Doo-Sang Park

Korea Research Institute of Bioscience and Biotechnology KRIBB, Anzan, Gyeonggi Province, South Korea

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Publications (39)71.95 Total impact

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    ABSTRACT: A Gram-negative, aerobic, non-motile and rod-shaped bacterial strain, D-17(T), was isolated from mud flats in the Yellow Sea in Korea. Phylogenetic trees based on the 16S rRNA gene sequence showed that strain D-17(T) belongs to the genus Pseudoruegeria and it shared 97.5 % similarity with the type strain of Pseudoruegeria haliotis WM67(T). The sequence similarities with Pseudoruegeria litimaris HD-43(T) and Pseudoruegeria aquimaris SW-255(T) were 96.9 and 96.1 %, respectively. Strain D-17(T) was found to grow with 0.5-6 % (w/v) NaCl, at 20-30 °C, and at pH 6.5-8.0. Strain D-17(T) was determined to contain Q-10 as the predominant ubiquinone and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c, as defined by the MIDI system) as the major fatty acids. The major polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminolipid, an unidentified glycolipid, an unidentified lipid and four unidentified phospholipids. The DNA G+C content was determined to be 63.6 mol%. The DNA-DNA relatedness with P. haliotis WM67(T) was 32.5 %. The differential phenotypic properties revealed that strain D-17(T) can be separated from other Pseudoruegeria species. Based on the data presented in this study, strain D-17(T) represents a novel species, for which the name Pseudoruegeria limi sp. nov. is proposed. The type strain is D-17(T) (=KCTC 32460(T) =JCM 19487(T)).
    Antonie van Leeuwenhoek 03/2014; · 2.07 Impact Factor
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    ABSTRACT: A Gram-negative, non-motile and rod-shaped bacterial strain, designated RA2-3(T), was isolated from a sea squirt (Halocynthia roretzi) collected from the South Sea, Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain RA2-3(T) was observed to grow optimally at 25 °C, at pH 7.0-7.5 and in the presence of 2 % (w/v) NaCl. Strain RA2-3(T) exhibited the highest 16S rRNA gene sequence similarity values to the type strains of Litoreibacter meonggei (95.7 %), Planktotalea frisia (95.6 %), Thalassobius gelatinovorus (95.5 %) and Pelagicola litoralis (95.4 %). A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain RA2-3(T) clustered with the type strains of Planktotalea frisia, Pelagicola litoralis, Pacificibacter maritimus and Roseovarius marinus. Strain RA2-3(T) was found to contain Q-10 as the predominant ubiquinone and C18:1 ω7c as the major fatty acid. The major polar lipids detected in strain RA2-3(T) were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain RA2-3(T) was 52.9 mol%. On the basis of the phylogenetic, chemotaxonomic and phenotypic properties, strain RA2-3(T) is considered to represent a new genus and species within the family Rhodobacteraceae, for which the name Halocynthiibacter namhaensis gen. nov., sp. nov. is proposed. The type strain of H. namhaensis is RA2-3(T) (=KCTC 32362(T)=NBRC 109999(T)).
