[Show abstract][Hide abstract] ABSTRACT: Non-obstructive azoospermia is the most challenging type of male infertility. Stem cell based therapy provides the potential to enhance the recovery of spermatogenesis following cancer therapy. Bone marrow-derived mesenchymal stem cells (BMSCs) possess the potential to differentiate or trans-differentiate into multi-lineage cells, secrete paracrine factors to recruit the resident stem cells to participate in tissue regeneration, or fuse with the local cells in the affected region. In this study, we tested whether spermatogenically-induced BMSCs can restore spermatogenesis after administration of an anticancer drug. Allogeneic BMSCs were co-cultured in conditioned media derived from cultured testicular Sertoli cells in vitro, and then induced stem cells were transplanted into the seminiferous tubules of a busulfan-induced azoospermatic rat model for 8 weeks. The in vitro induced BMSCs exhibited specific spermatogonic gene and protein markers, and after implantation the donor cells survived and located at the basement membranes of the recipient seminiferous tubules, in accordance with what are considered the unique biological characteristics of spermatogenic stem cells. Molecular markers of spermatogonial stem cells and spermatogonia (Vasa, Stella, SMAD1, Dazl, GCNF, HSP90α, integrinβ1, and c-kit) were expressed in the recipient testis tissue. No tumor mass, immune response, or inflammatory reaction developed. In conclusion, BMSCs might provide the potential to trans-differentiate into spermatogenic-like-cells, enhancing endogenous fertility recovery. The present study indicates that BMSCs might offer alternative treatment for the patients with azoospermatic infertility after cancer chemotherapy.
International Journal of Molecular Sciences 01/2014; 15(8):13151-65. · 2.46 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: To investigate the expression of c-Jun N-terminal kinase (JNK) 1/2 in human hypospadiac tissue compared with that in normal penile tissue.
Penile skin tissue specimens obtained at surgery during hypospadias repair or during elective circumcision were divided into 3 groups according to the position of the urethral meatus: children with mild hypospadias (n = 16), severe hypospadias (n = 16), and normal controls (n = 16). The expression of total and phosphorylated JNK1 and JNK2 at the messenger ribonucleic acid and protein levels were assessed using real-time quantitative polymerase chain reaction, immunochemistry, and Western blot analysis.
JNK1 messenger ribonucleic acid expression and JNK1 and JNK2 phosphorylated protein levels increased significantly in subjects with mild or severe hypospadias compared with the controls (P <.05). JNK2 phosphorylated protein levels increased significantly in those with severe hypospadias compared with those with mild hypospadias (P <.05). Those with hypospadias had increased phosphorylation protein expression of JNK1/2 in the mesenchymal cell layers of the preputial subcutaneous mesenchymal cell layer.
Our findings suggest that JNK upregulation might contribute to the development of hypospadias and might associated with mesenchymal cell migration in the process of external male genitalia defect development.
[Show abstract][Hide abstract] ABSTRACT: Ureteropelvic junction obstruction (UPJO) is the most common congenital anomaly of the urinary tract. Evidence has shown that BMP4 and Id2 play crucial roles in nephrogenesis, alterations of which may cause ureteral developmental anomalies. Here, we directly sequenced the coding sequences in BMP4 and Id2 genes of 108 unrelated Chinese patients with ureteropelvic junction stenosis. One missense mutation c.485G> A (p.R162Q) in BMP4 and two synonymous mutations (c.1167T> C in BMP4 and c.108A> G in Id2) were detected in three cases. None of these variations were present in the 150 normal controls. Comparative amino acid sequence alignments of BMP4 in humans and other vertebrate orthologs show that p.R162 located to a highly conserved amino acid residue. Moreover, computational analysis predicted that R162Q probably infect the function of BMP4 protein. CONCLUSION: The mutation c.485G> A in BMP4 might be one of the causes of human UPJO. Further functional studies are required to validate the association between this variation and UPJO. Otherwise, Id2 mutations do not seem to be involved in this disease.
European Journal of Pediatrics 09/2011; 171(3):451-6. · 1.98 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: We describe laparoscopic management of multiple ureteral polyps and our objective initial experience in children.
We used laparoscopic polypectomy and pyeloureterostomy to treat 13 patients (15 ureters) diagnosed with hydronephrosis caused by multiple ureteral polyps between August 2006 and November 2010. Mean patient age was 11.4 years. The polyps were left sided in 9 patients, right sided in 2 and bilateral in 2. Hydronephrosis was mild in 9 cases, moderate in 4 and severe in 2. Postoperative followup consisted of ultrasound and excretory urography.
