G I Abelev

N.N. Blokhin Cancer Research Center, Moskva, Moscow, Russia

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Publications (102)195.78 Total impact

  • Bulletin of Experimental Biology and Medicine 09/1981; 92(3):1237-1241. · 0.34 Impact Factor
  • A A Sokolenko, G I Abelev
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    ABSTRACT: Two-floor rocket-electrophoresis on gelatinated acetate cellulose membrane "Cellogel" has been developed. The method is based on electroimmunodiffusion detection of the antigen on the acetate-cellulose membranes, containing monospecific antiserum of the test-system. The procedure is followed by the detection of precipitation bands ("rockets") by staining, if the reaction is conducted in the visible zone or by the further treatment of the acetate-cellulose strips, containing invisible precipitates with antiglobulin antibodies, the complement or their combination. An increase in the method sensitivity up to 30-60 ng/ml in the visible zone of the reaction is achieved by simultaneous reduction in the antibody concentration and the growth of the absolute quantity of the antigen, subjected to electrophoresis, up to 50-100 microliters. The method has been applied to human alpha-fetoprotein.
    Biulleten' eksperimental'noĭ biologii i meditsiny 10/1979; 88(9):369-71.
  • G I Abelev, E R Karamova
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    ABSTRACT: A variant of counter-flow isotachophoresis of proteins on cellulose acetate membranes is proposed. The liquid counter-flow is created by electroendosmosis in the membrane. Proteins are concentrated at the Kolrausch boundary during isotachophoresis in the presence of ampholytes. The method permits one to make microanalysis of proteinic mixtures in diluted solutions, and it can be used in combination with immunodiffusion and immunoelectrophoretic methods of antigenic protein detection.
    Biulleten' eksperimental'noĭ biologii i meditsiny 10/1979; 88(9):334-6.
  • G. I. Abelev, É. R. Karamova
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    ABSTRACT: A method of counterflow isotachophoresis of proteins on cellulose acetate films is suggested. To produce a counterflow of fluid, an electroendosmotic flow formed in the film during passage of an electric current through it was used. During isotachophoresis of proteins in the presence of ampholytes, the proteins are concentrated on the boundary between the leading and closing ions and, at the same time, they are fractionated by electrophoretic mobility. Microanalysis of protein mixtures in dilute solutions can be undertaken by this method. It is readily combined with immunodiffusion and immunoelectrophoretic detection of protein antigens.
    Bulletin of Experimental Biology and Medicine - BULL EXP BIOL MED-ENGL TR. 01/1979; 88(3):1049-1053.
  • G I Abelev, A A Sokolenko
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    ABSTRACT: A highly sensitive electro-immunodiffusion test suggested by the authors for antigen detection on cellulose-acetate films consists of three stages: antigen concentration in a discontinuous buffer system on cellulose-acetate films; antigen detection on the same films by immunodiffusion using standard test system; to detect the precipitation bands the washed films are stained with protein dyes in case the reaction takes place in the zone of vision, or subject to further treatment by means of "plating" the precipitates with antiglobulin antibody or by radioautography. The method permits one to reveal the nanogram levels of alfa-fetoprotein and it may be applied for detection of antigens with different molecular weights and electrophoretic mobility.
    Biulleten' eksperimental'noĭ biologii i meditsiny 08/1977; 84(7):121-4.
  • T L Eraĭzer, D A El'gort, G I Abelev
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    ABSTRACT: Population aspects of specific secreted proteins (alpha-fetoprotein and serum albumin) were analyzed in the cultured human embryo hepatocytes (6 to 12 weeks' gestation). A method based on local hemolysis in gel of sheep erythrocytes conjugated with antibodies specific of proteins in question. The great majority of individual hepatocytes synthesized both proteins.