    Antonie van Leeuwenhoek 02/2014; · 2.07 Impact Factor
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    ABSTRACT: A Gram-stain-negative, non-motile and rod-shaped bacterial strain, AH-M5T, which was isolated from a tidal flat sediment at Aphae island in South Korea, was characterized taxonomically. Strain AH-M5T grew optimally at 25 °C, at pH 7.0-8.0 and in presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain AH-M5T clustered coherently with the type strains of Mangrovimonas yunxiaonensis and Meridianimaribacter flavus, showing 93.4-94.3 % sequence similarity. It exhibited 16S rRNA gene sequence similarity values of less than 93.4 % to the type strains of the other recognized species. Strain AH-M5T contained MK-6 as the predominant menaquinone and iso-C15:1 G, iso-C15:0, iso-C17:0 3-OH and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) as the major fatty acids. The major polar lipid profile of strain AH-M5T containing phosphatidylethanolamine and one unidentified lipid as major components was differentiated from those of the type strains of M. yunxiaonensis and M. flavus. The DNA G+C content of strain AH-M5T was 34.8 mol%. Differential phenotypic properties, together with the phylogenetic and chemotaxonomic data, demonstrated that strain AH-M5T is distinguished from M. yunxiaonensis and M. flavus. On the basis of the data presented, strain AH-M5T is considered to represent a novel genus and species within the family Flavobacteriaceae, for which the name Seonamhaeicola aphaedonensis gen. nov., sp. nov. is proposed. The type strain is AH-M5T (= KCTC 32578T = CECT 8487T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2014; · 2.11 Impact Factor
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    ABSTRACT: A novel strain, designated W-15T, was isolated from the gut of the long-horned beetle, Massicus raddei, collected in South Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belonged to the suborder Micrococcineae. The strain W-15T was most closely related to Luteimicrobium album RI148-Li105T (97.9% similarity). Strain W-15T was Gram- positive, rod and coccus shaped, non-motile. Growth was observed at 15-37 °C, at pH 4.5-8.5 and in the presence of 0-5.0% NaCl. The cell-wall peptidoglycan of the strain was A4α (L-Lys-D-Ser-D-Asp). The major menaquinone present in this strain was MK-8 (H2) and the major cellular fatty acids were anteiso-C15:0, iso-C16:0, iso-C15:0 and anteiso-C17:0. The major polar lipids were diphosphatidylglycerol, phosphatidylinositol, an unknown lipid an unknown phospholipid and an unknown phosphoglycolipid. The G+C content of genomic DNA of the strain was 73.8%. On the basis of evidence from our polyphasic taxonomic study, strain W-15T is classified within a novel genus and species in the suborder Micrococcineae, for which the name Luteimicrobium xylanilyticum sp. nov. is proposed. The type strain of this genus is strain W-15T (= KCTC 19882 T = JCM 18090 T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2014; · 2.11 Impact Factor
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    ABSTRACT: A Gram-negative, aerobic, non-spore-forming, non-flagellated and rod-shaped or ovoid bacterial strain, designated SSK6-1T, was isolated from the zone where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain SSK6-1T grew optimally at 30 °C, at pH 7.0-7.5 and in the presence of 2.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences revealed that strain SSK6-1T clustered with the type strains of Phaeobacter daeponensis and Phaeobacter caeruleus, exhibiting sequence similarities of 98.6 and 98.3 %, respectively. The novel strain exhibited sequence similarities of 94.6-97.8 % to the type strains of the other Phaeobacter species and Leisingera species. Strain SSK6-1T contained Q-10 as the predominant ubiquinone and C18:1 ω7c and 11-methyl C18:1 ω7c as the major fatty acids. The major polar lipids of strain SSK6-1T were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain SSK6-1T was 64.6 mol% and its DNA-DNA relatedness values with P. daeponensis TF-218T and P. caeruleus LMG 24369T were 21 and 25 %, respectively. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain SSK6-1T is separate from other Phaeobacter species. On the basis of the data presented, strain SSK6-1T is considered to represent a novel species of the genus Phaeobacter, for which the name Phaeobacter aquaemixtae sp. nov. is proposed. The type strain is SSK6-1T (= KCTC 32538T = CECT 8399T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2014; · 2.11 Impact Factor
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    ABSTRACT: Habitat changes of butterflies are caused by various factors, including food plants, natural enemies, and climate change. Recently, new habitats of the large copper have been reported on the Korean Peninsula, mostly along a riverside. This implies that this butterfly may move along a drainage system. In this study, new habitats of the large copper along the Geum River were investigated and the population dynamics of the butterfly were monitored in selected areas through the mark–release–recapture method. The results showed that the large copper had dispersed inland along the Geum River, which runs along the central western seashore of South Korea. In addition, the habitat distribution of the butterfly showed good coincidence with the distribution of a sorrel, Rumex japonicas, the main food plant of the large copper. A study on the moving distance of the butterfly showed that females moved further than males, while males showed more frequent movement within a short distance. The movement of the large copper along the Gum River, which lies over 100 km south of the Han River (a habitat previously reported in 1973), suggests that the movement of the butterfly might be more affected by human activities, such as the development of bike paths along the Gum River and maintenance of the riverbed, than climate change.
