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M K Ju,
K H Huh, K T Park,
S J Kim,
B H Cho,
C D Kim,
B J So,
C M Kang,
S Lee,
D J Joo, Y S Kim
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ABSTRACT: The present study compared the efficacy and safety of mizoribine (MZR) with mycophenolate mofetil (MMF) in kidney transplantation. This multicenter, randomized clinical trial. Employed doses of study drug tailored to the immunosuppressive need. The primary efficacy outcome was the incidence of biopsy-proven acute rejection episodes (BPAR). The safety of the study drug was assessed using the incidences of adverse events, drug discontinuations, and abnormal laboratory results. The 7 (6.4%) BPARs above grade II were observed in the MZR group noninferior to the 2 (1.8%) in the MMF group (95% confidence interval, -0.007-0.097 > noninferiority limit [-0.2]). BPAR was significantly decreased in the MZR group after the dose change (17/41 [41.4%] vs 8/69 [11.6%]; P < .0001) and the incidence of BPAR was similar between the MZR and MMF groups after the dose change (P = .592). The uric acid level was significantly elevated in the MZR group (P = .002). In conclusion, the efficacy and safety of MZR were similar and statistically noninferior to MMF in combination therapy with tacrolimus.
Transplantation Proceedings 05/2013; 45(4):1481-1486. · 1.00 Impact Factor
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E.J. Van Nieukerken,
L. Kaila,
I.J. Kitching,
N.P. Kristensen,
D.C. Lees,
J. Minet,
C. Mitter,
M. Mutanen,
J.C. Regier,
T.J. Simonsen, [......],
A. Schintlmeister,
B.C. Schmidt,
J.-C. Sohn,
M.A. Solis,
G.M. Tarmann,
A.D. Warren,
S. Weller,
R.V. Yakovlev,
V.V. Zolotuhin,
A. Zwick
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ABSTRACT: “Order Lepidoptera Linnaeus, 1758. In: Zhang, Z.-Q. (Ed.) Animal biodiversity: An outline of higher-level classification and survey of taxonomic richness”.
Zootaxa 01/2011; 3148:212-221. · 0.93 Impact Factor
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ABSTRACT: We report the first case of parainfluenza virus type 3 (PIV3) pneumonia in a kidney transplant recipient. A 39-year-old man developed pneumonia during hospitalization 6 years after kidney transplantation. He became hypoxic and underwent noninvasive ventilation. PIV3 was detected in the bronchoalveolar lavage fluid. He was treated successfully with aerosolized ribavirin and intravenous immunoglobulin. Although he recovered from pneumonia, his graft function deteriorated and he had to restart peritoneal dialysis.
Transplant Infectious Disease 07/2009; 11(4):333 - 336. · 2.22 Impact Factor
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ABSTRACT: We report the first case of parainfluenza virus type 3 (PIV3) pneumonia in a kidney transplant recipient. A 39-year-old man developed pneumonia during hospitalization 6 years after kidney transplantation. He became hypoxic and underwent noninvasive ventilation. PIV3 was detected in the bronchoalveolar lavage fluid. He was treated successfully with aerosolized ribavirin and intravenous immunoglobulin. Although he recovered from pneumonia, his graft function deteriorated and he had to restart peritoneal dialysis.
Transplant Infectious Disease 05/2009; 11(4):333-6. · 2.22 Impact Factor
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ABSTRACT: Transplantation of microencapsulated islets is proposed as an ideal therapy for the treatment of type 1 diabetes mellitus without immunosuppression. This strategy is based on the principle that foreign cells are protected from the host immune system by an artificial membrane. The aim of this study was to establish an ideal condition of microencapsulation using an air-driven droplet generator and alginate in vitro. The optimal conditions for islet encapsulation were an alginate inflow rate of 10 mL/h, CO2 flow rate of 2.0 L/min in a concentration of 2% alginate. For 2.5% alginate, the alginate inflow rate of 20 mL/h, CO2 flow rate 3.0 L/min was ideal; alginate inflow rate of 40 mL/h, CO2 flow rate of 4.0 L/min showed good microcapsules at 3% alginate. Viability of encapsulated islets was greater than 90%. In terms of insulin secretion, encapsulated islets secreted insulin in response to glucose in static culture medium. However, there was no normal response to low or high glucose challenge with a stimulation index less than 2.0. Microencapsulation of pig islets was successfully performed with air-driven droplet generator and alginate in vitro. Further studies about biocompatibility and glucose control in vivo may provide a useful tool for treatment of patients with diabetes mellitus.