A total of 12 patients were successfully treated with laparoscopy, while 1 patient with bilateral polyps required conversion to open surgery. Mean polyp length was 4.5 cm. Mean operative time in the unilateral cases was 121 minutes. The bilateral polyps were operated on simultaneously, with a surgical time of 185 minutes. Mean hospital stay was 3.5 days. Followup ranged from 6 to 26 months. In 1 patient obstruction of the temporary Double-J® stent developed 1 week postoperatively due to the deposition of urinary salts. No recurrent polyps were seen on followup B-mode ultrasound or excretory urography.
Laparoscopic management of multiple ureteral polyps in children can be considered a safe and effective minimally invasive surgical option. However, further studies with larger cohorts are needed to confirm this finding.
The Journal of urology 08/2011; 186(4):1444-9. · 3.75 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Kidney ischemia-reperfusion injury (IRI) after kidney transplant remains a major problem, separate from immune rejection that can lead to kidney transplant failure and graft function loss. Free radicals, disturbance of microcirculation and the inflammatory cascade appear to be the main contributors. Radix Codonopsis, a traditional Chinese drug used in vascular diseases, is an antioxidant and free radical scavenger. This study investigates the protective effect and underlying mechanisms of Radix Codonopsis extract saponins on kidney transplantation.
Renal transplantation was performed after rat kidneys had been stored for 1 h at 4°C. Blood urea nitrogen (BUN), serum creatinine (Scr), superoxide dismutase (SOD) and malondialdehyde (MDA) were assayed; bcl-2 and bax mRNA expression was detected using RT-PCR; bcl-2 and bax protein expression was detected by immunohistochemistry (IHC). Terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) was used to detect apoptotic cells and determine the apoptosis index (AI). Analysis of variance (ANOVA) followed by Dunnett's test was used when more than two groups were compared.
Saponin-treated animals showed increased SOD levels accompanied by decreased MDA, Scr and BUN levels (p < 0.05 vs. untreated controls); bcl-2 mRNA and protein levels were increased in transplanted kidney from treated animals, while bax mRNA and protein levels were decreased (p < 0.05 vs. untreated controls). AI was significantly decreased in transplanted kidneys from treated animals relative to untreated controls (p < 0.05 vs. untreated controls).
This study clearly demonstrates the protective effects on IRI after kidney transplantation, which may be explained by decreased lipid peroxidation and inhibition of apoptosis.
Pediatric Surgery International 06/2011; 27(11):1203-12. · 1.22 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Lipoma of the kidney is a rare benign renal tumor that previously had been reported only in adults. We present a case of this tumor in a 2-year-old boy. Serial imaging studies revealed a tumor with high fat content arising from the right kidney. Radical nephrectomy was performed for tumor excision. Macroscopically, the tumor consisted of a yellow fatty mass, which was surrounded by a thin fibrous capsule. The tumor was about 600 g, and the kidney was compressed laterally by the tumor. Microscopically, the tumor consisted of large fat cells that did not express human melanoma black-45 (HMB-45). It was diagnosed as a renal lipoma. Postoperatively, there has been no recurrence of tumor in the 10 months since the surgical excision.
Journal of Pediatric Surgery 06/2011; 46(6):1281-3. · 1.38 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: The aim of this study is to explore the mechanism by which acrolein (ACR), a metabolite of cyclophosphamide (CP), induces immature Sertoli cell cytoskeletal changes. Sertoli cells obtained from rats were cultivated and treated with 50 and 100 μM ACR. XTT assays were performed to detect cell viability. Activities of superoxide dismutase (SOD), glutathione peroxidases (GSH-Px), and catalase (CAT), as well as total anti-oxidation competence (T-AOC) were examined. Superoxide anion levels were detected by a fluorescent probe. Cell ultrastructure changes were observed by transmission fluorescent microscope. Actin filament (F-actin) distribution was detected by immunofluorescence, and ERK and p38MAPK expression were detected by western blot analysis. ACR significantly decreased the viability of Sertoli cells in a dose- and time-dependent manner. T-AOC and the antioxidant activity of SOD, CAT and GSH-Px, were decreased in ACR-treated groups compared with the control group. The levels of reactive oxygen species (ROS) in ACR-treated Sertoli cells were increased. In addition, characteristics of cell apoptosis such as mitochondrial swelling, aggregated chromatin, condensed cytoplasm, nuclei splitting, and nuclei vacuolization were observed in ACR-treated cells. Furthermore, ACR-treatment also induced microfilament aggregation, marginalization and regionalization. The expression levels of ERK and p38MAPK were also increased in ACR-treated cells in a dose- and time-dependent manner. ACR, a major CP metabolite, impairs the cytoskeleton which is likely caused by induction of the oxidative stress response through up-regulation of ERK and p38MAPK expression.
