Guo-ying Zhu

Publications (11)14.73 Total impact

• Article: [Inhibitory effects of breast cancer cells on proliferation and differentiation of osteoblasts].

  Guo-Ying Zhu, Yan-Yan Zhang, Shu-Zhu Gu, Xiao Chen
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  ABSTRACT: The effect of breast cancer cells on the normal function of osteoblasts remains unclear. This study was to investigate the effect of conditioned medium from two types of human breast cancer lines on osteoblastic proliferation, differentiation and mineralization. Fetal rat carvarial cells were treated with conditioned medium from MCF-7 estrogen-receptor(ER) positive, or MDA-MB-231 ER negative, breast cancer cells. Proliferation was measured by MTT assay, alkaline phosphatase (ALP) activity was assessed by the p-nitrophenyl phosphate (PNPP) method, and mineralized nodules were confirmed by alizarin red S (ARS) staining and the area of bone nodules was measured to observe the mineralization capacity. When osteoblasts were treated with conditioned medium from MDA-MB-231 or MCF-7, the cells became long and spindle-like, assumed a fibroblastic morphology. Conditioned medium from breast cancer cells inhibited proliferation and differentiation of osteoblasts, compared with those cultured with vehicle control medium. The proliferation inhibition rates of culture with 50% conditioned medium from MDA-MB-231 and MCF-7 cells on osteoblasts were 18.1%, 13.0%, 19.2%, 19.3% and 15.8%, 20.8%, 33.9%, 28.7% on day 1, day 3, day 5, and day 7, respectively (P<0.01). Moreover, incubation with conditioned medium inhibited ALP activity and bone-nodule formation of osteoblasts in a dose-dependent manner. Addition of 50% conditioned medium from MDA-MB-231 and MCF-7 cells inhibited ALP activity by 31.9% and 47.5% (P<0.01), and diminished the area of bone nodules by 89% and 74%, respectively, compared with the vehicle control group. Breast cancer cells inhibit proliferation, decrease ALP activity and disrupt the mineralization process of osteoblasts.
  Ai zheng = Aizheng = Chinese journal of cancer 06/2009; 28(5):449-55.
• Article: The efficacy and safety of calcitriol and/or Caltrate D in elderly Chinese women with low bone mass.

  Wei-bo Xia, Zhong-lan Zhang, Hong-fu Wang, Xun-wu Meng, Ying Zhang, Guo-ying Zhu, Xiao-ping Xing, Jian-li Liu, Li-hua Wang, Yan Jiang, Shi-fang Weng, Tao Xu, Ying-ying Hu, Wei Yu, Jun-ping Tian
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  ABSTRACT: To observe the efficacy and safety of Rocaltrol (calcitriol) and/or Caltrate D (calicum carbonate plus vitamin D) in elderly Chinese women with osteopenia or osteoporosis. One hundred fifty Chinese women aged over 65 years with osteopenia or osteoporosis from three centers were randomly divided into two groups. Seventy-six participants received Caltrate D as one pill daily; the other 74 participants received 0.25 mug Caltrate D plus Rocaltrol daily. The changes in bone mineral density (BMD) served as primary end-points. Height changes, the presence of new vertebral fractures, muscle strength and balance were evaluated. The following are the mean percentage changes (and SD) in BMD over 12 months: at L2-L4, 0.83+/-3.88 in the Caltrate D group and 2.84+/-4.04 in the Rocaltrol+Caltrate D group (P=0.003, by ANCOVA); at the femoral neck, 0.04+/-3.94 in the Caltrate D group and 2.01+/-5.45 in the Rocaltrol+Caltrate D group (P=0.085, by ANCOVA); and in the trochanter, 1.59+/-4.57 in the Caltrate D group and 3.76+/-6.25 in the Rocaltrol+Caltrate D group (P=0.053, by ANCOVA). The stand and maximal forward reach test (SMFRT) was significantly enhanced in both groups during the 12 months of treatment, but no significant differences were found between these two groups. No severe adverse event related to these medications occurred throughout the study. Treatment with Rocaltrol plus Caltrate D or Caltrate D for 12 months in elderly Chinese postmenopausal women effectively increased BMD at the lumbar spine. Rocaltrol plus Caltrate D was more effective at the lumbar spine than Caltrate D alone.
  Acta Pharmacologica Sinica 04/2009; 30(3):372-8. · 1.95 Impact Factor
• Article: The efficacy and safety of calcitriol and/or Caltrate D in elderly Chinese women with low bone mass

