Chiraz Talbi

Agence Nationale de Sécurité Sanitaire de l'Alimentation, de l'Environnement et du Travail, Île-de-France, France

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Publications (9)37.03 Total impact

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    ABSTRACT: A rhabdovirus was isolated in cell culture inoculated with tissue material from diseased grayling, Thymallus thymallus (L.), originating from a fish farm affected by a mortality episode in Poland. Diagnostics tests showed that the virus was not related to novirhabdoviruses known in Europe, nor to vesiculovirus-like species, except perch rhabdovirus (PRhV) with which it shared moderate serological relations. However, RT-PCR with PRhV probes gave negative results. To identify the virus, a random-priming sequence-independent single primer amplification was adopted. Surprisingly, two of the obtained sequences exhibited a high identity (>99%) with hirame rhabdovirus (HIRRV), a novirhabdovirus usually found in fish in marine Asiatic countries, for instance Japan, China and Korea. The full-length sequence of the phosphoprotein gene (P) demonstrated a higher identity of the present isolate with HIRRV from China compared with the Korean isolate. An identical viral sequence was also found in brown trout, Salmo trutta trutta L., affected by mortalities in a second farm in the same region, after a likely contamination from the grayling farm. To our knowledge, this is the first report of HIRRV in Europe, and in two hosts from fresh water that have not been described before as susceptible species.
    Journal of Fish Diseases 08/2013; · 1.59 Impact Factor
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    ABSTRACT: Despite the increasing impact of rhabdoviruses in European percid farming, the diversity of the viral populations is still poorly investigated. To address this issue, we sequenced the partial nucleoprotein (N) and complete glycoprotein (G) genes of nine rhabdoviruses isolated from perch (Perca fluviatilis) between 1999 and 2010, mostly from France, and analyzed six of them by immunofluorescence antibody test (IFAT). Using two rabbit antisera raised against either the reference perch rhabdovirus (PRhV) isolated in 1980 or the perch isolate R6146, two serogroups were distinguished. Meanwhile, based on partial N and complete G gene analysis, perch rhabdoviruses were divided into four genogroups, A-B-D and E, with a maximum of 32.9% divergence (G gene) between isolates. A comparison of the G amino acid sequences of isolates from the two identified serogroups revealed several variable regions that might account for antigenic differences. Comparative analysis of perch isolates with other rhabdoviruses isolated from black bass, pike-perch and pike showed some strong phylogenetic relationships, suggesting cross-host transmission. Similarly, striking genetic similarities were shown between perch rhabdoviruses and isolates from other European countries and various ecological niches, most likely reflecting the circulation of viruses through fish trade as well as putative transfers from marine to freshwater fish. Phylogenetic relationships of the newly characterized viruses were also determined within the family Rhabdoviridae. The analysis revealed a genetic cluster containing only fish viruses, including all rhabdoviruses from perch, as well as siniperca chuatsi rhabdovirus (SCRV) and eel virus X (EVEX). This cluster was distinct from the one represented by spring viraemia of carp vesiculovirus (SVCV), pike fry rhabdovirus (PFRV) and mammalian vesiculoviruses. The new genetic data provided in the present study shed light on the diversity of rhabdoviruses infecting perch in France and support the hypothesis of circulation of these viruses between other hosts and regions within Europe.
    Archives of Virology 09/2011; 156(12):2133-44. · 2.28 Impact Factor
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    ABSTRACT: [This corrects the article on p. e1001166 in vol. 6.].
    PLoS Pathogens 01/2011; 7(1). · 8.14 Impact Factor
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    ABSTRACT: Understanding the role of humans in the dispersal of predominantly animal pathogens is essential for their control. We used newly developed Bayesian phylogeographic methods to unravel the dynamics and determinants of the spread of dog rabies virus (RABV) in North Africa. Each of the countries studied exhibited largely disconnected spatial dynamics with major geopolitical boundaries acting as barriers to gene flow. Road distances proved to be better predictors of the movement of dog RABV than accessibility or raw geographical distance, with occasional long distance and rapid spread within each of these countries. Using simulations that bridge phylodynamics and spatial epidemiology, we demonstrate that the contemporary viral distribution extends beyond that expected for RABV transmission in African dog populations. These results are strongly supportive of human-mediated dispersal, and demonstrate how an integrated phylogeographic approach will turn viral genetic data into a powerful asset for characterizing, predicting, and potentially controlling the spatial spread of pathogens.
    PLoS Pathogens 01/2010; 6(10):e1001166. · 8.14 Impact Factor
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    ABSTRACT: Genetic characterization of 32 canine rabies viruses circulating in Burkina Faso in 2007 identified two clades both belonging to the Africa 2 lineage. Sequence homology data suggest that transboundary spread is the most likely means of introduction, highlighting an evolving epidemiological situation.
    Zoonoses and Public Health 12/2009; 57(7-8):e42-6. · 2.09 Impact Factor
