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ABSTRACT: A link between homocysteine (Hcy) and left ventricular ejection fraction (LVEF) emerged from recent studies but was yet not explored specifically in diabetic patients. This study aimed to assess the relationship between LVEF and Hcy in a cohort of adults with diabetes.
LVEF was determined through the measure of left ventricular end-diastolic/end-systolic volumes in 409 consecutive asymptomatic patients with diabetes who underwent stress myocardial perfusion imaging. Clinical and biological parameters which were determinants of LVEF in univariate analyses with p<0.15 were included in a multivariate analysis.
In univariate analyses, factors significantly associated with a LVEF<55% were gender [women vs. men, odds ratio (OR)=0.22 (0.13; 0.38)], peripheral arterial disease [OR=2.49 (1.34; 4.62)], active smoking [OR=1.97 (1.16; 3.33)], silent myocardial ischemia (SMI) [OR=2.19 (1.25; 3.86)], the highest vs. the lowest tertile of creatinine [OR=2.08 (1.17; 3.68)], of albuminuria [OR=2.22 (1.27; 3.90)] and of Hcy [OR=1.83 (1.07; 3.13)]. No relationship was observed between blood pressure and decrease in LVEF. In the multivariate analysis, female gender was confirmed as being protective for having a LVEF<55%. Presence of SMI [OR=2.20 (1.14; 4.23)] and Hcy ≥15 μmol/L [OR=1.81 (1.06; 3.07)] were the two remaining significant factors associated with an increased relative risk of having LVEF<55%. A trend was only observed for the criteria active smokers.
This study highlights an inverse relationship between Hcy and LVEF in patients with diabetes, independent of age, gender, SMI, smoking, blood pressure, renal function, folates, vitamin B12, lipid parameters and hepatic enzymes.
Clinical Chemistry and Laboratory Medicine 01/2012; 50(6):1099-106. · 2.15 Impact Factor
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Absalome A Monde,
Françoise Michel,
Marie-Annette Carbonneau,
Georges Tiahou,
Marie-Hélène Vernet,
Sabrina Eymard-Duvernay, Stephanie Badiou,
Benjamin Adon,
Eugène Konan,
Daniel Sess,
Jean Paul Cristol
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ABSTRACT: BACKGROUND: Fatty acid, tocopherol, tocotrienol and carotene contents were assessed in four oil palm species from the National Centre of Agronomical Research of Côte d'Ivoire, two of which were the basal Lamé (HP1) and Deli (HP2) collections and two of which resulted from crossings between HP1 and HP2 varieties of Eleais guineensis, HP3 and HP4 being identified as the first and second cycle selection, respectively.RESULTS: Palm oil species were characterized by the richness in polyunsaturated fatty acids composition (48–60%) compared to saturated fatty acids (40–52%), especially the first variety, which was from the base collection, and the two hybrids ensuing from crossing. Total carotene content of those varieties was higher and accounted for 832–3575 µg g−1, and the β-carotene level (580–2390 µg g−1) was predominant. Total vitamin E content was 864–1124 µg g−1, with a notable higher content of tocotrienols, especially γ-and α-tocotrienol, ranging from 400 to 515 µg g−1 and from 238 to 350 µg g−1, respectively.CONCLUSION: Crossing seemed to be useful in improving the performance and analytical characteristics of the base collection materials. Copyright © 2009 Society of Chemical Industry
Journal of the Science of Food and Agriculture 10/2009; 89(15):2535 - 2540. · 1.44 Impact Factor
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ABSTRACT: In the present study, homocysteine (Hcy) and vitamin B status were determined in healthy subjects living in two opposite regions of the Ivory Coast. Fifty-six subjects from a coastal region (Bodou) having a fish-based diet and 56 subjects from a mountainous area (Glanle) having a vegetarian diet were tested to sample Hcy, folate, vitamin B12, creatinine, and lipid levels, as well as inflammation and nutritional parameters. An increased prevalence of Hcy > or = 15 micromol/L was observed, reaching 60 % of all subjects. The Bodou group exhibited significantly higher Hcy levels than the Glanle group [20.1 (9.7 - 41.4) vs. 13.6 (5.5 - 48.7) micromol/L, p<0.0001], despite higher vitamin B12 levels [593 (163 - 1860) vs. 234 (83 - 585) pg/mL, p<0.0001]. Although folate levels were lower in subjects from Bodou compared to Glanle [3.2 (2.0 - 7.3) vs. 6.0 (1.9 - 18.2) ng/mL, p<0.0001], there was no significant relationship with Hcy levels in any groups. Interestingly, there were significantly higher creatinine levels in subjects from Bodou compared to Glanle and a significant positive relationship with Hcy levels was evident in each group. In conclusion, Hcy levels in an Ivorian population having a fish-based diet appeared significantly higher compared to subjects having a vegetarian diet. However, folate and vitamin B12 status did not emerge as the major determinants of this difference; a stronger relationship was observed with creatinine levels.