    Journal of Asia-Pacific Entomology 01/2014; · 0.80 Impact Factor
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    ABSTRACT: A novel facultatively anaerobic strain DH1(T) was isolated from deep sub-seafloor sediment at a depth of 900 m below the seafloor off Seo-do (the west part of Dokdo Island) in the East Sea of the Republic of Korea. The new strain was characterized using polyphasic approaches. The isolate was Gram-stain-negative, motile by gliding, non-spore-forming rods, oxidase-negative, and catalase-positive; and formed colonies of orange-red color. The NaCl range for growth was 0.5-7.0% (w/v) and no growth was observed in the absence of NaCl. The isolate grew optimally at 30°C, with 2% (w/v) NaCl and at pH 7. The cell-wall hydrolysates contained ribose as a major sugar. The DNA G+C content was 40.8 mol%. The closest related strains are Sunxiuqinia faeciviva JAM-BA0302(T) and Sunxiuqinia elliptica DQHS-4(T) (97.9 and 96.3% sequence similarity, respectively). The level of DNA-DNA relatedness between strain DH1(T) and S. faeciviva JAM-BA0302(T) was around 41% (but only 6% between DH1T and S. elliptica DQHS-4(T)). The major cellular fatty acids of the isolate were contained iso-C15:0 (25.9%), anteiso-C15:0 (16.7%), and summed feature 9 (comprising C16:0 3-OH and/or unknown fatty acid of dimethylacetal ECL 17.157; 13.2%). The predominant menaquinone was MK-7. On the basis of polyphasic evidence from this study, the isolate was considered to represent a novel species of the genus Sunxiuqinia, for which the name Sunxiuqinia dokdonensis sp. nov. is proposed; the type strain is DH1(T) (=KCTC 32503(T) =CGMCC 1.12676(T) =JCM 19380(T)).
    The Journal of Microbiology 12/2013; 51(6):741-6. · 1.28 Impact Factor
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    ABSTRACT: A Gram-staining-negative, non-motile, catalase- and oxidasepositive bacterium, designated strain I-15(T), was isolated from a crab of the Yellow Sea, Korea. On the basis of a 16S rRNA gene sequence analysis, strain I-15(T) was shown to belong to Bacteroidetes, related to the genus Aestuariibaculum. Sequence similarity between strain I-15(T) and the only type strain of the genus Aestuariibaculum, Aestuariibaculum suncheonense SC17(T), was 96.7%. Strain I-15(T) grew at 0.5-6.0% (w/v) NaCl, at 10-42°C and at pH 4.5-8.0. It could hydrolyze starch and Tweens 80. Menaquinone-6 was the only respiratory quinone, and summed features 3 (C16:1 ω7c/C16:1 ω6c) (16.4%), iso-C15:0 (15.6%), and iso-C15:1 G (12.6%) were the major cellular fatty acids. The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. The DNA G+C content was 39.0 mol%. Polyphasic data allowed genotypic and phenotypic distinction of strain I-15(T) from the only validly published Aestuariibaculum species. Therefore, the organism is considered a novel species of the genus Aestuariibaculum, for which the name Aestuariibaculum scopimerae sp. nov. is proposed. The type strain is I-15(T) (=KCTC 32459(T) =JCM 19486(T)).
