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ABSTRACT: Summary The precipitins gradually appearing in the sera of rabbits submitted to various immunization techniques were carefully localized in immunoelectrophoregrams. One precipitin is characterized by its precocity as well as its constancy. The antigenic component that corresponds to it is major and specific. The reproductibility of the immunoelectrophoregrams are excellent when revealed by a hyperimmuneserum prepared under good and standardized conditions. The sera of the rabbit immunized by intravenous injections produce a very thick cathodic band the significance of which is not entirely ascertained.
07/2009; 4(3):148-157.
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ABSTRACT: Summary Immunoelectrophoretic and enzymatic studies of Aspergillus flavus, A. effusus and other pathogenic spp. show that the antigenicity of A. flavus is indistinguishable from that of A. effusus apart from the presence of a malicodehydrogenase activity in A. effusus. There was a feeble antigenic reltionship between A. flavus and A. fumigatus, A. restrictus and A. nidulans. The antigenic fractions of A. flavus detectable by the order of appearance of serum precipitins in experimental rabbits, were established. The specificity of enzyme-antienzyme complexes was verified by the use of heterologous antigenic extracts. The exposure of fungal, bacterial and helminthological antigens to serum precipitins of patients, confirmed the specificity of 2 A. flavus antigens viz: chymotrypsin and malicodehydrogenase. This may prove of diagnostic significance.
07/2009; 9(3):210-220.
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ABSTRACT: Summary Agar gel electrophoresis of soluble extracts obtained from 9 cultures of Paracoccidioides brasiliensis, in alkaline pH, revealed 8 protein fractions, 5 aniodic and 3 cathiodic. Electrophoregrams revealed 15 enzymes, only 6 of which were further identified on immunoelectrophoregrams. Immunoelectrophoretic analysis of the extracts with hyperimmune serum prepared in rabbits confirmed that P. brasiliensis has a minimum of 25 antigenic fractions. One of them of cathodic mobility with alkaline phosphatase activity, reacted with all immune sera employed and thereby produced a characteristic antigen antibody system. An antibody corresponding to this fraction was formed early during the experimental infection of rabbits. This suggested that the antigen involved in the formation of arc E, was specific in human paracoccidioidomycosis. The existence of various antigenic fractions common to P. brasiliensis and species of Emmonsia was verified.
07/2009; 11(1):80-88.
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ABSTRACT: Serum samples from patients with candidosis and from rabbits experimentally infected with serotype B C. albicans strains consistently showed higher antibody titers against Candida strains with serotype A antigens than strains with serotype B antigens, in indirect fluorescent antibody tests. When sera from rabbits infected with C. albicans serotype B strains were absorbed with blastospores of the homologous strain they continued to react against strains of C. albicans serotype A and a C. tropicalis strain with serotype A antigens. Serotype A-specific antisera reacted against tissue forms of C. albicans serotype B in vivo and against serotype B germ tubes, but not their parent blastospores, in vitro. These findings suggest that C. albicans B serotype cells may sometimes express, in vitro or in vivo, one or several antigens so far considered as specific of serotype A. The results have implications for the classical concept of C. albicans serotypes and for the serological diagnosis of candidosis in relation with the previously described strain P variable antigens.
Sabouraudia 07/1983; 21(2):99-112.
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ABSTRACT: Cytochemical localization of wheat germ agglutinin binding sites in the cell wall of Candida albicans was investigated with fluorescence and electron microscopy. Various analytical techniques were employed in order to obtain a good penetration of the cytochemical markers, glycosylated horseradish peroxidase or glycosylated ferritin. In blastospores sectioned by cryostatic methods, a weak and continuous labelling of the blastospore periphery was observed with peroxidase, whereas bud scars and inner cell wall areas were labelled with ferritin. Following enzymatic treatment with pronase whose efficiency was followed by the periodic acid-thiocarbohydrazide -- Silver proteinate technique, the inner cell wall layers of bud are strongly stained with both fluorescein and the reactions products of peroxidase. After pronase-chitinase treatment, fluorescence was observed only in the mother cell wall. Finally, ultrathin glycol methacrylate sections showed a labelling both in inner and outer layers. All these results suggest that chitin was essentially distributed in the inner wall layers near the plasmalemma and in a smaller amount in outer wall layers. On the basis of the present findings, a hypothesis of wall assembly is proposed.