    Biulleten' eksperimental'noĭ biologii i meditsiny 07/1977; 83(6):711-3.
  • S D Perova, T L Eraizer, G I Abelev
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    ABSTRACT: Alpha-fetoprotein (AFP) produced by individual hepatocytes and hepatocyte microcolonies was detected with microelectrophoresis-precipitation in polyacrylamide gel. Hepatic cells of 6--13-week-old human embryos were cultivated in vitro for 2 to 5 days. 23 of 28 individual cells, and 89 of 91 microcolonies, built up of 2--35 cells, were demonstrated to produce AFP within the range of 70--800 pg per cell.
    Biulleten' eksperimental'noĭ biologii i meditsiny 05/1977; 83(4):481-4.
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    ABSTRACT: Population aspects of the production of specific secreted proteins (serum albumin and a-fetoprotein) were studied in cultures of hepatocytes from human embryos at 6–12 weeks of development. A method based on local hemolysis in gel using sheep's erythrocytes conjugated with antibodies against the proteins for testing, was used. The overwhelming majority of hepatocytes were shownto synthesize both proteins.
    Bulletin of Experimental Biology and Medicine - BULL EXP BIOL MED-ENGL TR. 01/1977; 83(6):843-846.
  • S. D. Perova, T. L. Éraizer, G. I. Abelev
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    ABSTRACT: By a combination of microelectrophoresis and precipitation in polyacrylamide gel, a-fetoprotein (a-FP) produced by single hepatocytes and by microcolonies of hepatocytes was determined. Liver cells from 6–13-week-old human fetuses were cultivatedin vitro for 2–5 days. a-FP was found to be produced in amounts of between 70 and 800 pg per cell by 23 of 28 single hepatocytes and by 89 of 91 microcolonies consisting of 2 to 35 cells
    Bulletin of Experimental Biology and Medicine - BULL EXP BIOL MED-ENGL TR. 01/1977; 83(4):569-573.
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    ABSTRACT: alpha-FOETOPROTEIN (AFP) is a serum protein present in large amounts during embryonic life and preserved in only nanogram quantities in adult mammals1. Increased levels of AFP are seen in adults in association with two forms of tumours-teratocarcinomas and hepatocarcinomas-and also during liver regeneration2. In early development AFP is synthesised in the yolk sac and in the liver2,3 where it has been demonstrated by immunofluorescence in the majority of the hepatocytes4. Detailed aspects of AFP synthesis by hepatocytes, however, remain to be established. It has been suggested that it is synthesised by a definite population of hepatocytes5, by one of the stages in their differentiation6,7, or by liver cell precursors8. Sufficient data to decide between these alternatives are not presently available. We describe here the immunohistochemical localisation of AFP in regenerating mouse livers, in which DNA-synthesising cells have been identified by thymidine incorporation. AFP was found in a minority of adult differentiated hepatocytes, many of which had not progressed through S phase. Thus DNA synthesis is not a prerequisite for the induction of AFP synthesis in the adult liver.
    Nature 10/1976; 263(5573):146-8. · 38.60 Impact Factor
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    ABSTRACT: A new antigan has been revealed by means of antisera against Rauscher virus in mice with Rauscher virus-induced leukemia. This antigen appears to be different from both Rauscher type-specific antigen and MULV-gs-1 (p-30), as shown by studies of electrophoretic mobility and immunochemical specificity. Except in leukemic mice it was also found in low levels in both serum and spleen extracts of healthy mice of a number of strains. Furthermore, this antigen was regularly demonstrated by immunofluorescence on the surface of erythroblasts, but not on the surface of erythrocytes, lymphocytes, polymorphonuclear cells and thymocytes, and was shown to be different from fetal hemoglobin. Therefore, it is referred to as antigen of erythroblasts (Ag-Eb), which seems to represent a surface marker for a certain differentiation stage of erythroid cells.
