene glycol (PEG) and diethyl phthalate (DEP) represented hydrophilic and hydrophobic plasticizers at the concentrations of 5% and 10%, respectively. The addition of both types and concentrations of plasticizers could improve the poor mechanical properties and brittleness of the composite film for a longer storage time. However, only PEG 400 at 5% and 10% w/w acted as a good plasticizer capable of improving the stability of the composite film under the longer periods of storage. Therefore, the attempt to improve the stability in terms of polymerization and mechanical properties of shellac by the formation of the composite film and the addition of the plasticizer could be achieved. The result could be used to develop a new material for the various applications of edible films for coating in food and pharmaceutical industries.
    Journal of Food Engineering. 05/2013; 116(2):572–580.
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    ABSTRACT: The potential of using two natural polymers (chitosan and shellac) for the formation of nanoparticles by the process of ionic cross-linking to encapsulate bovine serum albumin, a model protein was investigated. Depending on the concentrations of chitosan, shellac and bovine serum albumin, three physical states - nanoparticle, aggregation, and solution could be observed as a result of the electrostatic force. The formation of nanoparticles was due to the balance between the repulsion force and attractive force while the imbalance between both forces resulted in the formation of aggregation and solution. The Fourier transform infrared spectroscopy and differential scanning calorimetry were applied to prove the nanoparticle formation. The particle size was characterized by the light scattering technique and was found in the range between 100 and 300 nm. The morphology of the particles, detected by transmission electron microscopy was spherical shape. The result showed that the zeta potential of the nanoparticles possessed positive charges. The concentrations of chitosan, shellac and bovine serum albumin had an influence on the physicochemical properties of the nanoparticles such as the particle size, the zeta potential, the encapsulation, the loading efficiencies and the cumulative release. Therefore, chitosan and shellac could be used to form nanoparticles for protein delivery by the ionic cross-linking method.
    Pharmaceutical Development and Technology 05/2012; · 1.33 Impact Factor
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    ABSTRACT: Magnetic resonance imaging (MRI) was used to assess in situ swelling behaviors of spray-dried chitosan acetate (CSA) in 0.1N HCl, pH 6.8 and pH 5.0 Tris-HCl buffers. The in vitro drug releases from CSA matrix tablets containing the model drugs, diclofenac sodium and theophylline were investigated in all media using USP-4 apparatus. The effect of chitosan molecular weight, especially in pH 6.8 Tris-HCl, was also studied. In 0.1N HCl, the drug release from the matrix tablets was the lowest in relation to the highest swelling of CSA. The swelling kinetics in Tris-HCl buffers are Fickian diffusion according to their best fit to Higuchi's model as well as the drug release kinetics in all the media. The high swelling rate (k(s)(')) was found to delay the drug release rate (k'). The linear relationship between the swelling and fractions of drug release in Tris-HCl buffers was observed, indicating an important role of the swelling on controlling the drug release mechanism. Additionally, CSA of 200 and 800 kDa chitosan did not swell in pH 6.8 Tris-HCl but disintegrated into fractions, and the drug release from the matrix tablets was the highest.
    European journal of pharmaceutics and biopharmaceutics: official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 02/2011; 77(2):320-6. · 3.15 Impact Factor
  • The 4th Silpakorn University Research Fair; 01/2011
  • Pure and Applied Chemistry International Conference (PACCON); 01/2011
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    Manee Luangtana-anan, Jurairat Nunthanid, Sontaya Limmatvapirat
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    ABSTRACT: The effects of molecular weight and concentration of plasticizer on physicochemical properties and stability of shellac films were investigated. Type of plasticizer was previously reported to have some effects on the stability of shellac films, and polyethylene glycol (PEG) was the plasticizer of choice for plasticizing shellac films. In this study, different molecular weights of PEG (200, 400 and 4000) were chosen at a concentration of 10% w/w of shellac films. Shellac in alcohol was prepared in a free film. The stability of shellac film was then performed at 75% RH, 40 °C for 3 months. The comparison was made between the film with and without plasticizer. Shellac films were then determined for acid value, insoluble solid, mechanical properties and water vapor permeability coefficient. It was reported that different molecular weights of PEG had some influence on physicochemical properties of the shellac films. Among different molecular weights of PEG, PEG 400 showed a suitable molecular weight that could protect the shellac chain at the carboxylic and hydroxyl groups. Therefore, the molecular weight of plasticizer played a crucial role for the protective ability at active sites. Further study was performed to investigate the effect of concentrations of PEG 400 on the stability. The results demonstrated that PEG 400 at a concentration of 10% (w/w) could prevent the polymerization process for only 4 months and a significant change of all parameters was then reported. However, a higher concentration, 20% (w/w) of PEG 400, could prolong the stability of shellac for 6 months of study. Therefore, the drawback of shellac as a natural polymer in pharmaceutical and food industries could be tackled by the appropriate size and concentration of plasticizer.