European Journal of Cell Biology 01/1982; 26(1):121-8. · 2.81 Impact Factor
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Journal of Ultrastructure Research 05/1981; 75(1):50-9.
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ABSTRACT: Immunological comparison among Beauveria species and Metarhizium strains isolated from insect larvae were made using immunoelectrophoresis. Antigens consisted of somatic or metabolic buffer-soluble proteins. Antisera were produced in rabbits against each of the 6 fungal strains. Each antigen was tested against the homologous antiserum non-adsorbed or cross-adsorbed with one other antigen and against the heterologous antisera. The immunoelectrophoresis slides were analyzed in terms of the number of precipitin arcs obtained with cross-reacting and reference antigens, with any one antiserum. Immunoelectrophoregrams were also considered.These tests revealed numerous antigenic similarities between different isolates. Moreover, results indicate that distinct and reproducible differences exist, both between two species of Beauveria and between four strains of M. anisopliae. The groupings of strains obtained from immunoelectrophoretic data correspond well with those based on spore morphology. Some methodological aspects are discussed. The present results are in agreement with our preliminary studies. They confirm that immunoelectrophoresis proves to be a very useful aid for the characterization of the Hyphomycetes candidates for biological control of insect pests.
Beauveria bassiana et B. brongnianii d'une part et 4 souches de Metarhizium anisopliae d'autre part, ont t analyss par immunolectrophorse de leurs protines cellulaires ou mtaboliques. Chaque antigne a t prouv contre le srum homologue non adsorb ou adsorb avec un antigne htrologue et contre les srums htrologues. Les parents et les diffrences entre les 2 Beauveria et entre les 4 Metarhizium ont t dfinies quantitativement par le nombre d'arcs de prcipitation communs ou specifiques. Nous avons aussi considr les immunoelectrophorgrammes des divers isolats.Les rsultats des ractions croises montrent d'importantes communauts antigniques entre les pathotypes tudis, toutefois l'utilisation de l'immunoadsorption a permis de mettre en vidence les fractions spcifiques aussi bien entre les 2 Beauveria qu'entre les 4 souches de l'espce M. anisopliae. Les distinctions tablies sur les donnes srologiques correspondent aux diffrences morphologiques sporales entre isolats. Ces rsultats prcisent et confirment nos tudes prliminaires en dmontrant l'intrt de la caractrisation immunolectrophortique des Hyphomyctes candidats la lutte biologique contre les insectes nuisibles.
Mycopathologia 12/1980; 75(2):101-108. · 1.65 Impact Factor
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ABSTRACT: Triacylglycerides (TAG), total cholesterol (TC) and serum lipoproteins (SLP) were studied during the course of 12 cases of human malaria and one case of simian inui malaria. The erythrocytic schizogony induced quickly an increase of TAG, a decrease of TC and modifications of SLP such an increase of VLDL parallel with a decrease or a disappearance of HDL. These modifications, always clear-cut, were not correlated with the intensity of parasitaemia or the species of Plasmodium. They were transient but longer than those described in connection with experimental rodent malaria. The changes of HDL could be associated with defective triglyceride removal of the plasma compartment due to an inhibition of the lipoprotein lipase induced by the parasite.