  Wei-bo Xia, Zhong-lan Zhang, Hong-fu Wang, Xun-wu Meng, Ying Zhang, Guo-ying Zhu, Xiao-ping Xing, Jian-li Liu, Li-hua Wang, Yan Jiang, Shi-fang Weng, Tao Xu, Ying-ying Hu, Wei Yu, Jun-ping Tian
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  ABSTRACT: Aim: To observe the efficacy and safety of Rocaltrol (calcitriol) and/or Caltrate D (calicum carbonate plus vitamin D) in elderly Chinese women with osteopenia or osteoporosis.
  Acta Pharmacologica Sinica 02/2009; 30(3):372-378. · 1.95 Impact Factor
• Article: Safety and efficacy of the CYPHER Select Sirolimus-eluting stent in the "Real World"--clinical and angiographic results from the China CYPHER Select registry.

  Run-Lin Gao, Bo Xu, Shu-Zheng Lu, Ji-Lin Chen, Ya-Ling Han, Jun-Zhu Chen, Lu-Yue Gai, Jun-Bo Ge, Wei-Min Wang, Zhi-Min Du, [......], Ji-Yan Chen, Ben He, Guo-Liang Jia, Zhi-Jian Yang, Ke-Jiang Cao, Wei-Min Li, Wei-Feng Shen, Zheng Wan, De-Jia Huang, Guo-Ying Zhu
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  ABSTRACT: This post-marketing surveillance registry is aimed at determining the safety and reliability of the CYPHER Select Sirolimus-eluting stent (SES) in routine clinical practice. Little information and angiographic follow-up data in large-scale "real world" registry is available for the CYPHER Select SES, an advanced-generation SES. This was a prospective multicenter (20 centers) registry. 1189 consecutive patients who received at least 1 CYPHER Select SES during daily clinical practice were enrolled. Patients who underwent emergency stenting for acute myocardial infarction were excluded. The procedure's success rate was 98.3% for CYPHER Select SES implantation, and follow-up rates were 98% with 100% data auditing. Target lesion revascularization (TLR) at 12 months occurred in 60 (5.14%) cases, cardiac death in 13 cases (1.11%), Q wave myocardial infarction (MI) in 5 cases (0.43%), non-Q-MI in 9 cases (0.77%), target vessel revascularization (TVR) in 67 cases (5.74%), and MACE defined as cardiac death, nonfatal MI and TLR in 76 cases (6.51%). MACE-free survival rate at 12 months was 93.7%. Angiographic follow-up at 9 months was performed in 418 (68.3%) lesions treated by CYPHER Select SES. The binary restenosis rate was 4.8% in-stent and 9.6% in-segment. Subgroup analysis showed diabetes, bifurcation lesion and combined use of different stents were independent risk factors of cumulative MACE. In-segment MLD </=2.25 mm at post-procedure and ostial lesion was independent predictors of in-segment restenosis. In this registry, the MACE, TLR, angiographic late loss and binary restenosis rates of CYPHER Select SES were similar to those reported in SES randomized trials and "real world" registries. The safety and efficacy of CYPHER Select SES shown in this registry are consistent with those seen in SES studies.
  International journal of cardiology 04/2008; 125(3):339-46. · 7.08 Impact Factor
• Article: [Effects of occupational lead exposure on lumbar vertebral fracture in male workers.].