Transplantation Proceedings 11/2008; 40(8):2578-80. · 1.00 Impact Factor
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ABSTRACT: Adult porcine islet xenotransplantation into humans is greatly diminished by the difficulty to isolate islets because of their fragility. The goal of this study was to improve the efficacy of islet yields using endogenous trypsin inhibitor and histidine-tryptophan-ketoglutarate (HTK) perfusate.
We compared two porcine islet isolation protocols: Eurocollins solution for in situ pancreas perfusion without use of an endogenous trypsin inhibitor versus HTK solution including endogenous trypsin inhibitor for pancreas perfusion.
Endogenous trypsin inhibitor and HTK strategies significantly improved total islet yield, recovery, and islet index after purification (P < .05), whereas unpurified islet yield did not increase. An average of 228,000 +/- 95,000 islet equivalents (IEQ) (n = 20) purified islets were obtained in the first group compared with 115,000 +/- 56,000 IEQ (n = 18) in the second group. The average islet index was significantly increased in the first group compared with the second group before and after purification: before: 0.28 versus 0.49 versus after: 0.25 versus 0.4 (P < .05). At this time, islet purity, viability, and stimulation index did not show a significant difference between groups.
Our study showed that endogenous trypsin inhibitor and HTK strategies significantly improved purified islet isolation efficacy because of reduction of islet fragility.
Transplantation Proceedings 10/2008; 40(8):2585-7. · 1.00 Impact Factor
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ABSTRACT: Botulinum toxin type A (BTX-A) reduces the muscular contractions by temporarily inhibiting the release of acetylcholine at the neuromuscular junction. The purpose of this study was to investigate the effects of the BTX-A injected into the masseter muscle of a developing rat mandible.
Four-week-old male (no. 80) Sprague-Dawley rats were divided into four groups: control group, saline group, BTX-A group and baseline control group. Rats of baseline group were sacrificed at 0 day to provide baseline values of the mandibular measurements. The masseter muscle of rats in the saline and the BTX-A group were administered with saline and BTX-A solutions respectively. Experimental animals were sacrificed after 4 weeks.
The BTX-A group demonstrated smaller mandibular dimension compared with the other groups (P < 0.05). Their condylar cartilages showed increased apoptosis at the proliferation stage of the reserve zone and masseter muscle fibers demonstrated atrophic changes.
The result demonstrated BTX-A influence on inhibitory action of the developing mandible because of apoptosis at the proliferation stage of the reserve zone of the condylar cartilage in developing rat mandible.
Oral Diseases 04/2008; 14(7):626-32. · 2.49 Impact Factor
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N H Kwon,
S H Kim, K T Park,
W K Bae,
J Y Kim,
J Y Lim,
J S Ahn,
K S Lyoo,
J M Kim,
W K Jung,
K M Noh,
G A Bohach,
Y H Park
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ABSTRACT: The extended single-reaction multiplex PCR (esr-mPCR) developed in this study to detect staphylococcal enterotoxins (SEs), including SEA, SEB, SEC, SED, SEE, SEH, SEI, and SEJ, requires fewer sets of primers than other conventional multiplex PCRs and can be used to detect newly identified staphylococcal enterotoxins SEs more readily. Esr-mPCR analysis of 141 isolates of Staphylococcus aureus obtained from abattoir and livestock product samples revealed that 27 of the S. aureus isolates were toxigenic, and two were 2 multitoxigenic isolates. The most prevalent SE type was SEI followed by SEA and SEH. In addition, we investigated the clonal relatedness of toxigenic S. aureus isolates by arbitrarily primed PCR (AP-PCR). AP-PCR analysis of toxigenic S. aureus isolates revealed that the discriminatory power of AP-PCR was 9 (D=0.81), 8 (D=0.77), and 10 types (D=0.83) with primers AP1, ERIC2, and AP7, respectively. The combination of three each AP-PCR result could rearrange toxigenic S. aureus isolates into 10 types and five subtypes, with the D-value of 0.92. Interestingly, our data showed that toxigenic S. aureus isolates from different sources had different fingerprinting patterns although some of them carried the same types of SE genes. These data suggest that combinations of esr-mPCR and AP-PCR can provide a powerful approach for epidemiological investigation of toxigenic S. aureus isolates.