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    ABSTRACT: The burden of rabies in Africa is estimated at 24,000 human deaths year(-1), almost all of which result from infection with dog rabies viruses (RABV). To investigate the evolutionary dynamics of RABV in western and central Africa, 92 isolates sampled from 27 African countries over 29 years were collected and sequenced. This revealed that RABV currently circulating in dogs in this region fell into a single lineage designated 'Africa 2'. A detailed analysis of the phylogeographical structure of this Africa 2 lineage revealed strong population subdivision at the country level, with only limited movement of virus among localities, including a possible east-to-west spread across Africa. In addition, Bayesian coalescent analysis suggested that the Africa 2 lineage was introduced into this region of Africa only recently (probably <200 years ago), in accordance with the timescale of expanding European colonial influence and urbanization, and then spread relatively slowly, perhaps occupying the entire region in a 100 year period.
    Journal of General Virology 04/2009; 90(Pt 4):783-91. · 3.13 Impact Factor
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    ABSTRACT: The matrix (M) proteins of rhabdoviruses are multifunctional proteins essential for virus maturation and budding that also regulate the expression of viral and host proteins. We have solved the structures of M from the vesicular stomatitis virus serotype New Jersey (genus: Vesiculovirus) and from Lagos bat virus (genus: Lyssavirus), revealing that both share a common fold despite sharing no identifiable sequence homology. Strikingly, in both structures a stretch of residues from the otherwise-disordered N terminus of a crystallographically adjacent molecule is observed binding to a hydrophobic cavity on the surface of the protein, thereby forming non-covalent linear polymers of M in the crystals. While the overall topology of the interaction is conserved between the two structures, the molecular details of the interactions are completely different. The observed interactions provide a compelling model for the flexible self-assembly of the matrix protein during virion morphogenesis and may also modulate interactions with host proteins.
    PLoS Pathogens 01/2009; 4(12):e1000251. · 8.14 Impact Factor
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    ABSTRACT: Lyssaviruses are RNA viruses with single-strand, negative-sense genomes responsible for rabies-like diseases in mammals. To date, genomic and evolutionary studies have most often utilized partial genome sequences, particularly of the nucleoprotein and glycoprotein genes, with little consideration of genome-scale evolution. Herein, we report the first genomic and evolutionary analysis using complete genome sequences of all recognised lyssavirus genotypes, including 14 new complete genomes of field isolates from 6 genotypes and one genotype that is completely sequenced for the first time. In doing so we significantly increase the extent of genome sequence data available for these important viruses. Our analysis of these genome sequence data reveals that all lyssaviruses have the same genomic organization. A phylogenetic analysis reveals strong geographical structuring, with the greatest genetic diversity in Africa, and an independent origin for the two known genotypes that infect European bats. We also suggest that multiple genotypes may exist within the diversity of viruses currently classified as 'Lagos Bat'. In sum, we show that rigorous phylogenetic techniques based on full length genome sequence provide the best discriminatory power for genotype classification within the lyssaviruses.
    PLoS ONE 02/2008; 3(4):e2057. · 3.53 Impact Factor
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    ABSTRACT: Introduction Hirame rhabdovirus (HIRRV) is one of the four recognized species within the Novirhabdovirus genus, represented by the type species Infectious Haematopoietic Necrosis (IHNV). HIRRV was first isolated during an outbreak on cultured flounder (Paralichthys olivaceus) and ayu (Plecoglossus altivelis) in Japan (1). It was also found on other marine fish in Asia, such as stone flounder (Kareius bicoloratus) in China (2). Furthermore, it was shown to be pathogenic for a range of salmonids species, including rainbow trout, experimentally challenged in freshwater. The major clinical signs of HIRRV infection were congestion of the gonads, focal hemorrhages of the skeletal muscle and fins and ascitic fluid collection (3). We report the first description of HIRRV in Europe, isolated from grayling and brown trout in a farm in Poland. Materials & methods Thirty adult graylings (Thymallus thymallus) with clinical signs and thirty asymptomatic adult brown trouts (Salmo trutta m. fario) from the same farm in Poland were tested for the presence of novirhabdoviruses by cell culture. Pools of kidney and spleen from a maximum of 10 fish were homogenized. For virus propagation, epithelioma papulosum cyprini (EPC), fathead minnow (FHM), rainbow trout gonad (RTG) and bluegill fry (BF-2) cell lines were inoculated and incubated at 15°C. Cell cultures were collected for virus identification when cytopathic effect (CPE) appeared, usually 4 to 7 d later. Starting from RNA extracted from cell culture supernatant, a random-priming sequence-independent single primer amplification (SISPA) was adopted to search for viral sequences (4). PCR products were cloned and sequenced according to the Sanger method.

Publication Stats

171 Citations
37.03 Total Impact Points


  • 2011
    • Agence Nationale de Sécurité Sanitaire de l'Alimentation, de l'Environnement et du Travail
      Île-de-France, France
  • 2009
    • Institut Pasteur
      Lutetia Parisorum, Île-de-France, France
    • University of Oxford
      • Wellcome Trust Centre for Human Genetics
      Oxford, ENG, United Kingdom