International Journal for Vitamin and Nutrition Research 09/2009; 79(5-6):319-27. · 0.88 Impact Factor
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ABSTRACT: This study was conducted to evaluate the analytical performance of a new colorimetric assay for plasma homocysteine (Hcy) and to determine the influence of the method used on the frequency of hyperhomocysteinemia and on the association between Hcy and biological parameters in diabetic patients. Plasma Hcy was measured in 160 diabetic patients by high performance liquid chromatography (HPLC) in parallel with an automated colorimetric assay (DiaSys Diagnostic Systems GmbH) using the Olympus AU640 and Abbott C8000 analyzers. Plasma glucose, creatinine, folates, vitamin B(12), high-sensitivity C-reactive protein (hs-CRP), and blood hemoglobin A1c (HbA1c) concentrations were measured by routine methods. The Hcy concentrations obtained with the HPLC (x) and colorimetric methods (y) were highly correlated (y = 1.032x - 0.65, r(2) = 0.884 for AU640; y = 1.028x + 0.87, r(2) = 0.934 for C8000). The frequency of Hcy >15 micromol/L was 41.9% by HPLC assay and there was no statistically significant difference using the colorimetric method. Patients with Hcy >15 micromol/L compared to < or =15 micromol/L using HPLC had significantly lower glycemia (p <0.001), lower HbA1c (p <0.001), lower estimated glomerular filtration rate (GFR) (p <0.0001), and lower vitamin B(12) levels (p <0.001). All these relationships were also noted when using the colorimetric assay on the Olympus AU640 or Abbott C8000 analyzers. In summary, this study shows that the DiaSys colorimetric assay is suitable for plasma Hcy determinations in routine clinical biochemistry.
Annals of clinical and laboratory science 02/2009; 39(3):233-40. · 0.96 Impact Factor
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Thomas Bertsch,
Christine Aschenneller,
Verena Herzog,
Anne Marie Dupuy,
Anne Sophie Bargnoux, Stephanie Badiou,
Anne Ebel,
Angela Winter,
Adeltraud Graf,
Stefan Rauch,
Jürgen Durner,
Stefan Holdenrieder,
Peter Fraunberger,
Raymond Gamble,
Brian Sheridan,
Janine Kukula,
Paul Kenny,
Jean Paul Cristol
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ABSTRACT: The AU3000i thyroid assay panel (TSH, fT4, T4, fT3, T3) was evaluated at four sites in a European multicenter study. The study was designed to assess the basic analytical performance characteristics of the Olympus thyroid assays. In addition, a comprehensive assessment of the TSH functional sensitivity was undertaken to challenge the manufacturer's claim of 4th generation assay performance.
Repeatability (within-run precision) of TSH, ff4 and T4 was better than 3% across the measurable range, T3 and fT3 repeatability was better than 6%. Within-laboratory (total) precision was better than 10% for all assays, for fT4, it was better than 3%. Method comparisons were undertaken against the Roche Elecsys 2010, the Siemens Advia Centaur and the Abbott Architect. Overall, good to excellent correlations were seen, however in some cases there were systematic differences which can be attributed to the lack of an appropriate standard or reference method and/or heterogeneity of the analyte. The functional sensitivity of the Olympus TSH assay was confirmed to be 4th generation, giving a mean functional sensitivity (at 20% CV) of 0.0011 mIU/L with no sites exceeding 0.002 mIU/L. Plasma (Li-heparinate) was shown to be an acceptable sample type for use in these assays.
The results generated in this study indicate that the assays of the Olympus AU3000i routine thyroid panel are precise, correlate well with other established assays, and are suitable for use in the routine clinical laboratory.
Clinical laboratory 01/2009; 55(11-12):447-58. · 0.90 Impact Factor
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ABSTRACT: A new immunoanalyzer (AU3000i) has been developed by Olympus (Rungis, France) with the intention of offering a consolidated workcell. The aim of this experiment was to assess, in a multi-centre study (two French sites, one German site), the practicability of the Olympus AU3000i in terms of throughput (test/h), rerun capabilities, emergency sample handling and reflex test capabilities.
The workload study showed that the AU3000i was capable of running both one- and two-step assay protocols with a throughput of 240 tests per hour which corresponds to 209.3 tests per hour including the initialization time. Emergency samples were correctly treated as a priority in less than 30 minutes for ten samples. Furthermore the analyzer could be programmed to generate automatic dilution, rerun and reflex tests, reducing the hands-on labour time for technicians.
Workflow studies showed that Olympus AU3000i and AU Clinical Chemistry can cover the combined workload of various routine analyzers in a variety of laboratory environments.
Clinical laboratory 01/2009; 55(11-12):459-64. · 0.90 Impact Factor
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ABSTRACT: Reactive oxygen species production increases during aging, whereas protective mechanisms such as heat shock proteins (HSPs) or antioxidant capacity are depressed. Physical activity has been hypothesized to provide protection against oxidative damage during aging, but results remain controversial. This study aimed to investigate the effect of different levels of physical activity during aging on Hsp72 expression and systemic oxidative stress at rest and in response to maximal exercise.
