Alicia Piera Alberola

Publications (6)32.59 Total impact

• Source

  Available from: Martin Oheim

  Article: Getting across the plasma membrane and beyond: intracellular uses of colloidal semiconductor nanocrystals.

  Camilla Luccardini, Aleksey Yakovlev, Stéphane Gaillard, Marcel van 't Hoff, Alicia Piera Alberola, Jean-Maurice Mallet, Wolfgang J Parak, Anne Feltz, Martin Oheim
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  ABSTRACT: Semiconductor nanocrystals (NCs) are increasingly being used as photoluminescen markers in biological imaging. Their brightness, large Stokes shift, and high photostability compared to organic fluorophores permit the exploration of biological phenomena at the single-molecule scale with superior temporal resolution and spatial precision. NCs have predominantly been used as extracellular markers for tagging and tracking membrane proteins. Successful internalization and intracellular labelling with NCs have been demonstrated for both fixed immunolabelled and live cells. However, the precise localization and subcellular compartment labelled are less clear. Generally, live cell studies are limited by the requirement of fairly invasive protocols for loading NCs and the relatively large size of NCs compared to the cellular machinery, along with the subsequent sequestration of NCs in endosomal/lysosomal compartments. For long-period observation the potential cytotoxicity of cytoplasmically loaded NCs must be evaluated. This review focuses on the challenges of intracellular uses of NCs.
  Journal of Biomedicine and Biotechnology 02/2007; 2007(7):68963. · 2.44 Impact Factor
• Source

  Available from: Martin Oheim

  Article: Getting across the plasma membrane and beyond: intracellular uses of colloidal semiconductor nanocrystals

  Camilla Luccardini, Aleksey Yakovlev, S Ephane Gaillard, Marcel Van 't Hoff, Alicia Piera Alberola, Jean-Maurice Mallet, Wolfgang J Parak, Anne Feltz, Martin Oheim
  [show abstract] [hide abstract]
  ABSTRACT: Semiconductor nanocrystals (NCs) are increasingly being used as photoluminescen markers in biological imaging. Their bright-ness, large Stokes shift, and high photostability compared to organic fluorophores permit the exploration of biological phenomena at the single-molecule scale with superior temporal resolution and spatial precision. NCs have predominantly been used as extra-cellular markers for tagging and tracking membrane proteins. Successful internalization and intracellular labelling with NCs have been demonstrated for both fixed immunolabelled and live cells. However, the precise localization and subcellular compartment labelled are less clear. Generally, live cell studies are limited by the requirement of fairly invasive protocols for loading NCs and the relatively large size of NCs compared to the cellular machinery, along with the subsequent sequestration of NCs in endo-somal/lysosomal compartments. For long-period observation the potential cytotoxicity of cytoplasmically loaded NCs must be evaluated. This review focuses on the challenges of intracellular uses of NCs.
  Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology Article ID. 01/2007; 68963(9).
• Source

  Available from: uba.ar

  Article: Laser-induced release of encapsulated materials inside living cells.

  Andre G Skirtach, Almudena Muñoz Javier, Oliver Kreft, Karen Köhler, Alicia Piera Alberola, Helmuth Möhwald, Wolfgang J Parak, Gleb B Sukhorukov
  Angewandte Chemie International Edition 08/2006; 45(28):4612-7. · 13.45 Impact Factor
• Article: Laser‐Induced Release of Encapsulated Materials inside Living Cells

  Andre G. Skirtach Dr, Almudena Muñoz Javier, Oliver Kreft Dr, Karen Köhler, Alicia Piera Alberola, Helmuth Möhwald Prof. Dr, Wolfgang J. Parak Dr, Gleb B. Sukhorukov Prof. Dr
  Angewandte Chemie 06/2006; 118(28):4728 - 4733.
• Article: Combined atomic force microscopy and optical microscopy measurements as a method to investigate particle uptake by cells.

  Almudena Muñoz Javier, Oliver Kreft, Alicia Piera Alberola, Christian Kirchner, Bernd Zebli, Andrei S Susha, Elias Horn, Susanne Kempter, Andre G Skirtach, Andrey L Rogach, Joachim Rädler, Gleb B Sukhorukov, Martin Benoit, Wolfgang J Parak
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  ABSTRACT: We propose a combination of atomic force microscopy (AFM) and optical microscopy for the investigation of particle uptake by cells. Positively and negatively charged polymer microcapsules were chosen as model particles, because their interaction with cells had already been investigated in detail. AFM measurements allowed the recording of adhesion forces on a single-molecule level. Due to the micrometer size of the capsules, the number of ingested capsules could be counted by optical microscopy. The combination of both methods allowed combined measurement of the adhesion forces and the uptake rate for the same model particle. As a demonstration of this system, the correlation between the adhesion of positively or negatively charged polymer microcapsules onto cell surfaces and the uptake of these microcapsules by cells has been investigated for several cell lines. As is to be expected, we find a correlation between both processes, which is in agreement with adsorption-dependent uptake of the polymer microcapsules by cells.
  Small 04/2006; 2(3):394-400. · 8.35 Impact Factor
• Article: Combined Atomic Force Microscopy and Optical Microscopy Measurements as a Method To Investigate Particle Uptake by Cells

  Almudena Muñoz Javier, Oliver Kreft Dr, Alicia Piera Alberola, Christian Kirchner Dr, Bernd Zebli, Andrei S. Susha Dr, Elias Horn, Susanne Kempter, Andre G. Skirtach Dr, Andrey L. Rogach Dr, Joachim Rädler Prof, Gleb B. Sukhorukov Prof, Martin Benoit Dr, Wolfgang J. Parak Dr
  Small 01/2006; 2(3):394 - 400. · 8.35 Impact Factor