    Journal of Agricultural and Food Chemistry 12/2010; 58(24):12934-40. · 3.11 Impact Factor
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    ABSTRACT: Stability enhancement of protein-loaded chitosan microparticles under storage was investigated. Chitosan glutamate at 35 kDa and bovine serum albumin as model protein drug were used in this study. The chitosan microparticles were prepared by ionotropic gelation, and polyethylene glycol 200 (PEG 200) was applied after the formation of the particles. All chitosan microparticles were kept at 25°C for 28 days. A comparison was made between those preparations with PEG 200 and without PEG 200. The changes in the physicochemical properties of the microparticles such as size, zeta potential, pH, and percent loading capacity were investigated after 0, 3, 7, 14, and 28 days of storage. It was found that the stability decreased upon storage and the aggregation of microparticles could be observed for both preparations. The reduction in the zeta potential and the increase in the pH, size, and loading capacity were observed when they were kept at a longer period. The significant change of those preparations without PEG 200 was evident after 7 days of storage whereas those with PEG 200 underwent smaller changes with enhanced stability after 28 days of storage. Therefore, this investigation gave valuable information on the stability enhancement of the microparticles. Hence, enhanced stability of chitosan glutamate microparticles for the delivery of protein could be achieved by the application of PEG 200.
    AAPS PharmSciTech 09/2010; 11(3):1376-82. · 1.58 Impact Factor
  • The 6th Thailand Materials Science and Technology Conference (MSAT-6); 08/2010
  • The 6th Thailand Materials Science and Technology Conference (MSAT-6); 08/2010
  • The 7th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology; 03/2010
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    ABSTRACT: Pellets containing microcrystalline cellulose (MCC), a model drug (theophylline) and a range of levels of sodium alginate (i.e., 10-50% w/w) were prepared by extrusion/spheronization. Two types of sodium alginate were evaluated with and without the addition of either calcium acetate or calcium carbonate (0, 0.3, 3 and 10% w/w). The effects of amount and type of sodium alginate and calcium salts on pellet properties, e.g., size, shape, morphology and drug release behavior, were investigated. Most pellet formulations resulted in pellets of a sufficient quality with respect to size, size distribution and shape. The results showed that the amounts of sodium alginate and calcium salts influenced the size and shape of the obtained pellets. However, different types of sodium alginate and calcium salt responded to modifications to a different extent. A cavity was observed in the pellet structure, as seen in the scanning electron micrographs, resulting from the forces involved in the spheronization process. Most of pellet formulations released about 75-85% drug within 60 min. Incorporation of calcium salts in the pellet formulations altered the drug release, depending on the solubility of the calcium salts used. The drug release data showed a good fit into both Higuchi and Korsmeyer-Peppas equations.
    European Journal of Pharmaceutics and Biopharmaceutics 06/2008; 69(1):274-84. · 3.83 Impact Factor
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    ABSTRACT: The aim of this study was to improve enteric properties of shellac by the formation of succinate derivative through dry media reaction. Shellac and succinic anhydride were mixed and then co-ground by planetary ball mill. The ground mixture was then activated by heating for various times and washed for removal of excess succinic anhydride. The ground mixtures and the heat-activated mixtures were characterized by physical and chemical tests, including acid value, FTIR spectroscopy, (1)H NMR and (13)C NMR spectroscopy, thermal analysis and film properties. The results demonstrated that acid values of heat-activated shellac mixtures increased with the increase of annealing time, suggesting the presence of carboxylic acid moieties of succinate at shellac molecules. The results were in good agreement with the DSC thermograms. The melting peak of shellac disappeared after heating, while melting peak of succinic anhydride gradually decreased, suggesting the utilization of succinic anhydride for the esterification. The shellac succinate formation was also confirmed by (1)H NMR and (13)C NMR spectroscopies. Film prepared from shellac succinate showed improved solubility, especially at the pH of small intestine (5.8-6.7), as compared to native shellac. The shellac succinate film also demonstrated better mechanical property, in terms of increased flexibility. In conclusion, solid-state formation of shellac succinate ester, which had improved enteric properties, was easily accomplished under the concept of "green approach".