  Yi Sun, Dong-Hong Sun, Guo-Ying Zhu, Li-Jian Lei, Tai-Yi Jin
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  ABSTRACT: To investigate the effects of occupational lead exposure on lumbar vertebral fracture in exposed male workers. One hundred and fifty-two lead-exposed male workers in a storage battery plant in Shanghai were selected as the study population. The blood lead (BPb) and the urinary lead (UPb) were measured by graphite-furnace atomic absorption spectrometry (GF-AAS). Bone mineral density (BMD) was measured by the monophoton absorptiometry(SPA-4) and Z score was determined. Anteroposterior and lateral lumbar spinal X-ray films were taken to determine lumbar vertebral fracture. For the occupationally lead-exposed workers, geometric mean of BPb was 0.85 (0.33 approximately 1.90) micromol/L, geometric mean of UPb was 4.84 (0.46 approximately 21.31) microg/g Cr, and the prevalence of lumbar vertebral fracture was 19.7%. The prevalence of lumbar vertebral fracture would increase with the increase of age and work year, but with no significantly statistical difference (P > 0.05). The bone mineral density (BMD) would decrease with the increase of BPb and UPb (P < 0.05). The prevalence of lumbar vertebral fracture would increase significantly with the increase of the lead exposure (P < 0.05) with the linear correlation (P < 0.05). The prevalence of lumbar vertebral fracture would increase significantly with the decrease of the bone mass (P < 0.01) with the linear correlation (P < 0.01). The occupational exposure to lead could cause the decrease of the bone mineral density and the increase of the prevalence of lumbar vertebral fracture. The development of lumbar vertebral fracture is associated with the decrease of bone mass.
  Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases 12/2007; 25(11):645-8.
• Article: [Effects of occupational lead exposure on bone mineral density and bone metabolism in workers].

  Yi Sun, Tai-yi Jin, Dong-hong Sun, Guo-ying Zhu, Li-jian Lei, Hai-ying Zhang, Guang-zu Shen
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  ABSTRACT: To investigate the effects of occupational lead exposure on the bone mineral density and the bone metabolism in exposed workers. Two hundred and ninety-eight lead-exposed workers in a storage battery plant in Shanghai were selected as the exposed subjects while eighty-one healthy officers in the plant who were not occupationally exposed to lead were treated as the control. The blood lead (BPb) and the urinary lead (UPb) were used as the exposure biomarkers while the Z score, the urinary hydroxyproline (HYP) the serum alkaline phosphatase (ALP) the serum alkaline phosphatase bone isoenzyme BALP and the serum osteocalcin BGP were used as the effect biomarkers for the bone effect caused by the lead. Bone mineral density (BMD) was measured by the single-photon absorptiometry (SPA-4). The BPb, UPb, HYP, ALP, BALP in the occupational lead exposure group were higher than those in the control group with significantly statistical difference in male (P < 0.01). The levels of BGP in the exposure group was higher than that in the control group without significantly statistical difference (P > 0.05). The BMD in the exposure group was lower than that in the control group without significantly statistical difference (P > 0.05). The BMD was significantly decreased in the groups of the UPb 10 approximately microg/g Cr level compared with the 0 approximately microg/g Cr group with the significant difference (P < 0.01). In males, the BMD was significantly decreased in the group of the BPb 300 approximately microg/L level compared with the 0 approximately microg/L group with the significant difference (P < 0.01). The levels of HYP, ALP, BALP, BGP in the UPb 20 approximately microg/g Cr group were significantly higher than those in the UPb 0 approximately microg/g Cr group (P < 0.05). The levels of HYP, ALP, BALP, BGP in the BPb 300 approximately microg/L group were significantly higher than those in the BPb 0 approximately microg/L group (P < 0.05). The prevalence of both osteoporosis and the abnormal bone metabolisms indexes would increase significantly with the increase of the lead exposure (P < 0.01) with the linear correlation (P < 0.01). But the prevalence of higher BGP had no significant correlation with UPb (P > 0.05). BMDs were calculated using BMDS Version 1.3.2 software and BMDLs were also determined. The BMDLs of BPb and UPb for lead-induced osteoporosis were higher than those representing the change of bone metabolism induced by lead. The occupational exposure to lead could cause the decrease of the bone mineral density, lead to the osteoporosis, and may affect the bone metabolism.
  Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases 06/2007; 25(5):257-62.
• Article: The Chinese herbal preparation Qing Yi Tang (QYT) improves intestinal myoelectrical activity and increases intestinal transit during acute pancreatitis in rodents.

