Doo-Sang Park

Korea Research Institute of Bioscience and Biotechnology KRIBB, Anzan, Gyeonggi Province, South Korea

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Publications (48)76.17 Total impact

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    ABSTRACT: A novel bacterium, designated strain 13-2-B6(T), was isolated from seawater adjacent to Songak Mountain on Jeju Island, South Korea. The novel strain was observed to be Gram-negative, aerobic, rod-shaped and motile with a single polar flagellum. On the basis of 16S rRNA gene sequence similarity, strain 13-2-B6(T) was determined to be phylogenetically closely related to the type strain of Antarctobacter heliothermus, currently the sole species of the genus Antarctobacter (family Rhodobacteraceae). Sequence similarity between the 16S rRNA genes of strain 13-2-B6(T) and A. heliothermus EL-219(T) is 96.9 %. Strain 13-2-B6(T) was found to grow optimally at 25-30 °C, pH 7.0-8.0 and 3 % (w/v) NaCl. The predominant isoprenoid quinone in strain 13-2-B6(T) was identified as ubiquinone Q-10 and the major fatty acids were identified as C18:1 ω7c and/or C18:1 ω6c. Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unknown aminolipids, two unknown phospholipids, an unknown glycolipid and an unknown lipid were found to be components of the polar lipid profile. The G + C content of strain 13-2-B6(T) was determined to be 62 mol %. On the basis of its phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain 13-2-B6(T) is considered to represent a novel species of the genus Antarctobacter, for which the name Antarctobacter jejuensis sp. nov. is proposed. The type strain is 13-2-B6(T) (=KCTC 42009(T) =JCM 19898(T)).
    Antonie van Leeuwenhoek 09/2014; · 2.07 Impact Factor
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    ABSTRACT: A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated GJSW-31(T), was isolated from a seawater in the South Sea, South Korea. The novel strain grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain GJSW-31(T) clustered with the type strains of Litoreibacter species. Strain GJSW-31(T) exhibited 16S rRNA gene sequence similarity values of 95.2-98.5 % to the type strains of Litoreibacter species and sequence similarities of less than 96.18 % to the type strains of the other recognized species. Strain GJSW-31(T) contained Q-10 as the predominant ubiquinone and C18:1 ω7c as the major fatty acid. The major polar lipids of strain GJSW-31(T) were phosphatidylcholine, phosphatidylglycerol, one unidentified lipid and one unidentified aminolipid. The DNA G+C content of strain GJSW-31(T) was 62.5 mol% and its DNA-DNA relatedness values with the type strains of L. albidus, L. janthinus, L. meonggei and L. ascidiaceicola were 13-23 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain GJSW-31(T) is separated from other Litoreibacter species. On the basis of the data presented, strain GJSW-31(T) is considered to represent a novel species of the genus Litoreibacter, for which the name Litoreibacter ponti sp. nov. is proposed. The type strain is GJSW-31(T) (= KCTC 42114(T) = NBRC 110379(T)).
    International journal of systematic and evolutionary microbiology. 08/2014;
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    ABSTRACT: A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, BS-B2T, which was isolated from a tidal flat sediment at Boseong in South Korea, was characterized taxonomically. Strain BS-B2T grew optimally at 30 °C, at pH 7.0-8.0 and in presence of 2.0 % (w/v) NaCl. The novel strain exhibited the highest 16S rRNA gene sequence similarity (97.4 %) to the type strain of Marivita geojedonensis. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain BS-B2T is closely related to the type strain of Primorskyibacter sedentarius, showing 96.5 % sequence similarity. Strain BS-B2T contained Q-10 as the predominant ubiquinone and C18:1 ω7c as the predominant fatty acid. The major polar lipid profile of strain BS-B2T containing phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid as major components was differentiated from those of the type strains of P. sedentarius and M. geojedonensis. The DNA G+C content of strain BS-B2T was 62.2 mol%. Differential phenotypic properties, together with the phylogenetic and chemotaxonomic data, demonstrated that strain BS-B2T is distinguished from some phylogenetically related genera as well as P. sedentarius and M. geojedonensis. On the basis of the data presented, strain BS-B2T is considered to represent a novel genus and species, for which the name Aestuariivita boseongensis gen. nov., sp. nov. is proposed. The type strain is BS-B2T (= KCTC 42052T = CECT 8532T).