[Show abstract][Hide abstract] ABSTRACT: The study aimed to investigate the method of management and diagnosis of pediatric blunt renal trauma, and determine whether all grades of hemodynamically stable injuries can be managed conservatively.
Eighty-four children, presented with blunt renal trauma and treated in the urinary surgery department of Children's Hospital of Chongqing Medical University, were reviewed. Data collected from the medical records of the identified patients included: (1) demographics, (2) management, (3) severity of hematuria, (4) findings on computed tomography, (5) associated injuries, (6) duration of hospital stay, and (7) follow-up complications.
Of the 84 patients, only 11 (13%) required operations. Five (6%) patients were immediately operated for vascular instability; one (1.2%) patient had delayed operation for missed Ureteropelvic Junction (UPJ) disruption, one (1.2%) patient for abscess, and four (4.7%) patients for persistent bleeding. All the children with low-grade injuries and part of them with high-grade injuries were selected for nonoperative management of real injuries. The diagnosis rate of blunt renal trauma using Ultrasonography (US) was 91%. The rate of diagnosis by intravenous pyelography (IVP) was 82% and that by computed tomographic (CT) was 100%. However, Ultrasonography could not diagnose all patients with grade I injuries. Only 3 (8%) of 36 children had hypertension managed conservative by followed-up for 4 months.
The goals of treatment of blunt renal injuries include minimizing accrued staging and complications. Our study supported that conservative management is the first choice for all grades of hemodynamically stable children with blunt renal trauma. Abdominal CT scanning is the most accurate screening test for high-grade injuries and older children.
International Urology and Nephrology 04/2011; 43(4):937-42. · 1.33 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: This study aimed to compare clinical manifestations, physical examination findings, laboratory, and radiographic dates in pediatric patients with different pathological diagnoses of acute scrotum as well as to accurately establish the true incidence of various pathological diagnoses of acute scrotum.
The records of children hospitalized for acute scrotum from 1990 to 2008 were reviewed.
A total of 1228 cases were included in the study (103 with testicular torsion, 918 with torsion of the testicular appendix, 72 with tunica vaginalis inflammation, 46 with epididymitis and 89 with other pathological diagnoses). Duration of pain less than 6 hours, fever, vomiting, history of trauma or activities, absence of cremasteric reflex, and abnormal testicle direction were significantly associated with testicular torsion. Blue dot sign and tender nodule were found significantly associated with torsion of the testicular appendix. Ultrasound showed decreased or absent blood flow in 91.3% testicular torsion patients; enlarged epididymis was found in 91.1% and 91.3% patients with torsion of the testicular appendix and epididymitis, respectively; and scrotal wall edema and hydrocele were found significantly associated with tunica vaginalis inflammation. Our salvageability rate in testicular torsion was 30.1%.
Overlap existed between testicular torsion and other acute scrotum. The clinical manifestations, physical examination findings, laboratory, and radiographic data were helpful in distinguishing acute scrotum. Doppler ultrasound is an indispensable imaging modality for the clinical assessment of patients with acute scrotum; in the presence of a clinical suspicion of testicular torsion, even with an apparently normal-color Doppler ultrasound, surgical exploration is still indicated.
Pediatric emergency care 04/2011; 27(4):270-4. · 0.92 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: To investigate the relationship between nitric oxide synthase (NOS) and oxidative stress and pathologic remodeling in the partial obstructed bladder of a rat model.
We surgically established partial bladder outlet obstruction (PBOO) in 2 groups of rats and allowed it to persist for 3-6 weeks. Normal and sham-operated rats served as the controls. Each group contained 6 rats for a total of 24 rats. Cystometry was used to evaluate the bladder function. The bladders were removed, and histopathologic measurements were performed to evaluate bladder hypertrophy and fibrosis and NOS immunolocalization. Biochemical measurements were used to evaluated NOS mRNA and activity and the oxidative stress level.