    The Journal of Microbiology 12/2013; 51(6):736-40. · 1.28 Impact Factor
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    ABSTRACT: A Gram-staining-negative, catalase- and oxidase-positive bacterium, non-motile, designated strain KOPRI 21160T, was isolated from Antarctic soil. Based on 16S rRNA gene sequence analysis, strain KOPRI 21160T was found to belong to the genus Polaribacter. Sequence similarity between strain KOPRI 21160T and the type strains of Polaribacter species was 94.2-98.3%. The nearest phylogenetic neighbors of strain KOPRI 21160T were Polaribacter butkevichii KCTC 12100T (98.3%) and Polaribacter irgensii KCTC 23136T (97.5%). The DNA-DNA relatedness value between strain KOPRI 21160T and P. butkevichii KCTC 12100T was 50.6%, and 45.2% between strain KOPRI 21160T and P. irgensii KCTC 23136T. Strain KOPRI 21160T grew at 4-37°C and at pH 7.0-8.5. It could hydrolyze DNA, starch and Tweens 20, 40, 60 and 80. Menaquinone-6 (MK-6) was the only respiratory quinone, and iso-C15:0 (18.1%), iso-C15:0 3OH (17.9%) and C15:1 ω6c (10.4%) were the major cellular fatty acids. The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and one unidentified lipid. The DNA G+C content was 30.0 mol%. Based on polyphasic data, the organism is considered as a novel species of the genus Polaribacter, for which we propose the name Polaribacter sejongensis sp. nov. The type strain is KOPRI 21160T (= KCTC 23670T = JCM 18092T). Emended descriptions of the genus Polaribacter, Polaribacter butkevichii Nedashkovskaya et al. 2005 and Polaribacter irgensii Gosink et al. 1998 are also proposed.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 05/2013; · 2.11 Impact Factor
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    ABSTRACT: Zebrafish is considered as a versatile experimental animal for various research models from development to diseases. In this study, we report the development of transgenic zebrafish line named as Tg(EF1α:Kaede) that expresses translation elongation factor 1 subunit alpha (EF1α) promoter linked to a fluorescent protein (FP), Kaede for monitoring proliferating cells in during regeneration. It was revealed that about 1.4 kb 5'-flanking region of the EF1α was sufficient for its promoter activity. Expression of Kaede with a property of photo-conversion from green to red was detected in different embryonic stages as well as various organs such as brain, heart, pancreas, intestine, ovary, and fins of adult fish. Cell proliferation pattern during fin regeneration was monitored after amputation of Tg(EF1α:Kaede) caudal fin and results shown that this system is simple and efficient method for detecting proliferating cells during tissue regeneration. Developed Tg(EF1α:Kaede) line has potential to investigate the cell proliferation, regeneration, wound healing capacities after tissue damage and evaluate the therapeutic power of wound healing drugs.
    Fish &amp Shellfish Immunology 03/2013; · 2.96 Impact Factor
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    ABSTRACT: We report the 3.74-Mb genome sequence of Galloisinimonas intestini B14(T), isolated from the gut of one of the world's rarest insect species, Galloisiana sp., collected at a Mosan cave, Moonkyung, Gyungsangbook-do, South Korea. Strain B14(T) is a novel genus candidate of the family Enterobacteriaceae.
