[show abstract][hide abstract] ABSTRACT: Purpose: This study was carried out to investigate the efficacyof electric stimulation before and/or after intracytoplasmicsperm injection (ICSI) on bovine oocyte activation andembryo development.
Methods: The oocytes were treated with electric shock before(B), before and after (B&A), and after (A) sperm injection.In each group, sham ICSI (ICSI-s) was performed to excludethe effect of parthenogenesis (B ICSI-s, B&A ICSI-s, and AICSI-s). An electric pulse was applied with a single directcurrent (DC) pulse (0.8 kV/cm, 70 sec).
Results: One pronucleus (PN) formation in the B&A ICSI-sgroup was slightly higher than that found in B and B&AICSI group; however, the difference was not significant. TwoPN formation in B&A ICSI group was higher than that foundin sham ICSI groups (P < 0.05).="" there="" were="" no="" differencesamong="" treatment="" groups="" in="" the="" cleavage="" rate;="" however,="" morulaeand="" blastocyst="" formation="" in="" the="" b&a="" embryos="" wassignificantly="" higher="" than="" that="" of="" other="" groups="">P < 0.05)and="" got="">
Conclusions: Electric stimulation before and after injectionwas an effective method in inducing bovine oocyte activationand in sustaining embryo development to the morulae andblastocyst stage.
Journal of Assisted Reproduction and Genetics 06/2000; 17(6):310-314. · 1.82 Impact Factor
[show abstract][hide abstract] ABSTRACT: Objectives. It has been shown that heat shock proteins (HSPs) protect cells from death caused by various noxious stimuli. Overexpression of HSP70 seems to be related to hormonal regulation of cell proliferation and/or down-regulation of sex steroid receptors. Wild-type p53 has been reported to repress HSP70 gene expression. It has been shown that mutant p53–HSP70 complex is highly expressed in cancer. However, the relationship between HSPs and steroid receptors or tumor suppressor gene products has not been well understood in uterine cervical carcinoma. This study was undertaken to examine the expression of HSP70, estrogen receptor (ER), and p53 in carcinoma of the uterine cervix. In addition, we analyzed HPV infection status and compared it to such immunohistochemical parameters. We also analyzed the relationship between these biological products and their clinicopathologic characteristics.Methods. Paraffin-embedded tissue sections were obtained from 84 patients with carcinoma of the uterine cervix. Expression of HSP70, p53, and ER was evaluated by immunohistochemical staining using anti-HSP70 monoclonal antibody (SPA810), anti-p53 (BP53.12), and ER1D5 antibody, respectively. PCR HPV detection was done by dot hybridization method.Results. Positive staining of HSP70 was detected in 73% of the cases. HSP70 positivity was significantly higher in stage I cervical cancer than in stages II–IV (P = 0.02). This was associated with neither tumor size, lymph node status, parametrial involvement status, nor tumor markers (TA-4). Furthermore, there was no significant correlation between HSP70 positivity and the expression of p53 or ER or HPV infection status.Conclusion. These data suggested that HSP70 positivity was frequent in uterine cervical cancer, especially in the early stages. However, this was not significantly correlated with clinicopathologic characteristics nor with the expression of p53 or ER nor with HPV infection in carcinoma of the uterine cervix.
[show abstract][hide abstract] ABSTRACT: This study was undertaken to investigate cyclin D2 mRNA and protein expression in human endometrium during the menstrual cycle.
Endometrial samples were obtained from 15 premenopausal nonpregnant women who had hysterectomies for benign gynecologic reasons. They were divided into the following five groups according to histologic dating: early proliferative (n = 3), mid to late proliferative (n = 3), early secretory (n = 3), mid secretory (n = 3), and late secretory (n = 3). Cyclin D2 mRNA and protein expression were analyzed using reverse transcriptase-polymerase chain reaction, Western blotting, and immunohistochemistry.
Cyclin D2 mRNA and protein were expressed in human endometrial tissue throughout the menstrual cycle. Cyclin D2 mRNA and protein expression of proliferative phase endometrium were significantly higher than those of secretory phase endometrium (P <.05). The staining intensity of cyclin D2 in proliferative phase endometrium was higher than that in secretory phase (P <.05). Cyclin D2 mRNA level showed good correlation with cyclin D2 protein level (R = 0.579, P <.03), and cyclin D2 protein also showed good correlation with immunohistochemical staining intensity (R = 0.562, P <.03).
Cyclin D2 was expressed in human endometrium throughout the menstrual cycle. Cyclin D2 mRNA and protein were expressed at high levels in proliferative phase endometrium, especially in the early proliferative phase, and then decreased in the secretory phase.
Journal of the Society for Gynecologic Investigation 9(1):41-6. · 2.26 Impact Factor