The obstructed rats experienced significant increases in bladder weight, muscle hypertrophy, and deposits of collagen fibers compared with the normal and sham-operated groups. PBOO debilitated bladder contractibility, increased the residual urine volume and voiding interval, decreased the voiding volume, and caused poor bladder emptying, with an increased residual urine volume and decompensated bladder in the PBOO rats at 6 weeks. The elevation in malondialdehyde and reduction in superoxide dismutase activity in the PBOO rats suggested that oxidative stress injury occurred in the obstructed bladder. Lower inducible NOS and endothelial NOS (eNOS) mRNA expression was demonstrated through quantitative polymerase chain reaction. In particular, eNOS was significantly downregulated in the PBOO rats compared with the normal and sham-operated rats. The normal and PBOO bladder tissues did not express detectable levels of neuronal NOS mRNA or exhibit neuronal NOS immunoreactivity. The total NOS activity had decreased progressively in the PBOO groups in conjunction with the significantly decreased eNOS activity compared with that in the normal and sham-operated groups.
These findings suggest that decreases in NOS activity and expression (mainly of eNOS) concomitant with increases in reactive oxygen species generation represent the underlying pathogenic mechanism of obstructed bladder remodeling and dysfunction.
[Show abstract][Hide abstract] ABSTRACT: MDR1 gene encoding P-glycoprotein is an ATP-dependent drug efflux transporter and related to drug resistance of yolk sac carcinoma. Ultrasound microbubble-mediated delivery has been used as a novel and effective gene delivery method. We hypothesize that small interfering RNA (siRNA) targeting MDR1 gene (siMDR1) delivery with microbubble and ultrasound can down-regulate MDR1 expression and improve responsiveness to chemotherapeutic drugs for yolk sac carcinoma in vitro.
Retroviral knockdown vector pSEB-siMDR1s containing specific siRNA sites targeting rat MDR1 coding region were constructed and sequence verified. The resultant pSEB-siMDR1 plasmids DNA were encapsulated with lipid microbubble and the DNA release were triggered by ultrasound when added to culture cells. GFP positive cells were counted by flow cytometry to determine transfection efficiency. Quantitative real-time PCR and western blot were performed to determine the mRNA and protein expression of MDR1. P-glycoprotein function and drug sensitivity were analyzed by Daunorubicin accumulation and MTT assays.
Transfection efficiency of pSEB-siMDR1 DNA was significantly increased by ultrasound microbubble-mediated delivery in rat yolk sac carcinoma L2 (L2-RYC) cells. Ultrasound microbubble-mediated siMDR1s delivery effectively inhibited MDR1 expression at both mRNA and protein levels and decreased P-glycoprotein function. Silencing MDR1 led to decreased cell viability and IC50 of Vincristine and Dactinomycin.
Our results demonstrated that ultrasound microbubble-mediated delivery of MDR1 siRNA was safe and effective in L2-RYC cells. MDR1 silencing led to decreased P-glycoprotein activity and drug resistance of L2-RYC cells, which may be explored as a novel approach of combined gene and chemotherapy for yolk sac carcinoma.
Journal of Experimental & Clinical Cancer Research 01/2011; 30:104. · 3.07 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: A broad spectrum of scrotal pathologies, including tumors, malformations, and inflammation, can present as painless scrotal masses in the pediatric age groups. The aim of our single institutional retrospective study was to survey data regarding uncommon painless scrotal masses collected over the past 22 years in order to better diagnose and treat these pathologies.
This retrospective study included children referred to our hospital because of scrotal masses between October 1986 and May 2009. In all cases, age at diagnosis, history, and physical examination details, biochemical markers, and findings of imaging were collected and analyzed. Definite diagnoses of the causes of the scrotal masses were made through histopathological examination.
A total of 161 children were examined because of scrotal masses during the study period. Their ages ranged from 1 month and 2 days to 14 years and 9 months (median, 2 years 11 months). Bilateral scrotal masses were observed in 17 cases. Of 161 scrotal masses, 119 (73.9%) originated from testes, 27 (16.8%) from epididymis, and 15 (9.3%) from the scrotal wall. Of these, 116 (72%) were diagnosed as tumors, 22 (13.7%) as malformations, and 23 (14.3%) as inflammation. Fourteen patients had misdiagnosed histories; of these, the majority (11) were diagnosed with yolk-sac tumors.