    Journal of bacteriology 12/2012; 194(23):6648. · 3.94 Impact Factor
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    ABSTRACT: Two novel Gram-staining-negative, motile by gliding, rod-shaped bacteria, strains N7d-4T and B4a-b5, were isolated during a study of culturable bacteria in soil cultivating potatoes. These isolates grew at 15-37 °C and pH 6.5-7.0. The major components of their cellular fatty acids were summed feature 3 (iso-C15:0 2-OH and/or C16:1 ω7c; 9.8-16.9 %), anteiso-C15:0 (13.6-16.5 %), iso-C15:0 (11.5-16.0 %), iso-C17:0 3-OH (9.6-15.1 %) and iso-C17:1 ω9c (6.9-7.7 %). The DNA G + C content of the genomic DNA was 46.9-48.5 mol% (HPLC). A phylogenetic analysis based on 16S rRNA gene sequences showed that strains N7d-4T and B4a-b5 were affiliated with Pedobacter species in the family Sphingobacteriaceae. Strains N7d-4T and B4a-b5 shared 99.9 % sequence similarity and the most closely related Pedobacter species were Pedobacter composti TR6-06T (96.5 and 96.7 %, respectively), Pedobacter oryzae N7T (95.4 and 95.6 %) and Pedobacter caeni LMG 22862T (94.0 and 94.4 %). The phenotypic and phylogenetic characteristics of the isolates allowed these two isolates to be clearly distinguished from other Pedobacter species. Based on these data, the isolates are considered to represent a novel species of the genus Pedobacter, for which the name Pedobacter luteus sp. nov. is proposed. The type species is N7d-4T (= KCTC 22699 T = DSM 22385T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 07/2012; · 2.11 Impact Factor
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    ABSTRACT: Two novel, Gram-positive, motile, coccal bacteria, strains L1b-b9(T) and B5a-b5, were isolated from a potato cultivation field in Ochang, Korea. These isolates grew at 10-45°C, pH 5.0-10.0, and in the presence of 8% (w/v) NaCl. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major menaquinone was MK-8(H(4)) and the main cellular fatty acids were iso-C(14:0), iso-C(15:0), and anteiso-C(15:0). Polar lipids in strain L1b-b9(T) consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and an unknown glyco-amino lipid. The G+C content of genomic DNA was 73.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strains L1b-b9(T) and B5a-b5 shared 99.36% similarity and formed a robust clade with the type species of the genus Phycicoccus. Strain L1b-b9(T) is related most closely to Phycicoccus cremeus V2M29(T) (97.52% 16S rRNA gene sequence similarity). On the basis of phylogenetic characteristics, the name Phycicoccus ochangensis sp. nov. is proposed for strain LIb-b9(T) (=KCTC 19694(T) =JCM 17595(T)).
    The Journal of Microbiology 04/2012; 50(2):349-53. · 1.28 Impact Factor
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    ABSTRACT: Strains RU-16(T), RU-28, RU-04(T) and PU-02(T) were isolated from the gut of the African mole cricket, Gryllotalpa africana. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the strains belonged to the family Microbacteriaceae. All four strains were most closely related to Curtobacterium ginsengisoli DCY26(T) (below 97 % 16S rRNA gene sequence similarity). These isolates were Gram-stain-positive, motile (by gliding), rod-shaped and exhibited ivory-coloured colonies. Their chemotaxonomic properties included MK-11 as the major respiratory quinone, ornithine as the cell-wall diamino acid, acetyl as the acyl type of the peptidoglycan, cyclohexyl-C(17 : 0) as the major fatty acid and phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids. On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, we propose a new genus in the family Microbacteriaceae, Gryllotalpicola gen. nov., with three novel species, Gryllotalpicola daejeonensis sp. nov. (type strain RU-04(T)  = KCTC 13809(T)  = JCM 17590(T)), Gryllotalpicola koreensis sp. nov. (type strain RU-16(T)  = KCTC 13810(T)  = JCM 17591(T)) and Gryllotalpicola kribbensis sp. nov. (type strain PU-02(T)  = KCTC 13808(T)  = JCM 17593(T)). Gryllotalpicola koreensis is the type species of the genus. Additionally, we propose that Curtobacterium ginsengisoli should be reclassified in the genus as Gryllotalpicola ginsengisoli comb. nov. (type strain DCY26(T)  = KCTC 13163(T)  = JCM 14773(T)).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 11/2011; 62(Pt 10):2363-70. · 2.11 Impact Factor
