ABSTRACT: To evaluate the efficacy of Pleurotus ostreatus extract in preventing selenite-induced cataractogenesis.
In vitro, enucleated rat lenses, divided into one control and three experimental groups (selenite only, simultaneous selenite and extract, initial extract and subsequent selenite), underwent morphological and biochemical evaluation. The anti-cataractogenic effect was also evaluated in vivo.
In vitro, simultaneous incubation of extract with selenite-challenged lenses caused a decrease in lens opacification by maintaining antioxidant components at near normal levels. In vivo, P.ostreatus (300 mg/kg body weight) prevented cataract in 75% of rats.
Extract of P. ostreatus prevents experimental selenite-induced cataractogenesis.
Current eye research 05/2009; 34(4):264-73. · 1.51 Impact Factor
ABSTRACT: The present study sought to evaluate the efficacy of the naturally-occurring polyphenol, ellagic acid, in preventing selenite-induced cataractogenesis. In the present study, Wistar rat pups were divided into 3 groups of 15 each. Group I (normal) rats received an intraperitoneal (i.p.) injection of normal saline on postpartum day 10; group II (cataract-untreated) rats received a single subcutaneous (s.c.) injection of sodium selenite (19 micromol/kg body weight) on postpartum day 10; group III (cataract-treated) pups received a single s.c. injection of sodium selenite on postpartum day 10 and intraperitoneal injections of ellagic acid (200mg/kg body weight) on postpartum days 9-14. At the end of the study period (30th postpartum day), slit-lamp examination of both eyes of each rat pup revealed no lenticular opacification (cataract stage 0) in all eyes of group I pups, definite nuclear cataracts (stages 4-6) in the eyes of all (100%) group II rat pups and no lenticular opacification in eight (53%) and mild lenticular opacification (cataract stages 1-3) in seven (47%) of group III rats (changes in group II vs group III, P<0.01). The mean activities of the antioxidant enzymes catalase, glutathione peroxidase, superoxide dismutase and glutathione-S-transferase were significantly lower in lenses of Group II rats than in Group I or Group III rat lenses. In addition, the mean levels of GSH in lenses and erythrocytes were also significantly lower in Group II rats than in Group I or Group III rats. Conversely, the mean concentration of MDA (an indicator of lipid peroxidation) in lenses and erythrocytes was found to be significantly higher in Group II rats than that in Group I or Group III rats. Also, the mean concentration of calcium was found to be significantly higher in lenses of Group II rats than in those of Group I and Group III rats. The results suggest that ellagic acid can prevent or retard experimental selenite-induced cataractogenesis in Wistar rats. This protective effect in rat lenses appears to occur by maintaining the antioxidant defense system and inhibition of lipid peroxidation.
Experimental Eye Research 03/2008; 86(2):251-9. · 3.26 Impact Factor
ABSTRACT: To investigate whether acetyl-L-carnitine (ALCAR) retards selenite-induced cataractogenesis in vivo.
On postpartum day 10, group I pups received intraperitoneal saline and group II and group III pups received subcutaneous sodium selenite; Group III pups also received intraperitoneal ALCAR once daily on postpartum days 9-14. Both eyes of each pup were examined up to postpartum day 30. After sacrifice, extricated pup lenses were analyzed for antioxidant and redox system components.
There was dense lenticular opacification in all group II pups, minimal opacification in 33% of group III pups, and no opacification in 67% of group III and in all group I pups. Group II lenses exhibited significantly lower values of antioxidant and redox system components and higher malondialdehyde concentrations than group I or group III lenses.
ALCAR prevents selenite-induced cataractogenesis in Wistar rat pups, possibly by inhibiting depletion of antioxidant enzyme and redox system components and inhibiting lipid peroxidation.
Current Eye Research 12/2007; 32(11):961-71. · 1.28 Impact Factor