Although testicular tumors are the major causes of pediatric painless scrotal masses, other causes are possible. Patient history and physical examination can provide some clues as to the source of the mass. Ultrasound, alpha-fetoprotein levels, and biochemical analysis have a significant role in the evaluation of uncommon pediatric painless scrotal masses.
International Urology and Nephrology 12/2010; 42(4):979-84. · 1.33 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Wilms' tumor is the most common malignant solid tumor of the kidneys in children. Extrarenal Wilms' tumor is extremely rare. Herein, we report an 8-month-old boy with a chief complaint of frequent micturition and dysuria for 10 days. Physical examination and ultrasonography evaluation revealed simultaneous involvement of neoplasms in the left kidney and the bladder. Following excision of the masses, both were identified as Wilms' tumor by histopathology and immunohistochemistry. The two neoplasms are presumed to have developed independently because of the different pathological manifestation.
Pediatric Surgery International 03/2010; 26(3):319-22. · 1.22 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: This study investigated epigenetic (specifically, DNA methylation) changes and their impact on gene expression in testes induced by maternal exposure to Di-2-(ethylhexyl) phthalate (DEHP) in mice. Testicular dysgenesis syndrome was induced in fetuses and pups by maternal exposure to DEHP at 500 mg/kg/d, and testes were excised for analysis on gestation day (GD) 19 and postnatal days (PNDs) 3, 21, 56, and 90. High-performance liquid chromatography (HPLC) was performed to analyze DNA methylation status, and expression levels of the DNA methyltransferases were examined by quantitative real-time polymerase chain reaction (qPCR). Testis-specific gene, insulin-like hormone 3 (Insl3), and testosterone production were also detected. DEHP significantly increased DNA methylation levels on GD 19 and PND 3 (P < .05 and P < .05) but not on PNDs 21, 56, and 90. DEHP also significantly increased the expression of DNA methyltransferases. For DNA methyltransferase 1, the difference was not significant on PND 21, and DNA methyltransferase 3a and 3b returned to normal levels on PND 56. Fetal testes were a main target for DEHP as evidenced by a reduction in Insl3 expression and testosterone production. Effects of DEHP on Insl3 expression continued until PND 21. The DEHP-induced suppression of testosterone had not recovered on PND 56. Changes in DNA methylation may play an important role in abnormal testicular function caused by environmental factors such as maternal exposure to DEHP, which may be a mechanism of DEHP-mediated testicular toxicity.
International Journal of Toxicology 01/2010; 29(2):193-200. · 1.35 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: The aim of this study was to analyse epigenetic (specifically, DNA methylation) change in testes induced by maternal exposure to di-2-(ethylhexyl) phthalate (DEHP) in mice. Testicular dysgenesis syndrome was induced in foetuses by maternal exposure to DEHP. High-performance liquid chromatography was performed to analyse DNA methylation status, and expression levels of the DNA methyltransferases were examined by quantitative real-time polymerase chain reaction and western blotting. DEHP significantly had more than 10% relative increase in the global DNA methylation and also increased DNA methyltransferases' expression. Changes in DNA methylation may play an important role in abnormal testicular function caused by environmental factors such as maternal exposure to DEHP, which may be one possible mechanism of DEHP-mediated testicular toxicity.
[Show abstract][Hide abstract] ABSTRACT: To investigate the effect of di-(2-ethylhexyl) phthalate (DEHP) on the expression of activating transcription factor 3 (ATF3) and apoptosis of fetal mouse genital tubercle (GT).
In this developmental toxicity study, pregnant C57BL/6 mice were exposed to corn oil or DEHP (100 or 500 mg/kg/day) from embryonic day 12 (ED12) to ED16. Apoptosis was characterized by Terminal transferase dUTP nick end labeling (TUNEL) assay. Using RT-PCR and western blot, the expressions of ATF3 and apoptosis-related genes (P53, Bcl-2 and Bax) were investigated.
Apoptosis of fetal mouse GT cells notably decreased after DEHP treatment. DEHP activated ATF3 both at the mRNA and protein levels in GT. Furthermore, pro-apoptotic P53 was downregulated and the ratio of anti-apoptotic (Bcl-2)/pro-apoptotic (Bax) was not significantly changed.