    International journal of systematic and evolutionary microbiology. 06/2014;
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    ABSTRACT: A Gram-negative, aerobic, non-motile and rod-shaped bacterial strain, D-17(T), was isolated from mud flats in the Yellow Sea in Korea. Phylogenetic trees based on the 16S rRNA gene sequence showed that strain D-17(T) belongs to the genus Pseudoruegeria and it shared 97.5 % similarity with the type strain of Pseudoruegeria haliotis WM67(T). The sequence similarities with Pseudoruegeria litimaris HD-43(T) and Pseudoruegeria aquimaris SW-255(T) were 96.9 and 96.1 %, respectively. Strain D-17(T) was found to grow with 0.5-6 % (w/v) NaCl, at 20-30 °C, and at pH 6.5-8.0. Strain D-17(T) was determined to contain Q-10 as the predominant ubiquinone and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c, as defined by the MIDI system) as the major fatty acids. The major polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminolipid, an unidentified glycolipid, an unidentified lipid and four unidentified phospholipids. The DNA G+C content was determined to be 63.6 mol%. The DNA-DNA relatedness with P. haliotis WM67(T) was 32.5 %. The differential phenotypic properties revealed that strain D-17(T) can be separated from other Pseudoruegeria species. Based on the data presented in this study, strain D-17(T) represents a novel species, for which the name Pseudoruegeria limi sp. nov. is proposed. The type strain is D-17(T) (=KCTC 32460(T) =JCM 19487(T)).
    Antonie van Leeuwenhoek 03/2014; · 2.07 Impact Factor
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    ABSTRACT: A Gram-negative, non-motile and rod-shaped bacterial strain, designated RA2-3(T), was isolated from a sea squirt (Halocynthia roretzi) collected from the South Sea, Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain RA2-3(T) was observed to grow optimally at 25 °C, at pH 7.0-7.5 and in the presence of 2 % (w/v) NaCl. Strain RA2-3(T) exhibited the highest 16S rRNA gene sequence similarity values to the type strains of Litoreibacter meonggei (95.7 %), Planktotalea frisia (95.6 %), Thalassobius gelatinovorus (95.5 %) and Pelagicola litoralis (95.4 %). A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain RA2-3(T) clustered with the type strains of Planktotalea frisia, Pelagicola litoralis, Pacificibacter maritimus and Roseovarius marinus. Strain RA2-3(T) was found to contain Q-10 as the predominant ubiquinone and C18:1 ω7c as the major fatty acid. The major polar lipids detected in strain RA2-3(T) were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain RA2-3(T) was 52.9 mol%. On the basis of the phylogenetic, chemotaxonomic and phenotypic properties, strain RA2-3(T) is considered to represent a new genus and species within the family Rhodobacteraceae, for which the name Halocynthiibacter namhaensis gen. nov., sp. nov. is proposed. The type strain of H. namhaensis is RA2-3(T) (=KCTC 32362(T)=NBRC 109999(T)).
