Je-Ho Lee

Sungkyunkwan University, Seoul, Seoul, South Korea

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Publications (88)296.86 Total impact

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    ABSTRACT: Purpose: We carried out this study to evaluate the biological significance of phospholipase C 1 gene mutation in mouse sperm in the acrosome reaction, fertilization, and embryo development.Methods: Study subjects were divided into two groups according to the sperm [intact phospholipase C (PLC) 1 and PLC 1–/– C57BL/6J CBA F1 mouse sperm] used. The positive acrosome reaction rate labeled with fluorescein isothiocyanate–Pisum sativum agglutinin, the fertilization rate, and the rate of embryos developed to the stage of morula or blastocyst in the two groups were compared.Results: The mouse sperm null for the PLC 1 gene showed a lower acrosome reaction rate than control sperm (69.2 vs 50.9%, P < 0.05).="" and="" the="" fertilization="" rate="" and="" the="" rate="" of="" embryos="" developed="" to="" the="" stage="" of="" morula="" or="" blastocyst="" were="" also="" lower="" in="" the="" group="" using="" plc="">1–/– mouse sperm compared to the intact group (P < 0.05;="" 73.5="" vs="" 51.8%="" and="" 15.7="" vs="" 4.3%,="">Conclusions: Mutation of the PLC 1 gene in the mouse sperm reduces the acrosome reaction rate, fertilization rate, and embryo development rate, which may be the etiologic factors responsible for the low reproductive rate of PLC 1–/– mouse.
    Journal of Assisted Reproduction and Genetics 04/2001; 18(5):305-310. DOI:10.1023/A:1016622519228 · 1.77 Impact Factor
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    ABSTRACT: Smad proteins activated by TGF-β form complexes with Smad4. Upon activation, these complexes translocate to the nucleus of the cell, where they induce transcription of genes related to inhibition of cell growth, cell differentiation and apoptosis. We investigated the role of Smads in the TGF-β–mediated signal-transduction cascade in 4 human cervical cancer cell lines: HeLa, Caski, HT-3 and SiHa. Based on our results, SiHa cells show low mRNA expression of mutated Smad4 (Gly230Ala, Ala488Val) and of Smads 2, 3, 5 and 6. SiHa cells were likewise defective in TGF-β signaling, as evidenced by a lack of significant growth inhibition following TGF-β treatment. In addition, TGF-β did not induce transcription of the PAI-1 gene or change Smad protein levels. Introduction of Smad3 and/or Smad4 into SiHa cells restored TGF-β signaling, as determined by activation of the 3TP-lux reporter gene and by prominent apoptotic cell death with PAI-1 induction. Analysis of the downstream targets activated by TGF-β yielded rapid activation of p38 with subsequent phosphorylation of the transcription factor ATF-2 but unchanged SAPK/JNK activation in the 4 cervical cancer cell lines. Our findings demonstrate that (i) decrease of Smad4 mRNA expression is closely associated with defective TGF-β response and lack of growth inhibition, (ii) activation of PAI-1 by TGF-β may be Smad4-dependent and (iii) the Smad and the p38 cascades are triggered by TGF-β independently of each other in human cervical cancer. © 2001 Wiley-Liss, Inc.
    International Journal of Cancer 01/2001; 94(4):500 - 507. DOI:10.1002/ijc.1494 · 5.01 Impact Factor
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    ABSTRACT: Purpose: This study was carried out to investigate the efficacyof electric stimulation before and/or after intracytoplasmicsperm injection (ICSI) on bovine oocyte activation andembryo development. Methods: The oocytes were treated with electric shock before(B), before and after (B&A), and after (A) sperm injection.In each group, sham ICSI (ICSI-s) was performed to excludethe effect of parthenogenesis (B ICSI-s, B&A ICSI-s, and AICSI-s). An electric pulse was applied with a single directcurrent (DC) pulse (0.8 kV/cm, 70 sec). Results: One pronucleus (PN) formation in the B&A ICSI-sgroup was slightly higher than that found in B and B&AICSI group; however, the difference was not significant. TwoPN formation in B&A ICSI group was higher than that foundin sham ICSI groups (P < 0.05).