  Yong-Yu Li, Andrei Sibaev, Ming-Zheng Zhou, Guo-Ying Zhu, Birol Yüce, Martin Storr
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  ABSTRACT: The aim was to investigate alterations of intestinal motility in models of acute pancreatitis and to investigate the effects of the Chinese herbal preparation Qing Yi Tang (QYT) on these alterations. Upper gastrointestinal transit was evaluated in mice following induction of mild acute pancreatitis (MAP) using caerulein. Myoelectrical activity was recorded in rats after induction of severe acute pancreatitis (SAP) using sodium deoxycholate (SDOC). The contractility of jejunum segments was evaluated in the presence of SDOC, lipopolysaccharide (LPS) and trypsin. QYT accelerated the transit in MAP mice in a concentration dependent manner. Slow wave activity of smooth muscle in rat stomach and jejunum remained unchanged following SAP, but the spiking activity was significantly decreased, with bursts of 7.2 +/- 2.6/10 min compared with 47.9 +/- 13.2/10 min without SAP (p < 0.01). QYT reversed this decrease. Additionally, the amplitudes of slow waves and spikes were enhanced by QYT in SAP rats. The tension and amplitude of spontaneous contractile activity was reduced by SDOC and LPS and increased by trypsin. Gastrointestinal (GI) transit is altered by SAP but not by MAP. The Chinese herbal preparation QYT improves disturbed motility in AP by stimulating myoelectrical activity and accelerating GI transit.
  Phytotherapy Research 05/2007; 21(4):324-31. · 2.09 Impact Factor
• Article: [Development of a simple predicting tool for low bone mass of postmenopausal women: a study in Shanghai].

  Qi-ren Huang, Zhen-lin Zhang, Qi Zhou, Guo-ying Zhu, Yue-juan Qin, Hao Zhang, Yun-qiu Hu, Miao Li, Yu-juan Liu
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  ABSTRACT: To develop a simple screening tool for low bone mass of postmenopausal women. 405 postmenopausal women in Shanghai who visited the department of osteoporosis consecutively, aged 62.8 +/- 8.0 (47 approximately 90), underwent questionnaire survey on the risk factors of osteoporosis and fracture. Dual energy X-ray absorptiometry (DXA) was conducted on the left or right femoral neck to measure the bone mineral density (BMD) to identify osteoporosis (T-score <or= -2.5). Univariate linear regression was conducted to identify the variables with significant association with the femoral neck BMD to be used in multiple variable regression analysis. The screening index was obtained by the formula: index = independent variable X corresponding weight. A receiver operating characteristic (ROC) curve was drawn with the sensitivity (true positive rate) as the vertical coordinate and 1-specificity (false positive rate) as the horizontal coordinate. The area under the ROC curve (AUC ROC) was calculated so as to establish a parsimonious model. The final tool was based only on age and body weight. The formula of screening index was: index = 2 x weight (kg)/10 + [-1 x age (year)/10]. The risk index thus obtained had a sensitivity of 93.4%, a specificity of 52.6%, and an AUC ROC of 0.818 (95% CI for the mean: 0.766 approximately 0.870). 58% of the high-risk women had osteoporosis, compared with 26% and 2% of the intermediate and low-risk women respectively. The established and verified screening tool can easily predict the bone mass status in the postmenopausal women, thus screening the high-risk population and saving the cost of BMD measurement unnecessary for the low-risk population.
  Zhonghua yi xue za zhi 03/2007; 87(12):808-11.
• Article: Alteration of the pancreatic endocrine component in the early stage of acute necrotic pancreatitis in rats.