    Antonie van Leeuwenhoek 02/2014; · 2.07 Impact Factor
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    ABSTRACT: A Gram-stain-negative, non-motile and rod-shaped bacterial strain, AH-M5T, which was isolated from a tidal flat sediment at Aphae island in South Korea, was characterized taxonomically. Strain AH-M5T grew optimally at 25 °C, at pH 7.0-8.0 and in presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain AH-M5T clustered coherently with the type strains of Mangrovimonas yunxiaonensis and Meridianimaribacter flavus, showing 93.4-94.3 % sequence similarity. It exhibited 16S rRNA gene sequence similarity values of less than 93.4 % to the type strains of the other recognized species. Strain AH-M5T contained MK-6 as the predominant menaquinone and iso-C15:1 G, iso-C15:0, iso-C17:0 3-OH and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) as the major fatty acids. The major polar lipid profile of strain AH-M5T containing phosphatidylethanolamine and one unidentified lipid as major components was differentiated from those of the type strains of M. yunxiaonensis and M. flavus. The DNA G+C content of strain AH-M5T was 34.8 mol%. Differential phenotypic properties, together with the phylogenetic and chemotaxonomic data, demonstrated that strain AH-M5T is distinguished from M. yunxiaonensis and M. flavus. On the basis of the data presented, strain AH-M5T is considered to represent a novel genus and species within the family Flavobacteriaceae, for which the name Seonamhaeicola aphaedonensis gen. nov., sp. nov. is proposed. The type strain is AH-M5T (= KCTC 32578T = CECT 8487T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2014; · 2.11 Impact Factor
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    ABSTRACT: A novel strain, designated W-15T, was isolated from the gut of the long-horned beetle, Massicus raddei, collected in South Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belonged to the suborder Micrococcineae. The strain W-15T was most closely related to Luteimicrobium album RI148-Li105T (97.9% similarity). Strain W-15T was Gram- positive, rod and coccus shaped, non-motile. Growth was observed at 15-37 °C, at pH 4.5-8.5 and in the presence of 0-5.0% NaCl. The cell-wall peptidoglycan of the strain was A4α (L-Lys-D-Ser-D-Asp). The major menaquinone present in this strain was MK-8 (H2) and the major cellular fatty acids were anteiso-C15:0, iso-C16:0, iso-C15:0 and anteiso-C17:0. The major polar lipids were diphosphatidylglycerol, phosphatidylinositol, an unknown lipid an unknown phospholipid and an unknown phosphoglycolipid. The G+C content of genomic DNA of the strain was 73.8%. On the basis of evidence from our polyphasic taxonomic study, strain W-15T is classified within a novel genus and species in the suborder Micrococcineae, for which the name Luteimicrobium xylanilyticum sp. nov. is proposed. The type strain of this genus is strain W-15T (= KCTC 19882 T = JCM 18090 T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2014; · 2.11 Impact Factor
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    ABSTRACT: A Gram-negative, aerobic, non-spore-forming, non-flagellated and rod-shaped or ovoid bacterial strain, designated SSK6-1T, was isolated from the zone where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain SSK6-1T grew optimally at 30 °C, at pH 7.0-7.5 and in the presence of 2.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences revealed that strain SSK6-1T clustered with the type strains of Phaeobacter daeponensis and Phaeobacter caeruleus, exhibiting sequence similarities of 98.6 and 98.3 %, respectively. The novel strain exhibited sequence similarities of 94.6-97.8 % to the type strains of the other Phaeobacter species and Leisingera species. Strain SSK6-1T contained Q-10 as the predominant ubiquinone and C18:1 ω7c and 11-methyl C18:1 ω7c as the major fatty acids. The major polar lipids of strain SSK6-1T were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain SSK6-1T was 64.6 mol% and its DNA-DNA relatedness values with P. daeponensis TF-218T and P. caeruleus LMG 24369T were 21 and 25 %, respectively. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain SSK6-1T is separate from other Phaeobacter species. On the basis of the data presented, strain SSK6-1T is considered to represent a novel species of the genus Phaeobacter, for which the name Phaeobacter aquaemixtae sp. nov. is proposed. The type strain is SSK6-1T (= KCTC 32538T = CECT 8399T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2014; · 2.11 Impact Factor
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    ABSTRACT: Clusterin, a protein associated with multiple functions, is expressed in a wide variety of tissues. Although clusterin is known to be involved in neurodegenerative diseases, ageing, and tumorigenesis, a detailed analysis of the consequences of gain- or loss-of-function approaches has yet to be performed to understand the underlying mechanisms of clusterin functions. Since clusterin levels change in neurological diseases, it is likely that clusterin contributes to cell death and degeneration in general. Zebrafish was investigated as a model system to study human diseases. During development, zebrafish clusterin was expressed in the notochord and nervous system. Embryonic overexpression of clusterin by mRNA microinjection did not affect axis formation, whereas its knock-down by anti-sense morpholino treatment resulted in neuronal cell death. To analyze the function of clusterin in neurodegeneration, a transgenic zebrafish was investigated, in which nitroreductase expression is regulated under the control of a neuron-specific huC promoter which is active between the stages of early neuronal precursors and mature neurons. Nitroreductase turns metronidazole into a cytotoxic agent that induces cell death within 12 h. After metronidazole treatment, transgenic zebrafish showed neuron-specific cell death. Interestingly, we also observed a dramatic induction of clusterin expression in the brain and spinal cord in these fish, suggesting a direct or indirect role of clusterin in neuronal cell death and thus, more generally, in neurodegeneration.