    European Journal of Pharmaceutics and Biopharmaceutics 04/2008; 70(1):335-44. · 3.83 Impact Factor
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    ABSTRACT: A new oral-controlled release matrix tablet based on shellac polymer was designed and developed, using metronidazole (MZ) as a model drug. The shellac-based matrix tablets were prepared by wet granulation using different amounts of shellac and lactose. The effect of annealing temperature and pH of medium on drug release from matrix tablets was investigated. The increased amount of shellac and increased annealing temperature significantly affected the physical properties (i.e., tablet hardness and tablet disintegration) and MZ release from the matrix tablets. The in-situ polymerization played a major role on the changes in shellac properties during annealing process. Though the shellac did not dissolve in acid medium, the MZ release in 0.1N HCl was faster than in pH 7.3 buffer, resulting from a higher solubility of MZ in acid medium. The modulation of MZ release kinetics from shellac-based matrix tablets could be accomplished by varying the amount of shellac or annealing temperature. The release kinetics was shifted from relaxation-controlled release to diffusion-controlled release when the amount of shellac or the annealing temperature was increased.
    European Journal of Pharmaceutics and Biopharmaceutics 03/2008; 69(3):1004-13. · 3.83 Impact Factor
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    ABSTRACT: A colonic drug delivery with a new concept based on a combination of time-, pH-, and enzyme-controlled system was developed. Spray-dried chitosan acetate (CSA) prepared from low molecular weight chitosan was characterized. A combination of CSA and hydroxypropyl methylcellulose (HPMC) was used as new compression-coats for 5-aminosalicylic acid (5-ASA) tablets. Factors affecting in-vitro drug release, i.e. % weight ratio of coating polymers, enzyme activity, pH of media, and excipients in core tablets, were evaluated. The tablets compression-coated with HPMC:CSA at 60:40 and 50:50% weight ratio providing lag times about 5-6h were able to pass through the stomach (stage I, 0.1N HCl) and small intestine (stage II, pH 6.8, Tris-HCl). The delayed release was time- and pH-controlled owing to the swelling with gradual dissolving of CSA and HPMC in 0.1N HCl and the less solubility of CSA at higher pH. After reaching the colon (stage III, pH 5.0, acetate buffer), the dissolution of CSA at low pH triggered the drug release over 90% within 14h. Furthermore, the degradation of CSA by beta-glucosidase in the colonic fluid enhanced the drug release while adding the disintegrant or the osmotic agent in the core tablets would affect the drug release.
    European Journal of Pharmaceutics and Biopharmaceutics 03/2008; 68(2):253-9. · 3.83 Impact Factor
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    ABSTRACT: The purpose of this study was to prepare wax-incorporated pectin-based emulsion gel beads using a modified emulsion-gelation method. The waxes in pectin-olive oil mixtures containing a model drug, metronidazole, were hot-melted, homogenized and then extruded into calcium chloride solution. The beads formed were separated, washed with distilled water and dried for 12 h. The influence of various types and amounts of wax on floating and drug release behavior of emulsion gel beads of calcium pectinate was investigated. The drug-loaded gel beads were found to float on simulated gastric fluid if the sufficient amount of oil was used. Incorporation of wax into the emulsion gel beads affected the drug release. Water-soluble wax (i.e. polyethylene glycol) increased the drug release while other water-insoluble waxes (i.e. glyceryl monostearate, stearyl alcohol, carnauba wax, spermaceti wax and white wax) significantly retarded the drug release. Different waxes had a slight effect on the drug release. However, the increased amount of incorporated wax in the formulations significantly sustained the drug release while the beads remained floating. The results suggest that wax-incorporated emulsion gel beads could be used as a carrier for intragastric floating drug delivery.
    AAPS PharmSciTech 02/2008; 9(2):571-6. · 1.58 Impact Factor
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    ABSTRACT: This work aimed at studying factors affecting preparations of chitosan microcapsules for colonic drug delivery. Chitosan microcores (CS, 45kDa, 87% degree of deacetylation) containing diclofenac sodium (DS) coated with Eudragit®S100 (ED) were prepared by a desolvation technique. Sodium sulfate was used as a desolvating agent and the drying process was freeze-drying. Factors affecting morphology, particle size and zeta potential of microcapsules were evaluated, i.e. weight ratio of DS:CS:ED, surfactant (polysorbate 80), anti-adherent (silicon dioxide), and the use of sonication or homogenization in preparation processes. The weight ratio of DS:CS:ED at 1:2:6 provided the smallest microcapsules of about 82.37±1.61 micrometer in diameter and they were in aggregated forms. Zeta potential of the microcapsules was around -25.74 +4.78 mV which indicated that the core particles of CS and DS with zeta potential of 42.14±1.74 mV were encapsulated by ED. Increasing the amount of CS and ED, the size of microcapsules was increased but the zeta potential was not affected. Adding of polysorbate 80 could not reduce the size of microcapsules, but silicon dioxide could reduce the size and aggregation of microcapsules. Finally, the use of sonication and homogenization were effective in reducing of the size of microcapsules to 53.45 +0.63 and 58.72+1.28 micrometers, respectively.