    International Journal of Cancer 07/1976; 17(6):798-805. · 6.20 Impact Factor
  • A A Sokolenko, G I Abelev
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    ABSTRACT: Alpha-fetoprotein (AFP) was detected in the sera of adult healthy persons by the method of electrophoresis-precipitation in polyacrylamide gel; it was distinctly determined in about 50% of cases, and its concentration was not over 3ng/ml. AFP was determined all the 20 cases suffering from cirrhosis of the liver examined by the mentioned method.
    Biulleten' eksperimental'noĭ biologii i meditsiny 04/1976; 81(3):354-5.
  • G I Abelev, S D Petrova, A A Sokolenko
    Annals of the New York Academy of Sciences 02/1976; 276:75-90. · 4.38 Impact Factor
  • Vestnik Akademii meditsinskikh nauk SSSR 02/1976;
  • A. A. Sokolenko, G. I. Abelev
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    ABSTRACT: Alpha-fetoprotein (AFP) was detected by combined electrophoresis and precipitation in polyacrylamide gel (EPAG) in the sera of healthy blood donors. AFP was found in about half of the sera in concentrations of not more than 3 ng/ml. AFP could not be reliably determined in the other cases, the results being at the limit of sensitivity of the method. EPAG thus gives results fully comparable in sensitivity with the radioimmunological test and it fully retains the resolving power of double diffusion in gel. EPAG can be used to verify the specificity of the results of the radioimmunological test.
    Bulletin of Experimental Biology and Medicine - BULL EXP BIOL MED-ENGL TR. 01/1976; 81(3):423-425.
  • G I Abelev, S D Perova
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    ABSTRACT: A method of alpha-fetoprotein (AFP) ultramicrodetection, permitting to reveal up to 10(--8) mg of AFP with its concentration of 1--2.10(--6) mg/ml was suggested. The antigen ic concentrated in a capillary tube during the electrophoresis in polyacrylamide gel. The compressed zone moves into the gel plate, containing antiserum. Free diffusion of the antigen is followed by the formation of precipitation rings, whose area is in proportion to the amount of the antigen. The precipitins are revealed by the treatment with I125-labeled antibodies to gamma-globulin of the immune serum, with subsequent radioautography.
    Biulleten' eksperimental'noĭ biologii i meditsiny 07/1975; 79(6):120-3.
  • Bulletin of Experimental Biology and Medicine 01/1975; 77(6):673-7. · 0.34 Impact Factor
  • G. I. Abelev, S. D. Perova
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    ABSTRACT: An ultramicromethod for determining a-fetoprotein (AFP), capable of detecting as little as 10-8 mg AFP in a concentration of (1–2) · 10-6 mg/ml, is suggested. The antigen is concentrated in a capillary tube by electrophoresis in polyacrylamide gel. The concentrated zone migrates into the slab of gel containing antiserum. After free diffusion of the antigen, precipitation rings are formed and their area is proportional to the quantity of antigen. The precipitates are revealed by treatment with I125-antibodies against immune serum ?-globulin followed by autoradiography.
    Bulletin of Experimental Biology and Medicine - BULL EXP BIOL MED-ENGL TR. 01/1975; 79(6):718-721.
  • Vestnik Akademii meditsinskikh nauk SSSR 02/1974;
  • G I Abelev
    Transplantation reviews 02/1974; 20:3-37. · 2.66 Impact Factor

Publication Stats

2k Citations
195.78 Total Impact Points

Institutions

  • 1998–2008
    • N.N. Blokhin Cancer Research Center
      Moskva, Moscow, Russia
    • McGill University
      • Department of Medicine
      Montréal, Quebec, Canada
  • 1994–2008
    • Russian Academy of Sciences
      • The N. N. Blokhin Cancer Research Center
      Moskva, Moscow, Russia
  • 1967–2002
    • Russian Academy of Medical Sciences
      Moskva, Moscow, Russia
  • 1970–1976
    • Research Institute of Epidemiology and Microbiology n.a. N. F. Gamalei, Russian Academy of Medical Sciences
      Moskva, Moscow, Russia
    • International Agency for Research on Cancer
      Lyons, Rhône-Alpes, France