    Journal of Genetics and Genomics 01/2014; · 2.08 Impact Factor
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    ABSTRACT: Habitat changes of butterflies are caused by various factors, including food plants, natural enemies, and climate change. Recently, new habitats of the large copper have been reported on the Korean Peninsula, mostly along a riverside. This implies that this butterfly may move along a drainage system. In this study, new habitats of the large copper along the Geum River were investigated and the population dynamics of the butterfly were monitored in selected areas through the mark–release–recapture method. The results showed that the large copper had dispersed inland along the Geum River, which runs along the central western seashore of South Korea. In addition, the habitat distribution of the butterfly showed good coincidence with the distribution of a sorrel, Rumex japonicas, the main food plant of the large copper. A study on the moving distance of the butterfly showed that females moved further than males, while males showed more frequent movement within a short distance. The movement of the large copper along the Gum River, which lies over 100 km south of the Han River (a habitat previously reported in 1973), suggests that the movement of the butterfly might be more affected by human activities, such as the development of bike paths along the Gum River and maintenance of the riverbed, than climate change.
    Journal of Asia-Pacific Entomology 01/2014; · 0.80 Impact Factor
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    ABSTRACT: A novel facultatively anaerobic strain DH1(T) was isolated from deep sub-seafloor sediment at a depth of 900 m below the seafloor off Seo-do (the west part of Dokdo Island) in the East Sea of the Republic of Korea. The new strain was characterized using polyphasic approaches. The isolate was Gram-stain-negative, motile by gliding, non-spore-forming rods, oxidase-negative, and catalase-positive; and formed colonies of orange-red color. The NaCl range for growth was 0.5-7.0% (w/v) and no growth was observed in the absence of NaCl. The isolate grew optimally at 30°C, with 2% (w/v) NaCl and at pH 7. The cell-wall hydrolysates contained ribose as a major sugar. The DNA G+C content was 40.8 mol%. The closest related strains are Sunxiuqinia faeciviva JAM-BA0302(T) and Sunxiuqinia elliptica DQHS-4(T) (97.9 and 96.3% sequence similarity, respectively). The level of DNA-DNA relatedness between strain DH1(T) and S. faeciviva JAM-BA0302(T) was around 41% (but only 6% between DH1T and S. elliptica DQHS-4(T)). The major cellular fatty acids of the isolate were contained iso-C15:0 (25.9%), anteiso-C15:0 (16.7%), and summed feature 9 (comprising C16:0 3-OH and/or unknown fatty acid of dimethylacetal ECL 17.157; 13.2%). The predominant menaquinone was MK-7. On the basis of polyphasic evidence from this study, the isolate was considered to represent a novel species of the genus Sunxiuqinia, for which the name Sunxiuqinia dokdonensis sp. nov. is proposed; the type strain is DH1(T) (=KCTC 32503(T) =CGMCC 1.12676(T) =JCM 19380(T)).