  Yong Yu Li, Guo Ying Zhu, Yong Xin Gao, Zhi Yong Yang, Zhu Yi Wang
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  ABSTRACT: To investigate alterations of the pancreatic endocrine component in the early stage of acute necrotic pancreatitis (ANP) in rats. Thirty-six Sprague-Dawley rats were randomly allocated to two groups: ANP group (n = 18) and sham-operated (control) group (n = 18). ANP was induced by retrograde injection of 4% sodium deoxycholate (40 mg/kg, 0.1 mL/min) into the biliopancreatic duct and the severity of pancreatitis induced was assessed by histopathological examination and level of plasma amylase. The pancreatic endocrine function was assessed by measuring the levels of plasma glucose and insulin and by measuring the insulin content in pancreatic beta cells by immunofluorescence and immunocytochemistry. Five hours after operation, the pancreas of rats in the ANP group showed pathological changes with edema, hemorrhage, fatty necrosis, acinar destruction and leukocyte infiltration in the exocrine portion of the pancreas. Plasma amylase activity increased significantly (P < 0.01) and bloody ascites appeared in the abdominal cavity. Nevertheless the endocrine islets appeared normal and the beta cells contained intensive labeling of insulin. Levels of glucose and insulin in plasma increased significantly. In the ANP group, 5 h after operation the plasma level of glucose was 8.18 +/- 2.26 mmol/L vs 6.39 +/- 1.26 mmol/L, and of insulin was 23.27 +/- 3.50 MIU/L vs 18.40 +/- 3.98 MIU/L. In the control group, 5 h after operation the plasma level of glucose was 9.39 +/- 0.62 mmol/L vs 5.89 +/- 0.62 mmol/L, and of insulin was 26.28 +/- 4.77 MIU/L vs 12.89 +/- 2.05 MIU/L; there was no significant difference between these two groups (P > 0.05). After a bolus injection of glucose, however, a much higher level of insulin was found in the control group (35.30 +/- 5.05 MIU/L) than that in the ANP group (23.91 +/- 4.62 MIU/L, P < 0.05). There may be an impaired ability of insulin release in response to glucose stimulation in the early stage of ANP, although the morphology of the pancreatic endocrine component remains intact.
  Chinese Journal of Digestive Diseases 02/2006; 7(3):164-9.
• Article: [Application of benchmark dose (BMD) in a bone-effect study on a general population environmentally exposed to cadmium].

  Hai-lei Qian, Tai-yi Jin, Qing-hu Kong, Hong-fu Wang, Guo-ying Zhu
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  ABSTRACT: To estimate the benchmark dose for osteoporosis caused by cadmium exposure in a Chinese general population with an epidemiological study. The inhabitants living in both cadmium polluted and non-polluted areas served as the exposure group and the control group. Urinary cadmium (UCd) and Blood cadmium (BCd) were used as exposure biomarkers while the Z score was used as effect biomarker for the osteoporosis. The UCd and BCd in the habitants of the polluted areas were significantly higher than those in the habitants of the control area on average (P < 0.05) and the UCd and BCd in the habitants of the highly polluted areas were significantly higher than those in the habitants of the moderately polluted area on average (P < 0.05). The bone mineral density was significantly decreased in the groups of the highest UCd and BCd level compared with the 5 microg/g Cr group with the significant difference (P < 0.05). The morbidity of the osteoporosis would increase significantly with the increase of the cadmium exposure (P < 0.05) with the linear correlation (P < 0.05). BMDs were calculated using BMDS Version l.3.2 software and BMDLs were also determined. The BMDL of UCd for cadmium-induced osteoporosis was higher than those representing cadmium-induced renal dysfunction. High level of cadmium exposure can induce osteoporosis, which occurs later than renal damage related to cadmium exposure. The BMD is a practical method.
  Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases 01/2006; 24(1):23-6.
• Article: Investigation of factors on a fungal biofilter to treat waste gas with ethyl mercaptan.

  Guo-Ying Zhu, Jun-Xin Liu
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  ABSTRACT: The biofilter is cost-effective for the waste gases treatment. The bacterial is the main microorganism in the conventional biofilters. However, it faces some problems on the elimination of hydrophobic compounds. In order to overcome these problems, the biofilters with fungi were developed. The objective of this study is to investigate the factors affecting ethyl mercaptan (EM)-degradation using a fungal biofilter. A laboratory experiment was set up. The effects of loading rate, empty bed residence times (EBRT) and pH on EM degradation were investigated. Over 95% removals of EM could be achieved, under the condition of the influent loadings below 50 g/(m x h). Removal efficiencies improved to 98% with EM loading decreased to 45 g/(m x h). For long EBRT of 58 s corresponding to a low rate of 0.3 m3/h, the EM removal efficiencies of over 98% were observed. However, when EBRT was decreased to 14 s, the removal efficiencies fell under 80%. The pH range of 3-5 was feasible to fungi.
  Journal of Environmental Sciences 02/2004; 16(6):898-900. · 1.66 Impact Factor