="" there="" were="" no="" differencesamong="" treatment="" groups="" in="" the="" cleavage="" rate;="" however,="" morulaeand="" blastocyst="" formation="" in="" the="" b&a="" embryos="" wassignificantly="" higher="" than="" that="" of="" other="" groups="">P < 0.05)and="" got=""> Conclusions: Electric stimulation before and after injectionwas an effective method in inducing bovine oocyte activationand in sustaining embryo development to the morulae andblastocyst stage.
    Journal of Assisted Reproduction and Genetics 06/2000; 17(6):310-314. DOI:10.1023/A:1009496726343 · 1.77 Impact Factor
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    ABSTRACT: Objectives. It has been shown that heat shock proteins (HSPs) protect cells from death caused by various noxious stimuli. Overexpression of HSP70 seems to be related to hormonal regulation of cell proliferation and/or down-regulation of sex steroid receptors. Wild-type p53 has been reported to repress HSP70 gene expression. It has been shown that mutant p53–HSP70 complex is highly expressed in cancer. However, the relationship between HSPs and steroid receptors or tumor suppressor gene products has not been well understood in uterine cervical carcinoma. This study was undertaken to examine the expression of HSP70, estrogen receptor (ER), and p53 in carcinoma of the uterine cervix. In addition, we analyzed HPV infection status and compared it to such immunohistochemical parameters. We also analyzed the relationship between these biological products and their clinicopathologic characteristics.Methods. Paraffin-embedded tissue sections were obtained from 84 patients with carcinoma of the uterine cervix. Expression of HSP70, p53, and ER was evaluated by immunohistochemical staining using anti-HSP70 monoclonal antibody (SPA810), anti-p53 (BP53.12), and ER1D5 antibody, respectively. PCR HPV detection was done by dot hybridization method.Results. Positive staining of HSP70 was detected in 73% of the cases. HSP70 positivity was significantly higher in stage I cervical cancer than in stages II–IV (P = 0.02). This was associated with neither tumor size, lymph node status, parametrial involvement status, nor tumor markers (TA-4). Furthermore, there was no significant correlation between HSP70 positivity and the expression of p53 or ER or HPV infection status.Conclusion. These data suggested that HSP70 positivity was frequent in uterine cervical cancer, especially in the early stages. However, this was not significantly correlated with clinicopathologic characteristics nor with the expression of p53 or ER nor with HPV infection in carcinoma of the uterine cervix.
    Gynecologic Oncology 08/1999; 74(1-74):53-60. DOI:10.1006/gyno.1999.5429 · 3.69 Impact Factor
  • Menopause 01/1998; 5(4). DOI:10.1097/00042192-199805040-00129 · 2.81 Impact Factor
  • Menopause 01/1998; 5(4):280. DOI:10.1097/00042192-199805040-00130 · 2.81 Impact Factor
  • Menopause 01/1997; 4(4). DOI:10.1097/00042192-199704040-00049 · 2.81 Impact Factor

Publication Stats

2k Citations
296.86 Total Impact Points

Institutions

  • 2000–2012
    • Sungkyunkwan University
      • • Samsung Medical Center
      • • Molecular Therapy Research Center
      • • School of Medicine
      Seoul, Seoul, South Korea
  • 2009
    • Korea Basic Science Institute KBSI
      Sŏul, Seoul, South Korea
  • 2005
    • Johns Hopkins University
      Baltimore, Maryland, United States
    • Samsung Medical Center
      • Department of Obstetrics and Gynecology
      Sŏul, Seoul, South Korea
    • Gyeongsang National University
      • Division of Applied Life Science
      Shinshū, Gyeongsangnam-do, South Korea