    The Journal of Microbiology 12/2013; 51(6):741-6. · 1.28 Impact Factor
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    ABSTRACT: A Gram-staining-negative, non-motile, catalase- and oxidasepositive bacterium, designated strain I-15(T), was isolated from a crab of the Yellow Sea, Korea. On the basis of a 16S rRNA gene sequence analysis, strain I-15(T) was shown to belong to Bacteroidetes, related to the genus Aestuariibaculum. Sequence similarity between strain I-15(T) and the only type strain of the genus Aestuariibaculum, Aestuariibaculum suncheonense SC17(T), was 96.7%. Strain I-15(T) grew at 0.5-6.0% (w/v) NaCl, at 10-42°C and at pH 4.5-8.0. It could hydrolyze starch and Tweens 80. Menaquinone-6 was the only respiratory quinone, and summed features 3 (C16:1 ω7c/C16:1 ω6c) (16.4%), iso-C15:0 (15.6%), and iso-C15:1 G (12.6%) were the major cellular fatty acids. The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. The DNA G+C content was 39.0 mol%. Polyphasic data allowed genotypic and phenotypic distinction of strain I-15(T) from the only validly published Aestuariibaculum species. Therefore, the organism is considered a novel species of the genus Aestuariibaculum, for which the name Aestuariibaculum scopimerae sp. nov. is proposed. The type strain is I-15(T) (=KCTC 32459(T) =JCM 19486(T)).
    The Journal of Microbiology 12/2013; 51(6):736-40. · 1.28 Impact Factor
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    ABSTRACT: A Gram-staining-negative, catalase- and oxidase-positive bacterium, non-motile, designated strain KOPRI 21160T, was isolated from Antarctic soil. Based on 16S rRNA gene sequence analysis, strain KOPRI 21160T was found to belong to the genus Polaribacter. Sequence similarity between strain KOPRI 21160T and the type strains of Polaribacter species was 94.2-98.3%. The nearest phylogenetic neighbors of strain KOPRI 21160T were Polaribacter butkevichii KCTC 12100T (98.3%) and Polaribacter irgensii KCTC 23136T (97.5%). The DNA-DNA relatedness value between strain KOPRI 21160T and P. butkevichii KCTC 12100T was 50.6%, and 45.2% between strain KOPRI 21160T and P. irgensii KCTC 23136T. Strain KOPRI 21160T grew at 4-37°C and at pH 7.0-8.5. It could hydrolyze DNA, starch and Tweens 20, 40, 60 and 80. Menaquinone-6 (MK-6) was the only respiratory quinone, and iso-C15:0 (18.1%), iso-C15:0 3OH (17.9%) and C15:1 ω6c (10.4%) were the major cellular fatty acids. The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and one unidentified lipid. The DNA G+C content was 30.0 mol%. Based on polyphasic data, the organism is considered as a novel species of the genus Polaribacter, for which we propose the name Polaribacter sejongensis sp. nov. The type strain is KOPRI 21160T (= KCTC 23670T = JCM 18092T). Emended descriptions of the genus Polaribacter, Polaribacter butkevichii Nedashkovskaya et al. 2005 and Polaribacter irgensii Gosink et al. 1998 are also proposed.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 05/2013; · 2.11 Impact Factor
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    ABSTRACT: Zebrafish is considered as a versatile experimental animal for various research models from development to diseases. In this study, we report the development of transgenic zebrafish line named as Tg(EF1α:Kaede) that expresses translation elongation factor 1 subunit alpha (EF1α) promoter linked to a fluorescent protein (FP), Kaede for monitoring proliferating cells in during regeneration. It was revealed that about 1.4 kb 5'-flanking region of the EF1α was sufficient for its promoter activity. Expression of Kaede with a property of photo-conversion from green to red was detected in different embryonic stages as well as various organs such as brain, heart, pancreas, intestine, ovary, and fins of adult fish. Cell proliferation pattern during fin regeneration was monitored after amputation of Tg(EF1α:Kaede) caudal fin and results shown that this system is simple and efficient method for detecting proliferating cells during tissue regeneration. Developed Tg(EF1α:Kaede) line has potential to investigate the cell proliferation, regeneration, wound healing capacities after tissue damage and evaluate the therapeutic power of wound healing drugs.