Pathologie Biologie 10/1980; 28(7):457-60. · 1.53 Impact Factor
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ABSTRACT: Candida albicans was grown in the darkness, at 28 degrees C, in a synthetic medium in which glucose and nitrogen concentrations were varied. Numeric appraisal of the chlamydospore index was possible only in the medium where glucose concentration was 0,08 g/1 or less. When the glucose concentration raised, pseudomycelial thalli bore numerous chlamydospores but sometimes also chains of cells with a dense granular content. These thalli bud yeast cells which separate and bud again in the medium. The different morphological aspects of the cultures are decribed, illustrated and classified according to the glucose and nitrogen concentration of the medium.
Mycopathologia 08/1980; 71(2):113-8. · 1.65 Impact Factor
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ABSTRACT: The mechanisms governing the development of local immunity in experimental dermatophytosis were studied by injecting intravenously trichophytin in guinea pigs cured of a prior Trichophyton mentagrophytes infestation. Dermal cell modifications were observed which were greater in the healed zones than in those not affected during the prior dermatophyte inoculation. These modifications included lymphocyte activation and accumulation and an accumulation of basophilic leukocytes. These observations suggest that after an acute dermatophyte infection heals, immunocompetent cells remain which are more numerous at the sites of lesions and that these cells would be responsible for the increased rate of elimination of the fungus during a reinfection. This hypothesis is discussed in the framework of the relationships observed in dermatophyte infections between delayed type hypersensitivity and resistance.
Journal of Investigative Dermatology 05/1980; 74(4):205-9. · 6.31 Impact Factor
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Annals of Tropical Medicine and Parasitology 03/1980; 74(1):17-28. · 1.43 Impact Factor
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ABSTRACT: Candida albicans a t cultiv l'obsurit, 28 C, dans un milieu de culture synthtique liquide dans lequel on fait varier les concentrations de glucose et d'azote. Des indices de chlamydosporulation ont pu etre calculs pour les milieux contenant au plus 0,08 g/l de glucose. Lorsque la concentration de glucose est plus importante, les thalles pseudomycliens portent, dans certains cas, de nombreuses chlamydospores, mais aussi des chaines de cellules contenu dense; ces thalles bourgeonnent des lments levuriformes qui se dtachent et continuent bourgeonner dans le milieu de culture. Les diffrentes morphologies rencontres sont dcrites, illustres et ordonnes en fonction des concentrations de glucose et d'azote du milieu.
Candida albicans was grown in the darkness, at 28 C, in a synthetic medium in which glucose and nitrogen concentrations were varied. Numeric appraisal of the chlamydospore index was possible only in the medium where glucose concentration was 0,08 g/l or less. When the glucose concentration raised, pseudomycelial thalli bore numerous chlamydospores but sometimes also chains of cells with a dense granular content. These thalli bud yeast cells which separate and bud again in the medium. The different morphological aspects of the cultures are decribed, illustrated and classified according to the glucose and nitrogen concentration of the medium.
Mycopathologia 12/1979; 71(2):113-118. · 1.65 Impact Factor
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ABSTRACT: La localisation ultrastructurale des polysaccharides parittaux de Candida albicans a t ralise par utilisation de Concanavaline A sur sections ultrafines de rsine hydrosoluble (glycol mthacrylate). Les sites rcepteurs de la lectine sont rvls en incubant successivement les coupes avec de la proxydase, puis un mlange 3-3 diaminobenzidine-H2O2. Cette mthode a permis de localiser les mannanes, qui sont les seuls polysaccharides paritaux de C. albicans susceptibles de ragir avec la Concanavaline A. Deux couches continues ractives ont t ainsi mises en vidence la priphrie de la paroi des blastospores. Les rsultats sont discuts en fonction de ceux obtenus par une mthode diffrente de mise en vidence des polysaccharides (PATAg).The ultrastructural localization of polysaccharides in the cell wall of Candida albicans was carried out by means of Concanavalin A on glycol methacrylate ultrathin sections. The sections were incubated successively with horseradish peroxydase, 3-3 diaminobenzidine and H2O2, for revealing the binding sites of the lectin. This method allowed us to localize mannan, since Concanavalin A does not react with other polysaccharides of the C. albicans cell wall. In these conditions mannan was found to be located in two continuous reactive layers at the periphery of blastospores cell wall. The results are discussed in relation with those obtained by another method using the polysaccharide detection technique described by Thiery (PATAg).