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    ABSTRACT: During a study intended to screen for agar-degrading bacteria, strain M2-5T was isolated from black sand off the shore of Jeju Island, Republic of Korea. Strain M2-5T exhibited agarase activity; the β-agarase gene of the isolate had 62 % amino acid sequence identity to the β-agarase gene of Microbulbifer thermotolerans JAMB A94T. The isolate was closely related to members of the genus Simiduia but was clearly discernible from reported Simiduia species, based on a polyphasic analysis. Cells of strain M2-5T were Gram-negative, catalase- and oxidase-positive, motile rods. The DNA G+C content was 53.3 mol%. The predominant isoprenoid quinone was Q-8. The major cellular fatty acids were C17:1ω8c (25.9 %), summed feature 3 (iso-C(15 : 0) 2-OH and/or C16:1ω7c; 17.2 %) and C17:0 (15.0 %). Phylogenetic analysis using 16S rRNA gene sequences showed that strain M2-5T had 96.6 % gene sequence similarity to Simiduia agarivorans SA1T, the most closely related type strain of the genus Simiduia. These results suggest that strain M2-5T represents a novel species in the genus Simiduia, for which the name Simiduia areninigrae sp. nov. is proposed; the type strain is M2-5T (=KCTC 23293T=NCAIM B 02424T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 06/2011; 62(Pt 4):906-11. · 2.11 Impact Factor
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    ABSTRACT: A novel Gram-positive, non-motile, rod-shaped bacterium, designated strain RB-62(T), was isolated during a study of culturable bacteria from the gut of Moechotypa diphysis (Pascoe) and its taxonomic position was investigated. Strain RB-62(T) grew at 15-30 °C and pH 5.0-8.5. The isoprenoid quinones were menaquinones MK-11 (77.1%), MK-10 (11.7%) and MK-12 (11.2%). The major cellular fatty acids were anteiso-C(15:0) (34.6%), anteiso-C(17:0) (29.8%), iso-C(16:0) (17.0%) and cyclohexyl-C(17:0) (11.4%). The diagnostic diamino acid of the cell-wall peptidoglycan was 2,4-diaminobutyric acid. The G+C content of the genomic DNA of strain RB-62(T) was 70.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RB-62(T) was affiliated with the genus Herbiconiux cluster within the family Microbacteriaceae, and was related most closely to Herbiconiux ginsengi wged11(T) (98.08% similarity). The level of DNA-DNA relatedness between strain RB-62(T) and H. ginsengi wged11(T) was 43.2% (reciprocal 66.7%). Phenotypic and phylogenetic characteristics clearly distinguished strain RB-62(T) from recognized species of the genus Herbiconiux. Based on data from the present polyphasic study, strain RB-62(T) is considered to represent a novel species of the genus Herbiconiux, for which the name Herbiconiux moechotypicola sp. nov. is proposed. The type strain is RB-62(T) (=KCTC 19653(T)=JCM 16117(T)).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2011; 62(Pt 1):90-5. · 2.11 Impact Factor
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    ABSTRACT: DNA barcoding, the analysis of sequence variation in the 5' region of the mitochondrial cytochrome c oxidase I (COI) gene, has been shown to provide an efficient method for the identification of species in a wide range of animal taxa. In order to assess the effectiveness of barcodes in the discrimination of Heteroptera, we examined 344 species belonging to 178 genera, drawn from specimens in the Canadian National Collection of Insects. Analysis of the COI gene revealed less than 2% intra-specific divergence in 90% of the taxa examined, while minimum interspecific distances exceeded 3% in 77% of congeneric species pairs. Instances where barcodes fail to distinguish species represented clusters of morphologically similar species, except one case of barcode identity between species in different genera. Several instances of deep intraspecific divergence were detected suggesting possible cryptic species. Although this analysis encompasses 0.8% of the described global fauna, our results indicate that DNA barcodes will aid the identification of Heteroptera. This advance will be useful in pest management, regulatory and environmental applications and will also reveal species that require further taxonomic research.