    Fish &amp Shellfish Immunology 03/2013; · 2.96 Impact Factor
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    ABSTRACT: This study was conducted to investigate the biogeographic characteristics of the insect faunas of the seven islands of the west coast of Incheon, Korea, using a quantitative analysis method. The faunal similarity was examined using the Bray & Curtis similarity. The obtained similarity value matrix was examined with cluster analysis using the UPGMA method. The recorded number of each species in the areas was 1,001 from 12 orders and they were distributed on the seven islands surveyed. Among the surveyed islands, Seokmodo had the highest number of species with 497, while Yeonpyeongdo had the lowest with 136 species. The species composition of insects reported in Ganghwado was 309 species belonging to seven orders. The similarity values between the seven localities investigated ranged from 24.907 (Gyodongdo to Yeonpyeongdo) to 49.899 (Baengnyeongdo to Ganghwado). That is, the species composition of Baengnyeongdo (47.90%) was similar to that of Ganghwado, while it was different from that of Yeonpyeongdo (25.28%). The cluster analysis using a similarity index shows that all the islands in these areas can be divided into 3 groups at the level of 30.97%.
    Journal of Asia-Pacific Biodiversity. 01/2013; 6(2).
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    ABSTRACT: We report the 3.74-Mb genome sequence of Galloisinimonas intestini B14(T), isolated from the gut of one of the world's rarest insect species, Galloisiana sp., collected at a Mosan cave, Moonkyung, Gyungsangbook-do, South Korea. Strain B14(T) is a novel genus candidate of the family Enterobacteriaceae.
    Journal of bacteriology 12/2012; 194(23):6648. · 3.94 Impact Factor
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    ABSTRACT: Two novel Gram-staining-negative, motile by gliding, rod-shaped bacteria, strains N7d-4T and B4a-b5, were isolated during a study of culturable bacteria in soil cultivating potatoes. These isolates grew at 15-37 °C and pH 6.5-7.0. The major components of their cellular fatty acids were summed feature 3 (iso-C15:0 2-OH and/or C16:1 ω7c; 9.8-16.9 %), anteiso-C15:0 (13.6-16.5 %), iso-C15:0 (11.5-16.0 %), iso-C17:0 3-OH (9.6-15.1 %) and iso-C17:1 ω9c (6.9-7.7 %). The DNA G + C content of the genomic DNA was 46.9-48.5 mol% (HPLC). A phylogenetic analysis based on 16S rRNA gene sequences showed that strains N7d-4T and B4a-b5 were affiliated with Pedobacter species in the family Sphingobacteriaceae. Strains N7d-4T and B4a-b5 shared 99.9 % sequence similarity and the most closely related Pedobacter species were Pedobacter composti TR6-06T (96.5 and 96.7 %, respectively), Pedobacter oryzae N7T (95.4 and 95.6 %) and Pedobacter caeni LMG 22862T (94.0 and 94.4 %). The phenotypic and phylogenetic characteristics of the isolates allowed these two isolates to be clearly distinguished from other Pedobacter species. Based on these data, the isolates are considered to represent a novel species of the genus Pedobacter, for which the name Pedobacter luteus sp. nov. is proposed. The type species is N7d-4T (= KCTC 22699 T = DSM 22385T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 07/2012; · 2.11 Impact Factor
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    ABSTRACT: Two novel, Gram-positive, motile, coccal bacteria, strains L1b-b9(T) and B5a-b5, were isolated from a potato cultivation field in Ochang, Korea. These isolates grew at 10-45°C, pH 5.0-10.0, and in the presence of 8% (w/v) NaCl. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major menaquinone was MK-8(H(4)) and the main cellular fatty acids were iso-C(14:0), iso-C(15:0), and anteiso-C(15:0). Polar lipids in strain L1b-b9(T) consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and an unknown glyco-amino lipid. The G+C content of genomic DNA was 73.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strains L1b-b9(T) and B5a-b5 shared 99.36% similarity and formed a robust clade with the type species of the genus Phycicoccus. Strain L1b-b9(T) is related most closely to Phycicoccus cremeus V2M29(T) (97.52% 16S rRNA gene sequence similarity). On the basis of phylogenetic characteristics, the name Phycicoccus ochangensis sp. nov. is proposed for strain LIb-b9(T) (=KCTC 19694(T) =JCM 17595(T)).