These results suggest that DEHP may induce external genital defects via a mechanism involving apoptosis, which might correlate with the regulation of ATF3 and P53 expressions.
Journal of Occupational Health 01/2009; 51(1):57-63. · 1.63 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: To observe the hemodynamic and histological effects of unilateral testicular torsion on the contralateral testis in immature rats, and compare the results of different treatments.
Testicular torsion models were established in 3-week-old rats and randomized into a normal control, a testicular torsion, a reposition and an orchiectomy group. The systolic peak velocity of the right testicular artery was measured by color Doppler before and 8, 12, 24 and 72 h after the operation. Histological observations of the right testes were performed 2 h after testicular torsion, 12 h after testicular reposition and orchiectomy and when the rats were 9 weeks old.
The blood supply of the immature right testes increased continuously after testicular torsion of the left side. Interstitial edema and ultrastructure changes were observed in the testicular torsion, reposition and orchiectomy groups. The right testis weight was significantly greater in both the testicular torsion and orchiectomy groups than in the normal control group of the 9-week-old rats (P < 0.01). No significant differences were noted in the right testicular seminiferous tubule diameter (STD) , count measure spermatogenic (CMSE) and testicular biopsy score (TBS) among the four groups (P > 0.05).
Unilateral testicular torsion increases blood supply and induces histological changes in the contralateral testis in immature rats. Reposition and orchiectomy following light injury are prognostic of similar results.
Zhonghua nan ke xue = National journal of andrology 09/2008; 14(9):815-8.
[Show abstract][Hide abstract] ABSTRACT: This study investigated the safety and efficacy of using the minimally invasive operation of laparoscopic orchiopexy to treat palpable inguinal canalicular undescended testes.
Laparoscopic orchiopexy was performed on 90 Chinese patients (103 testes) with inguinal canalicular palpable undescended testes. Patients with testes that were nonpalpable, ectopic, retractable, or palpable but distal to the external ring were excluded. Patients in the study ranged from 8 months to 6 years old (mean age: 17 months). Of the 103 testes undergoing operation, 26.7% were on the left side of the body and 58.9% were on the right; 14.4% of the patients had bilateral undescended testes. Laparoscopic techniques were used by the same surgeon to move the spermatic vessels and transfer the testis into the scrotum.
All 103 testes were successfully descended by laparoscopy. In the first 46 unilateral cases, the operation took significantly longer for the first 15 patients than the next 31, which were completed in 32.7 +/- 5.2 min. Of all 90 patients, a complication was encountered in only one, and this occurred at the beginning of the surgeon's learning curve. Of 77 unilateral undescended testes, a patent processes vaginalis was found in 90.3% of the cases on the ipsilateral side and 15.6% on the contralateral side. All of the testes maintained an adequate size and intrascrotal position with no atrophy or recurrent inguinal hernia during a follow-up of 6 to 12 months.
The laparoscopic approach to orchiopexy is a safe way to descend the inguinal canalicular palpable testis. It offers several advantages of a minimally invasive technique and yield effective results.
Journal of endourology / Endourological Society 08/2008; 22(8):1745-9. · 1.75 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: To explore the protective effect of the Phyllanthus Urinaria (PU) extract on the N-cadherin expression in the testicular tissues disrupted by nitrogen mustard (HN2) in vivo.
HN2 was intraperitoneally injected into male KM mice at the dose of 5 mg/kg to make reproductive toxicity models, and at the same time PU was administered for intervention at the dose of 125 mg/kg, 250 mg/kg and 500 mg/kg. N-cadherin distribution, mRNA and protein expression in the testicular tissues were detected by immunohistochemistry, RT-PCR and Western blotting.
N-cadherin was mainly distributed in the membrane and cytoplasm of Sertoli cells at the basement of seminiferous epithelia, Leydig cells and peritubular cells, scarcely expressed in the basement of seminiferous epithelia and peritubular cells after HN2 administration. The expressions of mRNA and proteins of N-cadherin were significantly elevated with the increased dose of PU (P < 0.01). Compared with the normal control, the distribution and expression of N-cadherin showed no significant differences in either the high-dose PU group or the HN2 with high-dose PU intervention group (P > 0.05).
The PU extract can effectively promote the N-cadherin expression in the testis tissues disrupted by HN2.
Zhonghua nan ke xue = National journal of andrology 06/2008; 14(5):396-400.