    PLoS ONE 01/2011; 6(4):e18749. · 3.73 Impact Factor
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    ABSTRACT: A novel ascomycetous yeast, designated strain N7a-Y2(T), was isolated from soil collected in a potato field in Ochang, Korea, and its taxonomic position was studied. A neighbour-joining tree based on the D1/D2 domain of large-subunit rRNA gene sequences revealed that the isolate was a member of the Wickerhamomyces clade and that it was closely related to Wickerhamomyces bisporus, Candida quercuum, Candida ulmi and Wickerhamomyces alni. Strain N7a-Y2(T) formed Saturn-shaped ascospores in unconjugated and persistent asci. D1/D2 domain 26S rRNA gene sequence divergences of 11.0-21.1 % between strain N7a-Y2(T) and other members of the Wickerhamomyces clade indicate that the strain represents a novel species of the genus Wickerhamomyces, for which the name Wickerhamomyces ochangensis sp. nov. is proposed. The type strain is N7a-Y2(T) ( = KCTC 17870(T)  = CBS 11843(T)).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 11/2010; 61(Pt 10):2543-6. · 2.11 Impact Factor
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    ABSTRACT: Mealybugs are under a strict regulation at foreign trades of agricultural products because they are one of the most economically damaging groups of insects on food crops and ornamental plants. However, the absence of morphological characteristics enabling the discrimination of early life stages often cause a significant delay or rejection of a shipment when infested fruit is discovered, causing significant economic loss. A polymerase chain reaction-based method for species identification was developed for six mealybug species known to infest Korean pears including two regulated insects, Planococcus kraunhiae (Kuwana) and Crisicoccus matsumotoi (Siraiwa). Six sets of species-specific primers were designed based on the sequence comparison of the internal transcribed spacer 1 and 2 regions. Efficiency tests against 29 mealybug samples showed that this method could effectively discriminate different mealybug species regardless of their developmental stages. Blind tests against 11 field collected mealybug nymph samples indicated that a single polymerase chain reaction is enough to discriminate unidentified mealybugs collected on Korean pears. This new method will facilitate trade and export requirements, as well as identify the species at any stage of mealybug intercepted.
    Journal of Economic Entomology 02/2010; 103(1):25-33. · 1.60 Impact Factor
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    ABSTRACT: A novel GH10 endo-β-1,4-xylanase (XylG) gene from Streptomyces thermocarboxydus HY-15, which was isolated from the gut of Eisenia fetida, was cloned, over-expressed, and characterized. The XylG gene (1182 bp) encoded a polypeptide of 393 amino acids with a deduced molecular mass of 43,962 Da and a calculated pI of 6.74. The primary structure of XylG was 69% similar to that of Thermobifida fusca YX endo-β-1,4-xylanase. It was most active at pH 6.0 and 55 °C. The susceptibilities of xylans to XylG were as follows: oat spelt xylan > birchwood xylan > beechwood xylan. The XylG also showed high activity (474 IU/mg) toward p-nitrophenylcellobioside. Moreover, at pH 6.0 and 50 °C, the Vmax and Km values of the XylG were 127 IU/mg and 2.51 mg/ml, respectively, for oat spelt xylan and 782 IU/mg and 5.26 mM, respectively, for p-nitrophenylcellobioside. A homology model indicated that XylG folded to form a (β/α)8-barrel with two catalytic residues of an acid/base (Glu181) and a nucleophile (Glu289). The formation of a disulfide bond between Cys321 and Cys327 were predicted by homology modeling.
    Journal of Molecular Catalysis B: Enzymatic. 01/2010;

Publication Stats

133 Citations
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71.95 Total Impact Points

Institutions

  • 2001–2014
    • Korea Research Institute of Bioscience and Biotechnology KRIBB
      • • Korea Research Institute of Bioscience and Biotechnology (KRIBB)
      • • Biological Resource Center
      • • Insect Resources Laboratory
      Anzan, Gyeonggi Province, South Korea
  • 2012
    • Iranian Biological Resource Center
      Teheran, Tehrān, Iran