    The Journal of Microbiology 04/2012; 50(2):349-53. · 1.28 Impact Factor
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    ABSTRACT: Ostrinia larvae feed the pods and stem of red bean and seriously damage the bean production from farmers. In this study we investigated biological and developmental characteristics including field collection, host feeding preference, artificial rearing diet, morphological and molecular taxonomical identification, and pheromone analysis for an Ostrinia sp. in Korea. The male adults have massive tibia in the middle legs and 3-lobed uncus in the genitalia. The partial nucleotide sequences of mitochondrial cytochrome oxidase I (COI) and II (COII) were not corresponded to those DNA sequences from other Ostrinia species reported previously in Japan and China. Host plants for this species are also different from the previous species reported. In the gas chromatography (GC) analyses, (Z)-9-tetradecenyl acetate was not detected from the pheromone gland of our species while the component as a sex pheromone was found in O. zaguliaevi and O. zealis, With taken results, we conclude this Ostrinia species in Korea is Ostrinia scapulalis or closely related species. When larvae collected in a fall were incubated in the outdoor condition, they emerged to adult between June and July in the next year. The result indicates that the winter diapause could be started in late larval stage. In addition, we developed a semi-synthetic artificial diet adopted for mass rearing of the O. scapulalis in laboratory.
    Korean journal of applied entomology. 01/2012; 51(4).
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    ABSTRACT: Strains RU-16(T), RU-28, RU-04(T) and PU-02(T) were isolated from the gut of the African mole cricket, Gryllotalpa africana. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the strains belonged to the family Microbacteriaceae. All four strains were most closely related to Curtobacterium ginsengisoli DCY26(T) (below 97 % 16S rRNA gene sequence similarity). These isolates were Gram-stain-positive, motile (by gliding), rod-shaped and exhibited ivory-coloured colonies. Their chemotaxonomic properties included MK-11 as the major respiratory quinone, ornithine as the cell-wall diamino acid, acetyl as the acyl type of the peptidoglycan, cyclohexyl-C(17 : 0) as the major fatty acid and phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids. On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, we propose a new genus in the family Microbacteriaceae, Gryllotalpicola gen. nov., with three novel species, Gryllotalpicola daejeonensis sp. nov. (type strain RU-04(T)  = KCTC 13809(T)  = JCM 17590(T)), Gryllotalpicola koreensis sp. nov. (type strain RU-16(T)  = KCTC 13810(T)  = JCM 17591(T)) and Gryllotalpicola kribbensis sp. nov. (type strain PU-02(T)  = KCTC 13808(T)  = JCM 17593(T)). Gryllotalpicola koreensis is the type species of the genus. Additionally, we propose that Curtobacterium ginsengisoli should be reclassified in the genus as Gryllotalpicola ginsengisoli comb. nov. (type strain DCY26(T)  = KCTC 13163(T)  = JCM 14773(T)).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 11/2011; 62(Pt 10):2363-70. · 2.11 Impact Factor

Publication Stats

186 Citations
76.17 Total Impact Points

Institutions

  • 2001–2014
    • Korea Research Institute of Bioscience and Biotechnology KRIBB
      • • Korea Research Institute of Bioscience and Biotechnology (KRIBB)
      • • Biological Resource Center
      • • Insect Resources Laboratory
      Anzan, Gyeonggi Province, South Korea
  • 2012
    • Iranian Biological Resource Center
      Teheran, Tehrān, Iran