Plasma antioxidant capacity (Trolox equivalent antioxidant capacity, TEAC), thiobarbituric acid-reactive species (TBARS), advanced oxidized proteins products (AOPP), and Hsp72 expression in leukocytes were measured before and after maximal exercise testing in 32 elderly persons (aged 73.2 years), who were assigned to two different groups depending on their level of physical activity during the past 12 months (OLow = moderate to low level; OHigh = higher level).
The OHigh group showed higher aerobic fitness and TEAC (both representing 120% of OLow values) as well as lower oxidative damage (50% of OLow values) and Hsp72 expression. Exercise led to a lower increase in oxidative damage in the OHigh group. Aerobic fitness was positively correlated with TEAC and negatively with lipid peroxidation (TBARS). Hsp72 expression was negatively correlated with TEAC but positively correlated with TBARS levels.
The key finding of this study is that, in people aged 60 to 90 years, long-term high level of physical activity preserved antioxidant capacity and limited oxidative damage accumulation. It also downregulated Hsp72 expression, an adaptation potentially resulting from lower levels of oxidative damage.
The Journals of Gerontology Series A Biological Sciences and Medical Sciences 01/2008; 62(12):1413-9. · 4.60 Impact Factor
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ABSTRACT: Low HDL-cholesterol, hypertriglyceridemia (HTG) and occurrence of small dense LDL could be involved in increased cardiovascular risk in HIV-infected patients. This study evaluates the effects of fenofibrate and/or Vitamin E on lipoprotein profile.
Thirty-six HIV-positive adults with fasting triglycerides (TGs) > or =2 mmol/l and stable antiretroviral therapy (ART) were randomly assigned to receive either micronised fenofibrate (200 mg/day) or Vitamin E (500 mg/day) for a first period of 3 months and the association of both for an additional 3-month period.
Total cholesterol, HDL-C, LDL-C, triglycerides, apoA1, apoB, apoCIII, lipoprotein composition, LDL size and LDL resistance to copper-induced oxidation were determined before initiation of fenofibrate or Vitamin E, and 3 and 6 months thereafter. Three months of fenofibrate treatment results in a significant decrease in triglycerides (-40%), apoCIII (-21%), total cholesterol (-14%), apoB (-17%) levels, non-HDL-C (-17%), TG/apoA1 ratio in HDL (-27%) associated with an increase in HDL-C (+15%) and apoA1 (+11%) levels. Moreover, fenofibrate increases LDL size and enhances LDL resistance to oxidation. Three months of Vitamin E supplementation only improves LDL resistance to oxidation and addition to fenofibrate results in a slightly greater effect.
Fenofibrate therapy improves the atherogenic lipid profile in HIV-positive adults with hypertriglyceridemia.
Atherosclerosis 02/2004; 172(2):273-9. · 3.79 Impact Factor
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ABSTRACT: Amino acid analysis in physiological fluids remains expensive, as it usually requires a dedicated analyzer. We modified an RP-HPLC method originally devoted to peptide and protein hydrolysate analysis in order to apply it to plasma amino acid determination. The described method uses a commercial kit based on 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate derivatization followed by reversed-phase high-performance liquid chromatography. The described method is easy to use and allows the determination of twelve amino acids frequently involved in aminoacidopathies, thus enabling the diagnosis and follow-up of affected patients. The results obtained by this method are comparable to those obtained with a classical amino acid analyzer.
Clinical laboratory 02/2004; 50(3-4):153-8. · 0.90 Impact Factor
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ABSTRACT: Oxidative stress and alterations in lipid metabolism observed in hemodialysis patients potentiate the low-density lipoprotein (LDL) oxidability, recognized as a key event during early atherogenesis.
To explore the effects of an oral vitamin E supplementation on oxidative stress markers and LDL oxidability in hemodialysis patients.
Fourteen hemodialysis patients and six healthy volunteers were given oral vitamin E (500 mg/day) for six months. Oxidative stress was assessed using: plasma and lipoprotein vitamin E levels [high-performance liquid chromatography (HPLC) procedure]; thiobarbituric acid reactive substances (TBARS, Yaggi method); and copper-induced LDL oxidation. All parameters were evaluated before initiation of vitamin E supplementation, and at three and six months thereafter.
At baseline, a significantly higher TBARS concentration and a higher LDL oxidability were observed in hemodialysis patients when compared to controls. After six months of vitamin E supplementation, TBARS and LDL oxidability were normalized in hemodialysis patients.
Our data confirm that hemodialysis patients are exposed to oxidative stress and increased susceptibility to ex vivo LDL oxidation. Since oral vitamin E supplementation prevents oxidative stress and significantly increases LDL resistance to ex vivo oxidation, supplementation by natural antioxidants such as vitamin E may be beneficial in hemodialysis patients.
International Journal for Vitamin and Nutrition Research 08/2003; 73(4):290-6. · 0.88 Impact Factor
