[Show abstract][Hide abstract] ABSTRACT: Objective. Many investigators have studied the expression of G1 phase regulatory protein in uterine cervical cancer. However, it is unclear which step of the genetic expression participates in cyclin D1 expression and what its prognostic meaning is. The aims of this study were to evaluate the regulatory level of cyclin D1 expression and the relationship between the expression of cyclin D1 and its inhibitor, p21WAF1/CIP1, and to evaluate their impact on the prognosis of early stage cervical cancer.
[Show abstract][Hide abstract] ABSTRACT: Purpose: We carried out this study to evaluate the biological significance of phospholipase C 1 gene mutation in mouse sperm in the acrosome reaction, fertilization, and embryo development.Methods: Study subjects were divided into two groups according to the sperm [intact phospholipase C (PLC) 1 and PLC 1–/– C57BL/6J CBA F1 mouse sperm] used. The positive acrosome reaction rate labeled with fluorescein isothiocyanate–Pisum sativum agglutinin, the fertilization rate, and the rate of embryos developed to the stage of morula or blastocyst in the two groups were compared.Results: The mouse sperm null for the PLC 1 gene showed a lower acrosome reaction rate than control sperm (69.2 vs 50.9%, P < 0.05).="" and="" the="" fertilization="" rate="" and="" the="" rate="" of="" embryos="" developed="" to="" the="" stage="" of="" morula="" or="" blastocyst="" were="" also="" lower="" in="" the="" group="" using="" plc="">1–/– mouse sperm compared to the intact group (P < 0.05;="" 73.5="" vs="" 51.8%="" and="" 15.7="" vs="" 4.3%,="">Conclusions: Mutation of the PLC 1 gene in the mouse sperm reduces the acrosome reaction rate, fertilization rate, and embryo development rate, which may be the etiologic factors responsible for the low reproductive rate of PLC 1–/– mouse.
Journal of Assisted Reproduction and Genetics 04/2001; 18(5):305-310. DOI:10.1023/A:1016622519228 · 1.72 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Purpose: This study was carried out to investigate the efficacyof electric stimulation before and/or after intracytoplasmicsperm injection (ICSI) on bovine oocyte activation andembryo development.
Methods: The oocytes were treated with electric shock before(B), before and after (B&A), and after (A) sperm injection.In each group, sham ICSI (ICSI-s) was performed to excludethe effect of parthenogenesis (B ICSI-s, B&A ICSI-s, and AICSI-s). An electric pulse was applied with a single directcurrent (DC) pulse (0.8 kV/cm, 70 sec).
Results: One pronucleus (PN) formation in the B&A ICSI-sgroup was slightly higher than that found in B and B&AICSI group; however, the difference was not significant. TwoPN formation in B&A ICSI group was higher than that foundin sham ICSI groups (P < 0.05).="" there="" were="" no="" differencesamong="" treatment="" groups="" in="" the="" cleavage="" rate;="" however,="" morulaeand="" blastocyst="" formation="" in="" the="" b&a="" embryos="" wassignificantly="" higher="" than="" that="" of="" other="" groups="">P < 0.05)and="" got="">
Conclusions: Electric stimulation before and after injectionwas an effective method in inducing bovine oocyte activationand in sustaining embryo development to the morulae andblastocyst stage.
Journal of Assisted Reproduction and Genetics 06/2000; 17(6):310-314. DOI:10.1023/A:1009496726343 · 1.72 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Objectives. It has been shown that heat shock proteins (HSPs) protect cells from death caused by various noxious stimuli. Overexpression of HSP70 seems to be related to hormonal regulation of cell proliferation and/or down-regulation of sex steroid receptors. Wild-type p53 has been reported to repress HSP70 gene expression. It has been shown that mutant p53–HSP70 complex is highly expressed in cancer. However, the relationship between HSPs and steroid receptors or tumor suppressor gene products has not been well understood in uterine cervical carcinoma. This study was undertaken to examine the expression of HSP70, estrogen receptor (ER), and p53 in carcinoma of the uterine cervix. In addition, we analyzed HPV infection status and compared it to such immunohistochemical parameters. We also analyzed the relationship between these biological products and their clinicopathologic characteristics.Methods. Paraffin-embedded tissue sections were obtained from 84 patients with carcinoma of the uterine cervix. Expression of HSP70, p53, and ER was evaluated by immunohistochemical staining using anti-HSP70 monoclonal antibody (SPA810), anti-p53 (BP53.12), and ER1D5 antibody, respectively. PCR HPV detection was done by dot hybridization method.Results. Positive staining of HSP70 was detected in 73% of the cases. HSP70 positivity was significantly higher in stage I cervical cancer than in stages II–IV (P = 0.02). This was associated with neither tumor size, lymph node status, parametrial involvement status, nor tumor markers (TA-4). Furthermore, there was no significant correlation between HSP70 positivity and the expression of p53 or ER or HPV infection status.Conclusion. These data suggested that HSP70 positivity was frequent in uterine cervical cancer, especially in the early stages. However, this was not significantly correlated with clinicopathologic characteristics nor with the expression of p53 or ER nor with HPV infection in carcinoma of the uterine cervix.