    01/2008;
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    ABSTRACT: The objective of this study was to improve the properties of shellac by composite salts formation. The shellac samples were prepared in various salt forms by dissolving them with 2-amino-2-methyl-1-propanol (AMP) and ammonium hydroxide (AMN) at various ratios of AMP:AMN. The results demonstrated that aqueous solubility of the shellac salts was improved as the ratio of AMP:AMN increased. The absorbance ratio of the FTIR peaks assigned to CO stretching of carboxylate and carboxylic acid (ABS1556/ABS1716) was increased with the increase of the AMP fraction, suggesting that the solubility enhancement was due to more ionization of AMP salts. Moisture adsorption studies indicated that shellac salts were more hygroscopic as AMP content increased. After storage at 40 degrees C, 75% RH, the acid value and insoluble solid of AMP salts were relatively constant even after storage of up to 180 days, suggesting that AMP should protect polymerization. The ABS1556/ABS1716 values of the shellac salts were rapidly decreased after storage, especially for those consisting of a high percentage of AMN. Thus, AMP should bind much tighter at the carboxylate binding site as compared with AMN, resulting in more solubility and stability. In conclusion, optimized shellac properties could be easily accomplished by composite salts formation.
    European Journal of Pharmaceutics and Biopharmaceutics 12/2007; 67(3):690-8. · 3.83 Impact Factor
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    ABSTRACT: The aim of this study was to investigate the possibility of producing alginate-based pellets by extrusion/spheronization and also to improve the formation of spherical alginate-based pellets by investigating the effect of additive in granulating liquid on characteristics and drug release from resulting pellets. Two types of sodium alginate (30%) were evaluated in combination with theophylline (20%), microcrystalline cellulose (50%) and different granulation liquids. The pellets were then prepared in a basket extruder, then spheronized and dried. The final products were characterized by morphological examination and drug release study. Different additives in the granulating liquid influenced the ability of the extruded mass to form pellets (the processability) with this technique. However, different sodium alginate types responded to shape modifications to a different extent. Long, dumbbell-shaped pellets were obtained with viscous granulating liquids. However, short, nearly spherical pellets were obtained with watery granulation liquid with calcium chloride that reduced the swelling ability of sodium alginate. Improvements in the pellet characteristics were also dependent on the sodium alginate type employed. Most of pellet formulations released about 75-85% drug within 60min and showed a good fit into both Higuchi and Korsmeyer-Peppas equations. Higher amount of 3% calcium chloride, as a granulating liquid, in the formulation showed higher mean dissolution time resulting from the cross-linking properties of calcium ions to the negative charges of alginate molecules.
    European Journal of Pharmaceutics and Biopharmaceutics 09/2007; 67(1):227-35. · 3.83 Impact Factor
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    ABSTRACT: The aim of this study was to increase the stability of shellac because of the polymerization. A few approaches have been applied in this study. Shellac film was prepared in two salt forms, that is, ammonium and 2-methyl-2-amino 1-propanol salts, and a comparison was made with shellac film in free acid form. The other approach was by the application of plasticizers. These plasticizers were diethyl phathalate, triacetin, and polyethylene glycol 400 (PEG 400). Plasticized shellac and unplasticized shellac films in free acid form were then compared. All shellac films were kept in stability chamber at 40 degrees C, 75% RH for a period of 3 months. The studied parameters such as insoluble solid, acid value, mechanical properties, and water vapor permeability were detected every month. Analysis of variance (ANOVA) technique was used to analyze data. The applications of salt forms proved statistically significant (p < 0.01) to reduce the polymerization process whereas certain plasticizers could enhance the stability. PEG 400 was the only plasticizer that could show the increase in stability. The improvement of stability might be a result of the interference of a larger molecule of PEG 400 causing the difficulty in interaction among carboxyl or hydroxyl groups of shellac and the effect of lower loss of plasticizer.
    Journal of Agricultural and Food Chemistry 02/2007; 55(3):687-92. · 3.11 Impact Factor