International Journal of Food Microbiology 01/2005; 97(2):137-45. · 3.33 Impact Factor
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N H Kwon,
S H Kim,
J Y Kim,
J Y Lim,
J M Kim,
W K Jung, K T Park,
W K Bae,
K M Noh,
J W Choi,
J Hur,
Y H Park
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ABSTRACT: An efficacy test of GC-100X, a noncorrosive alkaline ionic fluid (pH 12) composed of free radicals and supplemented with xylitol, was carried out against six major foodborne pathogens-Staphylococcus aureus FRI 913, Salmonella enterica serovar Enteritidis ATCC 13076, S. enterica serovar Typhimurium DT104 Korean isolate, Vibrio parahaemolyticus ATCC 17803, Escherichia coli O157:H7 ATCC 43894, and Pseudomonas aeruginosa KCTC 1637-at three different temperatures (4, 25, and 36 degrees C) with or without organic load (2% yeast extract). Results revealed a more than 4-log10 (CFU/ml) reduction (1.0 x 10(4) CFU/ml reduction) against all pathogens reacted at 37 degrees C for 3 h in the absence of organic material. GC-100X solution diluted with an equal volume of distilled or standard hard water (300 ppm CaCO3) showed effective bactericidal activity, particularly against gram-negative bacteria. Washing efficacy of GC-100X solution was compared against E. coli O157:H7 on cherry tomato surfaces with those of a commercially used detergent and chlorine water (100 ppm). Viable cell counts of E. coli O157:H7 that had penetrated to the cores of tomatoes after sanitizing treatment revealed that GC-100X stock and its 5% diluted solutions had similar washing effects to 100-ppm chlorine water and were more effective than the other kitchen detergent. These results indicate that GC-100X has good bactericidal and sanitizing activities and is useful as a new sanitizer for food safety and kitchen hygiene.
Journal of food protection 10/2003; 66(9):1604-10. · 1.94 Impact Factor
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ABSTRACT: To evaluate the usefulness of power spectral analysis on fetal heart rate variability as a new diagnostic method of fetal distress.
Among 76 pregnant women who underwent computerized electronic fetal monitoring and cord blood gas analysis, we divided them into three groups: normal fetus group (36); presumed distress group (26); and acidemic distress group (14). In order to perform linear analysis on the raw data of the fetal heart rate, after resampling, we performed Fourier transformation and investigated power distributions among very low frequency (VLF), low frequency (LF), high frequency (HF) bands, and autonomic balance (LF/HF).
The results of the spectral analysis showed that in normal fetus group, the difference in the distribution of power spectrums of VLF, LF and HF was significantly higher than in presumed distress group and acidemic distress group. In fetal distress, the LF and VLF value (< or = 0.0023, > or = 0.0437) were good predictors (sensitivity 97.5%, 75.0% and specificity 86.1%, 94.4%). The LF value (< or = 0.0013) was a good predictor in fetal acidemia (sensitivity 97.5% and specificity 86.1%).
A computerized spectral analysis of fetal heart rate variation is a good predictor of fetal distress, which is made automatically and objectively.
International Journal of Gynecology & Obstetrics 06/2001; 73(2):109-16. · 2.05 Impact Factor
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ABSTRACT: Adult intussusception is rare, and the majority of cases has an underlying cause that requires surgical resection. We report a case of a 39 yr-old man with ileo-ileo-colic intussusception caused by ileal lipoma that was successfully managed by a laparoscopic-assisted surgical maneuver. Using a three-cannula technique, ileo-colic intussusception was reduced laparoscopically. Then, through a 4-cm transverse incision in the right lower quadrant abdomen, ileo-ileal intussusception was reduced manually, and a resection of the tumor-bearing ileal segment and end-to-end anastomosis was performed extracorporeally. Although the role of laparoscopy in managing intussusception is not clearly defined, laparoscopy may be an alternative approach to the surgical treatment of adult intussusception in selected cases.
