Je-Ho Lee

Sungkyunkwan University, Seoul, Seoul, South Korea

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Publications (88)286.72 Total impact

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    ABSTRACT: Purpose: We carried out this study to evaluate the biological significance of phospholipase C 1 gene mutation in mouse sperm in the acrosome reaction, fertilization, and embryo development.Methods: Study subjects were divided into two groups according to the sperm [intact phospholipase C (PLC) 1 and PLC 1–/– C57BL/6J CBA F1 mouse sperm] used. The positive acrosome reaction rate labeled with fluorescein isothiocyanate–Pisum sativum agglutinin, the fertilization rate, and the rate of embryos developed to the stage of morula or blastocyst in the two groups were compared.Results: The mouse sperm null for the PLC 1 gene showed a lower acrosome reaction rate than control sperm (69.2 vs 50.9%, P < 0.05).="" and="" the="" fertilization="" rate="" and="" the="" rate="" of="" embryos="" developed="" to="" the="" stage="" of="" morula="" or="" blastocyst="" were="" also="" lower="" in="" the="" group="" using="" plc="">1–/– mouse sperm compared to the intact group (P < 0.05;="" 73.5="" vs="" 51.8%="" and="" 15.7="" vs="" 4.3%,="">Conclusions: Mutation of the PLC 1 gene in the mouse sperm reduces the acrosome reaction rate, fertilization rate, and embryo development rate, which may be the etiologic factors responsible for the low reproductive rate of PLC 1–/– mouse.
    Journal of Assisted Reproduction and Genetics 04/2001; 18(5):305-310. · 1.82 Impact Factor
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    ABSTRACT: Purpose: This study was carried out to investigate the efficacyof electric stimulation before and/or after intracytoplasmicsperm injection (ICSI) on bovine oocyte activation andembryo development. Methods: The oocytes were treated with electric shock before(B), before and after (B&A), and after (A) sperm injection.In each group, sham ICSI (ICSI-s) was performed to excludethe effect of parthenogenesis (B ICSI-s, B&A ICSI-s, and AICSI-s). An electric pulse was applied with a single directcurrent (DC) pulse (0.8 kV/cm, 70 sec). Results: One pronucleus (PN) formation in the B&A ICSI-sgroup was slightly higher than that found in B and B&AICSI group; however, the difference was not significant. TwoPN formation in B&A ICSI group was higher than that foundin sham ICSI groups (P < 0.05).="" there="" were="" no="" differencesamong="" treatment="" groups="" in="" the="" cleavage="" rate;="" however,="" morulaeand="" blastocyst="" formation="" in="" the="" b&a="" embryos="" wassignificantly="" higher="" than="" that="" of="" other="" groups="">P < 0.05)and="" got=""> Conclusions: Electric stimulation before and after injectionwas an effective method in inducing bovine oocyte activationand in sustaining embryo development to the morulae andblastocyst stage.
    Journal of Assisted Reproduction and Genetics 06/2000; 17(6):310-314. · 1.82 Impact Factor
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    ABSTRACT: Objectives. It has been shown that heat shock proteins (HSPs) protect cells from death caused by various noxious stimuli. Overexpression of HSP70 seems to be related to hormonal regulation of cell proliferation and/or down-regulation of sex steroid receptors. Wild-type p53 has been reported to repress HSP70 gene expression. It has been shown that mutant p53–HSP70 complex is highly expressed in cancer. However, the relationship between HSPs and steroid receptors or tumor suppressor gene products has not been well understood in uterine cervical carcinoma. This study was undertaken to examine the expression of HSP70, estrogen receptor (ER), and p53 in carcinoma of the uterine cervix. In addition, we analyzed HPV infection status and compared it to such immunohistochemical parameters. We also analyzed the relationship between these biological products and their clinicopathologic characteristics.Methods. Paraffin-embedded tissue sections were obtained from 84 patients with carcinoma of the uterine cervix. Expression of HSP70, p53, and ER was evaluated by immunohistochemical staining using anti-HSP70 monoclonal antibody (SPA810), anti-p53 (BP53.12), and ER1D5 antibody, respectively. PCR HPV detection was done by dot hybridization method.Results. Positive staining of HSP70 was detected in 73% of the cases. HSP70 positivity was significantly higher in stage I cervical cancer than in stages II–IV (P = 0.02). This was associated with neither tumor size, lymph node status, parametrial involvement status, nor tumor markers (TA-4). Furthermore, there was no significant correlation between HSP70 positivity and the expression of p53 or ER or HPV infection status.Conclusion. These data suggested that HSP70 positivity was frequent in uterine cervical cancer, especially in the early stages. However, this was not significantly correlated with clinicopathologic characteristics nor with the expression of p53 or ER nor with HPV infection in carcinoma of the uterine cervix.
    Gynecologic Oncology 08/1999; · 3.93 Impact Factor
  • Menopause-the Journal of The North American Menopause Society - MENOPAUSE. 01/1998; 5(4).
  • Menopause-the Journal of The North American Menopause Society - MENOPAUSE. 01/1997; 4(4).
  • Menopause-the Journal of The North American Menopause Society - MENOPAUSE. 01/1997; 4(4).
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    ABSTRACT: We have investigated a series of 19 human ovarian carcinomas and 17 borderline ovarian tumors to determine the loss of heterozygosity on chromosome 17p and possible concurrent p53 mutations. Allelic losses were assessed using restriction fragment length polymorphism study, and p53 gene mutations were detected by single strand conformation polymorphism analysis and by direct sequencing. In addition, we stained the same tumor sections immunohistochemically to detect p53 protein in tissues. Among 19 ovarian malignant tumor samples tested, we identified 17p allelic deletions in 12 (63.2%) of 19 informative cases. The p53 gene mutation was observed in 7 of 19 (36.8%) malignant ovarian tumors, and it was predominantly observed in tumors with allelic loss on 17p (six of seven tumors, 85.7%). Although 9 cases of 17 borderline ovarian tumors showed shifted bands on single strand conformation polymorphism analysis, only one case was proved to have a point mutation in direct sequencing. We also obtained six cases (31.6%) of positive immunoreactivity from 19 ovarian cancers and 3 cases (17.6%) from 17 borderline ovarian tumors. We conclude that loss or inactivation of tumor suppressor gene function by chromosome 17p allelic deletions or p53 mutations are important genetic changes in ovarian cancer.
    Cancer Genetics and Cytogenetics. 11/1995; 85(1):43-50.
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    ABSTRACT: To present a case of successful robotic assisted radical trachelectomy. A nulliparous woman with early cervical cancer underwent a laparo-scopic radical trachelectomy and pelvic lymphadenectomy with the da Vinci robot (Intuitive Surgical Inc., Sunnyvale, CA). After the pelvic lymph nodes were found negative on frozen section, the parametria, paracolpia and uterosacral ligaments were dissected transabdominally sparing the ascending branches of the uterine arteries. Cervical transection and vaginal closure were performed transvaginally. Surgical time was 450 min. No perioperative complications were noted. Robotic laparoscopic radical trachelectomy may bridge the gap between lapa-rotomy and laparoscopy for radical trachelectomy.