Archives of Microbiology 11/1979; 123(3):245-249. · 1.43 Impact Factor
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Journal of Parasitology 11/1979; 65(5):685-91. · 1.40 Impact Factor
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ABSTRACT: The ultrastructural localization of polysaccharides in the cell wall of Candida albicans was carried out by means of Concanavalin A on glycol methacrylate ultrathin sections. The sections were incubated successively with horseradish peroxydase, 3-3' diaminobenzidine and H2O2, for revealing the binding sites of the lectin. This method allowed us to localize mannan, since Concanavalin A does not react with other polysaccharides of the C. albicans cell wall. In these conditions mannan was found to be located in two continuous reactive layers at the periphery of blastospores cell wall. The results are discussed in relation with those obtained by another method using the polysaccharide detection technique described by Thiery (PATAg).
Archives of Microbiology 02/1979; 123(3):245-9. · 1.43 Impact Factor
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ABSTRACT: An extract from living yeast forms of S. schenckii was prepared. The yeasts originated from a shake culture in B.H.I. broth (Difco) incubated for 3 days at 35 degrees C in darkness; they were harvested, washed and disrupted with glass beads in a model MSK Braun mechanical cell homogenizer; a freezing-thawing was added to improve the extract. After electrophoretic separation in agarose gel, the extract's components were characterized by their enzymic activity; with this technique, 30 bands were revealed. These enzymic activities were also investigated on the antigenic fractions of the extract revealed by a rabbit hyperimmunserum: 16 among 22 immunoprecipitates are identified by their catalytic properties. Study of the earliest precipitating antibodies (appearing-order and enzymic caracterization) in rabbits just immunized completes this work. How to ameliorate the quality of the extract by culture and extraction conditions is also specified.
Mycopathologia 08/1978; 63(2):105-11. · 1.65 Impact Factor
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Mycopathologia 08/1978; 63(2):81-8. · 1.65 Impact Factor
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Mykosen. Supplement 02/1978; 1:218-21.
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ABSTRACT: 14 enzymatic activities (3 dehydrogenases and 11 hydrolases) were systematically investigated in the extracts of 2 strains of Aspergillus fischeri and 14 strains of A. fumigatus (8 isolated from human or animal lesions named 'pathogenic' strains and 6 strains of saprophytic origin). The enzymic composition of A. fumigatus appeared to be very different from one strain to another but no relationships could be established between enzymic differences and origin of the strains. In addition, the immunoelectrophoretic analysis of these antigenic extracts using rabbits immunsera and sera of patients with pulmonary asperigllosis was performed. The type II chymotrypsic activity, corresponding to C arc, was shown more frequently and gave stronger reaction when antigenic extracts from pathogenic strains were allowed to react with sera of patients. Moreover, when sera of patients revealed a same enzymic activity in a saprophytic strain and a pathogenic strain, the number of precipitin lines was significantly larger with the latter one. Accordingly, antigenic extracts used for precipitin tests had to be preferably prepared from pathogenic strains and tested, for value, against sera of patients with pulmonary aspergillosis.
Mycopathologia 10/1976; 59(2):81-90. · 1.65 Impact Factor
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ABSTRACT: The ultrastructure of T. mentagrophytes hyphal walls was studied after the use of a cytochemical reaction for polysaccharides. The sections showed a wall composed of 3 layers viz: an external, poorly reactive one with a microfibrillary structure; a medium, fairly reactive one, with a periodic structure and an internal, intensivly stained layer, not observed in old cells. Other observations suggested that the septal medium layer should be differentiated from those cited above. The results are compared with previous data and a synthetic scheme of the Trichophyton mentagrophytes hyphal wall is proposed.
Sabouraudia 12/1975; 13(3):244-54.