Journal of Korean Medical Science 03/2001; 16(1):119-22. · 0.99 Impact Factor
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ABSTRACT: The band structures of zincblende, hexagonal and wurtzite BN have been calculated using the full-potential linear augmented-plane-wave (FLAPW) method systematically. The present results for zincblende and hexagonal BN are compared with earlier calculations. For the wurtzite structure, the work is the first ab initio calculation. The possibility of inter-layer states of hexagonal BN playing a crucial role in intercalation problems-which are already being studied experimentally-is discussed on the basis of the variation of the energy levels with respect to the variation of the c axis: the Gamma 1+ inter-layer state becomes the minimum of the conduction band with about 15% expansion of the c axis. A total-energy calculation gives bulk moduli of 3.53 and 3.49 Mbar for zincblende and wurtzite structures, respectively.
Journal of Physics C Solid State Physics 11/2000; 20(9):1241.
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J. Nucl. Sci. (Seoul), 9: No. 1, 119-24(1969). 12/1968;
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J. Nucl. Sci. (Seoul), 9: No. 1, 125-32(1969). 12/1968;
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ABSTRACT: Using synchrotron radiation, photoelectron energy distribution curves in the valence band region have been measured on sintered polycrystalline YBa2Cu3O6.9 and YBa2Cu3O6.0 at liquid nitrogen temperature. Spectra measured on scraped clean surfaces are found to be reproducible. Overall spectra shapes coincide with the energy-band density-of-states curves calculated by the full potential linear augmented plane wave method, provided the locations of the bands are shifted by 1.0 eV. The Fermi edge is recognized in YBa2Cu3O6.9 but not in YBa2Cu3O6.0. The step at the Fermi edge is about one fiftieth as high as the highest peak at 2.3 eV.
Physica C: Superconductivity.
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N.H. Kwon,
S.H. Kim, K.T. Park,
W.K. Bae,
J.Y. Kim,
J.Y. Lim,
J.S. Ahn,
K.S. Lyoo,
J.M. Kim,
W.K. Jung,
K.M. Noh,
G.A. Bohach,
Y.H. Park
[show abstract]
[hide abstract]
ABSTRACT: The extended single-reaction multiplex PCR (esr-mPCR) developed in this study to detect staphylococcal enterotoxins (SEs), including SEA, SEB, SEC, SED, SEE, SEH, SEI, and SEJ, requires fewer sets of primers than other conventional multiplex PCRs and can be used to detect newly identified staphylococcal enterotoxins SEs more readily. Esr-mPCR analysis of 141 isolates of Staphylococcus aureus obtained from abattoir and livestock product samples revealed that 27 of the S. aureus isolates were toxigenic, and two were 2 multitoxigenic isolates. The most prevalent SE type was SEI followed by SEA and SEH. In addition, we investigated the clonal relatedness of toxigenic S. aureus isolates by arbitrarily primed PCR (AP-PCR). AP-PCR analysis of toxigenic S. aureus isolates revealed that the discriminatory power of AP-PCR was 9 (D=0.81), 8 (D=0.77), and 10 types (D=0.83) with primers AP1, ERIC2, and AP7, respectively. The combination of three each AP-PCR result could rearrange toxigenic S. aureus isolates into 10 types and five subtypes, with the D-value of 0.92. Interestingly, our data showed that toxigenic S. aureus isolates from different sources had different fingerprinting patterns although some of them carried the same types of SE genes. These data suggest that combinations of esr-mPCR and AP-PCR can provide a powerful approach for epidemiological investigation of toxigenic S. aureus isolates.
International Journal of Food Microbiology.
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[hide abstract]
ABSTRACT: The purpose of this study was to better understand the metallurgical transformations that occur during heat treatment of Nd-based magnets. DSC analysis and microstructural investigations of the grain boundaries with SEM and TEM were carried out. DSC permitted to optimize the heat treatment. Microstructural investigations always showed the presence of thin and smooth RE-rich film, associated with the highest coercivity.
Journal of Magnetism and Magnetic Materials.