Publication Stats

1k Citations
286.72 Total Impact Points

Institutions

  • 2000–2012
    • Sungkyunkwan University
      • • Samsung Medical Center
      • • Molecular Therapy Research Center
      • • School of Medicine
      Seoul, Seoul, South Korea
  • 2002–2011
    • MEDIPOST Biomedical Research Institute
      Sŏul, Seoul, South Korea
  • 2010
    • Vaccine Research Institute of San Diego
      San Diego, California, United States
    • National Cancer Center Korea
      Kōyō, Gyeonggi Province, South Korea
    • Konyang University
      • College of Medicine
      Nonsan, South Chungcheong, South Korea
  • 2009
    • Yonsei University
      • Division of Biological Science and Technology
      Seoul, Seoul, South Korea
    • Konkuk University
      • Department of Obstetrics and Gynecology
      Sŏul, Seoul, South Korea
    • Chung-Ang University Hospital
      Sŏul, Seoul, South Korea
    • Ajou University
      • Department of Obstetrics and Gynecology
      Seoul, Seoul, South Korea
  • 2008–2009
    • Korea Basic Science Institute KBSI
      Sŏul, Seoul, South Korea
  • 2006–2007
    • Konkuk University Medical Center
      • Department of Obstetrics and Gynecology
      Changnyeong, South Gyeongsang, South Korea
  • 2001
    • University of Texas MD Anderson Cancer Center
      Houston, Texas, United States
    • Pohang University of Science and Technology
      • Department of Life Sciences
      Antō, North Gyeongsang, South Korea
  • 1999
    • Samsung Medical Center
      Sŏul, Seoul, South Korea